RchiOBHm_Chr5g0050071

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013759
Physical Location & Seq
Forward (+)
48516656 .. 48518136
1481 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32770

Sequence Viewer

Length: 1392 bp
ATGTTGAAAGCTAGAGCTACACTTCCCATTGCTGGTTTGAAGGGTGATATATTGCAATTGTTGCAGGATAATAATGTTCAAGTAGTCTGTGGGGAAACGGGATCAGGAAAAACAACTCAGGTTCCACAATTTATATTGGATGACATGATCCAGTCAGGACGTGGTGGCCCCTGTAACATTATATGCACACAACCATGGAGAATAGCGGCTATTTCTGTGGCCGATAGAGTCGCTGATGAGCGTTGTGAACCTTCACCTGGTTCAAATGGTTCTTTGGTTGGTTATCAAGTTCGCCTTCATAGTGCAAGTAATGAAAAGACTAAGCTTCTGTTTTGCACGACAGGTATTCTTCTGAGAAAATTTGTGGGAGACAGAAACTTGACTGGAATTACTCATGTCATAGTTGATGAAGTACACAAGCGGTCTCTTTTGGGTGATTACCTACTCATAGTTTTGAAGAATCTGATTGAAATTGGAAATCCTTATGCCTTGCTTTTGGCTCAGAAAATGGGAGAAAAAGTTGATGTTCTGTTTTTTCTCGTGCCGAGGTGGCTTGCTGCTTATATACCAATTTGTTTGTCTTGCAAAGTTGTTAAGTGCATTACTGCTGTGATTGCCATTCCTGTGATATTGGTTTGTGCTGTGTTTCTAACTGCAGTTTATGTCCGCTACAAGTTACGTGACTGTCGTTGCACAGAAATAGACTTGAAGTCTACAAAGAATGGCCGCAGTGGTTCTCTTTTCCAGAAAGACCTTGGCAAGATTCATCATGTTGCTTCAATAATGGTGACAAGAGATGTGGCTTCAAGAGAGTTTGATATCACTCATGTTGCACAGGTCGTCAATTTTGACTTTTCAAAAATCATAGATGATTATTTTCACAGGATTGGTCATCCTGGTATGGTAGTGGAGTTCATAAAGGAATCCAAAAACGAGGTTCCTAATTGGCTTATTAATCATGCTCAACAATCGTTGTACTATGGTGGAGGCCAAAGCCAAGGTTATACATGTCTCTTGAATCACAGAATGGAGTTCCTTTGCATGCCACATAGTGAGATAGCCATCTTTTGGATTTGGCTTCCTACACCACCACTACGTTTAAAGGTTTGGGTACTTAACCGGCATGGTTTGATTGTGATCAAGGCTCAAATGTTAATTGGTGATGCATTCTTGAAAATAGAGCTCATGTTTGATAATGAATCTCACATCATTTCCACGGGGGCAATTGCTATTAGACTTGTTTGTCATTTTGGTACAGACTTGCAGAAAGAAAGCCCTTCCACTTGCCGGCAAGGGAAAACTCAAAGAAATGGCTGCATTATTCAACTGGTTCTTGTGTGCAAGTTCAGAATTTGCCACTTTGGCCACTCTAAATGCATGGTTGAATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016282 GO:0032991 GO:0043021 GO:0043024 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0070993 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

463

Amino Acids

51.87

Weight (kDa)

8.89

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 20 - 140 1.2e-06 DEAD/DEAH box helicase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1242
AccB7I CCANNNNNTGG 1 cut(s) 1068
AccI GTMKAC 1 cut(s) 713
AciI CCGC 4 cut(s) 206, 421, 667, 727
AclWI GGATC 2 cut(s) 109, 142
AcoI YGGCCR 3 cut(s) 219, 724, 1363
AcsI RAATTY 2 cut(s) 359, 1350
AdeI CACNNNGTG 1 cut(s) 1052
AfaI GTAC 4 cut(s) 414, 977, 1113, 1255
AfiI CCNNNNNNNGG 4 cut(s) 32, 257, 1068, 1287
AflIII ACRYGT 1 cut(s) 1007
AhdI GACNNNNNGTC 1 cut(s) 709
AjiI CACGTC 1 cut(s) 161
AjnI CCWGG 2 cut(s) 256, 895
AluBI AGCT 4 cut(s) 11, 17, 325, 1183
AluI AGCT 4 cut(s) 11, 17, 325, 1183
Alw21I GWGCWC 1 cut(s) 1185
Alw26I GTCTC 3 cut(s) 363, 429, 1016
AlwI GGATC 2 cut(s) 109, 142
AoxI GGCC 5 cut(s) 166, 219, 724, 988, 1363
ApeKI GCWGC 2 cut(s) 557, 1314
ApoI RAATTY 2 cut(s) 359, 1350
AseI ATTAAT 1 cut(s) 954
AspS9I GGNCC 1 cut(s) 167
AsuHPI GGTGA 5 cut(s) 56, 246, 446, 799, 1172
BalI TGGCCA 1 cut(s) 1365
BanII GRGCYC 1 cut(s) 1185
BauI CACGAG 1 cut(s) 539
Bbv12I GWGCWC 1 cut(s) 1185
BbvI GCAGC 2 cut(s) 544, 1301
BccI CCATC 1 cut(s) 1070
BciT130I CCWGG 2 cut(s) 258, 897
BclI TGATCA 1 cut(s) 1137
BcoDI GTCTC 3 cut(s) 363, 429, 1016
BfaI CTAG 1 cut(s) 12
BfmI CTRYAG 1 cut(s) 654
BglI GCCNNNNNGGC 2 cut(s) 550, 1362
BisI GCNGC 4 cut(s) 207, 558, 727, 1315
BlsI GCNGC 4 cut(s) 208, 559, 728, 1316
Bme1390I CCNGG 2 cut(s) 258, 897
BmeRI GACNNNNNGTC 1 cut(s) 709
BmgBI CACGTC 1 cut(s) 161
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 3 cut(s) 123, 169, 939
BmrFI CCNGG 2 cut(s) 258, 897
BmsI GCATC 1 cut(s) 1153
BsaAI YACGTR 1 cut(s) 680
BsaBI GATNNNNATC 2 cut(s) 1061, 1136
BsaI GGTCTC 1 cut(s) 429
BsaJI CCNNGG 5 cut(s) 194, 545, 754, 997, 1215
BsaXI ACNNNNNCTCC 2 cut(s) 504, 534
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 257, 1068, 1287
Bse118I RCCGGY 2 cut(s) 1119, 1287
Bse1I ACTGG 3 cut(s) 151, 388, 1332
Bse3DI GCAATG 1 cut(s) 27
Bse8I GATNNNNATC 2 cut(s) 1061, 1136
BseBI CCWGG 2 cut(s) 258, 897
BseDI CCNNGG 5 cut(s) 194, 545, 754, 997, 1215
BseGI GGATG 2 cut(s) 145, 892
BseJI GATNNNNATC 2 cut(s) 1061, 1136
BseLI CCNNNNNNNGG 4 cut(s) 32, 257, 1068, 1287
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 3 cut(s) 131, 344, 515
BseNI ACTGG 3 cut(s) 151, 388, 1332
BseXI GCAGC 2 cut(s) 544, 1301
BshFI GGCC 5 cut(s) 168, 221, 726, 990, 1365
BsiHKAI GWGCWC 1 cut(s) 1185
BsiSI CCGG 2 cut(s) 1120, 1288
BslI CCNNNNNNNGG 4 cut(s) 32, 257, 1068, 1287
BsmAI GTCTC 3 cut(s) 363, 429, 1016
BsmI GAATGC 1 cut(s) 1166
BsnI GGCC 5 cut(s) 168, 221, 726, 990, 1365
Bso31I GGTCTC 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 1185
Bsp143I GATC 3 cut(s) 101, 147, 1137
Bsp19I CCATGG 1 cut(s) 194
BspACI CCGC 4 cut(s) 206, 421, 667, 727
BspANI GGCC 5 cut(s) 168, 221, 726, 990, 1365
BspCNI CTCAG 3 cut(s) 130, 345, 514
BspLI GGNNCC 3 cut(s) 123, 169, 939
BspMAI CTGCAG 1 cut(s) 658
BspPI GGATC 2 cut(s) 109, 142
BspTNI GGTCTC 1 cut(s) 429
BsrDI GCAATG 1 cut(s) 27
BsrFI RCCGGY 2 cut(s) 1119, 1287
BsrI ACTGG 3 cut(s) 151, 388, 1332
BssAI RCCGGY 2 cut(s) 1119, 1287
BssECI CCNNGG 5 cut(s) 194, 545, 754, 997, 1215
BssMI GATC 3 cut(s) 101, 147, 1137
BssSI CACGAG 1 cut(s) 539
BssT1I CCWWGG 3 cut(s) 194, 754, 997
Bst2BI CACGAG 1 cut(s) 539
Bst2UI CCWGG 2 cut(s) 258, 897
Bst4CI ACNGT 1 cut(s) 686
BstAPI GCANNNNNTGC 1 cut(s) 61
BstBAI YACGTR 1 cut(s) 680
BstC8I GCNNGC 3 cut(s) 555, 1043, 1289
BstDEI CTNAG 4 cut(s) 117, 321, 353, 501
BstDSI CCRYGG 2 cut(s) 194, 1215
BstF5I GGATG 2 cut(s) 145, 892
BstKTI GATC 3 cut(s) 104, 150, 1140
BstMAI GTCTC 3 cut(s) 363, 429, 1016
BstMBI GATC 3 cut(s) 101, 147, 1137
BstMWI GCNNNNNNNGC 4 cut(s) 61, 550, 614, 1362
BstNI CCWGG 2 cut(s) 258, 897
BstNSI RCATGY 2 cut(s) 1011, 1045
BstSCI CCNGG 2 cut(s) 256, 895
BstSFI CTRYAG 1 cut(s) 654
BstV1I GCAGC 2 cut(s) 544, 1301
BsuRI GGCC 5 cut(s) 168, 221, 726, 990, 1365
BtgI CCRYGG 2 cut(s) 194, 1215
BtrI CACGTC 1 cut(s) 161
BtsCI GGATG 2 cut(s) 145, 892
BtsI GCAGTG 1 cut(s) 736
BtsIMutI CAGTG 1 cut(s) 736
Cac8I GCNNGC 3 cut(s) 555, 1043, 1289
Cfr10I RCCGGY 2 cut(s) 1119, 1287
Cfr13I GGNCC 1 cut(s) 167
CsiI ACCWGGT 1 cut(s) 256
Csp6I GTAC 4 cut(s) 413, 976, 1112, 1254
CspCI CAANNNNNGTGG 2 cut(s) 780, 815
CviQI GTAC 4 cut(s) 413, 976, 1112, 1254
DdeI CTNAG 4 cut(s) 117, 321, 353, 501
DpnI GATC 3 cut(s) 103, 149, 1139
DpnII GATC 3 cut(s) 101, 147, 1137
DraI TTTAAA 1 cut(s) 1101
DraIII CACNNNGTG 1 cut(s) 1052
DrdI GACNNNNNNGTC 1 cut(s) 1242
DriI GACNNNNNGTC 1 cut(s) 709
DseDI GACNNNNNNGTC 1 cut(s) 1242
EaeI YGGCCR 3 cut(s) 219, 724, 1363
Eam1105I GACNNNNNGTC 1 cut(s) 709
Ecl136II GAGCTC 1 cut(s) 1183
Eco130I CCWWGG 3 cut(s) 194, 754, 997
Eco24I GRGCYC 1 cut(s) 1185
Eco31I GGTCTC 1 cut(s) 429
Eco32I GATATC 1 cut(s) 820
Eco53kI GAGCTC 1 cut(s) 1183
EcoICRI GAGCTC 1 cut(s) 1183
EcoRII CCWGG 2 cut(s) 256, 895
EcoRV GATATC 1 cut(s) 820
EcoT14I CCWWGG 3 cut(s) 194, 754, 997
EcoT22I ATGCAT 2 cut(s) 1168, 1379
EcoT38I GRGCYC 1 cut(s) 1185
ErhI CCWWGG 3 cut(s) 194, 754, 997
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FbaI TGATCA 1 cut(s) 1137
FblI GTMKAC 1 cut(s) 713
Fnu4HI GCNGC 4 cut(s) 207, 558, 727, 1315
FokI GGATG 2 cut(s) 152, 879
FriOI GRGCYC 1 cut(s) 1185
Fsp4HI GCNGC 4 cut(s) 207, 558, 727, 1315
FspBI CTAG 1 cut(s) 12
GluI GCNGC 4 cut(s) 207, 558, 727, 1315
HaeIII GGCC 5 cut(s) 168, 221, 726, 990, 1365
HapII CCGG 2 cut(s) 1120, 1288
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 6 cut(s) 228, 460, 763, 923, 1018, 1199
HpaII CCGG 2 cut(s) 1120, 1288
HphI GGTGA 5 cut(s) 56, 246, 446, 799, 1172
Hpy166II GTNNAC 3 cut(s) 248, 415, 714
Hpy188I TCNGA 4 cut(s) 354, 465, 504, 1349
Hpy188III TCNNGA 6 cut(s) 105, 156, 745, 807, 1015, 1171
Hpy8I GTNNAC 3 cut(s) 248, 415, 714
HpyAV CCTTC 4 cut(s) 34, 261, 305, 1287
HpyCH4III ACNGT 1 cut(s) 686
HpyCH4IV ACGT 3 cut(s) 160, 679, 1096
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 550, 614, 1362
HpyF3I CTNAG 4 cut(s) 117, 321, 353, 501
HpySE526I ACGT 3 cut(s) 160, 679, 1096
KroI GCCGGC 1 cut(s) 1287
KroNI GCCGGC 1 cut(s) 1289
Ksp22I TGATCA 1 cut(s) 1137
Kzo9I GATC 3 cut(s) 101, 147, 1137
Lsp1109I GCAGC 2 cut(s) 544, 1301
LweI GCATC 1 cut(s) 1153
MabI ACCWGGT 1 cut(s) 256
MaeI CTAG 1 cut(s) 12
MaeII ACGT 3 cut(s) 160, 679, 1096
MaeIII GTNAC 4 cut(s) 173, 675, 680, 787
MalI GATC 3 cut(s) 103, 149, 1139
MboI GATC 3 cut(s) 101, 147, 1137
MboII GAAGA 2 cut(s) 341, 469
MfeI CAATTG 2 cut(s) 56, 1224
MhlI GDGCHC 1 cut(s) 1185
MlsI TGGCCA 1 cut(s) 1365
MluNI TGGCCA 1 cut(s) 1365
MlyI GAGTC 1 cut(s) 237
MnlI CCTC 3 cut(s) 540, 928, 980
Mox20I TGGCCA 1 cut(s) 1365
Mph1103I ATGCAT 2 cut(s) 1168, 1379
MroNI GCCGGC 1 cut(s) 1287
MscI TGGCCA 1 cut(s) 1365
MseI TTAA 5 cut(s) 594, 954, 1100, 1116, 1154
MslI CAYNNNNRTG 2 cut(s) 193, 623
Msp20I TGGCCA 1 cut(s) 1365
MspI CCGG 2 cut(s) 1120, 1288
MspR9I CCNGG 2 cut(s) 258, 897
MunI CAATTG 2 cut(s) 56, 1224
Mva1269I GAATGC 1 cut(s) 1166
MvaI CCWGG 2 cut(s) 258, 897
MwoI GCNNNNNNNGC 4 cut(s) 61, 550, 614, 1362
NaeI GCCGGC 1 cut(s) 1289
NcoI CCATGG 1 cut(s) 194
NdeII GATC 3 cut(s) 101, 147, 1137
NgoMIV GCCGGC 1 cut(s) 1287
NlaIV GGNNCC 3 cut(s) 123, 169, 939
NmeAIII GCCGAG 1 cut(s) 570
NmuCI GTSAC 2 cut(s) 680, 787
NsiI ATGCAT 2 cut(s) 1168, 1379
NspI RCATGY 2 cut(s) 1011, 1045
PaeI GCATGC 1 cut(s) 1045
PciI ACATGT 1 cut(s) 1007
PcsI WCGNNNNNNNCGW 1 cut(s) 685
PctI GAATGC 1 cut(s) 1166
PdiI GCCGGC 1 cut(s) 1289
PfeI GAWTC 5 cut(s) 460, 763, 923, 1018, 1199
PflMI CCANNNNNTGG 1 cut(s) 1068
PkrI GCNGC 4 cut(s) 208, 559, 728, 1316
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
Ppu21I YACGTR 1 cut(s) 680
PscI ACATGT 1 cut(s) 1007
PshBI ATTAAT 1 cut(s) 954
Psp124BI GAGCTC 1 cut(s) 1185
Psp6I CCWGG 2 cut(s) 256, 895
PspGI CCWGG 2 cut(s) 256, 895
PspN4I GGNNCC 3 cut(s) 123, 169, 939
PspPI GGNCC 1 cut(s) 167
PstI CTGCAG 1 cut(s) 658
RsaI GTAC 4 cut(s) 414, 977, 1113, 1255
RsaNI GTAC 4 cut(s) 413, 976, 1112, 1254
RseI CAYNNNNRTG 2 cut(s) 193, 623
SacI GAGCTC 1 cut(s) 1185
SaqAI TTAA 5 cut(s) 594, 954, 1100, 1116, 1154
SatI GCNGC 4 cut(s) 207, 558, 727, 1315
Sau3AI GATC 3 cut(s) 101, 147, 1137
Sau96I GGNCC 1 cut(s) 167
SchI GAGTC 1 cut(s) 237
ScrFI CCNGG 2 cut(s) 258, 897
SduI GDGCHC 1 cut(s) 1185
SexAI ACCWGGT 1 cut(s) 256
SfaNI GCATC 1 cut(s) 1153
SfcI CTRYAG 1 cut(s) 654
SmiMI CAYNNNNRTG 2 cut(s) 193, 623
SphI GCATGC 1 cut(s) 1045
SsiI CCGC 4 cut(s) 206, 421, 667, 727
SspMI CTAG 1 cut(s) 12
SstI GAGCTC 1 cut(s) 1185
StyD4I CCNGG 2 cut(s) 256, 895
StyI CCWWGG 3 cut(s) 194, 754, 997
TaaI ACNGT 1 cut(s) 686
TaiI ACGT 3 cut(s) 163, 682, 1099
TatI WGTACW 2 cut(s) 412, 975
TauI GCSGC 2 cut(s) 209, 729
TfiI GAWTC 5 cut(s) 460, 763, 923, 1018, 1199
Tru1I TTAA 5 cut(s) 594, 954, 1100, 1116, 1154
Tru9I TTAA 5 cut(s) 594, 954, 1100, 1116, 1154
TscAI CASTG 1 cut(s) 736
TseFI GTSAC 2 cut(s) 680, 787
TseI GCWGC 2 cut(s) 557, 1314
Tsp45I GTSAC 2 cut(s) 680, 787
TspDTI ATGAA 6 cut(s) 287, 327, 423, 755, 904, 1212
TspRI CASTG 1 cut(s) 736
Van91I CCANNNNNTGG 1 cut(s) 1068
VspI ATTAAT 1 cut(s) 954
XapI RAATTY 2 cut(s) 359, 1350
XceI RCATGY 2 cut(s) 1011, 1045
XcmI CCANNNNNNNNNTGG 2 cut(s) 158, 752
XmiI GTMKAC 1 cut(s) 713
XspI CTAG 1 cut(s) 12
Zsp2I ATGCAT 2 cut(s) 1168, 1379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.