RchiOBHm_Chr3g0485001

N-lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0003800
Physical Location & Seq
Reverse (-)
31584506 .. 31585842
1337 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44967

Sequence Viewer

Length: 846 bp
ATGGAAATGGCGGCTCGGCGCGTGAGGGCTTTCAAGCGCTGGATGAAGCGGGAAGGCATCCAATGCAGCGACGCCCTCGAGCTGACCGTCTCCGCCACCCAAGGCCTCTCCATCACTGCCCTCGCCGACCTCCACGTCGGCGACGCCGTCGCCAGAATCCCCAAGGCCACGTGCCTCACCGTCATCACCACCGCCGCGCGCGACATGATCGACGCGGCCGGCCTCGACGGCAGCCTGGGCCTCGCGGTGGCGATCATGCACGAGAGGAGCCTGGGGGAGCAGTCCCACTGGGCCCCGTACCTGGCGGCGTTGCCGGAGCAGGAGGCCATCCCGTTGGTCTGGACGGCGGAGGAGGCGGAGGTGCTGCTGAGAGGGACGGAGCTGCACGAGACGGTGAAGGAAGACAGAGGAGTGATGCTGGAGGACTGGAAAGACAGCATTGAGCCATTGCAGGATCAAATTGGGTCGACCCAATTTGAGAGCTTTTTCGGGTTTGAGGAGTACTTGGCAGCAAGAACGCTGATTGCTTCTAGGTCTTTTCAGATTGATGATCACCACCACGGAGTCGGAATGGTTCCTTTGGCTGACATCTTTAATCACAAAACTGCAGCTGAGGATGTGCACTTGAACACCTCTGCTTCTACTGAAGGCAGTGAATCCGACACTGATAGTGGTAGCGAAGAAGGCAATGAATCTACTACTAGTACAACTTCCAGTCTTAGTCCTACTACTGGTGCTAGTACTACTGATGATGTAGCTTCTAGTGCTACTGACGATGGTGACGATGATGATTTGGAGATGGTTATGGTCAAAGATGTCAAACTTGGAGCTGAGGTTAGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

281

Amino Acids

30.25

Weight (kDa)

4.41

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 134
AccI GTMKAC 1 cut(s) 467
AccII CGCG 6 cut(s) 21, 197, 199, 201, 215, 245
AclWI GGATC 1 cut(s) 462
AcoI YGGCCR 1 cut(s) 216
AcuI CTGAAG 1 cut(s) 666
AcvI CACGTG 1 cut(s) 171
AcyI GRCGYC 2 cut(s) 72, 144
AfaI GTAC 4 cut(s) 299, 503, 706, 742
AfeI AGCGCT 1 cut(s) 38
AfiI CCNNNNNNNGG 3 cut(s) 247, 301, 731
AgsI TTSAA 2 cut(s) 34, 628
AhlI ACTAGT 1 cut(s) 701
AjiI CACGTC 1 cut(s) 136
AjnI CCWGG 3 cut(s) 234, 270, 300
AluBI AGCT 6 cut(s) 82, 382, 483, 611, 758, 830
AluI AGCT 6 cut(s) 82, 382, 483, 611, 758, 830
Alw21I GWGCWC 1 cut(s) 624
Alw26I GTCTC 2 cut(s) 94, 383
Alw44I GTGCAC 1 cut(s) 620
AlwI GGATC 1 cut(s) 462
Ama87I CYCGRG 1 cut(s) 77
Aor51HI AGCGCT 1 cut(s) 38
AoxI GGCC 7 cut(s) 103, 165, 216, 220, 238, 291, 324
ApaI GGGCCC 1 cut(s) 295
ApaLI GTGCAC 1 cut(s) 620
ApeKI GCWGC 6 cut(s) 66, 231, 364, 382, 509, 608
AspLEI GCGC 4 cut(s) 21, 39, 199, 201
AspS9I GGNCC 3 cut(s) 238, 291, 292
AsuHPI GGTGA 5 cut(s) 169, 178, 406, 545, 791
AvaI CYCGRG 1 cut(s) 77
BaeGI GKGCMC 2 cut(s) 295, 624
BanII GRGCYC 1 cut(s) 295
BauI CACGAG 2 cut(s) 260, 386
BbrPI CACGTG 1 cut(s) 171
BbsI GAAGAC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 624
BbvCI CCTCAGC 2 cut(s) 612, 831
BbvI GCAGC 6 cut(s) 78, 243, 351, 369, 521, 620
BccI CCATC 4 cut(s) 119, 335, 770, 793
BceAI ACGGC 3 cut(s) 131, 244, 360
BciT130I CCWGG 3 cut(s) 236, 272, 302
BclI TGATCA 1 cut(s) 550
BcoDI GTCTC 2 cut(s) 94, 383
BcuI ACTAGT 1 cut(s) 701
BfaI CTAG 5 cut(s) 531, 702, 738, 762, 844
BfmI CTRYAG 1 cut(s) 606
BfoI RGCGCY 1 cut(s) 40
BglI GCCNNNNNGGC 1 cut(s) 228
BmcAI AGTACT 2 cut(s) 503, 742
Bme1390I CCNGG 3 cut(s) 236, 272, 302
BmeT110I CYCGRG 1 cut(s) 77
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 3 cut(s) 238, 291, 292
BmiI GGNNCC 4 cut(s) 269, 293, 294, 576
BmrFI CCNGG 3 cut(s) 236, 272, 302
BmrI ACTGGG 1 cut(s) 298
BmsI GCATC 2 cut(s) 66, 405
BmuI ACTGGG 1 cut(s) 298
BpiI GAAGAC 1 cut(s) 408
BpmI CTGGAG 1 cut(s) 440
Bpu10I CCTNAGC 2 cut(s) 612, 831
BsaAI YACGTR 1 cut(s) 171
BsaHI GRCGYC 2 cut(s) 72, 144
BsaJI CCNNGG 5 cut(s) 100, 162, 235, 271, 559
BsaXI ACNNNNNCTCC 1 cut(s) 819
Bsc4I CCNNNNNNNGG 3 cut(s) 247, 301, 731
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 4 cut(s) 293, 431, 714, 736
Bse3DI GCAATG 2 cut(s) 446, 694
BseBI CCWGG 3 cut(s) 236, 272, 302
BseDI CCNNGG 5 cut(s) 100, 162, 235, 271, 559
BseGI GGATG 4 cut(s) 48, 57, 327, 622
BseLI CCNNNNNNNGG 3 cut(s) 247, 301, 731
BseMI GCAATG 2 cut(s) 446, 694
BseMII CTCAG 3 cut(s) 359, 603, 822
BseNI ACTGG 4 cut(s) 293, 431, 714, 736
BsePI GCGCGC 1 cut(s) 197
BseRI GAGGAG 4 cut(s) 280, 365, 423, 512
BseSI GKGCMC 2 cut(s) 295, 624
BseX3I CGGCCG 1 cut(s) 216
BseXI GCAGC 6 cut(s) 78, 243, 351, 369, 521, 620
BsgI GTGCAG 1 cut(s) 368
Bsh1236I CGCG 6 cut(s) 21, 197, 199, 201, 215, 245
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 7 cut(s) 105, 167, 218, 222, 240, 293, 326
BsiEI CGRYCG 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 624
BsiHKCI CYCGRG 1 cut(s) 77
BsiSI CCGG 2 cut(s) 219, 314
BslFI GGGAC 2 cut(s) 268, 388
BslI CCNNNNNNNGG 3 cut(s) 247, 301, 731
BsmAI GTCTC 2 cut(s) 94, 383
BsmBI CGTCTC 2 cut(s) 94, 383
BsmFI GGGAC 2 cut(s) 268, 388
BsnI GGCC 7 cut(s) 105, 167, 218, 222, 240, 293, 326
BsoBI CYCGRG 1 cut(s) 77
Bsp120I GGGCCC 1 cut(s) 291
Bsp1286I GDGCHC 2 cut(s) 295, 624
Bsp143I GATC 4 cut(s) 207, 252, 454, 550
BspANI GGCC 7 cut(s) 105, 167, 218, 222, 240, 293, 326
BspCNI CTCAG 3 cut(s) 360, 604, 823
BspFNI CGCG 6 cut(s) 21, 197, 199, 201, 215, 245
BspLI GGNNCC 4 cut(s) 269, 293, 294, 576
BspMAI CTGCAG 1 cut(s) 610
BspPI GGATC 1 cut(s) 462
BsrDI GCAATG 2 cut(s) 446, 694
BsrFI RCCGGY 1 cut(s) 218
BsrI ACTGG 4 cut(s) 293, 431, 714, 736
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 5 cut(s) 100, 162, 235, 271, 559
BssHII GCGCGC 1 cut(s) 197
BssMI GATC 4 cut(s) 207, 252, 454, 550
BssNI GRCGYC 2 cut(s) 72, 144
BssSI CACGAG 2 cut(s) 260, 386
BssT1I CCWWGG 2 cut(s) 100, 162
Bst2BI CACGAG 2 cut(s) 260, 386
Bst2UI CCWGG 3 cut(s) 236, 272, 302
Bst4CI ACNGT 3 cut(s) 88, 181, 394
BstACI GRCGYC 2 cut(s) 72, 144
BstAPI GCANNNNNTGC 1 cut(s) 63
BstBAI YACGTR 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 199, 220
BstDEI CTNAG 4 cut(s) 368, 612, 719, 831
BstDSI CCRYGG 1 cut(s) 559
BstF5I GGATG 4 cut(s) 48, 57, 327, 622
BstFNI CGCG 6 cut(s) 21, 197, 199, 201, 215, 245
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 4 cut(s) 21, 39, 199, 201
BstKTI GATC 4 cut(s) 210, 255, 457, 553
BstMAI GTCTC 2 cut(s) 94, 383
BstMBI GATC 4 cut(s) 207, 252, 454, 550
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 6 cut(s) 63, 228, 237, 353, 684, 764
BstNI CCWGG 3 cut(s) 236, 272, 302
BstSCI CCNGG 3 cut(s) 234, 270, 300
BstSFI CTRYAG 1 cut(s) 606
BstSLI GKGCMC 2 cut(s) 295, 624
BstUI CGCG 6 cut(s) 21, 197, 199, 201, 215, 245
BstV1I GCAGC 6 cut(s) 78, 243, 351, 369, 521, 620
BstV2I GAAGAC 1 cut(s) 408
BstXI CCANNNNNNTGG 1 cut(s) 334
BstZI CGGCCG 1 cut(s) 216
BsuRI GGCC 7 cut(s) 105, 167, 218, 222, 240, 293, 326
BtgI CCRYGG 1 cut(s) 559
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 4 cut(s) 48, 57, 327, 622
BtsI GCAGTG 2 cut(s) 114, 658
BtsIMutI CAGTG 4 cut(s) 114, 286, 658, 663
Cac8I GCNNGC 2 cut(s) 199, 220
CfoI GCGC 4 cut(s) 21, 39, 199, 201
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 3 cut(s) 238, 291, 292
CseI GACGC 3 cut(s) 80, 152, 221
Csp6I GTAC 4 cut(s) 298, 502, 705, 741
CviAII CATG 2 cut(s) 205, 256
CviQI GTAC 4 cut(s) 298, 502, 705, 741
DdeI CTNAG 4 cut(s) 368, 612, 719, 831
DpnI GATC 4 cut(s) 209, 254, 456, 552
DpnII GATC 4 cut(s) 207, 252, 454, 550
DrdI GACNNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 1 cut(s) 216
EagI CGGCCG 1 cut(s) 216
EciI GGCGGA 3 cut(s) 82, 362, 371
EclXI CGGCCG 1 cut(s) 216
Eco130I CCWWGG 2 cut(s) 100, 162
Eco147I AGGCCT 1 cut(s) 105
Eco24I GRGCYC 1 cut(s) 295
Eco47III AGCGCT 1 cut(s) 38
Eco52I CGGCCG 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 666
Eco72I CACGTG 1 cut(s) 171
Eco88I CYCGRG 1 cut(s) 77
EcoO109I RGGNCCY 1 cut(s) 292
EcoRII CCWGG 3 cut(s) 234, 270, 300
EcoT14I CCWWGG 2 cut(s) 100, 162
EcoT38I GRGCYC 1 cut(s) 295
ErhI CCWWGG 2 cut(s) 100, 162
Esp3I CGTCTC 2 cut(s) 94, 383
FaeI CATG 2 cut(s) 208, 259
FaiI YATR 3 cut(s) 206, 257, 806
FaqI GGGAC 2 cut(s) 268, 388
FatI CATG 2 cut(s) 204, 255
FauI CCCGC 1 cut(s) 42
FbaI TGATCA 1 cut(s) 550
FblI GTMKAC 1 cut(s) 467
FokI GGATG 4 cut(s) 44, 55, 314, 629
FriOI GRGCYC 1 cut(s) 295
FseI GGCCGGCC 1 cut(s) 222
FspBI CTAG 5 cut(s) 531, 702, 738, 762, 844
GlaI GCGC 4 cut(s) 20, 38, 198, 200
GsuI CTGGAG 1 cut(s) 440
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 7 cut(s) 105, 167, 218, 222, 240, 293, 326
HapII CCGG 2 cut(s) 219, 314
HgaI GACGC 3 cut(s) 80, 152, 221
HhaI GCGC 4 cut(s) 21, 39, 199, 201
Hin1I GRCGYC 2 cut(s) 72, 144
Hin1II CATG 2 cut(s) 208, 259
Hin6I GCGC 4 cut(s) 19, 37, 197, 199
HinP1I GCGC 4 cut(s) 19, 37, 197, 199
HincII GTYRAC 1 cut(s) 468
HindII GTYRAC 1 cut(s) 468
HinfI GANTC 4 cut(s) 156, 564, 656, 692
HpaII CCGG 2 cut(s) 219, 314
HphI GGTGA 5 cut(s) 169, 178, 406, 545, 791
Hpy166II GTNNAC 2 cut(s) 468, 622
Hpy188I TCNGA 3 cut(s) 543, 569, 661
Hpy188III TCNNGA 1 cut(s) 340
Hpy8I GTNNAC 2 cut(s) 468, 622
Hpy99I CGWCG 6 cut(s) 74, 140, 146, 152, 215, 230
HpyAV CCTTC 4 cut(s) 47, 391, 641, 677
HpyCH4III ACNGT 3 cut(s) 88, 181, 394
HpyCH4IV ACGT 2 cut(s) 135, 170
HpyCH4V TGCA 6 cut(s) 66, 259, 385, 451, 608, 622
HpyF10VI GCNNNNNNNGC 6 cut(s) 63, 228, 237, 353, 684, 764
HpyF3I CTNAG 4 cut(s) 368, 612, 719, 831
HpySE526I ACGT 2 cut(s) 135, 170
Hsp92I GRCGYC 2 cut(s) 72, 144
Hsp92II CATG 2 cut(s) 208, 259
HspAI GCGC 4 cut(s) 19, 37, 197, 199
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Ksp22I TGATCA 1 cut(s) 550
Kzo9I GATC 4 cut(s) 207, 252, 454, 550
LmnI GCTCC 5 cut(s) 267, 277, 316, 379, 827
Lsp1109I GCAGC 6 cut(s) 78, 243, 351, 369, 521, 620
LweI GCATC 2 cut(s) 66, 405
MaeI CTAG 5 cut(s) 531, 702, 738, 762, 844
MaeII ACGT 2 cut(s) 135, 170
MaeIII GTNAC 2 cut(s) 779, 839
MalI GATC 4 cut(s) 209, 254, 456, 552
MauBI CGCGCGCG 1 cut(s) 197
MboI GATC 4 cut(s) 207, 252, 454, 550
MboII GAAGA 2 cut(s) 413, 692
MhlI GDGCHC 2 cut(s) 295, 624
MluCI AATT 2 cut(s) 459, 473
MlyI GAGTC 1 cut(s) 573
MmeI TCCRAC 2 cut(s) 547, 684
MroNI GCCGGC 1 cut(s) 218
MseI TTAA 1 cut(s) 594
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 2 cut(s) 219, 314
MspR9I CCNGG 3 cut(s) 236, 272, 302
MvaI CCWGG 3 cut(s) 236, 272, 302
MvnI CGCG 6 cut(s) 21, 197, 199, 201, 215, 245
MwoI GCNNNNNNNGC 6 cut(s) 63, 228, 237, 353, 684, 764
NaeI GCCGGC 1 cut(s) 220
NdeII GATC 4 cut(s) 207, 252, 454, 550
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 2 cut(s) 208, 259
NlaIV GGNNCC 4 cut(s) 269, 293, 294, 576
NmuCI GTSAC 2 cut(s) 779, 839
PaeR7I CTCGAG 1 cut(s) 77
PauI GCGCGC 1 cut(s) 197
PceI AGGCCT 1 cut(s) 105
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 144, 780
PdiI GCCGGC 1 cut(s) 220
PfeI GAWTC 3 cut(s) 156, 656, 692
PflFI GACNNNGTC 1 cut(s) 146
PleI GAGTC 1 cut(s) 572
PmaCI CACGTG 1 cut(s) 171
PmlI CACGTG 1 cut(s) 171
PpsI GAGTC 1 cut(s) 572
Ppu21I YACGTR 1 cut(s) 171
Psp6I CCWGG 3 cut(s) 234, 270, 300
PspCI CACGTG 1 cut(s) 171
PspGI CCWGG 3 cut(s) 234, 270, 300
PspN4I GGNNCC 4 cut(s) 269, 293, 294, 576
PspOMI GGGCCC 1 cut(s) 291
PspPI GGNCC 3 cut(s) 238, 291, 292
PspXI VCTCGAGB 1 cut(s) 77
PstI CTGCAG 1 cut(s) 610
PsyI GACNNNGTC 1 cut(s) 146
PteI GCGCGC 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 611
RigI GGCCGGCC 1 cut(s) 222
RsaI GTAC 4 cut(s) 299, 503, 706, 742
RsaNI GTAC 4 cut(s) 298, 502, 705, 741
SalI GTCGAC 1 cut(s) 466
SaqAI TTAA 1 cut(s) 594
Sau3AI GATC 4 cut(s) 207, 252, 454, 550
Sau96I GGNCC 3 cut(s) 238, 291, 292
ScaI AGTACT 2 cut(s) 503, 742
SchI GAGTC 1 cut(s) 573
ScrFI CCNGG 3 cut(s) 236, 272, 302
SduI GDGCHC 2 cut(s) 295, 624
SfaNI GCATC 2 cut(s) 66, 405
SfcI CTRYAG 1 cut(s) 606
Sfr274I CTCGAG 1 cut(s) 77
SlaI CTCGAG 1 cut(s) 77
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
SpeI ACTAGT 1 cut(s) 701
Sse9I AATT 2 cut(s) 459, 473
SseBI AGGCCT 1 cut(s) 105
SspMI CTAG 5 cut(s) 531, 702, 738, 762, 844
StuI AGGCCT 1 cut(s) 105
StyD4I CCNGG 3 cut(s) 234, 270, 300
StyI CCWWGG 2 cut(s) 100, 162
TaaI ACNGT 3 cut(s) 88, 181, 394
TaiI ACGT 2 cut(s) 138, 173
TaqI TCGA 4 cut(s) 78, 210, 225, 467
TasI AATT 2 cut(s) 459, 473
TatI WGTACW 3 cut(s) 501, 704, 740
TauI GCSGC 4 cut(s) 14, 197, 218, 308
TfiI GAWTC 3 cut(s) 156, 656, 692
Tru1I TTAA 1 cut(s) 594
Tru9I TTAA 1 cut(s) 594
TscAI CASTG 4 cut(s) 121, 293, 658, 670
TseFI GTSAC 2 cut(s) 779, 839
TseI GCWGC 6 cut(s) 66, 231, 364, 382, 509, 608
Tsp45I GTSAC 2 cut(s) 779, 839
TspDTI ATGAA 2 cut(s) 59, 705
TspGWI ACGGA 2 cut(s) 392, 576
TspRI CASTG 4 cut(s) 121, 293, 658, 670
Tth111I GACNNNGTC 1 cut(s) 146
VneI GTGCAC 1 cut(s) 620
XhoI CTCGAG 1 cut(s) 77
XmiI GTMKAC 1 cut(s) 467
XspI CTAG 5 cut(s) 531, 702, 738, 762, 844
ZrmI AGTACT 2 cut(s) 503, 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.