RchiOBHm_Chr6g0268381

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0016961
Physical Location & Seq
Forward (+)
25264361 .. 25265275
915 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24074

Sequence Viewer

Length: 378 bp
ATGTTAGATGGTTTTAAAAAGAATCCTCCTAGGCACATTCAAGATATTCTCAAAGTCAGTTATGATGGACTGGGAAAAATAGAGAAAGAAGTTTTCCTCGACATTGCTTGTTTCTTCAAAGGTTGGAATAGAAATGATGTGATACAAATACTTGAAGGTTGTGACCGCATTAACCCCGAGCATAGTATTAAAGTTCTCAAAGAAAAGGCGCTCATATATGTTGATGTGTATGGTCAAATTTGCATGCATGATTTGTTGGAAGAGATGGGAAAAGATAAAGTTTTGCAAGAGTCTACAGAGCCCGGTAAGCGAAGCAGACTGTGGCATCACAAGGATGTGCAGGACGTTCTAACAGAAAACACGGTAAGGAACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

125

Amino Acids

14.59

Weight (kDa)

6.51

Isoelectric Point (pI)

57.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 28 - 96 3.7e-15 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 293
AciI CCGC 1 cut(s) 166
AcsI RAATTY 1 cut(s) 237
AgsI TTSAA 3 cut(s) 41, 118, 155
Ama87I CYCGRG 1 cut(s) 176
ApoI RAATTY 1 cut(s) 237
AspA2I CCTAGG 1 cut(s) 29
AspLEI GCGC 1 cut(s) 211
AsuC2I CCSGG 1 cut(s) 303
AvaI CYCGRG 1 cut(s) 176
AvrII CCTAGG 1 cut(s) 29
BanII GRGCYC 1 cut(s) 303
BccI CCATC 3 cut(s) 2, 59, 259
BcnI CCSGG 1 cut(s) 303
BfaI CTAG 1 cut(s) 30
BfmI CTRYAG 1 cut(s) 294
BfoI RGCGCY 1 cut(s) 212
BlnI CCTAGG 1 cut(s) 29
Bme1390I CCNGG 1 cut(s) 303
BmeT110I CYCGRG 1 cut(s) 176
BmrFI CCNGG 1 cut(s) 303
BmrI ACTGGG 1 cut(s) 80
BmsI GCATC 1 cut(s) 334
BmuI ACTGGG 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 303
BsaJI CCNNGG 1 cut(s) 29
Bse1I ACTGG 1 cut(s) 75
Bse3DI GCAATG 1 cut(s) 102
BseDI CCNNGG 1 cut(s) 29
BseGI GGATG 1 cut(s) 340
BseMI GCAATG 1 cut(s) 102
BseNI ACTGG 1 cut(s) 75
BsgI GTGCAG 1 cut(s) 359
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 1 cut(s) 303
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 303
BspACI CCGC 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 102
BsrI ACTGG 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 29
BssT1I CCWWGG 1 cut(s) 29
Bst4CI ACNGT 2 cut(s) 321, 364
Bst6I CTCTTC 1 cut(s) 255
BstC8I GCNNGC 1 cut(s) 245
BstF5I GGATG 1 cut(s) 340
BstH2I RGCGCY 1 cut(s) 212
BstHHI GCGC 1 cut(s) 211
BstMWI GCNNNNNNNGC 1 cut(s) 307
BstNSI RCATGY 1 cut(s) 247
BstSCI CCNGG 1 cut(s) 301
BstSFI CTRYAG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 340
Cac8I GCNNGC 1 cut(s) 245
CfoI GCGC 1 cut(s) 211
CviAII CATG 2 cut(s) 244, 248
CviJI RGCY 1 cut(s) 301
CviKI_1 RGCY 1 cut(s) 301
DraI TTTAAA 1 cut(s) 16
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
Eco130I CCWWGG 1 cut(s) 29
Eco24I GRGCYC 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 29
EcoT22I ATGCAT 1 cut(s) 249
EcoT38I GRGCYC 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 29
FaeI CATG 2 cut(s) 247, 251
FaiI YATR 8 cut(s) 63, 183, 215, 217, 219, 231, 245, 249
FatI CATG 2 cut(s) 243, 247
FblI GTMKAC 1 cut(s) 293
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 1 cut(s) 303
FspBI CTAG 1 cut(s) 30
GlaI GCGC 1 cut(s) 210
HaeII RGCGCY 1 cut(s) 212
HapII CCGG 1 cut(s) 303
HhaI GCGC 1 cut(s) 211
Hin1II CATG 2 cut(s) 247, 251
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HinfI GANTC 2 cut(s) 22, 290
HpaII CCGG 1 cut(s) 303
Hpy166II GTNNAC 1 cut(s) 294
Hpy188III TCNNGA 1 cut(s) 41
Hpy8I GTNNAC 1 cut(s) 294
HpyAV CCTTC 1 cut(s) 149
HpyCH4III ACNGT 2 cut(s) 321, 364
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 4 cut(s) 243, 247, 286, 340
HpyF10VI GCNNNNNNNGC 1 cut(s) 307
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 2 cut(s) 247, 251
HspAI GCGC 1 cut(s) 209
LpnPI CCDG 3 cut(s) 56, 316, 326
LweI GCATC 1 cut(s) 334
MaeI CTAG 1 cut(s) 30
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 1 cut(s) 161
MboII GAAGA 2 cut(s) 106, 272
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 1 cut(s) 237
MlyI GAGTC 1 cut(s) 299
MmeI TCCRAC 2 cut(s) 104, 237
MnlI CCTC 2 cut(s) 36, 107
Mph1103I ATGCAT 1 cut(s) 249
MseI TTAA 3 cut(s) 15, 171, 189
MslI CAYNNNNRTG 1 cut(s) 333
MspI CCGG 1 cut(s) 303
MspR9I CCNGG 1 cut(s) 303
MwoI GCNNNNNNNGC 1 cut(s) 307
NciI CCSGG 1 cut(s) 303
NlaIII CATG 2 cut(s) 247, 251
NmuCI GTSAC 1 cut(s) 161
NsiI ATGCAT 1 cut(s) 249
NspI RCATGY 1 cut(s) 247
PaeI GCATGC 1 cut(s) 247
PfeI GAWTC 1 cut(s) 22
PleI GAGTC 1 cut(s) 298
PpsI GAGTC 1 cut(s) 298
RseI CAYNNNNRTG 1 cut(s) 333
SaqAI TTAA 3 cut(s) 15, 171, 189
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 1 cut(s) 303
SduI GDGCHC 1 cut(s) 303
SetI ASST 3 cut(s) 124, 160, 348
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 333
SphI GCATGC 1 cut(s) 247
Sse9I AATT 1 cut(s) 237
SsiI CCGC 1 cut(s) 166
SspMI CTAG 1 cut(s) 30
StyD4I CCNGG 1 cut(s) 301
StyI CCWWGG 1 cut(s) 29
TaaI ACNGT 2 cut(s) 321, 364
TaiI ACGT 1 cut(s) 348
TaqI TCGA 1 cut(s) 99
TasI AATT 1 cut(s) 237
TfiI GAWTC 1 cut(s) 22
Tru1I TTAA 3 cut(s) 15, 171, 189
Tru9I TTAA 3 cut(s) 15, 171, 189
TseFI GTSAC 1 cut(s) 161
Tsp45I GTSAC 1 cut(s) 161
XapI RAATTY 1 cut(s) 237
XceI RCATGY 1 cut(s) 247
XmaJI CCTAGG 1 cut(s) 29
XmiI GTMKAC 1 cut(s) 293
XspI CTAG 1 cut(s) 30
Zsp2I ATGCAT 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.