Rh5BG238000

Leucine-rich repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003583
Physical Location & Seq
Forward (+)
28392356 .. 28400721
8366 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG238000.1

Sequence Viewer

Length: 1695 bp
ATGGATCGGGTCATCAAAGCCGCCAGAGCCTCCGGTTCTCTCAACCTTTCAAATCGCTCTCTCAGGTTCGTGCCCGATGAGGTATACAAAAGCGTGAATGCGGTTGGTGAAGACGAAAAGTGGTGGGAGGCTGTGGAGCTACACAAGCTGATTCTAGCTCATAATGAGATTGAATTGCTCAAGGATGAGCTAAGGAATTTGCCTCTACTGACTGTGTTGAATGTCAGCCACAATAAACTTTCCGAGCTTCCGGCGGCAATCGGAGAGCTTACCATGCTGAAATCGTTAGACGTGTCATTCAACTCGATTCTAGAGCTGCCTGAGGAAATTGGATCAGCAACTGCTCTTGTCAAATTTGATTGTTCAAATAATCAGCTTAAGGACCTCCCAAACTCACTTGGGAGATGCTCAGATCTATCAGATTTCAAGGCATCAAACAATGTTATCATCAGCTTGCCAGAAGATCTAGCAAACTGCTCAAAATTGATGAAATTGGATGTGGAGGGAAACAAGTTAACAAACCTATCTGAGAAGTTGATTGGATCGTGGACTTTGGTTACAGAACTCAATGCATCAAAAAATCTGCTGAGTGGAATACCAGAGAATATTGGAAGCCTTTCTCGTCTTATTCGTCTTGACCTTCATCAGAACAAAATTTCAGCGATCCCAGTTTCAATAAGGGGTTGTTGTTCCCTTGCGGAGGTCCACATGGGGAATAATGCACTCTCTACTTTACCATCAGAGATGGGGGAACTTTCTCGCCTAGGAACTCTAGATCTGCAATCAAACCAGTTGAAGGAGTATCCAGTGGAGGCATGTAAATTGCGTCTTTCATTATTGAATCTTTCAAACAATTCATTGTCTGGATTGCCCCCTGAATTGGGCAAGATGACTACCTTGCGAAGACTTTTACTTACCGGAAACCCTATACGAACTCTACGAAGTACATTGGTGTCTGGACCAACACCTAATTTATTGAAGTATCTGCGAAGTAGGCTTTCTCAGAATGAAGATGCTGAAGCTTCAACTGCAACAAAAGAGGATGTGATTGCCATGGCAGCTCGATTGTCTATTGCTTCAAAGGAACTTTCTATGGAAGGGCTGGGTTTGAGTTCTGTCCCATCAGAAGTATGGGAATCGAGTGAGGTCATAAAAGTCGATCTAACTAGGAATTCCATTGAAGAGCTCCCAATTGAACTTTCTTCATGTACCCACCTCCAGACACTAATCTTATCCAGAAACAAGATTAAAGAGTGGCCAGGTGCAATTTTAAGCTCGCTTCGTAATCTTTTGTGTTTGAAGCTGGACAATAATCCACTAAGACAGATACCATCAGATGGATTCCAAGCAACCCCCATGCTGCAGATTCTGGATCTAAGTGGTAATGCAGCTTCGTTACCAGAACACCCTGTATTTTCTAGCTTACCGCATCTGCTAGAGCTTTACCTGAGACGAATGCAGCTACGCGAAGTCCCATCAGATATCTTGAGTTTGCAGGAACTGCGGATCCTAGACCTTAGCCAAAATTCCCTGCAGTCAGTTCCAGTGGAGTTCAAAAATTTTACTTCACTCACTGAGCTAGGCCTATCTGACAACAACATATCAACACTTCCTCCAGAATTGGTCAGTATACGAGAACCTCTTGCTATTGAGTTGATCTTATATGTTGTGACTTCAACTGACTACAAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_1 LRR_5 LRR_8

Protein Analysis

564

Amino Acids

62.16

Weight (kDa)

5.32

Isoelectric Point (pI)

49.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 45 - 117 6.8e-07 Leucine-rich repeat region
LRR_8 PF13855 46 - 103 2.5e-07 Leucine rich repeat
LRR_14 PF23598 239 - 305 1.7e-06 Leucine-rich repeat region
LRR_14 PF23598 387 - 459 3.3e-06 Leucine-rich repeat region
LRR_8 PF13855 405 - 462 1.6e-09 Leucine rich repeat
LRR_14 PF23598 452 - 532 1.1e-06 Leucine-rich repeat region
LRR_8 PF13855 476 - 534 1.4e-08 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1337
AccI GTMKAC 2 cut(s) 84, 1630
AccII CGCG 1 cut(s) 1467
AciI CCGC 6 cut(s) 21, 101, 254, 698, 1427, 1504
AclWI GGATC 7 cut(s) 12, 340, 550, 658, 1380, 1501, 1514
AcoI YGGCCR 1 cut(s) 1256
AcsI RAATTY 6 cut(s) 196, 353, 654, 1171, 1525, 1558
AcuI CTGAAG 1 cut(s) 1038
AfaI GTAC 2 cut(s) 946, 1210
AfiI CCNNNNNNNGG 5 cut(s) 700, 796, 880, 881, 1337
AflII CTTAAG 1 cut(s) 377
AflIII ACRYGT 1 cut(s) 291
AjiI CACGTC 1 cut(s) 292
AjnI CCWGG 1 cut(s) 1258
AjuI GAANNNNNNNTTGG 2 cut(s) 1183, 1215
AloI GAACNNNNNNTCC 2 cut(s) 1491, 1523
Alw21I GWGCWC 1 cut(s) 1188
Alw26I GTCTC 1 cut(s) 1444
AlwI GGATC 7 cut(s) 12, 340, 550, 658, 1380, 1501, 1514
AlwNI CAGNNNCTG 3 cut(s) 341, 1369, 1501
AoxI GGCC 2 cut(s) 1256, 1582
ApeKI GCWGC 5 cut(s) 316, 1058, 1360, 1388, 1459
ApoI RAATTY 6 cut(s) 196, 353, 654, 1171, 1525, 1558
Asp700I GAANNNNTTC 1 cut(s) 616
AspA2I CCTAGG 1 cut(s) 763
AspS9I GGNCC 3 cut(s) 382, 703, 959
AsuHPI GGTGA 1 cut(s) 119
AvaII GGWCC 3 cut(s) 382, 703, 959
AvrII CCTAGG 1 cut(s) 763
AxyI CCTNAGG 1 cut(s) 321
BaeGI GKGCMC 1 cut(s) 75
BalI TGGCCA 1 cut(s) 1258
BamHI GGATCC 1 cut(s) 1506
BanII GRGCYC 1 cut(s) 1188
BarI GAAGNNNNNNTAC 2 cut(s) 1375, 1407
BbsI GAAGAC 2 cut(s) 117, 910
Bbv12I GWGCWC 1 cut(s) 1188
BbvI GCAGC 5 cut(s) 303, 1070, 1347, 1400, 1471
BccI CCATC 6 cut(s) 739, 745, 1129, 1331, 1339, 1483
BciT130I CCWGG 1 cut(s) 1260
BciVI GTATCC 1 cut(s) 813
BcoDI GTCTC 1 cut(s) 1444
BfmI CTRYAG 2 cut(s) 1361, 1532
BfrI CTTAAG 1 cut(s) 377
BfuI GTATCC 1 cut(s) 813
BglII AGATCT 3 cut(s) 412, 463, 775
BisI GCNGC 7 cut(s) 21, 255, 317, 1059, 1361, 1389, 1460
BlnI CCTAGG 1 cut(s) 763
BlsI GCNGC 7 cut(s) 22, 256, 318, 1060, 1362, 1390, 1461
Bme1390I CCNGG 1 cut(s) 1260
Bme18I GGWCC 3 cut(s) 382, 703, 959
BmgBI CACGTC 1 cut(s) 292
BmgT120I GGNCC 3 cut(s) 382, 703, 959
BmiI GGNNCC 1 cut(s) 1508
BmrFI CCNGG 1 cut(s) 1260
BmrI ACTGGG 1 cut(s) 662
BmsI GCATC 5 cut(s) 395, 440, 581, 1003, 1438
BmuI ACTGGG 1 cut(s) 662
BpiI GAAGAC 2 cut(s) 117, 910
BpmI CTGGAG 2 cut(s) 1202, 1599
Bpu10I CCTNAGC 2 cut(s) 191, 1517
BpuEI CTTGAG 2 cut(s) 164, 1507
BsaJI CCNNGG 2 cut(s) 763, 1053
BsaWI WCCGGW 2 cut(s) 32, 917
BsaXI ACNNNNNCTCC 2 cut(s) 1597, 1627
Bsc4I CCNNNNNNNGG 5 cut(s) 700, 796, 880, 881, 1337
Bse1I ACTGG 4 cut(s) 668, 790, 806, 1544
Bse21I CCTNAGG 1 cut(s) 321
BseBI CCWGG 1 cut(s) 1260
BseDI CCNNGG 2 cut(s) 763, 1053
BseGI GGATG 3 cut(s) 190, 502, 1048
BseLI CCNNNNNNNGG 5 cut(s) 700, 796, 880, 881, 1337
BseMII CTCAG 8 cut(s) 76, 312, 423, 519, 578, 1016, 1439, 1566
BseNI ACTGG 4 cut(s) 668, 790, 806, 1544
BseSI GKGCMC 1 cut(s) 75
BseXI GCAGC 5 cut(s) 303, 1070, 1347, 1400, 1471
BseYI CCCAGC 1 cut(s) 1102
Bsh1236I CGCG 1 cut(s) 1467
BshFI GGCC 2 cut(s) 1258, 1584
BsiHKAI GWGCWC 1 cut(s) 1188
BsiSI CCGG 3 cut(s) 33, 251, 918
BslFI GGGAC 2 cut(s) 1103, 1457
BslI CCNNNNNNNGG 5 cut(s) 700, 796, 880, 881, 1337
BsmAI GTCTC 1 cut(s) 1444
BsmBI CGTCTC 1 cut(s) 1444
BsmFI GGGAC 2 cut(s) 1103, 1457
BsmI GAATGC 2 cut(s) 103, 1461
BsnI GGCC 2 cut(s) 1258, 1584
Bsp1286I GDGCHC 2 cut(s) 75, 1188
Bsp19I CCATGG 1 cut(s) 1053
BspACI CCGC 6 cut(s) 21, 101, 254, 698, 1427, 1504
BspANI GGCC 2 cut(s) 1258, 1584
BspCNI CTCAG 8 cut(s) 75, 313, 422, 520, 579, 1015, 1440, 1567
BspFNI CGCG 1 cut(s) 1467
BspLI GGNNCC 1 cut(s) 1508
BspMAI CTGCAG 2 cut(s) 1365, 1536
BspPI GGATC 7 cut(s) 12, 340, 550, 658, 1380, 1501, 1514
BspQI GCTCTTC 1 cut(s) 1176
BspTI CTTAAG 1 cut(s) 377
BsrI ACTGG 4 cut(s) 668, 790, 806, 1544
BssECI CCNNGG 2 cut(s) 763, 1053
BssNAI GTATAC 2 cut(s) 85, 1631
BssT1I CCWWGG 2 cut(s) 763, 1053
Bst1107I GTATAC 2 cut(s) 85, 1631
Bst2UI CCWGG 1 cut(s) 1260
Bst4CI ACNGT 1 cut(s) 214
Bst6I CTCTTC 1 cut(s) 1176
BstAFI CTTAAG 1 cut(s) 377
BstAPI GCANNNNNTGC 1 cut(s) 1501
BstC8I GCNNGC 2 cut(s) 455, 1277
BstDSI CCRYGG 1 cut(s) 1053
BstENI CCTNNNNNAGG 1 cut(s) 698
BstF5I GGATG 3 cut(s) 190, 502, 1048
BstFNI CGCG 1 cut(s) 1467
BstMAI GTCTC 1 cut(s) 1444
BstMWI GCNNNNNNNGC 7 cut(s) 26, 145, 274, 994, 1028, 1058, 1501
BstNI CCWGG 1 cut(s) 1260
BstNSI RCATGY 1 cut(s) 819
BstSCI CCNGG 1 cut(s) 1258
BstSFI CTRYAG 2 cut(s) 1361, 1532
BstSLI GKGCMC 1 cut(s) 75
BstUI CGCG 1 cut(s) 1467
BstV1I GCAGC 5 cut(s) 303, 1070, 1347, 1400, 1471
BstV2I GAAGAC 2 cut(s) 117, 910
BstX2I RGATCY 5 cut(s) 412, 463, 775, 1372, 1506
BstYI RGATCY 5 cut(s) 412, 463, 775, 1372, 1506
BstZ17I GTATAC 2 cut(s) 85, 1631
Bsu36I CCTNAGG 1 cut(s) 321
BsuI GTATCC 1 cut(s) 813
BsuRI GGCC 2 cut(s) 1258, 1584
BtgI CCRYGG 1 cut(s) 1053
BtrI CACGTC 1 cut(s) 292
BtsCI GGATG 3 cut(s) 190, 502, 1048
BtsIMutI CAGTG 3 cut(s) 813, 1551, 1572
Cac8I GCNNGC 2 cut(s) 455, 1277
CaiI CAGNNNCTG 3 cut(s) 341, 1369, 1501
Cfr13I GGNCC 3 cut(s) 382, 703, 959
CseI GACGC 1 cut(s) 815
Csp6I GTAC 2 cut(s) 945, 1209
CviAII CATG 6 cut(s) 274, 709, 816, 1054, 1206, 1357
CviQI GTAC 2 cut(s) 945, 1209
EaeI YGGCCR 1 cut(s) 1256
Eam1104I CTCTTC 1 cut(s) 1176
EarI CTCTTC 1 cut(s) 1176
Ecl136II GAGCTC 1 cut(s) 1186
Eco130I CCWWGG 2 cut(s) 763, 1053
Eco147I AGGCCT 1 cut(s) 1584
Eco24I GRGCYC 1 cut(s) 1188
Eco32I GATATC 1 cut(s) 1483
Eco47I GGWCC 3 cut(s) 382, 703, 959
Eco53kI GAGCTC 1 cut(s) 1186
Eco57I CTGAAG 1 cut(s) 1038
Eco81I CCTNAGG 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 1186
EcoNI CCTNNNNNAGG 1 cut(s) 698
EcoO109I RGGNCCY 1 cut(s) 382
EcoRI GAATTC 1 cut(s) 1171
EcoRII CCWGG 1 cut(s) 1258
EcoRV GATATC 1 cut(s) 1483
EcoT14I CCWWGG 2 cut(s) 763, 1053
EcoT22I ATGCAT 1 cut(s) 574
EcoT38I GRGCYC 1 cut(s) 1188
ErhI CCWWGG 2 cut(s) 763, 1053
Esp3I CGTCTC 1 cut(s) 1444
FaeI CATG 6 cut(s) 277, 712, 819, 1057, 1209, 1360
FaqI GGGAC 2 cut(s) 1103, 1457
FatI CATG 6 cut(s) 273, 708, 815, 1053, 1205, 1356
FblI GTMKAC 2 cut(s) 84, 1630
Fnu4HI GCNGC 7 cut(s) 21, 255, 317, 1059, 1361, 1389, 1460
FokI GGATG 3 cut(s) 197, 509, 1055
FriOI GRGCYC 1 cut(s) 1188
Fsp4HI GCNGC 7 cut(s) 21, 255, 317, 1059, 1361, 1389, 1460
GluI GCNGC 7 cut(s) 21, 255, 317, 1059, 1361, 1389, 1460
GsaI CCCAGC 1 cut(s) 1106
GsuI CTGGAG 2 cut(s) 1202, 1599
HaeIII GGCC 2 cut(s) 1258, 1584
HapII CCGG 3 cut(s) 33, 251, 918
HgaI GACGC 1 cut(s) 815
Hin1II CATG 6 cut(s) 277, 712, 819, 1057, 1209, 1360
HincII GTYRAC 1 cut(s) 516
HindII GTYRAC 1 cut(s) 516
HindIII AAGCTT 1 cut(s) 1020
HinfI GANTC 6 cut(s) 151, 307, 841, 1136, 1341, 1366
HpaI GTTAAC 1 cut(s) 516
HpaII CCGG 3 cut(s) 33, 251, 918
HphI GGTGA 1 cut(s) 119
Hpy166II GTNNAC 5 cut(s) 85, 516, 549, 706, 1631
Hpy8I GTNNAC 5 cut(s) 85, 516, 549, 706, 1631
HpyAV CCTTC 3 cut(s) 650, 790, 1091
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4IV ACGT 1 cut(s) 291
HpyF10VI GCNNNNNNNGC 7 cut(s) 26, 145, 274, 994, 1028, 1058, 1501
HpySE526I ACGT 1 cut(s) 291
Hsp92II CATG 6 cut(s) 277, 712, 819, 1057, 1209, 1360
KspAI GTTAAC 1 cut(s) 516
LguI GCTCTTC 1 cut(s) 1176
LmnI GCTCC 2 cut(s) 136, 1191
Lsp1109I GCAGC 5 cut(s) 303, 1070, 1347, 1400, 1471
LweI GCATC 5 cut(s) 395, 440, 581, 1003, 1438
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 3 cut(s) 556, 1395, 1669
MboII GAAGA 6 cut(s) 122, 473, 915, 1022, 1193, 1194
MfeI CAATTG 1 cut(s) 1191
MflI RGATCY 5 cut(s) 412, 463, 775, 1372, 1506
MhlI GDGCHC 2 cut(s) 75, 1188
MlsI TGGCCA 1 cut(s) 1258
MluNI TGGCCA 1 cut(s) 1258
Mox20I TGGCCA 1 cut(s) 1258
Mph1103I ATGCAT 1 cut(s) 574
MroXI GAANNNNTTC 1 cut(s) 616
MscI TGGCCA 1 cut(s) 1258
MseI TTAA 4 cut(s) 378, 515, 1248, 1271
Msp20I TGGCCA 1 cut(s) 1258
MspCI CTTAAG 1 cut(s) 377
MspI CCGG 3 cut(s) 33, 251, 918
MspR9I CCNGG 1 cut(s) 1260
MunI CAATTG 1 cut(s) 1191
Mva1269I GAATGC 2 cut(s) 103, 1461
MvaI CCWGG 1 cut(s) 1260
MvnI CGCG 1 cut(s) 1467
MwoI GCNNNNNNNGC 7 cut(s) 26, 145, 274, 994, 1028, 1058, 1501
NcoI CCATGG 1 cut(s) 1053
NlaIII CATG 6 cut(s) 277, 712, 819, 1057, 1209, 1360
NlaIV GGNNCC 1 cut(s) 1508
NmuCI GTSAC 1 cut(s) 1669
NsiI ATGCAT 1 cut(s) 574
NspI RCATGY 1 cut(s) 819
PceI AGGCCT 1 cut(s) 1584
PciSI GCTCTTC 1 cut(s) 1176
PcsI WCGNNNNNNNCGW 2 cut(s) 628, 937
PctI GAATGC 2 cut(s) 103, 1461
PdmI GAANNNNTTC 1 cut(s) 616
PfeI GAWTC 6 cut(s) 151, 307, 841, 1136, 1341, 1366
PflMI CCANNNNNTGG 1 cut(s) 1337
PkrI GCNGC 7 cut(s) 22, 256, 318, 1060, 1362, 1390, 1461
PpuMI RGGWCCY 1 cut(s) 382
Psp124BI GAGCTC 1 cut(s) 1188
Psp5II RGGWCCY 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 1258
PspFI CCCAGC 1 cut(s) 1102
PspGI CCWGG 1 cut(s) 1258
PspN4I GGNNCC 1 cut(s) 1508
PspPI GGNCC 3 cut(s) 382, 703, 959
PspPPI RGGWCCY 1 cut(s) 382
PstI CTGCAG 2 cut(s) 1365, 1536
PstNI CAGNNNCTG 3 cut(s) 341, 1369, 1501
PsuI RGATCY 5 cut(s) 412, 463, 775, 1372, 1506
RsaI GTAC 2 cut(s) 946, 1210
RsaNI GTAC 2 cut(s) 945, 1209
SacI GAGCTC 1 cut(s) 1188
SapI GCTCTTC 1 cut(s) 1176
SaqAI TTAA 4 cut(s) 378, 515, 1248, 1271
SatI GCNGC 7 cut(s) 21, 255, 317, 1059, 1361, 1389, 1460
Sau96I GGNCC 3 cut(s) 382, 703, 959
ScrFI CCNGG 1 cut(s) 1260
SduI GDGCHC 2 cut(s) 75, 1188
SfaNI GCATC 5 cut(s) 395, 440, 581, 1003, 1438
SfcI CTRYAG 2 cut(s) 1361, 1532
SinI GGWCC 3 cut(s) 382, 703, 959
SmlI CTYRAG 3 cut(s) 179, 377, 1486
SmoI CTYRAG 3 cut(s) 179, 377, 1486
SseBI AGGCCT 1 cut(s) 1584
SsiI CCGC 6 cut(s) 21, 101, 254, 698, 1427, 1504
SspI AATATT 1 cut(s) 607
SstI GAGCTC 1 cut(s) 1188
StuI AGGCCT 1 cut(s) 1584
StyD4I CCNGG 1 cut(s) 1258
StyI CCWWGG 2 cut(s) 763, 1053
TaaI ACNGT 1 cut(s) 214
TaiI ACGT 1 cut(s) 294
TaqI TCGA 4 cut(s) 305, 1063, 1139, 1158
TatI WGTACW 1 cut(s) 944
TauI GCSGC 2 cut(s) 23, 257
TfiI GAWTC 6 cut(s) 151, 307, 841, 1136, 1341, 1366
Tru1I TTAA 4 cut(s) 378, 515, 1248, 1271
Tru9I TTAA 4 cut(s) 378, 515, 1248, 1271
TscAI CASTG 3 cut(s) 813, 1551, 1579
TseFI GTSAC 1 cut(s) 1669
TseI GCWGC 5 cut(s) 316, 1058, 1360, 1388, 1459
Tsp45I GTSAC 1 cut(s) 1669
TspDTI ATGAA 6 cut(s) 503, 632, 822, 846, 1023, 1194
TspRI CASTG 3 cut(s) 813, 1551, 1579
Van91I CCANNNNNTGG 1 cut(s) 1337
Vha464I CTTAAG 1 cut(s) 377
VpaK11BI GGWCC 3 cut(s) 382, 703, 959
XagI CCTNNNNNAGG 1 cut(s) 698
XapI RAATTY 6 cut(s) 196, 353, 654, 1171, 1525, 1558
XbaI TCTAGA 2 cut(s) 310, 772
XceI RCATGY 1 cut(s) 819
XcmI CCANNNNNNNNNTGG 1 cut(s) 1128
XmaJI CCTAGG 1 cut(s) 763
XmiI GTMKAC 2 cut(s) 84, 1630
XmnI GAANNNNTTC 1 cut(s) 616
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.