RchiOBHm_Chr5g0045871

amine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006136
Physical Location & Seq
Forward (+)
41895729 .. 41897326
1598 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32392

Sequence Viewer

Length: 723 bp
ATGAAGATCACAATGTACACTGATAGGTTCATAGCCCCTTTGCCTAAAGCTGAAGGCACTGATTTCCAAACCCCAAATGGGAAGTCAAATTATGATCACTGCAACAAAACTAGCGGATTTTCCATTAAAGACCACAAGGTGAAGTGGGCAAACTGGGTTTTTCATGTTGGGTTCAAGGCTAGAGCAGGAATGAGGGCATGTGTCTATGAGACCTTTGTGCCTTACATGGATCCTCCCAATGAATGGTATTTCAGGACTTTTATGGATATAGGAGAATTTGGGTTTGGGAGATCAGCTGACGCTCTTCAGCCATTAATTGATTGTCCCGGAAATGCTGAATATGTAGATGGGTTTATGGCTGGGGCTGATGGGGAGGTACAGAAGGTACCAAGGGCTATTTGCATTTTTGAATTGTACTCAGGTGATATTACAATGAGACATACTGAAATTAATGTACCTAGCAAATTGATAAGAAGTGGGCAGCAGGAGAAGACTCTAGTGGTTAGAATGGAGGCTACTGTGGGTAACTATGATTATGTTCTTGATTGGGAATTCAAGCAAAGTGGCACCACTAAAGTTGGGTTAATGTCCTTGGAGGTGAAGGCCACGTCATACACAAATGCTGACCAGATGACGGAAAACGTGCACGGAATGTTAGTCTCAAAAAACACTCTTGCCGTTAACCACGACCACTTCCTCACGTACTATCTAGATCTACAGTAA

Protein Analysis

240

Amino Acids

27.25

Weight (kDa)

5.8

Isoelectric Point (pI)

26.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_amine_oxid PF01179 38 - 239 3.1e-54 Copper amine oxidase, enzyme domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 385
AccB1I GGYRCC 2 cut(s) 385, 566
AccB7I CCANNNNNTGG 1 cut(s) 243
AciI CCGC 1 cut(s) 114
AclWI GGATC 2 cut(s) 224, 237
AcsI RAATTY 2 cut(s) 275, 551
AcuI CTGAAG 2 cut(s) 72, 290
AdeI CACNNNGTG 1 cut(s) 139
AfaI GTAC 6 cut(s) 17, 378, 387, 416, 456, 704
AfiI CCNNNNNNNGG 3 cut(s) 78, 243, 634
AgsI TTSAA 3 cut(s) 175, 410, 556
AjiI CACGTC 1 cut(s) 609
AjuI GAANNNNNNNTTGG 2 cut(s) 267, 299
AluBI AGCT 2 cut(s) 50, 296
AluI AGCT 2 cut(s) 50, 296
Alw21I GWGCWC 1 cut(s) 648
Alw26I GTCTC 3 cut(s) 203, 430, 664
Alw44I GTGCAC 1 cut(s) 644
AlwI GGATC 2 cut(s) 224, 237
AoxI GGCC 1 cut(s) 603
ApaLI GTGCAC 1 cut(s) 644
ApeKI GCWGC 1 cut(s) 481
ApoI RAATTY 2 cut(s) 275, 551
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
AseI ATTAAT 2 cut(s) 314, 450
Asp718I GGTACC 1 cut(s) 385
AsuC2I CCSGG 1 cut(s) 327
AsuHPI GGTGA 3 cut(s) 151, 434, 610
BaeGI GKGCMC 1 cut(s) 648
BamHI GGATCC 1 cut(s) 229
BanI GGYRCC 2 cut(s) 385, 566
BbsI GAAGAC 1 cut(s) 497
Bbv12I GWGCWC 1 cut(s) 648
BbvI GCAGC 1 cut(s) 493
BccI CCATC 2 cut(s) 341, 362
BceAI ACGGC 1 cut(s) 662
BclI TGATCA 1 cut(s) 94
BcnI CCSGG 1 cut(s) 327
BcoDI GTCTC 3 cut(s) 203, 430, 664
BfaI CTAG 5 cut(s) 111, 180, 459, 497, 710
BfmI CTRYAG 1 cut(s) 716
BglII AGATCT 1 cut(s) 712
BisI GCNGC 1 cut(s) 482
BlsI GCNGC 1 cut(s) 483
Bme1390I CCNGG 1 cut(s) 327
BmgBI CACGTC 1 cut(s) 609
BmiI GGNNCC 3 cut(s) 231, 387, 568
BmrFI CCNGG 1 cut(s) 327
BmrI ACTGGG 1 cut(s) 163
BmuI ACTGGG 1 cut(s) 163
BpiI GAAGAC 1 cut(s) 497
BpuMI CCSGG 1 cut(s) 327
BsaAI YACGTR 1 cut(s) 702
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 2 cut(s) 389, 591
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 243, 634
Bse1I ACTGG 1 cut(s) 158
BseDI CCNNGG 2 cut(s) 389, 591
BseLI CCNNNNNNNGG 3 cut(s) 78, 243, 634
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 1 cut(s) 158
BseSI GKGCMC 1 cut(s) 648
BseXI GCAGC 1 cut(s) 493
BseYI CCCAGC 1 cut(s) 359
BshFI GGCC 1 cut(s) 605
BshNI GGYRCC 2 cut(s) 385, 566
BsiHKAI GWGCWC 1 cut(s) 648
BsiSI CCGG 1 cut(s) 327
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 3 cut(s) 78, 243, 634
BsmAI GTCTC 3 cut(s) 203, 430, 664
BsmFI GGGAC 1 cut(s) 309
BsnI GGCC 1 cut(s) 605
Bso31I GGTCTC 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 648
Bsp1407I TGTACA 1 cut(s) 15
Bsp143I GATC 5 cut(s) 6, 94, 229, 290, 712
BspACI CCGC 1 cut(s) 114
BspANI GGCC 1 cut(s) 605
BspCNI CTCAG 1 cut(s) 431
BspLI GGNNCC 3 cut(s) 231, 387, 568
BspPI GGATC 2 cut(s) 224, 237
BspQI GCTCTTC 1 cut(s) 309
BspT107I GGYRCC 2 cut(s) 385, 566
BspTNI GGTCTC 1 cut(s) 203
BsrGI TGTACA 1 cut(s) 15
BsrI ACTGG 1 cut(s) 158
BssECI CCNNGG 2 cut(s) 389, 591
BssMI GATC 5 cut(s) 6, 94, 229, 290, 712
BssT1I CCWWGG 2 cut(s) 389, 591
Bst4CI ACNGT 2 cut(s) 520, 720
Bst6I CTCTTC 1 cut(s) 309
BstAUI TGTACA 1 cut(s) 15
BstBAI YACGTR 1 cut(s) 702
BstDEI CTNAG 1 cut(s) 418
BstKTI GATC 5 cut(s) 9, 97, 232, 293, 715
BstMAI GTCTC 3 cut(s) 203, 430, 664
BstMBI GATC 5 cut(s) 6, 94, 229, 290, 712
BstNSI RCATGY 1 cut(s) 201
BstSCI CCNGG 1 cut(s) 325
BstSFI CTRYAG 1 cut(s) 716
BstSLI GKGCMC 1 cut(s) 648
BstV1I GCAGC 1 cut(s) 493
BstV2I GAAGAC 1 cut(s) 497
BstX2I RGATCY 2 cut(s) 229, 712
BstYI RGATCY 2 cut(s) 229, 712
BsuRI GGCC 1 cut(s) 605
BtrI CACGTC 1 cut(s) 609
BtsI GCAGTG 1 cut(s) 97
BtsIMutI CAGTG 3 cut(s) 18, 57, 97
CseI GACGC 1 cut(s) 308
Csp6I GTAC 6 cut(s) 16, 377, 386, 415, 455, 703
CspCI CAANNNNNGTGG 4 cut(s) 544, 559, 579, 594
CviAII CATG 3 cut(s) 164, 198, 226
CviQI GTAC 6 cut(s) 16, 377, 386, 415, 455, 703
DdeI CTNAG 1 cut(s) 418
DpnI GATC 5 cut(s) 8, 96, 231, 292, 714
DpnII GATC 5 cut(s) 6, 94, 229, 290, 712
DraIII CACNNNGTG 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 309
EarI CTCTTC 1 cut(s) 309
Eco130I CCWWGG 2 cut(s) 389, 591
Eco31I GGTCTC 1 cut(s) 203
Eco57I CTGAAG 2 cut(s) 72, 290
EcoRI GAATTC 1 cut(s) 551
EcoT14I CCWWGG 2 cut(s) 389, 591
ErhI CCWWGG 2 cut(s) 389, 591
FaeI CATG 3 cut(s) 167, 201, 229
FaqI GGGAC 1 cut(s) 309
FatI CATG 3 cut(s) 163, 197, 225
FbaI TGATCA 1 cut(s) 94
Fnu4HI GCNGC 1 cut(s) 482
Fsp4HI GCNGC 1 cut(s) 482
FspBI CTAG 5 cut(s) 111, 180, 459, 497, 710
GluI GCNGC 1 cut(s) 482
GsaI CCCAGC 1 cut(s) 363
HaeIII GGCC 1 cut(s) 605
HapII CCGG 1 cut(s) 327
HgaI GACGC 1 cut(s) 308
Hin1II CATG 3 cut(s) 167, 201, 229
HincII GTYRAC 1 cut(s) 682
HindII GTYRAC 1 cut(s) 682
HinfI GANTC 1 cut(s) 493
HpaI GTTAAC 1 cut(s) 682
HpaII CCGG 1 cut(s) 327
HphI GGTGA 3 cut(s) 151, 434, 610
Hpy166II GTNNAC 3 cut(s) 18, 646, 682
Hpy188III TCNNGA 3 cut(s) 253, 542, 710
Hpy8I GTNNAC 3 cut(s) 18, 646, 682
HpyAV CCTTC 3 cut(s) 47, 376, 595
HpyCH4III ACNGT 2 cut(s) 520, 720
HpyCH4IV ACGT 3 cut(s) 608, 642, 701
HpyCH4V TGCA 3 cut(s) 102, 402, 646
HpyF3I CTNAG 1 cut(s) 418
HpySE526I ACGT 3 cut(s) 608, 642, 701
Hsp92II CATG 3 cut(s) 167, 201, 229
KpnI GGTACC 1 cut(s) 389
Ksp22I TGATCA 1 cut(s) 94
KspAI GTTAAC 1 cut(s) 682
Kzo9I GATC 5 cut(s) 6, 94, 229, 290, 712
LguI GCTCTTC 1 cut(s) 309
LpnPI CCDG 8 cut(s) 139, 171, 238, 340, 345, 405, 470, 641
Lsp1109I GCAGC 1 cut(s) 493
MaeI CTAG 5 cut(s) 111, 180, 459, 497, 710
MaeII ACGT 3 cut(s) 608, 642, 701
MaeIII GTNAC 1 cut(s) 524
MalI GATC 5 cut(s) 8, 96, 231, 292, 714
MboI GATC 5 cut(s) 6, 94, 229, 290, 712
MboII GAAGA 3 cut(s) 16, 296, 502
MflI RGATCY 2 cut(s) 229, 712
MhlI GDGCHC 1 cut(s) 648
MluCI AATT 7 cut(s) 88, 275, 315, 410, 447, 464, 551
MlyI GAGTC 1 cut(s) 487
MnlI CCTC 6 cut(s) 186, 243, 367, 505, 589, 707
MseI TTAA 5 cut(s) 126, 314, 450, 584, 681
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 1 cut(s) 327
NciI CCSGG 1 cut(s) 327
NdeII GATC 5 cut(s) 6, 94, 229, 290, 712
NlaIII CATG 3 cut(s) 167, 201, 229
NlaIV GGNNCC 3 cut(s) 231, 387, 568
NspI RCATGY 1 cut(s) 201
PciSI GCTCTTC 1 cut(s) 309
PflMI CCANNNNNTGG 1 cut(s) 243
PfoI TCCNGGA 1 cut(s) 325
PkrI GCNGC 1 cut(s) 483
PleI GAGTC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 487
Ppu21I YACGTR 1 cut(s) 702
PshBI ATTAAT 2 cut(s) 314, 450
PspFI CCCAGC 1 cut(s) 359
PspN4I GGNNCC 3 cut(s) 231, 387, 568
PsuI RGATCY 2 cut(s) 229, 712
PvuII CAGCTG 1 cut(s) 296
RsaI GTAC 6 cut(s) 17, 378, 387, 416, 456, 704
RsaNI GTAC 6 cut(s) 16, 377, 386, 415, 455, 703
SapI GCTCTTC 1 cut(s) 309
SaqAI TTAA 5 cut(s) 126, 314, 450, 584, 681
SatI GCNGC 1 cut(s) 482
Sau3AI GATC 5 cut(s) 6, 94, 229, 290, 712
SchI GAGTC 1 cut(s) 487
ScrFI CCNGG 1 cut(s) 327
SduI GDGCHC 1 cut(s) 648
SfcI CTRYAG 1 cut(s) 716
Sse9I AATT 7 cut(s) 88, 275, 315, 410, 447, 464, 551
SsiI CCGC 1 cut(s) 114
SspMI CTAG 5 cut(s) 111, 180, 459, 497, 710
StyD4I CCNGG 1 cut(s) 325
StyI CCWWGG 2 cut(s) 389, 591
TaaI ACNGT 2 cut(s) 520, 720
TaiI ACGT 3 cut(s) 611, 645, 704
TasI AATT 7 cut(s) 88, 275, 315, 410, 447, 464, 551
TatI WGTACW 2 cut(s) 15, 414
Tru1I TTAA 5 cut(s) 126, 314, 450, 584, 681
Tru9I TTAA 5 cut(s) 126, 314, 450, 584, 681
TscAI CASTG 3 cut(s) 25, 64, 104
TseI GCWGC 1 cut(s) 481
TspDTI ATGAA 4 cut(s) 17, 19, 152, 255
TspGWI ACGGA 2 cut(s) 650, 663
TspRI CASTG 3 cut(s) 25, 64, 104
Van91I CCANNNNNTGG 1 cut(s) 243
VneI GTGCAC 1 cut(s) 644
VspI ATTAAT 2 cut(s) 314, 450
XapI RAATTY 2 cut(s) 275, 551
XbaI TCTAGA 1 cut(s) 709
XceI RCATGY 1 cut(s) 201
XcmI CCANNNNNNNNNTGG 1 cut(s) 74
XspI CTAG 5 cut(s) 111, 180, 459, 497, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.