RchiOBHm_Chr2g0175231

At3g27210-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008381
Physical Location & Seq
Forward (+)
87530373 .. 87533652
3280 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54232

Sequence Viewer

Length: 708 bp
ATGGGTACGTGTGCTTCGGCTCATAGGGAATCCGGGTCGGCCATGAAACTCGGAATGTCTTTCGGGTCCAAACCGGACAAGCTCTCCTCTATTCTACCGTCCCCGGTCAAAGAGAAACCGTCGACAAACGGTGGCCGTCCGATCAACGATGAATGGTCGCCTGCTCGCCCCACAGCCACGGCTTTCCGAGACTACGGTAGTAAAGATGAGGCCTTCTTTGATTCCAAGCCTTGGTTGGACTCAGATTGTGAAGATGACTTCTACAGTGTCAATGGTGACTTTACTCCATCTCGTGGCAACACTCCGGTCCATCAGAACTTCTCTCGTGTCAACAAAACTCCTTTTGAGAACAGAAGTCCTGTTTCTATTCCTGCTCCATCTCCAATGCAAAAGAAAAAGAAACTTGCTGATCTCTTTCGCGAGAGCTTCAGAGATGGTGATGATGTTGATGATGCAACAGATCACTCAGGTGATCAAACCATGACTAATGGAAAGATGGAAGTGAAGCCCACCACCCAAGACCTCCCTCCAAAATCTCTGAATGGTACTCCTAATGTTTCTGGTAGCGAAAGGACAGCCAATGAATATGTAACTGAAGACAGACCAATTAAATCTACACAATGCTGCCTTCCTACCTTTGTTTCTTTTCGCAGCTCTAGTCAGAGGGGGAAGATGAGCCCTACCATTGCTGTAGTTGACAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

235

Amino Acids

25.71

Weight (kDa)

6.33

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 231, 293
AccI GTMKAC 1 cut(s) 122
AccII CGCG 1 cut(s) 420
AcoI YGGCCR 2 cut(s) 39, 133
AcuI CTGAAG 2 cut(s) 412, 615
AfaI GTAC 2 cut(s) 7, 547
AfiI CCNNNNNNNGG 2 cut(s) 231, 293
AflIII ACRYGT 1 cut(s) 8
AleI CACNNNNGTG 1 cut(s) 468
AluBI AGCT 4 cut(s) 82, 426, 654, 704
AluI AGCT 4 cut(s) 82, 426, 654, 704
Alw26I GTCTC 1 cut(s) 183
AoxI GGCC 3 cut(s) 39, 133, 210
ApeKI GCWGC 2 cut(s) 624, 651
AspS9I GGNCC 2 cut(s) 66, 307
AsuC2I CCSGG 2 cut(s) 34, 104
AsuHPI GGTGA 3 cut(s) 287, 449, 482
AvaII GGWCC 2 cut(s) 66, 307
BanII GRGCYC 1 cut(s) 680
BarI GAAGNNNNNNTAC 1 cut(s) 30
BauI CACGAG 2 cut(s) 291, 324
BbsI GAAGAC 1 cut(s) 603
BbvI GCAGC 2 cut(s) 611, 663
BccI CCATC 5 cut(s) 295, 318, 385, 428, 490
BceAI ACGGC 2 cut(s) 120, 195
BclI TGATCA 1 cut(s) 472
BcnI CCSGG 2 cut(s) 34, 104
BcoDI GTCTC 1 cut(s) 183
BfaI CTAG 1 cut(s) 657
BfmI CTRYAG 2 cut(s) 262, 690
BisI GCNGC 2 cut(s) 625, 652
BlsI GCNGC 2 cut(s) 626, 653
Bme1390I CCNGG 2 cut(s) 34, 104
Bme18I GGWCC 2 cut(s) 66, 307
BmgT120I GGNCC 2 cut(s) 66, 307
BmiI GGNNCC 1 cut(s) 67
BmrFI CCNGG 2 cut(s) 34, 104
BmsI GCATC 1 cut(s) 442
BpiI GAAGAC 1 cut(s) 603
BpuMI CCSGG 2 cut(s) 34, 104
BsaAI YACGTR 1 cut(s) 9
BsaJI CCNNGG 3 cut(s) 102, 177, 230
BsaWI WCCGGW 2 cut(s) 73, 304
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 2 cut(s) 231, 293
Bse3DI GCAATG 1 cut(s) 684
BseDI CCNNGG 3 cut(s) 102, 177, 230
BseLI CCNNNNNNNGG 2 cut(s) 231, 293
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 2 cut(s) 255, 480
BseRI GAGGAG 1 cut(s) 76
BseXI GCAGC 2 cut(s) 611, 663
Bsh1236I CGCG 1 cut(s) 420
BshFI GGCC 3 cut(s) 41, 135, 212
BsiSI CCGG 4 cut(s) 33, 74, 104, 305
BslFI GGGAC 1 cut(s) 85
BslI CCNNNNNNNGG 2 cut(s) 231, 293
BsmAI GTCTC 1 cut(s) 183
BsmFI GGGAC 1 cut(s) 85
BsnI GGCC 3 cut(s) 41, 135, 212
Bsp1286I GDGCHC 1 cut(s) 680
Bsp143I GATC 4 cut(s) 141, 409, 460, 472
Bsp68I TCGCGA 1 cut(s) 420
BspANI GGCC 3 cut(s) 41, 135, 212
BspCNI CTCAG 2 cut(s) 254, 479
BspFNI CGCG 1 cut(s) 420
BspLI GGNNCC 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 684
BssECI CCNNGG 3 cut(s) 102, 177, 230
BssMI GATC 4 cut(s) 141, 409, 460, 472
BssSI CACGAG 2 cut(s) 291, 324
BssT1I CCWWGG 1 cut(s) 230
Bst2BI CACGAG 2 cut(s) 291, 324
Bst4CI ACNGT 5 cut(s) 99, 120, 131, 197, 266
BstBAI YACGTR 1 cut(s) 9
BstC8I GCNNGC 2 cut(s) 162, 166
BstDEI CTNAG 2 cut(s) 241, 466
BstDSI CCRYGG 1 cut(s) 177
BstFNI CGCG 1 cut(s) 420
BstKTI GATC 4 cut(s) 144, 412, 463, 475
BstMAI GTCTC 1 cut(s) 183
BstMBI GATC 4 cut(s) 141, 409, 460, 472
BstSCI CCNGG 2 cut(s) 32, 102
BstSFI CTRYAG 2 cut(s) 262, 690
BstUI CGCG 1 cut(s) 420
BstV1I GCAGC 2 cut(s) 611, 663
BstV2I GAAGAC 1 cut(s) 603
BsuRI GGCC 3 cut(s) 41, 135, 212
BtgI CCRYGG 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 271
BtuMI TCGCGA 1 cut(s) 420
Cac8I GCNNGC 2 cut(s) 162, 166
Cfr13I GGNCC 2 cut(s) 66, 307
Csp6I GTAC 2 cut(s) 6, 546
CviAII CATG 2 cut(s) 43, 481
CviQI GTAC 2 cut(s) 6, 546
DdeI CTNAG 2 cut(s) 241, 466
DpnI GATC 4 cut(s) 143, 411, 462, 474
DpnII GATC 4 cut(s) 141, 409, 460, 472
EaeI YGGCCR 2 cut(s) 39, 133
Eco130I CCWWGG 1 cut(s) 230
Eco147I AGGCCT 1 cut(s) 212
Eco24I GRGCYC 1 cut(s) 680
Eco47I GGWCC 2 cut(s) 66, 307
Eco57I CTGAAG 2 cut(s) 412, 615
EcoT14I CCWWGG 1 cut(s) 230
EcoT38I GRGCYC 1 cut(s) 680
ErhI CCWWGG 1 cut(s) 230
FaeI CATG 2 cut(s) 46, 484
FaiI YATR 4 cut(s) 24, 44, 482, 588
FaqI GGGAC 1 cut(s) 85
FatI CATG 2 cut(s) 42, 480
FbaI TGATCA 1 cut(s) 472
FblI GTMKAC 1 cut(s) 122
Fnu4HI GCNGC 2 cut(s) 625, 652
FriOI GRGCYC 1 cut(s) 680
Fsp4HI GCNGC 2 cut(s) 625, 652
FspBI CTAG 1 cut(s) 657
GluI GCNGC 2 cut(s) 625, 652
HaeIII GGCC 3 cut(s) 41, 135, 212
HapII CCGG 4 cut(s) 33, 74, 104, 305
Hin1II CATG 2 cut(s) 46, 484
HincII GTYRAC 3 cut(s) 123, 331, 697
HindII GTYRAC 3 cut(s) 123, 331, 697
HindIII AAGCTT 1 cut(s) 702
HinfI GANTC 3 cut(s) 29, 221, 239
HpaII CCGG 4 cut(s) 33, 74, 104, 305
HphI GGTGA 3 cut(s) 287, 449, 482
Hpy166II GTNNAC 3 cut(s) 123, 331, 697
Hpy188I TCNGA 8 cut(s) 53, 141, 188, 244, 315, 431, 540, 663
Hpy188III TCNNGA 1 cut(s) 419
Hpy8I GTNNAC 3 cut(s) 123, 331, 697
Hpy99I CGWCG 1 cut(s) 124
HpyAV CCTTC 2 cut(s) 223, 638
HpyCH4III ACNGT 5 cut(s) 99, 120, 131, 197, 266
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 2 cut(s) 388, 455
HpyF3I CTNAG 2 cut(s) 241, 466
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 2 cut(s) 46, 484
Ksp22I TGATCA 1 cut(s) 472
Kzo9I GATC 4 cut(s) 141, 409, 460, 472
LmnI GCTCC 1 cut(s) 379
LpnPI CCDG 9 cut(s) 46, 87, 117, 174, 318, 372, 384, 453, 546
Lsp1109I GCAGC 2 cut(s) 611, 663
LweI GCATC 1 cut(s) 442
MaeI CTAG 1 cut(s) 657
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 2 cut(s) 275, 589
MalI GATC 4 cut(s) 143, 411, 462, 474
MboI GATC 4 cut(s) 141, 409, 460, 472
MboII GAAGA 3 cut(s) 263, 608, 682
MhlI GDGCHC 1 cut(s) 680
MluCI AATT 1 cut(s) 606
MlyI GAGTC 1 cut(s) 233
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 5 cut(s) 97, 202, 533, 537, 657
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 1 cut(s) 468
MspI CCGG 4 cut(s) 33, 74, 104, 305
MspR9I CCNGG 2 cut(s) 34, 104
MvnI CGCG 1 cut(s) 420
NciI CCSGG 2 cut(s) 34, 104
NdeII GATC 4 cut(s) 141, 409, 460, 472
NlaIII CATG 2 cut(s) 46, 484
NlaIV GGNNCC 1 cut(s) 67
NmuCI GTSAC 1 cut(s) 275
NruI TCGCGA 1 cut(s) 420
OliI CACNNNNGTG 1 cut(s) 468
PceI AGGCCT 1 cut(s) 212
PfeI GAWTC 2 cut(s) 29, 221
PflMI CCANNNNNTGG 2 cut(s) 231, 293
PkrI GCNGC 2 cut(s) 626, 653
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
Ppu21I YACGTR 1 cut(s) 9
PspN4I GGNNCC 1 cut(s) 67
PspPI GGNCC 2 cut(s) 66, 307
RruI TCGCGA 1 cut(s) 420
RsaI GTAC 2 cut(s) 7, 547
RsaNI GTAC 2 cut(s) 6, 546
RseI CAYNNNNRTG 1 cut(s) 468
SalI GTCGAC 1 cut(s) 121
SaqAI TTAA 1 cut(s) 609
SatI GCNGC 2 cut(s) 625, 652
Sau3AI GATC 4 cut(s) 141, 409, 460, 472
Sau96I GGNCC 2 cut(s) 66, 307
SchI GAGTC 1 cut(s) 233
ScrFI CCNGG 2 cut(s) 34, 104
SduI GDGCHC 1 cut(s) 680
SetI ASST 8 cut(s) 11, 84, 428, 472, 525, 638, 656, 706
SfaNI GCATC 1 cut(s) 442
SfcI CTRYAG 2 cut(s) 262, 690
SinI GGWCC 2 cut(s) 66, 307
SmiMI CAYNNNNRTG 1 cut(s) 468
Sse9I AATT 1 cut(s) 606
SseBI AGGCCT 1 cut(s) 212
SspMI CTAG 1 cut(s) 657
StuI AGGCCT 1 cut(s) 212
StyD4I CCNGG 2 cut(s) 32, 102
StyI CCWWGG 1 cut(s) 230
TaaI ACNGT 5 cut(s) 99, 120, 131, 197, 266
TaiI ACGT 1 cut(s) 11
TaqI TCGA 1 cut(s) 122
TasI AATT 1 cut(s) 606
TfiI GAWTC 2 cut(s) 29, 221
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 1 cut(s) 275
TseI GCWGC 2 cut(s) 624, 651
Tsp45I GTSAC 1 cut(s) 275
TspDTI ATGAA 3 cut(s) 59, 165, 597
TspRI CASTG 1 cut(s) 271
Van91I CCANNNNNTGG 2 cut(s) 231, 293
VpaK11BI GGWCC 2 cut(s) 66, 307
XcmI CCANNNNNNNNNTGG 1 cut(s) 232
XmiI GTMKAC 1 cut(s) 122
XspI CTAG 1 cut(s) 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.