RchiOBHm_Chr4g0406031

Belongs to the SNF7 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0007790
Physical Location & Seq
Reverse (-)
27092814 .. 27096545
3732 bp
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UTR
Exon/CDS
Intron
PRQ37747

Sequence Viewer

Length: 663 bp
ATGGGGAATTTGTTCGTGAAGAAACCCAAGATCACCGATGTCGATCGAGCTATTCTCTCTCTCAAGACCCAGAGACGCAAGCTCGGTCAATACCAACAACAGCTTGAAGCTGTAATTGAAGCAGAAAAGCAGGCTGCAAGGGACTTGATTCGTGATAAGAAAAAGGAAAGAGCCTTACTAGCATTGAAGAAGAAAAAGGCACAAGAAGAATTATTGAAGCAAGTAGATACCTGGGTTATTAATGTTGAGCAACAATTGCAAGATATTGAACTATCGAGCAAGCAGAAAGCTGTATTTGAGAGTTTGAAGGCCGGTAATAATGCAATTAAAGCTATACAAAGTGAGATCAACCTTGAGGATGTTCAAAAGCTAATGGATGATACTGAGGAAGCTAAAGTTTACCAAGATGAAATTAGTGCAATTTTGGGGGAGAAATTATCAACCGAAGATGAAGAGGAAATTTTAGCAGAATTTGACAACTTGGAAGCTCAGATCACTGTTCAAGACCTTCCTGAAGCTCCTGCTTCGGTGCCAGGTCATCAAGATGAGAAGTTGGACCTTCCCGATGTACCAACCAAAGTACCAGTTGCACCTGATGTGGTCACAGATGATGCAAAAGATTCAGCTAAGAGTAAAGTTATGGAGGAACCGGTGTTGGCTTGA

Protein Analysis

220

Amino Acids

24.67

Weight (kDa)

4.73

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Snf7 PF03357 17 - 193 1.2e-41 Snf7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 529
AcsI RAATTY 3 cut(s) 7, 459, 470
AcuI CTGAAG 1 cut(s) 534
AfaI GTAC 2 cut(s) 570, 582
AgeI ACCGGT 1 cut(s) 649
AgsI TTSAA 8 cut(s) 107, 119, 187, 217, 269, 307, 365, 503
AjnI CCWGG 2 cut(s) 230, 532
Alw26I GTCTC 1 cut(s) 67
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 3 cut(s) 7, 459, 470
AseI ATTAAT 1 cut(s) 240
AsiGI ACCGGT 1 cut(s) 649
Asp700I GAANNNNTTC 1 cut(s) 11
AspS9I GGNCC 1 cut(s) 556
AsuHPI GGTGA 1 cut(s) 25
AvaII GGWCC 1 cut(s) 556
BaeI ACNNNNGTAYC 4 cut(s) 219, 252, 564, 597
BanI GGYRCC 1 cut(s) 529
BbvI GCAGC 1 cut(s) 121
BciT130I CCWGG 2 cut(s) 232, 534
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 1 cut(s) 179
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
Bme1390I CCNGG 2 cut(s) 232, 534
Bme18I GGWCC 1 cut(s) 556
BmgT120I GGNCC 1 cut(s) 556
BmiI GGNNCC 2 cut(s) 531, 648
BmrFI CCNGG 2 cut(s) 232, 534
BmsI GCATC 1 cut(s) 601
BplI GAGNNNNNCTC 2 cut(s) 39, 71
BpuEI CTTGAG 2 cut(s) 47, 374
BsaBI GATNNNNATC 1 cut(s) 42
BsaJI CCNNGG 1 cut(s) 231
BsaWI WCCGGW 1 cut(s) 649
Bse118I RCCGGY 2 cut(s) 311, 649
Bse1I ACTGG 1 cut(s) 584
Bse8I GATNNNNATC 1 cut(s) 42
BseBI CCWGG 2 cut(s) 232, 534
BseDI CCNNGG 1 cut(s) 231
BseGI GGATG 2 cut(s) 364, 382
BseJI GATNNNNATC 1 cut(s) 42
BseMII CTCAG 2 cut(s) 375, 503
BseNI ACTGG 1 cut(s) 584
BseXI GCAGC 1 cut(s) 121
Bsh1285I CGRYCG 1 cut(s) 46
BshFI GGCC 1 cut(s) 311
BshNI GGYRCC 1 cut(s) 529
BshTI ACCGGT 1 cut(s) 649
BsiEI CGRYCG 1 cut(s) 46
BsiSI CCGG 2 cut(s) 312, 650
BslFI GGGAC 1 cut(s) 155
BsmAI GTCTC 1 cut(s) 67
BsmBI CGTCTC 1 cut(s) 67
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 1 cut(s) 311
Bsp143I GATC 4 cut(s) 30, 43, 345, 492
BspANI GGCC 1 cut(s) 311
BspCNI CTCAG 2 cut(s) 376, 502
BspLI GGNNCC 2 cut(s) 531, 648
BspT107I GGYRCC 1 cut(s) 529
BsrFI RCCGGY 2 cut(s) 311, 649
BsrI ACTGG 1 cut(s) 584
BssAI RCCGGY 2 cut(s) 311, 649
BssECI CCNNGG 1 cut(s) 231
BssMI GATC 4 cut(s) 30, 43, 345, 492
Bst2UI CCWGG 2 cut(s) 232, 534
Bst4CI ACNGT 1 cut(s) 499
Bst6I CTCTTC 1 cut(s) 447
BstAPI GCANNNNNTGC 1 cut(s) 256
BstC8I GCNNGC 3 cut(s) 80, 132, 281
BstDEI CTNAG 3 cut(s) 384, 489, 627
BstF5I GGATG 2 cut(s) 364, 382
BstKTI GATC 4 cut(s) 33, 46, 348, 495
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 4 cut(s) 30, 43, 345, 492
BstMCI CGRYCG 1 cut(s) 46
BstMWI GCNNNNNNNGC 3 cut(s) 179, 256, 329
BstNI CCWGG 2 cut(s) 232, 534
BstSCI CCNGG 2 cut(s) 230, 532
BstV1I GCAGC 1 cut(s) 121
BsuRI GGCC 1 cut(s) 311
BtsCI GGATG 2 cut(s) 364, 382
BtsIMutI CAGTG 1 cut(s) 495
Cac8I GCNNGC 3 cut(s) 80, 132, 281
Cfr10I RCCGGY 2 cut(s) 311, 649
Cfr13I GGNCC 1 cut(s) 556
CseI GACGC 1 cut(s) 84
Csp6I GTAC 2 cut(s) 569, 581
CspAI ACCGGT 1 cut(s) 649
CviQI GTAC 2 cut(s) 569, 581
DdeI CTNAG 3 cut(s) 384, 489, 627
DpnI GATC 4 cut(s) 32, 45, 347, 494
DpnII GATC 4 cut(s) 30, 43, 345, 492
Eam1104I CTCTTC 1 cut(s) 447
EarI CTCTTC 1 cut(s) 447
Eco47I GGWCC 1 cut(s) 556
Eco57I CTGAAG 1 cut(s) 534
EcoRII CCWGG 2 cut(s) 230, 532
Esp3I CGTCTC 1 cut(s) 67
FaiI YATR 2 cut(s) 335, 641
FaqI GGGAC 1 cut(s) 155
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 2 cut(s) 371, 389
Fsp4HI GCNGC 1 cut(s) 135
FspBI CTAG 1 cut(s) 179
GluI GCNGC 1 cut(s) 135
HaeIII GGCC 1 cut(s) 311
HapII CCGG 2 cut(s) 312, 650
HgaI GACGC 1 cut(s) 84
HinfI GANTC 2 cut(s) 148, 620
HpaII CCGG 2 cut(s) 312, 650
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 1 cut(s) 492
Hpy188III TCNNGA 7 cut(s) 16, 64, 152, 503, 512, 542, 563
Hpy8I GTNNAC 1 cut(s) 400
HpyAV CCTTC 3 cut(s) 301, 518, 569
HpyCH4III ACNGT 1 cut(s) 499
HpyCH4V TGCA 6 cut(s) 137, 259, 323, 419, 590, 614
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 256, 329
HpyF3I CTNAG 3 cut(s) 384, 489, 627
Kzo9I GATC 4 cut(s) 30, 43, 345, 492
LmnI GCTCC 1 cut(s) 523
Lsp1109I GCAGC 1 cut(s) 121
LweI GCATC 1 cut(s) 601
MaeI CTAG 1 cut(s) 179
MaeIII GTNAC 1 cut(s) 601
MalI GATC 4 cut(s) 32, 45, 347, 494
MboI GATC 4 cut(s) 30, 43, 345, 492
MboII GAAGA 6 cut(s) 31, 199, 202, 218, 458, 464
MfeI CAATTG 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 534
MnlI CCTC 4 cut(s) 349, 379, 448, 637
MroXI GAANNNNTTC 1 cut(s) 11
MseI TTAA 2 cut(s) 240, 327
MslI CAYNNNNRTG 1 cut(s) 543
MspI CCGG 2 cut(s) 312, 650
MspR9I CCNGG 2 cut(s) 232, 534
MunI CAATTG 1 cut(s) 254
MvaI CCWGG 2 cut(s) 232, 534
MwoI GCNNNNNNNGC 3 cut(s) 179, 256, 329
NdeII GATC 4 cut(s) 30, 43, 345, 492
NlaIV GGNNCC 2 cut(s) 531, 648
NmuCI GTSAC 1 cut(s) 601
PdmI GAANNNNTTC 1 cut(s) 11
PfeI GAWTC 2 cut(s) 148, 620
PinAI ACCGGT 1 cut(s) 649
PkrI GCNGC 1 cut(s) 136
Ple19I CGATCG 1 cut(s) 46
PshBI ATTAAT 1 cut(s) 240
Psp6I CCWGG 2 cut(s) 230, 532
PspGI CCWGG 2 cut(s) 230, 532
PspN4I GGNNCC 2 cut(s) 531, 648
PspPI GGNCC 1 cut(s) 556
PvuI CGATCG 1 cut(s) 46
RsaI GTAC 2 cut(s) 570, 582
RsaNI GTAC 2 cut(s) 569, 581
RseI CAYNNNNRTG 1 cut(s) 543
SaqAI TTAA 2 cut(s) 240, 327
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 4 cut(s) 30, 43, 345, 492
Sau96I GGNCC 1 cut(s) 556
ScrFI CCNGG 2 cut(s) 232, 534
SfaNI GCATC 1 cut(s) 601
SinI GGWCC 1 cut(s) 556
SmiMI CAYNNNNRTG 1 cut(s) 543
SmlI CTYRAG 2 cut(s) 62, 353
SmoI CTYRAG 2 cut(s) 62, 353
SspMI CTAG 1 cut(s) 179
StyD4I CCNGG 2 cut(s) 230, 532
TaaI ACNGT 1 cut(s) 499
TaqI TCGA 3 cut(s) 42, 46, 275
TaqII GACCGA 1 cut(s) 74
TfiI GAWTC 2 cut(s) 148, 620
Tru1I TTAA 2 cut(s) 240, 327
Tru9I TTAA 2 cut(s) 240, 327
TscAI CASTG 1 cut(s) 502
TseFI GTSAC 1 cut(s) 601
TseI GCWGC 1 cut(s) 134
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 2 cut(s) 423, 465
TspRI CASTG 1 cut(s) 502
VpaK11BI GGWCC 1 cut(s) 556
VspI ATTAAT 1 cut(s) 240
XapI RAATTY 3 cut(s) 7, 459, 470
XmnI GAANNNNTTC 1 cut(s) 11
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.