Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0011908

Physical Location & Seq

Genomic Coordinates Forward (+)

40311624 .. 40319404

Gene Structure 7781 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00315730.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2838 bp

ATGGAGTTTTCAGTGTCCACCCCACGCTATTACTATCCAAATCCCAAACCCACTACTACCACCACCTCCTTTTCTCTCAAACCCCTTAATTCCCAAGCCTCCTCCTCCCCTCTCTCCTCATCTAACCAACTTCGTCGCCCCAAAACCATCACCACTCCTCCTCCTCAGCCCACCAAACCCATCTCCAAAATCCCCTCTAACCCCTTCAAGAATCTCACTAATCCCACCCATGTCCCCACTTCTCCGATTATTACCACCACCGATTCTAACACCTCTTCTCTCACCAACAAGCTCTGGCTCACCAGCAAGCTCTCTCCTCCTCCTCCTCCACCGCCACCAGACATAGAAGACGACGGCGGCGACGACGACGATGAAGAAGAGGCAGTCAAGTACAATGAACCCAAAAAGAAATCGCAGCTGCCTAAAGTCGAGTTCCGGCAAGAGGGTAAGGTTTTCGTGGGGAATTTACCCAACTGGCTCAACAAAAACGAGGTCTCGGAGTTTTTCCGGCAATTCGGACCGATAACGAATGTGATTCTGATCAAGGGCCACGACAGCACTGAGAGAAATGCTGGTTTCGGATTCGTTATATACGGCGGCCCCAATGCGACCAAGTCTGCCATGAAGGCGGTGGAGTTTGACGGCGTGGAGTTCCACGGGAGGGTTCTGACTGTTAAGTTGGACGATGGGGGCAGATTGAAGGACAAAGCTGAGCAGAGGACGCGGTGGATTGAGCAAGACTCCGGCGACGGTGTTGAGTACCGAGCTCGGTCTAAGTGGCACAAGGAGAGGCAGAGCTCTCGCAATGCCTTGCGCAAGGTTATGGAGACGGAGCCGGAGAACTGGCAGGGGGTGGTTCAGACTTTTGAGAGGATTAACAAGCCTTCTAGAGGAGAGTATGGTCTGATGGTCAAGTATTATGCTAGGCGAGGGGACATGCATCGTGCTCGCGAGACATTTGAGAGCATGAGAGCAAGAGGAATAGAGCCGACTTCACATGTATACACAAGCCTGATTCACGCTTATGCAGTTGGCAGAGACATGGAAGAAGCATTATCTTGTGTTAGAAAAATGAAGGACGAGGGCATTGAAATGAGTTTGGTGACTTACAGTATTATTGTTGGGGGATTTGCAAAAATTGGCAATGCCGAAGCTGCAGATCACTGGTTTAAGGAGGCCAAAGAGAGACATACAACTTTAAATGCAATCGTTTATGGGAATATTGTTTATGCTCATTGTCAAGCATGTAATATGGATCGAGCTGAGGCATTGGTACGGGAAATGGAAGAAGCAGGTATAGATGCTCCTATCGACATATATCACACCATGATGGATGGTTACACAATGGTTGGCAATGAAGATAAATGTTTGATTGTGTTTGAGCGACTGAAGGAATGTGGCTTTACACCTTCTGTCATCAGTTATGGATGTCTGATCAATCTTTACATCAAGGTTGGAAAAGTTTCTAAAGCATTGGAAGTCAGCAAAATGATGGAAACAGCCAGCATAAAACATAATATGAAGACGTACTCCATGTTGATCAATGGATTCTTGAAGTTAAAAGACTGGGCTAATGCATTTGCAGTTATAGAAGATTTGATGAAGGATGGTTTGAAGCCCGATGTAGTCCTGTATAATAACATTATAACAGCATTTTGTGGAATGGGTAACATGAATCGCGCTGTTCGCACCGTCAAAGAAATGCAGAGGGAGAGGCATAGACCTACATCACGTACGTTTATGCCGATAATACATGGTTTTGCAAGAGCTGGAGATATGAGAAGAGCCCTAGAGATTTTTGACATGATGAGGATGAGTGGATGCATTCCAACTGTCCATACTTTCAATGCTCTAGTTCTTGGTCTTGTGGAGAAGCGGCAGATGGAGAAGGCTGTTGAAATATTGGATGAGATGACACTTGCAGGCATCAGTCCAAATGAACACACATACACAACGATCATGAATGGTTATGCATCTTTAGGTGATACTGGGAAAGCTTTCGAGTATTTTTCCAAATTGAGGAATGATGGCCTAGAGATTGATGTTTTCACATATGAGGCATTGCTGAAGGCATGTTGCAAAGCAGGCAAAATGCAGAGTGCTTTGGCTGTCACCAAGGAAATGAGTGCTCAAAAAATTCCAAGGAACACCTTCGTGTTCAACATATTAGTTGATGGATGGGCTCGAAGAGGTGATGTTTGGGAGGCTGCTGACTTGATGCAGCAAATGAAACGGGAAGGGGTTCAACCTGATATCCACTCCTACACGTCTTTCATAAATGCTTGTTGCAAGGCCGGAGACATGCAGAGAGCCGCAAAAACAATTGAAGAAATGAAAACTTTTGGAGTGAAGCCAAATGTTAAAACTTATACCACACTGATACATGGTTGGGCGCGTGCTTCTCTACCGGAGAAGGCTTTGAGATGCTTCAAAGATATGAAGTTGGCTGGATTGAAGCCAGACAAAGCTGTGTACCATTGCTTGATGACATCACTGTTGTCAAGGGCTACTGTTGCTGAAGCTTACATTTATTCTGGCCTTCTGTCTATCTGCAAGGAGATGATAGAATCTGGATTGACTGTGGATATGGGAACTGCAGTTCACTGGTCGAGGTGCTTACGTAAAATTGAAAGAACAGGTGGTGAGCTTACAGAAGCCTTGCAGAAGACCTTCCCTCCTGATTGGAACTCTTGTCATACTTCGTATGTGAGTTCCGACGTAGACAGTGATGATGAAGTTGACATTATTAGTGATGGTGATGGTGATGATGACAGGGAGTTTGCTAGTAGAATAGATAACGTTATGATGACTGATGAGGATAGTCTGAAGCACGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3 RRM_1

Protein Analysis

945

Amino Acids

106.25

Weight (kDa)

6.9

Isoelectric Point (pI)

40.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_1	PF00076	151 - 223	9.9e-12	RNA recognition motif
PPR_2	PF13041	302 - 343	2.6e-08	PPR repeat family
TPR_24	PF23276	308 - 423	5.9e-08	Fungal tetratrico peptide repeats
PPR_long	PF17177	313 - 386	4e-07	Pentacotripeptide-repeat region of PRORP
PPR	PF01535	334 - 363	6.1e-06	PPR repeat
PPR_2	PF13041	335 - 379	2.7e-09	PPR repeat family
PPR_3	PF13812	335 - 377	1e-06	Pentatricopeptide repeat domain
PPR_3	PF13812	424 - 482	2.9e-08	Pentatricopeptide repeat domain
TPR_24	PF23276	459 - 567	4.7e-09	Fungal tetratrico peptide repeats
PPR_2	PF13041	470 - 519	4.3e-10	PPR repeat family
PPR_3	PF13812	529 - 584	1.4e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	536 - 569	9.1e-08	PPR repeat
PPR_2	PF13041	540 - 589	5.7e-11	PPR repeat family
TPR_24	PF23276	577 - 672	2.9e-09	Fungal tetratrico peptide repeats
PPR_2	PF13041	579 - 622	1.8e-08	PPR repeat family
PPR_3	PF13812	579 - 619	5.4e-08	Pentatricopeptide repeat domain
PPR_long	PF17177	594 - 700	1.3e-11	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	610 - 658	8.4e-14	PPR repeat family
PPR_2	PF13041	649 - 694	1.1e-10	PPR repeat family
PPR_3	PF13812	671 - 724	1.3e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	676 - 709	1e-06	PPR repeat
PPR_2	PF13041	681 - 728	4.5e-09	PPR repeat family
PPR_long	PF17177	687 - 830	1.7e-14	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	716 - 764	1e-13	PPR repeat family
PPR_3	PF13812	739 - 790	2.4e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	747 - 779	1.6e-07	PPR repeat
PPR_2	PF13041	750 - 798	4.1e-15	PPR repeat family
PPR	PF01535	754 - 783	2.8e-06	PPR repeat
PPR_3	PF13812	777 - 829	1.5e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	782 - 814	2.1e-06	PPR repeat
PPR_2	PF13041	789 - 832	4.3e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011908)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04810 AT5G04810
fragaria_vesca	FvH4_7g06690
malus_domestica	MD07G1061700.v1.1
prunus_persica	Prupe.2G071400_v2.0.a1 Prupe.2G071400_v2.0.a1 Prupe.2G071400_v2.0.a1
pyrus_communis	pycom07g04720
rosa_chinensis	RchiOBHm_Chr1g0336351
rosa_laevigata	RLG00000029372
rosa_multiflora	Rmu_sc0005261.1_g000013 Rmu_sc0005480.1_g000007
rosa_roxburghii	Rroxscaffold_4G00315730
rosa_rugosa	Rorug01G0125300
rosa_samantha	Rh1AG144500 Rh1BG114100 Rh1CG136000 Rh1DG151200
rosa_wichuraiana	Rw1G011840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1646
Acc16I	TGCGCA	1 cut(s)	815
Acc36I	ACCTGC	1 cut(s)	1283
AccI	GTMKAC	2 cut(s)	1002, 2721
AccII	CGCG	4 cut(s)	724, 951, 1680, 2395
AciI	CCGC	7 cut(s)	332, 357, 597, 629, 724, 1876, 2313
AclI	AACGTT	1 cut(s)	2799
AclWI	GGATC	1 cut(s)	1263
AcsI	RAATTY	2 cut(s)	463, 2136
AcuI	CTGAAG	3 cut(s)	1409, 2087, 2538
AfaI	GTAC	6 cut(s)	392, 761, 1275, 1529, 1735, 2474
AfiI	CCNNNNNNNGG	4 cut(s)	442, 661, 890, 2019
AflIII	ACRYGT	2 cut(s)	997, 2265
AjiI	CACGTC	1 cut(s)	2268
AleI	CACNNNNGTG	1 cut(s)	2153
AloI	GAACNNNNNNTCC	2 cut(s)	2583, 2615
Alw21I	GWGCWC	4 cut(s)	769, 800, 949, 2131
Alw26I	GTCTC	6 cut(s)	499, 821, 947, 1032, 1180, 2292
AlwI	GGATC	1 cut(s)	1263
AoxI	GGCC	6 cut(s)	547, 598, 1176, 2029, 2292, 2536
ApeKI	GCWGC	5 cut(s)	415, 418, 1154, 2207, 2221
ApoI	RAATTY	2 cut(s)	463, 2136
ArsI	GACNNNNNNTTYG	4 cut(s)	477, 509, 2762, 2794
Asp700I	GAANNNNTTC	5 cut(s)	1462, 1997, 2150, 2241, 2669
AspLEI	GCGC	3 cut(s)	816, 1682, 2395
AspS9I	GGNCC	3 cut(s)	518, 547, 599
AvaII	GGWCC	1 cut(s)	518
BanII	GRGCYC	4 cut(s)	769, 800, 1789, 2185
BbsI	GAAGAC	3 cut(s)	354, 1529, 2672
Bbv12I	GWGCWC	4 cut(s)	769, 800, 949, 2131
BbvCI	CCTCAGC	2 cut(s)	165, 1263
BbvI	GCAGC	5 cut(s)	405, 427, 1141, 2194, 2233
BceAI	ACGGC	3 cut(s)	370, 610, 658
BcgI	CGANNNNNNTGC	4 cut(s)	782, 816, 929, 963
BclI	TGATCA	3 cut(s)	540, 1434, 1539
BcoDI	GTCTC	6 cut(s)	499, 821, 947, 1032, 1180, 2292
BfaI	CTAG	6 cut(s)	888, 924, 1790, 1853, 2033, 2784
BfmI	CTRYAG	2 cut(s)	1155, 2595
BfuAI	ACCTGC	1 cut(s)	1283
BglI	GCCNNNNNGGC	1 cut(s)	626
BisI	GCNGC	9 cut(s)	358, 416, 419, 598, 1155, 1877, 2208, 2222, 2313
BlpI	GCTNAGC	1 cut(s)	711
BlsI	GCNGC	9 cut(s)	359, 417, 420, 599, 1156, 1878, 2209, 2223, 2314
Bme18I	GGWCC	1 cut(s)	518
BmgBI	CACGTC	1 cut(s)	2268
BmgT120I	GGNCC	3 cut(s)	518, 547, 599
BmiI	GGNNCC	2 cut(s)	601, 834
BmrI	ACTGGG	2 cut(s)	1576, 1998
BmsI	GCATC	7 cut(s)	949, 1291, 1811, 1935, 1982, 2208, 2414
BmuI	ACTGGG	2 cut(s)	1576, 1998
BpiI	GAAGAC	3 cut(s)	354, 1529, 2672
BplI	GAGNNNNNCTC	2 cut(s)	725, 757
BpmI	CTGGAG	1 cut(s)	1791
Bpu10I	CCTNAGC	2 cut(s)	165, 1263
Bpu1102I	GCTNAGC	1 cut(s)	711
BsaAI	YACGTR	2 cut(s)	1733, 2621
BsaBI	GATNNNNATC	1 cut(s)	539
BsaI	GGTCTC	1 cut(s)	499
BsaJI	CCNNGG	3 cut(s)	655, 2115, 2141
BsaWI	WCCGGW	1 cut(s)	2407
BsaXI	ACNNNNNCTCC	6 cut(s)	142, 145, 172, 175, 2659, 2689
Bsc4I	CCNNNNNNNGG	4 cut(s)	442, 661, 890, 2019
Bse1I	ACTGG	6 cut(s)	479, 848, 1169, 1571, 1993, 2609
Bse3DI	GCAATG	5 cut(s)	811, 1150, 1360, 2060, 2476
Bse8I	GATNNNNATC	1 cut(s)	539
BseDI	CCNNGG	3 cut(s)	655, 2115, 2141
BseGI	GGATG	7 cut(s)	1339, 1433, 1612, 1818, 1826, 1912, 2183
BseJI	GATNNNNATC	1 cut(s)	539
BseLI	CCNNNNNNNGG	4 cut(s)	442, 661, 890, 2019
BseMI	GCAATG	5 cut(s)	811, 1150, 1360, 2060, 2476
BseMII	CTCAG	4 cut(s)	179, 552, 702, 1254
BseNI	ACTGG	6 cut(s)	479, 848, 1169, 1571, 1993, 2609
BseXI	GCAGC	5 cut(s)	405, 427, 1141, 2194, 2233
Bsh1236I	CGCG	4 cut(s)	724, 951, 1680, 2395
BshFI	GGCC	6 cut(s)	549, 600, 1178, 2031, 2294, 2538
BsiHKAI	GWGCWC	4 cut(s)	769, 800, 949, 2131
BsiSI	CCGG	6 cut(s)	436, 508, 744, 836, 2295, 2408
BsiWI	CGTACG	1 cut(s)	1733
BslFI	GGGAC	2 cut(s)	218, 947
BslI	CCNNNNNNNGG	4 cut(s)	442, 661, 890, 2019
BsmAI	GTCTC	6 cut(s)	499, 821, 947, 1032, 1180, 2292
BsmBI	CGTCTC	1 cut(s)	821
BsmFI	GGGAC	2 cut(s)	218, 947
BsmI	GAATGC	1 cut(s)	1824
BsnI	GGCC	6 cut(s)	549, 600, 1178, 2031, 2294, 2538
Bso31I	GGTCTC	1 cut(s)	499
Bsp1286I	GDGCHC	6 cut(s)	769, 800, 949, 1789, 2131, 2185
Bsp143I	GATC	6 cut(s)	540, 1159, 1255, 1434, 1539, 1956
Bsp1720I	GCTNAGC	1 cut(s)	711
Bsp68I	TCGCGA	1 cut(s)	951
BspACI	CCGC	7 cut(s)	332, 357, 597, 629, 724, 1876, 2313
BspANI	GGCC	6 cut(s)	549, 600, 1178, 2031, 2294, 2538
BspCNI	CTCAG	4 cut(s)	178, 553, 703, 1255
BspFNI	CGCG	4 cut(s)	724, 951, 1680, 2395
BspHI	TCATGA	1 cut(s)	1959
BspLI	GGNNCC	2 cut(s)	601, 834
BspMAI	CTGCAG	2 cut(s)	1159, 2599
BspMI	ACCTGC	1 cut(s)	1283
BspPI	GGATC	1 cut(s)	1263
BspQI	GCTCTTC	1 cut(s)	1777
BspTNI	GGTCTC	1 cut(s)	499
BsrDI	GCAATG	5 cut(s)	811, 1150, 1360, 2060, 2476
BsrI	ACTGG	6 cut(s)	479, 848, 1169, 1571, 1993, 2609
BssECI	CCNNGG	3 cut(s)	655, 2115, 2141
BssMI	GATC	6 cut(s)	540, 1159, 1255, 1434, 1539, 1956
BssNAI	GTATAC	1 cut(s)	1003
BssT1I	CCWWGG	2 cut(s)	2115, 2141
Bst1107I	GTATAC	1 cut(s)	1003
Bst4CI	ACNGT	9 cut(s)	673, 752, 1112, 1693, 1834, 2496, 2512, 2581, 2726
Bst6I	CTCTTC	4 cut(s)	280, 372, 1777, 2182
BstBAI	YACGTR	2 cut(s)	1733, 2621
BstC8I	GCNNGC	6 cut(s)	308, 949, 1504, 1924, 2086, 2397
BstDEI	CTNAG	5 cut(s)	165, 561, 711, 774, 1263
BstDSI	CCRYGG	1 cut(s)	655
BstENI	CCTNNNNNAGG	1 cut(s)	888
BstF5I	GGATG	7 cut(s)	1339, 1433, 1612, 1818, 1826, 1912, 2183
BstFNI	CGCG	4 cut(s)	724, 951, 1680, 2395
BstHHI	GCGC	3 cut(s)	816, 1682, 2395
BstKTI	GATC	6 cut(s)	543, 1162, 1258, 1437, 1542, 1959
BstMAI	GTCTC	6 cut(s)	499, 821, 947, 1032, 1180, 2292
BstMBI	GATC	6 cut(s)	540, 1159, 1255, 1434, 1539, 1956
BstMWI	GCNNNNNNNGC	7 cut(s)	555, 626, 721, 1154, 1686, 2085, 2513
BstNSI	RCATGY	5 cut(s)	940, 1001, 1248, 2076, 2305
BstSFI	CTRYAG	2 cut(s)	1155, 2595
BstSNI	TACGTA	1 cut(s)	2621
BstUI	CGCG	4 cut(s)	724, 951, 1680, 2395
BstV1I	GCAGC	5 cut(s)	405, 427, 1141, 2194, 2233
BstV2I	GAAGAC	3 cut(s)	354, 1529, 2672
BstZ17I	GTATAC	1 cut(s)	1003
BsuRI	GGCC	6 cut(s)	549, 600, 1178, 2031, 2294, 2538
BtgI	CCRYGG	1 cut(s)	655
BtrI	CACGTC	1 cut(s)	2268
BtsCI	GGATG	7 cut(s)	1339, 1433, 1612, 1818, 1826, 1912, 2183
BtsIMutI	CAGTG	7 cut(s)	18, 558, 1162, 2375, 2492, 2602, 2731
BtuMI	TCGCGA	1 cut(s)	951
BveI	ACCTGC	1 cut(s)	1283
Cac8I	GCNNGC	6 cut(s)	308, 949, 1504, 1924, 2086, 2397
CciI	TCATGA	1 cut(s)	1959
CfoI	GCGC	3 cut(s)	816, 1682, 2395
Cfr13I	GGNCC	3 cut(s)	518, 547, 599
CpoI	CGGWCCG	1 cut(s)	518
CseI	GACGC	1 cut(s)	730
Csp6I	GTAC	6 cut(s)	391, 760, 1274, 1528, 1734, 2473
CspI	CGGWCCG	1 cut(s)	518
CviQI	GTAC	6 cut(s)	391, 760, 1274, 1528, 1734, 2473
DdeI	CTNAG	5 cut(s)	165, 561, 711, 774, 1263
DpnI	GATC	6 cut(s)	542, 1161, 1257, 1436, 1541, 1958
DpnII	GATC	6 cut(s)	540, 1159, 1255, 1434, 1539, 1956
DraI	TTTAAA	1 cut(s)	1200
Eam1104I	CTCTTC	4 cut(s)	280, 372, 1777, 2182
EarI	CTCTTC	4 cut(s)	280, 372, 1777, 2182
Ecl136II	GAGCTC	2 cut(s)	767, 798
Eco105I	TACGTA	1 cut(s)	2621
Eco130I	CCWWGG	2 cut(s)	2115, 2141
Eco24I	GRGCYC	4 cut(s)	769, 800, 1789, 2185
Eco31I	GGTCTC	1 cut(s)	499
Eco32I	GATATC	1 cut(s)	2254
Eco47I	GGWCC	1 cut(s)	518
Eco53kI	GAGCTC	2 cut(s)	767, 798
Eco57I	CTGAAG	3 cut(s)	1409, 2087, 2538
EcoICRI	GAGCTC	2 cut(s)	767, 798
EcoNI	CCTNNNNNAGG	1 cut(s)	888
EcoRV	GATATC	1 cut(s)	2254
EcoT14I	CCWWGG	2 cut(s)	2115, 2141
EcoT22I	ATGCAT	4 cut(s)	942, 1579, 1826, 1975
EcoT38I	GRGCYC	4 cut(s)	769, 800, 1789, 2185
ErhI	CCWWGG	2 cut(s)	2115, 2141
Esp3I	CGTCTC	1 cut(s)	821
FaqI	GGGAC	2 cut(s)	218, 947
FauNDI	CATATG	1 cut(s)	2053
FbaI	TGATCA	3 cut(s)	540, 1434, 1539
FblI	GTMKAC	2 cut(s)	1002, 2721
Fnu4HI	GCNGC	9 cut(s)	358, 416, 419, 598, 1155, 1877, 2208, 2222, 2313
FokI	GGATG	7 cut(s)	1346, 1440, 1619, 1825, 1833, 1919, 2190
FriOI	GRGCYC	4 cut(s)	769, 800, 1789, 2185
Fsp4HI	GCNGC	9 cut(s)	358, 416, 419, 598, 1155, 1877, 2208, 2222, 2313
FspBI	CTAG	6 cut(s)	888, 924, 1790, 1853, 2033, 2784
FspI	TGCGCA	1 cut(s)	815
GlaI	GCGC	3 cut(s)	815, 1681, 2394
GluI	GCNGC	9 cut(s)	358, 416, 419, 598, 1155, 1877, 2208, 2222, 2313
GsuI	CTGGAG	1 cut(s)	1791
HaeIII	GGCC	6 cut(s)	549, 600, 1178, 2031, 2294, 2538
HapII	CCGG	6 cut(s)	436, 508, 744, 836, 2295, 2408
HgaI	GACGC	1 cut(s)	730
HhaI	GCGC	3 cut(s)	816, 1682, 2395
Hin6I	GCGC	3 cut(s)	814, 1680, 2393
HinP1I	GCGC	3 cut(s)	814, 1680, 2393
HincII	GTYRAC	1 cut(s)	2740
HindII	GTYRAC	1 cut(s)	2740
HindIII	AAGCTT	2 cut(s)	1995, 2520
HinfI	GANTC	9 cut(s)	211, 263, 535, 582, 740, 1015, 1548, 1675, 2567
HpaII	CCGG	6 cut(s)	436, 508, 744, 836, 2295, 2408
Hpy166II	GTNNAC	6 cut(s)	18, 1003, 2473, 2602, 2722, 2740
Hpy188III	TCNNGA	7 cut(s)	208, 888, 950, 1552, 1960, 2571, 2678
Hpy8I	GTNNAC	6 cut(s)	18, 1003, 2473, 2602, 2722, 2740
Hpy99I	CGWCG	7 cut(s)	138, 356, 365, 368, 371, 752, 2720
HpyCH4III	ACNGT	9 cut(s)	673, 752, 1112, 1693, 1834, 2496, 2512, 2581, 2726
HpyCH4IV	ACGT	7 cut(s)	1526, 1732, 1736, 2267, 2620, 2718, 2799
HpyF10VI	GCNNNNNNNGC	7 cut(s)	555, 626, 721, 1154, 1686, 2085, 2513
HpyF3I	CTNAG	5 cut(s)	165, 561, 711, 774, 1263
HpySE526I	ACGT	7 cut(s)	1526, 1732, 1736, 2267, 2620, 2718, 2799
HspAI	GCGC	3 cut(s)	814, 1680, 2393
Ksp22I	TGATCA	3 cut(s)	540, 1434, 1539
Kzo9I	GATC	6 cut(s)	540, 1159, 1255, 1434, 1539, 1956
LguI	GCTCTTC	1 cut(s)	1777
LmnI	GCTCC	2 cut(s)	832, 1309
Lsp1109I	GCAGC	5 cut(s)	405, 427, 1141, 2194, 2233
LweI	GCATC	7 cut(s)	949, 1291, 1811, 1935, 1982, 2208, 2414
MaeI	CTAG	6 cut(s)	888, 924, 1790, 1853, 2033, 2784
MaeII	ACGT	7 cut(s)	1526, 1732, 1736, 2267, 2620, 2718, 2799
MaeIII	GTNAC	4 cut(s)	1102, 1337, 1667, 2110
MalI	GATC	6 cut(s)	542, 1161, 1257, 1436, 1541, 1958
MboI	GATC	6 cut(s)	540, 1159, 1255, 1434, 1539, 1956
MfeI	CAATTG	1 cut(s)	2322
MhlI	GDGCHC	6 cut(s)	769, 800, 949, 1789, 2131, 2185
MluCI	AATT	8 cut(s)	88, 463, 512, 1137, 2015, 2136, 2322, 2625
MlyI	GAGTC	1 cut(s)	734
MmeI	TCCRAC	4 cut(s)	660, 1435, 1853, 2739
Mph1103I	ATGCAT	4 cut(s)	942, 1579, 1826, 1975
MroXI	GAANNNNTTC	5 cut(s)	1462, 1997, 2150, 2241, 2669
MseI	TTAA	7 cut(s)	87, 675, 876, 1170, 1199, 1559, 2361
MslI	CAYNNNNRTG	4 cut(s)	1023, 1091, 1328, 2153
MspA1I	CMGCKG	1 cut(s)	418
MspI	CCGG	6 cut(s)	436, 508, 744, 836, 2295, 2408
MunI	CAATTG	1 cut(s)	2322
Mva1269I	GAATGC	1 cut(s)	1824
MvnI	CGCG	4 cut(s)	724, 951, 1680, 2395
MwoI	GCNNNNNNNGC	7 cut(s)	555, 626, 721, 1154, 1686, 2085, 2513
NdeI	CATATG	1 cut(s)	2053
NdeII	GATC	6 cut(s)	540, 1159, 1255, 1434, 1539, 1956
NlaIV	GGNNCC	2 cut(s)	601, 834
NmuCI	GTSAC	2 cut(s)	1102, 2110
NruI	TCGCGA	1 cut(s)	951
NsbI	TGCGCA	1 cut(s)	815
NsiI	ATGCAT	4 cut(s)	942, 1579, 1826, 1975
NspI	RCATGY	5 cut(s)	940, 1001, 1248, 2076, 2305
OliI	CACNNNNGTG	1 cut(s)	2153
PagI	TCATGA	1 cut(s)	1959
PciI	ACATGT	1 cut(s)	997
PciSI	GCTCTTC	1 cut(s)	1777
PcsI	WCGNNNNNNNCGW	3 cut(s)	357, 360, 1742
PctI	GAATGC	1 cut(s)	1824
PdmI	GAANNNNTTC	5 cut(s)	1462, 1997, 2150, 2241, 2669
PfeI	GAWTC	8 cut(s)	211, 263, 535, 582, 1015, 1548, 1675, 2567
Pfl23II	CGTACG	1 cut(s)	1733
PflFI	GACNNNGTC	1 cut(s)	613
PkrI	GCNGC	9 cut(s)	359, 417, 420, 599, 1156, 1878, 2209, 2223, 2314
PleI	GAGTC	1 cut(s)	734
PpsI	GAGTC	1 cut(s)	734
Ppu21I	YACGTR	2 cut(s)	1733, 2621
PscI	ACATGT	1 cut(s)	997
PsiI	TTATAA	1 cut(s)	1646
Psp124BI	GAGCTC	2 cut(s)	769, 800
Psp1406I	AACGTT	1 cut(s)	2799
PspLI	CGTACG	1 cut(s)	1733
PspN4I	GGNNCC	2 cut(s)	601, 834
PspPI	GGNCC	3 cut(s)	518, 547, 599
PstI	CTGCAG	2 cut(s)	1159, 2599
PsyI	GACNNNGTC	1 cut(s)	613
PvuII	CAGCTG	1 cut(s)	418
RruI	TCGCGA	1 cut(s)	951
RsaI	GTAC	6 cut(s)	392, 761, 1275, 1529, 1735, 2474
RsaNI	GTAC	6 cut(s)	391, 760, 1274, 1528, 1734, 2473
RseI	CAYNNNNRTG	4 cut(s)	1023, 1091, 1328, 2153
Rsr2I	CGGWCCG	1 cut(s)	518
RsrII	CGGWCCG	1 cut(s)	518
SacI	GAGCTC	2 cut(s)	769, 800
SapI	GCTCTTC	1 cut(s)	1777
SaqAI	TTAA	7 cut(s)	87, 675, 876, 1170, 1199, 1559, 2361
SatI	GCNGC	9 cut(s)	358, 416, 419, 598, 1155, 1877, 2208, 2222, 2313
Sau3AI	GATC	6 cut(s)	540, 1159, 1255, 1434, 1539, 1956
Sau96I	GGNCC	3 cut(s)	518, 547, 599
SchI	GAGTC	1 cut(s)	734
SduI	GDGCHC	6 cut(s)	769, 800, 949, 1789, 2131, 2185
SfaNI	GCATC	7 cut(s)	949, 1291, 1811, 1935, 1982, 2208, 2414
SfcI	CTRYAG	2 cut(s)	1155, 2595
SinI	GGWCC	1 cut(s)	518
SmiMI	CAYNNNNRTG	4 cut(s)	1023, 1091, 1328, 2153
SnaBI	TACGTA	1 cut(s)	2621
Sse9I	AATT	8 cut(s)	88, 463, 512, 1137, 2015, 2136, 2322, 2625
SsiI	CCGC	7 cut(s)	332, 357, 597, 629, 724, 1876, 2313
SspI	AATATT	2 cut(s)	1222, 1902
SspMI	CTAG	6 cut(s)	888, 924, 1790, 1853, 2033, 2784
SstI	GAGCTC	2 cut(s)	769, 800
StyI	CCWWGG	2 cut(s)	2115, 2141
TaaI	ACNGT	9 cut(s)	673, 752, 1112, 1693, 1834, 2496, 2512, 2581, 2726
TaiI	ACGT	7 cut(s)	1529, 1735, 1739, 2270, 2623, 2721, 2802
TaqI	TCGA	6 cut(s)	429, 1258, 1311, 2001, 2185, 2609
TaqII	GACCGA	2 cut(s)	535, 759
TasI	AATT	8 cut(s)	88, 463, 512, 1137, 2015, 2136, 2322, 2625
TatI	WGTACW	1 cut(s)	390
TauI	GCSGC	4 cut(s)	360, 600, 1879, 2315
TfiI	GAWTC	8 cut(s)	211, 263, 535, 582, 1015, 1548, 1675, 2567
Tru1I	TTAA	7 cut(s)	87, 675, 876, 1170, 1199, 1559, 2361
Tru9I	TTAA	7 cut(s)	87, 675, 876, 1170, 1199, 1559, 2361
TscAI	CASTG	7 cut(s)	18, 565, 1169, 2382, 2499, 2609, 2731
TseFI	GTSAC	2 cut(s)	1102, 2110
TseI	GCWGC	5 cut(s)	415, 418, 1154, 2207, 2221
Tsp45I	GTSAC	2 cut(s)	1102, 2110
TspGWI	ACGGA	1 cut(s)	845
TspRI	CASTG	7 cut(s)	18, 565, 1169, 2382, 2499, 2609, 2731
Tth111I	GACNNNGTC	1 cut(s)	613
VpaK11BI	GGWCC	1 cut(s)	518
XagI	CCTNNNNNAGG	1 cut(s)	888
XapI	RAATTY	2 cut(s)	463, 2136
XbaI	TCTAGA	1 cut(s)	887
XceI	RCATGY	5 cut(s)	940, 1001, 1248, 2076, 2305
XcmI	CCANNNNNNNNNTGG	1 cut(s)	628
XmiI	GTMKAC	2 cut(s)	1002, 2721
XmnI	GAANNNNTTC	5 cut(s)	1462, 1997, 2150, 2241, 2669
XspI	CTAG	6 cut(s)	888, 924, 1790, 1853, 2033, 2784
Zsp2I	ATGCAT	4 cut(s)	942, 1579, 1826, 1975

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.