Rroxscaffold_4G00298190

Signal recognition particle 54 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016992
Physical Location & Seq
Reverse (-)
18036375 .. 18039930
3556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00298190.1

Sequence Viewer

Length: 318 bp
ATGGATGAGTTGAAAGAAGTAAAGCGGGAAATGAACCCCACAGAAACTTTACTCGTTGTGGATGCAATGACTGGCCAGGAAGCTGCAGCCCTGGTCACAACATTCAATGTAGAGATTGGGGTTACTGGTGCTATTTTAACAAAGCTGGATGGAGATTCTAGAGGTGGAGCAGCTTTGAGTGTTAAAGAGGTTTACTTGTTGGCTTACTTTTCGTTCATATGTAAAGCAAGGACAGCAGCTGCAGTCAAAGTTCCAGAGTTTCAATTTGAGGTGAGAAGGAATTATAGAGACCACATACCTTCAAGAAGAAGTCAATGA

Protein Analysis

105

Amino Acids

11.65

Weight (kDa)

6.58

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP54 PF00448 1 - 64 5.6e-20 SRP54-type protein, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 25
AcoI YGGCCR 1 cut(s) 73
AgsI TTSAA 4 cut(s) 13, 106, 263, 303
AjnI CCWGG 2 cut(s) 75, 90
AluBI AGCT 4 cut(s) 83, 145, 173, 239
AluI AGCT 4 cut(s) 83, 145, 173, 239
Alw26I GTCTC 1 cut(s) 282
AlwNI CAGNNNCTG 1 cut(s) 239
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 5 cut(s) 83, 86, 170, 236, 239
AsuHPI GGTGA 1 cut(s) 283
BalI TGGCCA 1 cut(s) 75
BbvI GCAGC 5 cut(s) 70, 98, 182, 226, 248
BccI CCATC 1 cut(s) 143
BciT130I CCWGG 2 cut(s) 77, 92
BcoDI GTCTC 1 cut(s) 282
BfaI CTAG 1 cut(s) 159
BfmI CTRYAG 2 cut(s) 84, 240
BisI GCNGC 5 cut(s) 84, 87, 171, 237, 240
BlsI GCNGC 5 cut(s) 85, 88, 172, 238, 241
Bme1390I CCNGG 2 cut(s) 77, 92
BmrFI CCNGG 2 cut(s) 77, 92
BmsI GCATC 1 cut(s) 52
BsaI GGTCTC 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 90
Bse1I ACTGG 2 cut(s) 76, 130
Bse3DI GCAATG 1 cut(s) 72
BseBI CCWGG 2 cut(s) 77, 92
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 3 cut(s) 10, 67, 154
BseMI GCAATG 1 cut(s) 72
BseNI ACTGG 2 cut(s) 76, 130
BseXI GCAGC 5 cut(s) 70, 98, 182, 226, 248
BshFI GGCC 1 cut(s) 75
BsmAI GTCTC 1 cut(s) 282
BsnI GGCC 1 cut(s) 75
Bso31I GGTCTC 1 cut(s) 282
BspACI CCGC 1 cut(s) 25
BspANI GGCC 1 cut(s) 75
BspMAI CTGCAG 2 cut(s) 88, 244
BspTNI GGTCTC 1 cut(s) 282
BsrDI GCAATG 1 cut(s) 72
BsrI ACTGG 2 cut(s) 76, 130
BssECI CCNNGG 1 cut(s) 90
Bst2UI CCWGG 2 cut(s) 77, 92
BstF5I GGATG 3 cut(s) 10, 67, 154
BstMAI GTCTC 1 cut(s) 282
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstNI CCWGG 2 cut(s) 77, 92
BstSCI CCNGG 2 cut(s) 75, 90
BstSFI CTRYAG 2 cut(s) 84, 240
BstV1I GCAGC 5 cut(s) 70, 98, 182, 226, 248
BsuRI GGCC 1 cut(s) 75
BtsCI GGATG 3 cut(s) 10, 67, 154
CaiI CAGNNNCTG 1 cut(s) 239
CviJI RGCY 7 cut(s) 75, 83, 89, 145, 173, 203, 239
CviKI_1 RGCY 7 cut(s) 75, 83, 89, 145, 173, 203, 239
EaeI YGGCCR 1 cut(s) 73
Eco31I GGTCTC 1 cut(s) 282
EcoRII CCWGG 2 cut(s) 75, 90
FaiI YATR 4 cut(s) 218, 220, 285, 296
FauI CCCGC 1 cut(s) 18
FauNDI CATATG 1 cut(s) 218
Fnu4HI GCNGC 5 cut(s) 84, 87, 171, 237, 240
FokI GGATG 3 cut(s) 17, 74, 161
Fsp4HI GCNGC 5 cut(s) 84, 87, 171, 237, 240
FspBI CTAG 1 cut(s) 159
GluI GCNGC 5 cut(s) 84, 87, 171, 237, 240
HaeIII GGCC 1 cut(s) 75
HinfI GANTC 1 cut(s) 155
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 1 cut(s) 193
Hpy188III TCNNGA 3 cut(s) 159, 254, 303
Hpy8I GTNNAC 1 cut(s) 193
HpyAV CCTTC 2 cut(s) 270, 309
HpyCH4V TGCA 3 cut(s) 65, 86, 242
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
LmnI GCTCC 1 cut(s) 167
LpnPI CCDG 8 cut(s) 57, 62, 77, 89, 104, 111, 131, 267
Lsp1109I GCAGC 5 cut(s) 70, 98, 182, 226, 248
LweI GCATC 1 cut(s) 52
MaeI CTAG 1 cut(s) 159
MaeIII GTNAC 2 cut(s) 94, 121
MboII GAAGA 1 cut(s) 318
MlsI TGGCCA 1 cut(s) 75
MluCI AATT 2 cut(s) 263, 280
MluNI TGGCCA 1 cut(s) 75
MnlI CCTC 3 cut(s) 155, 181, 262
Mox20I TGGCCA 1 cut(s) 75
MscI TGGCCA 1 cut(s) 75
MseI TTAA 2 cut(s) 137, 183
Msp20I TGGCCA 1 cut(s) 75
MspA1I CMGCKG 1 cut(s) 239
MspR9I CCNGG 2 cut(s) 77, 92
MvaI CCWGG 2 cut(s) 77, 92
MwoI GCNNNNNNNGC 1 cut(s) 233
NdeI CATATG 1 cut(s) 218
NmuCI GTSAC 1 cut(s) 94
PfeI GAWTC 1 cut(s) 155
PkrI GCNGC 5 cut(s) 85, 88, 172, 238, 241
Psp6I CCWGG 2 cut(s) 75, 90
PspGI CCWGG 2 cut(s) 75, 90
PstI CTGCAG 2 cut(s) 88, 244
PstNI CAGNNNCTG 1 cut(s) 239
PvuII CAGCTG 1 cut(s) 239
SaqAI TTAA 2 cut(s) 137, 183
SatI GCNGC 5 cut(s) 84, 87, 171, 237, 240
ScrFI CCNGG 2 cut(s) 77, 92
SetI ASST 8 cut(s) 85, 147, 166, 175, 192, 241, 273, 301
SfaNI GCATC 1 cut(s) 52
SfcI CTRYAG 2 cut(s) 84, 240
Sse9I AATT 2 cut(s) 263, 280
SsiI CCGC 1 cut(s) 25
SspMI CTAG 1 cut(s) 159
StyD4I CCNGG 2 cut(s) 75, 90
TasI AATT 2 cut(s) 263, 280
TfiI GAWTC 1 cut(s) 155
Tru1I TTAA 2 cut(s) 137, 183
Tru9I TTAA 2 cut(s) 137, 183
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 5 cut(s) 83, 86, 170, 236, 239
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 2 cut(s) 47, 205
XbaI TCTAGA 1 cut(s) 158
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.