RchiOBHm_Chr4g0413601

Werner Syndrome-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003549
Physical Location & Seq
Forward (+)
37866204 .. 37872624
6421 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 981 bp
ATGGAGCACTCGCAATCTCAAGATGACAGCAAAGGCAAAGCCATCAGCACCACCTTATCGGAATCCGAATGGGATGAGCCCTTCACAGAGGAAGACCTCCAAGCCATTGAAGCCGCATTCGAAGCCGCCGCGACTTCTTCTCTCTCCAAAAAGCGACGCTCCAATCCAGACGACGAAGTCACGGACCAGTCCCAGAGCGCCCGGCGCCGACTGCCGAGTTCAGTTCTCGCTCTCCAGCATCCAAACGCCTTCGCGCTCTCGCCTTGTCGTCAAGCTGCCAATATAAGGATGAGATATCCTGTGTTGAAGTTTGGAGGTCGGGTTACATATAGCAGGACTGCAGTTGAAGTGGAGAAAGCTGCCATGGAGATTTTAAAGACTGTGAAAGCAAAGACAAACGAAATGGGTCAAAATGCAGTTGGATTTGATATTGAGTGGAAACCCACATTCCAAAGAGGTGTTCCACCTGGGAAGGTTGCAGTTCTGCAGATATGTGGAGACACAAGTTGTTGCCATGTGATGCATATCATTCATTCTGGAATTCCTCGAAGTCTGCAGTTACTTCTTGAAAGTTCTTCGATTTTGAAGGTTGGAGCTGGTATCGCTAATGATGCTGTTAAGGTTTTCAAGGATTATAATGTATCTATTAAAGCTGTGGAGGATCTTTCATATCTGGCTAATCATAAGCTTGGAGATTCACAAAATTGGGGTCTTGCATCCCTAACTGAGAAGCTCATTTCTAAACAGCTTCTAAAGCCCAAAAAAATTCGATTGGGTAACTGGGAGACCAAATATTTATCAAAAGAGCAGCTGCAGTATGCTGCCACTGATGCATTTGCATCATGGTATCTTCATGAGGTGCTAAGGAGCCTCCCTGATGCTGAAAAACTTGAAAAAGTTACAGCAGACAACCAGAGTGAGGGACAAGCCGTATCATGTAATTACTTTCAGACTGAGGAACCACAAGCTGCATCATTGTAA

Protein Analysis

326

Amino Acids

36.08

Weight (kDa)

6.41

Isoelectric Point (pI)

57.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 121 - 289 1.7e-21 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 636
AasI GACNNNNNNGTC 1 cut(s) 176
AccB1I GGYRCC 1 cut(s) 204
AccII CGCG 2 cut(s) 131, 254
AciI CCGC 3 cut(s) 114, 126, 129
AclWI GGATC 1 cut(s) 669
AcsI RAATTY 2 cut(s) 540, 765
AcyI GRCGYC 1 cut(s) 205
AfiI CCNNNNNNNGG 2 cut(s) 285, 919
AgsI TTSAA 7 cut(s) 110, 307, 347, 569, 586, 628, 893
AjnI CCWGG 1 cut(s) 466
AjuI GAANNNNNNNTTGG 2 cut(s) 444, 476
AluBI AGCT 9 cut(s) 275, 359, 596, 653, 688, 733, 748, 811, 968
AluI AGCT 9 cut(s) 275, 359, 596, 653, 688, 733, 748, 811, 968
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 2 cut(s) 492, 779
AlwI GGATC 1 cut(s) 669
ApeKI GCWGC 6 cut(s) 275, 359, 808, 811, 821, 968
ApoI RAATTY 2 cut(s) 540, 765
ArsI GACNNNNNNTTYG 2 cut(s) 694, 726
AspLEI GCGC 3 cut(s) 200, 207, 256
AspS9I GGNCC 1 cut(s) 184
AsuC2I CCSGG 1 cut(s) 202
AsuII TTCGAA 1 cut(s) 120
AvaII GGWCC 1 cut(s) 184
BanI GGYRCC 1 cut(s) 204
BanII GRGCYC 1 cut(s) 81
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 6 cut(s) 262, 346, 798, 808, 820, 955
BccI CCATC 1 cut(s) 50
BceAI ACGGC 1 cut(s) 914
BciT130I CCWGG 1 cut(s) 468
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 2 cut(s) 492, 779
BfmI CTRYAG 4 cut(s) 339, 485, 554, 812
BfoI RGCGCY 2 cut(s) 201, 208
BisI GCNGC 9 cut(s) 114, 126, 129, 276, 360, 809, 812, 822, 969
BlsI GCNGC 9 cut(s) 115, 127, 130, 277, 361, 810, 813, 823, 970
Bme1390I CCNGG 2 cut(s) 202, 468
Bme18I GGWCC 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 184
BmiI GGNNCC 3 cut(s) 206, 869, 960
BmrFI CCNGG 2 cut(s) 202, 468
BmrI ACTGGG 1 cut(s) 790
BmsI GCATC 7 cut(s) 247, 510, 601, 725, 820, 848, 868
BmuI ACTGGG 1 cut(s) 790
BpiI GAAGAC 1 cut(s) 99
BpmI CTGGAG 1 cut(s) 218
Bpu10I CCTNAGC 1 cut(s) 863
Bpu14I TTCGAA 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 202
BsaBI GATNNNNATC 1 cut(s) 524
BsaHI GRCGYC 1 cut(s) 205
BsaI GGTCTC 1 cut(s) 779
BsaJI CCNNGG 2 cut(s) 363, 467
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bsc4I CCNNNNNNNGG 2 cut(s) 285, 919
Bse1I ACTGG 2 cut(s) 187, 785
Bse8I GATNNNNATC 1 cut(s) 524
BseBI CCWGG 1 cut(s) 468
BseDI CCNNGG 2 cut(s) 363, 467
BseGI GGATG 4 cut(s) 79, 238, 294, 716
BseJI GATNNNNATC 1 cut(s) 524
BseLI CCNNNNNNNGG 2 cut(s) 285, 919
BseMII CTCAG 2 cut(s) 717, 945
BseNI ACTGG 2 cut(s) 187, 785
BseXI GCAGC 6 cut(s) 262, 346, 798, 808, 820, 955
Bsh1236I CGCG 2 cut(s) 131, 254
BshNI GGYRCC 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 1 cut(s) 202
BslFI GGGAC 2 cut(s) 175, 936
BslI CCNNNNNNNGG 2 cut(s) 285, 919
BsmAI GTCTC 2 cut(s) 492, 779
BsmFI GGGAC 2 cut(s) 175, 936
BsmI GAATGC 1 cut(s) 116
Bso31I GGTCTC 1 cut(s) 779
Bsp119I TTCGAA 1 cut(s) 120
Bsp1286I GDGCHC 2 cut(s) 9, 81
Bsp143I GATC 1 cut(s) 661
Bsp19I CCATGG 1 cut(s) 363
BspACI CCGC 3 cut(s) 114, 126, 129
BspCNI CTCAG 2 cut(s) 718, 946
BspFNI CGCG 2 cut(s) 131, 254
BspHI TCATGA 1 cut(s) 853
BspLI GGNNCC 3 cut(s) 206, 869, 960
BspMAI CTGCAG 4 cut(s) 343, 489, 558, 816
BspPI GGATC 1 cut(s) 669
BspT104I TTCGAA 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 204
BspTNI GGTCTC 1 cut(s) 779
BsrI ACTGG 2 cut(s) 187, 785
BssECI CCNNGG 2 cut(s) 363, 467
BssMI GATC 1 cut(s) 661
BssNI GRCGYC 1 cut(s) 205
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 1 cut(s) 382
BstACI GRCGYC 1 cut(s) 205
BstBI TTCGAA 1 cut(s) 120
BstDEI CTNAG 3 cut(s) 726, 863, 954
BstDSI CCRYGG 1 cut(s) 363
BstF5I GGATG 4 cut(s) 79, 238, 294, 716
BstFNI CGCG 2 cut(s) 131, 254
BstH2I RGCGCY 2 cut(s) 201, 208
BstHHI GCGC 3 cut(s) 200, 207, 256
BstKTI GATC 1 cut(s) 664
BstMAI GTCTC 2 cut(s) 492, 779
BstMBI GATC 1 cut(s) 661
BstMWI GCNNNNNNNGC 8 cut(s) 110, 122, 204, 211, 602, 611, 754, 830
BstNI CCWGG 1 cut(s) 468
BstSCI CCNGG 2 cut(s) 200, 466
BstSFI CTRYAG 4 cut(s) 339, 485, 554, 812
BstUI CGCG 2 cut(s) 131, 254
BstV1I GCAGC 6 cut(s) 262, 346, 798, 808, 820, 955
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 1 cut(s) 661
BstYI RGATCY 1 cut(s) 661
BtgI CCRYGG 1 cut(s) 363
BtsCI GGATG 4 cut(s) 79, 238, 294, 716
BtsIMutI CAGTG 1 cut(s) 825
CciI TCATGA 1 cut(s) 853
CfoI GCGC 3 cut(s) 200, 207, 256
Cfr13I GGNCC 1 cut(s) 184
CseI GACGC 1 cut(s) 165
CviAII CATG 5 cut(s) 364, 515, 843, 854, 936
DdeI CTNAG 3 cut(s) 726, 863, 954
DinI GGCGCC 1 cut(s) 206
DpnI GATC 1 cut(s) 663
DpnII GATC 1 cut(s) 661
DraI TTTAAA 1 cut(s) 375
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
Eco130I CCWWGG 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 81
Eco31I GGTCTC 1 cut(s) 779
Eco32I GATATC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 184
EcoRI GAATTC 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 466
EcoRV GATATC 1 cut(s) 296
EcoT14I CCWWGG 1 cut(s) 363
EcoT22I ATGCAT 2 cut(s) 525, 835
EcoT38I GRGCYC 1 cut(s) 81
EgeI GGCGCC 1 cut(s) 206
EheI GGCGCC 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 5 cut(s) 367, 518, 846, 857, 939
FaqI GGGAC 2 cut(s) 175, 936
FatI CATG 5 cut(s) 363, 514, 842, 853, 935
Fnu4HI GCNGC 9 cut(s) 114, 126, 129, 276, 360, 809, 812, 822, 969
FokI GGATG 4 cut(s) 86, 225, 301, 703
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 9 cut(s) 114, 126, 129, 276, 360, 809, 812, 822, 969
GlaI GCGC 3 cut(s) 199, 206, 255
GluI GCNGC 9 cut(s) 114, 126, 129, 276, 360, 809, 812, 822, 969
GsuI CTGGAG 1 cut(s) 218
HaeII RGCGCY 2 cut(s) 201, 208
HapII CCGG 1 cut(s) 202
HgaI GACGC 1 cut(s) 165
HhaI GCGC 3 cut(s) 200, 207, 256
Hin1I GRCGYC 1 cut(s) 205
Hin1II CATG 5 cut(s) 367, 518, 846, 857, 939
Hin6I GCGC 3 cut(s) 198, 205, 254
HinP1I GCGC 3 cut(s) 198, 205, 254
HindIII AAGCTT 1 cut(s) 686
HinfI GANTC 2 cut(s) 62, 695
HpaII CCGG 1 cut(s) 202
Hpy188I TCNGA 3 cut(s) 61, 67, 951
Hpy188III TCNNGA 5 cut(s) 20, 167, 537, 566, 854
Hpy99I CGWCG 2 cut(s) 159, 176
HpyAV CCTTC 4 cut(s) 91, 259, 466, 580
HpyCH4III ACNGT 1 cut(s) 382
HpyF10VI GCNNNNNNNGC 8 cut(s) 110, 122, 204, 211, 602, 611, 754, 830
HpyF3I CTNAG 3 cut(s) 726, 863, 954
Hsp92I GRCGYC 1 cut(s) 205
Hsp92II CATG 5 cut(s) 367, 518, 846, 857, 939
HspAI GCGC 3 cut(s) 198, 205, 254
KasI GGCGCC 1 cut(s) 204
Kzo9I GATC 1 cut(s) 661
LmnI GCTCC 4 cut(s) 4, 164, 593, 867
Lsp1109I GCAGC 6 cut(s) 262, 346, 798, 808, 820, 955
LweI GCATC 7 cut(s) 247, 510, 601, 725, 820, 848, 868
MaeIII GTNAC 5 cut(s) 178, 322, 558, 776, 898
MalI GATC 1 cut(s) 663
MboI GATC 1 cut(s) 661
MboII GAAGA 4 cut(s) 104, 129, 567, 842
MflI RGATCY 1 cut(s) 661
MhlI GDGCHC 2 cut(s) 9, 81
MluCI AATT 4 cut(s) 540, 703, 765, 940
Mly113I GGCGCC 1 cut(s) 205
MmeI TCCRAC 2 cut(s) 400, 571
Mph1103I ATGCAT 2 cut(s) 525, 835
MseI TTAA 3 cut(s) 374, 618, 648
MspA1I CMGCKG 1 cut(s) 811
MspI CCGG 1 cut(s) 202
MspR9I CCNGG 2 cut(s) 202, 468
Mva1269I GAATGC 1 cut(s) 116
MvaI CCWGG 1 cut(s) 468
MvnI CGCG 2 cut(s) 131, 254
MwoI GCNNNNNNNGC 8 cut(s) 110, 122, 204, 211, 602, 611, 754, 830
NarI GGCGCC 1 cut(s) 205
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 363
NdeII GATC 1 cut(s) 661
NlaIII CATG 5 cut(s) 367, 518, 846, 857, 939
NlaIV GGNNCC 3 cut(s) 206, 869, 960
NmeAIII GCCGAG 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 178
NsiI ATGCAT 2 cut(s) 525, 835
NspV TTCGAA 1 cut(s) 120
PagI TCATGA 1 cut(s) 853
PctI GAATGC 1 cut(s) 116
PfeI GAWTC 2 cut(s) 62, 695
PflFI GACNNNGTC 1 cut(s) 176
PkrI GCNGC 9 cut(s) 115, 127, 130, 277, 361, 810, 813, 823, 970
PluTI GGCGCC 1 cut(s) 208
PsiI TTATAA 1 cut(s) 636
Psp6I CCWGG 1 cut(s) 466
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 3 cut(s) 206, 869, 960
PspPI GGNCC 1 cut(s) 184
PstI CTGCAG 4 cut(s) 343, 489, 558, 816
PsuI RGATCY 1 cut(s) 661
PsyI GACNNNGTC 1 cut(s) 176
PvuII CAGCTG 1 cut(s) 811
SaqAI TTAA 3 cut(s) 374, 618, 648
SatI GCNGC 9 cut(s) 114, 126, 129, 276, 360, 809, 812, 822, 969
Sau3AI GATC 1 cut(s) 661
Sau96I GGNCC 1 cut(s) 184
ScrFI CCNGG 2 cut(s) 202, 468
SduI GDGCHC 2 cut(s) 9, 81
SfaNI GCATC 7 cut(s) 247, 510, 601, 725, 820, 848, 868
SfcI CTRYAG 4 cut(s) 339, 485, 554, 812
SfoI GGCGCC 1 cut(s) 206
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 1 cut(s) 184
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
Sse9I AATT 4 cut(s) 540, 703, 765, 940
SsiI CCGC 3 cut(s) 114, 126, 129
SspDI GGCGCC 1 cut(s) 204
SspI AATATT 1 cut(s) 794
StyD4I CCNGG 2 cut(s) 200, 466
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 1 cut(s) 382
TaqI TCGA 4 cut(s) 120, 547, 578, 769
TasI AATT 4 cut(s) 540, 703, 765, 940
TauI GCSGC 3 cut(s) 116, 128, 131
TfiI GAWTC 2 cut(s) 62, 695
Tru1I TTAA 3 cut(s) 374, 618, 648
Tru9I TTAA 3 cut(s) 374, 618, 648
TscAI CASTG 1 cut(s) 832
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 6 cut(s) 275, 359, 808, 811, 821, 968
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 3 cut(s) 521, 657, 842
TspGWI ACGGA 1 cut(s) 197
TspRI CASTG 1 cut(s) 832
Tth111I GACNNNGTC 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 184
XapI RAATTY 2 cut(s) 540, 765
Zsp2I ATGCAT 2 cut(s) 525, 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.