RLG00000015492

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016961
Physical Location & Seq
Reverse (-)
66700001 .. 66707648
7648 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015492

Sequence Viewer

Length: 726 bp
ATGGATGTGCTTCAGATTAGTTTTGATGGACTAGAGGAAAAAGATCAACAGATATTCCTACATATTGCTTGTTTGTACAAGGGGAAGGACAGGGATCGTGTGACACAAATACTAGACTATTGCAAGCTAGAACCTGGCATTGGTTTAAAGGTTCTTGAGGAAAAATCTCTCATTACTATTTTTAACAACAAACTGTCGATGCATGATTTGCTACAAGAAATGGGCTTGGAAATAGTTCGTCGAGAGTCCCCTGATGAGCCAGACTGGAGGGGAAATAACACCATTAGCCTTGGCCTTCTTGATAGAATAAAGGGATGTCTTGGCGTCTCTGAGCTTCTCGGAGCGACCTTGGTTTCGAAACCTAGGTTTCGATTAGCGGCGGTGTTTCTTCCTTCGGGTAAGGCGGCGATCGTGGACTGCTGCGACGTAGGGAGGGATCTCTGCGGTGACGTAGGGAGGGATCTCTGCGGCGAGGGATTTTTTCTCAGACCGTGGCAGAGGGTTTTGGTCCAGGTGGTGAGTTCGCTTTTATCGTCTGGGCTGCAGAGGAGTGGGCGAGTCGGATCCAGCGGTGGCGGTTTGGTCTTGAGGTCACAAGGATTTGAGTCGGGTTTAGCGCCGAAGATCATCGCTTGGGCTATAGTTGTGGTCTACTGGTGCAGCGGTGGTTTGGAGGCGGTTTTGCGGCTGTTTGCGGTTGCTCAAAGGCCGGTTTCATCTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

26.42

Weight (kDa)

7.52

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 12 - 83 2.3e-20 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 651
AclWI GGATC 5 cut(s) 102, 444, 468, 558, 571
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 1 cut(s) 77
AfiI CCNNNNNNNGG 1 cut(s) 140
AjnI CCWGG 2 cut(s) 133, 510
AjuI GAANNNNNNNTTGG 2 cut(s) 123, 155
AluBI AGCT 2 cut(s) 127, 334
AluI AGCT 2 cut(s) 127, 334
Alw26I GTCTC 1 cut(s) 331
AlwI GGATC 5 cut(s) 102, 444, 468, 558, 571
AoxI GGCC 2 cut(s) 292, 707
ApeKI GCWGC 3 cut(s) 420, 541, 660
ArsI GACNNNNNNTTYG 2 cut(s) 337, 369
Asp700I GAANNNNTTC 1 cut(s) 234
AspA2I CCTAGG 1 cut(s) 362
AspLEI GCGC 1 cut(s) 619
AspS9I GGNCC 1 cut(s) 508
AsuHPI GGTGA 2 cut(s) 458, 529
AsuII TTCGAA 1 cut(s) 356
AvaII GGWCC 1 cut(s) 508
AvrII CCTAGG 1 cut(s) 362
BamHI GGATCC 1 cut(s) 563
BbvI GCAGC 3 cut(s) 407, 528, 672
BccI CCATC 1 cut(s) 20
BciT130I CCWGG 2 cut(s) 135, 512
BcoDI GTCTC 1 cut(s) 331
BfaI CTAG 5 cut(s) 32, 113, 128, 363, 720
BfmI CTRYAG 2 cut(s) 542, 639
BfoI RGCGCY 1 cut(s) 620
BisI GCNGC 7 cut(s) 378, 405, 421, 469, 542, 661, 686
BlnI CCTAGG 1 cut(s) 362
BlsI GCNGC 7 cut(s) 379, 406, 422, 470, 543, 662, 687
Bme1390I CCNGG 2 cut(s) 135, 512
Bme18I GGWCC 1 cut(s) 508
BmgT120I GGNCC 1 cut(s) 508
BmiI GGNNCC 1 cut(s) 565
BmrFI CCNGG 2 cut(s) 135, 512
BmsI GCATC 1 cut(s) 189
BpmI CTGGAG 1 cut(s) 286
Bpu14I TTCGAA 1 cut(s) 356
BpuEI CTTGAG 2 cut(s) 176, 607
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 4 cut(s) 289, 348, 362, 491
Bsc4I CCNNNNNNNGG 1 cut(s) 140
Bse118I RCCGGY 1 cut(s) 709
Bse1I ACTGG 2 cut(s) 269, 659
BseBI CCWGG 2 cut(s) 135, 512
BseDI CCNNGG 4 cut(s) 289, 348, 362, 491
BseGI GGATG 2 cut(s) 10, 320
BseLI CCNNNNNNNGG 1 cut(s) 140
BseMII CTCAG 2 cut(s) 321, 499
BseNI ACTGG 2 cut(s) 269, 659
BseRI GAGGAG 1 cut(s) 562
BseXI GCAGC 3 cut(s) 407, 528, 672
BsgI GTGCAG 1 cut(s) 679
Bsh1285I CGRYCG 1 cut(s) 411
BshFI GGCC 2 cut(s) 294, 709
BsiEI CGRYCG 1 cut(s) 411
BsiSI CCGG 1 cut(s) 710
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 1 cut(s) 140
BsmAI GTCTC 1 cut(s) 331
BsmBI CGTCTC 1 cut(s) 331
BsmFI GGGAC 1 cut(s) 232
BsnI GGCC 2 cut(s) 294, 709
Bsp119I TTCGAA 1 cut(s) 356
Bsp1407I TGTACA 1 cut(s) 75
Bsp143I GATC 7 cut(s) 43, 94, 408, 436, 460, 563, 624
BspANI GGCC 2 cut(s) 294, 709
BspCNI CTCAG 2 cut(s) 322, 498
BspLI GGNNCC 1 cut(s) 565
BspMAI CTGCAG 1 cut(s) 546
BspPI GGATC 5 cut(s) 102, 444, 468, 558, 571
BspT104I TTCGAA 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 709
BsrGI TGTACA 1 cut(s) 75
BsrI ACTGG 2 cut(s) 269, 659
BssAI RCCGGY 1 cut(s) 709
BssECI CCNNGG 4 cut(s) 289, 348, 362, 491
BssMI GATC 7 cut(s) 43, 94, 408, 436, 460, 563, 624
BssNI GRCGYC 1 cut(s) 324
BssT1I CCWWGG 3 cut(s) 289, 348, 362
Bst2UI CCWGG 2 cut(s) 135, 512
Bst4CI ACNGT 2 cut(s) 195, 492
BstACI GRCGYC 1 cut(s) 324
BstAPI GCANNNNNTGC 1 cut(s) 208
BstAUI TGTACA 1 cut(s) 75
BstBI TTCGAA 1 cut(s) 356
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 2 cut(s) 330, 485
BstDSI CCRYGG 1 cut(s) 491
BstF5I GGATG 2 cut(s) 10, 320
BstH2I RGCGCY 1 cut(s) 620
BstHHI GCGC 1 cut(s) 619
BstKTI GATC 7 cut(s) 46, 97, 411, 439, 463, 566, 627
BstMAI GTCTC 1 cut(s) 331
BstMBI GATC 7 cut(s) 43, 94, 408, 436, 460, 563, 624
BstMCI CGRYCG 1 cut(s) 411
BstMWI GCNNNNNNNGC 1 cut(s) 208
BstNI CCWGG 2 cut(s) 135, 512
BstSCI CCNGG 2 cut(s) 133, 510
BstSFI CTRYAG 2 cut(s) 542, 639
BstV1I GCAGC 3 cut(s) 407, 528, 672
BstX2I RGATCY 3 cut(s) 436, 460, 563
BstYI RGATCY 3 cut(s) 436, 460, 563
BsuRI GGCC 2 cut(s) 294, 709
BtgI CCRYGG 1 cut(s) 491
BtgZI GCGATG 1 cut(s) 613
BtsCI GGATG 2 cut(s) 10, 320
Cac8I GCNNGC 1 cut(s) 125
CfoI GCGC 1 cut(s) 619
Cfr10I RCCGGY 1 cut(s) 709
Cfr13I GGNCC 1 cut(s) 508
CseI GACGC 1 cut(s) 313
Csp6I GTAC 1 cut(s) 76
CviAII CATG 1 cut(s) 203
CviQI GTAC 1 cut(s) 76
DdeI CTNAG 2 cut(s) 330, 485
DpnI GATC 7 cut(s) 45, 96, 410, 438, 462, 565, 626
DpnII GATC 7 cut(s) 43, 94, 408, 436, 460, 563, 624
DraI TTTAAA 1 cut(s) 147
Eco130I CCWWGG 3 cut(s) 289, 348, 362
Eco47I GGWCC 1 cut(s) 508
EcoRII CCWGG 2 cut(s) 133, 510
EcoT14I CCWWGG 3 cut(s) 289, 348, 362
EcoT22I ATGCAT 1 cut(s) 204
ErhI CCWWGG 3 cut(s) 289, 348, 362
Esp3I CGTCTC 1 cut(s) 331
FaeI CATG 1 cut(s) 206
FaiI YATR 3 cut(s) 63, 204, 641
FaqI GGGAC 1 cut(s) 232
FatI CATG 1 cut(s) 202
FblI GTMKAC 1 cut(s) 651
Fnu4HI GCNGC 7 cut(s) 378, 405, 421, 469, 542, 661, 686
FokI GGATG 2 cut(s) 17, 327
Fsp4HI GCNGC 7 cut(s) 378, 405, 421, 469, 542, 661, 686
FspBI CTAG 5 cut(s) 32, 113, 128, 363, 720
GlaI GCGC 1 cut(s) 618
GluI GCNGC 7 cut(s) 378, 405, 421, 469, 542, 661, 686
GsuI CTGGAG 1 cut(s) 286
HaeII RGCGCY 1 cut(s) 620
HaeIII GGCC 2 cut(s) 294, 709
HapII CCGG 1 cut(s) 710
HgaI GACGC 1 cut(s) 313
HhaI GCGC 1 cut(s) 619
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 1 cut(s) 206
Hin6I GCGC 1 cut(s) 617
HinP1I GCGC 1 cut(s) 617
HinfI GANTC 3 cut(s) 245, 558, 605
HpaII CCGG 1 cut(s) 710
HphI GGTGA 2 cut(s) 458, 529
Hpy166II GTNNAC 2 cut(s) 415, 652
Hpy188I TCNGA 5 cut(s) 15, 331, 341, 488, 563
Hpy188III TCNNGA 4 cut(s) 155, 242, 299, 586
Hpy8I GTNNAC 2 cut(s) 415, 652
Hpy99I CGWCG 2 cut(s) 243, 428
HpyAV CCTTC 3 cut(s) 79, 305, 402
HpyCH4III ACNGT 2 cut(s) 195, 492
HpyCH4IV ACGT 2 cut(s) 426, 450
HpyCH4V TGCA 4 cut(s) 123, 202, 544, 660
HpyF10VI GCNNNNNNNGC 1 cut(s) 208
HpyF3I CTNAG 2 cut(s) 330, 485
HpySE526I ACGT 2 cut(s) 426, 450
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 1 cut(s) 206
HspAI GCGC 1 cut(s) 617
Kzo9I GATC 7 cut(s) 43, 94, 408, 436, 460, 563, 624
LmnI GCTCC 1 cut(s) 341
Lsp1109I GCAGC 3 cut(s) 407, 528, 672
LweI GCATC 1 cut(s) 189
MaeI CTAG 5 cut(s) 32, 113, 128, 363, 720
MaeII ACGT 2 cut(s) 426, 450
MaeIII GTNAC 3 cut(s) 100, 446, 591
MalI GATC 7 cut(s) 45, 96, 410, 438, 462, 565, 626
MboI GATC 7 cut(s) 43, 94, 408, 436, 460, 563, 624
MboII GAAGA 2 cut(s) 380, 634
MflI RGATCY 3 cut(s) 436, 460, 563
MlyI GAGTC 3 cut(s) 254, 567, 614
MmeI TCCRAC 1 cut(s) 541
Mph1103I ATGCAT 1 cut(s) 204
MroXI GAANNNNTTC 1 cut(s) 234
MseI TTAA 2 cut(s) 146, 183
MslI CAYNNNNRTG 1 cut(s) 721
MspA1I CMGCKG 2 cut(s) 570, 663
MspI CCGG 1 cut(s) 710
MspR9I CCNGG 2 cut(s) 135, 512
MvaI CCWGG 2 cut(s) 135, 512
MwoI GCNNNNNNNGC 1 cut(s) 208
NdeII GATC 7 cut(s) 43, 94, 408, 436, 460, 563, 624
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 1 cut(s) 565
NmuCI GTSAC 3 cut(s) 100, 446, 591
NsiI ATGCAT 1 cut(s) 204
NspV TTCGAA 1 cut(s) 356
PcsI WCGNNNNNNNCGW 1 cut(s) 530
PdmI GAANNNNTTC 1 cut(s) 234
PkrI GCNGC 7 cut(s) 379, 406, 422, 470, 543, 662, 687
Ple19I CGATCG 1 cut(s) 411
PleI GAGTC 3 cut(s) 253, 566, 613
PpsI GAGTC 3 cut(s) 253, 566, 613
Psp6I CCWGG 2 cut(s) 133, 510
PspGI CCWGG 2 cut(s) 133, 510
PspN4I GGNNCC 1 cut(s) 565
PspPI GGNCC 1 cut(s) 508
PstI CTGCAG 1 cut(s) 546
PsuI RGATCY 3 cut(s) 436, 460, 563
PvuI CGATCG 1 cut(s) 411
RsaI GTAC 1 cut(s) 77
RsaNI GTAC 1 cut(s) 76
RseI CAYNNNNRTG 1 cut(s) 721
SaqAI TTAA 2 cut(s) 146, 183
SatI GCNGC 7 cut(s) 378, 405, 421, 469, 542, 661, 686
Sau3AI GATC 7 cut(s) 43, 94, 408, 436, 460, 563, 624
Sau96I GGNCC 1 cut(s) 508
SchI GAGTC 3 cut(s) 254, 567, 614
ScrFI CCNGG 2 cut(s) 135, 512
SfaNI GCATC 1 cut(s) 189
SfcI CTRYAG 2 cut(s) 542, 639
SfuI TTCGAA 1 cut(s) 356
SinI GGWCC 1 cut(s) 508
SmiMI CAYNNNNRTG 1 cut(s) 721
SmlI CTYRAG 2 cut(s) 155, 586
SmoI CTYRAG 2 cut(s) 155, 586
SspMI CTAG 5 cut(s) 32, 113, 128, 363, 720
StyD4I CCNGG 2 cut(s) 133, 510
StyI CCWWGG 3 cut(s) 289, 348, 362
TaaI ACNGT 2 cut(s) 195, 492
TaiI ACGT 2 cut(s) 429, 453
TaqI TCGA 4 cut(s) 197, 241, 356, 370
TatI WGTACW 1 cut(s) 75
TauI GCSGC 4 cut(s) 380, 407, 471, 688
Tru1I TTAA 2 cut(s) 146, 183
Tru9I TTAA 2 cut(s) 146, 183
TseFI GTSAC 3 cut(s) 100, 446, 591
TseI GCWGC 3 cut(s) 420, 541, 660
Tsp45I GTSAC 3 cut(s) 100, 446, 591
TspDTI ATGAA 1 cut(s) 705
VpaK11BI GGWCC 1 cut(s) 508
XmaJI CCTAGG 1 cut(s) 362
XmiI GTMKAC 1 cut(s) 651
XmnI GAANNNNTTC 1 cut(s) 234
XspI CTAG 5 cut(s) 32, 113, 128, 363, 720
Zsp2I ATGCAT 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.