RchiOBHm_Chr1g0336641

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0012039
Physical Location & Seq
Reverse (-)
28366732 .. 28371640
4909 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56458

Sequence Viewer

Length: 591 bp
ATGCAGCCAGGTTTGGTGGAGGAGGGACTAAATCTTTTTGATGAGCTTCTTAGAGATGGGTCAATACAAGTGAGGGAAGAGCATTACACTTGCTTAGTTGATCTTTGTGGTCGAGCAGGAAGGCTGAAGGAGGCTTTTGGTATCCTCGAGAAGCTTGCGACCAACCCATCAGCATCTAAGTGGGGCGCTCTTCTTGCTGGATTTATTGATCATCATAACATGGATACCGGGAAGCTAGCTGCAGAGAAGCTCTTAAAGGAGGGACCAGATGATGCAGGCAGGTACTCGTTGTTGTGGAATTTTTTTGCTAGATCTGGGAATTGGAGAGAAGCTGCAAAGATGAGGATAAAAATGAAAGAGAAGGGGTTCAAAAAGCAGCCCGGTTGCAGTTGGATAGAAGTTGGAAATATGGTGCATGTGTTTGTGGTTGGTGATAAGTCTCATTGCCAATCTCAACTTATATATTCTGCACTTTACGATTTACATAAACAAATGAAGAAGATTGGGTATATACCAAGCAATGATTTGAATGTACATGAAGATTTTTCAATTACAGGAATTCTTACTTGGGATATCTTCAGACTTATATAG

Protein Analysis

196

Amino Acids

22.2

Weight (kDa)

7.05

Isoelectric Point (pI)

25.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E_motif PF20431 71 - 133 2.2e-17 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 270
AcsI RAATTY 2 cut(s) 298, 558
AcuI CTGAAG 2 cut(s) 146, 562
AfaI GTAC 2 cut(s) 284, 534
AgsI TTSAA 3 cut(s) 370, 529, 549
AjnI CCWGG 1 cut(s) 7
AjuI GAANNNNNNNTTGG 2 cut(s) 550, 582
AluBI AGCT 6 cut(s) 46, 154, 235, 239, 250, 332
AluI AGCT 6 cut(s) 46, 154, 235, 239, 250, 332
Alw26I GTCTC 1 cut(s) 444
Ama87I CYCGRG 1 cut(s) 146
ApeKI GCWGC 4 cut(s) 4, 239, 332, 376
ApoI RAATTY 2 cut(s) 298, 558
Asp700I GAANNNNTTC 1 cut(s) 365
AspLEI GCGC 1 cut(s) 188
AspS9I GGNCC 1 cut(s) 263
AsuC2I CCSGG 2 cut(s) 229, 381
AsuHPI GGTGA 1 cut(s) 443
AsuNHI GCTAGC 1 cut(s) 235
AvaI CYCGRG 1 cut(s) 146
AvaII GGWCC 1 cut(s) 263
BbvI GCAGC 4 cut(s) 16, 226, 319, 388
BccI CCATC 2 cut(s) 50, 175
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BciT130I CCWGG 1 cut(s) 9
BciVI GTATCC 2 cut(s) 152, 217
BclI TGATCA 1 cut(s) 208
BcnI CCSGG 2 cut(s) 229, 381
BcoDI GTCTC 1 cut(s) 444
BfaI CTAG 2 cut(s) 236, 309
BfmI CTRYAG 1 cut(s) 240
BfoI RGCGCY 1 cut(s) 189
BfuAI ACCTGC 1 cut(s) 270
BfuI GTATCC 2 cut(s) 152, 217
BglII AGATCT 1 cut(s) 311
BisI GCNGC 4 cut(s) 5, 240, 333, 377
BlsI GCNGC 4 cut(s) 6, 241, 334, 378
Bme1390I CCNGG 3 cut(s) 9, 229, 381
Bme18I GGWCC 1 cut(s) 263
BmeT110I CYCGRG 1 cut(s) 146
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 1 cut(s) 264
BmrFI CCNGG 3 cut(s) 9, 229, 381
BmsI GCATC 2 cut(s) 182, 262
BmtI GCTAGC 1 cut(s) 239
BpuMI CCSGG 2 cut(s) 229, 381
Bse3DI GCAATG 2 cut(s) 442, 526
BseBI CCWGG 1 cut(s) 9
BseMI GCAATG 2 cut(s) 442, 526
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 4 cut(s) 16, 226, 319, 388
BsgI GTGCAG 1 cut(s) 453
BsiHKCI CYCGRG 1 cut(s) 146
BsiSI CCGG 2 cut(s) 228, 381
BslFI GGGAC 2 cut(s) 39, 276
BsmAI GTCTC 1 cut(s) 444
BsmFI GGGAC 2 cut(s) 39, 276
BsoBI CYCGRG 1 cut(s) 146
Bsp1407I TGTACA 1 cut(s) 532
Bsp143I GATC 3 cut(s) 100, 208, 311
BspLI GGNNCC 1 cut(s) 264
BspMAI CTGCAG 1 cut(s) 244
BspMI ACCTGC 1 cut(s) 270
BspOI GCTAGC 1 cut(s) 239
BspQI GCTCTTC 2 cut(s) 72, 195
BsrDI GCAATG 2 cut(s) 442, 526
BsrGI TGTACA 1 cut(s) 532
BssMI GATC 3 cut(s) 100, 208, 311
Bst2UI CCWGG 1 cut(s) 9
Bst6I CTCTTC 2 cut(s) 72, 195
BstAUI TGTACA 1 cut(s) 532
BstC8I GCNNGC 3 cut(s) 156, 237, 277
BstDEI CTNAG 3 cut(s) 50, 94, 177
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 1 cut(s) 188
BstKTI GATC 3 cut(s) 103, 211, 314
BstMAI GTCTC 1 cut(s) 444
BstMBI GATC 3 cut(s) 100, 208, 311
BstMWI GCNNNNNNNGC 1 cut(s) 194
BstNI CCWGG 1 cut(s) 9
BstNSI RCATGY 1 cut(s) 419
BstSCI CCNGG 3 cut(s) 7, 227, 379
BstSFI CTRYAG 1 cut(s) 240
BstV1I GCAGC 4 cut(s) 16, 226, 319, 388
BstX2I RGATCY 1 cut(s) 311
BstYI RGATCY 1 cut(s) 311
BsuI GTATCC 2 cut(s) 152, 217
BveI ACCTGC 1 cut(s) 270
Cac8I GCNNGC 3 cut(s) 156, 237, 277
CfoI GCGC 1 cut(s) 188
Cfr13I GGNCC 1 cut(s) 263
Csp6I GTAC 2 cut(s) 283, 533
CviAII CATG 3 cut(s) 220, 416, 536
CviQI GTAC 2 cut(s) 283, 533
DdeI CTNAG 3 cut(s) 50, 94, 177
DpnI GATC 3 cut(s) 102, 210, 313
DpnII GATC 3 cut(s) 100, 208, 311
Eam1104I CTCTTC 2 cut(s) 72, 195
EarI CTCTTC 2 cut(s) 72, 195
Eco32I GATATC 1 cut(s) 574
Eco47I GGWCC 1 cut(s) 263
Eco57I CTGAAG 2 cut(s) 146, 562
Eco88I CYCGRG 1 cut(s) 146
EcoRI GAATTC 1 cut(s) 558
EcoRII CCWGG 1 cut(s) 7
EcoRV GATATC 1 cut(s) 574
FaeI CATG 3 cut(s) 223, 419, 539
FaqI GGGAC 2 cut(s) 39, 276
FatI CATG 3 cut(s) 219, 415, 535
FbaI TGATCA 1 cut(s) 208
Fnu4HI GCNGC 4 cut(s) 5, 240, 333, 377
Fsp4HI GCNGC 4 cut(s) 5, 240, 333, 377
FspBI CTAG 2 cut(s) 236, 309
GlaI GCGC 1 cut(s) 187
GluI GCNGC 4 cut(s) 5, 240, 333, 377
HaeII RGCGCY 1 cut(s) 189
HapII CCGG 2 cut(s) 228, 381
HhaI GCGC 1 cut(s) 188
Hin1II CATG 3 cut(s) 223, 419, 539
Hin6I GCGC 1 cut(s) 186
HinP1I GCGC 1 cut(s) 186
HindIII AAGCTT 1 cut(s) 152
HpaII CCGG 2 cut(s) 228, 381
HphI GGTGA 1 cut(s) 443
Hpy188I TCNGA 1 cut(s) 581
Hpy188III TCNNGA 1 cut(s) 148
HpyAV CCTTC 3 cut(s) 114, 121, 355
HpyCH4V TGCA 7 cut(s) 4, 242, 275, 335, 387, 415, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 194
HpyF3I CTNAG 3 cut(s) 50, 94, 177
Hsp92II CATG 3 cut(s) 223, 419, 539
HspAI GCGC 1 cut(s) 186
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 3 cut(s) 100, 208, 311
LguI GCTCTTC 2 cut(s) 72, 195
Lsp1109I GCAGC 4 cut(s) 16, 226, 319, 388
LweI GCATC 2 cut(s) 182, 262
MaeI CTAG 2 cut(s) 236, 309
MalI GATC 3 cut(s) 102, 210, 313
MboI GATC 3 cut(s) 100, 208, 311
MboII GAAGA 6 cut(s) 89, 182, 508, 511, 551, 568
MflI RGATCY 1 cut(s) 311
MluCI AATT 4 cut(s) 298, 319, 549, 558
MmeI TCCRAC 2 cut(s) 371, 382
MnlI CCTC 7 cut(s) 13, 16, 66, 124, 155, 253, 336
MroXI GAANNNNTTC 1 cut(s) 365
MseI TTAA 1 cut(s) 254
MslI CAYNNNNRTG 1 cut(s) 178
MspI CCGG 2 cut(s) 228, 381
MspR9I CCNGG 3 cut(s) 9, 229, 381
MvaI CCWGG 1 cut(s) 9
MwoI GCNNNNNNNGC 1 cut(s) 194
NciI CCSGG 2 cut(s) 229, 381
NdeII GATC 3 cut(s) 100, 208, 311
NheI GCTAGC 1 cut(s) 235
NlaIII CATG 3 cut(s) 223, 419, 539
NlaIV GGNNCC 1 cut(s) 264
NspI RCATGY 1 cut(s) 419
PaeR7I CTCGAG 1 cut(s) 146
PciSI GCTCTTC 2 cut(s) 72, 195
PdmI GAANNNNTTC 1 cut(s) 365
PkrI GCNGC 4 cut(s) 6, 241, 334, 378
Psp6I CCWGG 1 cut(s) 7
PspGI CCWGG 1 cut(s) 7
PspN4I GGNNCC 1 cut(s) 264
PspPI GGNCC 1 cut(s) 263
PstI CTGCAG 1 cut(s) 244
PsuI RGATCY 1 cut(s) 311
RsaI GTAC 2 cut(s) 284, 534
RsaNI GTAC 2 cut(s) 283, 533
RseI CAYNNNNRTG 1 cut(s) 178
SapI GCTCTTC 2 cut(s) 72, 195
SaqAI TTAA 1 cut(s) 254
SatI GCNGC 4 cut(s) 5, 240, 333, 377
Sau3AI GATC 3 cut(s) 100, 208, 311
Sau96I GGNCC 1 cut(s) 263
ScrFI CCNGG 3 cut(s) 9, 229, 381
SetI ASST 8 cut(s) 13, 48, 156, 237, 241, 252, 284, 334
SfaNI GCATC 2 cut(s) 182, 262
SfcI CTRYAG 1 cut(s) 240
Sfr274I CTCGAG 1 cut(s) 146
SinI GGWCC 1 cut(s) 263
SlaI CTCGAG 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 178
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 4 cut(s) 298, 319, 549, 558
SspMI CTAG 2 cut(s) 236, 309
StyD4I CCNGG 3 cut(s) 7, 227, 379
TaqI TCGA 2 cut(s) 112, 147
TasI AATT 4 cut(s) 298, 319, 549, 558
TatI WGTACW 1 cut(s) 532
Tru1I TTAA 1 cut(s) 254
Tru9I TTAA 1 cut(s) 254
TseI GCWGC 4 cut(s) 4, 239, 332, 376
TspDTI ATGAA 3 cut(s) 368, 509, 552
VpaK11BI GGWCC 1 cut(s) 263
XapI RAATTY 2 cut(s) 298, 558
XceI RCATGY 1 cut(s) 419
XhoI CTCGAG 1 cut(s) 146
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 2 cut(s) 236, 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.