Rroxscaffold_5G00351630

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003224
Physical Location & Seq
Forward (+)
28078601 .. 28091702
13102 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00351630.1

Sequence Viewer

Length: 2988 bp
ATGGGGGTTGTGTCGAGGCGAGTTGTGCCCGCCTGCGGTAGCCTCTGTTTCTTCTGCCCCTCACTGCGTGCAAGGTCAAGGCAACCAGTCAAACGCTACAAGAAGTTGCTCGCTGAGATCTTCCCCCGTAATCAGGATGCTGAACCCAATGACAGAAAGATTGGGAAACTTTGTGAATATGCTCTGAAGAACCCATTGCGAATTCCCAAGATTACTGAGCATCTAGAGCAAAAATGTTACAAGGATTTGCGAAACGAACAGTTTGGATCTGTTAAAGTCATACTGTGCATTTATAGGAAGTTACTATCTTCCTGTAAAGTGCAGATGCCACTATTTGCAAGTAGTCTACTAGGAATTGTTCGGATTCTCTTGGAACAAACCCGACATGATGAAATGCGGATTCTGGGTTGCAAAACACTTGTCGACTTTATAAATAGCCAGATTGATAGCACACACATGTTCAACCTAGAAGGCCTTATTCCTAAACTCTGTGAATTGGCTCAAGAAATGGGAGATGATGAAAGAGCTCTACGTCTACGTTCAGCTGGATTGCAATCACTGGCTTTTATGGTGTGGTTCATGGGTGAGCACTCTCATATCTCGATGGATTTTGACAATATCATATCAGTGACTTTGGAAAATTACGCGGATATGCATACTAAACCTGGGAGTGTGAAAGAAGACGGACAATTTTCTGAATCTCAGCACCAGTGGGCTCAAGAAGTGCTAAAAGCAGAAGTTCATGATTCATCTTTTCCAGATGTTAGTCAGAAGGTTCCTTCATTACCAAACCTGAAAACCCCTGATTTGGACCCCACAATTGATACAGACAAGAGTCCGTCTTATTGGTCAAAGGTTTGCTTGCGTAATATAGCCAGATTGGCAAAGGAAGCTACAACAGTGCGTCGAGTGCTTGAACCCCTTTTCCAAAATTTTGATGCTGCGAACCATTGGTCCCCAGAAAAACCACTTGCCTATTATGTTTTAATGTATCTGCAGTCACTATTAGAGGAATCAGGAGATAACTCTCATCTGTTATTATCCATATTGGTCAAGCACTTGGATCATAAGAATGTTGTCAAACAGCCTCGCTTGCAAACTGATATTGTAAATGTCACCACACAAATAGCACAAAGTGCAAAGCAGCAGGCATCAGTTGCGATTATTGGTGCAATATCTGACCTGATAAAGAATTTACGAAAATGCCTGCAAAATCAAGCTGAAGGGTCCAACCGTAGTAGTACAGATAAATGGAACCAAGATCTTCAGTCGGCACTAGAGAGATGCATCTCGCAACTGTCAAATAAGGTTGGAGATGTGGGGCCCATACTTGATATGATGGCTGTGGTGCTGGAGAATATCCCATCTAGTACTATTGTAGCTAGGGCGACGGTCTCTGCTGTTTATCTAACTGCAAAGATGGTCTCTTCTGTTCCAAACATATCCTATCACAAAAAGCCATTCCCTGATGCTCTGTTTCACCAGTTACTCCTAGCAATGGCCCACGTAGACCATGAAACACGTGTTGGAGCACACAGTATTTTCTCTATTGTTCTTGTGCCATCTCTACTTGTTCCTTGGCTACAGCAGAAAATGAATTCTGTGCAGACTGTTTCTGGCTTTTCATCTGTCAGTAGATTGGACTTTGGAAAGGATGGGAGTTTCTCCATTAAGGATAAAAGAAAAGACACGGGAGCAACCGCGAATGGAGAGTTGAGGGAAGAGGAAAGTCAAATATCGGATGTTTGTGAAAATCAATCCGGCAGCTTCAAGAGTGCTTTGACGGGTGGAAGAGCAGAACTACCTTGCCTTCGGTTAAGCAGTCATCAAGTGAGTCTATTGCTTTCATTAATCTGGGTTCAGGCAACATCTGCAGAGAATACCCCAGCAAATTTTGAGGCGATGGCTCATTCTTATAATGTTGCTTTACTGTTTACCCGATCAAAGGCTTCAAGTCATATGGCTTTAGTAAGATGCTTCCAGCTGGCATTCTCCATCAGGACTATTTCCTTGGATCGAGATGGGGGTTTGCAAGAATCTCGTAGAAGGTCTCTTTATACGTTGGCATCATACATGCTTATATTTGCAGCCCGGGCGGGGAACTTCCCGGAGCTAATTCCCATTGTTAAAGCATCATTGATGGATCAAATGGTTGATCCTTGTCTTCAGTTGGTTGACGATATCCGACTGCAGGCAGTTTCAATTGACTCTGAGATGGAGAAGCTACCCTATGGATCACAGCCAGACGAAGTTGCTGCATTGAAGTCTCATTCAGCAATTGAACTAGATGACCAATTGTTGAAAGAAACCGTGATATCCCACTTCATGACTAAATTTTCCATATTGTCAGAGGATGAATTGTCTAGCATCAAGAAGCAACTCTTACTGGGTTTTTCACCGGATGATGCATTTCCATTAGGAGCTCCACTATTCATGGAGACACCACGACCATGTTCTCCACTTGCCCATATAGATTTTGCTGATTTTGATGAGGTGATGCCTCCAGGTTCTTTGACAGACGAAGAAGCCTTCCCTGAACCTAGTGGAAGTCAGTCAGACCGCAAGACATCACTTTCCATCAACGCACTTGACATTTTAAATGTTAATCAGCTCTTGGATTCAGTTCTAGAAACAGCCCAACAAGTTGCAAGCTTCCCAGTCTCCACAATGCCTGTACCTTATGACCAAATGAAGAGCCAATGTGAAGCCCTTGTAACGGGCAAACAGCAAAAGATGGCAGTTCTTCATAGTTTCAAGCATCAACTAGAAACCAAGGCGTTAGTGCTCTCCAATGTAAATGAAAACAAATCCCATGCTTCACCACCCATGGGATTGGAATCTTCAGAAGGGTACCCAAAGGTCAAAACCAAGGAGCAAATTCAAGCGAAAAACCAGCTCGTCATATGTTCTCGTGAGTATGGACAGCTTTCTTTTAAATTACCACCTTCAAGTCCTTATGACAAATTCTTGAAAGCTGCTGGATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

995

Amino Acids

110.76

Weight (kDa)

6.46

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFR3_ARM PF21052 65 - 151 6.8e-18 EFR3, armadillo repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 431, 1917
Acc65I GGTACC 1 cut(s) 2850
AccB1I GGYRCC 1 cut(s) 2850
AccI GTMKAC 4 cut(s) 346, 423, 535, 1509
AccII CGCG 2 cut(s) 647, 1703
AciI CCGC 7 cut(s) 30, 36, 397, 647, 1701, 2096, 2560
AclWI GGATC 6 cut(s) 274, 1071, 2022, 2150, 2151, 2242
AcsI RAATTY 8 cut(s) 201, 931, 1192, 1597, 1891, 2333, 2877, 2963
AcuI CTGAAG 5 cut(s) 206, 1242, 1250, 2150, 2826
AcvI CACGTG 1 cut(s) 1523
AdeI CACNNNGTG 2 cut(s) 68, 1136
AfaI GTAC 4 cut(s) 1243, 1372, 2676, 2852
AfiI CCNNNNNNNGG 9 cut(s) 35, 133, 808, 1498, 1945, 2097, 2191, 2716, 2828
AflIII ACRYGT 3 cut(s) 456, 1520, 1522
AjnI CCWGG 2 cut(s) 664, 2503
AjuI GAANNNNNNNTTGG 2 cut(s) 1509, 1541
AloI GAACNNNNNNTCC 4 cut(s) 909, 938, 941, 970
Alw21I GWGCWC 5 cut(s) 529, 591, 1534, 2425, 2787
Alw26I GTCTC 6 cut(s) 1399, 1429, 2055, 2271, 2432, 2665
AlwI GGATC 6 cut(s) 274, 1071, 2022, 2150, 2151, 2242
Ama87I CYCGRG 1 cut(s) 2091
AoxI GGCC 3 cut(s) 472, 1322, 1500
ApaI GGGCCC 1 cut(s) 1326
ApeKI GCWGC 6 cut(s) 941, 1144, 1764, 2087, 2255, 2975
ApoI RAATTY 8 cut(s) 201, 931, 1192, 1597, 1891, 2333, 2877, 2963
ArsI GACNNNNNNTTYG 4 cut(s) 405, 437, 938, 970
AseI ATTAAT 1 cut(s) 1850
Asp718I GGTACC 1 cut(s) 2850
AspS9I GGNCC 6 cut(s) 811, 954, 1227, 1322, 1323, 1501
AsuC2I CCSGG 3 cut(s) 2092, 2093, 2108
AsuHPI GGTGA 6 cut(s) 596, 1108, 1472, 2388, 2506, 2811
AvaI CYCGRG 1 cut(s) 2091
AvaII GGWCC 3 cut(s) 811, 954, 1227
BaeGI GKGCMC 2 cut(s) 30, 1326
BanI GGYRCC 1 cut(s) 2850
BanII GRGCYC 4 cut(s) 529, 718, 1326, 2425
BauI CACGAG 1 cut(s) 2910
BbrPI CACGTG 1 cut(s) 1523
BbsI GAAGAC 2 cut(s) 687, 2156
Bbv12I GWGCWC 5 cut(s) 529, 591, 1534, 2425, 2787
BbvI GCAGC 6 cut(s) 928, 1156, 1776, 2099, 2242, 2962
BcgI CGANNNNNNTGC 4 cut(s) 2021, 2055, 2237, 2271
BciT130I CCWGG 2 cut(s) 666, 2505
BcnI CCSGG 3 cut(s) 2092, 2093, 2108
BcoDI GTCTC 6 cut(s) 1399, 1429, 2055, 2271, 2432, 2665
BfmI CTRYAG 4 cut(s) 995, 1583, 1872, 2189
BglI GCCNNNNNGGC 1 cut(s) 881
BglII AGATCT 2 cut(s) 117, 1261
BisI GCNGC 6 cut(s) 942, 1145, 1765, 2088, 2256, 2976
BlsI GCNGC 6 cut(s) 943, 1146, 1766, 2089, 2257, 2977
BmcAI AGTACT 1 cut(s) 1372
Bme1390I CCNGG 5 cut(s) 666, 2092, 2093, 2108, 2505
Bme18I GGWCC 3 cut(s) 811, 954, 1227
BmeT110I CYCGRG 1 cut(s) 2091
BmgT120I GGNCC 6 cut(s) 811, 954, 1227, 1322, 1323, 1501
BmiI GGNNCC 8 cut(s) 777, 813, 956, 1228, 1256, 1323, 1324, 2852
BmrFI CCNGG 5 cut(s) 666, 2092, 2093, 2108, 2505
BmrI ACTGGG 2 cut(s) 2396, 2651
BmuI ACTGGG 2 cut(s) 2396, 2651
BoxI GACNNNNGTC 1 cut(s) 834
BpiI GAAGAC 2 cut(s) 687, 2156
BpmI CTGGAG 2 cut(s) 1373, 2487
BpuEI CTTGAG 2 cut(s) 486, 702
BpuMI CCSGG 3 cut(s) 2092, 2093, 2108
BsaAI YACGTR 2 cut(s) 1507, 1523
BsaBI GATNNNNATC 2 cut(s) 553, 2836
BsaI GGTCTC 3 cut(s) 1399, 1429, 2055
BsaJI CCNNGG 7 cut(s) 665, 1577, 2010, 2091, 2772, 2826, 2868
BsaWI WCCGGW 1 cut(s) 2398
Bsc4I CCNNNNNNNGG 9 cut(s) 35, 133, 808, 1498, 1945, 2097, 2191, 2716, 2828
Bse1I ACTGG 6 cut(s) 86, 564, 709, 1483, 2391, 2657
Bse3DI GCAATG 2 cut(s) 194, 1503
Bse8I GATNNNNATC 2 cut(s) 553, 2836
BseBI CCWGG 2 cut(s) 666, 2505
BseDI CCNNGG 7 cut(s) 665, 1577, 2010, 2091, 2772, 2826, 2868
BseGI GGATG 6 cut(s) 142, 1660, 1747, 2359, 2407, 2987
BseJI GATNNNNATC 2 cut(s) 553, 2836
BseLI CCNNNNNNNGG 9 cut(s) 35, 133, 808, 1498, 1945, 2097, 2191, 2716, 2828
BseMI GCAATG 2 cut(s) 194, 1503
BseMII CTCAG 4 cut(s) 105, 207, 716, 2202
BseNI ACTGG 6 cut(s) 86, 564, 709, 1483, 2391, 2657
BseSI GKGCMC 2 cut(s) 30, 1326
BseXI GCAGC 6 cut(s) 928, 1156, 1776, 2099, 2242, 2962
BseYI CCCAGC 1 cut(s) 1885
BsgI GTGCAG 2 cut(s) 341, 1625
Bsh1236I CGCG 2 cut(s) 647, 1703
BshFI GGCC 3 cut(s) 474, 1324, 1502
BshNI GGYRCC 1 cut(s) 2850
BsiHKAI GWGCWC 5 cut(s) 529, 591, 1534, 2425, 2787
BsiHKCI CYCGRG 1 cut(s) 2091
BsiSI CCGG 4 cut(s) 1761, 2092, 2108, 2399
BslFI GGGAC 1 cut(s) 940
BslI CCNNNNNNNGG 9 cut(s) 35, 133, 808, 1498, 1945, 2097, 2191, 2716, 2828
BsmAI GTCTC 6 cut(s) 1399, 1429, 2055, 2271, 2432, 2665
BsmFI GGGAC 1 cut(s) 940
BsmI GAATGC 1 cut(s) 1988
BsnI GGCC 3 cut(s) 474, 1324, 1502
Bso31I GGTCTC 3 cut(s) 1399, 1429, 2055
BsoBI CYCGRG 1 cut(s) 2091
Bsp120I GGGCCC 1 cut(s) 1322
Bsp1286I GDGCHC 8 cut(s) 30, 529, 591, 718, 1326, 1534, 2425, 2787
Bsp143I GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
Bsp19I CCATGG 1 cut(s) 2826
BspACI CCGC 7 cut(s) 30, 36, 397, 647, 1701, 2096, 2560
BspANI GGCC 3 cut(s) 474, 1324, 1502
BspCNI CTCAG 4 cut(s) 106, 208, 715, 2203
BspFNI CGCG 2 cut(s) 647, 1703
BspHI TCATGA 2 cut(s) 742, 2325
BspLI GGNNCC 8 cut(s) 777, 813, 956, 1228, 1256, 1323, 1324, 2852
BspMAI CTGCAG 3 cut(s) 999, 1876, 2193
BspPI GGATC 6 cut(s) 274, 1071, 2022, 2150, 2151, 2242
BspQI GCTCTTC 2 cut(s) 1786, 2687
BspT107I GGYRCC 1 cut(s) 2850
BspTNI GGTCTC 3 cut(s) 1399, 1429, 2055
BsrDI GCAATG 2 cut(s) 194, 1503
BsrI ACTGG 6 cut(s) 86, 564, 709, 1483, 2391, 2657
BssECI CCNNGG 7 cut(s) 665, 1577, 2010, 2091, 2772, 2826, 2868
BssMI GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
BssSI CACGAG 1 cut(s) 2910
BssT1I CCWWGG 5 cut(s) 1577, 2010, 2772, 2826, 2868
Bst2BI CACGAG 1 cut(s) 2910
Bst2UI CCWGG 2 cut(s) 666, 2505
Bst6I CTCTTC 4 cut(s) 1432, 1716, 1786, 2687
BstAPI GCANNNNNTGC 3 cut(s) 1136, 1157, 1871
BstBAI YACGTR 2 cut(s) 1507, 1523
BstDEI CTNAG 4 cut(s) 114, 216, 702, 2211
BstDSI CCRYGG 1 cut(s) 2826
BstF5I GGATG 6 cut(s) 142, 1660, 1747, 2359, 2407, 2987
BstFNI CGCG 2 cut(s) 647, 1703
BstKTI GATC 9 cut(s) 120, 269, 1066, 1264, 1943, 2017, 2146, 2158, 2237
BstMAI GTCTC 6 cut(s) 1399, 1429, 2055, 2271, 2432, 2665
BstMBI GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
BstNI CCWGG 2 cut(s) 666, 2505
BstNSI RCATGY 2 cut(s) 460, 2077
BstPAI GACNNNNGTC 1 cut(s) 834
BstSCI CCNGG 5 cut(s) 664, 2090, 2091, 2106, 2503
BstSFI CTRYAG 4 cut(s) 995, 1583, 1872, 2189
BstSLI GKGCMC 2 cut(s) 30, 1326
BstUI CGCG 2 cut(s) 647, 1703
BstV1I GCAGC 6 cut(s) 928, 1156, 1776, 2099, 2242, 2962
BstV2I GAAGAC 2 cut(s) 687, 2156
BstX2I RGATCY 3 cut(s) 117, 266, 1261
BstXI CCANNNNNNTGG 1 cut(s) 2833
BstYI RGATCY 3 cut(s) 117, 266, 1261
BsuRI GGCC 3 cut(s) 474, 1324, 1502
BtgI CCRYGG 1 cut(s) 2826
BtgZI GCGATG 1 cut(s) 1916
BtsCI GGATG 6 cut(s) 142, 1660, 1747, 2359, 2407, 2987
BtsI GCAGTG 1 cut(s) 62
BtsIMutI CAGTG 5 cut(s) 62, 557, 633, 716, 906
CciI TCATGA 2 cut(s) 742, 2325
Cfr13I GGNCC 6 cut(s) 811, 954, 1227, 1322, 1323, 1501
Cfr9I CCCGGG 1 cut(s) 2091
CseI GACGC 1 cut(s) 893
Csp6I GTAC 4 cut(s) 1242, 1371, 2675, 2851
CviQI GTAC 4 cut(s) 1242, 1371, 2675, 2851
DdeI CTNAG 4 cut(s) 114, 216, 702, 2211
DpnI GATC 9 cut(s) 119, 268, 1065, 1263, 1942, 2016, 2145, 2157, 2236
DpnII GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
DraI TTTAAA 2 cut(s) 2598, 2935
DraIII CACNNNGTG 2 cut(s) 68, 1136
Eam1104I CTCTTC 4 cut(s) 1432, 1716, 1786, 2687
EarI CTCTTC 4 cut(s) 1432, 1716, 1786, 2687
Ecl136II GAGCTC 2 cut(s) 527, 2423
Eco130I CCWWGG 5 cut(s) 1577, 2010, 2772, 2826, 2868
Eco147I AGGCCT 1 cut(s) 474
Eco24I GRGCYC 4 cut(s) 529, 718, 1326, 2425
Eco31I GGTCTC 3 cut(s) 1399, 1429, 2055
Eco32I GATATC 2 cut(s) 2182, 2316
Eco47I GGWCC 3 cut(s) 811, 954, 1227
Eco53kI GAGCTC 2 cut(s) 527, 2423
Eco57I CTGAAG 5 cut(s) 206, 1242, 1250, 2150, 2826
Eco72I CACGTG 1 cut(s) 1523
Eco88I CYCGRG 1 cut(s) 2091
EcoICRI GAGCTC 2 cut(s) 527, 2423
EcoO109I RGGNCCY 1 cut(s) 1322
EcoRI GAATTC 2 cut(s) 201, 1597
EcoRII CCWGG 2 cut(s) 664, 2503
EcoRV GATATC 2 cut(s) 2182, 2316
EcoT14I CCWWGG 5 cut(s) 1577, 2010, 2772, 2826, 2868
EcoT22I ATGCAT 3 cut(s) 657, 1289, 2410
EcoT38I GRGCYC 4 cut(s) 529, 718, 1326, 2425
ErhI CCWWGG 5 cut(s) 1577, 2010, 2772, 2826, 2868
FalI AAGNNNNNCTT 4 cut(s) 845, 877, 2366, 2398
FaqI GGGAC 1 cut(s) 940
FauI CCCGC 2 cut(s) 37, 2089
FauNDI CATATG 2 cut(s) 1959, 2903
FblI GTMKAC 4 cut(s) 346, 423, 535, 1509
Fnu4HI GCNGC 6 cut(s) 942, 1145, 1765, 2088, 2256, 2976
FokI GGATG 5 cut(s) 149, 1667, 1754, 2366, 2414
FriOI GRGCYC 4 cut(s) 529, 718, 1326, 2425
Fsp4HI GCNGC 6 cut(s) 942, 1145, 1765, 2088, 2256, 2976
GluI GCNGC 6 cut(s) 942, 1145, 1765, 2088, 2256, 2976
GsaI CCCAGC 1 cut(s) 1889
GsuI CTGGAG 2 cut(s) 1373, 2487
HaeIII GGCC 3 cut(s) 474, 1324, 1502
HapII CCGG 4 cut(s) 1761, 2092, 2108, 2399
HgaI GACGC 1 cut(s) 893
HincII GTYRAC 2 cut(s) 424, 2176
HindII GTYRAC 2 cut(s) 424, 2176
HindIII AAGCTT 1 cut(s) 2650
HpaII CCGG 4 cut(s) 1761, 2092, 2108, 2399
HphI GGTGA 6 cut(s) 596, 1108, 1472, 2388, 2506, 2811
Hpy166II GTNNAC 6 cut(s) 347, 424, 536, 1510, 1935, 2176
Hpy8I GTNNAC 6 cut(s) 347, 424, 536, 1510, 1935, 2176
Hpy99I CGWCG 2 cut(s) 909, 1393
HpyAV CCTTC 9 cut(s) 464, 766, 789, 1217, 1820, 2040, 2539, 2840, 2955
HpyCH4IV ACGT 5 cut(s) 532, 538, 1506, 1522, 2060
HpyF3I CTNAG 4 cut(s) 114, 216, 702, 2211
HpySE526I ACGT 5 cut(s) 532, 538, 1506, 1522, 2060
KpnI GGTACC 1 cut(s) 2854
Kzo9I GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
LguI GCTCTTC 2 cut(s) 1786, 2687
LmnI GCTCC 6 cut(s) 1529, 1694, 2110, 2420, 2428, 2872
Lsp1109I GCAGC 6 cut(s) 928, 1156, 1776, 2099, 2242, 2962
MaeII ACGT 5 cut(s) 532, 538, 1506, 1522, 2060
MaeIII GTNAC 7 cut(s) 236, 300, 628, 999, 1114, 1485, 2713
MalI GATC 9 cut(s) 119, 268, 1065, 1263, 1942, 2016, 2145, 2157, 2236
MboI GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
MfeI CAATTG 4 cut(s) 819, 2202, 2277, 2294
MflI RGATCY 3 cut(s) 117, 266, 1261
MhlI GDGCHC 8 cut(s) 30, 529, 591, 718, 1326, 1534, 2425, 2787
MlyI GAGTC 3 cut(s) 844, 1843, 2201
MmeI TCCRAC 4 cut(s) 1254, 1291, 1507, 2209
Mph1103I ATGCAT 3 cut(s) 657, 1289, 2410
MslI CAYNNNNRTG 4 cut(s) 455, 626, 1071, 2449
MspA1I CMGCKG 2 cut(s) 545, 1984
MspI CCGG 4 cut(s) 1761, 2092, 2108, 2399
MspR9I CCNGG 5 cut(s) 666, 2092, 2093, 2108, 2505
MunI CAATTG 4 cut(s) 819, 2202, 2277, 2294
Mva1269I GAATGC 1 cut(s) 1988
MvaI CCWGG 2 cut(s) 666, 2505
MvnI CGCG 2 cut(s) 647, 1703
NciI CCSGG 3 cut(s) 2092, 2093, 2108
NcoI CCATGG 1 cut(s) 2826
NdeI CATATG 2 cut(s) 1959, 2903
NdeII GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
NlaIV GGNNCC 8 cut(s) 777, 813, 956, 1228, 1256, 1323, 1324, 2852
NmuCI GTSAC 3 cut(s) 628, 999, 1114
NsiI ATGCAT 3 cut(s) 657, 1289, 2410
NspI RCATGY 2 cut(s) 460, 2077
PagI TCATGA 2 cut(s) 742, 2325
PceI AGGCCT 1 cut(s) 474
PciI ACATGT 1 cut(s) 456
PciSI GCTCTTC 2 cut(s) 1786, 2687
PctI GAATGC 1 cut(s) 1988
PfeI GAWTC 8 cut(s) 364, 400, 698, 746, 1013, 2036, 2618, 2837
PfoI TCCNGGA 1 cut(s) 2106
PkrI GCNGC 6 cut(s) 943, 1146, 1766, 2089, 2257, 2977
PleI GAGTC 3 cut(s) 843, 1842, 2201
PmaCI CACGTG 1 cut(s) 1523
PmlI CACGTG 1 cut(s) 1523
PpsI GAGTC 3 cut(s) 843, 1842, 2201
Ppu21I YACGTR 2 cut(s) 1507, 1523
PscI ACATGT 1 cut(s) 456
PshAI GACNNNNGTC 1 cut(s) 834
PshBI ATTAAT 1 cut(s) 1850
PsiI TTATAA 2 cut(s) 431, 1917
Psp124BI GAGCTC 2 cut(s) 529, 2425
Psp6I CCWGG 2 cut(s) 664, 2503
PspCI CACGTG 1 cut(s) 1523
PspFI CCCAGC 1 cut(s) 1885
PspGI CCWGG 2 cut(s) 664, 2503
PspN4I GGNNCC 8 cut(s) 777, 813, 956, 1228, 1256, 1323, 1324, 2852
PspOMI GGGCCC 1 cut(s) 1322
PspPI GGNCC 6 cut(s) 811, 954, 1227, 1322, 1323, 1501
PstI CTGCAG 3 cut(s) 999, 1876, 2193
PsuI RGATCY 3 cut(s) 117, 266, 1261
PvuII CAGCTG 2 cut(s) 545, 1984
RsaI GTAC 4 cut(s) 1243, 1372, 2676, 2852
RsaNI GTAC 4 cut(s) 1242, 1371, 2675, 2851
RseI CAYNNNNRTG 4 cut(s) 455, 626, 1071, 2449
SacI GAGCTC 2 cut(s) 529, 2425
SalI GTCGAC 1 cut(s) 422
SapI GCTCTTC 2 cut(s) 1786, 2687
SatI GCNGC 6 cut(s) 942, 1145, 1765, 2088, 2256, 2976
Sau3AI GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
Sau96I GGNCC 6 cut(s) 811, 954, 1227, 1322, 1323, 1501
ScaI AGTACT 1 cut(s) 1372
SchI GAGTC 3 cut(s) 844, 1843, 2201
ScrFI CCNGG 5 cut(s) 666, 2092, 2093, 2108, 2505
SduI GDGCHC 8 cut(s) 30, 529, 591, 718, 1326, 1534, 2425, 2787
SfcI CTRYAG 4 cut(s) 995, 1583, 1872, 2189
SinI GGWCC 3 cut(s) 811, 954, 1227
SmaI CCCGGG 1 cut(s) 2093
SmiMI CAYNNNNRTG 4 cut(s) 455, 626, 1071, 2449
SmlI CTYRAG 2 cut(s) 501, 717
SmoI CTYRAG 2 cut(s) 501, 717
SrfI GCCCGGGC 1 cut(s) 2093
SseBI AGGCCT 1 cut(s) 474
SsiI CCGC 7 cut(s) 30, 36, 397, 647, 1701, 2096, 2560
SstI GAGCTC 2 cut(s) 529, 2425
StuI AGGCCT 1 cut(s) 474
StyD4I CCNGG 5 cut(s) 664, 2090, 2091, 2106, 2503
StyI CCWWGG 5 cut(s) 1577, 2010, 2772, 2826, 2868
TaiI ACGT 5 cut(s) 535, 541, 1509, 1525, 2063
TaqI TCGA 5 cut(s) 14, 423, 602, 907, 2017
TatI WGTACW 2 cut(s) 1241, 1370
TfiI GAWTC 8 cut(s) 364, 400, 698, 746, 1013, 2036, 2618, 2837
TscAI CASTG 5 cut(s) 69, 564, 633, 716, 906
TseFI GTSAC 3 cut(s) 628, 999, 1114
TseI GCWGC 6 cut(s) 941, 1144, 1764, 2087, 2255, 2975
Tsp45I GTSAC 3 cut(s) 628, 999, 1114
TspGWI ACGGA 2 cut(s) 699, 828
TspMI CCCGGG 1 cut(s) 2091
TspRI CASTG 5 cut(s) 69, 564, 633, 716, 906
VpaK11BI GGWCC 3 cut(s) 811, 954, 1227
VspI ATTAAT 1 cut(s) 1850
XapI RAATTY 8 cut(s) 201, 931, 1192, 1597, 1891, 2333, 2877, 2963
XbaI TCTAGA 2 cut(s) 223, 2626
XceI RCATGY 2 cut(s) 460, 2077
XmaI CCCGGG 1 cut(s) 2091
XmiI GTMKAC 4 cut(s) 346, 423, 535, 1509
ZrmI AGTACT 1 cut(s) 1372
Zsp2I ATGCAT 3 cut(s) 657, 1289, 2410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.