RchiOBHm_Chr2g0134091

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008149
Physical Location & Seq
Forward (+)
51079008 .. 51081758
2751 bp
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UTR
Exon/CDS
Intron
PRQ50517

Sequence Viewer

Length: 1566 bp
ATGATGTGGTTTCTCATCTTCATCCTCTCCCTCTTCATCGCTTTCCTCCTCAAATCTCTTCTCTTTCTCTCGAAACCCTCGGCCAAACTCGACGGTACTATTCTACCGCCAGGACCCACCTCGATTCCCATAATCGGAAACTTCATATGGCTCTTCAAACTGACCTCCAGAATCGAACCCATCCTTCTTGACCTCCACGCCAAGTACGGCCCCATCGTCTCTCTCCCCATCCCCTTCAGCTCTCACCCCGCCATTTTCATCAACGACCACTCACTCGCCCACCAAGTCTTGGTCCAAAACGGAGCAGTCTGTTCGGACCGCCCTCCTCCCCAGCCCATCACCAAGCTCATCACTAGTGACAACCGTGTCATCTTCAGCAGTGGCTACGGCCCAACATGGCGTCTCCTCCGCCGAAACCTCATGTCCGAGGTTCTGAACCCTTCTCGGCTCAAGTCCTACGCTTCCTCCCGCAAATGGGCCTTGGACATCCTCACCAACCGTCTCGATCAGTCCGTATGGTCCAACGGACCAGTCGTCGAAGTTTTAGACCATTTTCGATTCTCCATAACTTCTTTGTTCGTGGATATGGTGTTTGGTGAGAAGGTGGAGGATGAGGCGAAGATGAAAGAAATTGCTGATGTGCAGCGTCGCTTTATGTTCGGTTTGGTCAGAGGTTTCCAGACCCTAAACTTGGGACCGAGATGGTTGAACAAGATTTTGTTCCGTAAGGGTTGGAATCAGTTCAACGAAATTCGACAGCAACAAAAGCACCTCCTGCTTCCATTCATACGGGCACGAAAGAAGGTGGTGGTGAAACAAGAAATGGTAGATAAGAACAGCGATGAGTTCACAATAGCATATGTTGATACATTAATGGATTTAGAAGACCCCCGCGAGAAGAGAGCCTTCAATGAAGAAGAACTAATGCATCAATGCTCGGAGATTTTGAATTCCGGCACGGACACCATGTCCATAACATTGCAGTGGACCATGGCGAATGTAGTGAAATACCCACGTGTTCAGAAGAGGCTTGTTGAAGATATTAAAAAGGTTGTGGGTGTTGAAAAATTAGAGCTGAAAGATGAGGATCTTCAGAAGATGCACTACCTAAAAGCGGTGGTGATGGAGGCTCTAAGGCTTCACCCACCAGGCCACTACGTGGTGCCACACACGGTTACAGAGGACATTATTCTGGCTGGACGTTATACGCTGCCAAAGAACTGTACTGTGAATTTTATGGTGGCCGAAATTGGTCGAGACTCGAGTGTGTGGGACGAGCCCATGGAGTTTAAACCTGAAAGGTTTATGAAAGATGGAGGTAACGTACAATGTGATATAACAGGGAGCAAGGAAATTATGATGATACCGTTTGGGGCGGGTAGGAGGATTTGTCCGGGATTGGGTTTGTCAATGCTTCTTTTGGAGTATTTGGTGGCAAATCTGGTTTGGAAGTACGAGTGGGAGGCTGTGGATGGAGTTGGTGTTGATTTGACTGAGAGACAAGAGGCCACCTGGGTAATGAAGAATCCACTACAGGCTCAAATATCCCCAAGGTTGAACAAATGA

Protein Analysis

521

Amino Acids

59.81

Weight (kDa)

8.73

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 36 - 511 4.4e-85 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 365
AccB1I GGYRCC 1 cut(s) 1162
AccB7I CCANNNNNTGG 2 cut(s) 289, 1159
AccII CGCG 1 cut(s) 894
AciI CCGC 8 cut(s) 107, 249, 319, 409, 469, 892, 1115, 1376
AclWI GGATC 1 cut(s) 1095
AcoI YGGCCR 2 cut(s) 81, 1242
AcsI RAATTY 3 cut(s) 750, 949, 1231
AcuI CTGAAG 3 cut(s) 220, 358, 1076
AcvI CACGTG 1 cut(s) 1016
AcyI GRCGYC 1 cut(s) 400
AdeI CACNNNGTG 1 cut(s) 1159
AfaI GTAC 5 cut(s) 97, 206, 1225, 1326, 1454
AfiI CCNNNNNNNGG 8 cut(s) 134, 289, 474, 475, 691, 1114, 1159, 1400
AflIII ACRYGT 1 cut(s) 1015
AgsI TTSAA 8 cut(s) 157, 709, 745, 910, 949, 1037, 1064, 1558
AhdI GACNNNNNGTC 2 cut(s) 533, 967
AhlI ACTAGT 1 cut(s) 353
AjnI CCWGG 3 cut(s) 109, 1147, 1511
AluBI AGCT 3 cut(s) 240, 346, 1075
AluI AGCT 3 cut(s) 240, 346, 1075
Alw26I GTCTC 5 cut(s) 223, 407, 506, 1251, 1492
AlwI GGATC 1 cut(s) 1095
Ama87I CYCGRG 1 cut(s) 1261
AoxI GGCC 7 cut(s) 81, 208, 388, 477, 1150, 1242, 1506
ApeKI GCWGC 2 cut(s) 643, 1210
ApoI RAATTY 3 cut(s) 750, 949, 1231
AseI ATTAAT 1 cut(s) 872
Asp700I GAANNNNTTC 1 cut(s) 740
AsuC2I CCSGG 1 cut(s) 1395
AsuHPI GGTGA 7 cut(s) 236, 331, 484, 608, 823, 1132, 1133
AvaI CYCGRG 1 cut(s) 1261
AvaII GGWCC 7 cut(s) 113, 292, 316, 519, 527, 695, 987
BaeGI GKGCMC 1 cut(s) 796
BanI GGYRCC 1 cut(s) 1162
BanII GRGCYC 1 cut(s) 1281
BarI GAAGNNNNNNTAC 2 cut(s) 1088, 1120
BbrPI CACGTG 1 cut(s) 1016
BbsI GAAGAC 1 cut(s) 891
BbvI GCAGC 2 cut(s) 655, 1197
BccI CCATC 8 cut(s) 188, 221, 236, 344, 696, 1117, 1307, 1466
BceAI ACGGC 2 cut(s) 223, 403
BcgI CGANNNNNNTGC 2 cut(s) 294, 328
BciT130I CCWGG 3 cut(s) 111, 1149, 1513
BcnI CCSGG 1 cut(s) 1395
BcoDI GTCTC 5 cut(s) 223, 407, 506, 1251, 1492
BcuI ACTAGT 1 cut(s) 353
BfaI CTAG 1 cut(s) 354
BfmI CTRYAG 1 cut(s) 1532
BisI GCNGC 2 cut(s) 644, 1211
BlsI GCNGC 2 cut(s) 645, 1212
Bme1390I CCNGG 4 cut(s) 111, 1149, 1395, 1513
Bme18I GGWCC 7 cut(s) 113, 292, 316, 519, 527, 695, 987
BmeRI GACNNNNNGTC 2 cut(s) 533, 967
BmeT110I CYCGRG 1 cut(s) 1261
BmiI GGNNCC 4 cut(s) 115, 211, 696, 1164
BmrFI CCNGG 4 cut(s) 111, 1149, 1395, 1513
BmsI GCATC 2 cut(s) 937, 1089
BpiI GAAGAC 1 cut(s) 891
BpmI CTGGAG 1 cut(s) 151
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 1 cut(s) 1395
BsaAI YACGTR 2 cut(s) 1016, 1159
BsaBI GATNNNNATC 1 cut(s) 1086
BsaHI GRCGYC 1 cut(s) 400
BsaJI CCNNGG 7 cut(s) 78, 426, 480, 990, 1281, 1512, 1550
BsaXI ACNNNNNCTCC 8 cut(s) 449, 479, 1308, 1338, 1375, 1405, 1467, 1497
Bsc4I CCNNNNNNNGG 8 cut(s) 134, 289, 474, 475, 691, 1114, 1159, 1400
Bse1I ACTGG 1 cut(s) 530
Bse3DI GCAATG 1 cut(s) 977
Bse8I GATNNNNATC 1 cut(s) 1086
BseBI CCWGG 3 cut(s) 111, 1149, 1513
BseDI CCNNGG 7 cut(s) 78, 426, 480, 990, 1281, 1512, 1550
BseGI GGATG 6 cut(s) 21, 180, 228, 486, 616, 1477
BseJI GATNNNNATC 1 cut(s) 1086
BseLI CCNNNNNNNGG 8 cut(s) 134, 289, 474, 475, 691, 1114, 1159, 1400
BseMI GCAATG 1 cut(s) 977
BseMII CTCAG 1 cut(s) 1485
BseNI ACTGG 1 cut(s) 530
BseRI GAGGAG 3 cut(s) 38, 315, 395
BseSI GKGCMC 1 cut(s) 796
BseXI GCAGC 2 cut(s) 655, 1197
BseYI CCCAGC 1 cut(s) 330
BsgI GTGCAG 1 cut(s) 662
Bsh1236I CGCG 1 cut(s) 894
BshFI GGCC 7 cut(s) 83, 210, 390, 479, 1152, 1244, 1508
BshNI GGYRCC 1 cut(s) 1162
BsiHKCI CYCGRG 1 cut(s) 1261
BsiSI CCGG 2 cut(s) 954, 1394
BslFI GGGAC 2 cut(s) 708, 1286
BslI CCNNNNNNNGG 8 cut(s) 134, 289, 474, 475, 691, 1114, 1159, 1400
BsmAI GTCTC 5 cut(s) 223, 407, 506, 1251, 1492
BsmBI CGTCTC 3 cut(s) 223, 407, 506
BsmFI GGGAC 2 cut(s) 708, 1286
BsnI GGCC 7 cut(s) 83, 210, 390, 479, 1152, 1244, 1508
BsoBI CYCGRG 1 cut(s) 1261
Bsp1286I GDGCHC 2 cut(s) 796, 1281
Bsp143I GATC 2 cut(s) 505, 1087
Bsp19I CCATGG 2 cut(s) 990, 1281
BspACI CCGC 8 cut(s) 107, 249, 319, 409, 469, 892, 1115, 1376
BspANI GGCC 7 cut(s) 83, 210, 390, 479, 1152, 1244, 1508
BspCNI CTCAG 1 cut(s) 1486
BspFNI CGCG 1 cut(s) 894
BspLI GGNNCC 4 cut(s) 115, 211, 696, 1164
BspPI GGATC 1 cut(s) 1095
BspQI GCTCTTC 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 1162
BsrDI GCAATG 1 cut(s) 977
BsrI ACTGG 1 cut(s) 530
BssECI CCNNGG 7 cut(s) 78, 426, 480, 990, 1281, 1512, 1550
BssMI GATC 2 cut(s) 505, 1087
BssNI GRCGYC 1 cut(s) 400
BssT1I CCWWGG 4 cut(s) 480, 990, 1281, 1550
Bst2UI CCWGG 3 cut(s) 111, 1149, 1513
Bst4CI ACNGT 7 cut(s) 95, 365, 500, 1174, 1223, 1228, 1368
Bst6I CTCTTC 5 cut(s) 38, 63, 158, 893, 1019
BstACI GRCGYC 1 cut(s) 400
BstAPI GCANNNNNTGC 1 cut(s) 775
BstBAI YACGTR 2 cut(s) 1016, 1159
BstDEI CTNAG 2 cut(s) 1133, 1494
BstDSI CCRYGG 2 cut(s) 990, 1281
BstF5I GGATG 6 cut(s) 21, 180, 228, 486, 616, 1477
BstFNI CGCG 1 cut(s) 894
BstKTI GATC 2 cut(s) 508, 1090
BstMAI GTCTC 5 cut(s) 223, 407, 506, 1251, 1492
BstMBI GATC 2 cut(s) 505, 1087
BstMWI GCNNNNNNNGC 2 cut(s) 766, 775
BstNI CCWGG 3 cut(s) 111, 1149, 1513
BstSCI CCNGG 4 cut(s) 109, 1147, 1393, 1511
BstSFI CTRYAG 1 cut(s) 1532
BstSLI GKGCMC 1 cut(s) 796
BstUI CGCG 1 cut(s) 894
BstV1I GCAGC 2 cut(s) 655, 1197
BstV2I GAAGAC 1 cut(s) 891
BstX2I RGATCY 1 cut(s) 1087
BstYI RGATCY 1 cut(s) 1087
BsuRI GGCC 7 cut(s) 83, 210, 390, 479, 1152, 1244, 1508
BtgI CCRYGG 2 cut(s) 990, 1281
BtgZI GCGATG 2 cut(s) 22, 855
BtsCI GGATG 6 cut(s) 21, 180, 228, 486, 616, 1477
BtsI GCAGTG 2 cut(s) 385, 989
BtsIMutI CAGTG 2 cut(s) 385, 989
CpoI CGGWCCG 1 cut(s) 316
CseI GACGC 2 cut(s) 389, 635
Csp6I GTAC 5 cut(s) 96, 205, 1224, 1325, 1453
CspI CGGWCCG 1 cut(s) 316
CviAII CATG 5 cut(s) 396, 421, 967, 991, 1282
CviQI GTAC 5 cut(s) 96, 205, 1224, 1325, 1453
DdeI CTNAG 2 cut(s) 1133, 1494
DpnI GATC 2 cut(s) 507, 1089
DpnII GATC 2 cut(s) 505, 1087
DraI TTTAAA 1 cut(s) 1291
DraIII CACNNNGTG 1 cut(s) 1159
DrdI GACNNNNNNGTC 1 cut(s) 365
DriI GACNNNNNGTC 2 cut(s) 533, 967
DseDI GACNNNNNNGTC 1 cut(s) 365
EaeI YGGCCR 2 cut(s) 81, 1242
Eam1104I CTCTTC 5 cut(s) 38, 63, 158, 893, 1019
Eam1105I GACNNNNNGTC 2 cut(s) 533, 967
EarI CTCTTC 5 cut(s) 38, 63, 158, 893, 1019
EciI GGCGGA 1 cut(s) 398
Eco130I CCWWGG 4 cut(s) 480, 990, 1281, 1550
Eco24I GRGCYC 1 cut(s) 1281
Eco47I GGWCC 7 cut(s) 113, 292, 316, 519, 527, 695, 987
Eco57I CTGAAG 3 cut(s) 220, 358, 1076
Eco72I CACGTG 1 cut(s) 1016
Eco88I CYCGRG 1 cut(s) 1261
EcoO109I RGGNCCY 1 cut(s) 113
EcoRI GAATTC 1 cut(s) 949
EcoRII CCWGG 3 cut(s) 109, 1147, 1511
EcoT14I CCWWGG 4 cut(s) 480, 990, 1281, 1550
EcoT22I ATGCAT 1 cut(s) 930
EcoT38I GRGCYC 1 cut(s) 1281
ErhI CCWWGG 4 cut(s) 480, 990, 1281, 1550
Esp3I CGTCTC 3 cut(s) 223, 407, 506
FaeI CATG 5 cut(s) 399, 424, 970, 994, 1285
FalI AAGNNNNNCTT 2 cut(s) 890, 922
FaqI GGGAC 2 cut(s) 708, 1286
FatI CATG 5 cut(s) 395, 420, 966, 990, 1281
FauI CCCGC 4 cut(s) 256, 476, 899, 1369
FauNDI CATATG 2 cut(s) 146, 859
Fnu4HI GCNGC 2 cut(s) 644, 1211
FokI GGATG 6 cut(s) 8, 167, 215, 473, 623, 1484
FriOI GRGCYC 1 cut(s) 1281
Fsp4HI GCNGC 2 cut(s) 644, 1211
FspBI CTAG 1 cut(s) 354
GluI GCNGC 2 cut(s) 644, 1211
GsaI CCCAGC 1 cut(s) 334
GsuI CTGGAG 1 cut(s) 151
HaeIII GGCC 7 cut(s) 83, 210, 390, 479, 1152, 1244, 1508
HapII CCGG 2 cut(s) 954, 1394
HgaI GACGC 2 cut(s) 389, 635
Hin1I GRCGYC 1 cut(s) 400
Hin1II CATG 5 cut(s) 399, 424, 970, 994, 1285
HinfI GANTC 6 cut(s) 124, 171, 558, 736, 1259, 1525
HpaII CCGG 2 cut(s) 954, 1394
HphI GGTGA 7 cut(s) 236, 331, 484, 608, 823, 1132, 1133
Hpy166II GTNNAC 2 cut(s) 849, 987
Hpy188I TCNGA 8 cut(s) 137, 316, 427, 435, 671, 940, 1023, 1095
Hpy188III TCNNGA 6 cut(s) 70, 168, 188, 503, 679, 1256
Hpy8I GTNNAC 2 cut(s) 849, 987
Hpy99I CGWCG 3 cut(s) 95, 539, 651
HpyAV CCTTC 6 cut(s) 194, 244, 450, 595, 796, 916
HpyCH4III ACNGT 7 cut(s) 95, 365, 500, 1174, 1223, 1228, 1368
HpyCH4IV ACGT 4 cut(s) 1015, 1158, 1201, 1323
HpyCH4V TGCA 4 cut(s) 643, 928, 982, 1102
HpyF10VI GCNNNNNNNGC 2 cut(s) 766, 775
HpyF3I CTNAG 2 cut(s) 1133, 1494
HpySE526I ACGT 4 cut(s) 1015, 1158, 1201, 1323
Hsp92I GRCGYC 1 cut(s) 400
Hsp92II CATG 5 cut(s) 399, 424, 970, 994, 1285
Kzo9I GATC 2 cut(s) 505, 1087
LguI GCTCTTC 1 cut(s) 158
LmnI GCTCC 2 cut(s) 302, 1344
Lsp1109I GCAGC 2 cut(s) 655, 1197
LweI GCATC 2 cut(s) 937, 1089
MaeI CTAG 1 cut(s) 354
MaeII ACGT 4 cut(s) 1015, 1158, 1201, 1323
MaeIII GTNAC 3 cut(s) 356, 1174, 1319
MalI GATC 2 cut(s) 507, 1089
MboI GATC 2 cut(s) 505, 1087
MflI RGATCY 1 cut(s) 1087
MhlI GDGCHC 2 cut(s) 796, 1281
MluCI AATT 7 cut(s) 630, 750, 949, 1067, 1231, 1248, 1353
MlyI GAGTC 1 cut(s) 1253
MmeI TCCRAC 2 cut(s) 546, 713
Mph1103I ATGCAT 1 cut(s) 930
MroXI GAANNNNTTC 1 cut(s) 740
MseI TTAA 3 cut(s) 872, 1044, 1290
MslI CAYNNNNRTG 1 cut(s) 982
MspI CCGG 2 cut(s) 954, 1394
MspR9I CCNGG 4 cut(s) 111, 1149, 1395, 1513
MssI GTTTAAAC 1 cut(s) 1291
MvaI CCWGG 3 cut(s) 111, 1149, 1513
MvnI CGCG 1 cut(s) 894
MwoI GCNNNNNNNGC 2 cut(s) 766, 775
NciI CCSGG 1 cut(s) 1395
NcoI CCATGG 2 cut(s) 990, 1281
NdeI CATATG 2 cut(s) 146, 859
NdeII GATC 2 cut(s) 505, 1087
NlaIII CATG 5 cut(s) 399, 424, 970, 994, 1285
NlaIV GGNNCC 4 cut(s) 115, 211, 696, 1164
NmeAIII GCCGAG 2 cut(s) 59, 424
NmuCI GTSAC 1 cut(s) 356
NsiI ATGCAT 1 cut(s) 930
PaeR7I CTCGAG 1 cut(s) 1261
PciSI GCTCTTC 1 cut(s) 158
PcsI WCGNNNNNNNCGW 3 cut(s) 213, 510, 531
PdmI GAANNNNTTC 1 cut(s) 740
PfeI GAWTC 5 cut(s) 124, 171, 558, 736, 1525
PflMI CCANNNNNTGG 2 cut(s) 289, 1159
PfoI TCCNGGA 1 cut(s) 1393
PkrI GCNGC 2 cut(s) 645, 1212
PleI GAGTC 1 cut(s) 1253
PmaCI CACGTG 1 cut(s) 1016
PmeI GTTTAAAC 1 cut(s) 1291
PmlI CACGTG 1 cut(s) 1016
PpsI GAGTC 1 cut(s) 1253
Ppu21I YACGTR 2 cut(s) 1016, 1159
PpuMI RGGWCCY 1 cut(s) 113
PshBI ATTAAT 1 cut(s) 872
Psp5II RGGWCCY 1 cut(s) 113
Psp6I CCWGG 3 cut(s) 109, 1147, 1511
PspCI CACGTG 1 cut(s) 1016
PspFI CCCAGC 1 cut(s) 330
PspGI CCWGG 3 cut(s) 109, 1147, 1511
PspN4I GGNNCC 4 cut(s) 115, 211, 696, 1164
PspPPI RGGWCCY 1 cut(s) 113
PspXI VCTCGAGB 1 cut(s) 1261
PsuI RGATCY 1 cut(s) 1087
RsaI GTAC 5 cut(s) 97, 206, 1225, 1326, 1454
RsaNI GTAC 5 cut(s) 96, 205, 1224, 1325, 1453
RseI CAYNNNNRTG 1 cut(s) 982
Rsr2I CGGWCCG 1 cut(s) 316
RsrII CGGWCCG 1 cut(s) 316
SapI GCTCTTC 1 cut(s) 158
SaqAI TTAA 3 cut(s) 872, 1044, 1290
SatI GCNGC 2 cut(s) 644, 1211
Sau3AI GATC 2 cut(s) 505, 1087
SchI GAGTC 1 cut(s) 1253
ScrFI CCNGG 4 cut(s) 111, 1149, 1395, 1513
SduI GDGCHC 2 cut(s) 796, 1281
SfaNI GCATC 2 cut(s) 937, 1089
SfcI CTRYAG 1 cut(s) 1532
Sfr274I CTCGAG 1 cut(s) 1261
SinI GGWCC 7 cut(s) 113, 292, 316, 519, 527, 695, 987
SlaI CTCGAG 1 cut(s) 1261
SmiMI CAYNNNNRTG 1 cut(s) 982
SmlI CTYRAG 2 cut(s) 449, 1261
SmoI CTYRAG 2 cut(s) 449, 1261
SpeI ACTAGT 1 cut(s) 353
Sse9I AATT 7 cut(s) 630, 750, 949, 1067, 1231, 1248, 1353
SsiI CCGC 8 cut(s) 107, 249, 319, 409, 469, 892, 1115, 1376
SspMI CTAG 1 cut(s) 354
StyD4I CCNGG 4 cut(s) 109, 1147, 1393, 1511
StyI CCWWGG 4 cut(s) 480, 990, 1281, 1550
TaaI ACNGT 7 cut(s) 95, 365, 500, 1174, 1223, 1228, 1368
TaiI ACGT 4 cut(s) 1018, 1161, 1204, 1326
TaqII GACCGA 1 cut(s) 712
TasI AATT 7 cut(s) 630, 750, 949, 1067, 1231, 1248, 1353
TatI WGTACW 1 cut(s) 1223
TfiI GAWTC 5 cut(s) 124, 171, 558, 736, 1525
Tru1I TTAA 3 cut(s) 872, 1044, 1290
Tru9I TTAA 3 cut(s) 872, 1044, 1290
TscAI CASTG 2 cut(s) 385, 989
TseFI GTSAC 1 cut(s) 356
TseI GCWGC 2 cut(s) 643, 1210
Tsp45I GTSAC 1 cut(s) 356
TspDTI ATGAA 9 cut(s) 10, 25, 133, 247, 638, 775, 927, 1322, 1535
TspGWI ACGGA 5 cut(s) 315, 502, 540, 713, 974
TspRI CASTG 2 cut(s) 385, 989
Van91I CCANNNNNTGG 2 cut(s) 289, 1159
VpaK11BI GGWCC 7 cut(s) 113, 292, 316, 519, 527, 695, 987
VspI ATTAAT 1 cut(s) 872
XapI RAATTY 3 cut(s) 750, 949, 1231
XhoI CTCGAG 1 cut(s) 1261
XmnI GAANNNNTTC 1 cut(s) 740
XspI CTAG 1 cut(s) 354
Zsp2I ATGCAT 1 cut(s) 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.