RchiOBHm_Chr4g0410411

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009176
Physical Location & Seq
Reverse (-)
34154059 .. 34156304
2246 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 732 bp
ATGCCACTAAGTATCAAGTGGCATGAACAACTACATGGCTTTACTTCATTCCCATCCCCTGCTACTCCAATCTTCAATACCAGGAAGAAAGTTGAAGTGATAAGGGTGTTAGCTAAAGCAGCTAACTTGGGTTCTGAATCAAATGCAAAGAAAGCAAATTTGTGTGCTGCAAAGAAGGAAAGAATTAAGCTGCCAAGTTTTGATGGTAGCTTAGGGCGCAAGAATTTTCACATTAGAGAGTTTCTAAGCCACCCATCAGGAACTGAAGCCATGCTCAATACTAGAGCACTGGAAAATTTTGAACCCCTTAGCACTGATACTTACAGGTGCACTTTGCCAAAGCTTAAACTTTTGAATTTTGAAGCAGCCCCTGTGGTGGATTTAAGAGTGAACCCAACCAATGAGGATTGTATGGTTGAGATGCTCTCTTGCAAGTTTGAGGGTTCAGAATCTGTGGAACGCCAAAACAGCCGTTTTTCAGCATTGATGACAAATCACATGTCGTGGGGGACAGACGACTCTGAATCATTTTTGGAAGTTGATGTGAAGTTAAATCTCACCCTCGAGATCTATACGCGCCCTTTTACTATGATGCCGGTATCAGCTGTTGAGCGTCCGGGAAACTTAATGATGCAAGCATTACTGGATAGGCTGGTCCCACTGCTTCTGCAGCAACTCCTACAAGATTATGACAAATGGGTTCATAAGCAATCAGAAGATGATCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

243

Amino Acids

27.61

Weight (kDa)

6.32

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 78 - 234 3.3e-38 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 577
AcsI RAATTY 4 cut(s) 157, 223, 295, 355
AcuI CTGAAG 1 cut(s) 285
AfiI CCNNNNNNNGG 1 cut(s) 376
AflIII ACRYGT 1 cut(s) 498
AgsI TTSAA 5 cut(s) 76, 95, 302, 355, 362
AjnI CCWGG 1 cut(s) 80
AluBI AGCT 6 cut(s) 113, 122, 190, 210, 343, 605
AluI AGCT 6 cut(s) 113, 122, 190, 210, 343, 605
Alw21I GWGCWC 2 cut(s) 289, 332
Alw44I GTGCAC 1 cut(s) 328
AlwNI CAGNNNCTG 3 cut(s) 263, 371, 452
Ama87I CYCGRG 1 cut(s) 563
ApaLI GTGCAC 1 cut(s) 328
ApeKI GCWGC 5 cut(s) 119, 167, 190, 365, 670
ApoI RAATTY 4 cut(s) 157, 223, 295, 355
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
AspLEI GCGC 2 cut(s) 219, 579
AspS9I GGNCC 1 cut(s) 655
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 1 cut(s) 550
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 1 cut(s) 655
BaeGI GKGCMC 1 cut(s) 332
Bbv12I GWGCWC 2 cut(s) 289, 332
BbvI GCAGC 5 cut(s) 131, 154, 177, 377, 682
BccI CCATC 3 cut(s) 61, 197, 262
BceAI ACGGC 1 cut(s) 456
BciT130I CCWGG 1 cut(s) 82
BcnI CCSGG 1 cut(s) 618
BfaI CTAG 1 cut(s) 282
BfmI CTRYAG 1 cut(s) 668
BglII AGATCT 1 cut(s) 567
BisI GCNGC 5 cut(s) 120, 168, 191, 366, 671
BlsI GCNGC 5 cut(s) 121, 169, 192, 367, 672
Bme1390I CCNGG 2 cut(s) 82, 618
Bme18I GGWCC 1 cut(s) 655
BmeT110I CYCGRG 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 655
BmiI GGNNCC 1 cut(s) 657
BmrFI CCNGG 2 cut(s) 82, 618
BmsI GCATC 3 cut(s) 411, 582, 621
BplI GAGNNNNNCTC 2 cut(s) 410, 442
Bpu10I CCTNAGC 2 cut(s) 211, 308
BpuMI CCSGG 1 cut(s) 618
Bsc4I CCNNNNNNNGG 1 cut(s) 376
Bse118I RCCGGY 1 cut(s) 595
Bse1I ACTGG 2 cut(s) 294, 648
BseBI CCWGG 1 cut(s) 82
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 1 cut(s) 376
BseNI ACTGG 2 cut(s) 294, 648
BseSI GKGCMC 1 cut(s) 332
BseXI GCAGC 5 cut(s) 131, 154, 177, 377, 682
Bsh1236I CGCG 1 cut(s) 577
BsiHKAI GWGCWC 2 cut(s) 289, 332
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 2 cut(s) 596, 617
BslFI GGGAC 2 cut(s) 523, 641
BslI CCNNNNNNNGG 1 cut(s) 376
BsmFI GGGAC 2 cut(s) 523, 641
BsoBI CYCGRG 1 cut(s) 563
Bsp1286I GDGCHC 2 cut(s) 289, 332
Bsp143I GATC 2 cut(s) 567, 721
BspFNI CGCG 1 cut(s) 577
BspLI GGNNCC 1 cut(s) 657
BspMAI CTGCAG 1 cut(s) 672
BsrFI RCCGGY 1 cut(s) 595
BsrI ACTGG 2 cut(s) 294, 648
BssAI RCCGGY 1 cut(s) 595
BssMI GATC 2 cut(s) 567, 721
Bst2UI CCWGG 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 636
BstDEI CTNAG 4 cut(s) 8, 211, 245, 308
BstF5I GGATG 1 cut(s) 53
BstFNI CGCG 1 cut(s) 577
BstHHI GCGC 2 cut(s) 219, 579
BstKTI GATC 2 cut(s) 570, 724
BstMBI GATC 2 cut(s) 567, 721
BstMWI GCNNNNNNNGC 5 cut(s) 119, 152, 216, 468, 670
BstNI CCWGG 1 cut(s) 82
BstNSI RCATGY 1 cut(s) 502
BstSCI CCNGG 2 cut(s) 80, 616
BstSFI CTRYAG 1 cut(s) 668
BstSLI GKGCMC 1 cut(s) 332
BstUI CGCG 1 cut(s) 577
BstV1I GCAGC 5 cut(s) 131, 154, 177, 377, 682
BstX2I RGATCY 1 cut(s) 567
BstYI RGATCY 1 cut(s) 567
BtsCI GGATG 1 cut(s) 53
BtsI GCAGTG 1 cut(s) 659
BtsIMutI CAGTG 3 cut(s) 287, 312, 659
Cac8I GCNNGC 1 cut(s) 636
CaiI CAGNNNCTG 3 cut(s) 263, 371, 452
CfoI GCGC 2 cut(s) 219, 579
Cfr10I RCCGGY 1 cut(s) 595
Cfr13I GGNCC 1 cut(s) 655
CseI GACGC 1 cut(s) 602
CviAII CATG 5 cut(s) 23, 35, 271, 499, 729
DdeI CTNAG 4 cut(s) 8, 211, 245, 308
DpnI GATC 2 cut(s) 569, 723
DpnII GATC 2 cut(s) 567, 721
Eco47I GGWCC 1 cut(s) 655
Eco57I CTGAAG 1 cut(s) 285
Eco88I CYCGRG 1 cut(s) 563
EcoRII CCWGG 1 cut(s) 80
FaeI CATG 5 cut(s) 26, 38, 274, 502, 732
FalI AAGNNNNNCTT 1 cut(s) 708
FaqI GGGAC 2 cut(s) 523, 641
FatI CATG 5 cut(s) 22, 34, 270, 498, 728
Fnu4HI GCNGC 5 cut(s) 120, 168, 191, 366, 671
FokI GGATG 1 cut(s) 40
Fsp4HI GCNGC 5 cut(s) 120, 168, 191, 366, 671
FspBI CTAG 1 cut(s) 282
GlaI GCGC 2 cut(s) 218, 578
GluI GCNGC 5 cut(s) 120, 168, 191, 366, 671
HapII CCGG 2 cut(s) 596, 617
HgaI GACGC 1 cut(s) 602
HhaI GCGC 2 cut(s) 219, 579
Hin1II CATG 5 cut(s) 26, 38, 274, 502, 732
Hin6I GCGC 2 cut(s) 217, 577
HinP1I GCGC 2 cut(s) 217, 577
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 4 cut(s) 137, 449, 518, 524
HpaII CCGG 2 cut(s) 596, 617
HphI GGTGA 1 cut(s) 550
Hpy166II GTNNAC 2 cut(s) 330, 391
Hpy188I TCNGA 4 cut(s) 136, 448, 523, 715
Hpy188III TCNNGA 2 cut(s) 258, 565
Hpy8I GTNNAC 2 cut(s) 330, 391
HpyAV CCTTC 1 cut(s) 169
HpyCH4V TGCA 6 cut(s) 146, 170, 330, 432, 634, 670
HpyF10VI GCNNNNNNNGC 5 cut(s) 119, 152, 216, 468, 670
HpyF3I CTNAG 4 cut(s) 8, 211, 245, 308
Hsp92II CATG 5 cut(s) 26, 38, 274, 502, 732
HspAI GCGC 2 cut(s) 217, 577
Kzo9I GATC 2 cut(s) 567, 721
Lsp1109I GCAGC 5 cut(s) 131, 154, 177, 377, 682
LweI GCATC 3 cut(s) 411, 582, 621
MaeI CTAG 1 cut(s) 282
MalI GATC 2 cut(s) 569, 723
MboI GATC 2 cut(s) 567, 721
MboII GAAGA 4 cut(s) 64, 97, 716, 728
MflI RGATCY 1 cut(s) 567
MhlI GDGCHC 2 cut(s) 289, 332
MluCI AATT 5 cut(s) 157, 183, 223, 295, 355
MlyI GAGTC 1 cut(s) 512
MnlI CCTC 3 cut(s) 397, 433, 572
MseI TTAA 5 cut(s) 186, 345, 383, 551, 626
MspA1I CMGCKG 1 cut(s) 605
MspI CCGG 2 cut(s) 596, 617
MspR9I CCNGG 2 cut(s) 82, 618
MvaI CCWGG 1 cut(s) 82
MvnI CGCG 1 cut(s) 577
MwoI GCNNNNNNNGC 5 cut(s) 119, 152, 216, 468, 670
NciI CCSGG 1 cut(s) 618
NdeII GATC 2 cut(s) 567, 721
NlaIII CATG 5 cut(s) 26, 38, 274, 502, 732
NlaIV GGNNCC 1 cut(s) 657
NspI RCATGY 1 cut(s) 502
PaeR7I CTCGAG 1 cut(s) 563
PciI ACATGT 1 cut(s) 498
PfeI GAWTC 3 cut(s) 137, 449, 524
PfoI TCCNGGA 1 cut(s) 616
PkrI GCNGC 5 cut(s) 121, 169, 192, 367, 672
PleI GAGTC 1 cut(s) 512
PpsI GAGTC 1 cut(s) 512
PscI ACATGT 1 cut(s) 498
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspN4I GGNNCC 1 cut(s) 657
PspPI GGNCC 1 cut(s) 655
PstI CTGCAG 1 cut(s) 672
PstNI CAGNNNCTG 3 cut(s) 263, 371, 452
PsuI RGATCY 1 cut(s) 567
PvuII CAGCTG 1 cut(s) 605
SaqAI TTAA 5 cut(s) 186, 345, 383, 551, 626
SatI GCNGC 5 cut(s) 120, 168, 191, 366, 671
Sau3AI GATC 2 cut(s) 567, 721
Sau96I GGNCC 1 cut(s) 655
SchI GAGTC 1 cut(s) 512
ScrFI CCNGG 2 cut(s) 82, 618
SduI GDGCHC 2 cut(s) 289, 332
SetI ASST 7 cut(s) 115, 124, 192, 212, 329, 345, 607
SfaNI GCATC 3 cut(s) 411, 582, 621
SfcI CTRYAG 1 cut(s) 668
Sfr274I CTCGAG 1 cut(s) 563
SinI GGWCC 1 cut(s) 655
SlaI CTCGAG 1 cut(s) 563
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
Sse9I AATT 5 cut(s) 157, 183, 223, 295, 355
SspMI CTAG 1 cut(s) 282
StyD4I CCNGG 2 cut(s) 80, 616
TaqI TCGA 1 cut(s) 564
TasI AATT 5 cut(s) 157, 183, 223, 295, 355
TfiI GAWTC 3 cut(s) 137, 449, 524
Tru1I TTAA 5 cut(s) 186, 345, 383, 551, 626
Tru9I TTAA 5 cut(s) 186, 345, 383, 551, 626
TscAI CASTG 3 cut(s) 294, 319, 666
TseI GCWGC 5 cut(s) 119, 167, 190, 365, 670
TspDTI ATGAA 3 cut(s) 36, 39, 692
TspRI CASTG 3 cut(s) 294, 319, 666
VneI GTGCAC 1 cut(s) 328
VpaK11BI GGWCC 1 cut(s) 655
XapI RAATTY 4 cut(s) 157, 223, 295, 355
XceI RCATGY 1 cut(s) 502
XhoI CTCGAG 1 cut(s) 563
XspI CTAG 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.