Rw0G010730

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00460
OG0008149
Physical Location & Seq
Forward (+)
9618 .. 20903
11286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G010730.1

Sequence Viewer

Length: 912 bp
ATGGTGTTTGGTGAGAAGGTGGAGGATGAGGCGAAGATGAAAGAAATTGCTGATGTGCAGCGTCGCTTTATGTTCGGTTTGGTCAGAGGTTTCCAGACCCTAAACTTGGGACCGAGATGGTTGAACAAGATTTTGTTCCGTAAGGGTTGGAATCAGTTCAACGAAATTCGACAGCAACAAAAGCACCTCCTGCTTCCTTTCATACGGGCACGAAAGAAGGTGGTGGTAAAAAATAGGGATGAAATTGCTGTTCACGATAGCATAATGGATTTAGAAGACCCCCGCGAGAAGAGAGCCTTCAATGAAGAAGAACTAATGCATCAATGCTCGGAGATTTTAAACTCCGGCACGGACACCATGTCCATAATATTACAGTGGACGATGGCGAATGTAGTGAAATACCCACGTGTTCAGAAGAGGCTTGTTGAAGATATTAAAAAGGTTGTGGGTCTCAGGACCGGCCCTGTTCAGAAGATGCACTACCTAAAAGCGGTGGTGATGGAGGCTCTAAGGCTTCACCCACCAGGCCACTATGTGGTGCCACACACGGTTACAGAGGACATTGTTCTGGCTGGACGTTATACGCTGCCAAAGAACTGTACTGTGAATTTTATGGTGGCCGAAATTGGTCGAGACTCGAGTGTGTGGGACGAGCCCATGGAGTTTAAACCTGAAAGGTTTATGAAAGATGGAGGTAACGTACAATGTGATATAACAGGGAGCAAGGAGATTATGATGATACCGTTTGGGGCGGGTAGGAGGATTTGTCCGGGATTGGGTTTGTCAATGCTTCTTTTGGAGTATTTGGAAGCTGCGGATGGAGTTGGTGTTGATTTGACTGAGAGACAAGAGGCCACCTGGGTAATGAAGAATCCACTACAGGCTCATATATCCCCAACGTTGAACAAATGA

Protein Analysis

303

Amino Acids

34.84

Weight (kDa)

8.78

Isoelectric Point (pI)

51.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 3 - 267 8e-55 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 538
AccB7I CCANNNNNTGG 1 cut(s) 535
AccII CGCG 1 cut(s) 285
AciI CCGC 4 cut(s) 283, 491, 752, 815
AclI AACGTT 1 cut(s) 899
AcoI YGGCCR 1 cut(s) 618
AcsI RAATTY 2 cut(s) 165, 607
AcvI CACGTG 1 cut(s) 407
AdeI CACNNNGTG 1 cut(s) 535
AfaI GTAC 2 cut(s) 601, 702
AfiI CCNNNNNNNGG 4 cut(s) 106, 490, 535, 776
AflIII ACRYGT 1 cut(s) 406
AgsI TTSAA 5 cut(s) 124, 160, 301, 428, 904
AhdI GACNNNNNGTC 1 cut(s) 358
AjnI CCWGG 2 cut(s) 523, 857
AluBI AGCT 1 cut(s) 812
AluI AGCT 1 cut(s) 812
Alw26I GTCTC 3 cut(s) 455, 627, 838
Ama87I CYCGRG 1 cut(s) 637
AoxI GGCC 4 cut(s) 460, 526, 618, 852
ApeKI GCWGC 3 cut(s) 58, 586, 812
ApoI RAATTY 2 cut(s) 165, 607
Asp700I GAANNNNTTC 1 cut(s) 155
AspS9I GGNCC 3 cut(s) 110, 456, 461
AsuC2I CCSGG 1 cut(s) 771
AsuHPI GGTGA 3 cut(s) 23, 508, 509
AvaI CYCGRG 1 cut(s) 637
AvaII GGWCC 2 cut(s) 110, 456
BaeGI GKGCMC 1 cut(s) 211
BanI GGYRCC 1 cut(s) 538
BanII GRGCYC 1 cut(s) 657
BarI GAAGNNNNNNTAC 2 cut(s) 464, 496
BbrPI CACGTG 1 cut(s) 407
BbsI GAAGAC 1 cut(s) 282
BbvI GCAGC 3 cut(s) 70, 573, 799
BccI CCATC 5 cut(s) 111, 376, 493, 683, 812
BciT130I CCWGG 2 cut(s) 525, 859
BcnI CCSGG 1 cut(s) 771
BcoDI GTCTC 3 cut(s) 455, 627, 838
BfmI CTRYAG 1 cut(s) 878
BisI GCNGC 3 cut(s) 59, 587, 813
BlsI GCNGC 3 cut(s) 60, 588, 814
Bme1390I CCNGG 3 cut(s) 525, 771, 859
Bme18I GGWCC 2 cut(s) 110, 456
BmeRI GACNNNNNGTC 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 637
BmgT120I GGNCC 3 cut(s) 110, 456, 461
BmiI GGNNCC 2 cut(s) 111, 540
BmrFI CCNGG 3 cut(s) 525, 771, 859
BmsI GCATC 2 cut(s) 328, 465
BpiI GAAGAC 1 cut(s) 282
BpuMI CCSGG 1 cut(s) 771
BsaAI YACGTR 1 cut(s) 407
BsaI GGTCTC 1 cut(s) 455
BsaJI CCNNGG 2 cut(s) 657, 858
BsaXI ACNNNNNCTCC 6 cut(s) 684, 714, 751, 781, 813, 843
Bsc4I CCNNNNNNNGG 4 cut(s) 106, 490, 535, 776
Bse118I RCCGGY 1 cut(s) 458
BseBI CCWGG 2 cut(s) 525, 859
BseDI CCNNGG 2 cut(s) 657, 858
BseGI GGATG 3 cut(s) 31, 244, 823
BseLI CCNNNNNNNGG 4 cut(s) 106, 490, 535, 776
BseMII CTCAG 2 cut(s) 466, 831
BseSI GKGCMC 1 cut(s) 211
BseXI GCAGC 3 cut(s) 70, 573, 799
BsgI GTGCAG 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 285
BshFI GGCC 4 cut(s) 462, 528, 620, 854
BshNI GGYRCC 1 cut(s) 538
BsiHKCI CYCGRG 1 cut(s) 637
BsiSI CCGG 3 cut(s) 345, 459, 770
BslFI GGGAC 2 cut(s) 123, 662
BslI CCNNNNNNNGG 4 cut(s) 106, 490, 535, 776
BsmAI GTCTC 3 cut(s) 455, 627, 838
BsmFI GGGAC 2 cut(s) 123, 662
BsnI GGCC 4 cut(s) 462, 528, 620, 854
Bso31I GGTCTC 1 cut(s) 455
BsoBI CYCGRG 1 cut(s) 637
Bsp1286I GDGCHC 2 cut(s) 211, 657
Bsp19I CCATGG 1 cut(s) 657
BspACI CCGC 4 cut(s) 283, 491, 752, 815
BspANI GGCC 4 cut(s) 462, 528, 620, 854
BspCNI CTCAG 2 cut(s) 465, 832
BspFNI CGCG 1 cut(s) 285
BspLI GGNNCC 2 cut(s) 111, 540
BspT107I GGYRCC 1 cut(s) 538
BspTNI GGTCTC 1 cut(s) 455
BsrFI RCCGGY 1 cut(s) 458
BssAI RCCGGY 1 cut(s) 458
BssECI CCNNGG 2 cut(s) 657, 858
BssT1I CCWWGG 1 cut(s) 657
Bst2UI CCWGG 2 cut(s) 525, 859
Bst4CI ACNGT 5 cut(s) 375, 550, 599, 604, 744
Bst6I CTCTTC 2 cut(s) 284, 410
BstAPI GCANNNNNTGC 1 cut(s) 190
BstBAI YACGTR 1 cut(s) 407
BstDEI CTNAG 3 cut(s) 452, 509, 840
BstDSI CCRYGG 1 cut(s) 657
BstF5I GGATG 3 cut(s) 31, 244, 823
BstFNI CGCG 1 cut(s) 285
BstMAI GTCTC 3 cut(s) 455, 627, 838
BstMWI GCNNNNNNNGC 2 cut(s) 181, 190
BstNI CCWGG 2 cut(s) 525, 859
BstSCI CCNGG 3 cut(s) 523, 769, 857
BstSFI CTRYAG 1 cut(s) 878
BstSLI GKGCMC 1 cut(s) 211
BstUI CGCG 1 cut(s) 285
BstV1I GCAGC 3 cut(s) 70, 573, 799
BstV2I GAAGAC 1 cut(s) 282
BsuRI GGCC 4 cut(s) 462, 528, 620, 854
BtgI CCRYGG 1 cut(s) 657
BtsCI GGATG 3 cut(s) 31, 244, 823
BtsIMutI CAGTG 1 cut(s) 380
Cfr10I RCCGGY 1 cut(s) 458
Cfr13I GGNCC 3 cut(s) 110, 456, 461
CseI GACGC 1 cut(s) 50
Csp6I GTAC 2 cut(s) 600, 701
CviAII CATG 2 cut(s) 358, 658
CviQI GTAC 2 cut(s) 600, 701
DdeI CTNAG 3 cut(s) 452, 509, 840
DraI TTTAAA 2 cut(s) 339, 667
DraIII CACNNNGTG 1 cut(s) 535
DriI GACNNNNNGTC 1 cut(s) 358
EaeI YGGCCR 1 cut(s) 618
Eam1104I CTCTTC 2 cut(s) 284, 410
Eam1105I GACNNNNNGTC 1 cut(s) 358
EarI CTCTTC 2 cut(s) 284, 410
Eco130I CCWWGG 1 cut(s) 657
Eco24I GRGCYC 1 cut(s) 657
Eco31I GGTCTC 1 cut(s) 455
Eco47I GGWCC 2 cut(s) 110, 456
Eco72I CACGTG 1 cut(s) 407
Eco88I CYCGRG 1 cut(s) 637
EcoRII CCWGG 2 cut(s) 523, 857
EcoT14I CCWWGG 1 cut(s) 657
EcoT22I ATGCAT 1 cut(s) 321
EcoT38I GRGCYC 1 cut(s) 657
ErhI CCWWGG 1 cut(s) 657
FaeI CATG 2 cut(s) 361, 661
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FaqI GGGAC 2 cut(s) 123, 662
FatI CATG 2 cut(s) 357, 657
FauI CCCGC 2 cut(s) 290, 745
Fnu4HI GCNGC 3 cut(s) 59, 587, 813
FokI GGATG 3 cut(s) 38, 251, 830
FriOI GRGCYC 1 cut(s) 657
Fsp4HI GCNGC 3 cut(s) 59, 587, 813
GluI GCNGC 3 cut(s) 59, 587, 813
HaeIII GGCC 4 cut(s) 462, 528, 620, 854
HapII CCGG 3 cut(s) 345, 459, 770
HgaI GACGC 1 cut(s) 50
Hin1II CATG 2 cut(s) 361, 661
HinfI GANTC 3 cut(s) 151, 635, 871
HpaII CCGG 3 cut(s) 345, 459, 770
HphI GGTGA 3 cut(s) 23, 508, 509
Hpy166II GTNNAC 2 cut(s) 253, 378
Hpy188I TCNGA 4 cut(s) 86, 331, 414, 471
Hpy188III TCNNGA 4 cut(s) 94, 254, 454, 632
Hpy8I GTNNAC 2 cut(s) 253, 378
Hpy99I CGWCG 1 cut(s) 66
HpyAV CCTTC 3 cut(s) 10, 211, 307
HpyCH4III ACNGT 5 cut(s) 375, 550, 599, 604, 744
HpyCH4IV ACGT 4 cut(s) 406, 577, 699, 899
HpyCH4V TGCA 3 cut(s) 58, 319, 478
HpyF10VI GCNNNNNNNGC 2 cut(s) 181, 190
HpyF3I CTNAG 3 cut(s) 452, 509, 840
HpySE526I ACGT 4 cut(s) 406, 577, 699, 899
Hsp92II CATG 2 cut(s) 361, 661
LmnI GCTCC 1 cut(s) 720
Lsp1109I GCAGC 3 cut(s) 70, 573, 799
LweI GCATC 2 cut(s) 328, 465
MaeII ACGT 4 cut(s) 406, 577, 699, 899
MaeIII GTNAC 2 cut(s) 550, 695
MboII GAAGA 9 cut(s) 46, 287, 301, 317, 320, 427, 440, 484, 880
MhlI GDGCHC 2 cut(s) 211, 657
MluCI AATT 5 cut(s) 45, 165, 243, 607, 624
MlyI GAGTC 1 cut(s) 629
MmeI TCCRAC 1 cut(s) 128
Mph1103I ATGCAT 1 cut(s) 321
MroXI GAANNNNTTC 1 cut(s) 155
MseI TTAA 3 cut(s) 338, 435, 666
MspI CCGG 3 cut(s) 345, 459, 770
MspR9I CCNGG 3 cut(s) 525, 771, 859
MssI GTTTAAAC 1 cut(s) 667
MvaI CCWGG 2 cut(s) 525, 859
MvnI CGCG 1 cut(s) 285
MwoI GCNNNNNNNGC 2 cut(s) 181, 190
NciI CCSGG 1 cut(s) 771
NcoI CCATGG 1 cut(s) 657
NlaIII CATG 2 cut(s) 361, 661
NlaIV GGNNCC 2 cut(s) 111, 540
NsiI ATGCAT 1 cut(s) 321
PaeR7I CTCGAG 1 cut(s) 637
PdmI GAANNNNTTC 1 cut(s) 155
PfeI GAWTC 2 cut(s) 151, 871
PflMI CCANNNNNTGG 1 cut(s) 535
PfoI TCCNGGA 1 cut(s) 769
PkrI GCNGC 3 cut(s) 60, 588, 814
PleI GAGTC 1 cut(s) 629
PmaCI CACGTG 1 cut(s) 407
PmeI GTTTAAAC 1 cut(s) 667
PmlI CACGTG 1 cut(s) 407
PpsI GAGTC 1 cut(s) 629
Ppu21I YACGTR 1 cut(s) 407
Psp1406I AACGTT 1 cut(s) 899
Psp6I CCWGG 2 cut(s) 523, 857
PspCI CACGTG 1 cut(s) 407
PspGI CCWGG 2 cut(s) 523, 857
PspN4I GGNNCC 2 cut(s) 111, 540
PspPI GGNCC 3 cut(s) 110, 456, 461
PspXI VCTCGAGB 1 cut(s) 637
RsaI GTAC 2 cut(s) 601, 702
RsaNI GTAC 2 cut(s) 600, 701
SaqAI TTAA 3 cut(s) 338, 435, 666
SatI GCNGC 3 cut(s) 59, 587, 813
Sau96I GGNCC 3 cut(s) 110, 456, 461
SchI GAGTC 1 cut(s) 629
ScrFI CCNGG 3 cut(s) 525, 771, 859
SduI GDGCHC 2 cut(s) 211, 657
SfaNI GCATC 2 cut(s) 328, 465
SfcI CTRYAG 1 cut(s) 878
Sfr274I CTCGAG 1 cut(s) 637
SinI GGWCC 2 cut(s) 110, 456
SlaI CTCGAG 1 cut(s) 637
SmlI CTYRAG 1 cut(s) 637
SmoI CTYRAG 1 cut(s) 637
Sse9I AATT 5 cut(s) 45, 165, 243, 607, 624
SsiI CCGC 4 cut(s) 283, 491, 752, 815
SspI AATATT 1 cut(s) 369
StyD4I CCNGG 3 cut(s) 523, 769, 857
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 5 cut(s) 375, 550, 599, 604, 744
TaiI ACGT 4 cut(s) 409, 580, 702, 902
TaqI TCGA 3 cut(s) 169, 631, 638
TaqII GACCGA 1 cut(s) 127
TasI AATT 5 cut(s) 45, 165, 243, 607, 624
TatI WGTACW 1 cut(s) 599
TfiI GAWTC 2 cut(s) 151, 871
Tru1I TTAA 3 cut(s) 338, 435, 666
Tru9I TTAA 3 cut(s) 338, 435, 666
TscAI CASTG 1 cut(s) 380
TseI GCWGC 3 cut(s) 58, 586, 812
TspDTI ATGAA 6 cut(s) 53, 190, 255, 318, 698, 881
TspGWI ACGGA 2 cut(s) 128, 365
TspRI CASTG 1 cut(s) 380
Van91I CCANNNNNTGG 1 cut(s) 535
VpaK11BI GGWCC 2 cut(s) 110, 456
XapI RAATTY 2 cut(s) 165, 607
XhoI CTCGAG 1 cut(s) 637
XmnI GAANNNNTTC 1 cut(s) 155
Zsp2I ATGCAT 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.