RchiOBHm_Chr2g0134201

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008149
Physical Location & Seq
Forward (+)
51176699 .. 51181118
4420 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50525

Sequence Viewer

Length: 1185 bp
ATGTGCAGCTTAGCTTTGGTCAGAGGTTTCCAGACCCTAAACTTGGGACCGAGATGGTTGAACAAGATTTTGTTCCGTAAGGGTTGGAATCAGTTCAACGAAATTCGACAGCAACAAAAGCTCCTCCTGCTTCCTTTCATACGGGCACGAAAGAAGGTGATGGGGGTAACGATATCAGGAAGACCGAGGTCGGAGATGCAGTTGTTGACGACGACTGCAGTTGAGGTCAACGGCGGTGAGAGAGAGAGAGTTGGTGACGACGCCATGGTGGCCGGCCCGACTCTGGTAGGAGGTGGAGTTGGCGTCGGGGCTTTGCAGGCCGGGGAAGGCGGGGGAAGAGAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAACACAAATCGGAGACTGAGAGAGAGAGAGAAAGAGAGAGAGTAGACAGAATGGCAGTGGGTAATTTTTTAATTAGTTTGAGGGCAAAATGGTCATTTGAGCTTAAATTGTGTATCTGGGAACAAAAATCTGTTGCTGGGAGAGCCTTCAATGAAGAAGAACTAATGCATCAATGCTCGGAGATTTCGAACTCCGGAATGGACACCATGTCGATAACAATGCAGTGGACGATGGCGAATGTAGTGAAATACCCACGTCTTCAGAAGAGGCTTGTTGAAGATATTAAAAACGTTGTGGGGATTGAAAAAATAGAGCTGAAAGATGAGAATCTTCAGAAGATGCACTACCTAAAAGCGGTGGTGATGGAGGCTCTAAGGCTTCACCCACCAGGCCACTATGTGGACATTGTTCTGGATGGACGTTATACGCTGCCAAAGAACTGTACTGTGAATTTTATGGTGGCCGAAATTGGTCGAGACTCGAGTTTGTGGGACGAGCCCATGGAGTTTAAACCTGAAAGGTTTATGAAAGATGGAGGTAACGTACAATGTGATATAACAGGGAGCAAGGAGATTATGATGATACCGTTTGGGGCGGGTAGGAGAATTTGTCCGGGATTGGGTTTGTCAATGCTTCTTTTGGAGTATTTGGTGGCAAATCTGGTGTGGAAGTACGAGTGGGAAGCTGTGGATGGAGTTGGTGTTGATTTGACTGAGAGGCAAGAGGCCACCTGGGTAATGAAGAATCCACTACAGGCTCATATATCTCCAAGGTTGAACAAATGA

Protein Analysis

394

Amino Acids

45.29

Weight (kDa)

8.92

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 177 - 381 2e-48 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 799
AccI GTMKAC 1 cut(s) 444
AccIII TCCGGA 1 cut(s) 593
AciI CCGC 4 cut(s) 234, 330, 755, 995
AclI AACGTT 1 cut(s) 690
AcoI YGGCCR 2 cut(s) 270, 861
AcsI RAATTY 3 cut(s) 102, 850, 1005
AcuI CTGAAG 2 cut(s) 644, 716
AcyI GRCGYC 2 cut(s) 261, 303
AdeI CACNNNGTG 1 cut(s) 799
AfaI GTAC 3 cut(s) 844, 945, 1073
AfiI CCNNNNNNNGG 5 cut(s) 43, 283, 754, 799, 1019
AgsI TTSAA 6 cut(s) 61, 97, 550, 677, 704, 1177
AhdI GACNNNNNGTC 1 cut(s) 607
AjiI CACGTC 1 cut(s) 656
AjnI CCWGG 2 cut(s) 787, 1130
AluBI AGCT 6 cut(s) 9, 14, 121, 502, 715, 1085
AluI AGCT 6 cut(s) 9, 14, 121, 502, 715, 1085
Alw26I GTCTC 2 cut(s) 407, 870
Ama87I CYCGRG 1 cut(s) 880
Aor13HI TCCGGA 1 cut(s) 593
AoxI GGCC 6 cut(s) 270, 274, 318, 790, 861, 1125
ApeKI GCWGC 2 cut(s) 6, 829
ApoI RAATTY 3 cut(s) 102, 850, 1005
Asp700I GAANNNNTTC 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 47, 275
AsuC2I CCSGG 2 cut(s) 322, 1014
AsuHPI GGTGA 5 cut(s) 169, 248, 266, 772, 773
AsuII TTCGAA 1 cut(s) 587
AvaI CYCGRG 1 cut(s) 880
AvaII GGWCC 1 cut(s) 47
BaeGI GKGCMC 1 cut(s) 148
BanII GRGCYC 1 cut(s) 900
BarI GAAGNNNNNNTAC 2 cut(s) 728, 760
BbsI GAAGAC 2 cut(s) 187, 650
BbvI GCAGC 2 cut(s) 18, 816
BccI CCATC 7 cut(s) 48, 154, 625, 757, 809, 926, 1085
BceAI ACGGC 1 cut(s) 247
BcgI CGANNNNNNTGC 2 cut(s) 601, 635
BciT130I CCWGG 2 cut(s) 789, 1132
BcnI CCSGG 2 cut(s) 322, 1014
BcoDI GTCTC 2 cut(s) 407, 870
BfmI CTRYAG 2 cut(s) 216, 1151
BglI GCCNNNNNGGC 1 cut(s) 269
BisI GCNGC 2 cut(s) 7, 830
BlpI GCTNAGC 1 cut(s) 10
BlsI GCNGC 2 cut(s) 8, 831
Bme1390I CCNGG 4 cut(s) 322, 789, 1014, 1132
Bme18I GGWCC 1 cut(s) 47
BmeRI GACNNNNNGTC 1 cut(s) 607
BmeT110I CYCGRG 1 cut(s) 880
BmgBI CACGTC 1 cut(s) 656
BmgT120I GGNCC 2 cut(s) 47, 275
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 4 cut(s) 322, 789, 1014, 1132
BmsI GCATC 3 cut(s) 186, 577, 729
BoxI GACNNNNGTC 1 cut(s) 187
BpiI GAAGAC 2 cut(s) 187, 650
Bpu1102I GCTNAGC 1 cut(s) 10
Bpu14I TTCGAA 1 cut(s) 587
BpuMI CCSGG 2 cut(s) 322, 1014
BsaBI GATNNNNATC 1 cut(s) 726
BsaHI GRCGYC 2 cut(s) 261, 303
BsaJI CCNNGG 6 cut(s) 185, 264, 321, 900, 1131, 1169
BsaWI WCCGGW 1 cut(s) 593
Bsc4I CCNNNNNNNGG 5 cut(s) 43, 283, 754, 799, 1019
Bse118I RCCGGY 1 cut(s) 272
Bse8I GATNNNNATC 1 cut(s) 726
BseAI TCCGGA 1 cut(s) 593
BseBI CCWGG 2 cut(s) 789, 1132
BseDI CCNNGG 6 cut(s) 185, 264, 321, 900, 1131, 1169
BseGI GGATG 2 cut(s) 820, 1096
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 5 cut(s) 43, 283, 754, 799, 1019
BseMII CTCAG 2 cut(s) 408, 1104
BseRI GAGGAG 1 cut(s) 113
BseSI GKGCMC 1 cut(s) 148
BseXI GCAGC 2 cut(s) 18, 816
BseYI CCCAGC 1 cut(s) 536
BsgI GTGCAG 1 cut(s) 25
BshFI GGCC 6 cut(s) 272, 276, 320, 792, 863, 1127
BsiHKCI CYCGRG 1 cut(s) 880
BsiSI CCGG 4 cut(s) 273, 321, 594, 1013
BslFI GGGAC 2 cut(s) 60, 905
BslI CCNNNNNNNGG 5 cut(s) 43, 283, 754, 799, 1019
BsmAI GTCTC 2 cut(s) 407, 870
BsmFI GGGAC 2 cut(s) 60, 905
BsnI GGCC 6 cut(s) 272, 276, 320, 792, 863, 1127
BsoBI CYCGRG 1 cut(s) 880
Bsp119I TTCGAA 1 cut(s) 587
Bsp1286I GDGCHC 2 cut(s) 148, 900
Bsp13I TCCGGA 1 cut(s) 593
Bsp1720I GCTNAGC 1 cut(s) 10
Bsp19I CCATGG 2 cut(s) 264, 900
BspACI CCGC 4 cut(s) 234, 330, 755, 995
BspANI GGCC 6 cut(s) 272, 276, 320, 792, 863, 1127
BspCNI CTCAG 2 cut(s) 409, 1105
BspEI TCCGGA 1 cut(s) 593
BspLI GGNNCC 1 cut(s) 48
BspMAI CTGCAG 1 cut(s) 220
BspT104I TTCGAA 1 cut(s) 587
BsrFI RCCGGY 1 cut(s) 272
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 6 cut(s) 185, 264, 321, 900, 1131, 1169
BssNI GRCGYC 2 cut(s) 261, 303
BssT1I CCWWGG 3 cut(s) 264, 900, 1169
Bst2UI CCWGG 2 cut(s) 789, 1132
Bst4CI ACNGT 3 cut(s) 842, 847, 987
Bst6I CTCTTC 2 cut(s) 331, 659
BstACI GRCGYC 2 cut(s) 261, 303
BstBI TTCGAA 1 cut(s) 587
BstC8I GCNNGC 2 cut(s) 274, 318
BstDEI CTNAG 4 cut(s) 10, 417, 773, 1113
BstDSI CCRYGG 2 cut(s) 264, 900
BstF5I GGATG 2 cut(s) 820, 1096
BstMAI GTCTC 2 cut(s) 407, 870
BstMWI GCNNNNNNNGC 5 cut(s) 118, 127, 269, 317, 542
BstNI CCWGG 2 cut(s) 789, 1132
BstPAI GACNNNNGTC 1 cut(s) 187
BstSCI CCNGG 4 cut(s) 320, 787, 1012, 1130
BstSFI CTRYAG 2 cut(s) 216, 1151
BstSLI GKGCMC 1 cut(s) 148
BstV1I GCAGC 2 cut(s) 18, 816
BstV2I GAAGAC 2 cut(s) 187, 650
BsuRI GGCC 6 cut(s) 272, 276, 320, 792, 863, 1127
BtgI CCRYGG 2 cut(s) 264, 900
BtrI CACGTC 1 cut(s) 656
BtsCI GGATG 2 cut(s) 820, 1096
BtsI GCAGTG 2 cut(s) 462, 629
BtsIMutI CAGTG 2 cut(s) 462, 629
Cac8I GCNNGC 2 cut(s) 274, 318
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 2 cut(s) 47, 275
CseI GACGC 2 cut(s) 269, 292
Csp6I GTAC 3 cut(s) 843, 944, 1072
CviAII CATG 3 cut(s) 265, 607, 901
CviQI GTAC 3 cut(s) 843, 944, 1072
DdeI CTNAG 4 cut(s) 10, 417, 773, 1113
DraI TTTAAA 1 cut(s) 910
DraIII CACNNNGTG 1 cut(s) 799
DriI GACNNNNNGTC 1 cut(s) 607
EaeI YGGCCR 2 cut(s) 270, 861
Eam1104I CTCTTC 2 cut(s) 331, 659
Eam1105I GACNNNNNGTC 1 cut(s) 607
EarI CTCTTC 2 cut(s) 331, 659
Eco130I CCWWGG 3 cut(s) 264, 900, 1169
Eco24I GRGCYC 1 cut(s) 900
Eco32I GATATC 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 47
Eco57I CTGAAG 2 cut(s) 644, 716
Eco88I CYCGRG 1 cut(s) 880
EcoRII CCWGG 2 cut(s) 787, 1130
EcoRV GATATC 1 cut(s) 174
EcoT14I CCWWGG 3 cut(s) 264, 900, 1169
EcoT22I ATGCAT 1 cut(s) 570
EcoT38I GRGCYC 1 cut(s) 900
ErhI CCWWGG 3 cut(s) 264, 900, 1169
FaeI CATG 3 cut(s) 268, 610, 904
FaqI GGGAC 2 cut(s) 60, 905
FatI CATG 3 cut(s) 264, 606, 900
FauI CCCGC 2 cut(s) 323, 988
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 2 cut(s) 7, 830
FokI GGATG 2 cut(s) 827, 1103
FriOI GRGCYC 1 cut(s) 900
FseI GGCCGGCC 1 cut(s) 276
Fsp4HI GCNGC 2 cut(s) 7, 830
GluI GCNGC 2 cut(s) 7, 830
GsaI CCCAGC 1 cut(s) 540
HaeIII GGCC 6 cut(s) 272, 276, 320, 792, 863, 1127
HapII CCGG 4 cut(s) 273, 321, 594, 1013
HgaI GACGC 2 cut(s) 269, 292
Hin1I GRCGYC 2 cut(s) 261, 303
Hin1II CATG 3 cut(s) 268, 610, 904
HincII GTYRAC 2 cut(s) 207, 229
HindII GTYRAC 2 cut(s) 207, 229
HinfI GANTC 5 cut(s) 88, 280, 727, 878, 1144
HpaII CCGG 4 cut(s) 273, 321, 594, 1013
HphI GGTGA 5 cut(s) 169, 248, 266, 772, 773
Hpy166II GTNNAC 5 cut(s) 207, 229, 445, 627, 802
Hpy188I TCNGA 6 cut(s) 23, 193, 412, 580, 663, 735
Hpy188III TCNNGA 5 cut(s) 31, 177, 594, 812, 875
Hpy8I GTNNAC 5 cut(s) 207, 229, 445, 627, 802
Hpy99I CGWCG 3 cut(s) 214, 263, 308
HpyAV CCTTC 3 cut(s) 148, 320, 556
HpyCH4III ACNGT 3 cut(s) 842, 847, 987
HpyCH4IV ACGT 4 cut(s) 655, 690, 820, 942
HpyCH4V TGCA 7 cut(s) 6, 199, 218, 316, 568, 622, 742
HpyF10VI GCNNNNNNNGC 5 cut(s) 118, 127, 269, 317, 542
HpyF3I CTNAG 4 cut(s) 10, 417, 773, 1113
HpySE526I ACGT 4 cut(s) 655, 690, 820, 942
Hsp92I GRCGYC 2 cut(s) 261, 303
Hsp92II CATG 3 cut(s) 268, 610, 904
Kpn2I TCCGGA 1 cut(s) 593
KroI GCCGGC 1 cut(s) 272
KroNI GCCGGC 1 cut(s) 274
LmnI GCTCC 2 cut(s) 126, 963
Lsp1109I GCAGC 2 cut(s) 18, 816
LweI GCATC 3 cut(s) 186, 577, 729
MaeII ACGT 4 cut(s) 655, 690, 820, 942
MaeIII GTNAC 3 cut(s) 166, 254, 938
MhlI GDGCHC 2 cut(s) 148, 900
MluCI AATT 7 cut(s) 102, 463, 471, 506, 850, 867, 1005
MlyI GAGTC 2 cut(s) 274, 872
MmeI TCCRAC 2 cut(s) 65, 171
Mph1103I ATGCAT 1 cut(s) 570
MroI TCCGGA 1 cut(s) 593
MroNI GCCGGC 1 cut(s) 272
MroXI GAANNNNTTC 1 cut(s) 92
MseI TTAA 4 cut(s) 470, 504, 684, 909
MspI CCGG 4 cut(s) 273, 321, 594, 1013
MspR9I CCNGG 4 cut(s) 322, 789, 1014, 1132
MssI GTTTAAAC 1 cut(s) 910
MvaI CCWGG 2 cut(s) 789, 1132
MwoI GCNNNNNNNGC 5 cut(s) 118, 127, 269, 317, 542
NaeI GCCGGC 1 cut(s) 274
NciI CCSGG 2 cut(s) 322, 1014
NcoI CCATGG 2 cut(s) 264, 900
NgoMIV GCCGGC 1 cut(s) 272
NlaIII CATG 3 cut(s) 268, 610, 904
NlaIV GGNNCC 1 cut(s) 48
NmuCI GTSAC 1 cut(s) 254
NsiI ATGCAT 1 cut(s) 570
NspV TTCGAA 1 cut(s) 587
PaeR7I CTCGAG 1 cut(s) 880
PcsI WCGNNNNNNNCGW 1 cut(s) 584
PdiI GCCGGC 1 cut(s) 274
PdmI GAANNNNTTC 1 cut(s) 92
PfeI GAWTC 3 cut(s) 88, 727, 1144
PflMI CCANNNNNTGG 1 cut(s) 799
PfoI TCCNGGA 1 cut(s) 1012
PkrI GCNGC 2 cut(s) 8, 831
PleI GAGTC 2 cut(s) 274, 872
PmeI GTTTAAAC 1 cut(s) 910
PpsI GAGTC 2 cut(s) 274, 872
PshAI GACNNNNGTC 1 cut(s) 187
Psp1406I AACGTT 1 cut(s) 690
Psp6I CCWGG 2 cut(s) 787, 1130
PspFI CCCAGC 1 cut(s) 536
PspGI CCWGG 2 cut(s) 787, 1130
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 2 cut(s) 47, 275
PspXI VCTCGAGB 1 cut(s) 880
PstI CTGCAG 1 cut(s) 220
RigI GGCCGGCC 1 cut(s) 276
RsaI GTAC 3 cut(s) 844, 945, 1073
RsaNI GTAC 3 cut(s) 843, 944, 1072
SaqAI TTAA 4 cut(s) 470, 504, 684, 909
SatI GCNGC 2 cut(s) 7, 830
Sau96I GGNCC 2 cut(s) 47, 275
SchI GAGTC 2 cut(s) 274, 872
ScrFI CCNGG 4 cut(s) 322, 789, 1014, 1132
SduI GDGCHC 2 cut(s) 148, 900
SfaNI GCATC 3 cut(s) 186, 577, 729
SfcI CTRYAG 2 cut(s) 216, 1151
Sfr274I CTCGAG 1 cut(s) 880
SfuI TTCGAA 1 cut(s) 587
SinI GGWCC 1 cut(s) 47
SlaI CTCGAG 1 cut(s) 880
SmlI CTYRAG 1 cut(s) 880
SmoI CTYRAG 1 cut(s) 880
Sse9I AATT 7 cut(s) 102, 463, 471, 506, 850, 867, 1005
SsiI CCGC 4 cut(s) 234, 330, 755, 995
StyD4I CCNGG 4 cut(s) 320, 787, 1012, 1130
StyI CCWWGG 3 cut(s) 264, 900, 1169
TaaI ACNGT 3 cut(s) 842, 847, 987
TaiI ACGT 4 cut(s) 658, 693, 823, 945
TaqI TCGA 5 cut(s) 106, 587, 611, 874, 881
TaqII GACCGA 2 cut(s) 64, 199
TasI AATT 7 cut(s) 102, 463, 471, 506, 850, 867, 1005
TatI WGTACW 1 cut(s) 842
TfiI GAWTC 3 cut(s) 88, 727, 1144
Tru1I TTAA 4 cut(s) 470, 504, 684, 909
Tru9I TTAA 4 cut(s) 470, 504, 684, 909
TscAI CASTG 2 cut(s) 462, 629
TseFI GTSAC 1 cut(s) 254
TseI GCWGC 2 cut(s) 6, 829
Tsp45I GTSAC 1 cut(s) 254
TspDTI ATGAA 4 cut(s) 127, 567, 941, 1154
TspGWI ACGGA 1 cut(s) 65
TspRI CASTG 2 cut(s) 462, 629
Van91I CCANNNNNTGG 1 cut(s) 799
VpaK11BI GGWCC 1 cut(s) 47
XapI RAATTY 3 cut(s) 102, 850, 1005
XhoI CTCGAG 1 cut(s) 880
XmiI GTMKAC 1 cut(s) 444
XmnI GAANNNNTTC 1 cut(s) 92
Zsp2I ATGCAT 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.