Rh2CG351400

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0008149
Physical Location & Seq
Forward (+)
47442295 .. 47445986
3692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG351400.1

Sequence Viewer

Length: 1572 bp
ATGGAAATGATGTGGTTTCTCATCTTCATCCTCTCCCTCTTCATCGCTTTCCTCCTCAAATCTCTTCTCTTTCTCTCGAAACCCTCGGCCAAACTCGACGGTACTATTCTACCGCCAGGACCCACCTCGATTCCCATAATCGGAAACTTCATATGGCTCTTCAAACTGACCTCCAGAATCGAACCCATCCTTCTTGACCTCCACGCCAAGTACGGCCCCATCGTCTCTCTCCCCATCCCCTTCAGCTCTCACCCCGCCATTTTCATCAACGACCACTCACTCGCCCACCAAGTCTTGGTCCAAAACGGAGCAGTCTGTTCGGACCGCCCTCCTCCCCAGCCCATCACCAAGCTCATCACTAGCGACAACCGTGTCATCTTCAGCAGTGGCTACGGCCCAACATGGCGTCTCCTTCGCCGAAACCTCATGTCCGGGGTTCTGAACCCTTCTCGGCTCAAGTCCTACGCTTCCTCCCGCAAATGGGCCTTGGACATCCTCACCAACCGTCTCGATCAGTCCGTATGGTCCAACGGACCAGTCGTCGAAGTTTTAGACCATTTTCGATTCTCCATAACTTCTTTGTTCGTGGATATGGTGTTTGGTGAGAAGGTGGAGGATGAGGCGAAGATGAAAGAAATTGCTGATGTGCAGCGTCGCTTTATGTTCGGTTTGGTCAGAGGTTTCCAGACCCTAAACTTGGGACCGAGATGGTTGAACAAGATTTTGTTCCGTAAGGGTTGGAATCAGTTCAACGAAATTCGACAGCAACAAAAGCACCTCCTGCTTCCATTCATACGGGCACGAAAGAAGGTGGTGGTGAAACAAGAAATGGTAGATAAGAACAGCGATGAGTTCACAATAGCATATGTTGATACATTAATGGATTTAGAAGACCCCCGCGAGAAGAGAGCCTTCAATGAAGAAGAACTAATGCATCAATGCTCGGAGATTTTGAACTCCGGCACGGACACCATGTCCATAACATTGCAGTGGACGATGGCGAACGTAGTGAAATACCCACGTGTTCAGAAGAGGCTTGTTGAAGATATTAAAAAGGTTGTGGGTGTTGAAAAAATAGAGCTGAAAGATGAGGATCTTCAGAATATGCACTACCTAAAAGCGGTGGTGATGGAGGCTCTAAGGCTTCACCCACCAGGCCACTATGTGGTGCCACACACGGTTACAGAGGACATTGTTCTGGCTGGACGTTATACGCTGCCAAAGAACTGTACTGTGAATTTTATGGTGGCCGAAATTGGTCGAGACTCGAGTGTGTGGGACGAGCCCATGGAGTTTAAACCTGAAAGGTTTATGAAAGATGGAGGTAACGTACAATGTGATATAATGGGGAGCAAGGAAATTATGATGATACCGTTTGGGGCGGGTAGGAGGATTTGTCCGGGATTGGGTTTGTCAATGCTTCTTTTGGAGTATTTGGTGGCAAATCTGGTTTGGAAGTACGAGTGGGAAGCTGTGGATGGAGTTGGTGTTGATTTGACTGAGAGACAAGAGGCCACCTGGGTAATGAAGAATCCACTACAGGCTCATATATCCCCAAGGTTGAACAAATGA

Protein Analysis

523

Amino Acids

60.01

Weight (kDa)

8.6

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 38 - 510 5.8e-85 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 371
AccB1I GGYRCC 1 cut(s) 1168
AccB7I CCANNNNNTGG 2 cut(s) 295, 1165
AccII CGCG 1 cut(s) 900
AciI CCGC 7 cut(s) 113, 255, 325, 475, 898, 1121, 1382
AclWI GGATC 1 cut(s) 1101
AcoI YGGCCR 2 cut(s) 87, 1248
AcsI RAATTY 2 cut(s) 756, 1237
AcuI CTGAAG 3 cut(s) 226, 364, 1082
AcvI CACGTG 1 cut(s) 1022
AcyI GRCGYC 1 cut(s) 406
AdeI CACNNNGTG 1 cut(s) 1165
AfaI GTAC 5 cut(s) 103, 212, 1231, 1332, 1460
AfiI CCNNNNNNNGG 8 cut(s) 140, 295, 480, 481, 697, 1120, 1165, 1406
AflIII ACRYGT 1 cut(s) 1021
AgsI TTSAA 8 cut(s) 163, 715, 751, 916, 955, 1043, 1070, 1564
AhdI GACNNNNNGTC 2 cut(s) 539, 973
AjnI CCWGG 3 cut(s) 115, 1153, 1517
AluBI AGCT 4 cut(s) 246, 352, 1081, 1472
AluI AGCT 4 cut(s) 246, 352, 1081, 1472
Alw26I GTCTC 5 cut(s) 229, 413, 512, 1257, 1498
AlwI GGATC 1 cut(s) 1101
Ama87I CYCGRG 1 cut(s) 1267
AoxI GGCC 7 cut(s) 87, 214, 394, 483, 1156, 1248, 1512
ApeKI GCWGC 2 cut(s) 649, 1216
ApoI RAATTY 2 cut(s) 756, 1237
AseI ATTAAT 1 cut(s) 878
Asp700I GAANNNNTTC 1 cut(s) 746
AspS9I GGNCC 9 cut(s) 119, 215, 298, 322, 395, 483, 525, 533, 701
AsuC2I CCSGG 2 cut(s) 433, 1401
AsuHPI GGTGA 7 cut(s) 242, 337, 490, 614, 829, 1138, 1139
AvaI CYCGRG 1 cut(s) 1267
AvaII GGWCC 6 cut(s) 119, 298, 322, 525, 533, 701
BaeGI GKGCMC 1 cut(s) 802
BanI GGYRCC 1 cut(s) 1168
BanII GRGCYC 1 cut(s) 1287
BbrPI CACGTG 1 cut(s) 1022
BbsI GAAGAC 1 cut(s) 897
BbvI GCAGC 2 cut(s) 661, 1203
BccI CCATC 9 cut(s) 194, 227, 242, 350, 702, 991, 1123, 1313, 1472
BceAI ACGGC 2 cut(s) 229, 409
BcgI CGANNNNNNTGC 2 cut(s) 300, 334
BciT130I CCWGG 3 cut(s) 117, 1155, 1519
BcnI CCSGG 2 cut(s) 433, 1401
BcoDI GTCTC 5 cut(s) 229, 413, 512, 1257, 1498
BfaI CTAG 1 cut(s) 360
BfmI CTRYAG 1 cut(s) 1538
BisI GCNGC 2 cut(s) 650, 1217
BlsI GCNGC 2 cut(s) 651, 1218
Bme1390I CCNGG 5 cut(s) 117, 433, 1155, 1401, 1519
Bme18I GGWCC 6 cut(s) 119, 298, 322, 525, 533, 701
BmeRI GACNNNNNGTC 2 cut(s) 539, 973
BmeT110I CYCGRG 1 cut(s) 1267
BmgT120I GGNCC 9 cut(s) 119, 215, 298, 322, 395, 483, 525, 533, 701
BmiI GGNNCC 4 cut(s) 121, 217, 702, 1170
BmrFI CCNGG 5 cut(s) 117, 433, 1155, 1401, 1519
BmsI GCATC 1 cut(s) 943
BpiI GAAGAC 1 cut(s) 897
BpmI CTGGAG 1 cut(s) 157
BpuEI CTTGAG 1 cut(s) 440
BpuMI CCSGG 2 cut(s) 433, 1401
BsaAI YACGTR 1 cut(s) 1022
BsaBI GATNNNNATC 1 cut(s) 1092
BsaHI GRCGYC 1 cut(s) 406
BsaJI CCNNGG 6 cut(s) 84, 432, 486, 1287, 1518, 1556
BsaXI ACNNNNNCTCC 8 cut(s) 455, 485, 1314, 1344, 1381, 1411, 1473, 1503
Bsc4I CCNNNNNNNGG 8 cut(s) 140, 295, 480, 481, 697, 1120, 1165, 1406
Bse1I ACTGG 1 cut(s) 536
Bse3DI GCAATG 1 cut(s) 983
Bse8I GATNNNNATC 1 cut(s) 1092
BseBI CCWGG 3 cut(s) 117, 1155, 1519
BseDI CCNNGG 6 cut(s) 84, 432, 486, 1287, 1518, 1556
BseGI GGATG 6 cut(s) 27, 186, 234, 492, 622, 1483
BseJI GATNNNNATC 1 cut(s) 1092
BseLI CCNNNNNNNGG 8 cut(s) 140, 295, 480, 481, 697, 1120, 1165, 1406
BseMI GCAATG 1 cut(s) 983
BseMII CTCAG 1 cut(s) 1491
BseNI ACTGG 1 cut(s) 536
BseRI GAGGAG 2 cut(s) 44, 321
BseSI GKGCMC 1 cut(s) 802
BseXI GCAGC 2 cut(s) 661, 1203
BseYI CCCAGC 1 cut(s) 336
BsgI GTGCAG 1 cut(s) 668
Bsh1236I CGCG 1 cut(s) 900
BshFI GGCC 7 cut(s) 89, 216, 396, 485, 1158, 1250, 1514
BshNI GGYRCC 1 cut(s) 1168
BsiHKCI CYCGRG 1 cut(s) 1267
BsiSI CCGG 3 cut(s) 432, 960, 1400
BslFI GGGAC 2 cut(s) 714, 1292
BslI CCNNNNNNNGG 8 cut(s) 140, 295, 480, 481, 697, 1120, 1165, 1406
BsmAI GTCTC 5 cut(s) 229, 413, 512, 1257, 1498
BsmBI CGTCTC 3 cut(s) 229, 413, 512
BsmFI GGGAC 2 cut(s) 714, 1292
BsnI GGCC 7 cut(s) 89, 216, 396, 485, 1158, 1250, 1514
BsoBI CYCGRG 1 cut(s) 1267
Bsp1286I GDGCHC 2 cut(s) 802, 1287
Bsp143I GATC 2 cut(s) 511, 1093
Bsp19I CCATGG 1 cut(s) 1287
BspACI CCGC 7 cut(s) 113, 255, 325, 475, 898, 1121, 1382
BspANI GGCC 7 cut(s) 89, 216, 396, 485, 1158, 1250, 1514
BspCNI CTCAG 1 cut(s) 1492
BspFNI CGCG 1 cut(s) 900
BspLI GGNNCC 4 cut(s) 121, 217, 702, 1170
BspPI GGATC 1 cut(s) 1101
BspQI GCTCTTC 1 cut(s) 164
BspT107I GGYRCC 1 cut(s) 1168
BsrDI GCAATG 1 cut(s) 983
BsrI ACTGG 1 cut(s) 536
BssECI CCNNGG 6 cut(s) 84, 432, 486, 1287, 1518, 1556
BssMI GATC 2 cut(s) 511, 1093
BssNI GRCGYC 1 cut(s) 406
BssT1I CCWWGG 3 cut(s) 486, 1287, 1556
Bst2UI CCWGG 3 cut(s) 117, 1155, 1519
Bst4CI ACNGT 7 cut(s) 101, 371, 506, 1180, 1229, 1234, 1374
Bst6I CTCTTC 5 cut(s) 44, 69, 164, 899, 1025
BstACI GRCGYC 1 cut(s) 406
BstAPI GCANNNNNTGC 1 cut(s) 781
BstBAI YACGTR 1 cut(s) 1022
BstDEI CTNAG 2 cut(s) 1139, 1500
BstDSI CCRYGG 1 cut(s) 1287
BstF5I GGATG 6 cut(s) 27, 186, 234, 492, 622, 1483
BstFNI CGCG 1 cut(s) 900
BstKTI GATC 2 cut(s) 514, 1096
BstMAI GTCTC 5 cut(s) 229, 413, 512, 1257, 1498
BstMBI GATC 2 cut(s) 511, 1093
BstMWI GCNNNNNNNGC 2 cut(s) 772, 781
BstNI CCWGG 3 cut(s) 117, 1155, 1519
BstSCI CCNGG 5 cut(s) 115, 431, 1153, 1399, 1517
BstSFI CTRYAG 1 cut(s) 1538
BstSLI GKGCMC 1 cut(s) 802
BstUI CGCG 1 cut(s) 900
BstV1I GCAGC 2 cut(s) 661, 1203
BstV2I GAAGAC 1 cut(s) 897
BstX2I RGATCY 1 cut(s) 1093
BstYI RGATCY 1 cut(s) 1093
BsuRI GGCC 7 cut(s) 89, 216, 396, 485, 1158, 1250, 1514
BtgI CCRYGG 1 cut(s) 1287
BtgZI GCGATG 2 cut(s) 28, 861
BtsCI GGATG 6 cut(s) 27, 186, 234, 492, 622, 1483
BtsI GCAGTG 2 cut(s) 391, 995
BtsIMutI CAGTG 2 cut(s) 391, 995
Cfr13I GGNCC 9 cut(s) 119, 215, 298, 322, 395, 483, 525, 533, 701
CpoI CGGWCCG 1 cut(s) 322
CseI GACGC 2 cut(s) 395, 641
Csp6I GTAC 5 cut(s) 102, 211, 1230, 1331, 1459
CspI CGGWCCG 1 cut(s) 322
CviAII CATG 4 cut(s) 402, 427, 973, 1288
CviQI GTAC 5 cut(s) 102, 211, 1230, 1331, 1459
DdeI CTNAG 2 cut(s) 1139, 1500
DpnI GATC 2 cut(s) 513, 1095
DpnII GATC 2 cut(s) 511, 1093
DraI TTTAAA 1 cut(s) 1297
DraIII CACNNNGTG 1 cut(s) 1165
DrdI GACNNNNNNGTC 1 cut(s) 371
DriI GACNNNNNGTC 2 cut(s) 539, 973
DseDI GACNNNNNNGTC 1 cut(s) 371
EaeI YGGCCR 2 cut(s) 87, 1248
Eam1104I CTCTTC 5 cut(s) 44, 69, 164, 899, 1025
Eam1105I GACNNNNNGTC 2 cut(s) 539, 973
EarI CTCTTC 5 cut(s) 44, 69, 164, 899, 1025
Eco130I CCWWGG 3 cut(s) 486, 1287, 1556
Eco24I GRGCYC 1 cut(s) 1287
Eco47I GGWCC 6 cut(s) 119, 298, 322, 525, 533, 701
Eco57I CTGAAG 3 cut(s) 226, 364, 1082
Eco72I CACGTG 1 cut(s) 1022
Eco88I CYCGRG 1 cut(s) 1267
EcoO109I RGGNCCY 1 cut(s) 119
EcoRII CCWGG 3 cut(s) 115, 1153, 1517
EcoT14I CCWWGG 3 cut(s) 486, 1287, 1556
EcoT22I ATGCAT 1 cut(s) 936
EcoT38I GRGCYC 1 cut(s) 1287
ErhI CCWWGG 3 cut(s) 486, 1287, 1556
Esp3I CGTCTC 3 cut(s) 229, 413, 512
FaeI CATG 4 cut(s) 405, 430, 976, 1291
FalI AAGNNNNNCTT 2 cut(s) 896, 928
FaqI GGGAC 2 cut(s) 714, 1292
FatI CATG 4 cut(s) 401, 426, 972, 1287
FauI CCCGC 4 cut(s) 262, 482, 905, 1375
FauNDI CATATG 2 cut(s) 152, 865
Fnu4HI GCNGC 2 cut(s) 650, 1217
FokI GGATG 6 cut(s) 14, 173, 221, 479, 629, 1490
FriOI GRGCYC 1 cut(s) 1287
Fsp4HI GCNGC 2 cut(s) 650, 1217
FspBI CTAG 1 cut(s) 360
GluI GCNGC 2 cut(s) 650, 1217
GsaI CCCAGC 1 cut(s) 340
GsuI CTGGAG 1 cut(s) 157
HaeIII GGCC 7 cut(s) 89, 216, 396, 485, 1158, 1250, 1514
HapII CCGG 3 cut(s) 432, 960, 1400
HgaI GACGC 2 cut(s) 395, 641
Hin1I GRCGYC 1 cut(s) 406
Hin1II CATG 4 cut(s) 405, 430, 976, 1291
HinfI GANTC 6 cut(s) 130, 177, 564, 742, 1265, 1531
HpaII CCGG 3 cut(s) 432, 960, 1400
HphI GGTGA 7 cut(s) 242, 337, 490, 614, 829, 1138, 1139
Hpy166II GTNNAC 2 cut(s) 855, 993
Hpy188I TCNGA 7 cut(s) 143, 322, 441, 677, 946, 1029, 1101
Hpy188III TCNNGA 6 cut(s) 76, 174, 194, 509, 685, 1262
Hpy8I GTNNAC 2 cut(s) 855, 993
Hpy99I CGWCG 3 cut(s) 101, 545, 657
HpyAV CCTTC 7 cut(s) 200, 250, 422, 456, 601, 802, 922
HpyCH4III ACNGT 7 cut(s) 101, 371, 506, 1180, 1229, 1234, 1374
HpyCH4IV ACGT 4 cut(s) 1005, 1021, 1207, 1329
HpyCH4V TGCA 4 cut(s) 649, 934, 988, 1108
HpyF10VI GCNNNNNNNGC 2 cut(s) 772, 781
HpyF3I CTNAG 2 cut(s) 1139, 1500
HpySE526I ACGT 4 cut(s) 1005, 1021, 1207, 1329
Hsp92I GRCGYC 1 cut(s) 406
Hsp92II CATG 4 cut(s) 405, 430, 976, 1291
Kzo9I GATC 2 cut(s) 511, 1093
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 2 cut(s) 308, 1350
Lsp1109I GCAGC 2 cut(s) 661, 1203
LweI GCATC 1 cut(s) 943
MaeI CTAG 1 cut(s) 360
MaeII ACGT 4 cut(s) 1005, 1021, 1207, 1329
MaeIII GTNAC 2 cut(s) 1180, 1325
MalI GATC 2 cut(s) 513, 1095
MboI GATC 2 cut(s) 511, 1093
MflI RGATCY 1 cut(s) 1093
MhlI GDGCHC 2 cut(s) 802, 1287
MluCI AATT 5 cut(s) 636, 756, 1237, 1254, 1359
MlyI GAGTC 1 cut(s) 1259
MmeI TCCRAC 2 cut(s) 552, 719
Mph1103I ATGCAT 1 cut(s) 936
MroXI GAANNNNTTC 1 cut(s) 746
MseI TTAA 3 cut(s) 878, 1050, 1296
MslI CAYNNNNRTG 1 cut(s) 988
MspI CCGG 3 cut(s) 432, 960, 1400
MspR9I CCNGG 5 cut(s) 117, 433, 1155, 1401, 1519
MssI GTTTAAAC 1 cut(s) 1297
MvaI CCWGG 3 cut(s) 117, 1155, 1519
MvnI CGCG 1 cut(s) 900
MwoI GCNNNNNNNGC 2 cut(s) 772, 781
NciI CCSGG 2 cut(s) 433, 1401
NcoI CCATGG 1 cut(s) 1287
NdeI CATATG 2 cut(s) 152, 865
NdeII GATC 2 cut(s) 511, 1093
NlaIII CATG 4 cut(s) 405, 430, 976, 1291
NlaIV GGNNCC 4 cut(s) 121, 217, 702, 1170
NmeAIII GCCGAG 2 cut(s) 65, 430
NsiI ATGCAT 1 cut(s) 936
PaeR7I CTCGAG 1 cut(s) 1267
PciSI GCTCTTC 1 cut(s) 164
PcsI WCGNNNNNNNCGW 3 cut(s) 219, 516, 537
PdmI GAANNNNTTC 1 cut(s) 746
PfeI GAWTC 5 cut(s) 130, 177, 564, 742, 1531
PflMI CCANNNNNTGG 2 cut(s) 295, 1165
PfoI TCCNGGA 1 cut(s) 1399
PkrI GCNGC 2 cut(s) 651, 1218
PleI GAGTC 1 cut(s) 1259
PmaCI CACGTG 1 cut(s) 1022
PmeI GTTTAAAC 1 cut(s) 1297
PmlI CACGTG 1 cut(s) 1022
PpsI GAGTC 1 cut(s) 1259
Ppu21I YACGTR 1 cut(s) 1022
PpuMI RGGWCCY 1 cut(s) 119
PshBI ATTAAT 1 cut(s) 878
Psp5II RGGWCCY 1 cut(s) 119
Psp6I CCWGG 3 cut(s) 115, 1153, 1517
PspCI CACGTG 1 cut(s) 1022
PspFI CCCAGC 1 cut(s) 336
PspGI CCWGG 3 cut(s) 115, 1153, 1517
PspN4I GGNNCC 4 cut(s) 121, 217, 702, 1170
PspPI GGNCC 9 cut(s) 119, 215, 298, 322, 395, 483, 525, 533, 701
PspPPI RGGWCCY 1 cut(s) 119
PspXI VCTCGAGB 1 cut(s) 1267
PsuI RGATCY 1 cut(s) 1093
RsaI GTAC 5 cut(s) 103, 212, 1231, 1332, 1460
RsaNI GTAC 5 cut(s) 102, 211, 1230, 1331, 1459
RseI CAYNNNNRTG 1 cut(s) 988
Rsr2I CGGWCCG 1 cut(s) 322
RsrII CGGWCCG 1 cut(s) 322
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 3 cut(s) 878, 1050, 1296
SatI GCNGC 2 cut(s) 650, 1217
Sau3AI GATC 2 cut(s) 511, 1093
Sau96I GGNCC 9 cut(s) 119, 215, 298, 322, 395, 483, 525, 533, 701
SchI GAGTC 1 cut(s) 1259
ScrFI CCNGG 5 cut(s) 117, 433, 1155, 1401, 1519
SduI GDGCHC 2 cut(s) 802, 1287
SfaNI GCATC 1 cut(s) 943
SfcI CTRYAG 1 cut(s) 1538
Sfr274I CTCGAG 1 cut(s) 1267
SinI GGWCC 6 cut(s) 119, 298, 322, 525, 533, 701
SlaI CTCGAG 1 cut(s) 1267
SmiMI CAYNNNNRTG 1 cut(s) 988
SmlI CTYRAG 2 cut(s) 455, 1267
SmoI CTYRAG 2 cut(s) 455, 1267
Sse9I AATT 5 cut(s) 636, 756, 1237, 1254, 1359
SsiI CCGC 7 cut(s) 113, 255, 325, 475, 898, 1121, 1382
SspMI CTAG 1 cut(s) 360
StyD4I CCNGG 5 cut(s) 115, 431, 1153, 1399, 1517
StyI CCWWGG 3 cut(s) 486, 1287, 1556
TaaI ACNGT 7 cut(s) 101, 371, 506, 1180, 1229, 1234, 1374
TaiI ACGT 4 cut(s) 1008, 1024, 1210, 1332
TaqII GACCGA 1 cut(s) 718
TasI AATT 5 cut(s) 636, 756, 1237, 1254, 1359
TatI WGTACW 1 cut(s) 1229
TfiI GAWTC 5 cut(s) 130, 177, 564, 742, 1531
Tru1I TTAA 3 cut(s) 878, 1050, 1296
Tru9I TTAA 3 cut(s) 878, 1050, 1296
TscAI CASTG 2 cut(s) 391, 995
TseI GCWGC 2 cut(s) 649, 1216
TspDTI ATGAA 9 cut(s) 16, 31, 139, 253, 644, 781, 933, 1328, 1541
TspGWI ACGGA 5 cut(s) 321, 508, 546, 719, 980
TspRI CASTG 2 cut(s) 391, 995
Van91I CCANNNNNTGG 2 cut(s) 295, 1165
VpaK11BI GGWCC 6 cut(s) 119, 298, 322, 525, 533, 701
VspI ATTAAT 1 cut(s) 878
XapI RAATTY 2 cut(s) 756, 1237
XhoI CTCGAG 1 cut(s) 1267
XmnI GAANNNNTTC 1 cut(s) 746
XspI CTAG 1 cut(s) 360
Zsp2I ATGCAT 1 cut(s) 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.