RchiOBHm_Chr1g0377651

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0007825
Physical Location & Seq
Reverse (-)
64453980 .. 64462979
9000 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1263 bp
ATGGTTACTGAAATGAAGACGACGATGACGAAGAGCGTGAATGCGGTGGTAGCAGTCACGTTGGCGCTGCTAACGTGCGCGCCTTGGTGCATTTACTCACAGCAGCCGCACCAGCTTATGGTGAACATGACCACCGTCCGAAATGCCCCCGCTCTCGGCGCTTTTTGCTTGGATGGGAGTTTGCCTGCGTATCATATTCACAGAGGCTTCGGCGCCGGAGCAAGAAACTGGCTTTTACAGTTTGAGGGTGGAGGGTGGTGTAATGATATAGACTCTTGCTTGGAGAGGGCGAAAACAAGAAGAGGTTCTACACACCTTATGAGCAAGTGGGAGGTCTTTTCTGGGATTCTAAGTAATAATGCTTCTCTTAATCCAGATTTTTACAATTGGAACCGTGTGAAGATTCGATATTGTGATGGAGCTTCATTTGCCGGAGACGCAGTGTATAAGAATGGGACAACGTTGCTCTATTTCAGAGGGCAAAAGATTTGGGAAGCCATCATTCTTGATCTTCTGCCTAAAGGTTTAGGAAAGGCACGTAAGGCTTTGTTATCAGGTTGCTCTGCTGGGGGTTTAGCAGCATTTTTACATTGTGACGACTTCACTAAGTATGTACCAAGTAATGCCAGTGTAAAATGCTTGAGTGATGCTGGATTCTTTCTTGATGAAAGAGACATAGGTTTGAATCACACAATGAGGTCTTTCTATAAAGACCTTGTCTCTCTACAGGGTGTAGAAAAGAATCTGGATAAGAACTGCACCGGATCCCTTCCTTTTCCTGAGTTGTGTATTTTCCCACAGTATGCATTGAGATTCATCACACCGCCTTTTTTCATCTTGAACTCGGCTTATGATGTTTACCAGTTCCATCATGGATTGGTGCCATCTTCAGCTGATCCACGCGGACACTGGAACCGTTGCAAACTGGATCCGGCAGCATGCAATCCTGCTCAGTTAGATGTATTGCAAGGTTTCAGGCGCGACATGCTTGTTGCTTTGGCATTGTTCTACAAGTACTCGGCAAGAGGGGGGATGTACATCAACTCATGCTTTGCTCACTGCCAAAGTGAGTCCCAGGACACATGGTTTGGGGTTGATTCTCCAAGAATACACAACAAGACCATTGCAGAAGCTGTAGGTGACTGGTATTTTAGCAGAAGGATAACTAATAAGGAAGTTGACTGTGCATACCCTTGTGACACTACCTGCCATAACCTTATACCAGCAAACACAAGCTCAGGTCTTGTAGAAGATCTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

420

Amino Acids

46.75

Weight (kDa)

8.05

Isoelectric Point (pI)

45.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 36 - 404 6e-161 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1214
AccB1I GGYRCC 2 cut(s) 212, 880
AccB7I CCANNNNNTGG 1 cut(s) 118
AccBSI CCGCTC 1 cut(s) 152
AccII CGCG 3 cut(s) 80, 903, 981
AciI CCGC 5 cut(s) 44, 107, 150, 824, 903
AclI AACGTT 1 cut(s) 461
AclWI GGATC 5 cut(s) 759, 772, 890, 923, 936
AcuI CTGAAG 1 cut(s) 873
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 3 cut(s) 615, 1016, 1037
AfiI CCNNNNNNNGG 2 cut(s) 118, 155
AgsI TTSAA 2 cut(s) 685, 841
AjnI CCWGG 1 cut(s) 1074
AluBI AGCT 5 cut(s) 115, 422, 893, 1133, 1236
AluI AGCT 5 cut(s) 115, 422, 893, 1133, 1236
Alw26I GTCTC 3 cut(s) 429, 666, 724
AlwI GGATC 5 cut(s) 759, 772, 890, 923, 936
AlwNI CAGNNNCTG 1 cut(s) 1133
ApeKI GCWGC 4 cut(s) 67, 103, 578, 935
ArsI GACNNNNNNTTYG 2 cut(s) 1070, 1102
Asp700I GAANNNNTTC 1 cut(s) 304
AspLEI GCGC 6 cut(s) 67, 80, 82, 161, 215, 981
AsuHPI GGTGA 2 cut(s) 133, 1151
BamHI GGATCC 2 cut(s) 764, 928
BanI GGYRCC 2 cut(s) 212, 880
BarI GAAGNNNNNNTAC 4 cut(s) 292, 324, 346, 378
BbsI GAAGAC 1 cut(s) 23
BbvI GCAGC 4 cut(s) 54, 115, 590, 947
BccI CCATC 5 cut(s) 167, 410, 506, 876, 892
BciT130I CCWGG 1 cut(s) 1076
BcoDI GTCTC 3 cut(s) 429, 666, 724
BfmI CTRYAG 2 cut(s) 725, 1134
BfoI RGCGCY 3 cut(s) 68, 162, 216
BfuAI ACCTGC 1 cut(s) 1214
BglII AGATCT 1 cut(s) 1252
BisI GCNGC 5 cut(s) 68, 104, 107, 579, 936
BlsI GCNGC 5 cut(s) 69, 105, 108, 580, 937
BmcAI AGTACT 1 cut(s) 1016
Bme1390I CCNGG 1 cut(s) 1076
BmiI GGNNCC 6 cut(s) 214, 392, 766, 882, 914, 930
BmrFI CCNGG 1 cut(s) 1076
BmsI GCATC 1 cut(s) 637
BoxI GACNNNNGTC 1 cut(s) 134
BpiI GAAGAC 1 cut(s) 23
Bpu10I CCTNAGC 1 cut(s) 1237
BpuEI CTTGAG 1 cut(s) 661
BsaAI YACGTR 1 cut(s) 539
BsaBI GATNNNNATC 1 cut(s) 1037
BsaHI GRCGYC 1 cut(s) 213
BsaJI CCNNGG 2 cut(s) 83, 1074
BsaWI WCCGGW 1 cut(s) 761
BsaXI ACNNNNNCTCC 2 cut(s) 426, 456
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 155
Bse1I ACTGG 6 cut(s) 233, 627, 862, 914, 930, 1148
Bse3DI GCAATG 1 cut(s) 1122
Bse8I GATNNNNATC 1 cut(s) 1037
BseBI CCWGG 1 cut(s) 1076
BseDI CCNNGG 2 cut(s) 83, 1074
BseGI GGATG 2 cut(s) 178, 1038
BseJI GATNNNNATC 1 cut(s) 1037
BseLI CCNNNNNNNGG 2 cut(s) 118, 155
BseMI GCAATG 1 cut(s) 1122
BseMII CTCAG 3 cut(s) 771, 965, 1251
BseNI ACTGG 6 cut(s) 233, 627, 862, 914, 930, 1148
BsePI GCGCGC 1 cut(s) 78
BseXI GCAGC 4 cut(s) 54, 115, 590, 947
BseYI CCCAGC 1 cut(s) 566
BsgI GTGCAG 1 cut(s) 742
Bsh1236I CGCG 3 cut(s) 80, 903, 981
BshNI GGYRCC 2 cut(s) 212, 880
BsiSI CCGG 4 cut(s) 216, 432, 762, 932
BslFI GGGAC 2 cut(s) 469, 1057
BslI CCNNNNNNNGG 2 cut(s) 118, 155
BsmAI GTCTC 3 cut(s) 429, 666, 724
BsmBI CGTCTC 1 cut(s) 429
BsmFI GGGAC 2 cut(s) 469, 1057
BsmI GAATGC 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 1035
Bsp143I GATC 5 cut(s) 508, 764, 895, 928, 1252
BspACI CCGC 5 cut(s) 44, 107, 150, 824, 903
BspCNI CTCAG 3 cut(s) 772, 964, 1250
BspFNI CGCG 3 cut(s) 80, 903, 981
BspLI GGNNCC 6 cut(s) 214, 392, 766, 882, 914, 930
BspMI ACCTGC 1 cut(s) 1214
BspPI GGATC 5 cut(s) 759, 772, 890, 923, 936
BspQI GCTCTTC 1 cut(s) 26
BspT107I GGYRCC 2 cut(s) 212, 880
BsrBI CCGCTC 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 1122
BsrGI TGTACA 1 cut(s) 1035
BsrI ACTGG 6 cut(s) 233, 627, 862, 914, 930, 1148
BssECI CCNNGG 2 cut(s) 83, 1074
BssHII GCGCGC 1 cut(s) 78
BssMI GATC 5 cut(s) 508, 764, 895, 928, 1252
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 1 cut(s) 83
Bst2UI CCWGG 1 cut(s) 1076
Bst4CI ACNGT 6 cut(s) 136, 240, 395, 801, 917, 1184
Bst6I CTCTTC 2 cut(s) 26, 295
BstACI GRCGYC 1 cut(s) 213
BstAUI TGTACA 1 cut(s) 1035
BstBAI YACGTR 1 cut(s) 539
BstC8I GCNNGC 3 cut(s) 80, 186, 940
BstDEI CTNAG 5 cut(s) 350, 606, 780, 951, 1237
BstF5I GGATG 2 cut(s) 178, 1038
BstFNI CGCG 3 cut(s) 80, 903, 981
BstH2I RGCGCY 3 cut(s) 68, 162, 216
BstHHI GCGC 6 cut(s) 67, 80, 82, 161, 215, 981
BstKTI GATC 5 cut(s) 511, 767, 898, 931, 1255
BstMAI GTCTC 3 cut(s) 429, 666, 724
BstMBI GATC 5 cut(s) 508, 764, 895, 928, 1252
BstMWI GCNNNNNNNGC 8 cut(s) 50, 112, 158, 165, 428, 437, 542, 985
BstNI CCWGG 1 cut(s) 1076
BstNSI RCATGY 2 cut(s) 942, 988
BstPAI GACNNNNGTC 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 1074
BstSFI CTRYAG 2 cut(s) 725, 1134
BstUI CGCG 3 cut(s) 80, 903, 981
BstV1I GCAGC 4 cut(s) 54, 115, 590, 947
BstV2I GAAGAC 1 cut(s) 23
BstX2I RGATCY 3 cut(s) 764, 928, 1252
BstYI RGATCY 3 cut(s) 764, 928, 1252
BtsCI GGATG 2 cut(s) 178, 1038
BtsI GCAGTG 2 cut(s) 447, 1057
BtsIMutI CAGTG 4 cut(s) 447, 634, 907, 1057
BveI ACCTGC 1 cut(s) 1214
Cac8I GCNNGC 3 cut(s) 80, 186, 940
CaiI CAGNNNCTG 1 cut(s) 1133
CfoI GCGC 6 cut(s) 67, 80, 82, 161, 215, 981
CseI GACGC 1 cut(s) 446
Csp6I GTAC 3 cut(s) 614, 1015, 1036
CviAII CATG 6 cut(s) 127, 872, 939, 985, 1047, 1083
CviQI GTAC 3 cut(s) 614, 1015, 1036
DdeI CTNAG 5 cut(s) 350, 606, 780, 951, 1237
DinI GGCGCC 1 cut(s) 214
DpnI GATC 5 cut(s) 510, 766, 897, 930, 1254
DpnII GATC 5 cut(s) 508, 764, 895, 928, 1252
Eam1104I CTCTTC 2 cut(s) 26, 295
EarI CTCTTC 2 cut(s) 26, 295
Eco130I CCWWGG 1 cut(s) 83
Eco57I CTGAAG 1 cut(s) 873
EcoRII CCWGG 1 cut(s) 1074
EcoT14I CCWWGG 1 cut(s) 83
EcoT22I ATGCAT 1 cut(s) 808
EgeI GGCGCC 1 cut(s) 214
EheI GGCGCC 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 83
Esp3I CGTCTC 1 cut(s) 429
FaeI CATG 6 cut(s) 130, 875, 942, 988, 1050, 1086
FaqI GGGAC 2 cut(s) 469, 1057
FatI CATG 6 cut(s) 126, 871, 938, 984, 1046, 1082
FauI CCCGC 1 cut(s) 157
Fnu4HI GCNGC 5 cut(s) 68, 104, 107, 579, 936
FokI GGATG 2 cut(s) 185, 1045
Fsp4HI GCNGC 5 cut(s) 68, 104, 107, 579, 936
GlaI GCGC 6 cut(s) 66, 79, 81, 160, 214, 980
GluI GCNGC 5 cut(s) 68, 104, 107, 579, 936
GsaI CCCAGC 1 cut(s) 570
HaeII RGCGCY 3 cut(s) 68, 162, 216
HapII CCGG 4 cut(s) 216, 432, 762, 932
HgaI GACGC 1 cut(s) 446
HhaI GCGC 6 cut(s) 67, 80, 82, 161, 215, 981
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 6 cut(s) 130, 875, 942, 988, 1050, 1086
Hin6I GCGC 6 cut(s) 65, 78, 80, 159, 213, 979
HinP1I GCGC 6 cut(s) 65, 78, 80, 159, 213, 979
HincII GTYRAC 1 cut(s) 1180
HindII GTYRAC 1 cut(s) 1180
HinfI GANTC 9 cut(s) 272, 346, 403, 654, 685, 742, 813, 1070, 1097
HpaII CCGG 4 cut(s) 216, 432, 762, 932
HphI GGTGA 2 cut(s) 133, 1151
Hpy166II GTNNAC 3 cut(s) 124, 859, 1180
Hpy188I TCNGA 2 cut(s) 140, 476
Hpy188III TCNNGA 6 cut(s) 374, 506, 662, 746, 779, 838
Hpy8I GTNNAC 3 cut(s) 124, 859, 1180
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 2 cut(s) 779, 1152
HpyCH4III ACNGT 6 cut(s) 136, 240, 395, 801, 917, 1184
HpyCH4IV ACGT 4 cut(s) 59, 74, 461, 538
HpyCH4V TGCA 8 cut(s) 90, 759, 806, 921, 942, 967, 1127, 1187
HpyF10VI GCNNNNNNNGC 8 cut(s) 50, 112, 158, 165, 428, 437, 542, 985
HpyF3I CTNAG 5 cut(s) 350, 606, 780, 951, 1237
HpySE526I ACGT 4 cut(s) 59, 74, 461, 538
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 6 cut(s) 130, 875, 942, 988, 1050, 1086
HspAI GCGC 6 cut(s) 65, 78, 80, 159, 213, 979
KasI GGCGCC 1 cut(s) 212
Kzo9I GATC 5 cut(s) 508, 764, 895, 928, 1252
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 2 cut(s) 218, 419
Lsp1109I GCAGC 4 cut(s) 54, 115, 590, 947
LweI GCATC 1 cut(s) 637
MaeII ACGT 4 cut(s) 59, 74, 461, 538
MaeIII GTNAC 5 cut(s) 4, 55, 593, 1139, 1196
MalI GATC 5 cut(s) 510, 766, 897, 930, 1254
MbiI CCGCTC 1 cut(s) 152
MboI GATC 5 cut(s) 508, 764, 895, 928, 1252
MboII GAAGA 7 cut(s) 28, 43, 312, 412, 503, 879, 1262
MfeI CAATTG 1 cut(s) 385
MflI RGATCY 3 cut(s) 764, 928, 1252
MluCI AATT 2 cut(s) 385, 1258
Mly113I GGCGCC 1 cut(s) 213
MlyI GAGTC 2 cut(s) 266, 1079
MnlI CCTC 9 cut(s) 197, 238, 245, 279, 296, 325, 470, 690, 1019
Mph1103I ATGCAT 1 cut(s) 808
MroXI GAANNNNTTC 1 cut(s) 304
MseI TTAA 2 cut(s) 369, 1261
MspA1I CMGCKG 1 cut(s) 893
MspI CCGG 4 cut(s) 216, 432, 762, 932
MspR9I CCNGG 1 cut(s) 1076
MunI CAATTG 1 cut(s) 385
Mva1269I GAATGC 1 cut(s) 46
MvaI CCWGG 1 cut(s) 1076
MvnI CGCG 3 cut(s) 80, 903, 981
MwoI GCNNNNNNNGC 8 cut(s) 50, 112, 158, 165, 428, 437, 542, 985
NarI GGCGCC 1 cut(s) 213
NdeII GATC 5 cut(s) 508, 764, 895, 928, 1252
NlaIII CATG 6 cut(s) 130, 875, 942, 988, 1050, 1086
NlaIV GGNNCC 6 cut(s) 214, 392, 766, 882, 914, 930
NmeAIII GCCGAG 3 cut(s) 135, 824, 998
NmuCI GTSAC 4 cut(s) 55, 593, 1139, 1196
NsiI ATGCAT 1 cut(s) 808
NspI RCATGY 2 cut(s) 942, 988
PaeI GCATGC 1 cut(s) 942
PauI GCGCGC 1 cut(s) 78
PciSI GCTCTTC 1 cut(s) 26
PcsI WCGNNNNNNNCGW 1 cut(s) 26
PctI GAATGC 1 cut(s) 46
PdmI GAANNNNTTC 1 cut(s) 304
PfeI GAWTC 7 cut(s) 346, 403, 654, 685, 742, 813, 1097
PflFI GACNNNGTC 1 cut(s) 716
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 5 cut(s) 69, 105, 108, 580, 937
PleI GAGTC 2 cut(s) 266, 1078
PluTI GGCGCC 1 cut(s) 216
PpsI GAGTC 2 cut(s) 266, 1078
Ppu21I YACGTR 1 cut(s) 539
PshAI GACNNNNGTC 1 cut(s) 134
Psp1406I AACGTT 1 cut(s) 461
Psp6I CCWGG 1 cut(s) 1074
PspFI CCCAGC 1 cut(s) 566
PspGI CCWGG 1 cut(s) 1074
PspN4I GGNNCC 6 cut(s) 214, 392, 766, 882, 914, 930
PstNI CAGNNNCTG 1 cut(s) 1133
PsuI RGATCY 3 cut(s) 764, 928, 1252
PsyI GACNNNGTC 1 cut(s) 716
PteI GCGCGC 1 cut(s) 78
PvuII CAGCTG 1 cut(s) 893
RsaI GTAC 3 cut(s) 615, 1016, 1037
RsaNI GTAC 3 cut(s) 614, 1015, 1036
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 2 cut(s) 369, 1261
SatI GCNGC 5 cut(s) 68, 104, 107, 579, 936
Sau3AI GATC 5 cut(s) 508, 764, 895, 928, 1252
ScaI AGTACT 1 cut(s) 1016
SchI GAGTC 2 cut(s) 266, 1079
ScrFI CCNGG 1 cut(s) 1076
SfaNI GCATC 1 cut(s) 637
SfcI CTRYAG 2 cut(s) 725, 1134
SfoI GGCGCC 1 cut(s) 214
SmlI CTYRAG 1 cut(s) 640
SmoI CTYRAG 1 cut(s) 640
SphI GCATGC 1 cut(s) 942
Sse9I AATT 2 cut(s) 385, 1258
SsiI CCGC 5 cut(s) 44, 107, 150, 824, 903
SspDI GGCGCC 1 cut(s) 212
StyD4I CCNGG 1 cut(s) 1074
StyI CCWWGG 1 cut(s) 83
TaaI ACNGT 6 cut(s) 136, 240, 395, 801, 917, 1184
TaiI ACGT 4 cut(s) 62, 77, 464, 541
TaqI TCGA 1 cut(s) 406
TasI AATT 2 cut(s) 385, 1258
TatI WGTACW 2 cut(s) 1014, 1035
TauI GCSGC 1 cut(s) 109
TfiI GAWTC 7 cut(s) 346, 403, 654, 685, 742, 813, 1097
Tru1I TTAA 2 cut(s) 369, 1261
Tru9I TTAA 2 cut(s) 369, 1261
TscAI CASTG 4 cut(s) 447, 634, 914, 1064
TseFI GTSAC 4 cut(s) 55, 593, 1139, 1196
TseI GCWGC 4 cut(s) 67, 103, 578, 935
Tsp45I GTSAC 4 cut(s) 55, 593, 1139, 1196
TspDTI ATGAA 5 cut(s) 29, 414, 681, 805, 823
TspRI CASTG 4 cut(s) 447, 634, 914, 1064
Tth111I GACNNNGTC 1 cut(s) 716
Van91I CCANNNNNTGG 1 cut(s) 118
XceI RCATGY 2 cut(s) 942, 988
XcmI CCANNNNNNNNNTGG 2 cut(s) 869, 906
XmnI GAANNNNTTC 1 cut(s) 304
ZrmI AGTACT 1 cut(s) 1016
Zsp2I ATGCAT 1 cut(s) 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.