RchiOBHm_Chr1g0336351

pentatricopeptide repeat-containing protein At5g04810

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0011908
Physical Location & Seq
Reverse (-)
28020237 .. 28028633
8397 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56431

Sequence Viewer

Length: 2817 bp
ATGGAGTTTTCAGTGTCCACCCCACGCTATTACTATCCCAATCCCAAACCCACTACTACCACCACCACCTTTTCTCTCAAACCCCCAAATTCCCAAGCCTCCTCCTCCCCTCTCTCCTCATCTAACCCACTTCGTCGCCCCAAAACCATCACCACTCCTCCTCCTCCTCAGCCCACCAAACCCATCTCCAAAATCCCCTCTAACCCCTTCAAGAACCTCACTAATCCCACCCATGTCCCCACTCCACCGATTATTACCACCACCGATTCTAACACCTCTTCTCTCACCAACAAGCTCTGGCTCACCAGCAAGCTCTCTCCTCCTCCACCGCCACCAGACATAGAAGACGACGACGAAGAAGAAGCAGTCAAGTACAATGAACCCATAAAGAAATCGCAGCTACCCAAAGTCGAGTTCCGGCAAGAGGGTAAGATTTTCGTGGGGAATTTGCCCAACTGGCTCAAGAAAAACGAGGTCTCCGAGTTTTTCCGGCAATTCGGACCGATAACGAATGTGATTCTGATCAAGGGCCACGACAGCACTGAGAGAAATGCTGGTTTCGGATTCGTTATATACGGCGGCCCCAATGCGACCAAGTCTGCCATGAAGGCGGTGGAGTTTGACGGCGTGGAGTTTCACGGGAGGGTTCTGACTGTTAAGTTGGACGATGGGGGCAGATTGAAGGACAAAGCTGAGCAGAGGACGCGGTGGATTGAGCAAGACTCCGGCGACGGTGTTGAGTACCGAGCTCGGTCTAAGTGGCACAAGGAGAGGCAGAGCTCTCGCAATGCCTTGCGCAAGGTTATGGAGACGGAGCCGGAGAACTGGCAGGCTGTGGTTCAGACTTTTGAGAGGATTAACAAGCCTTCTAGAGGAGAGTATGGTCTGATGGTCAAGTATTATGCTAGGCGAGGGGACATGCATCGTGCTCGTGAAACATTTGAGAGCATGAGAGCAAGAGGAATAGAGCCGACTTCACATGTATACACAAGCCTGATTCACGCTTATGCAGTTGGCAGAGACATGGAAGAAGCATTATCTTGTGTTAGAAAAATGAAGGATGAGGGCATTGAAATGAGTTTGGTGACTTACAGTATTATTGTTGGGGGATTTGCAAAAGTTGGCAATGCCGAAGCTGCAGATCACTGGTTTAAGGAGGCCAAAGAGAGACATACAACTTTAAATGCAATCATTTATGGGAATATTGTTTATGCTCATTGTCAATCATGTAATATGGATCGAGCTGAGGCATTGGTACGAGAAATGGAAGAAGCAGGTATAGATGCTCCTATCGACATATATCACACCATGATGGATGGTTACACAATGGTTGGCAATGAAGATAAATGTTTGATTGTGTTTGAGCGACTGAAGGAATGCGGCTTTACACCTTCTGTCATCAGTTATGGATGTCTGATCAATCTTTACATCAAGGTTGGCAAAGTTTCTAAGGCATTGGAAGTCAGCAAAATGATGGAAACAGCTAGCATAAAACATAACATGAAGACGTACTCCATGTTGATCAATGGATTCTTGAAGTTAAAAGACTGGGCTAATGCATTTGCAGTTATAGAAGATTTGATGAGGGATGGTTTGAAGCCCGATGTAGTCCTGTATAATAACATTATAACAGCATTTTGTGGAATGGGTAACATGAATCGCGCTGTTCGCACCGTCAAAGAAATGCAGAGGGAGAGGCATAGACCTACATCGCGTACGTTTATGCCGATAATACATGGTTTTGCAAGAGCTGGAGATATGAGAAGAGCCCTAGAGATTTTTGACATGATGCGGATGAGTGGATGCATTCCAACTGTCCATACTTTCAATGCTCTAGTTCTTGGTCTTGTGGAGAAGCGGCAGATGGAGAAGGCTGTTGAAATATTGGATGAGATGACACTTGCAGGAATTAGTCCAAATGAACACACATACACAACTATCATGAATGGTTATGCATCTTTAGGTGATACTGGGAAAGCTTTTGAGTATTTTTCCAAATTGAGGAATGATGGCCTAGAGATTGATGTTTTCACATATGAGGCATTGCTGAAGGCATGTTGCAAAGCAGGCAAAATGCAGAGTGCTTTAGCTGTCACCAAGGAAATGAGTGCTCAAAAAATTCCAAGGAACACCTTCGTGTACAACATATTAGTTGATGGATGGGCTCGAAGAGGTGATGTTTGGGAGGCTGCTGACTTGATGCAGCAAATGAAACGGGAAGGGGTTCAACCTGATATCCACTCCTACACGTCTTTCATAAATGCTTGTTGCAAGGCCGGAGACATGCAGAGAGCTGCAAAAACAATTGAAGAAATGAAAACTTTTGGAGTGAAGCCAAATGTTAAAACTTATACCACATTGATACATGGTTGGGCGCGTGCTTCTCTACCGGAGAAGGCTTTGAGATGCTTCAAAGATATGAAGTTGGCTGGATTGAAGCCAGACAAAGCTGTGTACCATTGCTTGATGACGTCACTGTTGTCAAGGGCTACTGTTGCTGAAGCTTACATTTATTCTGGTCTTCTGTCTATCTGCAAGGAGATGATAGAATCTGGATTGACTGTAGATATGGGAACTGCAGTTCACTGGTCGAGGTGCTTACGCAAAATTGAAAGAACAGGTGGTGAGCTTACCGAAGCCCTGCAGAAGACCTTCCCTCCTGATTGGAACTCTTGTCATACTTTGTACGTGAGTTCCGACGTAGACAGTGATGATGAAGTTGACATTACTAGTGATGGTGATGATGATGACATGGAGTTTGCTAGTAGAATAGATAACGTTATGATGAATGATGAGGATAGTCTAAAGCACGGATAA

Protein Analysis

938

Amino Acids

105.7

Weight (kDa)

7.28

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 145 - 217 2e-11 RNA recognition motif
PPR_2 PF13041 296 - 337 2.6e-08 PPR repeat family
TPR_24 PF23276 302 - 415 1e-07 Fungal tetratrico peptide repeats
PPR_long PF17177 307 - 380 3.1e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 320 - 371 6.8e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 328 - 373 1.3e-09 PPR repeat family
PPR PF01535 328 - 357 6e-06 PPR repeat
PPR_3 PF13812 418 - 476 2.6e-08 Pentatricopeptide repeat domain
TPR_24 PF23276 453 - 561 7.2e-09 Fungal tetratrico peptide repeats
PPR_2 PF13041 464 - 513 4.2e-10 PPR repeat family
PPR_long PF17177 518 - 612 3.2e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 523 - 578 4.5e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 530 - 563 9e-08 PPR repeat
PPR_2 PF13041 534 - 583 5.7e-11 PPR repeat family
TPR_24 PF23276 571 - 666 2.8e-09 Fungal tetratrico peptide repeats
PPR_2 PF13041 573 - 616 1.8e-08 PPR repeat family
PPR_3 PF13812 573 - 613 5.4e-08 Pentatricopeptide repeat domain
PPR_long PF17177 588 - 694 1.3e-11 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 604 - 652 8.3e-14 PPR repeat family
PPR_2 PF13041 643 - 688 2e-10 PPR repeat family
PPR_3 PF13812 665 - 718 7.2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 670 - 703 9.9e-07 PPR repeat
PPR_2 PF13041 675 - 722 1.3e-09 PPR repeat family
PPR_long PF17177 679 - 824 8.2e-15 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 686 - 803 6.2e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 710 - 758 2.1e-14 PPR repeat family
TPR_24 PF23276 722 - 825 3.9e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 733 - 784 2.4e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 741 - 773 1.6e-07 PPR repeat
PPR_2 PF13041 747 - 792 1.1e-13 PPR repeat family
PPR PF01535 748 - 777 2.8e-06 PPR repeat
PPR_3 PF13812 771 - 823 1.5e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 776 - 808 2.1e-06 PPR repeat
PPR_2 PF13041 783 - 826 4.2e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011908)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04810 AT5G04810
fragaria_vesca FvH4_7g06690
malus_domestica MD07G1061700.v1.1
prunus_persica Prupe.2G071400_v2.0.a1 Prupe.2G071400_v2.0.a1 Prupe.2G071400_v2.0.a1
pyrus_communis pycom07g04720
rosa_chinensis RchiOBHm_Chr1g0336351
rosa_laevigata RLG00000029372
rosa_multiflora Rmu_sc0005261.1_g000013 Rmu_sc0005480.1_g000007
rosa_roxburghii Rroxscaffold_4G00315730
rosa_rugosa Rorug01G0125300
rosa_samantha Rh1AG144500 Rh1BG114100 Rh1CG136000 Rh1DG151200
rosa_wichuraiana Rw1G011840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1628
AatII GACGTC 1 cut(s) 2474
Acc16I TGCGCA 1 cut(s) 797
Acc36I ACCTGC 1 cut(s) 1265
AccI GTMKAC 2 cut(s) 984, 2703
AccII CGCG 4 cut(s) 706, 1662, 1714, 2377
AciI CCGC 7 cut(s) 329, 579, 611, 706, 1380, 1792, 1858
AclI AACGTT 1 cut(s) 2778
AclWI GGATC 1 cut(s) 1245
AcsI RAATTY 3 cut(s) 88, 445, 2118
AcuI CTGAAG 3 cut(s) 1391, 2069, 2520
AcyI GRCGYC 1 cut(s) 2471
AfaI GTAC 8 cut(s) 374, 743, 1257, 1511, 1717, 2141, 2456, 2687
AfiI CCNNNNNNNGG 3 cut(s) 424, 872, 2001
AflIII ACRYGT 2 cut(s) 979, 2247
AhlI ACTAGT 1 cut(s) 2729
AjiI CACGTC 1 cut(s) 2250
AjuI GAANNNNNNNTTGG 2 cut(s) 398, 430
AleI CACNNNNGTG 1 cut(s) 2135
AloI GAACNNNNNNTCC 2 cut(s) 2565, 2597
Alw21I GWGCWC 4 cut(s) 751, 782, 931, 2113
Alw26I GTCTC 5 cut(s) 481, 803, 1014, 1162, 2274
AlwI GGATC 1 cut(s) 1245
AoxI GGCC 5 cut(s) 529, 580, 1158, 2011, 2274
ApeKI GCWGC 5 cut(s) 397, 1136, 2189, 2203, 2294
ApoI RAATTY 3 cut(s) 88, 445, 2118
ArsI GACNNNNNNTTYG 2 cut(s) 2741, 2773
Asp700I GAANNNNTTC 3 cut(s) 2132, 2223, 2651
AspLEI GCGC 3 cut(s) 798, 1664, 2377
AspS9I GGNCC 3 cut(s) 500, 529, 581
AsuHPI GGTGA 9 cut(s) 142, 277, 295, 1096, 1976, 2086, 2186, 2636, 2750
AsuNHI GCTAGC 1 cut(s) 1484
AvaII GGWCC 1 cut(s) 500
BanII GRGCYC 4 cut(s) 751, 782, 1771, 2167
BauI CACGAG 1 cut(s) 930
BbsI GAAGAC 4 cut(s) 351, 1511, 2513, 2654
Bbv12I GWGCWC 4 cut(s) 751, 782, 931, 2113
BbvCI CCTCAGC 2 cut(s) 168, 1245
BbvI GCAGC 5 cut(s) 409, 1123, 2176, 2215, 2281
BceAI ACGGC 2 cut(s) 592, 640
BcgI CGANNNNNNTGC 4 cut(s) 764, 798, 911, 945
BclI TGATCA 3 cut(s) 522, 1416, 1521
BcoDI GTCTC 5 cut(s) 481, 803, 1014, 1162, 2274
BcuI ACTAGT 1 cut(s) 2729
BfaI CTAG 8 cut(s) 870, 906, 1485, 1772, 1835, 2015, 2730, 2763
BfmI CTRYAG 4 cut(s) 1137, 2562, 2577, 2642
BfuAI ACCTGC 1 cut(s) 1265
BglI GCCNNNNNGGC 2 cut(s) 457, 608
BisI GCNGC 8 cut(s) 398, 580, 1137, 1381, 1859, 2190, 2204, 2295
BlpI GCTNAGC 1 cut(s) 693
BlsI GCNGC 8 cut(s) 399, 581, 1138, 1382, 1860, 2191, 2205, 2296
Bme18I GGWCC 1 cut(s) 500
BmgBI CACGTC 1 cut(s) 2250
BmgT120I GGNCC 3 cut(s) 500, 529, 581
BmiI GGNNCC 2 cut(s) 583, 816
BmrI ACTGGG 2 cut(s) 1558, 1980
BmsI GCATC 7 cut(s) 931, 1273, 1779, 1793, 1964, 2190, 2396
BmtI GCTAGC 1 cut(s) 1488
BmuI ACTGGG 2 cut(s) 1558, 1980
BpiI GAAGAC 4 cut(s) 351, 1511, 2513, 2654
BplI GAGNNNNNCTC 2 cut(s) 707, 739
BpmI CTGGAG 1 cut(s) 1773
Bpu10I CCTNAGC 2 cut(s) 168, 1245
Bpu1102I GCTNAGC 1 cut(s) 693
BpuEI CTTGAG 1 cut(s) 446
BsaAI YACGTR 1 cut(s) 2689
BsaBI GATNNNNATC 1 cut(s) 521
BsaHI GRCGYC 1 cut(s) 2471
BsaI GGTCTC 1 cut(s) 481
BsaJI CCNNGG 2 cut(s) 2097, 2123
BsaWI WCCGGW 1 cut(s) 2389
BsaXI ACNNNNNCTCC 8 cut(s) 142, 145, 172, 175, 461, 491, 2641, 2671
Bsc4I CCNNNNNNNGG 3 cut(s) 424, 872, 2001
Bse1I ACTGG 6 cut(s) 461, 830, 1151, 1553, 1975, 2591
Bse3DI GCAATG 5 cut(s) 793, 1132, 1342, 2042, 2458
Bse8I GATNNNNATC 1 cut(s) 521
BseDI CCNNGG 2 cut(s) 2097, 2123
BseGI GGATG 8 cut(s) 1066, 1321, 1415, 1594, 1800, 1808, 1894, 2165
BseJI GATNNNNATC 1 cut(s) 521
BseLI CCNNNNNNNGG 3 cut(s) 424, 872, 2001
BseMI GCAATG 5 cut(s) 793, 1132, 1342, 2042, 2458
BseMII CTCAG 4 cut(s) 182, 534, 684, 1236
BseNI ACTGG 6 cut(s) 461, 830, 1151, 1553, 1975, 2591
BseXI GCAGC 5 cut(s) 409, 1123, 2176, 2215, 2281
Bsh1236I CGCG 4 cut(s) 706, 1662, 1714, 2377
BshFI GGCC 5 cut(s) 531, 582, 1160, 2013, 2276
BsiHKAI GWGCWC 4 cut(s) 751, 782, 931, 2113
BsiSI CCGG 6 cut(s) 418, 490, 726, 818, 2277, 2390
BsiWI CGTACG 1 cut(s) 1715
BslFI GGGAC 2 cut(s) 221, 929
BslI CCNNNNNNNGG 3 cut(s) 424, 872, 2001
BsmAI GTCTC 5 cut(s) 481, 803, 1014, 1162, 2274
BsmBI CGTCTC 1 cut(s) 803
BsmFI GGGAC 2 cut(s) 221, 929
BsmI GAATGC 2 cut(s) 1382, 1806
BsnI GGCC 5 cut(s) 531, 582, 1160, 2013, 2276
Bso31I GGTCTC 1 cut(s) 481
Bsp1286I GDGCHC 6 cut(s) 751, 782, 931, 1771, 2113, 2167
Bsp1407I TGTACA 1 cut(s) 2139
Bsp143I GATC 5 cut(s) 522, 1141, 1237, 1416, 1521
Bsp1720I GCTNAGC 1 cut(s) 693
BspACI CCGC 7 cut(s) 329, 579, 611, 706, 1380, 1792, 1858
BspANI GGCC 5 cut(s) 531, 582, 1160, 2013, 2276
BspCNI CTCAG 4 cut(s) 181, 535, 685, 1237
BspFNI CGCG 4 cut(s) 706, 1662, 1714, 2377
BspHI TCATGA 1 cut(s) 1941
BspLI GGNNCC 2 cut(s) 583, 816
BspMAI CTGCAG 3 cut(s) 1141, 2581, 2646
BspMI ACCTGC 1 cut(s) 1265
BspOI GCTAGC 1 cut(s) 1488
BspPI GGATC 1 cut(s) 1245
BspQI GCTCTTC 1 cut(s) 1759
BspTNI GGTCTC 1 cut(s) 481
BsrDI GCAATG 5 cut(s) 793, 1132, 1342, 2042, 2458
BsrGI TGTACA 1 cut(s) 2139
BsrI ACTGG 6 cut(s) 461, 830, 1151, 1553, 1975, 2591
BssECI CCNNGG 2 cut(s) 2097, 2123
BssMI GATC 5 cut(s) 522, 1141, 1237, 1416, 1521
BssNAI GTATAC 1 cut(s) 985
BssNI GRCGYC 1 cut(s) 2471
BssSI CACGAG 1 cut(s) 930
BssT1I CCWWGG 2 cut(s) 2097, 2123
Bst1107I GTATAC 1 cut(s) 985
Bst2BI CACGAG 1 cut(s) 930
Bst4CI ACNGT 9 cut(s) 655, 734, 1094, 1675, 1816, 2478, 2494, 2563, 2708
Bst6I CTCTTC 3 cut(s) 283, 1759, 2164
BstACI GRCGYC 1 cut(s) 2471
BstAUI TGTACA 1 cut(s) 2139
BstBAI YACGTR 1 cut(s) 2689
BstC8I GCNNGC 5 cut(s) 311, 831, 1486, 2068, 2379
BstDEI CTNAG 6 cut(s) 168, 543, 693, 756, 1245, 1449
BstENI CCTNNNNNAGG 1 cut(s) 870
BstF5I GGATG 8 cut(s) 1066, 1321, 1415, 1594, 1800, 1808, 1894, 2165
BstFNI CGCG 4 cut(s) 706, 1662, 1714, 2377
BstHHI GCGC 3 cut(s) 798, 1664, 2377
BstKTI GATC 5 cut(s) 525, 1144, 1240, 1419, 1524
BstMAI GTCTC 5 cut(s) 481, 803, 1014, 1162, 2274
BstMBI GATC 5 cut(s) 522, 1141, 1237, 1416, 1521
BstMWI GCNNNNNNNGC 8 cut(s) 457, 537, 608, 703, 1136, 1668, 2067, 2495
BstNSI RCATGY 4 cut(s) 922, 983, 2058, 2287
BstSFI CTRYAG 4 cut(s) 1137, 2562, 2577, 2642
BstUI CGCG 4 cut(s) 706, 1662, 1714, 2377
BstV1I GCAGC 5 cut(s) 409, 1123, 2176, 2215, 2281
BstV2I GAAGAC 4 cut(s) 351, 1511, 2513, 2654
BstZ17I GTATAC 1 cut(s) 985
BsuRI GGCC 5 cut(s) 531, 582, 1160, 2013, 2276
BtgZI GCGATG 1 cut(s) 1695
BtrI CACGTC 1 cut(s) 2250
BtsCI GGATG 8 cut(s) 1066, 1321, 1415, 1594, 1800, 1808, 1894, 2165
BtsIMutI CAGTG 6 cut(s) 18, 540, 1144, 2474, 2584, 2713
BveI ACCTGC 1 cut(s) 1265
Cac8I GCNNGC 5 cut(s) 311, 831, 1486, 2068, 2379
CciI TCATGA 1 cut(s) 1941
CfoI GCGC 3 cut(s) 798, 1664, 2377
Cfr13I GGNCC 3 cut(s) 500, 529, 581
CpoI CGGWCCG 1 cut(s) 500
CseI GACGC 1 cut(s) 712
Csp6I GTAC 8 cut(s) 373, 742, 1256, 1510, 1716, 2140, 2455, 2686
CspI CGGWCCG 1 cut(s) 500
CviQI GTAC 8 cut(s) 373, 742, 1256, 1510, 1716, 2140, 2455, 2686
DdeI CTNAG 6 cut(s) 168, 543, 693, 756, 1245, 1449
DpnI GATC 5 cut(s) 524, 1143, 1239, 1418, 1523
DpnII GATC 5 cut(s) 522, 1141, 1237, 1416, 1521
DraI TTTAAA 1 cut(s) 1182
Eam1104I CTCTTC 3 cut(s) 283, 1759, 2164
EarI CTCTTC 3 cut(s) 283, 1759, 2164
Ecl136II GAGCTC 2 cut(s) 749, 780
Eco130I CCWWGG 2 cut(s) 2097, 2123
Eco24I GRGCYC 4 cut(s) 751, 782, 1771, 2167
Eco31I GGTCTC 1 cut(s) 481
Eco32I GATATC 1 cut(s) 2236
Eco47I GGWCC 1 cut(s) 500
Eco53kI GAGCTC 2 cut(s) 749, 780
Eco57I CTGAAG 3 cut(s) 1391, 2069, 2520
EcoICRI GAGCTC 2 cut(s) 749, 780
EcoNI CCTNNNNNAGG 1 cut(s) 870
EcoRV GATATC 1 cut(s) 2236
EcoT14I CCWWGG 2 cut(s) 2097, 2123
EcoT22I ATGCAT 4 cut(s) 924, 1561, 1808, 1957
EcoT38I GRGCYC 4 cut(s) 751, 782, 1771, 2167
ErhI CCWWGG 2 cut(s) 2097, 2123
Esp3I CGTCTC 1 cut(s) 803
FaqI GGGAC 2 cut(s) 221, 929
FauNDI CATATG 1 cut(s) 2035
FbaI TGATCA 3 cut(s) 522, 1416, 1521
FblI GTMKAC 2 cut(s) 984, 2703
Fnu4HI GCNGC 8 cut(s) 398, 580, 1137, 1381, 1859, 2190, 2204, 2295
FokI GGATG 8 cut(s) 1073, 1328, 1422, 1601, 1807, 1815, 1901, 2172
FriOI GRGCYC 4 cut(s) 751, 782, 1771, 2167
Fsp4HI GCNGC 8 cut(s) 398, 580, 1137, 1381, 1859, 2190, 2204, 2295
FspBI CTAG 8 cut(s) 870, 906, 1485, 1772, 1835, 2015, 2730, 2763
FspI TGCGCA 1 cut(s) 797
GlaI GCGC 3 cut(s) 797, 1663, 2376
GluI GCNGC 8 cut(s) 398, 580, 1137, 1381, 1859, 2190, 2204, 2295
GsuI CTGGAG 1 cut(s) 1773
HaeIII GGCC 5 cut(s) 531, 582, 1160, 2013, 2276
HapII CCGG 6 cut(s) 418, 490, 726, 818, 2277, 2390
HgaI GACGC 1 cut(s) 712
HhaI GCGC 3 cut(s) 798, 1664, 2377
Hin1I GRCGYC 1 cut(s) 2471
Hin6I GCGC 3 cut(s) 796, 1662, 2375
HinP1I GCGC 3 cut(s) 796, 1662, 2375
HincII GTYRAC 1 cut(s) 2722
HindII GTYRAC 1 cut(s) 2722
HindIII AAGCTT 2 cut(s) 1977, 2502
HinfI GANTC 8 cut(s) 266, 517, 564, 722, 997, 1530, 1657, 2549
HpaII CCGG 6 cut(s) 418, 490, 726, 818, 2277, 2390
HphI GGTGA 9 cut(s) 142, 277, 295, 1096, 1976, 2086, 2186, 2636, 2750
Hpy166II GTNNAC 7 cut(s) 18, 985, 2140, 2455, 2584, 2704, 2722
Hpy188I TCNGA 9 cut(s) 481, 500, 522, 563, 651, 843, 888, 1416, 2698
Hpy188III TCNNGA 8 cut(s) 211, 463, 870, 932, 1534, 1942, 2553, 2660
Hpy8I GTNNAC 7 cut(s) 18, 985, 2140, 2455, 2584, 2704, 2722
Hpy99I CGWCG 5 cut(s) 138, 353, 356, 734, 2702
HpyCH4III ACNGT 9 cut(s) 655, 734, 1094, 1675, 1816, 2478, 2494, 2563, 2708
HpyCH4IV ACGT 7 cut(s) 1508, 1718, 2249, 2471, 2688, 2700, 2778
HpyF10VI GCNNNNNNNGC 8 cut(s) 457, 537, 608, 703, 1136, 1668, 2067, 2495
HpyF3I CTNAG 6 cut(s) 168, 543, 693, 756, 1245, 1449
HpySE526I ACGT 7 cut(s) 1508, 1718, 2249, 2471, 2688, 2700, 2778
Hsp92I GRCGYC 1 cut(s) 2471
HspAI GCGC 3 cut(s) 796, 1662, 2375
Ksp22I TGATCA 3 cut(s) 522, 1416, 1521
Kzo9I GATC 5 cut(s) 522, 1141, 1237, 1416, 1521
LguI GCTCTTC 1 cut(s) 1759
LmnI GCTCC 2 cut(s) 814, 1291
Lsp1109I GCAGC 5 cut(s) 409, 1123, 2176, 2215, 2281
LweI GCATC 7 cut(s) 931, 1273, 1779, 1793, 1964, 2190, 2396
MaeI CTAG 8 cut(s) 870, 906, 1485, 1772, 1835, 2015, 2730, 2763
MaeII ACGT 7 cut(s) 1508, 1718, 2249, 2471, 2688, 2700, 2778
MaeIII GTNAC 5 cut(s) 1084, 1319, 1649, 2092, 2472
MalI GATC 5 cut(s) 524, 1143, 1239, 1418, 1523
MboI GATC 5 cut(s) 522, 1141, 1237, 1416, 1521
MfeI CAATTG 1 cut(s) 2304
MhlI GDGCHC 6 cut(s) 751, 782, 931, 1771, 2113, 2167
MluCI AATT 8 cut(s) 88, 445, 494, 1908, 1997, 2118, 2304, 2607
MlyI GAGTC 1 cut(s) 716
MmeI TCCRAC 3 cut(s) 642, 1835, 2721
Mph1103I ATGCAT 4 cut(s) 924, 1561, 1808, 1957
MroXI GAANNNNTTC 3 cut(s) 2132, 2223, 2651
MseI TTAA 6 cut(s) 657, 858, 1152, 1181, 1541, 2343
MslI CAYNNNNRTG 4 cut(s) 1005, 1073, 1310, 2135
MspI CCGG 6 cut(s) 418, 490, 726, 818, 2277, 2390
MunI CAATTG 1 cut(s) 2304
Mva1269I GAATGC 2 cut(s) 1382, 1806
MvnI CGCG 4 cut(s) 706, 1662, 1714, 2377
MwoI GCNNNNNNNGC 8 cut(s) 457, 537, 608, 703, 1136, 1668, 2067, 2495
NdeI CATATG 1 cut(s) 2035
NdeII GATC 5 cut(s) 522, 1141, 1237, 1416, 1521
NheI GCTAGC 1 cut(s) 1484
NlaIV GGNNCC 2 cut(s) 583, 816
NmuCI GTSAC 3 cut(s) 1084, 2092, 2472
NsbI TGCGCA 1 cut(s) 797
NsiI ATGCAT 4 cut(s) 924, 1561, 1808, 1957
NspI RCATGY 4 cut(s) 922, 983, 2058, 2287
OliI CACNNNNGTG 1 cut(s) 2135
PagI TCATGA 1 cut(s) 1941
PciI ACATGT 1 cut(s) 979
PciSI GCTCTTC 1 cut(s) 1759
PcsI WCGNNNNNNNCGW 3 cut(s) 477, 1724, 2694
PctI GAATGC 2 cut(s) 1382, 1806
PdmI GAANNNNTTC 3 cut(s) 2132, 2223, 2651
PfeI GAWTC 7 cut(s) 266, 517, 564, 997, 1530, 1657, 2549
Pfl23II CGTACG 1 cut(s) 1715
PflFI GACNNNGTC 1 cut(s) 595
PkrI GCNGC 8 cut(s) 399, 581, 1138, 1382, 1860, 2191, 2205, 2296
PleI GAGTC 1 cut(s) 716
PpsI GAGTC 1 cut(s) 716
Ppu21I YACGTR 1 cut(s) 2689
PscI ACATGT 1 cut(s) 979
PsiI TTATAA 1 cut(s) 1628
Psp124BI GAGCTC 2 cut(s) 751, 782
Psp1406I AACGTT 1 cut(s) 2778
PspLI CGTACG 1 cut(s) 1715
PspN4I GGNNCC 2 cut(s) 583, 816
PspPI GGNCC 3 cut(s) 500, 529, 581
PstI CTGCAG 3 cut(s) 1141, 2581, 2646
PsyI GACNNNGTC 1 cut(s) 595
RsaI GTAC 8 cut(s) 374, 743, 1257, 1511, 1717, 2141, 2456, 2687
RsaNI GTAC 8 cut(s) 373, 742, 1256, 1510, 1716, 2140, 2455, 2686
RseI CAYNNNNRTG 4 cut(s) 1005, 1073, 1310, 2135
Rsr2I CGGWCCG 1 cut(s) 500
RsrII CGGWCCG 1 cut(s) 500
SacI GAGCTC 2 cut(s) 751, 782
SapI GCTCTTC 1 cut(s) 1759
SaqAI TTAA 6 cut(s) 657, 858, 1152, 1181, 1541, 2343
SatI GCNGC 8 cut(s) 398, 580, 1137, 1381, 1859, 2190, 2204, 2295
Sau3AI GATC 5 cut(s) 522, 1141, 1237, 1416, 1521
Sau96I GGNCC 3 cut(s) 500, 529, 581
SchI GAGTC 1 cut(s) 716
SduI GDGCHC 6 cut(s) 751, 782, 931, 1771, 2113, 2167
SfaNI GCATC 7 cut(s) 931, 1273, 1779, 1793, 1964, 2190, 2396
SfcI CTRYAG 4 cut(s) 1137, 2562, 2577, 2642
SinI GGWCC 1 cut(s) 500
SmiMI CAYNNNNRTG 4 cut(s) 1005, 1073, 1310, 2135
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
SpeI ACTAGT 1 cut(s) 2729
Sse9I AATT 8 cut(s) 88, 445, 494, 1908, 1997, 2118, 2304, 2607
SsiI CCGC 7 cut(s) 329, 579, 611, 706, 1380, 1792, 1858
SspI AATATT 2 cut(s) 1204, 1884
SspMI CTAG 8 cut(s) 870, 906, 1485, 1772, 1835, 2015, 2730, 2763
SstI GAGCTC 2 cut(s) 751, 782
StyI CCWWGG 2 cut(s) 2097, 2123
TaaI ACNGT 9 cut(s) 655, 734, 1094, 1675, 1816, 2478, 2494, 2563, 2708
TaiI ACGT 7 cut(s) 1511, 1721, 2252, 2474, 2691, 2703, 2781
TaqI TCGA 5 cut(s) 411, 1240, 1293, 2167, 2591
TaqII GACCGA 2 cut(s) 517, 741
TasI AATT 8 cut(s) 88, 445, 494, 1908, 1997, 2118, 2304, 2607
TatI WGTACW 2 cut(s) 372, 2139
TauI GCSGC 3 cut(s) 582, 1383, 1861
TfiI GAWTC 7 cut(s) 266, 517, 564, 997, 1530, 1657, 2549
Tru1I TTAA 6 cut(s) 657, 858, 1152, 1181, 1541, 2343
Tru9I TTAA 6 cut(s) 657, 858, 1152, 1181, 1541, 2343
TscAI CASTG 6 cut(s) 18, 547, 1151, 2481, 2591, 2713
TseFI GTSAC 3 cut(s) 1084, 2092, 2472
TseI GCWGC 5 cut(s) 397, 1136, 2189, 2203, 2294
Tsp45I GTSAC 3 cut(s) 1084, 2092, 2472
TspGWI ACGGA 1 cut(s) 827
TspRI CASTG 6 cut(s) 18, 547, 1151, 2481, 2591, 2713
Tth111I GACNNNGTC 1 cut(s) 595
VpaK11BI GGWCC 1 cut(s) 500
XagI CCTNNNNNAGG 1 cut(s) 870
XapI RAATTY 3 cut(s) 88, 445, 2118
XbaI TCTAGA 1 cut(s) 869
XceI RCATGY 4 cut(s) 922, 983, 2058, 2287
XcmI CCANNNNNNNNNTGG 1 cut(s) 610
XmiI GTMKAC 2 cut(s) 984, 2703
XmnI GAANNNNTTC 3 cut(s) 2132, 2223, 2651
XspI CTAG 8 cut(s) 870, 906, 1485, 1772, 1835, 2015, 2730, 2763
ZraI GACGTC 1 cut(s) 2472
Zsp2I ATGCAT 4 cut(s) 924, 1561, 1808, 1957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.