Rorug03G0215100

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003800
Physical Location & Seq
Reverse (-)
19468615 .. 19472927
4313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0215100.1

Sequence Viewer

Length: 897 bp
ATGGGGCTGCTCTCCAACAGAGTGGCTAAGGGAAGCCTTAAACCAGGGGATCACATCTACTCTTGGAGGACTGCTTATATCTATGCCCATCATGGCATCTATATTGGGGATGACAAAGTCATTCATTTCACCAGACGTGGCCAAGAAGTAGGAACTGGGACAGTGTTGGATGTTCTCTTGGTTAGCTCAGGACCAGTCCGACCTCAACAGCCCTGCGAAGTGTGCACTCCACGGGAAGAGAACAGTGGGGTTCTCTGCTCATGCTTGAACTGCTTTCTTGCAGGGGGTGTCTTGTACCGTTTTGAATATTCTGTCAACCCTGCCCTATTTCTGGCAAAAGCCCGGGGAGGAACGTGCACCCTTGCAGTGTCGGACTCAGATGATATTGTGGTCCATCGAGCAAAATACCTGCTTGAGAATGGCTTTGGTTGCTATTGCATATTTAAGAACAATTGTGAAGACTTCGCTATTTATTGCAAAACCGGGCTGCTTGTTATGGATCAAAGAACATTAGGGCAAAGTGGTCAAGCAGCATCTATAATAGGGGGACCTCTTGCAGCCGTTTTAGCAACACCACTGCGTCTTGTCACCACCAACCTTTATGGGATGGTAGCTGTAGGCATTGGAGTATACTGCGCTAGTAGGTACAGTGCTGACATTGGCAAGAGGATGGATGTCGTGAAGGTGTCTGTGGAGGATATGACAACGAGACTTGCAGCTGGCTCGCTCCAGGTGGTGGAGGCCCAAATCTCAGCTCCCTTGCTCCAAGCACCCGAGTCCCAAATCCCAGCTCCCTTGCTCCAGGCGCCCGAGTCCCAAATCCCAGCGCCCTCTACAAGAGGTTCTTCCAAACTTTTCTTCTGGTCAAAAAGTTCTTCCAACCTTGTCACTCGGTAG

Protein Analysis

298

Amino Acids

32.03

Weight (kDa)

8.45

Isoelectric Point (pI)

33.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 13 - 164 1.8e-27 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 417
AccB1I GGYRCC 1 cut(s) 805
AccB7I CCANNNNNTGG 1 cut(s) 736
AccI GTMKAC 1 cut(s) 630
AclWI GGATC 2 cut(s) 57, 507
AcoI YGGCCR 1 cut(s) 139
AcyI GRCGYC 1 cut(s) 806
AfaI GTAC 2 cut(s) 296, 647
AfiI CCNNNNNNNGG 2 cut(s) 331, 736
AgsI TTSAA 2 cut(s) 268, 305
AjiI CACGTC 1 cut(s) 137
AjnI CCWGG 3 cut(s) 43, 729, 801
AjuI GAANNNNNNNTTGG 2 cut(s) 842, 874
AluBI AGCT 5 cut(s) 186, 614, 719, 755, 791
AluI AGCT 5 cut(s) 186, 614, 719, 755, 791
Alw21I GWGCWC 2 cut(s) 227, 359
Alw26I GTCTC 1 cut(s) 703
Alw44I GTGCAC 2 cut(s) 223, 355
AlwI GGATC 2 cut(s) 57, 507
Ama87I CYCGRG 3 cut(s) 342, 773, 809
AoxI GGCC 2 cut(s) 139, 741
ApaLI GTGCAC 2 cut(s) 223, 355
ApeKI GCWGC 5 cut(s) 7, 487, 530, 557, 716
AspLEI GCGC 3 cut(s) 638, 808, 829
AspS9I GGNCC 4 cut(s) 191, 391, 548, 742
AsuC2I CCSGG 3 cut(s) 343, 344, 484
AsuHPI GGTGA 2 cut(s) 121, 580
AvaI CYCGRG 3 cut(s) 342, 773, 809
AvaII GGWCC 3 cut(s) 191, 391, 548
BaeGI GKGCMC 2 cut(s) 227, 359
BalI TGGCCA 1 cut(s) 141
BanI GGYRCC 1 cut(s) 805
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 2 cut(s) 227, 359
BbvI GCAGC 4 cut(s) 474, 542, 569, 728
BccI CCATC 4 cut(s) 96, 402, 601, 664
BceAI ACGGC 1 cut(s) 545
BcgI CGANNNNNNTGC 2 cut(s) 705, 739
BciT130I CCWGG 3 cut(s) 45, 731, 803
BcnI CCSGG 3 cut(s) 343, 344, 484
BcoDI GTCTC 1 cut(s) 703
BfaI CTAG 1 cut(s) 639
BfmI CTRYAG 1 cut(s) 615
BfoI RGCGCY 2 cut(s) 809, 830
BfuAI ACCTGC 1 cut(s) 417
BisI GCNGC 5 cut(s) 8, 488, 531, 558, 717
BlsI GCNGC 5 cut(s) 9, 489, 532, 559, 718
Bme1390I CCNGG 6 cut(s) 45, 343, 344, 484, 731, 803
Bme18I GGWCC 3 cut(s) 191, 391, 548
BmeT110I CYCGRG 3 cut(s) 342, 773, 809
BmgBI CACGTC 1 cut(s) 137
BmgT120I GGNCC 4 cut(s) 191, 391, 548, 742
BmiI GGNNCC 2 cut(s) 549, 807
BmrFI CCNGG 6 cut(s) 45, 343, 344, 484, 731, 803
BmrI ACTGGG 1 cut(s) 165
BmsI GCATC 2 cut(s) 105, 542
BmuI ACTGGG 1 cut(s) 165
BpiI GAAGAC 1 cut(s) 465
BpmI CTGGAG 2 cut(s) 713, 785
Bpu10I CCTNAGC 2 cut(s) 27, 187
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 3 cut(s) 343, 344, 484
BsaHI GRCGYC 1 cut(s) 806
BsaJI CCNNGG 4 cut(s) 44, 230, 342, 343
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 736
Bse1I ACTGG 2 cut(s) 160, 194
BseBI CCWGG 3 cut(s) 45, 731, 803
BseDI CCNNGG 4 cut(s) 44, 230, 342, 343
BseGI GGATG 5 cut(s) 115, 175, 612, 675, 679
BseLI CCNNNNNNNGG 2 cut(s) 331, 736
BseMII CTCAG 3 cut(s) 201, 390, 765
BseNI ACTGG 2 cut(s) 160, 194
BseSI GKGCMC 2 cut(s) 227, 359
BseXI GCAGC 4 cut(s) 474, 542, 569, 728
BseYI CCCAGC 2 cut(s) 787, 823
BshFI GGCC 2 cut(s) 141, 743
BshNI GGYRCC 1 cut(s) 805
BsiHKAI GWGCWC 2 cut(s) 227, 359
BsiHKCI CYCGRG 3 cut(s) 342, 773, 809
BsiSI CCGG 2 cut(s) 343, 483
BslFI GGGAC 4 cut(s) 172, 561, 763, 799
BslI CCNNNNNNNGG 2 cut(s) 331, 736
BsmAI GTCTC 1 cut(s) 703
BsmFI GGGAC 4 cut(s) 172, 561, 763, 799
BsnI GGCC 2 cut(s) 141, 743
BsoBI CYCGRG 3 cut(s) 342, 773, 809
Bsp1286I GDGCHC 2 cut(s) 227, 359
Bsp143I GATC 2 cut(s) 49, 499
BspANI GGCC 2 cut(s) 141, 743
BspCNI CTCAG 3 cut(s) 200, 389, 764
BspLI GGNNCC 2 cut(s) 549, 807
BspMI ACCTGC 1 cut(s) 417
BspPI GGATC 2 cut(s) 57, 507
BspT107I GGYRCC 1 cut(s) 805
BsrI ACTGG 2 cut(s) 160, 194
BssECI CCNNGG 4 cut(s) 44, 230, 342, 343
BssMI GATC 2 cut(s) 49, 499
BssNAI GTATAC 1 cut(s) 631
BssNI GRCGYC 1 cut(s) 806
Bst1107I GTATAC 1 cut(s) 631
Bst2UI CCWGG 3 cut(s) 45, 731, 803
Bst4CI ACNGT 4 cut(s) 163, 245, 299, 650
Bst6I CTCTTC 1 cut(s) 231
BstACI GRCGYC 1 cut(s) 806
BstC8I GCNNGC 2 cut(s) 721, 725
BstDEI CTNAG 4 cut(s) 27, 187, 376, 751
BstDSI CCRYGG 1 cut(s) 230
BstF5I GGATG 5 cut(s) 115, 175, 612, 675, 679
BstH2I RGCGCY 2 cut(s) 809, 830
BstHHI GCGC 3 cut(s) 638, 808, 829
BstKTI GATC 2 cut(s) 52, 502
BstMAI GTCTC 1 cut(s) 703
BstMBI GATC 2 cut(s) 49, 499
BstMWI GCNNNNNNNGC 5 cut(s) 222, 270, 429, 566, 805
BstNI CCWGG 3 cut(s) 45, 731, 803
BstSCI CCNGG 6 cut(s) 43, 341, 342, 482, 729, 801
BstSFI CTRYAG 1 cut(s) 615
BstSLI GKGCMC 2 cut(s) 227, 359
BstV1I GCAGC 4 cut(s) 474, 542, 569, 728
BstV2I GAAGAC 1 cut(s) 465
BstXI CCANNNNNNTGG 1 cut(s) 22
BstZ17I GTATAC 1 cut(s) 631
BsuRI GGCC 2 cut(s) 141, 743
BtgI CCRYGG 1 cut(s) 230
BtrI CACGTC 1 cut(s) 137
BtsCI GGATG 5 cut(s) 115, 175, 612, 675, 679
BtsI GCAGTG 2 cut(s) 372, 575
BtsIMutI CAGTG 5 cut(s) 168, 250, 372, 575, 655
BveI ACCTGC 1 cut(s) 417
Cac8I GCNNGC 2 cut(s) 721, 725
CfoI GCGC 3 cut(s) 638, 808, 829
Cfr13I GGNCC 4 cut(s) 191, 391, 548, 742
Cfr9I CCCGGG 1 cut(s) 342
CseI GACGC 1 cut(s) 569
Csp6I GTAC 2 cut(s) 295, 646
CviAII CATG 2 cut(s) 92, 261
CviQI GTAC 2 cut(s) 295, 646
DdeI CTNAG 4 cut(s) 27, 187, 376, 751
DinI GGCGCC 1 cut(s) 807
DpnI GATC 2 cut(s) 51, 501
DpnII GATC 2 cut(s) 49, 499
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
Eco47I GGWCC 3 cut(s) 191, 391, 548
Eco88I CYCGRG 3 cut(s) 342, 773, 809
EcoO109I RGGNCCY 1 cut(s) 548
EcoRII CCWGG 3 cut(s) 43, 729, 801
EgeI GGCGCC 1 cut(s) 807
EheI GGCGCC 1 cut(s) 807
FaeI CATG 2 cut(s) 95, 264
FalI AAGNNNNNCTT 2 cut(s) 829, 861
FaqI GGGAC 4 cut(s) 172, 561, 763, 799
FatI CATG 2 cut(s) 91, 260
FblI GTMKAC 1 cut(s) 630
Fnu4HI GCNGC 5 cut(s) 8, 488, 531, 558, 717
FokI GGATG 5 cut(s) 122, 182, 619, 682, 686
Fsp4HI GCNGC 5 cut(s) 8, 488, 531, 558, 717
FspBI CTAG 1 cut(s) 639
GlaI GCGC 3 cut(s) 637, 807, 828
GluI GCNGC 5 cut(s) 8, 488, 531, 558, 717
GsaI CCCAGC 2 cut(s) 791, 827
GsuI CTGGAG 2 cut(s) 713, 785
HaeII RGCGCY 2 cut(s) 809, 830
HaeIII GGCC 2 cut(s) 141, 743
HapII CCGG 2 cut(s) 343, 483
HgaI GACGC 1 cut(s) 569
HhaI GCGC 3 cut(s) 638, 808, 829
Hin1I GRCGYC 1 cut(s) 806
Hin1II CATG 2 cut(s) 95, 264
Hin6I GCGC 3 cut(s) 636, 806, 827
HinP1I GCGC 3 cut(s) 636, 806, 827
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HinfI GANTC 3 cut(s) 374, 776, 812
HpaII CCGG 2 cut(s) 343, 483
HphI GGTGA 2 cut(s) 121, 580
Hpy166II GTNNAC 4 cut(s) 225, 316, 357, 631
Hpy188I TCNGA 3 cut(s) 200, 373, 379
Hpy188III TCNNGA 2 cut(s) 189, 679
Hpy8I GTNNAC 4 cut(s) 225, 316, 357, 631
HpyAV CCTTC 1 cut(s) 676
HpyCH4III ACNGT 4 cut(s) 163, 245, 299, 650
HpyCH4IV ACGT 2 cut(s) 136, 353
HpyCH4V TGCA 8 cut(s) 225, 281, 357, 365, 438, 477, 557, 716
HpyF10VI GCNNNNNNNGC 5 cut(s) 222, 270, 429, 566, 805
HpyF3I CTNAG 4 cut(s) 27, 187, 376, 751
HpySE526I ACGT 2 cut(s) 136, 353
Hsp92I GRCGYC 1 cut(s) 806
Hsp92II CATG 2 cut(s) 95, 264
HspAI GCGC 3 cut(s) 636, 806, 827
KasI GGCGCC 1 cut(s) 805
Kzo9I GATC 2 cut(s) 49, 499
LmnI GCTCC 5 cut(s) 732, 760, 768, 796, 804
Lsp1109I GCAGC 4 cut(s) 474, 542, 569, 728
LweI GCATC 2 cut(s) 105, 542
MaeI CTAG 1 cut(s) 639
MaeII ACGT 2 cut(s) 136, 353
MaeIII GTNAC 2 cut(s) 586, 886
MalI GATC 2 cut(s) 51, 501
MboI GATC 2 cut(s) 49, 499
MboII GAAGA 5 cut(s) 248, 470, 837, 850, 867
MfeI CAATTG 1 cut(s) 451
MhlI GDGCHC 2 cut(s) 227, 359
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 1 cut(s) 451
MluNI TGGCCA 1 cut(s) 141
Mly113I GGCGCC 1 cut(s) 806
MlyI GAGTC 3 cut(s) 368, 785, 821
MmeI TCCRAC 4 cut(s) 39, 147, 223, 351
MnlI CCTC 9 cut(s) 60, 213, 341, 561, 660, 688, 733, 833, 841
Mox20I TGGCCA 1 cut(s) 141
MscI TGGCCA 1 cut(s) 141
MseI TTAA 2 cut(s) 39, 444
Msp20I TGGCCA 1 cut(s) 141
MspA1I CMGCKG 1 cut(s) 719
MspI CCGG 2 cut(s) 343, 483
MspR9I CCNGG 6 cut(s) 45, 343, 344, 484, 731, 803
MunI CAATTG 1 cut(s) 451
MvaI CCWGG 3 cut(s) 45, 731, 803
MwoI GCNNNNNNNGC 5 cut(s) 222, 270, 429, 566, 805
NarI GGCGCC 1 cut(s) 806
NciI CCSGG 3 cut(s) 343, 344, 484
NdeII GATC 2 cut(s) 49, 499
NlaIII CATG 2 cut(s) 95, 264
NlaIV GGNNCC 2 cut(s) 549, 807
NmuCI GTSAC 2 cut(s) 586, 886
PflFI GACNNNGTC 1 cut(s) 116
PflMI CCANNNNNTGG 1 cut(s) 736
PkrI GCNGC 5 cut(s) 9, 489, 532, 559, 718
PleI GAGTC 3 cut(s) 368, 784, 820
PluTI GGCGCC 1 cut(s) 809
PpsI GAGTC 3 cut(s) 368, 784, 820
PpuMI RGGWCCY 1 cut(s) 548
Psp5II RGGWCCY 1 cut(s) 548
Psp6I CCWGG 3 cut(s) 43, 729, 801
PspFI CCCAGC 2 cut(s) 787, 823
PspGI CCWGG 3 cut(s) 43, 729, 801
PspN4I GGNNCC 2 cut(s) 549, 807
PspPI GGNCC 4 cut(s) 191, 391, 548, 742
PspPPI RGGWCCY 1 cut(s) 548
PsyI GACNNNGTC 1 cut(s) 116
PvuII CAGCTG 1 cut(s) 719
RsaI GTAC 2 cut(s) 296, 647
RsaNI GTAC 2 cut(s) 295, 646
SaqAI TTAA 2 cut(s) 39, 444
SatI GCNGC 5 cut(s) 8, 488, 531, 558, 717
Sau3AI GATC 2 cut(s) 49, 499
Sau96I GGNCC 4 cut(s) 191, 391, 548, 742
SchI GAGTC 3 cut(s) 368, 785, 821
ScrFI CCNGG 6 cut(s) 45, 343, 344, 484, 731, 803
SduI GDGCHC 2 cut(s) 227, 359
SfaNI GCATC 2 cut(s) 105, 542
SfcI CTRYAG 1 cut(s) 615
SfoI GGCGCC 1 cut(s) 807
SinI GGWCC 3 cut(s) 191, 391, 548
SmaI CCCGGG 1 cut(s) 344
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
Sse9I AATT 1 cut(s) 451
SspDI GGCGCC 1 cut(s) 805
SspI AATATT 1 cut(s) 308
SspMI CTAG 1 cut(s) 639
StyD4I CCNGG 6 cut(s) 43, 341, 342, 482, 729, 801
TaaI ACNGT 4 cut(s) 163, 245, 299, 650
TaiI ACGT 2 cut(s) 139, 356
TaqI TCGA 1 cut(s) 397
TasI AATT 1 cut(s) 451
Tru1I TTAA 2 cut(s) 39, 444
Tru9I TTAA 2 cut(s) 39, 444
TscAI CASTG 5 cut(s) 168, 250, 372, 582, 655
TseFI GTSAC 2 cut(s) 586, 886
TseI GCWGC 5 cut(s) 7, 487, 530, 557, 716
Tsp45I GTSAC 2 cut(s) 586, 886
TspDTI ATGAA 1 cut(s) 113
TspMI CCCGGG 1 cut(s) 342
TspRI CASTG 5 cut(s) 168, 250, 372, 582, 655
Tth111I GACNNNGTC 1 cut(s) 116
Van91I CCANNNNNTGG 1 cut(s) 736
VneI GTGCAC 2 cut(s) 223, 355
VpaK11BI GGWCC 3 cut(s) 191, 391, 548
XmaI CCCGGG 1 cut(s) 342
XmiI GTMKAC 1 cut(s) 630
XspI CTAG 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.