RchiOBHm_Chr1g0359721

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0009759
Physical Location & Seq
Reverse (-)
51591590 .. 51592481
892 bp
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UTR
Exon/CDS
Intron
PRQ58475

Sequence Viewer

Length: 186 bp
ATGATTACACAGTTGTTAACTGTTTTCCACTGCTTTGGTCAGTACTTCTGTCTCCATTTTGAATCATTCCAGCTCGGAATGGCTCCTGTTTACATGGCATTTCTACGTTTCGTGGGGGATGAAACAGAAGCTCGGAACTACACCTACAGCCTCGAGGTTGGAGGAAATGGCCGAAAGATCATATAG

Protein Analysis

61

Amino Acids

7.06

Weight (kDa)

6.0

Isoelectric Point (pI)

29.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sina_TRAF PF03145 9 - 60 7.4e-17 Sina, TRAF-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 152
AcoI YGGCCR 1 cut(s) 169
AfaI GTAC 1 cut(s) 44
AgsI TTSAA 1 cut(s) 62
AluBI AGCT 2 cut(s) 73, 131
AluI AGCT 2 cut(s) 73, 131
Alw26I GTCTC 1 cut(s) 56
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 1 cut(s) 169
AvaI CYCGRG 1 cut(s) 152
BcoDI GTCTC 1 cut(s) 56
BfmI CTRYAG 1 cut(s) 145
BmcAI AGTACT 1 cut(s) 44
BmeT110I CYCGRG 1 cut(s) 152
BmiI GGNNCC 1 cut(s) 84
BseGI GGATG 1 cut(s) 124
BshFI GGCC 1 cut(s) 171
BsiHKCI CYCGRG 1 cut(s) 152
BsmAI GTCTC 1 cut(s) 56
BsnI GGCC 1 cut(s) 171
BsoBI CYCGRG 1 cut(s) 152
Bsp143I GATC 1 cut(s) 177
BspANI GGCC 1 cut(s) 171
BspLI GGNNCC 1 cut(s) 84
BssMI GATC 1 cut(s) 177
Bst4CI ACNGT 2 cut(s) 12, 22
BstF5I GGATG 1 cut(s) 124
BstKTI GATC 1 cut(s) 180
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 1 cut(s) 177
BstSFI CTRYAG 1 cut(s) 145
BstXI CCANNNNNNTGG 1 cut(s) 35
BsuRI GGCC 1 cut(s) 171
BtsCI GGATG 1 cut(s) 124
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 1 cut(s) 28
Csp6I GTAC 1 cut(s) 43
CviAII CATG 1 cut(s) 94
CviJI RGCY 5 cut(s) 73, 83, 131, 150, 171
CviKI_1 RGCY 5 cut(s) 73, 83, 131, 150, 171
CviQI GTAC 1 cut(s) 43
DpnI GATC 1 cut(s) 179
DpnII GATC 1 cut(s) 177
EaeI YGGCCR 1 cut(s) 169
Eco88I CYCGRG 1 cut(s) 152
FaeI CATG 1 cut(s) 97
FaiI YATR 3 cut(s) 95, 182, 184
FatI CATG 1 cut(s) 93
FokI GGATG 1 cut(s) 131
HaeIII GGCC 1 cut(s) 171
Hin1II CATG 1 cut(s) 97
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HinfI GANTC 1 cut(s) 62
HpaI GTTAAC 1 cut(s) 18
Hpy166II GTNNAC 2 cut(s) 18, 91
Hpy188I TCNGA 2 cut(s) 77, 135
Hpy8I GTNNAC 2 cut(s) 18, 91
HpyCH4III ACNGT 2 cut(s) 12, 22
HpyCH4IV ACGT 1 cut(s) 106
HpySE526I ACGT 1 cut(s) 106
Hsp92II CATG 1 cut(s) 97
KspAI GTTAAC 1 cut(s) 18
Kzo9I GATC 1 cut(s) 177
LmnI GCTCC 1 cut(s) 88
LpnPI CCDG 2 cut(s) 83, 99
MaeII ACGT 1 cut(s) 106
MalI GATC 1 cut(s) 179
MboI GATC 1 cut(s) 177
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 3 cut(s) 148, 155, 161
MseI TTAA 1 cut(s) 17
NdeII GATC 1 cut(s) 177
NlaIII CATG 1 cut(s) 97
NlaIV GGNNCC 1 cut(s) 84
PaeR7I CTCGAG 1 cut(s) 152
PfeI GAWTC 1 cut(s) 62
PspN4I GGNNCC 1 cut(s) 84
PspXI VCTCGAGB 1 cut(s) 152
PsrI GAACNNNNNNTAC 2 cut(s) 128, 160
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
SaqAI TTAA 1 cut(s) 17
Sau3AI GATC 1 cut(s) 177
ScaI AGTACT 1 cut(s) 44
SetI ASST 5 cut(s) 75, 109, 133, 146, 159
SfcI CTRYAG 1 cut(s) 145
Sfr274I CTCGAG 1 cut(s) 152
SgeI CNNG 8 cut(s) 82, 86, 98, 106, 124, 144, 164, 166
SlaI CTCGAG 1 cut(s) 152
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
TaaI ACNGT 2 cut(s) 12, 22
TaiI ACGT 1 cut(s) 109
TaqI TCGA 1 cut(s) 153
TatI WGTACW 1 cut(s) 42
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TscAI CASTG 1 cut(s) 35
TspDTI ATGAA 1 cut(s) 135
TspRI CASTG 1 cut(s) 35
XhoI CTCGAG 1 cut(s) 152
ZrmI AGTACT 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.