RchiOBHm_Chr1g0339511

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003834
Physical Location & Seq
Forward (+)
30945226 .. 30949788
4563 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56642

Sequence Viewer

Length: 1200 bp
ATGCTGTCTCCAATGGGATTGAGAAGACCCCCAAAGCCCAAATCTGGGCCCAGTTCCCCCATCATCTTCCTCGCCATCTGCATAGCCGCAATTGCTCTCGTCTTCCTCTATTCCTATCTCAGTTCCACCAATGCCTTCTCTTCTTCCTCCCTCAAAACCCTACAGACTGTCCCCAATTTGATCAACCACAGCCACGAGAAGTACCTCTACTGGGGTACCCGAATCGACTGCCCCGGCAAGCACTGCCAGTCCTGTGAGGGCCTCGCCCACCAAGAGTCCAGCCTCCGCTGTGCTCTTGAAGAAGCCATCTTCCTCCGCAGGACTTTTGTAATGCCTTCCAGAATGTGCATCAATCCAATACACAATAAGAAAGGGATCTTTCATCACTCGGATAATGCAAGTTCAGAAGAAAATTGGGATGCAAACTCTTGTTCCATGGACTCTTTGTATGATATGGACTTCATATCGAAGACTGTACCAGTAATTTTAGACGATTCAAAAATGTGGTATCAGGTGCTAACAACAAGCATGAAGTTGGGAGATAGAGGAGTTTCTCATGTGGAACGAGTTAGTCGTGTTGATCTCAAAAACAGTAGTTTCTCAAATCTTTTGCTGATAAACAGAACAGCAAGCCCTCTTTCATGGTTTGCCGAGTGCAAGAATCGAAACAACCATAGTGCTTTAATTTTGCCCTACTCCTTTCTCCCCTCGAGGGCAGCAAAGAAACTGAGGAATGCAGCTGAGAAGATTAAGGCAATCCTTGGTGATTATGATGCCATTCATGTTCGTCGTGGAGATAGACTTAAGACCAGGAGGGACAGGAATGGTGTTGAGAGGACCCTTTCACCTCATCTGGACAGGGACACACGCCCTGAGTTTATTCTCCGCAGAATTAAAAAATGGGTCCCACCTGGACGAACTCTTTACATTGCTTCAAATGAAAGGACACCAGGATTCTTTTCACCTCTCTCAGTCAGATACAAATTGGCATACTCATCAAATTATAGCCACATGTTGGAACCCTTGATTGAGAATAACTACCAATTGTTCATGATTGAAAGGCTTATCTGGATGGGTGCTAAAACATTCATTAGCACATTCAAAGAAGATGATACAGATCTCCATCTTACGGACGACCCCAAGAAGAACACCAAGAACTGGCAAATACCAGTCTACACCATGGATGAAGAGGGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

399

Amino Acids

45.72

Weight (kDa)

9.31

Isoelectric Point (pI)

50.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 214 - 366 8.6e-06 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 709
Acc65I GGTACC 1 cut(s) 215
AccB1I GGYRCC 1 cut(s) 215
AccB7I CCANNNNNTGG 2 cut(s) 1015, 1158
AccI GTMKAC 1 cut(s) 1173
AciI CCGC 4 cut(s) 87, 286, 316, 886
AclWI GGATC 1 cut(s) 383
AfaI GTAC 3 cut(s) 203, 217, 477
AfiI CCNNNNNNNGG 7 cut(s) 44, 45, 211, 712, 1015, 1129, 1158
AflII CTTAAG 1 cut(s) 803
AflIII ACRYGT 1 cut(s) 1011
AgsI TTSAA 5 cut(s) 299, 498, 936, 1058, 1102
AjnI CCWGG 3 cut(s) 809, 910, 949
AluBI AGCT 2 cut(s) 740, 1197
AluI AGCT 2 cut(s) 740, 1197
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 1 cut(s) 12
AlwI GGATC 1 cut(s) 383
Ama87I CYCGRG 1 cut(s) 709
AoxI GGCC 2 cut(s) 47, 259
ApaI GGGCCC 1 cut(s) 51
ApeKI GCWGC 2 cut(s) 716, 737
Asp718I GGTACC 1 cut(s) 215
AspS9I GGNCC 5 cut(s) 47, 48, 259, 837, 904
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 3 cut(s) 776, 837, 954
AvaI CYCGRG 1 cut(s) 709
AvaII GGWCC 2 cut(s) 837, 904
BaeGI GKGCMC 1 cut(s) 51
BaeI ACNNNNGTAYC 2 cut(s) 199, 232
BanI GGYRCC 1 cut(s) 215
BanII GRGCYC 1 cut(s) 51
BarI GAAGNNNNNNTAC 2 cut(s) 191, 223
BauI CACGAG 1 cut(s) 194
BbsI GAAGAC 3 cut(s) 31, 94, 476
Bbv12I GWGCWC 1 cut(s) 295
BbvI GCAGC 2 cut(s) 728, 749
BccI CCATC 5 cut(s) 68, 83, 314, 1066, 1131
BcgI CGANNNNNNTGC 2 cut(s) 210, 244
BciT130I CCWGG 3 cut(s) 811, 912, 951
BclI TGATCA 1 cut(s) 180
BcnI CCSGG 1 cut(s) 234
BcoDI GTCTC 1 cut(s) 12
BfmI CTRYAG 1 cut(s) 161
BfrI CTTAAG 1 cut(s) 803
BglII AGATCT 1 cut(s) 1117
BisI GCNGC 3 cut(s) 87, 717, 738
BlsI GCNGC 3 cut(s) 88, 718, 739
Bme1390I CCNGG 4 cut(s) 234, 811, 912, 951
Bme18I GGWCC 2 cut(s) 837, 904
BmeT110I CYCGRG 1 cut(s) 709
BmgT120I GGNCC 5 cut(s) 47, 48, 259, 837, 904
BmiI GGNNCC 6 cut(s) 49, 217, 839, 905, 906, 1020
BmrFI CCNGG 4 cut(s) 234, 811, 912, 951
BmrI ACTGGG 2 cut(s) 45, 220
BmsI GCATC 3 cut(s) 357, 409, 763
BmuI ACTGGG 2 cut(s) 45, 220
BpiI GAAGAC 3 cut(s) 31, 94, 476
BpuMI CCSGG 1 cut(s) 234
BsaBI GATNNNNATC 2 cut(s) 1116, 1122
BsaJI CCNNGG 4 cut(s) 232, 435, 760, 1179
Bsc4I CCNNNNNNNGG 7 cut(s) 44, 45, 211, 712, 1015, 1129, 1158
Bse1I ACTGG 6 cut(s) 51, 215, 247, 479, 1163, 1169
Bse3DI GCAATG 1 cut(s) 927
Bse8I GATNNNNATC 2 cut(s) 1116, 1122
BseBI CCWGG 3 cut(s) 811, 912, 951
BseDI CCNNGG 4 cut(s) 232, 435, 760, 1179
BseGI GGATG 3 cut(s) 424, 1077, 1189
BseJI GATNNNNATC 2 cut(s) 1116, 1122
BseLI CCNNNNNNNGG 7 cut(s) 44, 45, 211, 712, 1015, 1129, 1158
BseMI GCAATG 1 cut(s) 927
BseMII CTCAG 5 cut(s) 133, 719, 732, 864, 984
BseNI ACTGG 6 cut(s) 51, 215, 247, 479, 1163, 1169
BseRI GAGGAG 1 cut(s) 561
BseSI GKGCMC 1 cut(s) 51
BseXI GCAGC 2 cut(s) 728, 749
BshFI GGCC 2 cut(s) 49, 261
BshNI GGYRCC 1 cut(s) 215
BsiHKAI GWGCWC 1 cut(s) 295
BsiHKCI CYCGRG 1 cut(s) 709
BsiSI CCGG 1 cut(s) 234
BslFI GGGAC 4 cut(s) 155, 830, 875, 890
BslI CCNNNNNNNGG 7 cut(s) 44, 45, 211, 712, 1015, 1129, 1158
BsmAI GTCTC 1 cut(s) 12
BsmFI GGGAC 4 cut(s) 155, 830, 875, 890
BsmI GAATGC 1 cut(s) 739
BsnI GGCC 2 cut(s) 49, 261
BsoBI CYCGRG 1 cut(s) 709
Bsp120I GGGCCC 1 cut(s) 47
Bsp1286I GDGCHC 2 cut(s) 51, 295
Bsp143I GATC 4 cut(s) 180, 375, 580, 1117
Bsp19I CCATGG 2 cut(s) 435, 1179
BspACI CCGC 4 cut(s) 87, 286, 316, 886
BspANI GGCC 2 cut(s) 49, 261
BspCNI CTCAG 5 cut(s) 132, 720, 733, 865, 983
BspHI TCATGA 1 cut(s) 1050
BspLI GGNNCC 6 cut(s) 49, 217, 839, 905, 906, 1020
BspPI GGATC 1 cut(s) 383
BspT107I GGYRCC 1 cut(s) 215
BspTI CTTAAG 1 cut(s) 803
BsrDI GCAATG 1 cut(s) 927
BsrI ACTGG 6 cut(s) 51, 215, 247, 479, 1163, 1169
BssECI CCNNGG 4 cut(s) 232, 435, 760, 1179
BssMI GATC 4 cut(s) 180, 375, 580, 1117
BssSI CACGAG 1 cut(s) 194
BssT1I CCWWGG 3 cut(s) 435, 760, 1179
Bst2BI CACGAG 1 cut(s) 194
Bst2UI CCWGG 3 cut(s) 811, 912, 951
Bst4CI ACNGT 3 cut(s) 169, 475, 593
Bst6I CTCTTC 2 cut(s) 145, 1182
BstAFI CTTAAG 1 cut(s) 803
BstAPI GCANNNNNTGC 1 cut(s) 243
BstC8I GCNNGC 2 cut(s) 239, 631
BstDEI CTNAG 5 cut(s) 119, 728, 741, 873, 970
BstDSI CCRYGG 2 cut(s) 435, 1179
BstF5I GGATG 3 cut(s) 424, 1077, 1189
BstKTI GATC 4 cut(s) 183, 378, 583, 1120
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 4 cut(s) 180, 375, 580, 1117
BstMWI GCNNNNNNNGC 2 cut(s) 92, 243
BstNI CCWGG 3 cut(s) 811, 912, 951
BstNSI RCATGY 1 cut(s) 1015
BstSCI CCNGG 4 cut(s) 232, 809, 910, 949
BstSFI CTRYAG 1 cut(s) 161
BstSLI GKGCMC 1 cut(s) 51
BstV1I GCAGC 2 cut(s) 728, 749
BstV2I GAAGAC 3 cut(s) 31, 94, 476
BstX2I RGATCY 2 cut(s) 375, 1117
BstYI RGATCY 2 cut(s) 375, 1117
BsuRI GGCC 2 cut(s) 49, 261
BtgI CCRYGG 2 cut(s) 435, 1179
BtsCI GGATG 3 cut(s) 424, 1077, 1189
BtsI GCAGTG 1 cut(s) 241
BtsIMutI CAGTG 1 cut(s) 241
Cac8I GCNNGC 2 cut(s) 239, 631
CciI TCATGA 1 cut(s) 1050
Cfr13I GGNCC 5 cut(s) 47, 48, 259, 837, 904
Csp6I GTAC 3 cut(s) 202, 216, 476
CviAII CATG 8 cut(s) 436, 529, 557, 642, 782, 1012, 1051, 1180
CviQI GTAC 3 cut(s) 202, 216, 476
DdeI CTNAG 5 cut(s) 119, 728, 741, 873, 970
DpnI GATC 4 cut(s) 182, 377, 582, 1119
DpnII GATC 4 cut(s) 180, 375, 580, 1117
Eam1104I CTCTTC 2 cut(s) 145, 1182
EarI CTCTTC 2 cut(s) 145, 1182
Eco130I CCWWGG 3 cut(s) 435, 760, 1179
Eco24I GRGCYC 1 cut(s) 51
Eco47I GGWCC 2 cut(s) 837, 904
Eco88I CYCGRG 1 cut(s) 709
EcoO109I RGGNCCY 3 cut(s) 259, 837, 904
EcoRII CCWGG 3 cut(s) 809, 910, 949
EcoT14I CCWWGG 3 cut(s) 435, 760, 1179
EcoT38I GRGCYC 1 cut(s) 51
ErhI CCWWGG 3 cut(s) 435, 760, 1179
FaeI CATG 8 cut(s) 439, 532, 560, 645, 785, 1015, 1054, 1183
FaqI GGGAC 4 cut(s) 155, 830, 875, 890
FatI CATG 8 cut(s) 435, 528, 556, 641, 781, 1011, 1050, 1179
FbaI TGATCA 1 cut(s) 180
FblI GTMKAC 1 cut(s) 1173
Fnu4HI GCNGC 3 cut(s) 87, 717, 738
FokI GGATG 3 cut(s) 431, 1084, 1196
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 3 cut(s) 87, 717, 738
GluI GCNGC 3 cut(s) 87, 717, 738
HaeIII GGCC 2 cut(s) 49, 261
HapII CCGG 1 cut(s) 234
Hin1II CATG 8 cut(s) 439, 532, 560, 645, 785, 1015, 1054, 1183
HinfI GANTC 6 cut(s) 222, 275, 440, 494, 661, 954
HpaII CCGG 1 cut(s) 234
HphI GGTGA 3 cut(s) 776, 837, 954
Hpy166II GTNNAC 1 cut(s) 1174
Hpy188I TCNGA 3 cut(s) 391, 406, 977
Hpy188III TCNNGA 5 cut(s) 296, 339, 854, 1051, 1069
Hpy8I GTNNAC 1 cut(s) 1174
Hpy99I CGWCG 1 cut(s) 792
HpyAV CCTTC 2 cut(s) 145, 345
HpyCH4III ACNGT 3 cut(s) 169, 475, 593
HpyCH4V TGCA 6 cut(s) 81, 348, 398, 422, 657, 737
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 243
HpyF3I CTNAG 5 cut(s) 119, 728, 741, 873, 970
Hsp92II CATG 8 cut(s) 439, 532, 560, 645, 785, 1015, 1054, 1183
KflI GGGWCCC 1 cut(s) 904
KpnI GGTACC 1 cut(s) 219
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 4 cut(s) 180, 375, 580, 1117
Lsp1109I GCAGC 2 cut(s) 728, 749
LweI GCATC 3 cut(s) 357, 409, 763
MalI GATC 4 cut(s) 182, 377, 582, 1119
MboI GATC 4 cut(s) 180, 375, 580, 1117
MfeI CAATTG 2 cut(s) 90, 1043
MflI RGATCY 2 cut(s) 375, 1117
MhlI GDGCHC 2 cut(s) 51, 295
MluCI AATT 9 cut(s) 90, 175, 412, 483, 684, 891, 983, 1000, 1043
MlyI GAGTC 2 cut(s) 284, 434
MmeI TCCRAC 1 cut(s) 996
MseI TTAA 4 cut(s) 683, 750, 804, 894
MspA1I CMGCKG 2 cut(s) 288, 740
MspCI CTTAAG 1 cut(s) 803
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 4 cut(s) 234, 811, 912, 951
MunI CAATTG 2 cut(s) 90, 1043
Mva1269I GAATGC 1 cut(s) 739
MvaI CCWGG 3 cut(s) 811, 912, 951
MwoI GCNNNNNNNGC 2 cut(s) 92, 243
NciI CCSGG 1 cut(s) 234
NcoI CCATGG 2 cut(s) 435, 1179
NdeII GATC 4 cut(s) 180, 375, 580, 1117
NlaIII CATG 8 cut(s) 439, 532, 560, 645, 785, 1015, 1054, 1183
NlaIV GGNNCC 6 cut(s) 49, 217, 839, 905, 906, 1020
NmeAIII GCCGAG 1 cut(s) 676
NspI RCATGY 1 cut(s) 1015
PaeR7I CTCGAG 1 cut(s) 709
PagI TCATGA 1 cut(s) 1050
PciI ACATGT 1 cut(s) 1011
PcsI WCGNNNNNNNCGW 1 cut(s) 571
PctI GAATGC 1 cut(s) 739
PfeI GAWTC 4 cut(s) 222, 494, 661, 954
PflMI CCANNNNNTGG 2 cut(s) 1015, 1158
PkrI GCNGC 3 cut(s) 88, 718, 739
PleI GAGTC 2 cut(s) 283, 434
PpsI GAGTC 2 cut(s) 283, 434
PpuMI RGGWCCY 2 cut(s) 837, 904
PscI ACATGT 1 cut(s) 1011
Psp5II RGGWCCY 2 cut(s) 837, 904
Psp6I CCWGG 3 cut(s) 809, 910, 949
PspGI CCWGG 3 cut(s) 809, 910, 949
PspN4I GGNNCC 6 cut(s) 49, 217, 839, 905, 906, 1020
PspOMI GGGCCC 1 cut(s) 47
PspPI GGNCC 5 cut(s) 47, 48, 259, 837, 904
PspPPI RGGWCCY 2 cut(s) 837, 904
PspXI VCTCGAGB 1 cut(s) 709
PsuI RGATCY 2 cut(s) 375, 1117
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 3 cut(s) 203, 217, 477
RsaNI GTAC 3 cut(s) 202, 216, 476
SaqAI TTAA 4 cut(s) 683, 750, 804, 894
SatI GCNGC 3 cut(s) 87, 717, 738
Sau3AI GATC 4 cut(s) 180, 375, 580, 1117
Sau96I GGNCC 5 cut(s) 47, 48, 259, 837, 904
SchI GAGTC 2 cut(s) 284, 434
ScrFI CCNGG 4 cut(s) 234, 811, 912, 951
SduI GDGCHC 2 cut(s) 51, 295
SetI ASST 7 cut(s) 207, 516, 742, 850, 913, 967, 1199
SfaNI GCATC 3 cut(s) 357, 409, 763
SfcI CTRYAG 1 cut(s) 161
Sfr274I CTCGAG 1 cut(s) 709
SinI GGWCC 2 cut(s) 837, 904
SlaI CTCGAG 1 cut(s) 709
SmlI CTYRAG 2 cut(s) 709, 803
SmoI CTYRAG 2 cut(s) 709, 803
Sse9I AATT 9 cut(s) 90, 175, 412, 483, 684, 891, 983, 1000, 1043
SsiI CCGC 4 cut(s) 87, 286, 316, 886
StyD4I CCNGG 4 cut(s) 232, 809, 910, 949
StyI CCWWGG 3 cut(s) 435, 760, 1179
TaaI ACNGT 3 cut(s) 169, 475, 593
TaqI TCGA 4 cut(s) 225, 467, 664, 710
TasI AATT 9 cut(s) 90, 175, 412, 483, 684, 891, 983, 1000, 1043
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 4 cut(s) 222, 494, 661, 954
Tru1I TTAA 4 cut(s) 683, 750, 804, 894
Tru9I TTAA 4 cut(s) 683, 750, 804, 894
TscAI CASTG 1 cut(s) 248
TseI GCWGC 2 cut(s) 716, 737
TspDTI ATGAA 9 cut(s) 371, 451, 545, 630, 770, 954, 1039, 1078, 1200
TspGWI ACGGA 1 cut(s) 1145
TspRI CASTG 1 cut(s) 248
Van91I CCANNNNNTGG 2 cut(s) 1015, 1158
Vha464I CTTAAG 1 cut(s) 803
VpaK11BI GGWCC 2 cut(s) 837, 904
XceI RCATGY 1 cut(s) 1015
XhoI CTCGAG 1 cut(s) 709
XmiI GTMKAC 1 cut(s) 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.