Rorug04G0075100

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003224
Physical Location & Seq
Forward (+)
11603966 .. 11604570
605 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0075100.1

Sequence Viewer

Length: 396 bp
ATGCCATCCCTAGTGGAGGCAGCAGTGAGTCCACTTTACACCTGTGTAGAGATTGCCCTTTTACGAGGCAGAGATGCCGCTCGTCTACAAGAGTTTCGAGTTCATAATACCTACTCTGCTCTGGCTAGTGCTAGAAGAAGAAGAGTGCAAACATGGGTGGCTCCATCAGCAGGAGTGTTAAAAATCAATGTTGATGGCTCCTTTCATCATAACACTAGGTCTGGCGGGTTTGGTTTTGTGATTAGAGATTCAAGTGGTGCAATGTTAGCTGGAGGTGTAGGCCCTTTATGTGGACTGATATCATCAGAACATGCCGAAGTTTTGGCGTGTAAGAATGCAATTAATCATGGCTTCATTCCAGCTCTTTTGGAGACTGAGGCACAAGAGGTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

131

Amino Acids

13.98

Weight (kDa)

7.81

Isoelectric Point (pI)

53.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 63 - 116 2.2e-08 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 80
AccI GTMKAC 1 cut(s) 85
AciI CCGC 2 cut(s) 78, 225
AfiI CCNNNNNNNGG 3 cut(s) 16, 170, 290
AgsI TTSAA 1 cut(s) 252
AluBI AGCT 2 cut(s) 269, 362
AluI AGCT 2 cut(s) 269, 362
Alw26I GTCTC 1 cut(s) 365
AoxI GGCC 1 cut(s) 280
ApeKI GCWGC 1 cut(s) 20
AseI ATTAAT 1 cut(s) 342
AspS9I GGNCC 1 cut(s) 281
BbvI GCAGC 1 cut(s) 32
BccI CCATC 3 cut(s) 13, 172, 188
BcoDI GTCTC 1 cut(s) 365
BfaI CTAG 4 cut(s) 11, 126, 132, 216
BisI GCNGC 2 cut(s) 21, 78
BlsI GCNGC 2 cut(s) 22, 79
BmgT120I GGNCC 1 cut(s) 281
BmiI GGNNCC 2 cut(s) 162, 199
BmsI GCATC 1 cut(s) 64
BpmI CTGGAG 1 cut(s) 291
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 170, 290
Bse3DI GCAATG 1 cut(s) 267
BseGI GGATG 1 cut(s) 5
BseLI CCNNNNNNNGG 3 cut(s) 16, 170, 290
BseMI GCAATG 1 cut(s) 267
BseMII CTCAG 1 cut(s) 366
BseXI GCAGC 1 cut(s) 32
BshFI GGCC 1 cut(s) 282
BslI CCNNNNNNNGG 3 cut(s) 16, 170, 290
BsmAI GTCTC 1 cut(s) 365
BsmI GAATGC 1 cut(s) 340
BsnI GGCC 1 cut(s) 282
BspACI CCGC 2 cut(s) 78, 225
BspANI GGCC 1 cut(s) 282
BspCNI CTCAG 1 cut(s) 367
BspLI GGNNCC 2 cut(s) 162, 199
BsrBI CCGCTC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 267
Bst6I CTCTTC 1 cut(s) 136
BstDEI CTNAG 1 cut(s) 375
BstENI CCTNNNNNAGG 1 cut(s) 14
BstF5I GGATG 1 cut(s) 5
BstMAI GTCTC 1 cut(s) 365
BstMWI GCNNNNNNNGC 2 cut(s) 167, 266
BstNSI RCATGY 1 cut(s) 314
BstV1I GCAGC 1 cut(s) 32
BsuRI GGCC 1 cut(s) 282
BtsCI GGATG 1 cut(s) 5
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 1 cut(s) 30
Cfr13I GGNCC 1 cut(s) 281
CviAII CATG 3 cut(s) 153, 311, 347
CviJI RGCY 7 cut(s) 125, 161, 198, 269, 282, 351, 362
CviKI_1 RGCY 7 cut(s) 125, 161, 198, 269, 282, 351, 362
DdeI CTNAG 1 cut(s) 375
Eam1104I CTCTTC 1 cut(s) 136
EarI CTCTTC 1 cut(s) 136
Eco32I GATATC 1 cut(s) 300
EcoNI CCTNNNNNAGG 1 cut(s) 14
EcoO109I RGGNCCY 1 cut(s) 281
EcoRV GATATC 1 cut(s) 300
FaeI CATG 3 cut(s) 156, 314, 350
FaiI YATR 6 cut(s) 105, 154, 210, 289, 312, 348
FatI CATG 3 cut(s) 152, 310, 346
FauI CCCGC 1 cut(s) 218
FblI GTMKAC 1 cut(s) 85
Fnu4HI GCNGC 2 cut(s) 21, 78
Fsp4HI GCNGC 2 cut(s) 21, 78
FspBI CTAG 4 cut(s) 11, 126, 132, 216
GluI GCNGC 2 cut(s) 21, 78
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 1 cut(s) 282
Hin1II CATG 3 cut(s) 156, 314, 350
HinfI GANTC 2 cut(s) 28, 248
Hpy166II GTNNAC 3 cut(s) 32, 86, 293
Hpy188I TCNGA 1 cut(s) 307
Hpy8I GTNNAC 3 cut(s) 32, 86, 293
HpyCH4V TGCA 4 cut(s) 148, 260, 338, 391
HpyF10VI GCNNNNNNNGC 2 cut(s) 167, 266
HpyF3I CTNAG 1 cut(s) 375
Hsp92II CATG 3 cut(s) 156, 314, 350
LmnI GCTCC 2 cut(s) 166, 203
LpnPI CCDG 6 cut(s) 55, 107, 156, 207, 255, 372
Lsp1109I GCAGC 1 cut(s) 32
LweI GCATC 1 cut(s) 64
MaeI CTAG 4 cut(s) 11, 126, 132, 216
MbiI CCGCTC 1 cut(s) 80
MboII GAAGA 3 cut(s) 147, 150, 153
MluCI AATT 1 cut(s) 339
MlyI GAGTC 1 cut(s) 37
MnlI CCTC 5 cut(s) 10, 59, 266, 370, 379
MseI TTAA 2 cut(s) 179, 342
Mva1269I GAATGC 1 cut(s) 340
MwoI GCNNNNNNNGC 2 cut(s) 167, 266
NlaIII CATG 3 cut(s) 156, 314, 350
NlaIV GGNNCC 2 cut(s) 162, 199
NspI RCATGY 1 cut(s) 314
PctI GAATGC 1 cut(s) 340
PfeI GAWTC 1 cut(s) 248
PkrI GCNGC 2 cut(s) 22, 79
PleI GAGTC 1 cut(s) 36
PpsI GAGTC 1 cut(s) 36
PshBI ATTAAT 1 cut(s) 342
PspN4I GGNNCC 2 cut(s) 162, 199
PspPI GGNCC 1 cut(s) 281
SaqAI TTAA 2 cut(s) 179, 342
SatI GCNGC 2 cut(s) 21, 78
Sau96I GGNCC 1 cut(s) 281
SchI GAGTC 1 cut(s) 37
SetI ASST 7 cut(s) 44, 113, 221, 271, 277, 364, 390
SfaNI GCATC 1 cut(s) 64
Sse9I AATT 1 cut(s) 339
SsiI CCGC 2 cut(s) 78, 225
SspMI CTAG 4 cut(s) 11, 126, 132, 216
TaqI TCGA 1 cut(s) 97
TasI AATT 1 cut(s) 339
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 1 cut(s) 248
Tru1I TTAA 2 cut(s) 179, 342
Tru9I TTAA 2 cut(s) 179, 342
TscAI CASTG 1 cut(s) 30
TseI GCWGC 1 cut(s) 20
TspDTI ATGAA 3 cut(s) 92, 194, 343
TspRI CASTG 1 cut(s) 30
VspI ATTAAT 1 cut(s) 342
XagI CCTNNNNNAGG 1 cut(s) 14
XceI RCATGY 1 cut(s) 314
XmiI GTMKAC 1 cut(s) 85
XspI CTAG 4 cut(s) 11, 126, 132, 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.