Rorug02G0581900

At3g27210-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008381
Physical Location & Seq
Forward (+)
70568108 .. 70569651
1544 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0581900.1

Sequence Viewer

Length: 705 bp
ATGGCGGTTTCTAGGAATTTTTGTATCTCTGCGGCATTGGGTTCTTGTCCTTTTAAGCACCACTGTCTGGGAAGCTCTTTCCAACCTTCTAAGGTAACATTTAAGGGTTGTTATGGTGTAGTATTTAGTAATTCATGGGAGATGAGTCAGATGACACAACCATCCTGCTTGAATTCGAAAGGGTACAAGTCTAAGTGCCACTATAACCAATCATTCGGGATTGCAGAAGACAGTGAACTTATTTCCGAACCTGTAAGCCAAGAAACAGAAGGCTTTCTCCTTGACGCTATCAATATGAGCTTCTTCGAGCGTTTAAACTTAGCATGGAAGATAATGTTCCCTTCACCATCTACTAGAAGAAATTCCAATGCAAAGATTGCCAAGCAACGCTTGCAGATGATCCTGTTCTCTGATCGATGTGCTGTAACTGATGGTGCAAAGCAGAAGATTGTGAGCAACGTTGTGGGCGCTCTCTCTGACTTTGTAGAGATAGACTCACAGGACAAAGTCCAGCTTAGCGTCTCAGCAGATTCAGAAACGGGTACAGTGTACTCTGTTACAGTTCCTGTTCGGCGCGTGAAGCCTGAACATCAAGCTGTGGATGAGCTTGGTGCCTTGAAAAATGTTGAGTACAAGGATACAGGGACATCATCAGATTCTGTTGATGTTAAGTTTGATTTGTACATCCCTAACAAGTCTCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

234

Amino Acids

25.84

Weight (kDa)

6.89

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MinE PF03776 124 - 191 9.9e-13 Septum formation topological specificity factor MinE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 611
AccB7I CCANNNNNTGG 1 cut(s) 67
AccII CGCG 1 cut(s) 576
AciI CCGC 2 cut(s) 5, 32
AclI AACGTT 1 cut(s) 459
AclWI GGATC 1 cut(s) 394
AcsI RAATTY 3 cut(s) 16, 172, 361
AfaI GTAC 5 cut(s) 185, 544, 551, 632, 683
AfiI CCNNNNNNNGG 1 cut(s) 67
AgsI TTSAA 2 cut(s) 172, 619
AluBI AGCT 5 cut(s) 75, 300, 514, 596, 607
AluI AGCT 5 cut(s) 75, 300, 514, 596, 607
Alw26I GTCTC 1 cut(s) 526
AlwI GGATC 1 cut(s) 394
AlwNI CAGNNNCTG 2 cut(s) 566, 659
ApoI RAATTY 3 cut(s) 16, 172, 361
Asp700I GAANNNNTTC 2 cut(s) 273, 361
AspLEI GCGC 2 cut(s) 470, 576
AsuHPI GGTGA 1 cut(s) 336
AsuII TTCGAA 1 cut(s) 176
BanI GGYRCC 1 cut(s) 611
BbsI GAAGAC 1 cut(s) 234
BccI CCATC 3 cut(s) 169, 355, 425
BciVI GTATCC 1 cut(s) 631
BcoDI GTCTC 1 cut(s) 526
BfaI CTAG 2 cut(s) 12, 354
BfoI RGCGCY 1 cut(s) 471
BfuI GTATCC 1 cut(s) 631
BisI GCNGC 1 cut(s) 33
BlpI GCTNAGC 1 cut(s) 515
BlsI GCNGC 1 cut(s) 34
BmiI GGNNCC 1 cut(s) 613
BpiI GAAGAC 1 cut(s) 234
BplI GAGNNNNNCTC 2 cut(s) 479, 511
Bpu1102I GCTNAGC 1 cut(s) 515
Bpu14I TTCGAA 1 cut(s) 176
Bsa29I ATCGAT 1 cut(s) 415
Bsc4I CCNNNNNNNGG 1 cut(s) 67
BseCI ATCGAT 1 cut(s) 415
BseGI GGATG 3 cut(s) 161, 607, 684
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMII CTCAG 1 cut(s) 537
Bsh1236I CGCG 1 cut(s) 576
BshNI GGYRCC 1 cut(s) 611
BshVI ATCGAT 1 cut(s) 415
BslFI GGGAC 1 cut(s) 658
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 1 cut(s) 526
BsmBI CGTCTC 1 cut(s) 526
BsmFI GGGAC 1 cut(s) 658
Bsp119I TTCGAA 1 cut(s) 176
Bsp1407I TGTACA 1 cut(s) 681
Bsp143I GATC 2 cut(s) 399, 412
Bsp1720I GCTNAGC 1 cut(s) 515
BspACI CCGC 2 cut(s) 5, 32
BspCNI CTCAG 1 cut(s) 536
BspDI ATCGAT 1 cut(s) 415
BspFNI CGCG 1 cut(s) 576
BspLI GGNNCC 1 cut(s) 613
BspPI GGATC 1 cut(s) 394
BspT104I TTCGAA 1 cut(s) 176
BspT107I GGYRCC 1 cut(s) 611
BsrGI TGTACA 1 cut(s) 681
BssMI GATC 2 cut(s) 399, 412
Bst4CI ACNGT 4 cut(s) 65, 233, 547, 562
BstAPI GCANNNNNTGC 2 cut(s) 377, 391
BstAUI TGTACA 1 cut(s) 681
BstBI TTCGAA 1 cut(s) 176
BstC8I GCNNGC 1 cut(s) 392
BstDEI CTNAG 5 cut(s) 90, 192, 319, 515, 523
BstF5I GGATG 3 cut(s) 161, 607, 684
BstFNI CGCG 1 cut(s) 576
BstH2I RGCGCY 1 cut(s) 471
BstHHI GCGC 2 cut(s) 470, 576
BstKTI GATC 2 cut(s) 402, 415
BstMAI GTCTC 1 cut(s) 526
BstMBI GATC 2 cut(s) 399, 412
BstMWI GCNNNNNNNGC 3 cut(s) 377, 391, 580
BstUI CGCG 1 cut(s) 576
BstV2I GAAGAC 1 cut(s) 234
Bsu15I ATCGAT 1 cut(s) 415
BsuI GTATCC 1 cut(s) 631
BsuTUI ATCGAT 1 cut(s) 415
BtsCI GGATG 3 cut(s) 161, 607, 684
BtsIMutI CAGTG 3 cut(s) 61, 238, 552
Cac8I GCNNGC 1 cut(s) 392
CaiI CAGNNNCTG 2 cut(s) 566, 659
CfoI GCGC 2 cut(s) 470, 576
ClaI ATCGAT 1 cut(s) 415
CseI GACGC 2 cut(s) 293, 508
Csp6I GTAC 5 cut(s) 184, 543, 550, 631, 682
CviAII CATG 2 cut(s) 135, 324
CviJI RGCY 8 cut(s) 75, 258, 273, 300, 514, 583, 596, 607
CviKI_1 RGCY 8 cut(s) 75, 258, 273, 300, 514, 583, 596, 607
CviQI GTAC 5 cut(s) 184, 543, 550, 631, 682
DdeI CTNAG 5 cut(s) 90, 192, 319, 515, 523
DpnI GATC 2 cut(s) 401, 414
DpnII GATC 2 cut(s) 399, 412
DraI TTTAAA 1 cut(s) 315
EcoRI GAATTC 1 cut(s) 172
Esp3I CGTCTC 1 cut(s) 526
FaeI CATG 2 cut(s) 138, 327
FaiI YATR 5 cut(s) 114, 136, 204, 296, 325
FalI AAGNNNNNCTT 4 cut(s) 374, 406, 498, 530
FaqI GGGAC 1 cut(s) 658
FatI CATG 2 cut(s) 134, 323
Fnu4HI GCNGC 1 cut(s) 33
FokI GGATG 3 cut(s) 148, 614, 671
Fsp4HI GCNGC 1 cut(s) 33
FspBI CTAG 2 cut(s) 12, 354
GlaI GCGC 2 cut(s) 469, 575
GluI GCNGC 1 cut(s) 33
HaeII RGCGCY 1 cut(s) 471
HgaI GACGC 2 cut(s) 293, 508
HhaI GCGC 2 cut(s) 470, 576
Hin1II CATG 2 cut(s) 138, 327
Hin6I GCGC 2 cut(s) 468, 574
HinP1I GCGC 2 cut(s) 468, 574
HinfI GANTC 4 cut(s) 145, 494, 530, 656
HphI GGTGA 1 cut(s) 336
Hpy166II GTNNAC 2 cut(s) 236, 550
Hpy188I TCNGA 6 cut(s) 150, 247, 412, 478, 535, 655
Hpy188III TCNNGA 1 cut(s) 217
Hpy8I GTNNAC 2 cut(s) 236, 550
HpyAV CCTTC 3 cut(s) 96, 263, 351
HpyCH4III ACNGT 4 cut(s) 65, 233, 547, 562
HpyCH4IV ACGT 1 cut(s) 459
HpyCH4V TGCA 4 cut(s) 224, 371, 394, 437
HpyF10VI GCNNNNNNNGC 3 cut(s) 377, 391, 580
HpyF3I CTNAG 5 cut(s) 90, 192, 319, 515, 523
HpySE526I ACGT 1 cut(s) 459
Hsp92II CATG 2 cut(s) 138, 327
HspAI GCGC 2 cut(s) 468, 574
Kzo9I GATC 2 cut(s) 399, 412
LpnPI CCDG 9 cut(s) 53, 178, 264, 416, 485, 524, 579, 597, 627
MaeI CTAG 2 cut(s) 12, 354
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 3 cut(s) 94, 424, 556
MalI GATC 2 cut(s) 401, 414
MboI GATC 2 cut(s) 399, 412
MboII GAAGA 5 cut(s) 239, 295, 340, 369, 457
MluCI AATT 4 cut(s) 16, 130, 172, 361
MlyI GAGTC 2 cut(s) 154, 488
MmeI TCCRAC 1 cut(s) 106
MroXI GAANNNNTTC 2 cut(s) 273, 361
MseI TTAA 4 cut(s) 54, 102, 314, 669
MssI GTTTAAAC 1 cut(s) 315
MvnI CGCG 1 cut(s) 576
MwoI GCNNNNNNNGC 3 cut(s) 377, 391, 580
NdeII GATC 2 cut(s) 399, 412
NlaIII CATG 2 cut(s) 138, 327
NlaIV GGNNCC 1 cut(s) 613
NspV TTCGAA 1 cut(s) 176
PdmI GAANNNNTTC 2 cut(s) 273, 361
PfeI GAWTC 2 cut(s) 530, 656
PflFI GACNNNGTC 1 cut(s) 506
PflMI CCANNNNNTGG 1 cut(s) 67
PkrI GCNGC 1 cut(s) 34
PleI GAGTC 2 cut(s) 153, 488
PmeI GTTTAAAC 1 cut(s) 315
PpsI GAGTC 2 cut(s) 153, 488
Psp1406I AACGTT 1 cut(s) 459
PspN4I GGNNCC 1 cut(s) 613
PstNI CAGNNNCTG 2 cut(s) 566, 659
PsyI GACNNNGTC 1 cut(s) 506
RsaI GTAC 5 cut(s) 185, 544, 551, 632, 683
RsaNI GTAC 5 cut(s) 184, 543, 550, 631, 682
SaqAI TTAA 4 cut(s) 54, 102, 314, 669
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 2 cut(s) 399, 412
SchI GAGTC 2 cut(s) 154, 488
SetI ASST 9 cut(s) 77, 88, 96, 253, 302, 462, 516, 598, 609
SfuI TTCGAA 1 cut(s) 176
Sse9I AATT 4 cut(s) 16, 130, 172, 361
SsiI CCGC 2 cut(s) 5, 32
SspMI CTAG 2 cut(s) 12, 354
TaaI ACNGT 4 cut(s) 65, 233, 547, 562
TaiI ACGT 1 cut(s) 462
TaqI TCGA 3 cut(s) 176, 306, 415
TasI AATT 4 cut(s) 16, 130, 172, 361
TatI WGTACW 3 cut(s) 549, 630, 681
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 2 cut(s) 530, 656
Tru1I TTAA 4 cut(s) 54, 102, 314, 669
Tru9I TTAA 4 cut(s) 54, 102, 314, 669
TscAI CASTG 3 cut(s) 68, 238, 552
TspDTI ATGAA 1 cut(s) 123
TspGWI ACGGA 1 cut(s) 690
TspRI CASTG 3 cut(s) 68, 238, 552
Tth111I GACNNNGTC 1 cut(s) 506
Van91I CCANNNNNTGG 1 cut(s) 67
XapI RAATTY 3 cut(s) 16, 172, 361
XmnI GAANNNNTTC 2 cut(s) 273, 361
XspI CTAG 2 cut(s) 12, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.