RchiOBHm_Chr6g0293421

HPC2 and ubinuclein domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003763
Physical Location & Seq
Forward (+)
56127866 .. 56129160
1295 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26328

Sequence Viewer

Length: 882 bp
ATGCCTATGATGATAGTACAACCTTCTGGAGAGGGAGAGGGAGAGGAAGGGAAAGATGAAGCTGCGCCGAATCGTTTTAGTGTTGTGATTGAGAAGATTGAGCGCCTATATATGGGTAAAGATAGTAGTGACGATGAAGATCAAAATATTCCTGATGACGATCAGTATGATACTGAGGACTCATTTATTGATGATGCTGAGTTGGATGAATATTTTGAAGTGGATAATTCAGCTATAAAACATGACGGATTCTTTGTTAACAGGGGCCAACTAGAACGGATAAACACAACTGCTGCATTACCTAACCAGCAACCAAAGAAAAGGAGAAGAAAAGAGGCAAAGAGCCCCGGTGAAACTGATGACTGTCACGTGCCAAATAAACAAGCAAAGTTAGGTAAGACAGCTGCAGCAAAGATTACATCGGGACTTGCAAAGAACTCATCTGCTCTCATTACTATTGCTGTAACCACGGAACACCGTGAAGATGTGGAATTTCAGAATCCATTGAACTCTTCCGGTTATTCATCCACTAAAAAGTCTGCTGATTCTAAAACAGTTATGAATCCTTCTCCGTTGAAAGCATTGGATGGAGATGCTTCTGCACTGCAAGTAGAGGTGAAGGATATTGATAAGTCTAAGTCAGGAATCCTGCTTCCTAAGGACTCGAGTAATAGATTTAAAGATTCCGGTGGGTCCTCTGATGCATCGTATCATAAATATCATGATAAAAATGCTTATTCGCAAACCAAAACCCAGTCGGGTAGACTATCTGGCAACGCTGATGAGCTGGAATCATCAGTTCGGGCGAAAGTGAAAAATGGGATCCGTCAACTGCTTGACCTTAACCTCTCGGACGGGAAGTACTCTGTTCCAACTACATAA

Protein Analysis

293

Amino Acids

32.1

Weight (kDa)

5.08

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HUN PF08729 46 - 92 7.7e-13 HPC2 and ubinuclein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 763
AclWI GGATC 2 cut(s) 817, 830
AcsI RAATTY 1 cut(s) 491
AcvI CACGTG 1 cut(s) 370
AfaI GTAC 2 cut(s) 18, 863
AfiI CCNNNNNNNGG 1 cut(s) 112
AgsI TTSAA 3 cut(s) 218, 508, 577
AluBI AGCT 4 cut(s) 62, 233, 404, 787
AluI AGCT 4 cut(s) 62, 233, 404, 787
AlwI GGATC 2 cut(s) 817, 830
Ama87I CYCGRG 1 cut(s) 664
AoxI GGCC 1 cut(s) 265
ApeKI GCWGC 4 cut(s) 62, 293, 404, 407
ApoI RAATTY 1 cut(s) 491
ArsI GACNNNNNNTTYG 2 cut(s) 236, 268
AspLEI GCGC 2 cut(s) 67, 105
AspS9I GGNCC 2 cut(s) 265, 693
AsuC2I CCSGG 1 cut(s) 348
AsuHPI GGTGA 2 cut(s) 362, 628
AvaI CYCGRG 1 cut(s) 664
AvaII GGWCC 1 cut(s) 693
AxyI CCTNAGG 1 cut(s) 657
BamHI GGATCC 1 cut(s) 822
BanII GRGCYC 1 cut(s) 347
BbrPI CACGTG 1 cut(s) 370
BbvI GCAGC 4 cut(s) 49, 280, 391, 419
BccI CCATC 1 cut(s) 581
BcnI CCSGG 1 cut(s) 348
BfaI CTAG 1 cut(s) 272
BfmI CTRYAG 1 cut(s) 405
BfoI RGCGCY 1 cut(s) 106
BisI GCNGC 4 cut(s) 63, 294, 405, 408
BlsI GCNGC 4 cut(s) 64, 295, 406, 409
BmcAI AGTACT 1 cut(s) 863
Bme1390I CCNGG 1 cut(s) 348
Bme18I GGWCC 1 cut(s) 693
BmeT110I CYCGRG 1 cut(s) 664
BmgT120I GGNCC 2 cut(s) 265, 693
BmiI GGNNCC 3 cut(s) 266, 694, 824
BmrFI CCNGG 1 cut(s) 348
BmrI ACTGGG 1 cut(s) 748
BmsI GCATC 4 cut(s) 184, 583, 691, 713
BmuI ACTGGG 1 cut(s) 748
BpmI CTGGAG 1 cut(s) 48
BpuMI CCSGG 1 cut(s) 348
BsaAI YACGTR 1 cut(s) 370
BsaBI GATNNNNATC 2 cut(s) 138, 159
BsaJI CCNNGG 2 cut(s) 346, 468
BsaWI WCCGGW 2 cut(s) 515, 686
Bsc4I CCNNNNNNNGG 1 cut(s) 112
Bse1I ACTGG 1 cut(s) 754
Bse21I CCTNAGG 1 cut(s) 657
Bse8I GATNNNNATC 2 cut(s) 138, 159
BseDI CCNNGG 2 cut(s) 346, 468
BseGI GGATG 3 cut(s) 211, 524, 592
BseJI GATNNNNATC 2 cut(s) 138, 159
BseLI CCNNNNNNNGG 1 cut(s) 112
BseMII CTCAG 2 cut(s) 165, 189
BseNI ACTGG 1 cut(s) 754
BseXI GCAGC 4 cut(s) 49, 280, 391, 419
BsgI GTGCAG 1 cut(s) 585
BshFI GGCC 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 664
BsiSI CCGG 3 cut(s) 348, 516, 687
BslFI GGGAC 1 cut(s) 438
BslI CCNNNNNNNGG 1 cut(s) 112
BsmFI GGGAC 1 cut(s) 438
BsnI GGCC 1 cut(s) 267
BsoBI CYCGRG 1 cut(s) 664
Bsp1286I GDGCHC 1 cut(s) 347
Bsp143I GATC 3 cut(s) 139, 160, 822
BspANI GGCC 1 cut(s) 267
BspCNI CTCAG 2 cut(s) 166, 190
BspHI TCATGA 1 cut(s) 721
BspLI GGNNCC 3 cut(s) 266, 694, 824
BspMAI CTGCAG 1 cut(s) 409
BspPI GGATC 2 cut(s) 817, 830
BsrI ACTGG 1 cut(s) 754
BssECI CCNNGG 2 cut(s) 346, 468
BssMI GATC 3 cut(s) 139, 160, 822
Bst4CI ACNGT 3 cut(s) 365, 479, 556
Bst6I CTCTTC 1 cut(s) 517
BstBAI YACGTR 1 cut(s) 370
BstDEI CTNAG 4 cut(s) 174, 198, 636, 657
BstDSI CCRYGG 1 cut(s) 468
BstF5I GGATG 3 cut(s) 211, 524, 592
BstH2I RGCGCY 1 cut(s) 106
BstHHI GCGC 2 cut(s) 67, 105
BstKTI GATC 3 cut(s) 142, 163, 825
BstMBI GATC 3 cut(s) 139, 160, 822
BstSCI CCNGG 1 cut(s) 346
BstSFI CTRYAG 1 cut(s) 405
BstV1I GCAGC 4 cut(s) 49, 280, 391, 419
BstX2I RGATCY 1 cut(s) 822
BstYI RGATCY 1 cut(s) 822
Bsu36I CCTNAGG 1 cut(s) 657
BsuRI GGCC 1 cut(s) 267
BtgI CCRYGG 1 cut(s) 468
BtsCI GGATG 3 cut(s) 211, 524, 592
BtsI GCAGTG 1 cut(s) 602
BtsIMutI CAGTG 1 cut(s) 602
CciI TCATGA 1 cut(s) 721
CfoI GCGC 2 cut(s) 67, 105
Cfr13I GGNCC 2 cut(s) 265, 693
Csp6I GTAC 2 cut(s) 17, 862
CviAII CATG 2 cut(s) 242, 722
CviJI RGCY 6 cut(s) 62, 233, 267, 345, 404, 787
CviKI_1 RGCY 6 cut(s) 62, 233, 267, 345, 404, 787
CviQI GTAC 2 cut(s) 17, 862
DdeI CTNAG 4 cut(s) 174, 198, 636, 657
DpnI GATC 3 cut(s) 141, 162, 824
DpnII GATC 3 cut(s) 139, 160, 822
DraI TTTAAA 1 cut(s) 679
Eam1104I CTCTTC 1 cut(s) 517
EarI CTCTTC 1 cut(s) 517
Eco24I GRGCYC 1 cut(s) 347
Eco47I GGWCC 1 cut(s) 693
Eco72I CACGTG 1 cut(s) 370
Eco81I CCTNAGG 1 cut(s) 657
Eco88I CYCGRG 1 cut(s) 664
EcoO109I RGGNCCY 1 cut(s) 693
EcoT22I ATGCAT 1 cut(s) 706
EcoT38I GRGCYC 1 cut(s) 347
FaeI CATG 2 cut(s) 245, 725
FaqI GGGAC 1 cut(s) 438
FatI CATG 2 cut(s) 241, 721
FblI GTMKAC 1 cut(s) 763
Fnu4HI GCNGC 4 cut(s) 63, 294, 405, 408
FokI GGATG 3 cut(s) 218, 511, 599
FriOI GRGCYC 1 cut(s) 347
Fsp4HI GCNGC 4 cut(s) 63, 294, 405, 408
FspBI CTAG 1 cut(s) 272
GlaI GCGC 2 cut(s) 66, 104
GluI GCNGC 4 cut(s) 63, 294, 405, 408
GsuI CTGGAG 1 cut(s) 48
HaeII RGCGCY 1 cut(s) 106
HaeIII GGCC 1 cut(s) 267
HapII CCGG 3 cut(s) 348, 516, 687
HhaI GCGC 2 cut(s) 67, 105
Hin1II CATG 2 cut(s) 245, 725
Hin6I GCGC 2 cut(s) 65, 103
HinP1I GCGC 2 cut(s) 65, 103
HincII GTYRAC 2 cut(s) 259, 830
HindII GTYRAC 2 cut(s) 259, 830
HpaI GTTAAC 1 cut(s) 259
HpaII CCGG 3 cut(s) 348, 516, 687
HphI GGTGA 2 cut(s) 362, 628
Hpy166II GTNNAC 3 cut(s) 259, 764, 830
Hpy188I TCNGA 3 cut(s) 498, 700, 853
Hpy188III TCNNGA 5 cut(s) 27, 152, 423, 642, 722
Hpy8I GTNNAC 3 cut(s) 259, 764, 830
HpyAV CCTTC 4 cut(s) 33, 41, 576, 613
HpyCH4III ACNGT 3 cut(s) 365, 479, 556
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 6 cut(s) 296, 407, 431, 602, 607, 704
HpyF3I CTNAG 4 cut(s) 174, 198, 636, 657
HpySE526I ACGT 1 cut(s) 369
Hsp92II CATG 2 cut(s) 245, 725
HspAI GCGC 2 cut(s) 65, 103
KspAI GTTAAC 1 cut(s) 259
Kzo9I GATC 3 cut(s) 139, 160, 822
Lsp1109I GCAGC 4 cut(s) 49, 280, 391, 419
LweI GCATC 4 cut(s) 184, 583, 691, 713
MaeI CTAG 1 cut(s) 272
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 3 cut(s) 128, 365, 463
MalI GATC 3 cut(s) 141, 162, 824
MboI GATC 3 cut(s) 139, 160, 822
MboII GAAGA 5 cut(s) 106, 149, 339, 494, 504
MflI RGATCY 1 cut(s) 822
MhlI GDGCHC 1 cut(s) 347
MluCI AATT 2 cut(s) 226, 491
MlyI GAGTC 2 cut(s) 173, 656
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 8 cut(s) 25, 31, 37, 169, 328, 607, 706, 857
Mph1103I ATGCAT 1 cut(s) 706
MseI TTAA 3 cut(s) 258, 678, 843
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 3 cut(s) 348, 516, 687
MspR9I CCNGG 1 cut(s) 348
NciI CCSGG 1 cut(s) 348
NdeII GATC 3 cut(s) 139, 160, 822
NlaIII CATG 2 cut(s) 245, 725
NlaIV GGNNCC 3 cut(s) 266, 694, 824
NmuCI GTSAC 2 cut(s) 128, 365
NsiI ATGCAT 1 cut(s) 706
PaeR7I CTCGAG 1 cut(s) 664
PagI TCATGA 1 cut(s) 721
PfeI GAWTC 8 cut(s) 70, 249, 499, 545, 562, 645, 683, 791
PkrI GCNGC 4 cut(s) 64, 295, 406, 409
PleI GAGTC 2 cut(s) 173, 656
PmaCI CACGTG 1 cut(s) 370
PmlI CACGTG 1 cut(s) 370
PpsI GAGTC 2 cut(s) 173, 656
Ppu21I YACGTR 1 cut(s) 370
PpuMI RGGWCCY 1 cut(s) 693
Psp5II RGGWCCY 1 cut(s) 693
PspCI CACGTG 1 cut(s) 370
PspN4I GGNNCC 3 cut(s) 266, 694, 824
PspPI GGNCC 2 cut(s) 265, 693
PspPPI RGGWCCY 1 cut(s) 693
PspXI VCTCGAGB 1 cut(s) 664
PstI CTGCAG 1 cut(s) 409
PsuI RGATCY 1 cut(s) 822
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 2 cut(s) 18, 863
RsaNI GTAC 2 cut(s) 17, 862
SaqAI TTAA 3 cut(s) 258, 678, 843
SatI GCNGC 4 cut(s) 63, 294, 405, 408
Sau3AI GATC 3 cut(s) 139, 160, 822
Sau96I GGNCC 2 cut(s) 265, 693
ScaI AGTACT 1 cut(s) 863
SchI GAGTC 2 cut(s) 173, 656
ScrFI CCNGG 1 cut(s) 348
SduI GDGCHC 1 cut(s) 347
SfaNI GCATC 4 cut(s) 184, 583, 691, 713
SfcI CTRYAG 1 cut(s) 405
Sfr274I CTCGAG 1 cut(s) 664
SinI GGWCC 1 cut(s) 693
SlaI CTCGAG 1 cut(s) 664
SmlI CTYRAG 1 cut(s) 664
SmoI CTYRAG 1 cut(s) 664
Sse9I AATT 2 cut(s) 226, 491
SspI AATATT 2 cut(s) 148, 212
SspMI CTAG 1 cut(s) 272
StyD4I CCNGG 1 cut(s) 346
TaaI ACNGT 3 cut(s) 365, 479, 556
TaiI ACGT 1 cut(s) 372
TaqI TCGA 1 cut(s) 665
TasI AATT 2 cut(s) 226, 491
TatI WGTACW 2 cut(s) 16, 861
TfiI GAWTC 8 cut(s) 70, 249, 499, 545, 562, 645, 683, 791
Tru1I TTAA 3 cut(s) 258, 678, 843
Tru9I TTAA 3 cut(s) 258, 678, 843
TscAI CASTG 1 cut(s) 609
TseFI GTSAC 2 cut(s) 128, 365
TseI GCWGC 4 cut(s) 62, 293, 404, 407
Tsp45I GTSAC 2 cut(s) 128, 365
TspDTI ATGAA 5 cut(s) 72, 150, 222, 513, 575
TspGWI ACGGA 5 cut(s) 261, 292, 485, 561, 815
TspRI CASTG 1 cut(s) 609
VpaK11BI GGWCC 1 cut(s) 693
XapI RAATTY 1 cut(s) 491
XhoI CTCGAG 1 cut(s) 664
XmiI GTMKAC 1 cut(s) 763
XspI CTAG 1 cut(s) 272
ZrmI AGTACT 1 cut(s) 863
Zsp2I ATGCAT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.