Rw2G029880

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008149
Physical Location & Seq
Forward (+)
48526363 .. 48531776
5414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G029880.1

Sequence Viewer

Length: 1374 bp
ATGGAAATGATGTGGTTCCTCCTCTTCATCTTCTCCCTCTTATTCACTTTGCTGTTCAAATCCCTTCTCTTTTCCTCTAAACCCTCCACCAAACTCGACGGCACTATTCTACCGCCGGGACCCACCTCGATTCCCATAATCGGAAACTTCATATGGCTCTACAAACTAGCTTCCGGAATCGAACCCATACTTGTTGACCTCCACGGCAACGCCGTGTCTGCGGACCGCCCTCCTCCCCAGTCCATCACTAAGCTCATCACTAACAACAACCGTGTCATCTTCAGCAGCGGCTACGGCCCAACTTGGCGTCTCCTCCGCTGTAACCTCATATCCGAGGTTCTAAACCCTTCTCGGCTCAAGTCCTATGCTCCTTCTCGAAAATGGGCTTTAGATATCCTCACCAACCAGCTCGATCAGTCCGTATCATCAAACGGACCTGTCGTCGAAGTTTTAGAACACTTCCGGATCTCCATTACTTCTTTGTTCGTGTATATGACTTTTGGCGTGAAGGTGGAGGATGAGGCGAAGATGAAAGAAATCATCGATACCCTAAACTTGGGACCCAGGTGGTTGAACAAGATTTTGTTCCGTAAGGGTTGGAATCAGTTCTACGAAATTCGAAAGCAACAAAAGGACGTCCTGCTCCCTTTCATACAGGCACGAAAGAAGGTTAAACAAGAAAGAGTAAATGAGAACAACGATGACTTCATGATAGCTTATGTTGATACATTAATGGATTTAGAAGACCCTCTCGAGAACAGAGAATTCAATGAAGGAGAACTAGTGGATCAATGCTCCGAGGTCTTGAACTCCGGCACGGACACCATGTCAGTAACATTGCAGTGGACGATGGCGAATGTTGTGGGGGTTGGAAAGGAAGAAATGAAACAGGAGGATCTTCAGAAGATGCACTATTTGAAAGCAGTGGTGATGGAGGCTCTAAGGCTTCACCCACCAGGCCACTTTGTGGTTCCACACTCGCTTACAGAGGACATTGTTCTGTATGGACGTTATATGCTGCCAAAGAACTGTACTGTGAATTTCATGGTTGCCGAAATGGGTCGGGACCCGAGTGTTTGGGACGAGCCCATAGAGTTTAAGCCTGAGAGGTTTCTGAGTGATGGAGATAATGTACAGTGTGATATAACAGGGAGTAAAGAGATTAAGATGATACCTTTTGGGGCTGGGAGGAGAATATGTCCGGGGTGGGGTTTGTCCATGCTTCTTCTGGAGTATTTGGTGGCAAATTTGATTTGGAAGTACGAGTGGAAAGCTGTGGATGAAGTTGGTGTTGATTTGACTGAGAAGCAAGAGGCCACCTGGGTGATGAAGAATCCATTGCGGGCCCATATATCGCCAAGGTTGAACAAGTGA

Protein Analysis

457

Amino Acids

52.41

Weight (kDa)

5.92

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 71 - 445 7.5e-57 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 639
AccB7I CCANNNNNTGG 1 cut(s) 967
AccIII TCCGGA 2 cut(s) 173, 462
AciI CCGC 6 cut(s) 113, 221, 226, 288, 316, 1342
AclWI GGATC 3 cut(s) 473, 795, 903
AcsI RAATTY 4 cut(s) 615, 764, 1039, 1246
AcuI CTGAAG 2 cut(s) 265, 884
AcyI GRCGYC 2 cut(s) 307, 636
AdeI CACNNNGTG 1 cut(s) 967
AfaI GTAC 3 cut(s) 1033, 1134, 1262
AfiI CCNNNNNNNGG 4 cut(s) 140, 556, 967, 1208
AgsI TTSAA 6 cut(s) 58, 574, 769, 808, 919, 1366
AhdI GACNNNNNGTC 2 cut(s) 440, 826
AhlI ACTAGT 1 cut(s) 781
AjnI CCWGG 3 cut(s) 563, 955, 1319
AleI CACNNNNGTG 1 cut(s) 1322
AluBI AGCT 5 cut(s) 170, 253, 409, 716, 1274
AluI AGCT 5 cut(s) 170, 253, 409, 716, 1274
Alw26I GTCTC 1 cut(s) 314
AlwI GGATC 3 cut(s) 473, 795, 903
Ama87I CYCGRG 2 cut(s) 752, 1069
Aor13HI TCCGGA 2 cut(s) 173, 462
AoxI GGCC 4 cut(s) 295, 958, 1314, 1344
ApaI GGGCCC 1 cut(s) 1348
ApeKI GCWGC 2 cut(s) 285, 1018
ApoI RAATTY 4 cut(s) 615, 764, 1039, 1246
AseI ATTAAT 1 cut(s) 731
Asp700I GAANNNNTTC 1 cut(s) 605
AspS9I GGNCC 8 cut(s) 119, 223, 296, 434, 560, 1066, 1344, 1345
AsuC2I CCSGG 2 cut(s) 117, 1203
AsuHPI GGTGA 4 cut(s) 391, 940, 941, 1336
AsuII TTCGAA 1 cut(s) 619
AvaI CYCGRG 2 cut(s) 752, 1069
AvaII GGWCC 5 cut(s) 119, 223, 434, 560, 1066
BaeGI GKGCMC 1 cut(s) 1348
BanII GRGCYC 2 cut(s) 1089, 1348
BbsI GAAGAC 1 cut(s) 750
BbvI GCAGC 2 cut(s) 297, 1005
BccI CCATC 4 cut(s) 251, 844, 925, 1115
BceAI ACGGC 4 cut(s) 115, 197, 220, 310
BciT130I CCWGG 3 cut(s) 565, 957, 1321
BcnI CCSGG 2 cut(s) 117, 1203
BcoDI GTCTC 1 cut(s) 314
BcuI ACTAGT 1 cut(s) 781
BfaI CTAG 2 cut(s) 167, 782
BisI GCNGC 3 cut(s) 286, 289, 1019
BlsI GCNGC 3 cut(s) 287, 290, 1020
Bme1390I CCNGG 5 cut(s) 117, 565, 957, 1203, 1321
Bme18I GGWCC 5 cut(s) 119, 223, 434, 560, 1066
BmeRI GACNNNNNGTC 2 cut(s) 440, 826
BmeT110I CYCGRG 2 cut(s) 752, 1069
BmgT120I GGNCC 8 cut(s) 119, 223, 296, 434, 560, 1066, 1344, 1345
BmiI GGNNCC 9 cut(s) 17, 120, 121, 561, 562, 972, 1067, 1068, 1346
BmrFI CCNGG 5 cut(s) 117, 565, 957, 1203, 1321
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 1 cut(s) 897
BmuI ACTGGG 1 cut(s) 232
BpiI GAAGAC 1 cut(s) 750
BpmI CTGGAG 1 cut(s) 1250
Bpu14I TTCGAA 1 cut(s) 619
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 2 cut(s) 117, 1203
Bsa29I ATCGAT 1 cut(s) 543
BsaHI GRCGYC 2 cut(s) 307, 636
BsaJI CCNNGG 7 cut(s) 202, 333, 563, 798, 1202, 1320, 1358
BsaWI WCCGGW 2 cut(s) 173, 462
BsaXI ACNNNNNCTCC 6 cut(s) 768, 798, 1116, 1146, 1183, 1213
Bsc4I CCNNNNNNNGG 4 cut(s) 140, 556, 967, 1208
Bse1I ACTGG 1 cut(s) 238
Bse3DI GCAATG 2 cut(s) 836, 1337
BseAI TCCGGA 2 cut(s) 173, 462
BseBI CCWGG 3 cut(s) 565, 957, 1321
BseCI ATCGAT 1 cut(s) 543
BseDI CCNNGG 7 cut(s) 202, 333, 563, 798, 1202, 1320, 1358
BseGI GGATG 2 cut(s) 523, 1285
BseLI CCNNNNNNNGG 4 cut(s) 140, 556, 967, 1208
BseMI GCAATG 2 cut(s) 836, 1337
BseMII CTCAG 3 cut(s) 1095, 1106, 1293
BseNI ACTGG 1 cut(s) 238
BseRI GAGGAG 4 cut(s) 11, 222, 302, 1204
BseSI GKGCMC 1 cut(s) 1348
BseXI GCAGC 2 cut(s) 297, 1005
BseYI CCCAGC 1 cut(s) 1184
BshFI GGCC 4 cut(s) 297, 960, 1316, 1346
BshVI ATCGAT 1 cut(s) 543
BsiHKCI CYCGRG 2 cut(s) 752, 1069
BsiSI CCGG 5 cut(s) 116, 174, 463, 813, 1202
BslFI GGGAC 4 cut(s) 132, 573, 1079, 1094
BslI CCNNNNNNNGG 4 cut(s) 140, 556, 967, 1208
BsmAI GTCTC 1 cut(s) 314
BsmBI CGTCTC 1 cut(s) 314
BsmFI GGGAC 4 cut(s) 132, 573, 1079, 1094
BsnI GGCC 4 cut(s) 297, 960, 1316, 1346
BsoBI CYCGRG 2 cut(s) 752, 1069
Bsp119I TTCGAA 1 cut(s) 619
Bsp120I GGGCCC 1 cut(s) 1344
Bsp1286I GDGCHC 2 cut(s) 1089, 1348
Bsp13I TCCGGA 2 cut(s) 173, 462
Bsp1407I TGTACA 1 cut(s) 1132
Bsp143I GATC 4 cut(s) 412, 465, 787, 895
BspACI CCGC 6 cut(s) 113, 221, 226, 288, 316, 1342
BspANI GGCC 4 cut(s) 297, 960, 1316, 1346
BspCNI CTCAG 3 cut(s) 1096, 1107, 1294
BspDI ATCGAT 1 cut(s) 543
BspEI TCCGGA 2 cut(s) 173, 462
BspHI TCATGA 1 cut(s) 708
BspLI GGNNCC 9 cut(s) 17, 120, 121, 561, 562, 972, 1067, 1068, 1346
BspPI GGATC 3 cut(s) 473, 795, 903
BspT104I TTCGAA 1 cut(s) 619
BsrDI GCAATG 2 cut(s) 836, 1337
BsrGI TGTACA 1 cut(s) 1132
BsrI ACTGG 1 cut(s) 238
BssECI CCNNGG 7 cut(s) 202, 333, 563, 798, 1202, 1320, 1358
BssMI GATC 4 cut(s) 412, 465, 787, 895
BssNI GRCGYC 2 cut(s) 307, 636
BssT1I CCWWGG 1 cut(s) 1358
Bst2UI CCWGG 3 cut(s) 565, 957, 1321
Bst4CI ACNGT 4 cut(s) 272, 1031, 1036, 1137
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 2 cut(s) 307, 636
BstAUI TGTACA 1 cut(s) 1132
BstBI TTCGAA 1 cut(s) 619
BstC8I GCNNGC 1 cut(s) 1344
BstDEI CTNAG 5 cut(s) 249, 941, 1104, 1115, 1302
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 2 cut(s) 523, 1285
BstKTI GATC 4 cut(s) 415, 468, 790, 898
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 4 cut(s) 412, 465, 787, 895
BstMWI GCNNNNNNNGC 2 cut(s) 218, 294
BstNI CCWGG 3 cut(s) 565, 957, 1321
BstSCI CCNGG 5 cut(s) 115, 563, 955, 1201, 1319
BstSLI GKGCMC 1 cut(s) 1348
BstV1I GCAGC 2 cut(s) 297, 1005
BstV2I GAAGAC 1 cut(s) 750
BstX2I RGATCY 2 cut(s) 465, 895
BstYI RGATCY 2 cut(s) 465, 895
Bsu15I ATCGAT 1 cut(s) 543
BsuRI GGCC 4 cut(s) 297, 960, 1316, 1346
BsuTUI ATCGAT 1 cut(s) 543
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 2 cut(s) 523, 1285
BtsI GCAGTG 2 cut(s) 848, 930
BtsIMutI CAGTG 3 cut(s) 848, 930, 1142
Cac8I GCNNGC 1 cut(s) 1344
CciI TCATGA 1 cut(s) 708
Cfr13I GGNCC 8 cut(s) 119, 223, 296, 434, 560, 1066, 1344, 1345
ClaI ATCGAT 1 cut(s) 543
CpoI CGGWCCG 1 cut(s) 223
CseI GACGC 1 cut(s) 296
Csp6I GTAC 3 cut(s) 1032, 1133, 1261
CspI CGGWCCG 1 cut(s) 223
CviAII CATG 4 cut(s) 709, 826, 1045, 1219
CviQI GTAC 3 cut(s) 1032, 1133, 1261
DdeI CTNAG 5 cut(s) 249, 941, 1104, 1115, 1302
DpnI GATC 4 cut(s) 414, 467, 789, 897
DpnII GATC 4 cut(s) 412, 465, 787, 895
DraIII CACNNNGTG 1 cut(s) 967
DriI GACNNNNNGTC 2 cut(s) 440, 826
Eam1104I CTCTTC 1 cut(s) 29
Eam1105I GACNNNNNGTC 2 cut(s) 440, 826
EarI CTCTTC 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 1358
Eco24I GRGCYC 2 cut(s) 1089, 1348
Eco32I GATATC 1 cut(s) 394
Eco47I GGWCC 5 cut(s) 119, 223, 434, 560, 1066
Eco57I CTGAAG 2 cut(s) 265, 884
Eco88I CYCGRG 2 cut(s) 752, 1069
EcoO109I RGGNCCY 3 cut(s) 119, 560, 1066
EcoRI GAATTC 1 cut(s) 764
EcoRII CCWGG 3 cut(s) 563, 955, 1319
EcoRV GATATC 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 1358
EcoT38I GRGCYC 2 cut(s) 1089, 1348
ErhI CCWWGG 1 cut(s) 1358
Esp3I CGTCTC 1 cut(s) 314
FaeI CATG 4 cut(s) 712, 829, 1048, 1222
FaqI GGGAC 4 cut(s) 132, 573, 1079, 1094
FatI CATG 4 cut(s) 708, 825, 1044, 1218
FauI CCCGC 1 cut(s) 1335
FauNDI CATATG 1 cut(s) 152
Fnu4HI GCNGC 3 cut(s) 286, 289, 1019
FokI GGATG 2 cut(s) 530, 1292
FriOI GRGCYC 2 cut(s) 1089, 1348
Fsp4HI GCNGC 3 cut(s) 286, 289, 1019
FspBI CTAG 2 cut(s) 167, 782
GluI GCNGC 3 cut(s) 286, 289, 1019
GsaI CCCAGC 1 cut(s) 1188
GsuI CTGGAG 1 cut(s) 1250
HaeIII GGCC 4 cut(s) 297, 960, 1316, 1346
HapII CCGG 5 cut(s) 116, 174, 463, 813, 1202
HgaI GACGC 1 cut(s) 296
Hin1I GRCGYC 2 cut(s) 307, 636
Hin1II CATG 4 cut(s) 712, 829, 1048, 1222
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 4 cut(s) 130, 177, 601, 1333
HpaII CCGG 5 cut(s) 116, 174, 463, 813, 1202
HphI GGTGA 4 cut(s) 391, 940, 941, 1336
Hpy166II GTNNAC 2 cut(s) 196, 846
Hpy188I TCNGA 5 cut(s) 143, 334, 799, 903, 1116
Hpy188III TCNNGA 9 cut(s) 174, 375, 463, 709, 752, 754, 805, 1064, 1229
Hpy8I GTNNAC 2 cut(s) 196, 846
Hpy99I CGWCG 2 cut(s) 101, 446
HpyAV CCTTC 6 cut(s) 74, 357, 381, 502, 661, 767
HpyCH4III ACNGT 4 cut(s) 272, 1031, 1036, 1137
HpyCH4IV ACGT 2 cut(s) 636, 1009
HpyCH4V TGCA 2 cut(s) 841, 910
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 294
HpyF3I CTNAG 5 cut(s) 249, 941, 1104, 1115, 1302
HpySE526I ACGT 2 cut(s) 636, 1009
Hsp92I GRCGYC 2 cut(s) 307, 636
Hsp92II CATG 4 cut(s) 712, 829, 1048, 1222
KflI GGGWCCC 3 cut(s) 119, 560, 1066
Kpn2I TCCGGA 2 cut(s) 173, 462
Kzo9I GATC 4 cut(s) 412, 465, 787, 895
LmnI GCTCC 3 cut(s) 373, 648, 800
Lsp1109I GCAGC 2 cut(s) 297, 1005
LweI GCATC 1 cut(s) 897
MaeI CTAG 2 cut(s) 167, 782
MaeII ACGT 2 cut(s) 636, 1009
MaeIII GTNAC 2 cut(s) 320, 832
MalI GATC 4 cut(s) 414, 467, 789, 897
MboI GATC 4 cut(s) 412, 465, 787, 895
MflI RGATCY 2 cut(s) 465, 895
MhlI GDGCHC 2 cut(s) 1089, 1348
MluCI AATT 4 cut(s) 615, 764, 1039, 1246
MmeI TCCRAC 2 cut(s) 578, 850
MroI TCCGGA 2 cut(s) 173, 462
MroXI GAANNNNTTC 1 cut(s) 605
MseI TTAA 4 cut(s) 672, 731, 1098, 1164
MslI CAYNNNNRTG 2 cut(s) 841, 1322
MspA1I CMGCKG 2 cut(s) 288, 318
MspI CCGG 5 cut(s) 116, 174, 463, 813, 1202
MspR9I CCNGG 5 cut(s) 117, 565, 957, 1203, 1321
MvaI CCWGG 3 cut(s) 565, 957, 1321
MwoI GCNNNNNNNGC 2 cut(s) 218, 294
NciI CCSGG 2 cut(s) 117, 1203
NdeI CATATG 1 cut(s) 152
NdeII GATC 4 cut(s) 412, 465, 787, 895
NlaIII CATG 4 cut(s) 712, 829, 1048, 1222
NlaIV GGNNCC 9 cut(s) 17, 120, 121, 561, 562, 972, 1067, 1068, 1346
NmeAIII GCCGAG 1 cut(s) 331
NspV TTCGAA 1 cut(s) 619
OliI CACNNNNGTG 1 cut(s) 1322
PaeR7I CTCGAG 1 cut(s) 752
PagI TCATGA 1 cut(s) 708
PcsI WCGNNNNNNNCGW 3 cut(s) 210, 417, 438
PdmI GAANNNNTTC 1 cut(s) 605
PfeI GAWTC 4 cut(s) 130, 177, 601, 1333
PflMI CCANNNNNTGG 1 cut(s) 967
PkrI GCNGC 3 cut(s) 287, 290, 1020
PpuMI RGGWCCY 3 cut(s) 119, 560, 1066
PshBI ATTAAT 1 cut(s) 731
Psp5II RGGWCCY 3 cut(s) 119, 560, 1066
Psp6I CCWGG 3 cut(s) 563, 955, 1319
PspFI CCCAGC 1 cut(s) 1184
PspGI CCWGG 3 cut(s) 563, 955, 1319
PspN4I GGNNCC 9 cut(s) 17, 120, 121, 561, 562, 972, 1067, 1068, 1346
PspOMI GGGCCC 1 cut(s) 1344
PspPI GGNCC 8 cut(s) 119, 223, 296, 434, 560, 1066, 1344, 1345
PspPPI RGGWCCY 3 cut(s) 119, 560, 1066
PsuI RGATCY 2 cut(s) 465, 895
RsaI GTAC 3 cut(s) 1033, 1134, 1262
RsaNI GTAC 3 cut(s) 1032, 1133, 1261
RseI CAYNNNNRTG 2 cut(s) 841, 1322
Rsr2I CGGWCCG 1 cut(s) 223
RsrII CGGWCCG 1 cut(s) 223
SaqAI TTAA 4 cut(s) 672, 731, 1098, 1164
SatI GCNGC 3 cut(s) 286, 289, 1019
Sau3AI GATC 4 cut(s) 412, 465, 787, 895
Sau96I GGNCC 8 cut(s) 119, 223, 296, 434, 560, 1066, 1344, 1345
ScrFI CCNGG 5 cut(s) 117, 565, 957, 1203, 1321
SduI GDGCHC 2 cut(s) 1089, 1348
SfaNI GCATC 1 cut(s) 897
Sfr274I CTCGAG 1 cut(s) 752
SfuI TTCGAA 1 cut(s) 619
SinI GGWCC 5 cut(s) 119, 223, 434, 560, 1066
SlaI CTCGAG 1 cut(s) 752
SmiMI CAYNNNNRTG 2 cut(s) 841, 1322
SmlI CTYRAG 2 cut(s) 356, 752
SmoI CTYRAG 2 cut(s) 356, 752
SpeI ACTAGT 1 cut(s) 781
Sse9I AATT 4 cut(s) 615, 764, 1039, 1246
SsiI CCGC 6 cut(s) 113, 221, 226, 288, 316, 1342
SspMI CTAG 2 cut(s) 167, 782
StyD4I CCNGG 5 cut(s) 115, 563, 955, 1201, 1319
StyI CCWWGG 1 cut(s) 1358
TaaI ACNGT 4 cut(s) 272, 1031, 1036, 1137
TaiI ACGT 2 cut(s) 639, 1012
TaqI TCGA 9 cut(s) 96, 128, 180, 376, 411, 444, 543, 619, 753
TasI AATT 4 cut(s) 615, 764, 1039, 1246
TatI WGTACW 2 cut(s) 1031, 1132
TauI GCSGC 1 cut(s) 291
TfiI GAWTC 4 cut(s) 130, 177, 601, 1333
Tru1I TTAA 4 cut(s) 672, 731, 1098, 1164
Tru9I TTAA 4 cut(s) 672, 731, 1098, 1164
TscAI CASTG 3 cut(s) 848, 930, 1142
TseI GCWGC 2 cut(s) 285, 1018
TspGWI ACGGA 4 cut(s) 409, 447, 578, 833
TspRI CASTG 3 cut(s) 848, 930, 1142
Van91I CCANNNNNTGG 1 cut(s) 967
VpaK11BI GGWCC 5 cut(s) 119, 223, 434, 560, 1066
VspI ATTAAT 1 cut(s) 731
XapI RAATTY 4 cut(s) 615, 764, 1039, 1246
XcmI CCANNNNNNNNNTGG 1 cut(s) 1225
XhoI CTCGAG 1 cut(s) 752
XmnI GAANNNNTTC 1 cut(s) 605
XspI CTAG 2 cut(s) 167, 782
ZraI GACGTC 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.