RchiOBHm_Chr2g0088451

Signal recognition particle 54 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0016992
Physical Location & Seq
Forward (+)
2992797 .. 2995848
3052 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 627 bp
ATGACAATGGGGCTAGATTTGCAGAAGCTGTACAGAAGTTGGGAAGATAATCTGGGCGTTGGTTTTATTGCATTGAAGGTAAAAGTGCCTGTATATACAGCAGGGACTGACACCAAACCTTCACAAATAGCTAGGCAAGGTTTAGAGGAGGCCAAAAAGAAGAACATAGATCTAGTAATAATGGATACTGCTGGAAGGCTTCAGATAGACAAAGTACTGATGGATGAGTTGAAAGAAGTAAAGTGGGAATTGAACCCCACAGGAAACTTTACTCATTGTGGATGCAATGACTGGCCAGGAAGCTGCAGAAAATCCCTGGTCACAACATTCAATGTAGAGATTGGGATTACTGGCGCTATTTTAACAAAGCTGGATGGAGATTCTAAAGGTGGAGCAGCTTTGAGTGTTAAAGAGGTTTACTTGTGTAAAGCAAGGACAGCAGCTGCAATCAAAGTTCCAAAGTTTCAATTTGAGGAAGGAATTATAGAGACCACATACCTTCAAGAAGAAGTCAATGAACTCCATGATGGGAAGAATTCGTTAGCGATAAGTATCTTTAGACTTTTACAATGTGGATCTTCATCAATTGTATGGTTTAACTCTGTACCTAGTGAAACTTCTTTGTAG

Protein Analysis

208

Amino Acids

22.9

Weight (kDa)

5.79

Isoelectric Point (pI)

29.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP54 PF00448 23 - 138 7.8e-27 SRP54-type protein, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 583
AcoI YGGCCR 1 cut(s) 293
AcsI RAATTY 1 cut(s) 535
AcuI CTGAAG 1 cut(s) 185
AfaI GTAC 3 cut(s) 32, 216, 606
AgsI TTSAA 6 cut(s) 76, 232, 253, 331, 467, 503
AjnI CCWGG 2 cut(s) 295, 315
AluBI AGCT 6 cut(s) 28, 131, 303, 370, 398, 443
AluI AGCT 6 cut(s) 28, 131, 303, 370, 398, 443
Alw26I GTCTC 1 cut(s) 482
AlwI GGATC 1 cut(s) 583
AlwNI CAGNNNCTG 3 cut(s) 28, 107, 443
AoxI GGCC 2 cut(s) 150, 293
ApeKI GCWGC 4 cut(s) 303, 395, 440, 443
ApoI RAATTY 1 cut(s) 535
AspLEI GCGC 1 cut(s) 356
BalI TGGCCA 1 cut(s) 295
BbvI GCAGC 4 cut(s) 290, 407, 430, 452
BccI CCATC 3 cut(s) 214, 368, 521
BciT130I CCWGG 2 cut(s) 297, 317
BciVI GTATCC 1 cut(s) 178
BcoDI GTCTC 1 cut(s) 482
BfaI CTAG 4 cut(s) 14, 132, 173, 609
BfmI CTRYAG 1 cut(s) 304
BfoI RGCGCY 1 cut(s) 357
BfuI GTATCC 1 cut(s) 178
BglII AGATCT 1 cut(s) 169
BisI GCNGC 4 cut(s) 304, 396, 441, 444
BlsI GCNGC 4 cut(s) 305, 397, 442, 445
BmcAI AGTACT 1 cut(s) 216
Bme1390I CCNGG 2 cut(s) 297, 317
BmrFI CCNGG 2 cut(s) 297, 317
BmsI GCATC 1 cut(s) 272
BsaBI GATNNNNATC 2 cut(s) 551, 580
BsaI GGTCTC 1 cut(s) 482
BsaJI CCNNGG 1 cut(s) 315
Bse1I ACTGG 2 cut(s) 296, 355
Bse3DI GCAATG 1 cut(s) 292
Bse8I GATNNNNATC 2 cut(s) 551, 580
BseBI CCWGG 2 cut(s) 297, 317
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 3 cut(s) 229, 287, 379
BseJI GATNNNNATC 2 cut(s) 551, 580
BseMI GCAATG 1 cut(s) 292
BseNI ACTGG 2 cut(s) 296, 355
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 4 cut(s) 290, 407, 430, 452
BshFI GGCC 2 cut(s) 152, 295
BslFI GGGAC 1 cut(s) 118
BsmAI GTCTC 1 cut(s) 482
BsmFI GGGAC 1 cut(s) 118
BsnI GGCC 2 cut(s) 152, 295
Bso31I GGTCTC 1 cut(s) 482
Bsp1407I TGTACA 1 cut(s) 30
Bsp143I GATC 2 cut(s) 169, 575
BspANI GGCC 2 cut(s) 152, 295
BspMAI CTGCAG 1 cut(s) 308
BspPI GGATC 1 cut(s) 583
BspTNI GGTCTC 1 cut(s) 482
BsrDI GCAATG 1 cut(s) 292
BsrGI TGTACA 1 cut(s) 30
BsrI ACTGG 2 cut(s) 296, 355
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 2 cut(s) 169, 575
Bst2UI CCWGG 2 cut(s) 297, 317
BstAUI TGTACA 1 cut(s) 30
BstF5I GGATG 3 cut(s) 229, 287, 379
BstH2I RGCGCY 1 cut(s) 357
BstHHI GCGC 1 cut(s) 356
BstKTI GATC 2 cut(s) 172, 578
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 2 cut(s) 169, 575
BstMWI GCNNNNNNNGC 2 cut(s) 19, 437
BstNI CCWGG 2 cut(s) 297, 317
BstSCI CCNGG 2 cut(s) 295, 315
BstSFI CTRYAG 1 cut(s) 304
BstV1I GCAGC 4 cut(s) 290, 407, 430, 452
BstX2I RGATCY 2 cut(s) 169, 575
BstYI RGATCY 2 cut(s) 169, 575
BsuI GTATCC 1 cut(s) 178
BsuRI GGCC 2 cut(s) 152, 295
BtsCI GGATG 3 cut(s) 229, 287, 379
CaiI CAGNNNCTG 3 cut(s) 28, 107, 443
CfoI GCGC 1 cut(s) 356
Csp6I GTAC 3 cut(s) 31, 215, 605
CviAII CATG 1 cut(s) 524
CviQI GTAC 3 cut(s) 31, 215, 605
DpnI GATC 2 cut(s) 171, 577
DpnII GATC 2 cut(s) 169, 575
EaeI YGGCCR 1 cut(s) 293
Eco31I GGTCTC 1 cut(s) 482
Eco57I CTGAAG 1 cut(s) 185
EcoRI GAATTC 1 cut(s) 535
EcoRII CCWGG 2 cut(s) 295, 315
FaeI CATG 1 cut(s) 527
FaiI YATR 7 cut(s) 94, 96, 167, 485, 496, 525, 592
FaqI GGGAC 1 cut(s) 118
FatI CATG 1 cut(s) 523
Fnu4HI GCNGC 4 cut(s) 304, 396, 441, 444
FokI GGATG 3 cut(s) 236, 294, 386
Fsp4HI GCNGC 4 cut(s) 304, 396, 441, 444
FspBI CTAG 4 cut(s) 14, 132, 173, 609
GlaI GCGC 1 cut(s) 355
GluI GCNGC 4 cut(s) 304, 396, 441, 444
HaeII RGCGCY 1 cut(s) 357
HaeIII GGCC 2 cut(s) 152, 295
HhaI GCGC 1 cut(s) 356
Hin1II CATG 1 cut(s) 527
Hin6I GCGC 1 cut(s) 354
HinP1I GCGC 1 cut(s) 354
HinfI GANTC 1 cut(s) 380
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 1 cut(s) 204
Hpy188III TCNNGA 1 cut(s) 503
Hpy8I GTNNAC 1 cut(s) 418
HpyAV CCTTC 5 cut(s) 70, 129, 189, 470, 509
HpyCH4V TGCA 5 cut(s) 22, 71, 285, 306, 446
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 437
Hsp92II CATG 1 cut(s) 527
HspAI GCGC 1 cut(s) 354
Kzo9I GATC 2 cut(s) 169, 575
LmnI GCTCC 1 cut(s) 392
Lsp1109I GCAGC 4 cut(s) 290, 407, 430, 452
LweI GCATC 1 cut(s) 272
MaeI CTAG 4 cut(s) 14, 132, 173, 609
MaeIII GTNAC 1 cut(s) 319
MalI GATC 2 cut(s) 171, 577
MboI GATC 2 cut(s) 169, 575
MboII GAAGA 5 cut(s) 56, 172, 518, 544, 570
MfeI CAATTG 1 cut(s) 585
MflI RGATCY 2 cut(s) 169, 575
MlsI TGGCCA 1 cut(s) 295
MluCI AATT 5 cut(s) 248, 467, 480, 535, 585
MluNI TGGCCA 1 cut(s) 295
MnlI CCTC 4 cut(s) 139, 142, 406, 466
Mox20I TGGCCA 1 cut(s) 295
MscI TGGCCA 1 cut(s) 295
MseI TTAA 3 cut(s) 362, 408, 597
Msp20I TGGCCA 1 cut(s) 295
MspA1I CMGCKG 1 cut(s) 443
MspR9I CCNGG 2 cut(s) 297, 317
MunI CAATTG 1 cut(s) 585
MvaI CCWGG 2 cut(s) 297, 317
MwoI GCNNNNNNNGC 2 cut(s) 19, 437
NdeII GATC 2 cut(s) 169, 575
NlaIII CATG 1 cut(s) 527
NmuCI GTSAC 1 cut(s) 319
PfeI GAWTC 1 cut(s) 380
PkrI GCNGC 4 cut(s) 305, 397, 442, 445
Psp6I CCWGG 2 cut(s) 295, 315
PspGI CCWGG 2 cut(s) 295, 315
PstI CTGCAG 1 cut(s) 308
PstNI CAGNNNCTG 3 cut(s) 28, 107, 443
PsuI RGATCY 2 cut(s) 169, 575
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 3 cut(s) 32, 216, 606
RsaNI GTAC 3 cut(s) 31, 215, 605
SaqAI TTAA 3 cut(s) 362, 408, 597
SatI GCNGC 4 cut(s) 304, 396, 441, 444
Sau3AI GATC 2 cut(s) 169, 575
ScaI AGTACT 1 cut(s) 216
ScrFI CCNGG 2 cut(s) 297, 317
SfaNI GCATC 1 cut(s) 272
SfcI CTRYAG 1 cut(s) 304
Sse9I AATT 5 cut(s) 248, 467, 480, 535, 585
SspMI CTAG 4 cut(s) 14, 132, 173, 609
StyD4I CCNGG 2 cut(s) 295, 315
TasI AATT 5 cut(s) 248, 467, 480, 535, 585
TatI WGTACW 2 cut(s) 30, 214
TfiI GAWTC 1 cut(s) 380
Tru1I TTAA 3 cut(s) 362, 408, 597
Tru9I TTAA 3 cut(s) 362, 408, 597
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 4 cut(s) 303, 395, 440, 443
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 2 cut(s) 531, 570
XapI RAATTY 1 cut(s) 535
XspI CTAG 4 cut(s) 14, 132, 173, 609
ZrmI AGTACT 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.