Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0011630

Physical Location & Seq

Genomic Coordinates Reverse (-)

33437585 .. 33440360

Gene Structure 2776 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1812 bp

ATGCCTGTCTCTCTCTCTGCCATAAGCATGTTCAAAATTATGTTCTTGGCGGTGGAAATTTTATTCTTCTCAAGCATATTATTAGCAAAAGCTTCTCCTTCACAATCCCATTTCTCCAAATTTTCTAGATCATCCGGAGGACTTTCCCATAAAGAAGCCATTTTATCTCTCTGCGCACATACCCCTCACCGAGCAACTTGTGAATCCACTCTCCTCTCCTGCTCCTCCTCATCATCATCTACAAGAGGCTCTGATCATCTCCCTCATACTCTAGAAGGTGCCTTTGTCCAGTCGGTCCATTCCACCGTTGCGAAAGCTCAGCACGCTCGAGCTCTCGCCTATAATCTCACTATTTCCCACCGCCAAAATGCCGATCATGATGTTCACTTCCCTGGTCGCGGTGTGAATGACTGCCTTGAGCTTCTCGATGATGCTATAGATATGCTCGACAATGTCATGATGACATCGTTGTCAAAACCTAACGATGACGACGTTCACACCTGGCTTAGTGCAGCTCTTACTAACCAAGAAACTTGTCTAGAGAGCCTCGAGAAAGACAAGTTCCAGACAGAGAAAAACATTATGGATGTCACGGCTCAAAATCTGAGCCAATACATAAGCAACTCCCTAGCACTTTTTGTGCTTACCATGGGTAGTAGCAGTGTCTCCTTCGCTAGTAGTTCGGAGCATTTACCTCGAGACACTGAGTCAAAGTCAAAGGAAGCTAAAAATGCAAACCCTAATAGAAGACTATTGGCATCGGACAGCAGTTTTCCGACGTGGGTCCCGGCCTCAGAGAGGAAGCTTCTAGAAGCTTCTGTTGGGGAGATAGAAGCAGATGCTGTGGTGGCTCAGGATGGGAGTGGGACGCACAAGAGCATTGGTGAGGCAGTGAAGGGGTTGCTGCTAATTGGATCCTTGGAGAGTAGTGGTACTAGTACTGGGGGGAGATCTGTGATTTACGTAAAAGCTGGGACATACCACGAGTACATAAACATACCCACGAAGCAAAAGAACGTGATGTTGATTGGTGATGGAGTGGGGAAAACGATCATTGTGGGCAACAGGAACTCAGACGATGGCTGGACAACTTATAAGTCTGCTACTGTGGCTGCTATGGGTGATGGGTTCATCGCTCGTGATATAACATTTGTGAACGATGCCGGCCCTTCCAAGCACCAAGCAGTCGCACTTCGAGTCGGTGCCGACAAATCAGTCGTCCACCGATGTTCCATCATAGGCTACCAGGACACCCTCTACACTCACTCCAAGCGACAGTTCTACCGAGAAACCGACATCTATGGAACCGTCGACTTCATCTTCGGCAACTCAGCCGTGGTTTTCCAAACTTGCAACATCCACCCCCGAAAACCGCCCTCCAGCGGGCTCAAGAACTTTGTCACAGCTCAAGGCCGAAGCAGCCCTAATCAGAACACAGGTATTTCAATCCACAACTGTAAGATAGCGGCGGCATCAGACCTCTTCCCGGTGAGATCCAGCTACGAGACGTATCTTGGGAGACCGTGGCAGCAGTATTCGAGAACGATCATCATGCAGTCTTTCATGGATGACTCGATAAATCGAGCAGGGTGGTCTCCTTGGTCTGGTGGGTCTGCACTTAACACATTGTACTATGGAGAGTACATGAATTCTGGGTCTGGGGCATCAACCTCAGGAAGGGTTAAATGGGGTGGATATCATGCATCACTTACGTCCACTCAAGCAGAGGCATTCACAGTTGGGGGTTTCATATCTGGGAATCTTTGGCTGCCTTCCACTGGGGTGTCTTTTAGTTCTGGACTAATTGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

603

Amino Acids

65.03

Weight (kDa)

6.87

Isoelectric Point (pI)

36.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	53 - 212	2.6e-25	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	280 - 587	5.5e-135	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011630)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43050 AT3G59010
fragaria_vesca	FvH4_4g12140
malus_domestica	MD04G1028900.v1.1 MD04G1029600.v1.1 MD04G1030100.v1.1
prunus_persica	Prupe.1G178400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0409791
rosa_laevigata	RLG00000008522
rosa_multiflora	Rmu_sc0005198.1_g000009 Rmu_sc0040314.1_g000001
rosa_roxburghii	Rroxscaffold_5G00354340
rosa_rugosa	Rorug04G0096600
rosa_samantha	Rh4AG158100 Rh4BG157800 Rh4CG168600 Rh4DG152700
rosa_wichuraiana	Rw0G017480 Rw4G013060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1095
AasI	GACNNNNNNGTC	2 cut(s)	469, 1214
Acc16I	TGCGCA	1 cut(s)	175
AccB1I	GGYRCC	2 cut(s)	278, 1202
AccI	GTMKAC	1 cut(s)	1311
AccII	CGCG	1 cut(s)	399
AccIII	TCCGGA	1 cut(s)	134
AciI	CCGC	7 cut(s)	50, 361, 399, 1373, 1383, 1466, 1469
AclWI	GGATC	3 cut(s)	909, 922, 1488
AcsI	RAATTY	3 cut(s)	57, 119, 1648
AfaI	GTAC	5 cut(s)	934, 940, 989, 1631, 1643
AfiI	CCNNNNNNNGG	8 cut(s)	398, 798, 1382, 1383, 1486, 1604, 1677, 1778
AgsI	TTSAA	2 cut(s)	34, 1446
AhdI	GACNNNNNGTC	1 cut(s)	706
AhlI	ACTAGT	1 cut(s)	935
AjiI	CACGTC	1 cut(s)	780
AjnI	CCWGG	3 cut(s)	391, 500, 1245
AjuI	GAANNNNNNNTTGG	4 cut(s)	804, 836, 1262, 1294
AleI	CACNNNNGTG	1 cut(s)	1781
Alw21I	GWGCWC	1 cut(s)	334
Alw26I	GTCTC	6 cut(s)	13, 670, 693, 1499, 1513, 1599
AlwI	GGATC	3 cut(s)	909, 922, 1488
AlwNI	CAGNNNCTG	1 cut(s)	842
Ama87I	CYCGRG	3 cut(s)	327, 548, 696
Aor13HI	TCCGGA	1 cut(s)	134
AoxI	GGCC	3 cut(s)	789, 1165, 1411
ApeKI	GCWGC	6 cut(s)	512, 904, 1112, 1419, 1528, 1768
ApoI	RAATTY	3 cut(s)	57, 119, 1648
ArsI	GACNNNNNNTTYG	4 cut(s)	1203, 1235, 1304, 1336
AspLEI	GCGC	1 cut(s)	176
AspS9I	GGNCC	3 cut(s)	295, 784, 1166
AsuC2I	CCSGG	2 cut(s)	788, 1487
AsuHPI	GGTGA	5 cut(s)	179, 896, 1043, 1133, 1501
AvaI	CYCGRG	3 cut(s)	327, 548, 696
AvaII	GGWCC	2 cut(s)	295, 784
AxyI	CCTNAGG	1 cut(s)	1672
BamHI	GGATCC	1 cut(s)	914
BanI	GGYRCC	2 cut(s)	278, 1202
BanII	GRGCYC	2 cut(s)	334, 1389
BauI	CACGAG	2 cut(s)	983, 1137
BbsI	GAAGAC	1 cut(s)	754
Bbv12I	GWGCWC	1 cut(s)	334
BbvI	GCAGC	6 cut(s)	524, 891, 1099, 1431, 1540, 1755
BccI	CCATC	5 cut(s)	851, 1028, 1073, 1118, 1241
BceAI	ACGGC	2 cut(s)	609, 1319
BcgI	CGANNNNNNTGC	4 cut(s)	677, 711, 1534, 1568
BciT130I	CCWGG	3 cut(s)	393, 502, 1247
BclI	TGATCA	1 cut(s)	253
BcnI	CCSGG	2 cut(s)	788, 1487
BcoDI	GTCTC	6 cut(s)	13, 670, 693, 1499, 1513, 1599
BcuI	ACTAGT	1 cut(s)	935
BfaI	CTAG	7 cut(s)	126, 272, 539, 629, 675, 809, 936
BfmI	CTRYAG	1 cut(s)	435
BglII	AGATCT	1 cut(s)	950
BisI	GCNGC	8 cut(s)	513, 905, 1113, 1420, 1467, 1470, 1529, 1769
BlpI	GCTNAGC	1 cut(s)	318
BlsI	GCNGC	8 cut(s)	514, 906, 1114, 1421, 1468, 1471, 1530, 1770
BmcAI	AGTACT	1 cut(s)	940
Bme1390I	CCNGG	5 cut(s)	393, 502, 788, 1247, 1487
Bme18I	GGWCC	2 cut(s)	295, 784
BmeRI	GACNNNNNGTC	1 cut(s)	706
BmeT110I	CYCGRG	3 cut(s)	327, 548, 696
BmgBI	CACGTC	1 cut(s)	780
BmgT120I	GGNCC	3 cut(s)	295, 784, 1166
BmiI	GGNNCC	6 cut(s)	280, 785, 786, 916, 1204, 1306
BmrFI	CCNGG	5 cut(s)	393, 502, 788, 1247, 1487
BmrI	ACTGGG	2 cut(s)	951, 1788
BmsI	GCATC	7 cut(s)	421, 767, 829, 1150, 1481, 1673, 1712
BmuI	ACTGGG	2 cut(s)	951, 1788
BoxI	GACNNNNGTC	1 cut(s)	782
BpiI	GAAGAC	1 cut(s)	754
BpmI	CTGGAG	1 cut(s)	1363
Bpu10I	CCTNAGC	1 cut(s)	852
Bpu1102I	GCTNAGC	1 cut(s)	318
BpuEI	CTTGAG	5 cut(s)	55, 437, 1373, 1392, 1704
BpuMI	CCSGG	2 cut(s)	788, 1487
BsaAI	YACGTR	1 cut(s)	964
BsaI	GGTCTC	2 cut(s)	1513, 1599
BsaJI	CCNNGG	6 cut(s)	391, 648, 918, 1335, 1523, 1598
BsaWI	WCCGGW	1 cut(s)	134
BsaXI	ACNNNNNCTCC	2 cut(s)	1250, 1280
Bsc4I	CCNNNNNNNGG	8 cut(s)	398, 798, 1382, 1383, 1486, 1604, 1677, 1778
Bse118I	RCCGGY	1 cut(s)	1163
Bse1I	ACTGG	3 cut(s)	289, 946, 1783
Bse21I	CCTNAGG	1 cut(s)	1672
BseAI	TCCGGA	1 cut(s)	134
BseBI	CCWGG	3 cut(s)	393, 502, 1247
BseDI	CCNNGG	6 cut(s)	391, 648, 918, 1335, 1523, 1598
BseGI	GGATG	5 cut(s)	131, 592, 862, 1356, 1573
BseLI	CCNNNNNNNGG	8 cut(s)	398, 798, 1382, 1383, 1486, 1604, 1677, 1778
BseMII	CTCAG	8 cut(s)	332, 596, 696, 807, 866, 1086, 1344, 1686
BseNI	ACTGG	3 cut(s)	289, 946, 1783
BseRI	GAGGAG	3 cut(s)	203, 214, 217
BseXI	GCAGC	6 cut(s)	524, 891, 1099, 1431, 1540, 1755
BseYI	CCCAGC	1 cut(s)	971
BsgI	GTGCAG	2 cut(s)	531, 1599
Bsh1236I	CGCG	1 cut(s)	399
BshFI	GGCC	3 cut(s)	791, 1167, 1413
BshNI	GGYRCC	2 cut(s)	278, 1202
BsiHKAI	GWGCWC	1 cut(s)	334
BsiHKCI	CYCGRG	3 cut(s)	327, 548, 696
BsiSI	CCGG	4 cut(s)	135, 788, 1164, 1487
BslFI	GGGAC	3 cut(s)	770, 880, 988
BslI	CCNNNNNNNGG	8 cut(s)	398, 798, 1382, 1383, 1486, 1604, 1677, 1778
BsmAI	GTCTC	6 cut(s)	13, 670, 693, 1499, 1513, 1599
BsmBI	CGTCTC	1 cut(s)	1499
BsmFI	GGGAC	3 cut(s)	770, 880, 988
BsmI	GAATGC	1 cut(s)	1730
BsnI	GGCC	3 cut(s)	791, 1167, 1413
Bso31I	GGTCTC	2 cut(s)	1513, 1599
BsoBI	CYCGRG	3 cut(s)	327, 548, 696
Bsp1286I	GDGCHC	2 cut(s)	334, 1389
Bsp13I	TCCGGA	1 cut(s)	134
Bsp143I	GATC	8 cut(s)	128, 253, 373, 914, 950, 1050, 1493, 1545
Bsp1720I	GCTNAGC	1 cut(s)	318
Bsp19I	CCATGG	1 cut(s)	648
BspACI	CCGC	7 cut(s)	50, 361, 399, 1373, 1383, 1466, 1469
BspANI	GGCC	3 cut(s)	791, 1167, 1413
BspCNI	CTCAG	8 cut(s)	331, 597, 697, 806, 865, 1085, 1343, 1685
BspEI	TCCGGA	1 cut(s)	134
BspFNI	CGCG	1 cut(s)	399
BspHI	TCATGA	2 cut(s)	376, 456
BspLI	GGNNCC	6 cut(s)	280, 785, 786, 916, 1204, 1306
BspPI	GGATC	3 cut(s)	909, 922, 1488
BspT107I	GGYRCC	2 cut(s)	278, 1202
BspTNI	GGTCTC	2 cut(s)	1513, 1599
BsrFI	RCCGGY	1 cut(s)	1163
BsrI	ACTGG	3 cut(s)	289, 946, 1783
BssAI	RCCGGY	1 cut(s)	1163
BssECI	CCNNGG	6 cut(s)	391, 648, 918, 1335, 1523, 1598
BssMI	GATC	8 cut(s)	128, 253, 373, 914, 950, 1050, 1493, 1545
BssSI	CACGAG	2 cut(s)	983, 1137
BssT1I	CCWWGG	3 cut(s)	648, 918, 1598
Bst2BI	CACGAG	2 cut(s)	983, 1137
Bst2UI	CCWGG	3 cut(s)	393, 502, 1247
Bst4CI	ACNGT	7 cut(s)	307, 1108, 1278, 1309, 1457, 1524, 1738
Bst6I	CTCTTC	1 cut(s)	1487
BstBAI	YACGTR	1 cut(s)	964
BstC8I	GCNNGC	3 cut(s)	324, 1165, 1385
BstDEI	CTNAG	9 cut(s)	318, 506, 605, 705, 793, 852, 1072, 1330, 1672
BstDSI	CCRYGG	3 cut(s)	648, 1335, 1523
BstENI	CCTNNNNNAGG	2 cut(s)	796, 1675
BstF5I	GGATG	5 cut(s)	131, 592, 862, 1356, 1573
BstFNI	CGCG	1 cut(s)	399
BstHHI	GCGC	1 cut(s)	176
BstKTI	GATC	8 cut(s)	131, 256, 376, 917, 953, 1053, 1496, 1548
BstMAI	GTCTC	6 cut(s)	13, 670, 693, 1499, 1513, 1599
BstMBI	GATC	8 cut(s)	128, 253, 373, 914, 950, 1050, 1493, 1545
BstMWI	GCNNNNNNNGC	5 cut(s)	323, 731, 848, 1109, 1419
BstNI	CCWGG	3 cut(s)	393, 502, 1247
BstNSI	RCATGY	1 cut(s)	31
BstPAI	GACNNNNGTC	1 cut(s)	782
BstSCI	CCNGG	5 cut(s)	391, 500, 786, 1245, 1485
BstSFI	CTRYAG	1 cut(s)	435
BstSNI	TACGTA	1 cut(s)	964
BstUI	CGCG	1 cut(s)	399
BstV1I	GCAGC	6 cut(s)	524, 891, 1099, 1431, 1540, 1755
BstV2I	GAAGAC	1 cut(s)	754
BstX2I	RGATCY	3 cut(s)	914, 950, 1493
BstYI	RGATCY	3 cut(s)	914, 950, 1493
Bsu36I	CCTNAGG	1 cut(s)	1672
BsuRI	GGCC	3 cut(s)	791, 1167, 1413
BtgI	CCRYGG	3 cut(s)	648, 1335, 1523
BtgZI	GCGATG	1 cut(s)	1117
BtrI	CACGTC	1 cut(s)	780
BtsCI	GGATG	5 cut(s)	131, 592, 862, 1356, 1573
BtsI	GCAGTG	2 cut(s)	667, 897
BtsIMutI	CAGTG	4 cut(s)	667, 702, 897, 1776
Cac8I	GCNNGC	3 cut(s)	324, 1165, 1385
CaiI	CAGNNNCTG	1 cut(s)	842
CciI	TCATGA	2 cut(s)	376, 456
CfoI	GCGC	1 cut(s)	176
Cfr10I	RCCGGY	1 cut(s)	1163
Cfr13I	GGNCC	3 cut(s)	295, 784, 1166
CseI	GACGC	1 cut(s)	877
Csp6I	GTAC	5 cut(s)	933, 939, 988, 1630, 1642
CviAII	CATG	8 cut(s)	28, 377, 457, 649, 1552, 1564, 1645, 1700
CviQI	GTAC	5 cut(s)	933, 939, 988, 1630, 1642
DdeI	CTNAG	9 cut(s)	318, 506, 605, 705, 793, 852, 1072, 1330, 1672
DpnI	GATC	8 cut(s)	130, 255, 375, 916, 952, 1052, 1495, 1547
DpnII	GATC	8 cut(s)	128, 253, 373, 914, 950, 1050, 1493, 1545
DrdI	GACNNNNNNGTC	2 cut(s)	469, 1214
DriI	GACNNNNNGTC	1 cut(s)	706
DseDI	GACNNNNNNGTC	2 cut(s)	469, 1214
Eam1104I	CTCTTC	1 cut(s)	1487
Eam1105I	GACNNNNNGTC	1 cut(s)	706
EarI	CTCTTC	1 cut(s)	1487
Ecl136II	GAGCTC	1 cut(s)	332
Eco105I	TACGTA	1 cut(s)	964
Eco130I	CCWWGG	3 cut(s)	648, 918, 1598
Eco24I	GRGCYC	2 cut(s)	334, 1389
Eco31I	GGTCTC	2 cut(s)	1513, 1599
Eco32I	GATATC	1 cut(s)	1697
Eco47I	GGWCC	2 cut(s)	295, 784
Eco53kI	GAGCTC	1 cut(s)	332
Eco81I	CCTNAGG	1 cut(s)	1672
Eco88I	CYCGRG	3 cut(s)	327, 548, 696
EcoICRI	GAGCTC	1 cut(s)	332
EcoNI	CCTNNNNNAGG	2 cut(s)	796, 1675
EcoO109I	RGGNCCY	1 cut(s)	784
EcoRI	GAATTC	1 cut(s)	1648
EcoRII	CCWGG	3 cut(s)	391, 500, 1245
EcoRV	GATATC	1 cut(s)	1697
EcoT14I	CCWWGG	3 cut(s)	648, 918, 1598
EcoT22I	ATGCAT	1 cut(s)	1705
EcoT38I	GRGCYC	2 cut(s)	334, 1389
ErhI	CCWWGG	3 cut(s)	648, 918, 1598
Esp3I	CGTCTC	1 cut(s)	1499
FaeI	CATG	8 cut(s)	31, 380, 460, 652, 1555, 1567, 1648, 1703
FaqI	GGGAC	3 cut(s)	770, 880, 988
FatI	CATG	8 cut(s)	27, 376, 456, 648, 1551, 1563, 1644, 1699
FauI	CCCGC	1 cut(s)	1376
FbaI	TGATCA	1 cut(s)	253
FblI	GTMKAC	1 cut(s)	1311
Fnu4HI	GCNGC	8 cut(s)	513, 905, 1113, 1420, 1467, 1470, 1529, 1769
FokI	GGATG	5 cut(s)	118, 599, 869, 1343, 1580
FriOI	GRGCYC	2 cut(s)	334, 1389
Fsp4HI	GCNGC	8 cut(s)	513, 905, 1113, 1420, 1467, 1470, 1529, 1769
FspBI	CTAG	7 cut(s)	126, 272, 539, 629, 675, 809, 936
FspI	TGCGCA	1 cut(s)	175
GlaI	GCGC	1 cut(s)	175
GluI	GCNGC	8 cut(s)	513, 905, 1113, 1420, 1467, 1470, 1529, 1769
GsaI	CCCAGC	1 cut(s)	975
GsuI	CTGGAG	1 cut(s)	1363
HaeIII	GGCC	3 cut(s)	791, 1167, 1413
HapII	CCGG	4 cut(s)	135, 788, 1164, 1487
HgaI	GACGC	1 cut(s)	877
HhaI	GCGC	1 cut(s)	176
Hin1II	CATG	8 cut(s)	31, 380, 460, 652, 1555, 1567, 1648, 1703
Hin6I	GCGC	1 cut(s)	174
HinP1I	GCGC	1 cut(s)	174
HincII	GTYRAC	1 cut(s)	1312
HindII	GTYRAC	1 cut(s)	1312
HindIII	AAGCTT	3 cut(s)	90, 803, 813
HinfI	GANTC	5 cut(s)	203, 707, 1197, 1571, 1759
HpaII	CCGG	4 cut(s)	135, 788, 1164, 1487
HphI	GGTGA	5 cut(s)	179, 896, 1043, 1133, 1501
Hpy166II	GTNNAC	6 cut(s)	385, 496, 1156, 1222, 1312, 1716
Hpy188I	TCNGA	9 cut(s)	253, 606, 685, 763, 777, 796, 1075, 1431, 1477
Hpy8I	GTNNAC	6 cut(s)	385, 496, 1156, 1222, 1312, 1716
Hpy99I	CGWCG	3 cut(s)	494, 781, 1313
HpyAV	CCTTC	7 cut(s)	108, 269, 679, 889, 1179, 1671, 1782
HpyCH4III	ACNGT	7 cut(s)	307, 1108, 1278, 1309, 1457, 1524, 1738
HpyCH4IV	ACGT	6 cut(s)	492, 779, 963, 1017, 1508, 1712
HpyCH4V	TGCA	6 cut(s)	512, 734, 1353, 1555, 1616, 1703
HpyF10VI	GCNNNNNNNGC	5 cut(s)	323, 731, 848, 1109, 1419
HpyF3I	CTNAG	9 cut(s)	318, 506, 605, 705, 793, 852, 1072, 1330, 1672
HpySE526I	ACGT	6 cut(s)	492, 779, 963, 1017, 1508, 1712
Hsp92II	CATG	8 cut(s)	31, 380, 460, 652, 1555, 1567, 1648, 1703
HspAI	GCGC	1 cut(s)	174
KflI	GGGWCCC	1 cut(s)	784
Kpn2I	TCCGGA	1 cut(s)	134
KroI	GCCGGC	1 cut(s)	1163
KroNI	GCCGGC	1 cut(s)	1165
Ksp22I	TGATCA	1 cut(s)	253
Kzo9I	GATC	8 cut(s)	128, 253, 373, 914, 950, 1050, 1493, 1545
LmnI	GCTCC	2 cut(s)	227, 685
Lsp1109I	GCAGC	6 cut(s)	524, 891, 1099, 1431, 1540, 1755
LweI	GCATC	7 cut(s)	421, 767, 829, 1150, 1481, 1673, 1712
MaeI	CTAG	7 cut(s)	126, 272, 539, 629, 675, 809, 936
MaeII	ACGT	6 cut(s)	492, 779, 963, 1017, 1508, 1712
MaeIII	GTNAC	2 cut(s)	589, 1399
MalI	GATC	8 cut(s)	130, 255, 375, 916, 952, 1052, 1495, 1547
MboI	GATC	8 cut(s)	128, 253, 373, 914, 950, 1050, 1493, 1545
MboII	GAAGA	4 cut(s)	58, 759, 1312, 1474
MflI	RGATCY	3 cut(s)	914, 950, 1493
MhlI	GDGCHC	2 cut(s)	334, 1389
MluCI	AATT	6 cut(s)	36, 57, 119, 909, 1648, 1803
MlyI	GAGTC	3 cut(s)	716, 1206, 1565
MmeI	TCCRAC	1 cut(s)	800
Mph1103I	ATGCAT	1 cut(s)	1705
MroI	TCCGGA	1 cut(s)	134
MroNI	GCCGGC	1 cut(s)	1163
MseI	TTAA	2 cut(s)	1620, 1683
MslI	CAYNNNNRTG	2 cut(s)	26, 1781
MspA1I	CMGCKG	1 cut(s)	1383
MspI	CCGG	4 cut(s)	135, 788, 1164, 1487
MspR9I	CCNGG	5 cut(s)	393, 502, 788, 1247, 1487
Mva1269I	GAATGC	1 cut(s)	1730
MvaI	CCWGG	3 cut(s)	393, 502, 1247
MvnI	CGCG	1 cut(s)	399
MwoI	GCNNNNNNNGC	5 cut(s)	323, 731, 848, 1109, 1419
NaeI	GCCGGC	1 cut(s)	1165
NciI	CCSGG	2 cut(s)	788, 1487
NcoI	CCATGG	1 cut(s)	648
NdeII	GATC	8 cut(s)	128, 253, 373, 914, 950, 1050, 1493, 1545
NgoMIV	GCCGGC	1 cut(s)	1163
NlaIII	CATG	8 cut(s)	31, 380, 460, 652, 1555, 1567, 1648, 1703
NlaIV	GGNNCC	6 cut(s)	280, 785, 786, 916, 1204, 1306
NmuCI	GTSAC	2 cut(s)	589, 1399
NsbI	TGCGCA	1 cut(s)	175
NsiI	ATGCAT	1 cut(s)	1705
NspI	RCATGY	1 cut(s)	31
OliI	CACNNNNGTG	1 cut(s)	1781
PaeR7I	CTCGAG	3 cut(s)	327, 548, 696
PagI	TCATGA	2 cut(s)	376, 456
PcsI	WCGNNNNNNNCGW	1 cut(s)	489
PctI	GAATGC	1 cut(s)	1730
PdiI	GCCGGC	1 cut(s)	1165
PfeI	GAWTC	2 cut(s)	203, 1759
PflFI	GACNNNGTC	1 cut(s)	452
PkrI	GCNGC	8 cut(s)	514, 906, 1114, 1421, 1468, 1471, 1530, 1770
PleI	GAGTC	3 cut(s)	715, 1205, 1565
PpsI	GAGTC	3 cut(s)	715, 1205, 1565
Ppu21I	YACGTR	1 cut(s)	964
PpuMI	RGGWCCY	1 cut(s)	784
PshAI	GACNNNNGTC	1 cut(s)	782
PsiI	TTATAA	1 cut(s)	1095
Psp124BI	GAGCTC	1 cut(s)	334
Psp5II	RGGWCCY	1 cut(s)	784
Psp6I	CCWGG	3 cut(s)	391, 500, 1245
PspFI	CCCAGC	1 cut(s)	971
PspGI	CCWGG	3 cut(s)	391, 500, 1245
PspN4I	GGNNCC	6 cut(s)	280, 785, 786, 916, 1204, 1306
PspPI	GGNCC	3 cut(s)	295, 784, 1166
PspPPI	RGGWCCY	1 cut(s)	784
PspXI	VCTCGAGB	1 cut(s)	327
PstNI	CAGNNNCTG	1 cut(s)	842
PsuI	RGATCY	3 cut(s)	914, 950, 1493
PsyI	GACNNNGTC	1 cut(s)	452
RsaI	GTAC	5 cut(s)	934, 940, 989, 1631, 1643
RsaNI	GTAC	5 cut(s)	933, 939, 988, 1630, 1642
RseI	CAYNNNNRTG	2 cut(s)	26, 1781
SacI	GAGCTC	1 cut(s)	334
SalI	GTCGAC	1 cut(s)	1310
SaqAI	TTAA	2 cut(s)	1620, 1683
SatI	GCNGC	8 cut(s)	513, 905, 1113, 1420, 1467, 1470, 1529, 1769
Sau3AI	GATC	8 cut(s)	128, 253, 373, 914, 950, 1050, 1493, 1545
Sau96I	GGNCC	3 cut(s)	295, 784, 1166
ScaI	AGTACT	1 cut(s)	940
SchI	GAGTC	3 cut(s)	716, 1206, 1565
ScrFI	CCNGG	5 cut(s)	393, 502, 788, 1247, 1487
SduI	GDGCHC	2 cut(s)	334, 1389
SfaNI	GCATC	7 cut(s)	421, 767, 829, 1150, 1481, 1673, 1712
SfcI	CTRYAG	1 cut(s)	435
Sfr274I	CTCGAG	3 cut(s)	327, 548, 696
SinI	GGWCC	2 cut(s)	295, 784
SlaI	CTCGAG	3 cut(s)	327, 548, 696
SmiMI	CAYNNNNRTG	2 cut(s)	26, 1781
SmlI	CTYRAG	8 cut(s)	70, 327, 416, 548, 696, 1388, 1407, 1719
SmoI	CTYRAG	8 cut(s)	70, 327, 416, 548, 696, 1388, 1407, 1719
SnaBI	TACGTA	1 cut(s)	964
SpeI	ACTAGT	1 cut(s)	935
Sse9I	AATT	6 cut(s)	36, 57, 119, 909, 1648, 1803
SsiI	CCGC	7 cut(s)	50, 361, 399, 1373, 1383, 1466, 1469
SspMI	CTAG	7 cut(s)	126, 272, 539, 629, 675, 809, 936
SstI	GAGCTC	1 cut(s)	334
StyD4I	CCNGG	5 cut(s)	391, 500, 786, 1245, 1485
StyI	CCWWGG	3 cut(s)	648, 918, 1598
TaaI	ACNGT	7 cut(s)	307, 1108, 1278, 1309, 1457, 1524, 1738
TaiI	ACGT	6 cut(s)	495, 782, 966, 1020, 1511, 1715
TaqII	GACCGA	1 cut(s)	283
TasI	AATT	6 cut(s)	36, 57, 119, 909, 1648, 1803
TatI	WGTACW	4 cut(s)	938, 987, 1629, 1641
TauI	GCSGC	2 cut(s)	1469, 1472
TfiI	GAWTC	2 cut(s)	203, 1759
Tru1I	TTAA	2 cut(s)	1620, 1683
Tru9I	TTAA	2 cut(s)	1620, 1683
TscAI	CASTG	4 cut(s)	667, 709, 897, 1783
TseFI	GTSAC	2 cut(s)	589, 1399
TseI	GCWGC	6 cut(s)	512, 904, 1112, 1419, 1528, 1768
Tsp45I	GTSAC	2 cut(s)	589, 1399
TspDTI	ATGAA	5 cut(s)	1120, 1306, 1552, 1661, 1738
TspRI	CASTG	4 cut(s)	667, 709, 897, 1783
Tth111I	GACNNNGTC	1 cut(s)	452
VpaK11BI	GGWCC	2 cut(s)	295, 784
XagI	CCTNNNNNAGG	2 cut(s)	796, 1675
XapI	RAATTY	3 cut(s)	57, 119, 1648
XbaI	TCTAGA	4 cut(s)	125, 271, 538, 808
XceI	RCATGY	1 cut(s)	31
XhoI	CTCGAG	3 cut(s)	327, 548, 696
XmiI	GTMKAC	1 cut(s)	1311
XspI	CTAG	7 cut(s)	126, 272, 539, 629, 675, 809, 936
ZrmI	AGTACT	1 cut(s)	940
Zsp2I	ATGCAT	1 cut(s)	1705

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.