RchiOBHm_Chr3g0469791

Ethylene-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002662
Physical Location & Seq
Forward (+)
15695589 .. 15703807
8219 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4080 bp
ATGTCCAAGAGTCTGATAGCTCATATGGAATCTGCTAGCTCTAACGCTAACAATATGCCAGGCGTTGTCCATCAGTTGCTTCCTGTTGTTGGACCCATGCTTCTGATTGCAGTTGGATATCTTGACCCTGGAAAGTGGGCGGCAACTGTTGAAGCAGGTTCCCGTTATGGAACTGATCTGGCTGCAGTAATGCTTATATTCAATTTGGCTGCTATTTTATGTCACTATCTGTCAGCACGGATTGCTGTAGTCACTGGAAGAGATCTTGCTCAGGTTTGCAGTGAGGAATATGACAAGGCTACATGCATATTCTTAGGAGTACAAACAGAGATGTCGGTGATTGTGTTGGACCTTACCATGATCCTCGGTATCGCACATGCACTTAATCTTCTGTTTGGGTGGGACTTGTTCACATGTGTGTTTTTGACTGCTGCTAATGCTGTTTTATACCCTCTTTTTTCCACCCTCCTGGAGACTTGCAAGGCGAAATTCCTTTGCGTATGCATATACATTGCAGGATTTCTACTGCTTTCCTTTGTTCTTGGAGTATTTATCAGTCAACCACAAGTGCCACTTTCCATGACTGGGATGTTAACAAAACTGAGTGGGGAAAGTGCTTTTTCACTGATGAGTCTTCTTGGAGCAAGTATAATGCCCCACAGTTTTTATCTTCATTCTTCTATTGTGCAGCAGCATCAGCAACAACCAACTGTTTCTAAGGATGCCTTGTGTCAGAACCATTTTGTTGCCATCTTCTGCACCTTTAGTGGTATTTATCTGGTGAATTATGCCCTCATGACCTTAGCAGCAAATGTATTCTACACTTCACGTGGTTTGCTCACATTTCAGGATGCAATGTCCCTAATGGAACAGGTCTTTTGGGGTCCAATAGTACCTGTTGCCTTCTTGCTAGTTCTCTTTTTATCAAATCAAATCACAACATTAAGCTGGAGTCTGGGGGGACAAGTAGTCTTGAATGATTTTTTAAAACTAGACCTTCCTGGTTGGCTTCACTGTGCTACAATCAGAATCATTGCCATTGTTCCTGCTCTGTATTTTGTTTGGAGCTCAGGAGCTGAGGGGATGTACCAACTGCTTATATCTACACAGGTATTGGCAGCCTTGCTACTGCCGTCTTCTGTGATCCCTCTTTTTCGTGTTGCTGCTTCAAGACAAATAATGGGAGCCCATAAGATCTCTCAGTTCGTTGAATTCTTGGCCCTCATTACACTTATTGGGATGCTTGGATTAAAACTTGTCTTTGTAGTGGAAATGATTTTTGGGAATAGTGATTGGGTGGATAATTTGAGATGGGATGCTGGGAGTAGTATGTCTGCACTTCTCATCACTGCTTCTGCATCATTTTGTTTGATGATTTGGCTGGCAGCTACACCGTTAAAATCTGCAAGTGCTCGATTAGAGAACCAGGTGTGGAACTGGGATATGCCTAAGGGTGTATCTGAGCCATTTAGAAATAGGGAGGAGATTGATATAGCTGAACCTAATTATCATAGAGATGCAAGTGTTCAGAAGCATGAACCATCACCATCTTCTGGGAAGGCTTTGGATAGAGACTCGGATACAGCGGTTGCAAATTTTGATTTTGTTCTGCCCGAAACTCTCTTGGAGCCTGATCAGGAGCTTCAATCAACTACCTCAGAGGAAAATAGTTCTCTTAATACCTTTCCTCGCTCTGCCAAATGCAATAAGGAGGAAACCACATCTGTGGTGGAGTCAATTCGCCTCCCAACTGTGGCTAGTGAGGTTTCTGATGTTACATCACTGGGCACCAGTACTGTGAAAGTTGGATCAACAGAGCCAGTTGAGAAGATTCTTGGAGTTGAAGGAGACTTACCAACTGAAAAAGATGATGATGAGGGAGATACCTGGGAACCTGAAGATTCCTTGAAAGAGGTTTCTGGGGGCACCACTTCATTGACATCTGAAGGTCCAGGATCATTCAGGAGTCTCAGTGGAAAAGGTGATGAAGGGGGGAGTGGTGCTGGAAGCCTTTCAAGATTAGCAGGGTTGGGGCGTGCTGCTAGACGTCAACTGGCTGCAGTACTCGATGAGTTTTGGGGACAACTGTATGATTTCCATGGTAATGTAATTCAAGAAGCAAAGGCTAGGAAACTGGATCTGTTGTTGGGGTCAGATTCAAAGGCTTCTTCCTCCGCTTCCTCCGTGCTGAAAGATGATACCACTGCAAAGGAAGTTTCTGGATACTTTCCATCAGTAGGAGGCAGAGGATCTGATCCTTTAATCAACTCAAGTTTATATGACTCCATAAATCAGCCAAGGCTGCAAAACAGTTTAGAGTCATCATATGGTGCTCAAAGGGGATCTTCCTCATTATGGTCTGGCCACATACAGTTGCTGGATGCGTATGGACAAAATTCAACCTGCAGTATTATTGACTCGGGTGAGAGGCGCTATTCAAGTGTGCGCAGTATACCATCTTCAGACTTGGGTGAGAGGCGCTATTCAAGTGTGCGCAGTATACCATCTTCAGACTTGGGTGAGAGGCGCTATTCAAGTGTGCGCAGTATACCAACTTCTGACTCGGGTGAGAGGCGCTATTCAAGTGTCCGCAGCATACCATCTGCTGAGAGCTGGGATTACCAGCCAGCCACAGTACACGGTTATCAGATTTCATCGTATCTTAATAGTAATGACAGAAGTTCTGGTAACTTGAATGGTCAAATGGAATCACCAGCCCTAAATTCTGCTTCTTCATTGGGTGCTGGAAACTACAGAGAGTCACTTGCATTTACCATGGGGCAGAAGTTGCAAAATGGGTTAGGCTCTGGACAGGCCTCCAGTTTTCAGAACCTTACGGTATCTCGACATAGTCCGTTGCAATCTGAAAGACCATATTATGATGTACACTCTTCTGGAATTTCTGAGAATGTGGTAAATTCAGCCAATGCAAAGAAATACCACAGTTTACCTGACATTCACCGCGATCTCTACATGTCTAATAAAAGTGCTCAACGGGATGCTCCCCCTGGGTTTGGGAAAACTAATTATGAATCATCCTTGTATCCAAAATCTGTTGCACGGACAGGAGGTCCTTTGGCATTTGATGAACTCTCTCCATCAAAAGTCTATAGAGATGTTCTATCATCCCAACAGAATTCTAATTTTGGCACTGGATCTCTCTGGTCTAGACAGCCTTTTGAGCAATTTGGTGTAGCTGATAATAATCGTTCTATTGGGACTGCAGTTGGTAGTAGAGCAGGTTCTGCAGGTCAGGAAGCTATGTCAGTTGCAGATTCAGAGGCGAAGCTTCTTCAGTCTTTTAGACACTGCATTGTGAAGCTTTTGAAGTTGGAAGGGTCTGACTGGTTGTTTAGACAGAATGATGGAGTGGATGAGGATCTAATAGATCGTGTGGCTGCCAGGGAGAAAATTATTTATGAAGCTGAAACTAGAGAGATTAATCGAACAGTTCACATGGGTGAATCTCAGTACACTTCTGACAGGAAGTCAACTTCTGCAATAAAAATGAGTGATGCAAATCTCACCCATCTCATGGTTTCCTCAGTTCCTAATTGTGGGGAAGGTTGTATCTGGAGATCTGATCTAATAATAAGCTTTGGGGTCTGGTGTATCCATCGGATTCTTGATTTGTCCCTTATGGAAAGCCGGCCAGAGCTCTGGGGGAAATATACTTACGTTCTCAATCGACTTCAGGGGATTATTGATTCTGCGTTTTCGAAGCCTCGTTCTCCAATGTCTCCATGCTTCTGCCTTCAAATTGCAGCAGCACAGCAGCAGAAGTCCAGTCCAACATTTTCGAATGGAATGTTACCCCCTGCTGCGAAACCAGCCAGGGGAAAATGCACTACAGCTGCAACACTCGCGGACATAATCAAGGATGTGGAGACTGCAATCTCTTGTCGAAAGGGCCGAACGGGGACGGCAGCCGGTGATGTGGCTTTCCCGAAGGGAAAAGAAAATCTGGCATCTGTCCTCAAACGCTACAAGCGGCGATTATCCAACAAACCTGTTTGCACTCAGGAGGGGCCTTCCCGTTCACGGAAGGGTGCTCCAACATCTGCTCCTTATGGGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001736 GO:0001738 GO:0002009 GO:0002237 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007164 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009787 GO:0009789 GO:0009814 GO:0009861 GO:0009871 GO:0009873 GO:0009888 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010053 GO:0010054 GO:0010087 GO:0010104 GO:0010119 GO:0010150 GO:0010182 GO:0010646 GO:0010647 GO:0010817 GO:0016043 GO:0021700 GO:0022622 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0031347 GO:0031348 GO:0032501 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0035556 GO:0042221 GO:0042545 GO:0042742 GO:0043207 GO:0045087 GO:0045229 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048585 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060429 GO:0060918 GO:0065007 GO:0065008 GO:0070297 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0080134 GO:0090558 GO:0090627 GO:0090693 GO:0097159 GO:0098542 GO:0099402 GO:1901363 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1902531 GO:1905392 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
Nramp

Protein Analysis

1359

Amino Acids

147.56

Weight (kDa)

6.04

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nramp PF01566 46 - 400 1.1e-83 Natural resistance-associated macrophage protein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2050
Acc16I TGCGCA 3 cut(s) 2447, 2495, 2543
Acc36I ACCTGC 4 cut(s) 146, 2411, 3230, 3239
AccB1I GGYRCC 2 cut(s) 1787, 1925
AccB7I CCANNNNNTGG 2 cut(s) 1553, 3535
AccI GTMKAC 3 cut(s) 2452, 2500, 2548
AccII CGCG 2 cut(s) 2964, 3866
AciI CCGC 7 cut(s) 140, 1586, 2175, 2590, 2962, 3866, 3991
AcoI YGGCCR 2 cut(s) 2362, 3650
AcsI RAATTY 8 cut(s) 488, 1211, 1594, 2395, 2722, 2898, 2917, 3136
AcuI CTGAAG 6 cut(s) 1917, 1965, 2445, 2493, 3278, 3677
AcvI CACGTG 1 cut(s) 830
AcyI GRCGYC 1 cut(s) 2047
AfaI GTAC 8 cut(s) 321, 894, 1088, 1795, 2064, 2637, 2886, 3473
AflIII ACRYGT 2 cut(s) 413, 2973
AhdI GACNNNNNGTC 3 cut(s) 968, 3069, 3487
AjuI GAANNNNNNNTTGG 2 cut(s) 1263, 1295
AleI CACNNNNGTG 3 cut(s) 416, 1724, 3459
Alw21I GWGCWC 6 cut(s) 1070, 1414, 2335, 2992, 3660, 4054
Alw26I GTCTC 6 cut(s) 467, 1566, 1842, 1973, 3744, 3881
AlwNI CAGNNNCTG 3 cut(s) 1076, 2377, 3245
Ama87I CYCGRG 2 cut(s) 2419, 2563
AoxI GGCC 6 cut(s) 1218, 2362, 2814, 3650, 3910, 4028
ApoI RAATTY 8 cut(s) 488, 1211, 1594, 2395, 2722, 2898, 2917, 3136
AseI ATTAAT 1 cut(s) 3441
Asp700I GAANNNNTTC 1 cut(s) 2011
AspLEI GCGC 7 cut(s) 2433, 2448, 2481, 2496, 2529, 2544, 2577
AspS9I GGNCC 8 cut(s) 92, 349, 884, 1219, 1949, 3071, 3910, 4028
AsuII TTCGAA 2 cut(s) 3719, 3800
AsuNHI GCTAGC 1 cut(s) 35
AvaI CYCGRG 2 cut(s) 2419, 2563
AvaII GGWCC 5 cut(s) 92, 349, 884, 1949, 3071
AxyI CCTNAGG 1 cut(s) 1449
BaeGI GKGCMC 2 cut(s) 1790, 1928
BaeI ACNNNNGTAYC 2 cut(s) 1572, 1605
BalI TGGCCA 1 cut(s) 2364
BanI GGYRCC 2 cut(s) 1787, 1925
BanII GRGCYC 3 cut(s) 1070, 1189, 3660
BarI GAAGNNNNNNTAC 8 cut(s) 1836, 1868, 2443, 2475, 2491, 2523, 2539, 2571
BbrPI CACGTG 1 cut(s) 830
BbsI GAAGAC 2 cut(s) 626, 1128
Bbv12I GWGCWC 6 cut(s) 1070, 1414, 2335, 2992, 3660, 4054
BbvCI CCTCAGC 1 cut(s) 1077
BceAI ACGGC 2 cut(s) 1117, 3939
BciVI GTATCC 4 cut(s) 1573, 2216, 3054, 3623
BclI TGATCA 1 cut(s) 1633
BcoDI GTCTC 6 cut(s) 467, 1566, 1842, 1973, 3744, 3881
BfaI CTAG 8 cut(s) 36, 911, 992, 1758, 2043, 2127, 3168, 3432
BfmI CTRYAG 9 cut(s) 183, 246, 2058, 2404, 2752, 3109, 3222, 3246, 3849
BfoI RGCGCY 4 cut(s) 2434, 2482, 2530, 2578
BfuAI ACCTGC 4 cut(s) 146, 2411, 3230, 3239
BfuI GTATCC 4 cut(s) 1573, 2216, 3054, 3623
BglII AGATCT 3 cut(s) 262, 1194, 3578
BmcAI AGTACT 2 cut(s) 1795, 2064
Bme18I GGWCC 5 cut(s) 92, 349, 884, 1949, 3071
BmeRI GACNNNNNGTC 3 cut(s) 968, 3069, 3487
BmeT110I CYCGRG 2 cut(s) 2419, 2563
BmgT120I GGNCC 8 cut(s) 92, 349, 884, 1219, 1949, 3071, 3910, 4028
BmiI GGNNCC 9 cut(s) 94, 160, 885, 1186, 1629, 1789, 1893, 1927, 4029
BmrI ACTGGG 3 cut(s) 594, 1447, 1793
BmtI GCTAGC 1 cut(s) 39
BmuI ACTGGG 3 cut(s) 594, 1447, 1793
BpiI GAAGAC 2 cut(s) 626, 1128
BpmI CTGGAG 4 cut(s) 491, 970, 2803, 3595
Bpu10I CCTNAGC 4 cut(s) 270, 802, 1069, 1077
Bpu14I TTCGAA 2 cut(s) 3719, 3800
BpuEI CTTGAG 1 cut(s) 2254
BsaAI YACGTR 1 cut(s) 830
BsaBI GATNNNNATC 3 cut(s) 3204, 3378, 3519
BsaHI GRCGYC 1 cut(s) 2047
BsaXI ACNNNNNCTCC 4 cut(s) 1957, 1987, 4048, 4078
Bse118I RCCGGY 2 cut(s) 3648, 3929
Bse21I CCTNAGG 1 cut(s) 1449
Bse3DI GCAATG 3 cut(s) 510, 861, 1032
Bse8I GATNNNNATC 3 cut(s) 3204, 3378, 3519
BseJI GATNNNNATC 3 cut(s) 3204, 3378, 3519
BseMI GCAATG 3 cut(s) 510, 861, 1032
BseRI GAGGAG 1 cut(s) 1496
BseSI GKGCMC 2 cut(s) 1790, 1928
BseYI CCCAGC 2 cut(s) 1319, 2613
BsgI GTGCAG 3 cut(s) 707, 742, 1320
Bsh1236I CGCG 2 cut(s) 2964, 3866
BshFI GGCC 6 cut(s) 1220, 2364, 2816, 3652, 3912, 4030
BshNI GGYRCC 2 cut(s) 1787, 1925
BsiHKAI GWGCWC 6 cut(s) 1070, 1414, 2335, 2992, 3660, 4054
BsiHKCI CYCGRG 2 cut(s) 2419, 2563
BsiSI CCGG 2 cut(s) 3649, 3930
BslFI GGGAC 7 cut(s) 416, 844, 975, 2094, 3232, 3619, 3934
BsmAI GTCTC 6 cut(s) 467, 1566, 1842, 1973, 3744, 3881
BsmFI GGGAC 7 cut(s) 416, 844, 975, 2094, 3232, 3619, 3934
BsnI GGCC 6 cut(s) 1220, 2364, 2816, 3652, 3912, 4030
BsoBI CYCGRG 2 cut(s) 2419, 2563
Bsp119I TTCGAA 2 cut(s) 3719, 3800
Bsp1286I GDGCHC 9 cut(s) 1070, 1189, 1414, 1790, 1928, 2335, 2992, 3660, 4054
Bsp1407I TGTACA 1 cut(s) 2884
Bsp19I CCATGG 2 cut(s) 2098, 2775
BspACI CCGC 7 cut(s) 140, 1586, 2175, 2590, 2962, 3866, 3991
BspANI GGCC 6 cut(s) 1220, 2364, 2816, 3652, 3912, 4030
BspFNI CGCG 2 cut(s) 2964, 3866
BspHI TCATGA 1 cut(s) 795
BspLI GGNNCC 9 cut(s) 94, 160, 885, 1186, 1629, 1789, 1893, 1927, 4029
BspMAI CTGCAG 5 cut(s) 187, 2062, 2408, 3226, 3250
BspMI ACCTGC 4 cut(s) 146, 2411, 3230, 3239
BspOI GCTAGC 1 cut(s) 39
BspT104I TTCGAA 2 cut(s) 3719, 3800
BspT107I GGYRCC 2 cut(s) 1787, 1925
BsrDI GCAATG 3 cut(s) 510, 861, 1032
BsrFI RCCGGY 2 cut(s) 3648, 3929
BsrGI TGTACA 1 cut(s) 2884
BssAI RCCGGY 2 cut(s) 3648, 3929
BssNAI GTATAC 3 cut(s) 2453, 2501, 2549
BssNI GRCGYC 1 cut(s) 2047
BssT1I CCWWGG 3 cut(s) 2098, 2297, 2775
Bst1107I GTATAC 3 cut(s) 2453, 2501, 2549
Bst6I CTCTTC 2 cut(s) 253, 2896
BstACI GRCGYC 1 cut(s) 2047
BstAPI GCANNNNNTGC 3 cut(s) 242, 2788, 3245
BstAUI TGTACA 1 cut(s) 2884
BstBAI YACGTR 1 cut(s) 830
BstBI TTCGAA 2 cut(s) 3719, 3800
BstC8I GCNNGC 5 cut(s) 37, 1383, 2037, 2628, 3650
BstDSI CCRYGG 2 cut(s) 2098, 2775
BstFNI CGCG 2 cut(s) 2964, 3866
BstH2I RGCGCY 4 cut(s) 2434, 2482, 2530, 2578
BstHHI GCGC 7 cut(s) 2433, 2448, 2481, 2496, 2529, 2544, 2577
BstMAI GTCTC 6 cut(s) 467, 1566, 1842, 1973, 3744, 3881
BstMWI GCNNNNNNNGC 9 cut(s) 242, 437, 697, 2302, 2788, 3181, 3245, 3830, 3863
BstNSI RCATGY 4 cut(s) 306, 380, 417, 2977
BstSFI CTRYAG 9 cut(s) 183, 246, 2058, 2404, 2752, 3109, 3222, 3246, 3849
BstSLI GKGCMC 2 cut(s) 1790, 1928
BstUI CGCG 2 cut(s) 2964, 3866
BstV2I GAAGAC 2 cut(s) 626, 1128
BstX2I RGATCY 8 cut(s) 262, 1194, 2137, 2249, 2342, 3155, 3379, 3578
BstXI CCANNNNNNTGG 3 cut(s) 469, 1726, 3660
BstYI RGATCY 8 cut(s) 262, 1194, 2137, 2249, 2342, 3155, 3379, 3578
BstZ17I GTATAC 3 cut(s) 2453, 2501, 2549
Bsu36I CCTNAGG 1 cut(s) 1449
BsuI GTATCC 4 cut(s) 1573, 2216, 3054, 3623
BsuRI GGCC 6 cut(s) 1220, 2364, 2816, 3652, 3912, 4030
BtgI CCRYGG 2 cut(s) 2098, 2775
BtsI GCAGTG 4 cut(s) 286, 1347, 2202, 3307
BveI ACCTGC 4 cut(s) 146, 2411, 3230, 3239
Cac8I GCNNGC 5 cut(s) 37, 1383, 2037, 2628, 3650
CaiI CAGNNNCTG 3 cut(s) 1076, 2377, 3245
CciI TCATGA 1 cut(s) 795
CfoI GCGC 7 cut(s) 2433, 2448, 2481, 2496, 2529, 2544, 2577
Cfr10I RCCGGY 2 cut(s) 3648, 3929
Cfr13I GGNCC 8 cut(s) 92, 349, 884, 1219, 1949, 3071, 3910, 4028
CsiI ACCWGGT 1 cut(s) 1425
Csp6I GTAC 8 cut(s) 320, 893, 1087, 1794, 2063, 2636, 2885, 3472
CviQI GTAC 8 cut(s) 320, 893, 1087, 1794, 2063, 2636, 2885, 3472
DraI TTTAAA 1 cut(s) 987
DriI GACNNNNNGTC 3 cut(s) 968, 3069, 3487
EaeI YGGCCR 2 cut(s) 2362, 3650
Eam1104I CTCTTC 2 cut(s) 253, 2896
Eam1105I GACNNNNNGTC 3 cut(s) 968, 3069, 3487
EarI CTCTTC 2 cut(s) 253, 2896
Ecl136II GAGCTC 2 cut(s) 1068, 3658
Eco130I CCWWGG 3 cut(s) 2098, 2297, 2775
Eco147I AGGCCT 1 cut(s) 2816
Eco24I GRGCYC 3 cut(s) 1070, 1189, 3660
Eco32I GATATC 1 cut(s) 119
Eco47I GGWCC 5 cut(s) 92, 349, 884, 1949, 3071
Eco53kI GAGCTC 2 cut(s) 1068, 3658
Eco57I CTGAAG 6 cut(s) 1917, 1965, 2445, 2493, 3278, 3677
Eco72I CACGTG 1 cut(s) 830
Eco81I CCTNAGG 1 cut(s) 1449
Eco88I CYCGRG 2 cut(s) 2419, 2563
EcoICRI GAGCTC 2 cut(s) 1068, 3658
EcoO109I RGGNCCY 2 cut(s) 3071, 4028
EcoRI GAATTC 2 cut(s) 1211, 3136
EcoRV GATATC 1 cut(s) 119
EcoT14I CCWWGG 3 cut(s) 2098, 2297, 2775
EcoT22I ATGCAT 2 cut(s) 308, 506
EcoT38I GRGCYC 3 cut(s) 1070, 1189, 3660
ErhI CCWWGG 3 cut(s) 2098, 2297, 2775
FalI AAGNNNNNCTT 6 cut(s) 558, 590, 710, 742, 2329, 2361
FaqI GGGAC 7 cut(s) 416, 844, 975, 2094, 3232, 3619, 3934
FauNDI CATATG 2 cut(s) 24, 2326
FbaI TGATCA 1 cut(s) 1633
FblI GTMKAC 3 cut(s) 2452, 2500, 2548
FriOI GRGCYC 3 cut(s) 1070, 1189, 3660
FspBI CTAG 8 cut(s) 36, 911, 992, 1758, 2043, 2127, 3168, 3432
FspI TGCGCA 3 cut(s) 2447, 2495, 2543
GlaI GCGC 7 cut(s) 2432, 2447, 2480, 2495, 2528, 2543, 2576
GsaI CCCAGC 2 cut(s) 1323, 2617
GsuI CTGGAG 4 cut(s) 491, 970, 2803, 3595
HaeII RGCGCY 4 cut(s) 2434, 2482, 2530, 2578
HaeIII GGCC 6 cut(s) 1220, 2364, 2816, 3652, 3912, 4030
HapII CCGG 2 cut(s) 3649, 3930
HhaI GCGC 7 cut(s) 2433, 2448, 2481, 2496, 2529, 2544, 2577
Hin1I GRCGYC 1 cut(s) 2047
Hin6I GCGC 7 cut(s) 2431, 2446, 2479, 2494, 2527, 2542, 2575
HinP1I GCGC 7 cut(s) 2431, 2446, 2479, 2494, 2527, 2542, 2575
HincII GTYRAC 4 cut(s) 560, 594, 2051, 3492
HindII GTYRAC 4 cut(s) 560, 594, 2051, 3492
HindIII AAGCTT 3 cut(s) 3287, 3320, 3595
HpaI GTTAAC 1 cut(s) 594
HpaII CCGG 2 cut(s) 3649, 3930
HpyCH4IV ACGT 3 cut(s) 829, 2047, 3678
HpyF10VI GCNNNNNNNGC 9 cut(s) 242, 437, 697, 2302, 2788, 3181, 3245, 3830, 3863
HpySE526I ACGT 3 cut(s) 829, 2047, 3678
Hsp92I GRCGYC 1 cut(s) 2047
HspAI GCGC 7 cut(s) 2431, 2446, 2479, 2494, 2527, 2542, 2575
KroI GCCGGC 1 cut(s) 3648
KroNI GCCGGC 1 cut(s) 3650
Ksp22I TGATCA 1 cut(s) 1633
KspAI GTTAAC 1 cut(s) 594
LmnI GCTCC 9 cut(s) 641, 1065, 1073, 1184, 1627, 1639, 3007, 4057, 4069
MabI ACCWGGT 1 cut(s) 1425
MaeI CTAG 8 cut(s) 36, 911, 992, 1758, 2043, 2127, 3168, 3432
MaeII ACGT 3 cut(s) 829, 2047, 3678
MaeIII GTNAC 6 cut(s) 221, 250, 1774, 2687, 2760, 3810
MflI RGATCY 8 cut(s) 262, 1194, 2137, 2249, 2342, 3155, 3379, 3578
MhlI GDGCHC 9 cut(s) 1070, 1189, 1414, 1790, 1928, 2335, 2992, 3660, 4054
MlsI TGGCCA 1 cut(s) 2364
MluNI TGGCCA 1 cut(s) 2364
MmeI TCCRAC 8 cut(s) 70, 94, 327, 1786, 3312, 3815, 4026, 4079
Mox20I TGGCCA 1 cut(s) 2364
Mph1103I ATGCAT 2 cut(s) 308, 506
MroNI GCCGGC 1 cut(s) 3648
MroXI GAANNNNTTC 1 cut(s) 2011
MscI TGGCCA 1 cut(s) 2364
MslI CAYNNNNRTG 5 cut(s) 416, 1515, 1724, 2103, 3459
Msp20I TGGCCA 1 cut(s) 2364
MspA1I CMGCKG 2 cut(s) 1586, 3854
MspI CCGG 2 cut(s) 3649, 3930
MvnI CGCG 2 cut(s) 2964, 3866
MwoI GCNNNNNNNGC 9 cut(s) 242, 437, 697, 2302, 2788, 3181, 3245, 3830, 3863
NaeI GCCGGC 1 cut(s) 3650
NcoI CCATGG 2 cut(s) 2098, 2775
NdeI CATATG 2 cut(s) 24, 2326
NgoMIV GCCGGC 1 cut(s) 3648
NheI GCTAGC 1 cut(s) 35
NlaIV GGNNCC 9 cut(s) 94, 160, 885, 1186, 1629, 1789, 1893, 1927, 4029
NmuCI GTSAC 3 cut(s) 221, 250, 2760
NsbI TGCGCA 3 cut(s) 2447, 2495, 2543
NsiI ATGCAT 2 cut(s) 308, 506
NspI RCATGY 4 cut(s) 306, 380, 417, 2977
NspV TTCGAA 2 cut(s) 3719, 3800
OliI CACNNNNGTG 3 cut(s) 416, 1724, 3459
PagI TCATGA 1 cut(s) 795
PasI CCCWGGG 1 cut(s) 3008
PceI AGGCCT 1 cut(s) 2816
PciI ACATGT 2 cut(s) 413, 2973
PcsI WCGNNNNNNNCGW 1 cut(s) 3910
PdiI GCCGGC 1 cut(s) 3650
PdmI GAANNNNTTC 1 cut(s) 2011
PflFI GACNNNGTC 1 cut(s) 2850
PflMI CCANNNNNTGG 2 cut(s) 1553, 3535
PfoI TCCNGGA 2 cut(s) 468, 1951
PmaCI CACGTG 1 cut(s) 830
PmlI CACGTG 1 cut(s) 830
Ppu21I YACGTR 1 cut(s) 830
PpuMI RGGWCCY 1 cut(s) 3071
PscI ACATGT 2 cut(s) 413, 2973
PshBI ATTAAT 1 cut(s) 3441
Psp124BI GAGCTC 2 cut(s) 1070, 3660
Psp5II RGGWCCY 1 cut(s) 3071
PspCI CACGTG 1 cut(s) 830
PspFI CCCAGC 2 cut(s) 1319, 2613
PspN4I GGNNCC 9 cut(s) 94, 160, 885, 1186, 1629, 1789, 1893, 1927, 4029
PspPI GGNCC 8 cut(s) 92, 349, 884, 1219, 1949, 3071, 3910, 4028
PspPPI RGGWCCY 1 cut(s) 3071
PsrI GAACNNNNNNTAC 2 cut(s) 3226, 3258
PstI CTGCAG 5 cut(s) 187, 2062, 2408, 3226, 3250
PstNI CAGNNNCTG 3 cut(s) 1076, 2377, 3245
PsuI RGATCY 8 cut(s) 262, 1194, 2137, 2249, 2342, 3155, 3379, 3578
PsyI GACNNNGTC 1 cut(s) 2850
PvuII CAGCTG 1 cut(s) 3854
RsaI GTAC 8 cut(s) 321, 894, 1088, 1795, 2064, 2637, 2886, 3473
RsaNI GTAC 8 cut(s) 320, 893, 1087, 1794, 2063, 2636, 2885, 3472
RseI CAYNNNNRTG 5 cut(s) 416, 1515, 1724, 2103, 3459
SacI GAGCTC 2 cut(s) 1070, 3660
Sau96I GGNCC 8 cut(s) 92, 349, 884, 1219, 1949, 3071, 3910, 4028
ScaI AGTACT 2 cut(s) 1795, 2064
SduI GDGCHC 9 cut(s) 1070, 1189, 1414, 1790, 1928, 2335, 2992, 3660, 4054
SexAI ACCWGGT 1 cut(s) 1425
SfcI CTRYAG 9 cut(s) 183, 246, 2058, 2404, 2752, 3109, 3222, 3246, 3849
SfuI TTCGAA 2 cut(s) 3719, 3800
SinI GGWCC 5 cut(s) 92, 349, 884, 1949, 3071
SmiMI CAYNNNNRTG 5 cut(s) 416, 1515, 1724, 2103, 3459
SmlI CTYRAG 1 cut(s) 2269
SmoI CTYRAG 1 cut(s) 2269
SseBI AGGCCT 1 cut(s) 2816
SsiI CCGC 7 cut(s) 140, 1586, 2175, 2590, 2962, 3866, 3991
SspMI CTAG 8 cut(s) 36, 911, 992, 1758, 2043, 2127, 3168, 3432
SstI GAGCTC 2 cut(s) 1070, 3660
StuI AGGCCT 1 cut(s) 2816
StyI CCWWGG 3 cut(s) 2098, 2297, 2775
TaiI ACGT 3 cut(s) 832, 2050, 3681
TaqI TCGA 8 cut(s) 1414, 2067, 2845, 3445, 3688, 3719, 3800, 3904
TatI WGTACW 6 cut(s) 319, 1793, 2062, 2635, 2884, 3471
TauI GCSGC 2 cut(s) 143, 3994
TseFI GTSAC 3 cut(s) 221, 250, 2760
Tsp45I GTSAC 3 cut(s) 221, 250, 2760
TspDTI ATGAA 9 cut(s) 662, 1551, 1923, 2001, 2643, 2724, 3045, 3102, 3435
TspGWI ACGGA 5 cut(s) 253, 2173, 2844, 3076, 4057
Tth111I GACNNNGTC 1 cut(s) 2850
Van91I CCANNNNNTGG 2 cut(s) 1553, 3535
VpaK11BI GGWCC 5 cut(s) 92, 349, 884, 1949, 3071
VspI ATTAAT 1 cut(s) 3441
XapI RAATTY 8 cut(s) 488, 1211, 1594, 2395, 2722, 2898, 2917, 3136
XbaI TCTAGA 1 cut(s) 3167
XceI RCATGY 4 cut(s) 306, 380, 417, 2977
XcmI CCANNNNNNNNNTGG 1 cut(s) 1726
XmiI GTMKAC 3 cut(s) 2452, 2500, 2548
XmnI GAANNNNTTC 1 cut(s) 2011
XspI CTAG 8 cut(s) 36, 911, 992, 1758, 2043, 2127, 3168, 3432
ZraI GACGTC 1 cut(s) 2048
ZrmI AGTACT 2 cut(s) 1795, 2064
Zsp2I ATGCAT 2 cut(s) 308, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.