RchiOBHm_Chr6g0250671

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0016961
Physical Location & Seq
Forward (+)
6063737 .. 6065291
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22470

Sequence Viewer

Length: 1266 bp
ATGACGTCAACTTGGTCTCCCCGTCAACGATGGAAATACGATGTTTTTCTAAGCTTCAGAGGGGAAGATACCCACGAGACTTTCATCTGCCATCTGTATCGTGCACTAACTGCGAGAGGAATTATAACCTTTTTAGATGACGAAAGCCTGGAGAGAGGATCAACCATTGGCCCATCACTTTCTACAGCAATTGAGGACTCAAGATCAGCCCTGGTCGTTTTTTCAGAGAATTTCGCTTCTTCATCTTGGTGCTTGGATGAACTCTCGAAGATTCTTCAATGCAGGGAAGAGAGGGGACAAATAATCTACCCAATCTTCTACCAAGTGGATCCTTCTGATGTGCGCAAGCAAAGGGGAAGTTTTCAGCTCCGTACAGTAGAGGTAAGGGAACATGAAGAAGTTTATGGGAATAAGGACAAACTAAAGGAGTGGACAGATGCTTTGAGAGAGGTGAGCAGGATTGCTGGGTGGGATTCGAGTACATATGATCAGATTATGATCAGATTTCAACTGCAAACTCTTATAGAGGGAGATCGGTGTTTGCATTTATTTGTGCTAAATGAGGATGATGGAAAATTGATTGAGGAAATTGTGATGGATACACTAACAAGTCTACGTGATACTTATACCTCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTGATGATTTGTTGCACAATTGTATTTGTCAACAGTTTGGTGGTGTACGTATTATAGGCATACATGGGATGCGAGGAATAGGCAAGACAACACTTGCTCGAGCTATTTATGATGGACTTGCTCACGGGTTTGGCAGAAGCTGCTTTCTTTTAAATGTTAGAGAAAAATCTGAAAAGGATGGCCTAGTTTCTTTACAAGAAAAACTACTTTCCAGAATCCTGAGGACAAAAGTTGACATTGAGGATGAATATGCAGGAGCTGCTATGATAGAAAGGCGGTTATGTGAACTAAAGGTGCTTGTTGTTATTGATGATGTGGATCACTTCAATCAATTAGATAAATTGGCTGGAAGTCGGGACTGGTTCGGTCCTGGGAGTAGAATTATTGTAACCACCCCAGATATTCATTTGCTAAGGGGACATGATGTTGATGCTACATACAAGGCTACTGGGTTAACTGATGGTGAAGCTATTCAACTATTGAGTTTAAAGGCTTTCAAGAAAAGCTTCCCACCAGAAGATTATTTGGAGTCGTGCCATCATATTTTAGGGTATGCTCAGGGCCGGGGCTTCCCTTGGCTCTTGTGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

421

Amino Acids

48.05

Weight (kDa)

5.47

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 162 9.2e-47 TIR domain
TIR_2 PF13676 15 - 124 4.6e-15 TIR domain
NB-ARC PF00931 238 - 393 3e-16 NB-ARC domain
AAA_22 PF13401 241 - 339 8.1e-06 AAA domain
NACHT PF05729 242 - 339 1e-08 NACHT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 125
AatII GACGTC 1 cut(s) 8
Acc16I TGCGCA 1 cut(s) 344
AccI GTMKAC 1 cut(s) 613
AciI CCGC 2 cut(s) 670, 949
AclWI GGATC 4 cut(s) 166, 323, 336, 999
AcsI RAATTY 1 cut(s) 229
AcuI CTGAAG 1 cut(s) 40
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 373, 481, 720
AgsI TTSAA 6 cut(s) 278, 509, 636, 1000, 1148, 1171
AjnI CCWGG 3 cut(s) 147, 210, 1042
AluBI AGCT 7 cut(s) 54, 367, 776, 813, 932, 1142, 1179
AluI AGCT 7 cut(s) 54, 367, 776, 813, 932, 1142, 1179
Alw21I GWGCWC 1 cut(s) 106
Alw26I GTCTC 2 cut(s) 21, 71
Alw44I GTGCAC 1 cut(s) 102
AlwI GGATC 4 cut(s) 166, 323, 336, 999
AlwNI CAGNNNCTG 2 cut(s) 813, 932
Ama87I CYCGRG 1 cut(s) 771
AoxI GGCC 3 cut(s) 169, 853, 1234
ApaLI GTGCAC 1 cut(s) 102
ApeKI GCWGC 2 cut(s) 813, 932
ApoI RAATTY 1 cut(s) 229
ArsI GACNNNNNNTTYG 2 cut(s) 424, 456
Asp700I GAANNNNTTC 3 cut(s) 664, 1143, 1178
AspLEI GCGC 1 cut(s) 345
AspS9I GGNCC 3 cut(s) 170, 1040, 1234
AsuC2I CCSGG 1 cut(s) 1238
AsuHPI GGTGA 2 cut(s) 463, 1148
AvaI CYCGRG 1 cut(s) 771
AvaII GGWCC 1 cut(s) 1040
AxyI CCTNAGG 1 cut(s) 893
BaeGI GKGCMC 1 cut(s) 106
BamHI GGATCC 1 cut(s) 328
BauI CACGAG 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 106
BbvI GCAGC 2 cut(s) 800, 919
BccI CCATC 9 cut(s) 24, 99, 181, 563, 589, 779, 845, 1127, 1218
BciT130I CCWGG 3 cut(s) 149, 212, 1044
BciVI GTATCC 1 cut(s) 592
BclI TGATCA 2 cut(s) 487, 498
BcnI CCSGG 1 cut(s) 1238
BcoDI GTCTC 2 cut(s) 21, 71
BfaI CTAG 1 cut(s) 857
BfmI CTRYAG 1 cut(s) 183
BfuI GTATCC 1 cut(s) 592
BisI GCNGC 2 cut(s) 814, 933
BlsI GCNGC 2 cut(s) 815, 934
Bme1390I CCNGG 4 cut(s) 149, 212, 1044, 1238
Bme18I GGWCC 1 cut(s) 1040
BmeT110I CYCGRG 1 cut(s) 771
BmgT120I GGNCC 3 cut(s) 170, 1040, 1234
BmiI GGNNCC 1 cut(s) 330
BmrFI CCNGG 4 cut(s) 149, 212, 1044, 1238
BmrI ACTGGG 1 cut(s) 1131
BmsI GCATC 3 cut(s) 427, 732, 1093
BmuI ACTGGG 1 cut(s) 1131
BpmI CTGGAG 1 cut(s) 170
Bpu10I CCTNAGC 2 cut(s) 1085, 1230
BpuEI CTTGAG 1 cut(s) 184
BpuMI CCSGG 1 cut(s) 1238
BsaAI YACGTR 2 cut(s) 617, 722
BsaBI GATNNNNATC 2 cut(s) 497, 990
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 21
BsaJI CCNNGG 4 cut(s) 210, 1043, 1237, 1247
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bse1I ACTGG 2 cut(s) 1037, 1126
Bse21I CCTNAGG 1 cut(s) 893
Bse8I GATNNNNATC 2 cut(s) 497, 990
BseBI CCWGG 3 cut(s) 149, 212, 1044
BseDI CCNNGG 4 cut(s) 210, 1043, 1237, 1247
BseGI GGATG 5 cut(s) 262, 571, 747, 856, 922
BseJI GATNNNNATC 2 cut(s) 497, 990
BseMII CTCAG 2 cut(s) 884, 1244
BseNI ACTGG 2 cut(s) 1037, 1126
BseSI GKGCMC 1 cut(s) 106
BseXI GCAGC 2 cut(s) 800, 919
BseYI CCCAGC 1 cut(s) 464
BshFI GGCC 3 cut(s) 171, 855, 1236
BsiHKAI GWGCWC 1 cut(s) 106
BsiHKCI CYCGRG 1 cut(s) 771
BsiSI CCGG 1 cut(s) 1237
BslFI GGGAC 3 cut(s) 309, 1043, 1104
BsmAI GTCTC 2 cut(s) 21, 71
BsmFI GGGAC 3 cut(s) 309, 1043, 1104
BsnI GGCC 3 cut(s) 171, 855, 1236
Bso31I GGTCTC 1 cut(s) 21
BsoBI CYCGRG 1 cut(s) 771
Bsp1286I GDGCHC 1 cut(s) 106
Bsp143I GATC 7 cut(s) 158, 203, 328, 487, 498, 532, 991
BspACI CCGC 2 cut(s) 670, 949
BspANI GGCC 3 cut(s) 171, 855, 1236
BspCNI CTCAG 2 cut(s) 885, 1243
BspLI GGNNCC 1 cut(s) 330
BspPI GGATC 4 cut(s) 166, 323, 336, 999
BspTNI GGTCTC 1 cut(s) 21
BsrI ACTGG 2 cut(s) 1037, 1126
BssECI CCNNGG 4 cut(s) 210, 1043, 1237, 1247
BssMI GATC 7 cut(s) 158, 203, 328, 487, 498, 532, 991
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 74
BssT1I CCWWGG 1 cut(s) 1247
Bst2BI CACGAG 1 cut(s) 74
Bst2UI CCWGG 3 cut(s) 149, 212, 1044
Bst4CI ACNGT 2 cut(s) 376, 708
Bst6I CTCTTC 2 cut(s) 282, 637
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 3 cut(s) 110, 813, 932
BstBAI YACGTR 2 cut(s) 617, 722
BstC8I GCNNGC 1 cut(s) 347
BstDEI CTNAG 4 cut(s) 50, 893, 1085, 1230
BstF5I GGATG 5 cut(s) 262, 571, 747, 856, 922
BstHHI GCGC 1 cut(s) 345
BstKTI GATC 7 cut(s) 161, 206, 331, 490, 501, 535, 994
BstMAI GTCTC 2 cut(s) 21, 71
BstMBI GATC 7 cut(s) 158, 203, 328, 487, 498, 532, 991
BstMWI GCNNNNNNNGC 3 cut(s) 110, 813, 932
BstNI CCWGG 3 cut(s) 149, 212, 1044
BstSCI CCNGG 4 cut(s) 147, 210, 1042, 1236
BstSFI CTRYAG 1 cut(s) 183
BstSLI GKGCMC 1 cut(s) 106
BstSNI TACGTA 1 cut(s) 722
BstV1I GCAGC 2 cut(s) 800, 919
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
Bsu36I CCTNAGG 1 cut(s) 893
BsuI GTATCC 1 cut(s) 592
BsuRI GGCC 3 cut(s) 171, 855, 1236
BtsCI GGATG 5 cut(s) 262, 571, 747, 856, 922
Cac8I GCNNGC 1 cut(s) 347
CaiI CAGNNNCTG 2 cut(s) 813, 932
CfoI GCGC 1 cut(s) 345
Cfr13I GGNCC 3 cut(s) 170, 1040, 1234
Csp6I GTAC 3 cut(s) 372, 480, 719
CviAII CATG 3 cut(s) 392, 737, 1094
CviQI GTAC 3 cut(s) 372, 480, 719
DdeI CTNAG 4 cut(s) 50, 893, 1085, 1230
DpnI GATC 7 cut(s) 160, 205, 330, 489, 500, 534, 993
DpnII GATC 7 cut(s) 158, 203, 328, 487, 498, 532, 991
DraI TTTAAA 2 cut(s) 825, 1161
Eam1104I CTCTTC 2 cut(s) 282, 637
EarI CTCTTC 2 cut(s) 282, 637
Eco105I TACGTA 1 cut(s) 722
Eco130I CCWWGG 1 cut(s) 1247
Eco31I GGTCTC 1 cut(s) 21
Eco47I GGWCC 1 cut(s) 1040
Eco57I CTGAAG 1 cut(s) 40
Eco81I CCTNAGG 1 cut(s) 893
Eco88I CYCGRG 1 cut(s) 771
EcoRII CCWGG 3 cut(s) 147, 210, 1042
EcoT14I CCWWGG 1 cut(s) 1247
ErhI CCWWGG 1 cut(s) 1247
FaeI CATG 3 cut(s) 395, 740, 1097
FalI AAGNNNNNCTT 2 cut(s) 1163, 1195
FaqI GGGAC 3 cut(s) 309, 1043, 1104
FatI CATG 3 cut(s) 391, 736, 1093
FauI CCCGC 1 cut(s) 677
FauNDI CATATG 1 cut(s) 484
FbaI TGATCA 2 cut(s) 487, 498
FblI GTMKAC 1 cut(s) 613
Fnu4HI GCNGC 2 cut(s) 814, 933
FokI GGATG 5 cut(s) 269, 578, 754, 863, 929
Fsp4HI GCNGC 2 cut(s) 814, 933
FspBI CTAG 1 cut(s) 857
FspI TGCGCA 1 cut(s) 344
GlaI GCGC 1 cut(s) 344
GluI GCNGC 2 cut(s) 814, 933
GsaI CCCAGC 1 cut(s) 468
GsuI CTGGAG 1 cut(s) 170
HaeIII GGCC 3 cut(s) 171, 855, 1236
HapII CCGG 1 cut(s) 1237
HhaI GCGC 1 cut(s) 345
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 395, 740, 1097
Hin6I GCGC 1 cut(s) 343
HinP1I GCGC 1 cut(s) 343
HincII GTYRAC 6 cut(s) 9, 26, 643, 704, 907, 1128
HindII GTYRAC 6 cut(s) 9, 26, 643, 704, 907, 1128
HindIII AAGCTT 2 cut(s) 52, 1177
HinfI GANTC 6 cut(s) 197, 271, 473, 665, 888, 1202
HpaI GTTAAC 1 cut(s) 1128
HpaII CCGG 1 cut(s) 1237
HphI GGTGA 2 cut(s) 463, 1148
Hpy188I TCNGA 6 cut(s) 59, 226, 337, 492, 503, 844
Hpy188III TCNNGA 6 cut(s) 201, 265, 885, 892, 1028, 1171
HpyAV CCTTC 1 cut(s) 342
HpyCH4III ACNGT 2 cut(s) 376, 708
HpyCH4IV ACGT 3 cut(s) 5, 616, 721
HpyCH4V TGCA 6 cut(s) 104, 282, 514, 544, 688, 926
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 813, 932
HpyF3I CTNAG 4 cut(s) 50, 893, 1085, 1230
HpySE526I ACGT 3 cut(s) 5, 616, 721
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 395, 740, 1097
HspAI GCGC 1 cut(s) 343
Ksp22I TGATCA 2 cut(s) 487, 498
KspAI GTTAAC 1 cut(s) 1128
Kzo9I GATC 7 cut(s) 158, 203, 328, 487, 498, 532, 991
LmnI GCTCC 2 cut(s) 372, 929
Lsp1109I GCAGC 2 cut(s) 800, 919
LweI GCATC 3 cut(s) 427, 732, 1093
MaeI CTAG 1 cut(s) 857
MaeII ACGT 3 cut(s) 5, 616, 721
MaeIII GTNAC 1 cut(s) 1060
MalI GATC 7 cut(s) 160, 205, 330, 489, 500, 534, 993
MboI GATC 7 cut(s) 158, 203, 328, 487, 498, 532, 991
MboII GAAGA 9 cut(s) 77, 231, 266, 280, 299, 307, 407, 624, 1202
MfeI CAATTG 2 cut(s) 189, 691
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 1 cut(s) 106
MluCI AATT 9 cut(s) 120, 189, 229, 575, 588, 691, 1004, 1013, 1053
MlyI GAGTC 2 cut(s) 191, 1211
MroXI GAANNNNTTC 3 cut(s) 664, 1143, 1178
MseI TTAA 3 cut(s) 824, 1127, 1160
MslI CAYNNNNRTG 2 cut(s) 247, 659
MspI CCGG 1 cut(s) 1237
MspR9I CCNGG 4 cut(s) 149, 212, 1044, 1238
MunI CAATTG 2 cut(s) 189, 691
MvaI CCWGG 3 cut(s) 149, 212, 1044
MwoI GCNNNNNNNGC 3 cut(s) 110, 813, 932
NciI CCSGG 1 cut(s) 1238
NdeI CATATG 1 cut(s) 484
NdeII GATC 7 cut(s) 158, 203, 328, 487, 498, 532, 991
NlaIII CATG 3 cut(s) 395, 740, 1097
NlaIV GGNNCC 1 cut(s) 330
NsbI TGCGCA 1 cut(s) 344
PaeR7I CTCGAG 1 cut(s) 771
PdmI GAANNNNTTC 3 cut(s) 664, 1143, 1178
PfeI GAWTC 4 cut(s) 271, 473, 665, 888
PkrI GCNGC 2 cut(s) 815, 934
PleI GAGTC 2 cut(s) 191, 1210
PpsI GAGTC 2 cut(s) 191, 1210
Ppu21I YACGTR 2 cut(s) 617, 722
PsiI TTATAA 1 cut(s) 125
Psp6I CCWGG 3 cut(s) 147, 210, 1042
PspFI CCCAGC 1 cut(s) 464
PspGI CCWGG 3 cut(s) 147, 210, 1042
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 3 cut(s) 170, 1040, 1234
PspXI VCTCGAGB 1 cut(s) 771
PstNI CAGNNNCTG 2 cut(s) 813, 932
PsuI RGATCY 1 cut(s) 328
RsaI GTAC 3 cut(s) 373, 481, 720
RsaNI GTAC 3 cut(s) 372, 480, 719
RseI CAYNNNNRTG 2 cut(s) 247, 659
SaqAI TTAA 3 cut(s) 824, 1127, 1160
SatI GCNGC 2 cut(s) 814, 933
Sau3AI GATC 7 cut(s) 158, 203, 328, 487, 498, 532, 991
Sau96I GGNCC 3 cut(s) 170, 1040, 1234
SchI GAGTC 2 cut(s) 191, 1211
ScrFI CCNGG 4 cut(s) 149, 212, 1044, 1238
SduI GDGCHC 1 cut(s) 106
SfaNI GCATC 3 cut(s) 427, 732, 1093
SfcI CTRYAG 1 cut(s) 183
Sfr274I CTCGAG 1 cut(s) 771
SinI GGWCC 1 cut(s) 1040
SlaI CTCGAG 1 cut(s) 771
SmiMI CAYNNNNRTG 2 cut(s) 247, 659
SmlI CTYRAG 2 cut(s) 199, 771
SmoI CTYRAG 2 cut(s) 199, 771
SnaBI TACGTA 1 cut(s) 722
Sse9I AATT 9 cut(s) 120, 189, 229, 575, 588, 691, 1004, 1013, 1053
SsiI CCGC 2 cut(s) 670, 949
SspMI CTAG 1 cut(s) 857
StyD4I CCNGG 4 cut(s) 147, 210, 1042, 1236
StyI CCWWGG 1 cut(s) 1247
TaaI ACNGT 2 cut(s) 376, 708
TaiI ACGT 3 cut(s) 8, 619, 724
TaqI TCGA 3 cut(s) 266, 476, 772
TaqII GACCGA 1 cut(s) 1028
TasI AATT 9 cut(s) 120, 189, 229, 575, 588, 691, 1004, 1013, 1053
TatI WGTACW 1 cut(s) 479
TfiI GAWTC 4 cut(s) 271, 473, 665, 888
Tru1I TTAA 3 cut(s) 824, 1127, 1160
Tru9I TTAA 3 cut(s) 824, 1127, 1160
TseI GCWGC 2 cut(s) 813, 932
TspDTI ATGAA 7 cut(s) 73, 231, 273, 408, 643, 933, 1067
TspGWI ACGGA 1 cut(s) 359
VneI GTGCAC 1 cut(s) 102
VpaK11BI GGWCC 1 cut(s) 1040
XapI RAATTY 1 cut(s) 229
XhoI CTCGAG 1 cut(s) 771
XmiI GTMKAC 1 cut(s) 613
XmnI GAANNNNTTC 3 cut(s) 664, 1143, 1178
XspI CTAG 1 cut(s) 857
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.