RchiOBHm_Chr3g0451731

Belongs to the SKP1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0005222
Physical Location & Seq
Reverse (-)
2685309 .. 2685671
363 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41896

Sequence Viewer

Length: 363 bp
ATGTCGTCCGGAAGGAAGATAACGCTGAAGAGCTCCGACGGCGAGTCGTTCGAGGTGGACGAGGAGGTGGCTTTGGAATCGCAGACCATCCAGCACATGATCGAGGACGATTGCGCCGACATTGAGATCCCTTTCCCCAGCGAGATCTTGGCTAAGGTCAGCGAATACTGCAAGAAGCACGGCGACGCTGCGCCTAAGTCCAACAACCGCTCCTCCTCCTCCGGCGACGATGAGCTCAAGAACTGGGACGCCGACTTCGTCAAGGTCGACGAGGTCACCTTCTTCTATCTCCAATTGGTCCGTATTACTACCTTTAGCCTATTACTGCTGCTTTCTCTTTTATCGCTGCTTCAATTGGCTTAG

Protein Analysis

120

Amino Acids

13.4

Weight (kDa)

4.42

Isoelectric Point (pI)

54.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Skp1_POZ PF03931 6 - 60 3.4e-22 Skp1 family, tetramerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 210
AccI GTMKAC 1 cut(s) 267
AccIII TCCGGA 1 cut(s) 8
AciI CCGC 1 cut(s) 208
AclWI GGATC 1 cut(s) 121
AcuI CTGAAG 1 cut(s) 47
AcyI GRCGYC 1 cut(s) 249
AgsI TTSAA 1 cut(s) 353
AhdI GACNNNNNGTC 1 cut(s) 43
AluBI AGCT 2 cut(s) 33, 235
AluI AGCT 2 cut(s) 33, 235
Alw21I GWGCWC 2 cut(s) 35, 237
AlwI GGATC 1 cut(s) 121
Aor13HI TCCGGA 1 cut(s) 8
ApeKI GCWGC 3 cut(s) 188, 328, 346
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
AspLEI GCGC 2 cut(s) 116, 193
AspS9I GGNCC 1 cut(s) 298
AsuHPI GGTGA 1 cut(s) 268
AvaII GGWCC 1 cut(s) 298
BanII GRGCYC 2 cut(s) 35, 237
Bbv12I GWGCWC 2 cut(s) 35, 237
BbvI GCAGC 3 cut(s) 175, 315, 333
BccI CCATC 1 cut(s) 95
BceAI ACGGC 2 cut(s) 55, 196
BglII AGATCT 1 cut(s) 144
BisI GCNGC 3 cut(s) 189, 329, 347
BlsI GCNGC 3 cut(s) 190, 330, 348
Bme18I GGWCC 1 cut(s) 298
BmeRI GACNNNNNGTC 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 298
BmrI ACTGGG 1 cut(s) 253
BmuI ACTGGG 1 cut(s) 253
Bpu10I CCTNAGC 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 221
BsaHI GRCGYC 1 cut(s) 249
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 4 cut(s) 194, 197, 224, 227
Bse1I ACTGG 1 cut(s) 248
BseAI TCCGGA 1 cut(s) 8
BseGI GGATG 1 cut(s) 87
BseNI ACTGG 1 cut(s) 248
BseRI GAGGAG 4 cut(s) 77, 202, 205, 208
BseXI GCAGC 3 cut(s) 175, 315, 333
BseYI CCCAGC 1 cut(s) 137
BsiHKAI GWGCWC 2 cut(s) 35, 237
BsiSI CCGG 2 cut(s) 9, 222
BslFI GGGAC 1 cut(s) 260
BsmFI GGGAC 1 cut(s) 260
Bsp1286I GDGCHC 2 cut(s) 35, 237
Bsp13I TCCGGA 1 cut(s) 8
Bsp143I GATC 3 cut(s) 99, 126, 144
BspACI CCGC 1 cut(s) 208
BspEI TCCGGA 1 cut(s) 8
BspPI GGATC 1 cut(s) 121
BspQI GCTCTTC 1 cut(s) 23
BsrBI CCGCTC 1 cut(s) 210
BsrI ACTGG 1 cut(s) 248
BssMI GATC 3 cut(s) 99, 126, 144
BssNI GRCGYC 1 cut(s) 249
Bst6I CTCTTC 1 cut(s) 23
BstACI GRCGYC 1 cut(s) 249
BstDEI CTNAG 3 cut(s) 153, 195, 360
BstEII GGTNACC 1 cut(s) 274
BstF5I GGATG 1 cut(s) 87
BstHHI GCGC 2 cut(s) 116, 193
BstKTI GATC 3 cut(s) 102, 129, 147
BstMBI GATC 3 cut(s) 99, 126, 144
BstMWI GCNNNNNNNGC 2 cut(s) 39, 168
BstPI GGTNACC 1 cut(s) 274
BstV1I GCAGC 3 cut(s) 175, 315, 333
BstX2I RGATCY 2 cut(s) 126, 144
BstYI RGATCY 2 cut(s) 126, 144
BtsCI GGATG 1 cut(s) 87
CfoI GCGC 2 cut(s) 116, 193
Cfr13I GGNCC 1 cut(s) 298
CseI GACGC 2 cut(s) 194, 257
CviAII CATG 1 cut(s) 97
CviJI RGCY 6 cut(s) 33, 71, 152, 235, 318, 359
CviKI_1 RGCY 6 cut(s) 33, 71, 152, 235, 318, 359
DdeI CTNAG 3 cut(s) 153, 195, 360
DpnI GATC 3 cut(s) 101, 128, 146
DpnII GATC 3 cut(s) 99, 126, 144
DriI GACNNNNNGTC 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 23
Eam1105I GACNNNNNGTC 1 cut(s) 43
EarI CTCTTC 1 cut(s) 23
Ecl136II GAGCTC 2 cut(s) 33, 235
Eco24I GRGCYC 2 cut(s) 35, 237
Eco47I GGWCC 1 cut(s) 298
Eco53kI GAGCTC 2 cut(s) 33, 235
Eco57I CTGAAG 1 cut(s) 47
Eco91I GGTNACC 1 cut(s) 274
EcoICRI GAGCTC 2 cut(s) 33, 235
EcoO65I GGTNACC 1 cut(s) 274
EcoT38I GRGCYC 2 cut(s) 35, 237
FaeI CATG 1 cut(s) 100
FaiI YATR 1 cut(s) 98
FaqI GGGAC 1 cut(s) 260
FatI CATG 1 cut(s) 96
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 3 cut(s) 189, 329, 347
FokI GGATG 1 cut(s) 74
FriOI GRGCYC 2 cut(s) 35, 237
Fsp4HI GCNGC 3 cut(s) 189, 329, 347
GlaI GCGC 2 cut(s) 115, 192
GluI GCNGC 3 cut(s) 189, 329, 347
GsaI CCCAGC 1 cut(s) 141
HapII CCGG 2 cut(s) 9, 222
HgaI GACGC 2 cut(s) 194, 257
HhaI GCGC 2 cut(s) 116, 193
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 1 cut(s) 100
Hin6I GCGC 2 cut(s) 114, 191
HinP1I GCGC 2 cut(s) 114, 191
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 2 cut(s) 44, 77
HpaII CCGG 2 cut(s) 9, 222
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 2 cut(s) 58, 268
Hpy188I TCNGA 1 cut(s) 37
Hpy188III TCNNGA 2 cut(s) 9, 238
Hpy8I GTNNAC 2 cut(s) 58, 268
Hpy99I CGWCG 4 cut(s) 41, 188, 230, 272
HpyAV CCTTC 2 cut(s) 6, 289
HpyCH4V TGCA 1 cut(s) 171
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 168
HpyF3I CTNAG 3 cut(s) 153, 195, 360
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 1 cut(s) 100
HspAI GCGC 2 cut(s) 114, 191
Kpn2I TCCGGA 1 cut(s) 8
Kzo9I GATC 3 cut(s) 99, 126, 144
LguI GCTCTTC 1 cut(s) 23
LmnI GCTCC 2 cut(s) 38, 215
LpnPI CCDG 5 cut(s) 22, 104, 151, 229, 235
Lsp1109I GCAGC 3 cut(s) 175, 315, 333
MaeIII GTNAC 1 cut(s) 274
MalI GATC 3 cut(s) 101, 128, 146
MbiI CCGCTC 1 cut(s) 210
MboI GATC 3 cut(s) 99, 126, 144
MboII GAAGA 3 cut(s) 28, 40, 274
MfeI CAATTG 2 cut(s) 293, 353
MflI RGATCY 2 cut(s) 126, 144
MhlI GDGCHC 2 cut(s) 35, 237
MluCI AATT 2 cut(s) 293, 353
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 2 cut(s) 60, 225
MnlI CCTC 8 cut(s) 46, 55, 58, 97, 223, 226, 229, 265
MroI TCCGGA 1 cut(s) 8
MspI CCGG 2 cut(s) 9, 222
MunI CAATTG 2 cut(s) 293, 353
MwoI GCNNNNNNNGC 2 cut(s) 39, 168
NdeII GATC 3 cut(s) 99, 126, 144
NlaIII CATG 1 cut(s) 100
NmuCI GTSAC 1 cut(s) 274
PciSI GCTCTTC 1 cut(s) 23
PcsI WCGNNNNNNNCGW 4 cut(s) 57, 114, 255, 264
PfeI GAWTC 1 cut(s) 77
PflFI GACNNNGTC 2 cut(s) 257, 272
PkrI GCNGC 3 cut(s) 190, 330, 348
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
Psp124BI GAGCTC 2 cut(s) 35, 237
PspEI GGTNACC 1 cut(s) 274
PspFI CCCAGC 1 cut(s) 137
PspPI GGNCC 1 cut(s) 298
PsuI RGATCY 2 cut(s) 126, 144
PsyI GACNNNGTC 2 cut(s) 257, 272
SacI GAGCTC 2 cut(s) 35, 237
SalI GTCGAC 1 cut(s) 266
SapI GCTCTTC 1 cut(s) 23
SatI GCNGC 3 cut(s) 189, 329, 347
Sau3AI GATC 3 cut(s) 99, 126, 144
Sau96I GGNCC 1 cut(s) 298
SchI GAGTC 1 cut(s) 53
SduI GDGCHC 2 cut(s) 35, 237
SetI ASST 9 cut(s) 35, 57, 69, 159, 237, 267, 276, 281, 314
SinI GGWCC 1 cut(s) 298
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
Sse9I AATT 2 cut(s) 293, 353
SsiI CCGC 1 cut(s) 208
SstI GAGCTC 2 cut(s) 35, 237
TaqI TCGA 3 cut(s) 51, 102, 267
TasI AATT 2 cut(s) 293, 353
TfiI GAWTC 1 cut(s) 77
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 3 cut(s) 188, 328, 346
Tsp45I GTSAC 1 cut(s) 274
TspGWI ACGGA 1 cut(s) 290
Tth111I GACNNNGTC 2 cut(s) 257, 272
VpaK11BI GGWCC 1 cut(s) 298
XcmI CCANNNNNNNNNTGG 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.