Rw2G029930

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008149
Physical Location & Seq
Forward (+)
48653942 .. 48663830
9889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G029930.1

Sequence Viewer

Length: 1518 bp
ATGGAAATGATGTGGTTTCTCATCTTCATCCTCTCCCTCTTCATCGCTTTCCTCCTCAAATCTCTTCTCTTTCTCTCGAAACCCTCGGCCAAACTCGACGGTACTATTCTACCGCCAGGACCCACCTCGATTCCCATAATCGGAAACTTCATATGGCTCTTCAAACTGACCTCCAGAATCGAACCCATCCTTCTTGACCTCCACGCCAACTCTCACCCCGCCATTTTCATCAACGACCACTCACTCGCCCACCAAGTCTTGGTCCAAAACGGAGCAGTCTGTTCGGACCGCCCTCCTCCCCAGCCCATCTCCAAGCTCATCACTAGCGACAACCGTGTCATCTTCAGCAGTGGCTACGGCCCAACATGGCGTCTCCTCCGCCGAAACCTCATGTCCGAGGTTCTGAACCCTTCTAGGCTCAAGTCCTACGCTTCCTCCCGCAAATGGGCCTTGGACATCCTCACCAACCGTCTCGATCAGTCCGTATGGTCCAACGGACCAGTCGTCGAAGTTTTAGACCATTTTCGATTCTCCATAACTTCTTTGTTCGTGGATATGGTGTTTGGTGAGAAGGTGGAGGATGAGGCGAAGATGAAAGAAATTGCTGATGTGCAGCGTCGCTTTATGTTCGGTTTGGTCAGAGGTTTCCAGACCCTAAACTTGGGACCGAGATGGTTGAACAAGATTTTGTTCCGTAAGGGTTGGAATCAGTTCAACGAAATTCGACAGCAACAAAAGCACCTCCTGCTTCCATTCATACGGGCACGAAAGAAGGTGGTGAAACAAGAAATGGTAGATAAGAACAGCGATGAGTTCACAATAGCATATGTTGATACATTAATGGATTTAGAAGACCCCCGCGAGAAGAGAGCCTTCAATGAAGAAGAACTAATGCATCAATGCTCGGAGATTTTGAATTCCGGCACGGACACCATGTCCATAACATTGCAGTGGACCATGGCGAATGTAGTGAAATACCCACGTGTTCAGAAGAGGCTTGTTGAAGATATTAAAAAGGTTGTGGGTTGGAAAGATGAGGATCTTCAGAAGATGCACTACCTAAAAGCGGTGGTGATGGAGGCTCTAAGGCTTCACCCACCAGGCCACTATGTGGTGCCACACACGGTTACAGAGGACATTGTTCTGGCTGGACGTTATACGCTGCCAAAGAACTGTACTGTGAATTTTATGGTGGCCGAAATTGGTCGAGACTCGAGTGTGTGGGACGAGCCCATGGAGTTTAAACCTGAAAGGTTTATGAAAGATGGAGGTAACGTACAATGTGATATAACAGGGAGCAAGGAAATTATGATGATACCGTTTGGGGCGGGTAGGAGGATTTGTCCGGGATTGGGTTTGTCAATGCTTCTTTTGGAGTATTTGGTGGCAAATCTGGTTTGGAAGTACGAGTGGGAGGCTGTGGATGGAGTTGGTGTTGATTTGACTGAGAGACAAGAGGCCACCTGGGTAATGAAGAATCCACTACAGGCTCAAATATCCCCAAGGTTGAACAAATGA

Protein Analysis

505

Amino Acids

58.13

Weight (kDa)

8.61

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 38 - 495 4.2e-79 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 335
AccB1I GGYRCC 1 cut(s) 1114
AccB7I CCANNNNNTGG 2 cut(s) 259, 1111
AccII CGCG 1 cut(s) 861
AciI CCGC 8 cut(s) 113, 219, 289, 379, 439, 859, 1067, 1328
AclWI GGATC 1 cut(s) 1047
AcoI YGGCCR 2 cut(s) 87, 1194
AcsI RAATTY 3 cut(s) 720, 916, 1183
AcuI CTGAAG 2 cut(s) 328, 1028
AcvI CACGTG 1 cut(s) 983
AcyI GRCGYC 1 cut(s) 370
AdeI CACNNNGTG 1 cut(s) 1111
AfaI GTAC 4 cut(s) 103, 1177, 1278, 1406
AfiI CCNNNNNNNGG 8 cut(s) 140, 259, 444, 445, 661, 1066, 1111, 1352
AflIII ACRYGT 1 cut(s) 982
AgsI TTSAA 7 cut(s) 163, 679, 715, 877, 916, 1004, 1510
AhdI GACNNNNNGTC 2 cut(s) 503, 934
AjnI CCWGG 3 cut(s) 115, 1099, 1463
AluBI AGCT 1 cut(s) 316
AluI AGCT 1 cut(s) 316
Alw26I GTCTC 4 cut(s) 377, 476, 1203, 1444
AlwI GGATC 1 cut(s) 1047
Ama87I CYCGRG 1 cut(s) 1213
AoxI GGCC 6 cut(s) 87, 358, 447, 1102, 1194, 1458
ApeKI GCWGC 2 cut(s) 613, 1162
ApoI RAATTY 3 cut(s) 720, 916, 1183
AseI ATTAAT 1 cut(s) 839
Asp700I GAANNNNTTC 1 cut(s) 710
AspS9I GGNCC 9 cut(s) 119, 262, 286, 359, 447, 489, 497, 665, 954
AsuC2I CCSGG 1 cut(s) 1347
AsuHPI GGTGA 6 cut(s) 206, 454, 578, 790, 1084, 1085
AvaI CYCGRG 1 cut(s) 1213
AvaII GGWCC 7 cut(s) 119, 262, 286, 489, 497, 665, 954
BaeGI GKGCMC 1 cut(s) 766
BanI GGYRCC 1 cut(s) 1114
BanII GRGCYC 1 cut(s) 1233
BarI GAAGNNNNNNTAC 2 cut(s) 1040, 1072
BbrPI CACGTG 1 cut(s) 983
BbsI GAAGAC 1 cut(s) 858
BbvI GCAGC 2 cut(s) 625, 1149
BccI CCATC 6 cut(s) 194, 314, 666, 1069, 1259, 1418
BceAI ACGGC 1 cut(s) 373
BcgI CGANNNNNNTGC 2 cut(s) 264, 298
BciT130I CCWGG 3 cut(s) 117, 1101, 1465
BcnI CCSGG 1 cut(s) 1347
BcoDI GTCTC 4 cut(s) 377, 476, 1203, 1444
BfaI CTAG 2 cut(s) 324, 414
BfmI CTRYAG 1 cut(s) 1484
BisI GCNGC 2 cut(s) 614, 1163
BlsI GCNGC 2 cut(s) 615, 1164
Bme1390I CCNGG 4 cut(s) 117, 1101, 1347, 1465
Bme18I GGWCC 7 cut(s) 119, 262, 286, 489, 497, 665, 954
BmeRI GACNNNNNGTC 2 cut(s) 503, 934
BmeT110I CYCGRG 1 cut(s) 1213
BmgT120I GGNCC 9 cut(s) 119, 262, 286, 359, 447, 489, 497, 665, 954
BmiI GGNNCC 3 cut(s) 121, 666, 1116
BmrFI CCNGG 4 cut(s) 117, 1101, 1347, 1465
BmsI GCATC 2 cut(s) 904, 1041
BpiI GAAGAC 1 cut(s) 858
BpmI CTGGAG 1 cut(s) 157
BpuEI CTTGAG 1 cut(s) 404
BpuMI CCSGG 1 cut(s) 1347
BsaAI YACGTR 1 cut(s) 983
BsaBI GATNNNNATC 1 cut(s) 1038
BsaHI GRCGYC 1 cut(s) 370
BsaJI CCNNGG 7 cut(s) 84, 396, 450, 957, 1233, 1464, 1502
BsaXI ACNNNNNCTCC 8 cut(s) 419, 449, 1260, 1290, 1327, 1357, 1419, 1449
Bsc4I CCNNNNNNNGG 8 cut(s) 140, 259, 444, 445, 661, 1066, 1111, 1352
Bse1I ACTGG 1 cut(s) 500
Bse3DI GCAATG 1 cut(s) 944
Bse8I GATNNNNATC 1 cut(s) 1038
BseBI CCWGG 3 cut(s) 117, 1101, 1465
BseDI CCNNGG 7 cut(s) 84, 396, 450, 957, 1233, 1464, 1502
BseGI GGATG 5 cut(s) 27, 186, 456, 586, 1429
BseJI GATNNNNATC 1 cut(s) 1038
BseLI CCNNNNNNNGG 8 cut(s) 140, 259, 444, 445, 661, 1066, 1111, 1352
BseMI GCAATG 1 cut(s) 944
BseMII CTCAG 1 cut(s) 1437
BseNI ACTGG 1 cut(s) 500
BseRI GAGGAG 3 cut(s) 44, 285, 365
BseSI GKGCMC 1 cut(s) 766
BseXI GCAGC 2 cut(s) 625, 1149
BseYI CCCAGC 1 cut(s) 300
BsgI GTGCAG 1 cut(s) 632
Bsh1236I CGCG 1 cut(s) 861
BshFI GGCC 6 cut(s) 89, 360, 449, 1104, 1196, 1460
BshNI GGYRCC 1 cut(s) 1114
BsiHKCI CYCGRG 1 cut(s) 1213
BsiSI CCGG 2 cut(s) 921, 1346
BslFI GGGAC 2 cut(s) 678, 1238
BslI CCNNNNNNNGG 8 cut(s) 140, 259, 444, 445, 661, 1066, 1111, 1352
BsmAI GTCTC 4 cut(s) 377, 476, 1203, 1444
BsmBI CGTCTC 2 cut(s) 377, 476
BsmFI GGGAC 2 cut(s) 678, 1238
BsnI GGCC 6 cut(s) 89, 360, 449, 1104, 1196, 1460
BsoBI CYCGRG 1 cut(s) 1213
Bsp1286I GDGCHC 2 cut(s) 766, 1233
Bsp143I GATC 2 cut(s) 475, 1039
Bsp19I CCATGG 2 cut(s) 957, 1233
BspACI CCGC 8 cut(s) 113, 219, 289, 379, 439, 859, 1067, 1328
BspANI GGCC 6 cut(s) 89, 360, 449, 1104, 1196, 1460
BspCNI CTCAG 1 cut(s) 1438
BspFNI CGCG 1 cut(s) 861
BspLI GGNNCC 3 cut(s) 121, 666, 1116
BspPI GGATC 1 cut(s) 1047
BspQI GCTCTTC 1 cut(s) 164
BspT107I GGYRCC 1 cut(s) 1114
BsrDI GCAATG 1 cut(s) 944
BsrI ACTGG 1 cut(s) 500
BssECI CCNNGG 7 cut(s) 84, 396, 450, 957, 1233, 1464, 1502
BssMI GATC 2 cut(s) 475, 1039
BssNI GRCGYC 1 cut(s) 370
BssT1I CCWWGG 4 cut(s) 450, 957, 1233, 1502
Bst2UI CCWGG 3 cut(s) 117, 1101, 1465
Bst4CI ACNGT 7 cut(s) 101, 335, 470, 1126, 1175, 1180, 1320
Bst6I CTCTTC 5 cut(s) 44, 69, 164, 860, 986
BstACI GRCGYC 1 cut(s) 370
BstAPI GCANNNNNTGC 1 cut(s) 745
BstBAI YACGTR 1 cut(s) 983
BstDEI CTNAG 2 cut(s) 1085, 1446
BstDSI CCRYGG 2 cut(s) 957, 1233
BstF5I GGATG 5 cut(s) 27, 186, 456, 586, 1429
BstFNI CGCG 1 cut(s) 861
BstKTI GATC 2 cut(s) 478, 1042
BstMAI GTCTC 4 cut(s) 377, 476, 1203, 1444
BstMBI GATC 2 cut(s) 475, 1039
BstMWI GCNNNNNNNGC 2 cut(s) 736, 745
BstNI CCWGG 3 cut(s) 117, 1101, 1465
BstSCI CCNGG 4 cut(s) 115, 1099, 1345, 1463
BstSFI CTRYAG 1 cut(s) 1484
BstSLI GKGCMC 1 cut(s) 766
BstUI CGCG 1 cut(s) 861
BstV1I GCAGC 2 cut(s) 625, 1149
BstV2I GAAGAC 1 cut(s) 858
BstX2I RGATCY 1 cut(s) 1039
BstYI RGATCY 1 cut(s) 1039
BsuRI GGCC 6 cut(s) 89, 360, 449, 1104, 1196, 1460
BtgI CCRYGG 2 cut(s) 957, 1233
BtgZI GCGATG 2 cut(s) 28, 822
BtsCI GGATG 5 cut(s) 27, 186, 456, 586, 1429
BtsI GCAGTG 2 cut(s) 355, 956
BtsIMutI CAGTG 2 cut(s) 355, 956
Cfr13I GGNCC 9 cut(s) 119, 262, 286, 359, 447, 489, 497, 665, 954
CpoI CGGWCCG 1 cut(s) 286
CseI GACGC 2 cut(s) 359, 605
Csp6I GTAC 4 cut(s) 102, 1176, 1277, 1405
CspI CGGWCCG 1 cut(s) 286
CviAII CATG 5 cut(s) 366, 391, 934, 958, 1234
CviQI GTAC 4 cut(s) 102, 1176, 1277, 1405
DdeI CTNAG 2 cut(s) 1085, 1446
DpnI GATC 2 cut(s) 477, 1041
DpnII GATC 2 cut(s) 475, 1039
DraI TTTAAA 1 cut(s) 1243
DraIII CACNNNGTG 1 cut(s) 1111
DrdI GACNNNNNNGTC 1 cut(s) 335
DriI GACNNNNNGTC 2 cut(s) 503, 934
DseDI GACNNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 2 cut(s) 87, 1194
Eam1104I CTCTTC 5 cut(s) 44, 69, 164, 860, 986
Eam1105I GACNNNNNGTC 2 cut(s) 503, 934
EarI CTCTTC 5 cut(s) 44, 69, 164, 860, 986
EciI GGCGGA 1 cut(s) 368
Eco130I CCWWGG 4 cut(s) 450, 957, 1233, 1502
Eco24I GRGCYC 1 cut(s) 1233
Eco47I GGWCC 7 cut(s) 119, 262, 286, 489, 497, 665, 954
Eco57I CTGAAG 2 cut(s) 328, 1028
Eco72I CACGTG 1 cut(s) 983
Eco88I CYCGRG 1 cut(s) 1213
EcoO109I RGGNCCY 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 916
EcoRII CCWGG 3 cut(s) 115, 1099, 1463
EcoT14I CCWWGG 4 cut(s) 450, 957, 1233, 1502
EcoT22I ATGCAT 1 cut(s) 897
EcoT38I GRGCYC 1 cut(s) 1233
ErhI CCWWGG 4 cut(s) 450, 957, 1233, 1502
Esp3I CGTCTC 2 cut(s) 377, 476
FaeI CATG 5 cut(s) 369, 394, 937, 961, 1237
FalI AAGNNNNNCTT 2 cut(s) 857, 889
FaqI GGGAC 2 cut(s) 678, 1238
FatI CATG 5 cut(s) 365, 390, 933, 957, 1233
FauI CCCGC 4 cut(s) 226, 446, 866, 1321
FauNDI CATATG 2 cut(s) 152, 826
Fnu4HI GCNGC 2 cut(s) 614, 1163
FokI GGATG 5 cut(s) 14, 173, 443, 593, 1436
FriOI GRGCYC 1 cut(s) 1233
Fsp4HI GCNGC 2 cut(s) 614, 1163
FspBI CTAG 2 cut(s) 324, 414
GluI GCNGC 2 cut(s) 614, 1163
GsaI CCCAGC 1 cut(s) 304
GsuI CTGGAG 1 cut(s) 157
HaeIII GGCC 6 cut(s) 89, 360, 449, 1104, 1196, 1460
HapII CCGG 2 cut(s) 921, 1346
HgaI GACGC 2 cut(s) 359, 605
Hin1I GRCGYC 1 cut(s) 370
Hin1II CATG 5 cut(s) 369, 394, 937, 961, 1237
HinfI GANTC 6 cut(s) 130, 177, 528, 706, 1211, 1477
HpaII CCGG 2 cut(s) 921, 1346
HphI GGTGA 6 cut(s) 206, 454, 578, 790, 1084, 1085
Hpy166II GTNNAC 2 cut(s) 816, 954
Hpy188I TCNGA 8 cut(s) 143, 286, 397, 405, 641, 907, 990, 1047
Hpy188III TCNNGA 6 cut(s) 76, 174, 194, 473, 649, 1208
Hpy8I GTNNAC 2 cut(s) 816, 954
Hpy99I CGWCG 3 cut(s) 101, 509, 621
HpyAV CCTTC 5 cut(s) 200, 420, 565, 766, 883
HpyCH4III ACNGT 7 cut(s) 101, 335, 470, 1126, 1175, 1180, 1320
HpyCH4IV ACGT 3 cut(s) 982, 1153, 1275
HpyCH4V TGCA 4 cut(s) 613, 895, 949, 1054
HpyF10VI GCNNNNNNNGC 2 cut(s) 736, 745
HpyF3I CTNAG 2 cut(s) 1085, 1446
HpySE526I ACGT 3 cut(s) 982, 1153, 1275
Hsp92I GRCGYC 1 cut(s) 370
Hsp92II CATG 5 cut(s) 369, 394, 937, 961, 1237
Kzo9I GATC 2 cut(s) 475, 1039
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 2 cut(s) 272, 1296
Lsp1109I GCAGC 2 cut(s) 625, 1149
LweI GCATC 2 cut(s) 904, 1041
MaeI CTAG 2 cut(s) 324, 414
MaeII ACGT 3 cut(s) 982, 1153, 1275
MaeIII GTNAC 2 cut(s) 1126, 1271
MalI GATC 2 cut(s) 477, 1041
MboI GATC 2 cut(s) 475, 1039
MflI RGATCY 1 cut(s) 1039
MhlI GDGCHC 2 cut(s) 766, 1233
MluCI AATT 6 cut(s) 600, 720, 916, 1183, 1200, 1305
MlyI GAGTC 1 cut(s) 1205
MmeI TCCRAC 3 cut(s) 516, 683, 1007
Mph1103I ATGCAT 1 cut(s) 897
MroXI GAANNNNTTC 1 cut(s) 710
MseI TTAA 3 cut(s) 839, 1011, 1242
MslI CAYNNNNRTG 1 cut(s) 949
MspI CCGG 2 cut(s) 921, 1346
MspR9I CCNGG 4 cut(s) 117, 1101, 1347, 1465
MssI GTTTAAAC 1 cut(s) 1243
MvaI CCWGG 3 cut(s) 117, 1101, 1465
MvnI CGCG 1 cut(s) 861
MwoI GCNNNNNNNGC 2 cut(s) 736, 745
NciI CCSGG 1 cut(s) 1347
NcoI CCATGG 2 cut(s) 957, 1233
NdeI CATATG 2 cut(s) 152, 826
NdeII GATC 2 cut(s) 475, 1039
NlaIII CATG 5 cut(s) 369, 394, 937, 961, 1237
NlaIV GGNNCC 3 cut(s) 121, 666, 1116
NmeAIII GCCGAG 1 cut(s) 65
NsiI ATGCAT 1 cut(s) 897
PaeR7I CTCGAG 1 cut(s) 1213
PciSI GCTCTTC 1 cut(s) 164
PcsI WCGNNNNNNNCGW 2 cut(s) 480, 501
PdmI GAANNNNTTC 1 cut(s) 710
PfeI GAWTC 5 cut(s) 130, 177, 528, 706, 1477
PflMI CCANNNNNTGG 2 cut(s) 259, 1111
PfoI TCCNGGA 1 cut(s) 1345
PkrI GCNGC 2 cut(s) 615, 1164
PleI GAGTC 1 cut(s) 1205
PmaCI CACGTG 1 cut(s) 983
PmeI GTTTAAAC 1 cut(s) 1243
PmlI CACGTG 1 cut(s) 983
PpsI GAGTC 1 cut(s) 1205
Ppu21I YACGTR 1 cut(s) 983
PpuMI RGGWCCY 1 cut(s) 119
PshBI ATTAAT 1 cut(s) 839
Psp5II RGGWCCY 1 cut(s) 119
Psp6I CCWGG 3 cut(s) 115, 1099, 1463
PspCI CACGTG 1 cut(s) 983
PspFI CCCAGC 1 cut(s) 300
PspGI CCWGG 3 cut(s) 115, 1099, 1463
PspN4I GGNNCC 3 cut(s) 121, 666, 1116
PspPI GGNCC 9 cut(s) 119, 262, 286, 359, 447, 489, 497, 665, 954
PspPPI RGGWCCY 1 cut(s) 119
PspXI VCTCGAGB 1 cut(s) 1213
PsuI RGATCY 1 cut(s) 1039
RsaI GTAC 4 cut(s) 103, 1177, 1278, 1406
RsaNI GTAC 4 cut(s) 102, 1176, 1277, 1405
RseI CAYNNNNRTG 1 cut(s) 949
Rsr2I CGGWCCG 1 cut(s) 286
RsrII CGGWCCG 1 cut(s) 286
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 3 cut(s) 839, 1011, 1242
SatI GCNGC 2 cut(s) 614, 1163
Sau3AI GATC 2 cut(s) 475, 1039
Sau96I GGNCC 9 cut(s) 119, 262, 286, 359, 447, 489, 497, 665, 954
SchI GAGTC 1 cut(s) 1205
ScrFI CCNGG 4 cut(s) 117, 1101, 1347, 1465
SduI GDGCHC 2 cut(s) 766, 1233
SfaNI GCATC 2 cut(s) 904, 1041
SfcI CTRYAG 1 cut(s) 1484
Sfr274I CTCGAG 1 cut(s) 1213
SinI GGWCC 7 cut(s) 119, 262, 286, 489, 497, 665, 954
SlaI CTCGAG 1 cut(s) 1213
SmiMI CAYNNNNRTG 1 cut(s) 949
SmlI CTYRAG 2 cut(s) 419, 1213
SmoI CTYRAG 2 cut(s) 419, 1213
Sse9I AATT 6 cut(s) 600, 720, 916, 1183, 1200, 1305
SsiI CCGC 8 cut(s) 113, 219, 289, 379, 439, 859, 1067, 1328
SspMI CTAG 2 cut(s) 324, 414
StyD4I CCNGG 4 cut(s) 115, 1099, 1345, 1463
StyI CCWWGG 4 cut(s) 450, 957, 1233, 1502
TaaI ACNGT 7 cut(s) 101, 335, 470, 1126, 1175, 1180, 1320
TaiI ACGT 3 cut(s) 985, 1156, 1278
TaqII GACCGA 1 cut(s) 682
TasI AATT 6 cut(s) 600, 720, 916, 1183, 1200, 1305
TatI WGTACW 1 cut(s) 1175
TfiI GAWTC 5 cut(s) 130, 177, 528, 706, 1477
Tru1I TTAA 3 cut(s) 839, 1011, 1242
Tru9I TTAA 3 cut(s) 839, 1011, 1242
TscAI CASTG 2 cut(s) 355, 956
TseI GCWGC 2 cut(s) 613, 1162
TspDTI ATGAA 9 cut(s) 16, 31, 139, 217, 608, 745, 894, 1274, 1487
TspGWI ACGGA 5 cut(s) 285, 472, 510, 683, 941
TspRI CASTG 2 cut(s) 355, 956
Van91I CCANNNNNTGG 2 cut(s) 259, 1111
VpaK11BI GGWCC 7 cut(s) 119, 262, 286, 489, 497, 665, 954
VspI ATTAAT 1 cut(s) 839
XapI RAATTY 3 cut(s) 720, 916, 1183
XhoI CTCGAG 1 cut(s) 1213
XmnI GAANNNNTTC 1 cut(s) 710
XspI CTAG 2 cut(s) 324, 414
Zsp2I ATGCAT 1 cut(s) 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.