RchiOBHm_Chr5g0034391

Leucine-rich repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003583
Physical Location & Seq
Forward (+)
28262564 .. 28271619
9056 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31340

Sequence Viewer

Length: 1752 bp
ATGGATCGGGTCATCAAAGCCGCCAGAGCCTCCGGTTCTCTCAACCTTTCAAGTCGCTCTCTCAGGTTCGTGCCCGATGAGGTATACAAAAGCGTGAATGCGGTTGGTGAAGACGAGAAGTGGTGGGAGGCTGTGGAGCTACACAAGCTGATTCTAGCTCATAATGAAATTGAATTGCTCAAAGATGAGCTGAGGAATTTGCCTCTACTGACTGTGTTGAATGTCAGCCACAATAAACTTTCCGAGCTTCCGGCGGCAATTGGAGAGCTTACCATGCTGAAATCGTTAGACGTGTCATTCAACTCGATTCTAGAGCTGCCTGAGGAAATTGGATCAGCAACTGCTCTTGTCAAATTTGATTGTTCAAATAATCAGCTTAAGGACCTCCCAAACTCACTTGGGAGATGCTCAGATCTATCAGATTTCAAGGCATCAAACAATGTTATCATCAGCTTGCCAGAAGATCTAGCAAATTGCTCAAAATTGATGAAATTGGATGTGGAGGGAAACAAGTTAACAAACCTATCTGAGAAGTTGATTGGATCGTGGACTTTGCTTACAGAACTCAATGCATCAAAAAATCTGCTGAGTGGAATACCAGAGAATATTGGAAGCCTTTCTCGTCTTATTCGTCTTGACCTTCACCAGAACAAAATTTCAGCGATCCCAGTTTCAATAAGGGGTTGTTGTTCCCTTGCGGAGGTCCACATGGGGAATAATGCACTCTCTACTTTACCATCAGAGATGGGGGAACTTTCTCGCCTAGGAACTCTAGATCTGCAATCAAACCAGTTGAAGGAGTATCCTGTGGAGGCATGTAAATTGCGTCTTTCATTATTGAATCTTTCAAACAATTCATTGTCTGGATTGCCCCCTGAATTGGGCAAGATGACTACCTTGCGAAGACTTTTACTTACCGGAAACCCAATACGAACTCTACGAAGTACATTGTTGTCTGGACCAACACCTACTTTATTGAAGTATCTGCGAAGTAGGCTTTCTCAGAATGAAGATCCTGAAGCTTCAACTACAACAAAAGAGGATGTGATTGCCATGGCAGCTCGATTGTCTATTGCTTCAAAGGAACTTTCTATGGAAGGGCTGGGTTTGAGTTCTGTCCCATCAGAAGTATGGGAATCGAGTGAGGTCATAAAAGTCGATCTAACTAGGAATTCCATTGAAGAGCTCCCAATTGAACTTTCTTCATGCACCCACCTCCAGACACTAATCTTGTCCAGAAACAAGATTAAAGAGTGGCCAGGTGCAATTTTAAGCTCACTTCGTAATCTTTTGTGTTTGAAGCTGGACAATAATCCACTAAGACAGATACCATCAGATGGATTCCAAGCAACCCCCATGCTTCAGATTCTGGATCTAAGTGGTAATGCAGCTTCGTTACCAGAACACCCTGCATTTTCTAGCTTACCGCATCTGCTAGAGCTTTACCTGAGACGAATGCAGCTACGCGAAGTCCCATCAGATATCTTGAGTTTGCAGCAACTGCGGATCCTAGACCTCAGCCAAAATTCCCTGCAGTCAGTTCCAGTGGAGTTAAAATTTTTTACTTCACTCACTGAGCTAGGCCTATCTGACAACAACATATCAACACTTCCTCCAGAATTGGGTATGCTTGAACCCAACTTACAGGCCTTAAGACTTGACGGAAATCCATTAAGAAGCATCCGGCGGACGGTTTTAGATAGAGGAACCAAAGCTGTTCTGCAATATCTCAAGGACAAAATCGTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_1 LRR_5 LRR_8

Protein Analysis

583

Amino Acids

64.33

Weight (kDa)

5.72

Isoelectric Point (pI)

51.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 45 - 116 9.4e-07 Leucine-rich repeat region
LRR_8 PF13855 46 - 103 2.9e-07 Leucine rich repeat
LRR_8 PF13855 161 - 219 8.2e-06 Leucine rich repeat
LRR_14 PF23598 238 - 305 1.7e-06 Leucine-rich repeat region
LRR_14 PF23598 387 - 459 3.5e-06 Leucine-rich repeat region
LRR_8 PF13855 405 - 462 1.5e-09 Leucine rich repeat
LRR_14 PF23598 452 - 550 9.9e-06 Leucine-rich repeat region
LRR_8 PF13855 476 - 534 2e-07 Leucine rich repeat
LRR_8 PF13855 500 - 558 1.8e-08 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1337
AccI GTMKAC 1 cut(s) 84
AccII CGCG 1 cut(s) 1467
AciI CCGC 7 cut(s) 21, 101, 254, 698, 1427, 1504, 1687
AclWI GGATC 8 cut(s) 12, 340, 550, 658, 1007, 1380, 1501, 1514
AcoI YGGCCR 1 cut(s) 1256
AcsI RAATTY 6 cut(s) 196, 353, 654, 1171, 1525, 1556
AcuI CTGAAG 2 cut(s) 1038, 1346
AfaI GTAC 1 cut(s) 946
AfiI CCNNNNNNNGG 7 cut(s) 700, 796, 880, 881, 1337, 1622, 1690
AflII CTTAAG 2 cut(s) 377, 1651
AflIII ACRYGT 1 cut(s) 291
AjiI CACGTC 1 cut(s) 292
AjnI CCWGG 1 cut(s) 1258
AjuI GAANNNNNNNTTGG 2 cut(s) 1183, 1215
Alw21I GWGCWC 1 cut(s) 1188
Alw26I GTCTC 1 cut(s) 1444
AlwI GGATC 8 cut(s) 12, 340, 550, 658, 1007, 1380, 1501, 1514
AlwNI CAGNNNCTG 3 cut(s) 341, 1369, 1501
AoxI GGCC 3 cut(s) 1256, 1582, 1647
ApeKI GCWGC 5 cut(s) 316, 1058, 1388, 1459, 1495
ApoI RAATTY 6 cut(s) 196, 353, 654, 1171, 1525, 1556
Asp700I GAANNNNTTC 1 cut(s) 616
AspA2I CCTAGG 1 cut(s) 763
AspS9I GGNCC 3 cut(s) 382, 703, 959
AsuHPI GGTGA 2 cut(s) 119, 635
AvaII GGWCC 3 cut(s) 382, 703, 959
AvrII CCTAGG 1 cut(s) 763
AxyI CCTNAGG 1 cut(s) 321
BaeGI GKGCMC 1 cut(s) 75
BalI TGGCCA 1 cut(s) 1258
BamHI GGATCC 1 cut(s) 1506
BanII GRGCYC 1 cut(s) 1188
BarI GAAGNNNNNNTAC 2 cut(s) 1375, 1407
BbsI GAAGAC 2 cut(s) 117, 910
Bbv12I GWGCWC 1 cut(s) 1188
BbvCI CCTCAGC 2 cut(s) 191, 1517
BbvI GCAGC 5 cut(s) 303, 1070, 1400, 1471, 1507
BccI CCATC 6 cut(s) 739, 745, 1129, 1331, 1339, 1483
BciT130I CCWGG 1 cut(s) 1260
BciVI GTATCC 1 cut(s) 813
BcoDI GTCTC 1 cut(s) 1444
BfmI CTRYAG 1 cut(s) 1532
BfrI CTTAAG 2 cut(s) 377, 1651
BfuI GTATCC 1 cut(s) 813
BglII AGATCT 3 cut(s) 412, 463, 775
BisI GCNGC 7 cut(s) 21, 255, 317, 1059, 1389, 1460, 1496
BlnI CCTAGG 1 cut(s) 763
BlsI GCNGC 7 cut(s) 22, 256, 318, 1060, 1390, 1461, 1497
Bme1390I CCNGG 1 cut(s) 1260
Bme18I GGWCC 3 cut(s) 382, 703, 959
BmgBI CACGTC 1 cut(s) 292
BmgT120I GGNCC 3 cut(s) 382, 703, 959
BmiI GGNNCC 2 cut(s) 1508, 1708
BmrFI CCNGG 1 cut(s) 1260
BmrI ACTGGG 1 cut(s) 662
BmsI GCATC 5 cut(s) 395, 440, 581, 1438, 1689
BmuI ACTGGG 1 cut(s) 662
BpiI GAAGAC 2 cut(s) 117, 910
BpmI CTGGAG 2 cut(s) 1202, 1599
Bpu10I CCTNAGC 2 cut(s) 191, 1517
BpuEI CTTGAG 2 cut(s) 1507, 1715
BsaJI CCNNGG 2 cut(s) 763, 1053
BsaWI WCCGGW 2 cut(s) 32, 917
BsaXI ACNNNNNCTCC 2 cut(s) 1597, 1627
Bsc4I CCNNNNNNNGG 7 cut(s) 700, 796, 880, 881, 1337, 1622, 1690
Bse1I ACTGG 3 cut(s) 668, 790, 1544
Bse21I CCTNAGG 1 cut(s) 321
BseBI CCWGG 1 cut(s) 1260
BseDI CCNNGG 2 cut(s) 763, 1053
BseGI GGATG 3 cut(s) 502, 1048, 1680
BseLI CCNNNNNNNGG 7 cut(s) 700, 796, 880, 881, 1337, 1622, 1690
BseNI ACTGG 3 cut(s) 668, 790, 1544
BseSI GKGCMC 1 cut(s) 75
BseXI GCAGC 5 cut(s) 303, 1070, 1400, 1471, 1507
BseYI CCCAGC 1 cut(s) 1102
Bsh1236I CGCG 1 cut(s) 1467
BshFI GGCC 3 cut(s) 1258, 1584, 1649
BsiHKAI GWGCWC 1 cut(s) 1188
BsiSI CCGG 4 cut(s) 33, 251, 918, 1684
BslFI GGGAC 2 cut(s) 1103, 1457
BslI CCNNNNNNNGG 7 cut(s) 700, 796, 880, 881, 1337, 1622, 1690
BsmAI GTCTC 1 cut(s) 1444
BsmBI CGTCTC 1 cut(s) 1444
BsmFI GGGAC 2 cut(s) 1103, 1457
BsmI GAATGC 2 cut(s) 103, 1461
BsnI GGCC 3 cut(s) 1258, 1584, 1649
Bsp1286I GDGCHC 2 cut(s) 75, 1188
Bsp19I CCATGG 1 cut(s) 1053
BspACI CCGC 7 cut(s) 21, 101, 254, 698, 1427, 1504, 1687
BspANI GGCC 3 cut(s) 1258, 1584, 1649
BspFNI CGCG 1 cut(s) 1467
BspLI GGNNCC 2 cut(s) 1508, 1708
BspMAI CTGCAG 1 cut(s) 1536
BspPI GGATC 8 cut(s) 12, 340, 550, 658, 1007, 1380, 1501, 1514
BspQI GCTCTTC 1 cut(s) 1176
BspTI CTTAAG 2 cut(s) 377, 1651
BsrI ACTGG 3 cut(s) 668, 790, 1544
BssECI CCNNGG 2 cut(s) 763, 1053
BssNAI GTATAC 1 cut(s) 85
BssT1I CCWWGG 2 cut(s) 763, 1053
Bst1107I GTATAC 1 cut(s) 85
Bst2UI CCWGG 1 cut(s) 1260
Bst4CI ACNGT 2 cut(s) 214, 1693
Bst6I CTCTTC 1 cut(s) 1176
BstAFI CTTAAG 2 cut(s) 377, 1651
BstAPI GCANNNNNTGC 1 cut(s) 1501
BstC8I GCNNGC 1 cut(s) 455
BstDSI CCRYGG 1 cut(s) 1053
BstENI CCTNNNNNAGG 1 cut(s) 698
BstF5I GGATG 3 cut(s) 502, 1048, 1680
BstFNI CGCG 1 cut(s) 1467
BstMAI GTCTC 1 cut(s) 1444
BstMWI GCNNNNNNNGC 6 cut(s) 26, 145, 274, 994, 1058, 1501
BstNI CCWGG 1 cut(s) 1260
BstNSI RCATGY 1 cut(s) 819
BstSCI CCNGG 1 cut(s) 1258
BstSFI CTRYAG 1 cut(s) 1532
BstSLI GKGCMC 1 cut(s) 75
BstUI CGCG 1 cut(s) 1467
BstV1I GCAGC 5 cut(s) 303, 1070, 1400, 1471, 1507
BstV2I GAAGAC 2 cut(s) 117, 910
BstX2I RGATCY 6 cut(s) 412, 463, 775, 1012, 1372, 1506
BstYI RGATCY 6 cut(s) 412, 463, 775, 1012, 1372, 1506
BstZ17I GTATAC 1 cut(s) 85
Bsu36I CCTNAGG 1 cut(s) 321
BsuI GTATCC 1 cut(s) 813
BsuRI GGCC 3 cut(s) 1258, 1584, 1649
BtgI CCRYGG 1 cut(s) 1053
BtrI CACGTC 1 cut(s) 292
BtsCI GGATG 3 cut(s) 502, 1048, 1680
BtsIMutI CAGTG 2 cut(s) 1551, 1572
Cac8I GCNNGC 1 cut(s) 455
CaiI CAGNNNCTG 3 cut(s) 341, 1369, 1501
Cfr13I GGNCC 3 cut(s) 382, 703, 959
CseI GACGC 1 cut(s) 815
Csp6I GTAC 1 cut(s) 945
CviAII CATG 6 cut(s) 274, 709, 816, 1054, 1206, 1357
CviQI GTAC 1 cut(s) 945
EaeI YGGCCR 1 cut(s) 1256
Eam1104I CTCTTC 1 cut(s) 1176
EarI CTCTTC 1 cut(s) 1176
EciI GGCGGA 1 cut(s) 1702
Ecl136II GAGCTC 1 cut(s) 1186
Eco130I CCWWGG 2 cut(s) 763, 1053
Eco147I AGGCCT 2 cut(s) 1584, 1649
Eco24I GRGCYC 1 cut(s) 1188
Eco32I GATATC 1 cut(s) 1483
Eco47I GGWCC 3 cut(s) 382, 703, 959
Eco53kI GAGCTC 1 cut(s) 1186
Eco57I CTGAAG 2 cut(s) 1038, 1346
Eco81I CCTNAGG 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 1186
EcoNI CCTNNNNNAGG 1 cut(s) 698
EcoO109I RGGNCCY 1 cut(s) 382
EcoRI GAATTC 1 cut(s) 1171
EcoRII CCWGG 1 cut(s) 1258
EcoRV GATATC 1 cut(s) 1483
EcoT14I CCWWGG 2 cut(s) 763, 1053
EcoT22I ATGCAT 1 cut(s) 574
EcoT38I GRGCYC 1 cut(s) 1188
ErhI CCWWGG 2 cut(s) 763, 1053
Esp3I CGTCTC 1 cut(s) 1444
FaeI CATG 6 cut(s) 277, 712, 819, 1057, 1209, 1360
FaqI GGGAC 2 cut(s) 1103, 1457
FatI CATG 6 cut(s) 273, 708, 815, 1053, 1205, 1356
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 7 cut(s) 21, 255, 317, 1059, 1389, 1460, 1496
FokI GGATG 3 cut(s) 509, 1055, 1667
FriOI GRGCYC 1 cut(s) 1188
Fsp4HI GCNGC 7 cut(s) 21, 255, 317, 1059, 1389, 1460, 1496
GluI GCNGC 7 cut(s) 21, 255, 317, 1059, 1389, 1460, 1496
GsaI CCCAGC 1 cut(s) 1106
GsuI CTGGAG 2 cut(s) 1202, 1599
HaeIII GGCC 3 cut(s) 1258, 1584, 1649
HapII CCGG 4 cut(s) 33, 251, 918, 1684
HgaI GACGC 1 cut(s) 815
Hin1II CATG 6 cut(s) 277, 712, 819, 1057, 1209, 1360
HincII GTYRAC 1 cut(s) 516
HindII GTYRAC 1 cut(s) 516
HindIII AAGCTT 1 cut(s) 1020
HinfI GANTC 6 cut(s) 151, 307, 841, 1136, 1341, 1366
HpaI GTTAAC 1 cut(s) 516
HpaII CCGG 4 cut(s) 33, 251, 918, 1684
HphI GGTGA 2 cut(s) 119, 635
Hpy166II GTNNAC 4 cut(s) 85, 516, 549, 706
Hpy8I GTNNAC 4 cut(s) 85, 516, 549, 706
HpyAV CCTTC 3 cut(s) 650, 790, 1091
HpyCH4III ACNGT 2 cut(s) 214, 1693
HpyCH4IV ACGT 1 cut(s) 291
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 145, 274, 994, 1058, 1501
HpySE526I ACGT 1 cut(s) 291
Hsp92II CATG 6 cut(s) 277, 712, 819, 1057, 1209, 1360
KspAI GTTAAC 1 cut(s) 516
LguI GCTCTTC 1 cut(s) 1176
LmnI GCTCC 2 cut(s) 136, 1191
Lsp1109I GCAGC 5 cut(s) 303, 1070, 1400, 1471, 1507
LweI GCATC 5 cut(s) 395, 440, 581, 1438, 1689
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 1 cut(s) 1395
MboII GAAGA 6 cut(s) 122, 473, 915, 1022, 1193, 1194
MfeI CAATTG 2 cut(s) 258, 1191
MflI RGATCY 6 cut(s) 412, 463, 775, 1012, 1372, 1506
MhlI GDGCHC 2 cut(s) 75, 1188
MlsI TGGCCA 1 cut(s) 1258
MluNI TGGCCA 1 cut(s) 1258
Mox20I TGGCCA 1 cut(s) 1258
Mph1103I ATGCAT 1 cut(s) 574
MroXI GAANNNNTTC 1 cut(s) 616
MscI TGGCCA 1 cut(s) 1258
MseI TTAA 7 cut(s) 378, 515, 1248, 1271, 1553, 1652, 1673
Msp20I TGGCCA 1 cut(s) 1258
MspCI CTTAAG 2 cut(s) 377, 1651
MspI CCGG 4 cut(s) 33, 251, 918, 1684
MspR9I CCNGG 1 cut(s) 1260
MunI CAATTG 2 cut(s) 258, 1191
Mva1269I GAATGC 2 cut(s) 103, 1461
MvaI CCWGG 1 cut(s) 1260
MvnI CGCG 1 cut(s) 1467
MwoI GCNNNNNNNGC 6 cut(s) 26, 145, 274, 994, 1058, 1501
NcoI CCATGG 1 cut(s) 1053
NlaIII CATG 6 cut(s) 277, 712, 819, 1057, 1209, 1360
NlaIV GGNNCC 2 cut(s) 1508, 1708
NsiI ATGCAT 1 cut(s) 574
NspI RCATGY 1 cut(s) 819
PceI AGGCCT 2 cut(s) 1584, 1649
PciSI GCTCTTC 1 cut(s) 1176
PcsI WCGNNNNNNNCGW 2 cut(s) 628, 937
PctI GAATGC 2 cut(s) 103, 1461
PdmI GAANNNNTTC 1 cut(s) 616
PfeI GAWTC 6 cut(s) 151, 307, 841, 1136, 1341, 1366
PflMI CCANNNNNTGG 1 cut(s) 1337
PkrI GCNGC 7 cut(s) 22, 256, 318, 1060, 1390, 1461, 1497
PpuMI RGGWCCY 1 cut(s) 382
Psp124BI GAGCTC 1 cut(s) 1188
Psp5II RGGWCCY 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 1258
PspFI CCCAGC 1 cut(s) 1102
PspGI CCWGG 1 cut(s) 1258
PspN4I GGNNCC 2 cut(s) 1508, 1708
PspPI GGNCC 3 cut(s) 382, 703, 959
PspPPI RGGWCCY 1 cut(s) 382
PsrI GAACNNNNNNTAC 2 cut(s) 1626, 1658
PstI CTGCAG 1 cut(s) 1536
PstNI CAGNNNCTG 3 cut(s) 341, 1369, 1501
PsuI RGATCY 6 cut(s) 412, 463, 775, 1012, 1372, 1506
RsaI GTAC 1 cut(s) 946
RsaNI GTAC 1 cut(s) 945
SacI GAGCTC 1 cut(s) 1188
SapI GCTCTTC 1 cut(s) 1176
SaqAI TTAA 7 cut(s) 378, 515, 1248, 1271, 1553, 1652, 1673
SatI GCNGC 7 cut(s) 21, 255, 317, 1059, 1389, 1460, 1496
Sau96I GGNCC 3 cut(s) 382, 703, 959
ScrFI CCNGG 1 cut(s) 1260
SduI GDGCHC 2 cut(s) 75, 1188
SfaNI GCATC 5 cut(s) 395, 440, 581, 1438, 1689
SfcI CTRYAG 1 cut(s) 1532
SinI GGWCC 3 cut(s) 382, 703, 959
SmlI CTYRAG 4 cut(s) 377, 1486, 1651, 1730
SmoI CTYRAG 4 cut(s) 377, 1486, 1651, 1730
SseBI AGGCCT 2 cut(s) 1584, 1649
SsiI CCGC 7 cut(s) 21, 101, 254, 698, 1427, 1504, 1687
SspI AATATT 1 cut(s) 607
SstI GAGCTC 1 cut(s) 1188
StuI AGGCCT 2 cut(s) 1584, 1649
StyD4I CCNGG 1 cut(s) 1258
StyI CCWWGG 2 cut(s) 763, 1053
TaaI ACNGT 2 cut(s) 214, 1693
TaiI ACGT 1 cut(s) 294
TaqI TCGA 4 cut(s) 305, 1063, 1139, 1158
TatI WGTACW 1 cut(s) 944
TauI GCSGC 2 cut(s) 23, 257
TfiI GAWTC 6 cut(s) 151, 307, 841, 1136, 1341, 1366
Tru1I TTAA 7 cut(s) 378, 515, 1248, 1271, 1553, 1652, 1673
Tru9I TTAA 7 cut(s) 378, 515, 1248, 1271, 1553, 1652, 1673
TscAI CASTG 2 cut(s) 1551, 1579
TseI GCWGC 5 cut(s) 316, 1058, 1388, 1459, 1495
TspDTI ATGAA 6 cut(s) 180, 503, 822, 846, 1023, 1194
TspGWI ACGGA 1 cut(s) 1677
TspRI CASTG 2 cut(s) 1551, 1579
Van91I CCANNNNNTGG 1 cut(s) 1337
Vha464I CTTAAG 2 cut(s) 377, 1651
VpaK11BI GGWCC 3 cut(s) 382, 703, 959
XagI CCTNNNNNAGG 1 cut(s) 698
XapI RAATTY 6 cut(s) 196, 353, 654, 1171, 1525, 1556
XbaI TCTAGA 2 cut(s) 310, 772
XceI RCATGY 1 cut(s) 819
XcmI CCANNNNNNNNNTGG 1 cut(s) 1128
XmaJI CCTAGG 1 cut(s) 763
XmiI GTMKAC 1 cut(s) 84
XmnI GAANNNNTTC 1 cut(s) 616
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.