RLG00000030321

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0016961
Physical Location & Seq
Reverse (-)
59593615 .. 59594121
507 bp
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UTR
Exon/CDS
Intron
RLM00000030321

Sequence Viewer

Length: 399 bp
ATGGCAAGTGCAATAGATAGGCTAAAGAAAACACCACATGAACGCGTTATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAGAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTTGACTATTGCCAGCTAAACCCTGTCATTGGTTTAAGTGTTCTTGCTGATCGATGTCTCATAACTATCTCCAACAACGAACTGTCGATGCATGATTTGCTACAAGAAATGGGCCGGGAAATTGTTCGTGAACAGTCTCCTAAACAGCCAGGAAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTCATGAAGAGACATAAGGGAAGAAAAATTCAAACCAAGGCATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

133

Amino Acids

15.45

Weight (kDa)

9.24

Isoelectric Point (pI)

58.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 28 - 99 1.7e-24 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 393
AccII CGCG 1 cut(s) 45
AclWI GGATC 1 cut(s) 150
AcsI RAATTY 1 cut(s) 378
AfiI CCNNNNNNNGG 2 cut(s) 188, 393
AflIII ACRYGT 1 cut(s) 43
AgsI TTSAA 1 cut(s) 383
AjnI CCWGG 1 cut(s) 307
AluBI AGCT 1 cut(s) 175
AluI AGCT 1 cut(s) 175
Alw26I GTCTC 4 cut(s) 221, 300, 336, 355
AlwI GGATC 1 cut(s) 150
AoxI GGCC 1 cut(s) 271
ApoI RAATTY 1 cut(s) 378
Asp700I GAANNNNTTC 1 cut(s) 282
AspS9I GGNCC 1 cut(s) 271
AsuC2I CCSGG 1 cut(s) 275
BbsI GAAGAC 1 cut(s) 345
BccI CCATC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 309
BcnI CCSGG 1 cut(s) 275
BcoDI GTCTC 4 cut(s) 221, 300, 336, 355
Bme1390I CCNGG 2 cut(s) 275, 309
BmgT120I GGNCC 1 cut(s) 271
BmrFI CCNGG 2 cut(s) 275, 309
BmsI GCATC 1 cut(s) 237
BpiI GAAGAC 1 cut(s) 345
BpuMI CCSGG 1 cut(s) 275
Bsa29I ATCGAT 1 cut(s) 211
BsaBI GATNNNNATC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 336
BsaJI CCNNGG 1 cut(s) 387
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 393
Bse1I ACTGG 1 cut(s) 84
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 1 cut(s) 309
BseCI ATCGAT 1 cut(s) 211
BseDI CCNNGG 1 cut(s) 387
BseGI GGATG 1 cut(s) 88
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 2 cut(s) 188, 393
BseNI ACTGG 1 cut(s) 84
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 1 cut(s) 273
BshVI ATCGAT 1 cut(s) 211
BsiSI CCGG 1 cut(s) 274
BslI CCNNNNNNNGG 2 cut(s) 188, 393
BsmAI GTCTC 4 cut(s) 221, 300, 336, 355
BsnI GGCC 1 cut(s) 273
Bso31I GGTCTC 1 cut(s) 336
Bsp143I GATC 2 cut(s) 142, 208
BspANI GGCC 1 cut(s) 273
BspDI ATCGAT 1 cut(s) 211
BspFNI CGCG 1 cut(s) 45
BspHI TCATGA 2 cut(s) 334, 354
BspPI GGATC 1 cut(s) 150
BspTNI GGTCTC 1 cut(s) 336
BsrI ACTGG 1 cut(s) 84
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 2 cut(s) 142, 208
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 2 cut(s) 243, 294
Bst6I CTCTTC 1 cut(s) 353
BstAPI GCANNNNNTGC 1 cut(s) 256
BstC8I GCNNGC 2 cut(s) 118, 173
BstF5I GGATG 1 cut(s) 88
BstFNI CGCG 1 cut(s) 45
BstKTI GATC 2 cut(s) 145, 211
BstMAI GTCTC 4 cut(s) 221, 300, 336, 355
BstMBI GATC 2 cut(s) 142, 208
BstMWI GCNNNNNNNGC 1 cut(s) 256
BstNI CCWGG 1 cut(s) 309
BstSCI CCNGG 2 cut(s) 273, 307
BstUI CGCG 1 cut(s) 45
BstV2I GAAGAC 1 cut(s) 345
Bsu15I ATCGAT 1 cut(s) 211
BsuRI GGCC 1 cut(s) 273
BsuTUI ATCGAT 1 cut(s) 211
BtsCI GGATG 1 cut(s) 88
Cac8I GCNNGC 2 cut(s) 118, 173
CciI TCATGA 2 cut(s) 334, 354
Cfr13I GGNCC 1 cut(s) 271
ClaI ATCGAT 1 cut(s) 211
CviAII CATG 5 cut(s) 38, 251, 335, 355, 393
CviJI RGCY 4 cut(s) 22, 175, 273, 307
CviKI_1 RGCY 4 cut(s) 22, 175, 273, 307
DpnI GATC 2 cut(s) 144, 210
DpnII GATC 2 cut(s) 142, 208
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
Eco130I CCWWGG 1 cut(s) 387
Eco31I GGTCTC 1 cut(s) 336
EcoRII CCWGG 1 cut(s) 307
EcoT14I CCWWGG 1 cut(s) 387
EcoT22I ATGCAT 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 5 cut(s) 41, 254, 338, 358, 396
FaiI YATR 9 cut(s) 39, 111, 221, 252, 328, 336, 356, 366, 394
FatI CATG 5 cut(s) 37, 250, 334, 354, 392
FokI GGATG 1 cut(s) 95
HaeIII GGCC 1 cut(s) 273
HapII CCGG 1 cut(s) 274
Hin1II CATG 5 cut(s) 41, 254, 338, 358, 396
HpaII CCGG 1 cut(s) 274
Hpy166II GTNNAC 1 cut(s) 290
Hpy188I TCNGA 1 cut(s) 63
Hpy188III TCNNGA 3 cut(s) 287, 335, 355
Hpy8I GTNNAC 1 cut(s) 290
HpyAV CCTTC 1 cut(s) 127
HpyCH4III ACNGT 2 cut(s) 243, 294
HpyCH4IV ACGT 1 cut(s) 316
HpyCH4V TGCA 2 cut(s) 11, 250
HpyF10VI GCNNNNNNNGC 1 cut(s) 256
HpySE526I ACGT 1 cut(s) 316
Hsp92II CATG 5 cut(s) 41, 254, 338, 358, 396
Kzo9I GATC 2 cut(s) 142, 208
LpnPI CCDG 6 cut(s) 65, 185, 195, 287, 294, 321
LweI GCATC 1 cut(s) 237
MaeII ACGT 1 cut(s) 316
MaeIII GTNAC 1 cut(s) 148
MalI GATC 2 cut(s) 144, 210
MboI GATC 2 cut(s) 142, 208
MboII GAAGA 3 cut(s) 350, 370, 384
MluCI AATT 2 cut(s) 279, 378
MluI ACGCGT 1 cut(s) 43
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 1 cut(s) 46
Mph1103I ATGCAT 1 cut(s) 252
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 1 cut(s) 194
MspI CCGG 1 cut(s) 274
MspR9I CCNGG 2 cut(s) 275, 309
MvaI CCWGG 1 cut(s) 309
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 1 cut(s) 256
NciI CCSGG 1 cut(s) 275
NdeII GATC 2 cut(s) 142, 208
NlaIII CATG 5 cut(s) 41, 254, 338, 358, 396
NmuCI GTSAC 1 cut(s) 148
NsiI ATGCAT 1 cut(s) 252
PagI TCATGA 2 cut(s) 334, 354
PdmI GAANNNNTTC 1 cut(s) 282
PflMI CCANNNNNTGG 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 307
PspGI CCWGG 1 cut(s) 307
PspPI GGNCC 1 cut(s) 271
SaqAI TTAA 1 cut(s) 194
Sau3AI GATC 2 cut(s) 142, 208
Sau96I GGNCC 1 cut(s) 271
ScrFI CCNGG 2 cut(s) 275, 309
SetI ASST 3 cut(s) 57, 177, 319
SfaNI GCATC 1 cut(s) 237
Sse9I AATT 2 cut(s) 279, 378
SspI AATATT 1 cut(s) 102
StyD4I CCNGG 2 cut(s) 273, 307
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 2 cut(s) 243, 294
TaiI ACGT 1 cut(s) 319
TaqI TCGA 2 cut(s) 211, 245
TasI AATT 2 cut(s) 279, 378
Tru1I TTAA 1 cut(s) 194
Tru9I TTAA 1 cut(s) 194
TseFI GTSAC 1 cut(s) 148
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 4 cut(s) 54, 99, 351, 371
Van91I CCANNNNNTGG 1 cut(s) 393
XapI RAATTY 1 cut(s) 378
XmnI GAANNNNTTC 1 cut(s) 282
Zsp2I ATGCAT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.