Rh4BG131600

Signal recognition particle 54 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0016992
Physical Location & Seq
Forward (+)
22091196 .. 22129085
37890 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG131600.1

Sequence Viewer

Length: 813 bp
ATGGAACCTACGGAGAGTATGGATAGTTGCGGAGGATTCCACATCGGACCCCATGGGGCAAAACCTTTAAACTTTGGGGCATATGAGGTGGCCATAGCTGTGGACTCTAGACACTTTTTGGCATCCATGATCCACCAGGTACCAAATGGGGCTGCACCAAACATTGCTGTGCCAATACCAATCTCTATAGCTGGAATTCATGCTGGAGCCAACCTACCAGATTTATTCCTCCGTTCCGATTCACCGGCCAACAGAATACTTAAAAGAATTTTTGGGCTTGCTGTTTTAATGGGTGATGCTGCTTCGTATGCAGAGAAGAATTTGTCTTTTAACACAGAAGCAAAACCAGAGAGCAATTCAATTTCATTGGTTCCCCAGACTAAATTTCAAAGCTCAAAGAAGCTGTACAGAAGTTGGGAAGATAATCCGGGTGTTGGTTTTATTGCATTGAAGGTAAAAGTGCCTGTATATACAGCAGGGACTGACACCAAACCTTCACAAATAGCTAGGCAAGGTTTAGAGGAGGCCAAAAAGAAGAACATAGATGTAGTAATAATGGATACTGCTGGAAGGCTTCAGATAGACAAAGTACTGATGGATGAGTTGAAAGAAGTAAAGCGGGAAATGAACCCCACAGAAACTTTACTCGTTGTGGATGCAATGACTGGCCAGGAAGCTGCAGCCCTGGTCACAACATTCAATGTAGAGATTGGGATTACTGGTGCTATTTTAACAAAGCTGGATGGAGATTCTAGAGGTGGAGCAGCTTTGAGTGTTAAAGAGATAACTGAGATGGAAGGCCGGACAACATGA

Protein Analysis

270

Amino Acids

28.92

Weight (kDa)

5.79

Isoelectric Point (pI)

40.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP54 PF00448 148 - 264 1.1e-38 SRP54-type protein, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 139
AccB1I GGYRCC 1 cut(s) 139
AciI CCGC 2 cut(s) 30, 619
AclWI GGATC 1 cut(s) 124
AcoI YGGCCR 3 cut(s) 90, 246, 667
AcsI RAATTY 4 cut(s) 195, 267, 319, 383
AcuI CTGAAG 1 cut(s) 560
AfaI GTAC 3 cut(s) 141, 407, 591
AfiI CCNNNNNNNGG 1 cut(s) 434
AgsI TTSAA 5 cut(s) 360, 389, 451, 607, 700
AjnI CCWGG 3 cut(s) 135, 669, 684
AluBI AGCT 8 cut(s) 98, 191, 393, 403, 506, 677, 739, 767
AluI AGCT 8 cut(s) 98, 191, 393, 403, 506, 677, 739, 767
AlwI GGATC 1 cut(s) 124
AlwNI CAGNNNCTG 1 cut(s) 482
AoxI GGCC 5 cut(s) 90, 246, 525, 667, 799
ApeKI GCWGC 5 cut(s) 152, 299, 677, 680, 764
ApoI RAATTY 4 cut(s) 195, 267, 319, 383
Asp718I GGTACC 1 cut(s) 139
AspS9I GGNCC 1 cut(s) 47
AsuC2I CCSGG 1 cut(s) 429
AsuHPI GGTGA 2 cut(s) 234, 305
AvaII GGWCC 1 cut(s) 47
BalI TGGCCA 2 cut(s) 92, 669
BanI GGYRCC 1 cut(s) 139
BbvI GCAGC 5 cut(s) 139, 286, 664, 692, 776
BccI CCATC 3 cut(s) 589, 737, 787
BciT130I CCWGG 3 cut(s) 137, 671, 686
BciVI GTATCC 1 cut(s) 553
BcnI CCSGG 1 cut(s) 429
BfaI CTAG 3 cut(s) 108, 507, 753
BfmI CTRYAG 2 cut(s) 186, 678
BfuI GTATCC 1 cut(s) 553
BisI GCNGC 5 cut(s) 153, 300, 678, 681, 765
BlsI GCNGC 5 cut(s) 154, 301, 679, 682, 766
BmcAI AGTACT 1 cut(s) 591
Bme1390I CCNGG 4 cut(s) 137, 429, 671, 686
Bme18I GGWCC 1 cut(s) 47
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 5 cut(s) 6, 49, 141, 208, 372
BmrFI CCNGG 4 cut(s) 137, 429, 671, 686
BmsI GCATC 3 cut(s) 131, 286, 646
BpmI CTGGAG 1 cut(s) 225
BpuMI CCSGG 1 cut(s) 429
BsaJI CCNNGG 2 cut(s) 52, 684
Bsc4I CCNNNNNNNGG 1 cut(s) 434
Bse118I RCCGGY 1 cut(s) 244
Bse1I ACTGG 2 cut(s) 670, 724
Bse3DI GCAATG 2 cut(s) 162, 666
BseBI CCWGG 3 cut(s) 137, 671, 686
BseDI CCNNGG 2 cut(s) 52, 684
BseGI GGATG 4 cut(s) 122, 604, 661, 748
BseLI CCNNNNNNNGG 1 cut(s) 434
BseMI GCAATG 2 cut(s) 162, 666
BseMII CTCAG 1 cut(s) 780
BseNI ACTGG 2 cut(s) 670, 724
BseRI GAGGAG 1 cut(s) 536
BseXI GCAGC 5 cut(s) 139, 286, 664, 692, 776
BsgI GTGCAG 1 cut(s) 138
BshFI GGCC 5 cut(s) 92, 248, 527, 669, 801
BshNI GGYRCC 1 cut(s) 139
BsiSI CCGG 3 cut(s) 245, 428, 802
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 1 cut(s) 434
BsmFI GGGAC 1 cut(s) 493
BsnI GGCC 5 cut(s) 92, 248, 527, 669, 801
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 1 cut(s) 129
Bsp19I CCATGG 1 cut(s) 52
BspACI CCGC 2 cut(s) 30, 619
BspANI GGCC 5 cut(s) 92, 248, 527, 669, 801
BspCNI CTCAG 1 cut(s) 781
BspLI GGNNCC 5 cut(s) 6, 49, 141, 208, 372
BspMAI CTGCAG 1 cut(s) 682
BspPI GGATC 1 cut(s) 124
BspT107I GGYRCC 1 cut(s) 139
BsrDI GCAATG 2 cut(s) 162, 666
BsrFI RCCGGY 1 cut(s) 244
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 2 cut(s) 670, 724
BssAI RCCGGY 1 cut(s) 244
BssECI CCNNGG 2 cut(s) 52, 684
BssMI GATC 1 cut(s) 129
BssT1I CCWWGG 1 cut(s) 52
Bst2UI CCWGG 3 cut(s) 137, 671, 686
BstAUI TGTACA 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 1 cut(s) 789
BstDSI CCRYGG 1 cut(s) 52
BstF5I GGATG 4 cut(s) 122, 604, 661, 748
BstKTI GATC 1 cut(s) 132
BstMBI GATC 1 cut(s) 129
BstMWI GCNNNNNNNGC 1 cut(s) 308
BstNI CCWGG 3 cut(s) 137, 671, 686
BstSCI CCNGG 4 cut(s) 135, 427, 669, 684
BstSFI CTRYAG 2 cut(s) 186, 678
BstV1I GCAGC 5 cut(s) 139, 286, 664, 692, 776
BstXI CCANNNNNNTGG 1 cut(s) 100
BsuI GTATCC 1 cut(s) 553
BsuRI GGCC 5 cut(s) 92, 248, 527, 669, 801
BtgI CCRYGG 1 cut(s) 52
BtsCI GGATG 4 cut(s) 122, 604, 661, 748
Cac8I GCNNGC 1 cut(s) 279
CaiI CAGNNNCTG 1 cut(s) 482
Cfr10I RCCGGY 1 cut(s) 244
Cfr13I GGNCC 1 cut(s) 47
CsiI ACCWGGT 1 cut(s) 135
Csp6I GTAC 3 cut(s) 140, 406, 590
CviAII CATG 4 cut(s) 53, 127, 200, 810
CviQI GTAC 3 cut(s) 140, 406, 590
DdeI CTNAG 1 cut(s) 789
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
DraI TTTAAA 1 cut(s) 69
EaeI YGGCCR 3 cut(s) 90, 246, 667
Eco130I CCWWGG 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 560
EcoRI GAATTC 1 cut(s) 195
EcoRII CCWGG 3 cut(s) 135, 669, 684
EcoT14I CCWWGG 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 52
FaeI CATG 4 cut(s) 56, 130, 203, 813
FaqI GGGAC 1 cut(s) 493
FatI CATG 4 cut(s) 52, 126, 199, 809
FauI CCCGC 1 cut(s) 612
FauNDI CATATG 1 cut(s) 82
Fnu4HI GCNGC 5 cut(s) 153, 300, 678, 681, 765
FokI GGATG 4 cut(s) 109, 611, 668, 755
Fsp4HI GCNGC 5 cut(s) 153, 300, 678, 681, 765
FspBI CTAG 3 cut(s) 108, 507, 753
GluI GCNGC 5 cut(s) 153, 300, 678, 681, 765
GsuI CTGGAG 1 cut(s) 225
HaeIII GGCC 5 cut(s) 92, 248, 527, 669, 801
HapII CCGG 3 cut(s) 245, 428, 802
Hin1II CATG 4 cut(s) 56, 130, 203, 813
HinfI GANTC 4 cut(s) 36, 104, 239, 749
HpaII CCGG 3 cut(s) 245, 428, 802
HphI GGTGA 2 cut(s) 234, 305
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 3 cut(s) 47, 238, 579
Hpy188III TCNNGA 2 cut(s) 108, 753
Hpy8I GTNNAC 1 cut(s) 103
HpyAV CCTTC 4 cut(s) 445, 504, 564, 791
HpyCH4V TGCA 5 cut(s) 155, 311, 446, 659, 680
HpyF10VI GCNNNNNNNGC 1 cut(s) 308
HpyF3I CTNAG 1 cut(s) 789
Hsp92II CATG 4 cut(s) 56, 130, 203, 813
KpnI GGTACC 1 cut(s) 143
Kzo9I GATC 1 cut(s) 129
LmnI GCTCC 2 cut(s) 206, 761
Lsp1109I GCAGC 5 cut(s) 139, 286, 664, 692, 776
LweI GCATC 3 cut(s) 131, 286, 646
MabI ACCWGGT 1 cut(s) 135
MaeI CTAG 3 cut(s) 108, 507, 753
MaeIII GTNAC 1 cut(s) 688
MalI GATC 1 cut(s) 131
MboI GATC 1 cut(s) 129
MboII GAAGA 3 cut(s) 328, 431, 547
MlsI TGGCCA 2 cut(s) 92, 669
MluCI AATT 6 cut(s) 195, 267, 319, 355, 360, 383
MluNI TGGCCA 2 cut(s) 92, 669
MlyI GAGTC 1 cut(s) 98
MnlI CCTC 6 cut(s) 26, 79, 239, 514, 517, 749
Mox20I TGGCCA 2 cut(s) 92, 669
MscI TGGCCA 2 cut(s) 92, 669
MseI TTAA 6 cut(s) 68, 261, 287, 330, 731, 777
MslI CAYNNNNRTG 2 cut(s) 98, 167
Msp20I TGGCCA 2 cut(s) 92, 669
MspI CCGG 3 cut(s) 245, 428, 802
MspR9I CCNGG 4 cut(s) 137, 429, 671, 686
MvaI CCWGG 3 cut(s) 137, 671, 686
MwoI GCNNNNNNNGC 1 cut(s) 308
NciI CCSGG 1 cut(s) 429
NcoI CCATGG 1 cut(s) 52
NdeI CATATG 1 cut(s) 82
NdeII GATC 1 cut(s) 129
NlaIII CATG 4 cut(s) 56, 130, 203, 813
NlaIV GGNNCC 5 cut(s) 6, 49, 141, 208, 372
NmuCI GTSAC 1 cut(s) 688
PfeI GAWTC 3 cut(s) 36, 239, 749
PkrI GCNGC 5 cut(s) 154, 301, 679, 682, 766
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
Psp6I CCWGG 3 cut(s) 135, 669, 684
PspGI CCWGG 3 cut(s) 135, 669, 684
PspN4I GGNNCC 5 cut(s) 6, 49, 141, 208, 372
PspPI GGNCC 1 cut(s) 47
PstI CTGCAG 1 cut(s) 682
PstNI CAGNNNCTG 1 cut(s) 482
RsaI GTAC 3 cut(s) 141, 407, 591
RsaNI GTAC 3 cut(s) 140, 406, 590
RseI CAYNNNNRTG 2 cut(s) 98, 167
SaqAI TTAA 6 cut(s) 68, 261, 287, 330, 731, 777
SatI GCNGC 5 cut(s) 153, 300, 678, 681, 765
Sau3AI GATC 1 cut(s) 129
Sau96I GGNCC 1 cut(s) 47
ScaI AGTACT 1 cut(s) 591
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 4 cut(s) 137, 429, 671, 686
SexAI ACCWGGT 1 cut(s) 135
SfaNI GCATC 3 cut(s) 131, 286, 646
SfcI CTRYAG 2 cut(s) 186, 678
SinI GGWCC 1 cut(s) 47
SmiMI CAYNNNNRTG 2 cut(s) 98, 167
Sse9I AATT 6 cut(s) 195, 267, 319, 355, 360, 383
SsiI CCGC 2 cut(s) 30, 619
SspMI CTAG 3 cut(s) 108, 507, 753
StyD4I CCNGG 4 cut(s) 135, 427, 669, 684
StyI CCWWGG 1 cut(s) 52
TasI AATT 6 cut(s) 195, 267, 319, 355, 360, 383
TatI WGTACW 2 cut(s) 405, 589
TfiI GAWTC 3 cut(s) 36, 239, 749
Tru1I TTAA 6 cut(s) 68, 261, 287, 330, 731, 777
Tru9I TTAA 6 cut(s) 68, 261, 287, 330, 731, 777
TseFI GTSAC 1 cut(s) 688
TseI GCWGC 5 cut(s) 152, 299, 677, 680, 764
Tsp45I GTSAC 1 cut(s) 688
TspDTI ATGAA 3 cut(s) 188, 354, 641
TspGWI ACGGA 2 cut(s) 26, 221
VpaK11BI GGWCC 1 cut(s) 47
XapI RAATTY 4 cut(s) 195, 267, 319, 383
XbaI TCTAGA 2 cut(s) 107, 752
XcmI CCANNNNNNNNNTGG 1 cut(s) 143
XspI CTAG 3 cut(s) 108, 507, 753
ZrmI AGTACT 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.