RchiOBHm_Chr2g0137681

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004864
Physical Location & Seq
Reverse (-)
55480740 .. 55483305
2566 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 912 bp
ATGGCGCCTTCACTAACTATTGAGAAAGAATTTGCTCAAACCTCAGAAAGAGTGAAGTCTGTGGATCTGCATCCAACTGAGCCATGGATGCTAGCAAGTTTGTATTCAGGAACTATTTGTATCTGGAACTACCAATCACAGACCCTGGAGAAGTCCATCAAAGTCACTGAGTCACCAGTCAGATCAGTAAAGTTTGTAGCTCGCAAACACTGGATTATAACTGGGGCTGATGACAAGTTCATTCGCGTATACAACTACAATACCATGGAAAAGATCAAAGAGTATGAAGCACATACTGATTATATCAGGAGTGTCACTGTCCATCCAACTCTACCATATCTACTGTCATGTTCTGATGACAAGGTTATAAAGCTTTGGGACTGGGAGAAGGACTGGGAATGCACTCAGGTCTTTGAGGGGCATTCCCACTATGTGATGCAAGTGGCTTTCAATCCGAAAGACACCAATAGTTTTGCTAGTGCGTCTCTCGATGGAACCATTAAGACATGGAACATTGGCTCATCTGCCCCAAATTTTACATTACAAGGCCACTCAAAAGGAGTGAATTGTGTTGATTATTTCGTTCACAGCGATAAGACTTACCTGTTAAGTGGTTCTGATGACTTCACTGCCAAGGTATGGGACTATGAAACTACAAGTTGTGTGCAAACTCTAGAAGGTCATGAGAACAATGTAACAACTGTGAGTGTACATCCTGAGCTTCCCATAATAATTACAACTTCTGAGGATGGGACTGTTCATATATGGAATGCAACCACATTTAGTCTAGAGCACAAACTGAACTATGGTCTTGAAAGAGTTTGGGCCATTGGACACAAGAAAGGATCATACAAGGTTGCATTTGGTTTTGACAATGGAACCACTATAGTCAAAATGAATGCTTCTGCTTGA

Protein Analysis

303

Amino Acids

34.37

Weight (kDa)

5.89

Isoelectric Point (pI)

27.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 3 - 114 1.3e-11 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 10 - 154 1.3e-19 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 10 - 175 5e-14 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 13 - 171 1.1e-17 WDR3 second beta-propeller domain
EIF3I PF24805 13 - 112 1.2e-06 EIF3I
WDR55 PF24796 14 - 199 9.1e-20 WDR55
WD40_Prp19 PF24814 14 - 137 1.1e-17 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 20 - 173 1.1e-18 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 35 - 225 9.2e-25 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 35 - 196 1.4e-13 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 55 - 173 1e-25 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 57 - 131 1e-10 WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd PF24782 65 - 171 5.5e-10 MABP1/WDR62 second WD40 domain
Beta-prop_WDR75_1st PF23869 76 - 129 6.2e-06 WD repeat-containing protein 75 first beta-propeller
WD40 PF00400 90 - 127 7.5e-09 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 98 - 190 1.8e-16 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 98 - 173 7.4e-06 TEP-1 second beta-propeller
WD40 PF00400 132 - 171 1.7e-07 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 132 - 284 2.3e-28 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 134 - 275 1.1e-21 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 134 - 184 1.8e-07 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 154 - 280 1.5e-27 WDR5 beta-propeller domain
WD40_Gbeta PF25391 154 - 257 1.6e-11 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 156 - 292 3.6e-14 WDR3 second beta-propeller domain
Beta-prop_SCAP PF24017 161 - 260 5e-08 SCAP Beta-propeller
WD40_MABP1-WDR62_2nd PF24782 161 - 257 4.1e-07 MABP1/WDR62 second WD40 domain
Beta-prop_EML PF23409 166 - 266 3.4e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 177 - 215 7.3e-10 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 180 - 267 4e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR41 PF25178 181 - 260 3.9e-06 WDR41
EIF3I PF24805 181 - 260 1.5e-06 EIF3I
WDR55 PF24796 183 - 295 2.8e-10 WDR55
Beta-prop_THOC3 PF25174 184 - 273 2.9e-14 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 188 - 294 2.8e-15 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 194 - 260 3.9e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR75_1st PF23869 196 - 259 9.7e-08 WD repeat-containing protein 75 first beta-propeller
WD40_CDC20-Fz PF24807 197 - 267 1.2e-06 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 198 - 260 2.1e-07 TEP-1 second beta-propeller
WD40 PF00400 220 - 257 9.1e-10 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 234 - 297 5.4e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 218, 368
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 639
AccI GTMKAC 1 cut(s) 249
AccII CGCG 1 cut(s) 246
AclWI GGATC 2 cut(s) 72, 853
AcsI RAATTY 2 cut(s) 29, 532
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 433
AfaI GTAC 1 cut(s) 711
AfiI CCNNNNNNNGG 1 cut(s) 639
AgsI TTSAA 2 cut(s) 451, 815
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 3 cut(s) 200, 373, 721
AluI AGCT 3 cut(s) 200, 373, 721
Alw21I GWGCWC 1 cut(s) 795
Alw26I GTCTC 1 cut(s) 489
AlwI GGATC 2 cut(s) 72, 853
AlwNI CAGNNNCTG 1 cut(s) 145
AoxI GGCC 2 cut(s) 547, 825
ApoI RAATTY 2 cut(s) 29, 532
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 825
AsuHPI GGTGA 1 cut(s) 165
AsuNHI GCTAGC 1 cut(s) 91
BanI GGYRCC 1 cut(s) 4
Bbv12I GWGCWC 1 cut(s) 795
BccI CCATC 4 cut(s) 164, 330, 485, 743
BciT130I CCWGG 1 cut(s) 146
BcoDI GTCTC 1 cut(s) 489
BfaI CTAG 4 cut(s) 92, 477, 674, 788
BfmI CTRYAG 1 cut(s) 885
BfoI RGCGCY 1 cut(s) 8
Bme1390I CCNGG 1 cut(s) 146
BmgT120I GGNCC 1 cut(s) 825
BmiI GGNNCC 3 cut(s) 6, 496, 880
BmrFI CCNGG 1 cut(s) 146
BmrI ACTGGG 3 cut(s) 231, 391, 403
BmsI GCATC 3 cut(s) 78, 79, 426
BmtI GCTAGC 1 cut(s) 95
BmuI ACTGGG 3 cut(s) 231, 391, 403
BpmI CTGGAG 1 cut(s) 167
Bpu10I CCTNAGC 1 cut(s) 717
BsaBI GATNNNNATC 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 4 cut(s) 83, 144, 264, 633
Bsc4I CCNNNNNNNGG 1 cut(s) 639
Bse1I ACTGG 5 cut(s) 176, 215, 226, 386, 398
Bse8I GATNNNNATC 1 cut(s) 69
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 4 cut(s) 83, 144, 264, 633
BseGI GGATG 5 cut(s) 70, 93, 322, 712, 754
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 1 cut(s) 639
BseMII CTCAG 6 cut(s) 57, 69, 159, 419, 708, 735
BseNI ACTGG 5 cut(s) 176, 215, 226, 386, 398
Bsh1236I CGCG 1 cut(s) 246
BshFI GGCC 2 cut(s) 549, 827
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 795
BslFI GGGAC 3 cut(s) 392, 656, 766
BslI CCNNNNNNNGG 1 cut(s) 639
BsmAI GTCTC 1 cut(s) 489
BsmBI CGTCTC 1 cut(s) 489
BsmFI GGGAC 3 cut(s) 392, 656, 766
BsmI GAATGC 4 cut(s) 404, 421, 775, 904
BsnI GGCC 2 cut(s) 549, 827
Bsp1286I GDGCHC 1 cut(s) 795
Bsp1407I TGTACA 1 cut(s) 709
Bsp143I GATC 4 cut(s) 64, 182, 273, 845
Bsp19I CCATGG 2 cut(s) 83, 264
BspANI GGCC 2 cut(s) 549, 827
BspCNI CTCAG 6 cut(s) 56, 70, 160, 418, 709, 736
BspFNI CGCG 1 cut(s) 246
BspHI TCATGA 1 cut(s) 682
BspLI GGNNCC 3 cut(s) 6, 496, 880
BspOI GCTAGC 1 cut(s) 95
BspPI GGATC 2 cut(s) 72, 853
BspT107I GGYRCC 1 cut(s) 4
BsrGI TGTACA 1 cut(s) 709
BsrI ACTGG 5 cut(s) 176, 215, 226, 386, 398
BssECI CCNNGG 4 cut(s) 83, 144, 264, 633
BssMI GATC 4 cut(s) 64, 182, 273, 845
BssNAI GTATAC 1 cut(s) 250
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 83, 264, 633
Bst1107I GTATAC 1 cut(s) 250
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 4 cut(s) 319, 345, 703, 757
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 709
BstC8I GCNNGC 2 cut(s) 93, 202
BstDEI CTNAG 6 cut(s) 43, 78, 168, 405, 717, 744
BstDSI CCRYGG 2 cut(s) 83, 264
BstF5I GGATG 5 cut(s) 70, 93, 322, 712, 754
BstFNI CGCG 1 cut(s) 246
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 4 cut(s) 67, 185, 276, 848
BstMAI GTCTC 1 cut(s) 489
BstMBI GATC 4 cut(s) 64, 182, 273, 845
BstMWI GCNNNNNNNGC 1 cut(s) 88
BstNI CCWGG 1 cut(s) 146
BstSCI CCNGG 1 cut(s) 144
BstSFI CTRYAG 1 cut(s) 885
BstUI CGCG 1 cut(s) 246
BstX2I RGATCY 1 cut(s) 64
BstYI RGATCY 1 cut(s) 64
BstZ17I GTATAC 1 cut(s) 250
BsuRI GGCC 2 cut(s) 549, 827
BtgI CCRYGG 2 cut(s) 83, 264
BtsCI GGATG 5 cut(s) 70, 93, 322, 712, 754
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 4 cut(s) 165, 208, 315, 627
Cac8I GCNNGC 2 cut(s) 93, 202
CaiI CAGNNNCTG 1 cut(s) 145
CciI TCATGA 1 cut(s) 682
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 825
CseI GACGC 1 cut(s) 471
Csp6I GTAC 1 cut(s) 710
CviAII CATG 5 cut(s) 84, 265, 348, 507, 683
CviJI RGCY 9 cut(s) 82, 200, 227, 373, 446, 519, 549, 721, 827
CviKI_1 RGCY 9 cut(s) 82, 200, 227, 373, 446, 519, 549, 721, 827
CviQI GTAC 1 cut(s) 710
DdeI CTNAG 6 cut(s) 43, 78, 168, 405, 717, 744
DinI GGCGCC 1 cut(s) 6
DpnI GATC 4 cut(s) 66, 184, 275, 847
DpnII GATC 4 cut(s) 64, 182, 273, 845
DraIII CACNNNGTG 1 cut(s) 433
Eco130I CCWWGG 3 cut(s) 83, 264, 633
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 3 cut(s) 83, 264, 633
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 83, 264, 633
Esp3I CGTCTC 1 cut(s) 489
FaeI CATG 5 cut(s) 87, 268, 351, 510, 686
FaqI GGGAC 3 cut(s) 392, 656, 766
FatI CATG 5 cut(s) 83, 264, 347, 506, 682
FblI GTMKAC 1 cut(s) 249
FokI GGATG 5 cut(s) 57, 100, 309, 699, 761
FspBI CTAG 4 cut(s) 92, 477, 674, 788
GlaI GCGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 167
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 549, 827
HgaI GACGC 1 cut(s) 471
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 87, 268, 351, 510, 686
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 1 cut(s) 170
HphI GGTGA 1 cut(s) 165
Hpy166II GTNNAC 3 cut(s) 250, 586, 710
Hpy188I TCNGA 6 cut(s) 46, 182, 355, 456, 619, 745
Hpy188III TCNNGA 9 cut(s) 108, 124, 307, 488, 674, 683, 716, 788, 812
Hpy8I GTNNAC 3 cut(s) 250, 586, 710
HpyAV CCTTC 3 cut(s) 18, 382, 671
HpyCH4III ACNGT 4 cut(s) 319, 345, 703, 757
HpyCH4V TGCA 6 cut(s) 70, 402, 439, 667, 773, 860
HpyF10VI GCNNNNNNNGC 1 cut(s) 88
HpyF3I CTNAG 6 cut(s) 43, 78, 168, 405, 717, 744
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 87, 268, 351, 510, 686
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 4 cut(s) 64, 182, 273, 845
LweI GCATC 3 cut(s) 78, 79, 426
MaeI CTAG 4 cut(s) 92, 477, 674, 788
MaeIII GTNAC 4 cut(s) 163, 171, 313, 694
MalI GATC 4 cut(s) 66, 184, 275, 847
MboI GATC 4 cut(s) 64, 182, 273, 845
MflI RGATCY 1 cut(s) 64
MhlI GDGCHC 1 cut(s) 795
MluCI AATT 4 cut(s) 29, 532, 565, 732
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 2 cut(s) 98, 350
MnlI CCTC 3 cut(s) 52, 409, 739
MseI TTAA 2 cut(s) 501, 608
MspR9I CCNGG 1 cut(s) 146
Mva1269I GAATGC 4 cut(s) 404, 421, 775, 904
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 1 cut(s) 246
MwoI GCNNNNNNNGC 1 cut(s) 88
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 2 cut(s) 83, 264
NdeII GATC 4 cut(s) 64, 182, 273, 845
NheI GCTAGC 1 cut(s) 91
NlaIII CATG 5 cut(s) 87, 268, 351, 510, 686
NlaIV GGNNCC 3 cut(s) 6, 496, 880
NmuCI GTSAC 3 cut(s) 163, 171, 313
PagI TCATGA 1 cut(s) 682
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PctI GAATGC 4 cut(s) 404, 421, 775, 904
PflMI CCANNNNNTGG 1 cut(s) 639
PleI GAGTC 1 cut(s) 178
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 178
PsiI TTATAA 2 cut(s) 218, 368
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 3 cut(s) 6, 496, 880
PspPI GGNCC 1 cut(s) 825
PstNI CAGNNNCTG 1 cut(s) 145
PsuI RGATCY 1 cut(s) 64
RsaI GTAC 1 cut(s) 711
RsaNI GTAC 1 cut(s) 710
SaqAI TTAA 2 cut(s) 501, 608
Sau3AI GATC 4 cut(s) 64, 182, 273, 845
Sau96I GGNCC 1 cut(s) 825
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 1 cut(s) 146
SduI GDGCHC 1 cut(s) 795
SfaNI GCATC 3 cut(s) 78, 79, 426
SfcI CTRYAG 1 cut(s) 885
SfoI GGCGCC 1 cut(s) 6
Sse9I AATT 4 cut(s) 29, 532, 565, 732
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 4 cut(s) 92, 477, 674, 788
StyD4I CCNGG 1 cut(s) 144
StyI CCWWGG 3 cut(s) 83, 264, 633
TaaI ACNGT 4 cut(s) 319, 345, 703, 757
TaqI TCGA 1 cut(s) 489
TasI AATT 4 cut(s) 29, 532, 565, 732
TatI WGTACW 1 cut(s) 709
Tru1I TTAA 2 cut(s) 501, 608
Tru9I TTAA 2 cut(s) 501, 608
TscAI CASTG 4 cut(s) 172, 215, 322, 634
TseFI GTSAC 3 cut(s) 163, 171, 313
Tsp45I GTSAC 3 cut(s) 163, 171, 313
TspDTI ATGAA 5 cut(s) 229, 300, 663, 749, 911
TspRI CASTG 4 cut(s) 172, 215, 322, 634
Van91I CCANNNNNTGG 1 cut(s) 639
XapI RAATTY 2 cut(s) 29, 532
XbaI TCTAGA 2 cut(s) 673, 787
XcmI CCANNNNNNNNNTGG 1 cut(s) 81
XmiI GTMKAC 1 cut(s) 249
XspI CTAG 4 cut(s) 92, 477, 674, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.