RchiOBHm_Chr1g0355111

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0009803
Physical Location & Seq
Reverse (-)
47858412 .. 47860805
2394 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1080 bp
ATGGAACAGCCTGGTAATGGAACAGATGGGATCAGTCAAATGGAACCGGCCCAACAGCCACTGATATCTGGACTTCCTGATGACATTGCGCTTTTCTGCATGGCAAGGGTTCCCCGAAAGTACCATACAGTCCTAAAGTGTGTTTCAAAAAGATGGCAACACTTAGTGGGCAGTGAAGAGTGGCATTCCTACCGTCGAAAGCATAATCTTGATGAGACTTGGATCTATGCTTTGTGCCGAGACAAACTGGAGCGTCTTTGCTTGTATGTATTGGATCCAAAGTCATCAAGAAGGAGTTGGAAGCTTGTTAATGAGCTTCCTCCTCGCACCTTGAAGAGAAAAGGCATGGGTTTTGAAGTGCTAGGAAAGAAAGTTTACTTGTTGGGTGGTTGCGGTTGGTGTGAAGATGCTACAGATGAGGTCTACTACTATGATGCTTCCCTGAACATGTGGAGTGAAGCTACTCCCTTATCTATTGCTAGGTGCTATTTTGCCTGTGAAGTATTAGATGAGAAAATATATTCCATTGGTGGATTAGGTTCAAATTCAAGTGATCCACATTCTTGGGACATTTATGATCCTAGCACTAATAGTTGGATATTTCACTCAGACCCAAACATTGTCCCTGAAATTGAGGATTCTGTGGTCATGGATGGGAAAATCTACATCCGCTGTGGCACTTCTGCTGTATCTTCTCATGTCTATGCTGTTGTTTATGAACCATCAAGCGGTACATGGCAGCATGCAGATGCTGATATGGTTTCTGGCTGGCGAGGTCCGGCAGTTGTAATAGACGAGACCCTCTATGTATTGGATCAGAGCTCAGGAACTAGGCTGATGATGTGGCAGAAGGAGACTAGGGAATGGATTCCAGTTGGGAGGCTGTCATCCTTGCTTACCAGACCACCCTGTCAACTCGTAGCTATTGGGAAGAGTATTTATGTTGTCGGCAAGGGGCTTAGCACAGTCATGTTTGATGTTGAAAATGCAGGGAATATGGAAGGGGTAATTGTGAGTTCTTCAATACCTAAATTGAATTCTGATGATGATGTAATCAGCTGTAAATGTTTGTCCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

359

Amino Acids

40.31

Weight (kDa)

5.45

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 60 4.5e-07 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 87 - 199 1.1e-07 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 88 - 250 1.5e-09 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 93 - 303 1.5e-26 FKB95, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 96 - 270 7.8e-08 Attractin/LZTR1 beta-propeller
Beta-prop_Calicin PF13964 112 - 275 6.3e-09 Calicin, beta-propeller domain
Kelch_1 PF01344 160 - 199 7.2e-07 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 909
AccI GTMKAC 1 cut(s) 423
AciI CCGC 3 cut(s) 393, 670, 729
AclWI GGATC 7 cut(s) 38, 230, 269, 282, 548, 572, 822
AcsI RAATTY 2 cut(s) 544, 1036
AdeI CACNNNGTG 1 cut(s) 166
AfaI GTAC 2 cut(s) 122, 733
AfiI CCNNNNNNNGG 2 cut(s) 17, 728
AflIII ACRYGT 1 cut(s) 447
AgsI TTSAA 8 cut(s) 147, 334, 356, 543, 549, 983, 1023, 1036
AjnI CCWGG 1 cut(s) 10
AluBI AGCT 6 cut(s) 304, 316, 461, 822, 923, 1059
AluI AGCT 6 cut(s) 304, 316, 461, 822, 923, 1059
Alw21I GWGCWC 1 cut(s) 824
Alw26I GTCTC 4 cut(s) 209, 234, 791, 848
AlwI GGATC 7 cut(s) 38, 230, 269, 282, 548, 572, 822
AlwNI CAGNNNCTG 2 cut(s) 61, 752
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 1 cut(s) 739
ApoI RAATTY 2 cut(s) 544, 1036
Asp700I GAANNNNTTC 1 cut(s) 867
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 2 cut(s) 49, 776
AvaII GGWCC 1 cut(s) 776
BamHI GGATCC 1 cut(s) 274
BanII GRGCYC 1 cut(s) 824
Bbv12I GWGCWC 1 cut(s) 824
BbvI GCAGC 1 cut(s) 751
BccI CCATC 4 cut(s) 20, 147, 647, 730
BciT130I CCWGG 1 cut(s) 12
BcoDI GTCTC 4 cut(s) 209, 234, 791, 848
BfaI CTAG 5 cut(s) 362, 480, 582, 831, 858
BfmI CTRYAG 1 cut(s) 411
BisI GCNGC 1 cut(s) 740
BlpI GCTNAGC 1 cut(s) 959
BlsI GCNGC 1 cut(s) 741
Bme1390I CCNGG 1 cut(s) 12
Bme18I GGWCC 1 cut(s) 776
BmgT120I GGNCC 2 cut(s) 49, 776
BmiI GGNNCC 3 cut(s) 45, 111, 276
BmrFI CCNGG 1 cut(s) 12
BmsI GCATC 3 cut(s) 397, 424, 739
BpmI CTGGAG 1 cut(s) 269
Bpu10I CCTNAGC 1 cut(s) 823
Bpu1102I GCTNAGC 1 cut(s) 959
BsaI GGTCTC 1 cut(s) 791
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 728
Bse118I RCCGGY 1 cut(s) 46
Bse1I ACTGG 2 cut(s) 252, 872
Bse3DI GCAATG 1 cut(s) 84
BseBI CCWGG 1 cut(s) 12
BseGI GGATG 3 cut(s) 658, 666, 887
BseLI CCNNNNNNNGG 2 cut(s) 17, 728
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 2 cut(s) 621, 837
BseNI ACTGG 2 cut(s) 252, 872
BseRI GAGGAG 1 cut(s) 312
BseXI GCAGC 1 cut(s) 751
BshFI GGCC 1 cut(s) 50
BsiHKAI GWGCWC 1 cut(s) 824
BsiSI CCGG 2 cut(s) 47, 779
BslFI GGGAC 2 cut(s) 581, 608
BslI CCNNNNNNNGG 2 cut(s) 17, 728
BsmAI GTCTC 4 cut(s) 209, 234, 791, 848
BsmFI GGGAC 2 cut(s) 581, 608
BsmI GAATGC 1 cut(s) 184
BsnI GGCC 1 cut(s) 50
Bso31I GGTCTC 1 cut(s) 791
Bsp1286I GDGCHC 1 cut(s) 824
Bsp143I GATC 6 cut(s) 30, 222, 274, 553, 577, 814
Bsp1720I GCTNAGC 1 cut(s) 959
BspACI CCGC 3 cut(s) 393, 670, 729
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 2 cut(s) 620, 836
BspLI GGNNCC 3 cut(s) 45, 111, 276
BspPI GGATC 7 cut(s) 38, 230, 269, 282, 548, 572, 822
BspTNI GGTCTC 1 cut(s) 791
BsrDI GCAATG 1 cut(s) 84
BsrFI RCCGGY 1 cut(s) 46
BsrI ACTGG 2 cut(s) 252, 872
BssAI RCCGGY 1 cut(s) 46
BssMI GATC 6 cut(s) 30, 222, 274, 553, 577, 814
Bst2UI CCWGG 1 cut(s) 12
Bst4CI ACNGT 3 cut(s) 130, 194, 967
Bst6I CTCTTC 3 cut(s) 171, 329, 926
BstC8I GCNNGC 2 cut(s) 744, 770
BstDEI CTNAG 4 cut(s) 163, 607, 823, 959
BstF5I GGATG 3 cut(s) 658, 666, 887
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 6 cut(s) 33, 225, 277, 556, 580, 817
BstMAI GTCTC 4 cut(s) 209, 234, 791, 848
BstMBI GATC 6 cut(s) 30, 222, 274, 553, 577, 814
BstNI CCWGG 1 cut(s) 12
BstNSI RCATGY 2 cut(s) 451, 746
BstSCI CCNGG 1 cut(s) 10
BstSFI CTRYAG 1 cut(s) 411
BstV1I GCAGC 1 cut(s) 751
BstX2I RGATCY 2 cut(s) 222, 274
BstXI CCANNNNNNTGG 1 cut(s) 564
BstYI RGATCY 2 cut(s) 222, 274
BsuRI GGCC 1 cut(s) 50
BtsCI GGATG 3 cut(s) 658, 666, 887
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 2 cut(s) 59, 178
Cac8I GCNNGC 2 cut(s) 744, 770
CaiI CAGNNNCTG 2 cut(s) 61, 752
CfoI GCGC 1 cut(s) 91
Cfr10I RCCGGY 1 cut(s) 46
Cfr13I GGNCC 2 cut(s) 49, 776
CseI GACGC 1 cut(s) 242
Csp6I GTAC 2 cut(s) 121, 732
CviAII CATG 8 cut(s) 100, 346, 448, 649, 698, 735, 743, 970
CviQI GTAC 2 cut(s) 121, 732
DdeI CTNAG 4 cut(s) 163, 607, 823, 959
DpnI GATC 6 cut(s) 32, 224, 276, 555, 579, 816
DpnII GATC 6 cut(s) 30, 222, 274, 553, 577, 814
DraIII CACNNNGTG 1 cut(s) 166
DrdI GACNNNNNNGTC 1 cut(s) 909
DseDI GACNNNNNNGTC 1 cut(s) 909
Eam1104I CTCTTC 3 cut(s) 171, 329, 926
EarI CTCTTC 3 cut(s) 171, 329, 926
Ecl136II GAGCTC 1 cut(s) 822
Eco24I GRGCYC 1 cut(s) 824
Eco31I GGTCTC 1 cut(s) 791
Eco32I GATATC 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 776
Eco53kI GAGCTC 1 cut(s) 822
EcoICRI GAGCTC 1 cut(s) 822
EcoRI GAATTC 1 cut(s) 1036
EcoRII CCWGG 1 cut(s) 10
EcoRV GATATC 1 cut(s) 66
EcoT38I GRGCYC 1 cut(s) 824
FaeI CATG 8 cut(s) 103, 349, 451, 652, 701, 738, 746, 973
FaqI GGGAC 2 cut(s) 581, 608
FatI CATG 8 cut(s) 99, 345, 447, 648, 697, 734, 742, 969
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 1 cut(s) 740
FokI GGATG 3 cut(s) 653, 665, 874
FriOI GRGCYC 1 cut(s) 824
Fsp4HI GCNGC 1 cut(s) 740
FspBI CTAG 5 cut(s) 362, 480, 582, 831, 858
GlaI GCGC 1 cut(s) 90
GluI GCNGC 1 cut(s) 740
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 1 cut(s) 50
HapII CCGG 2 cut(s) 47, 779
HgaI GACGC 1 cut(s) 242
HhaI GCGC 1 cut(s) 91
Hin1II CATG 8 cut(s) 103, 349, 451, 652, 701, 738, 746, 973
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 914
HindII GTYRAC 1 cut(s) 914
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 2 cut(s) 638, 868
HpaII CCGG 2 cut(s) 47, 779
Hpy166II GTNNAC 3 cut(s) 376, 424, 914
Hpy188I TCNGA 3 cut(s) 610, 819, 1042
Hpy188III TCNNGA 5 cut(s) 69, 77, 209, 288, 825
Hpy8I GTNNAC 3 cut(s) 376, 424, 914
Hpy99I CGWCG 1 cut(s) 198
HpyAV CCTTC 3 cut(s) 285, 844, 995
HpyCH4III ACNGT 3 cut(s) 130, 194, 967
HpyCH4V TGCA 3 cut(s) 99, 746, 989
HpyF3I CTNAG 4 cut(s) 163, 607, 823, 959
Hsp92II CATG 8 cut(s) 103, 349, 451, 652, 701, 738, 746, 973
HspAI GCGC 1 cut(s) 89
Kzo9I GATC 6 cut(s) 30, 222, 274, 553, 577, 814
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 1 cut(s) 751
LweI GCATC 3 cut(s) 397, 424, 739
MaeI CTAG 5 cut(s) 362, 480, 582, 831, 858
MalI GATC 6 cut(s) 32, 224, 276, 555, 579, 816
MboI GATC 6 cut(s) 30, 222, 274, 553, 577, 814
MboII GAAGA 6 cut(s) 188, 346, 416, 684, 943, 1011
MflI RGATCY 2 cut(s) 222, 274
MhlI GDGCHC 1 cut(s) 824
MluCI AATT 5 cut(s) 544, 630, 1008, 1031, 1036
MmeI TCCRAC 2 cut(s) 278, 575
MnlI CCTC 7 cut(s) 330, 333, 412, 628, 767, 812, 873
MroXI GAANNNNTTC 1 cut(s) 867
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 3 cut(s) 702, 747, 968
MspA1I CMGCKG 2 cut(s) 672, 1059
MspI CCGG 2 cut(s) 47, 779
MspR9I CCNGG 1 cut(s) 12
Mva1269I GAATGC 1 cut(s) 184
MvaI CCWGG 1 cut(s) 12
NdeII GATC 6 cut(s) 30, 222, 274, 553, 577, 814
NlaIII CATG 8 cut(s) 103, 349, 451, 652, 701, 738, 746, 973
NlaIV GGNNCC 3 cut(s) 45, 111, 276
NmeAIII GCCGAG 1 cut(s) 263
NspI RCATGY 2 cut(s) 451, 746
PaeI GCATGC 1 cut(s) 746
PciI ACATGT 1 cut(s) 447
PctI GAATGC 1 cut(s) 184
PdmI GAANNNNTTC 1 cut(s) 867
PfeI GAWTC 2 cut(s) 638, 868
PkrI GCNGC 1 cut(s) 741
PscI ACATGT 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 824
Psp6I CCWGG 1 cut(s) 10
PspGI CCWGG 1 cut(s) 10
PspN4I GGNNCC 3 cut(s) 45, 111, 276
PspPI GGNCC 2 cut(s) 49, 776
PstNI CAGNNNCTG 2 cut(s) 61, 752
PsuI RGATCY 2 cut(s) 222, 274
PvuII CAGCTG 1 cut(s) 1059
RsaI GTAC 2 cut(s) 122, 733
RsaNI GTAC 2 cut(s) 121, 732
RseI CAYNNNNRTG 3 cut(s) 702, 747, 968
SacI GAGCTC 1 cut(s) 824
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 1 cut(s) 740
Sau3AI GATC 6 cut(s) 30, 222, 274, 553, 577, 814
Sau96I GGNCC 2 cut(s) 49, 776
ScrFI CCNGG 1 cut(s) 12
SduI GDGCHC 1 cut(s) 824
SfaNI GCATC 3 cut(s) 397, 424, 739
SfcI CTRYAG 1 cut(s) 411
SinI GGWCC 1 cut(s) 776
SmiMI CAYNNNNRTG 3 cut(s) 702, 747, 968
SphI GCATGC 1 cut(s) 746
Sse9I AATT 5 cut(s) 544, 630, 1008, 1031, 1036
SsiI CCGC 3 cut(s) 393, 670, 729
SspMI CTAG 5 cut(s) 362, 480, 582, 831, 858
SstI GAGCTC 1 cut(s) 824
StyD4I CCNGG 1 cut(s) 10
TaaI ACNGT 3 cut(s) 130, 194, 967
TaqI TCGA 1 cut(s) 196
TasI AATT 5 cut(s) 544, 630, 1008, 1031, 1036
TfiI GAWTC 2 cut(s) 638, 868
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 2 cut(s) 66, 178
TseI GCWGC 1 cut(s) 739
TspDTI ATGAA 1 cut(s) 732
TspRI CASTG 2 cut(s) 66, 178
VpaK11BI GGWCC 1 cut(s) 776
XapI RAATTY 2 cut(s) 544, 1036
XceI RCATGY 2 cut(s) 451, 746
XmiI GTMKAC 1 cut(s) 423
XmnI GAANNNNTTC 1 cut(s) 867
XspI CTAG 5 cut(s) 362, 480, 582, 831, 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.