RchiOBHm_Chr6g0296701

HPC2 and ubinuclein domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003763
Physical Location & Seq
Reverse (-)
58444655 .. 58450794
6140 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26632

Sequence Viewer

Length: 2265 bp
ATGGACGACCAGAAGGCCGGCCCCGAATCCTCGAGGCCCTCCACCTCGTTTCTCAAAGCCGGCGACCGGCAAATGTTCACGGTGGAGCTCCGACCCGGCGAGACCACCATAGTCTCCTGGAAGAAGCTCGTCAGGGACACTAACAAGGTCAATGGCCTGCCTCCGCCCACTGCCACTGCCACTGCCCCTGACCCTCCCCCAACGCTCACCCTAACCTCGAGTCTCGTACAACCTTCTGGAGAGGGAGAGGGAGAGGGAGAGGAAGGGAAAGATGAAGCTGCGCCGAATCGTTTTAGTGCTGTGATTGAGAAGATTGAGCGCCTATATATGGGTAAAGATAGTAGCGACGATGAAGATCAAAATATTCCTGATGACGATCAGTATGATACTGAGGACTCTTTTATTGATGATGCTGAGTTGGATGAATATTTTGAAGTTGATAATTCAGCTATAAAACATGACGGATTCTTTGTTAACAGGGGCCAACTAGAACGGATAAACACAACTGCTGCATTACCTAACCAGCAACCAAAGAAAAGGAGAAGAAAAGAGGCAAAGAGCCCCGGTGAAACTGATGACAGTCACGTGCCAAATAAACAAGCAAAGTTAGGTAAGACAGCCGCAGCAAAGATTACATCGGGACTTGCAAAGAACTCATCTGCTCTCACTACTGTTGCTGTAACCACGGAACACCGTGAAGATGTGAAATTTCAGAATCCATTGAATTCTTCCGGTTATTCATCCACTAAAAAGTCTGCTGATTCTAAAACAGTTATGAATCCTTCTCCTTTGAAAGCATTGGATGGAGATGCTTCTGCACTGCAAGCAGAGGTGAAGGATATTGATAAGTCTAAGTCAGGAATCCTGCTTCCTAAGGACTCGAGTAATAGATTTAAAGATGCCGGTGGGTCCTCTGATGCATCGTATCATAAGTATCATGATAAAAATGCTTATTCGCAAACTAAAACCCAGTCGGGTAGACTATCTGGCAACGCTGATGAGCTGGAATCATCAGTTCGGGCGAAAGTGAAAAATGGGATCCGTCAACTGCCTGACCTTAACCTCTCGGACGGGAAGTACTCTGTTCCAACTACAAAAACTTCACATTTGCATCGAAAGGAGGGTTCTAGTGTAAGGCCCAAGGGTTCGATGCTTGAAAAGGCTATTAGGGAGTTGGAGCGGATGGTTGCAGAATCAAGGCCACCTGCTATGGAGAATGCAGAGGGAGATAATTCATCCCAAGCAATTAAAAGGAGATTGCCTAGAGAAATAAAGATGAAGCTTGCTAAAGTTGCTAGACTAGCGCAGGCTAGCCATGGAAAAATATCAAAAGAGTTACTTAACCGGCTTATGAGTATTCTTGGCCACTTAATACAGCTCAGGACGTTAAAGAGAAATTTAAAGGTTATGATTAGTATGGGTCTATCAGCCAAAAAGGAGAAAGATGACAGGTTTCAACAGATCAAGAAGGAAGTTATAGATATGGTTAAGATAAATGTATCCTCTTTGGAGTCTAAGGCATTGGAACAAACTGGAGCCTCCGATGATTTTCAAGAAATAGCCTCTGGAGCAAAAGAAGTCTCTAAAAGAAAGTTGAGCATGGATCCTGTATTAGAGGACAAGATTTGTGACCTCTATGACCTTTATGTTGATGGGCTGGATGAAGATATAGGTCCACAAATAAGAAAGCTCTATGCTGAGCTAGCGGGATTGTGGCCTAGTGGTTTCATGGACAATCATGGCATCAAAAGTGCAATTTGTCGGGCAAAAGATAGACGGAGGGAGCGATACAGCCAAAATAAAGATCAGGAGAAAATGAGGAGGAAAAAGATGTTGACTCCAAAATTAGAGGAGTCTGTTAGAGTTGACGCAAGTTCAATTCCCCAGCAACAGTATATACGAGAGAGAGTGGCAACTGAACCTAGCAGTCATGGTTCAGGGAACAAGACTGTATCAGGTACAACAGCAGCTGTGAGGATACCCAGTCCAATAAACGGTCCTAGCTTTGACAGGCTTAAACAAGAGAAGCTCAAGGGAAGTGCAAGTAATTCCCCTGATGACACGAGGGTCGCAGATGGTGCATTGGTAAAGAAAAAGGTGAAGAGGAAGCCAGATCAAGAGTTGGATGAAACTCGGATACGGCCAGAAAAATTACCTTCCCAACAAGGTGAGGAAAGACAAAAGTCATTCAAGCAGGCGGCTGGTGTACCCCAGAAGTCAAACCATCAATCCACCGGCCTTCCTAGCGCTGAGCAGTCTAGCTGA

Protein Analysis

754

Amino Acids

83.19

Weight (kDa)

9.13

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HUN PF08729 118 - 164 2.8e-12 HPC2 and ubinuclein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1213
AasI GACNNNNNNGTC 1 cut(s) 2066
AbsI CCTCGAGG 1 cut(s) 31
Acc36I ACCTGC 1 cut(s) 1213
AccBSI CCGCTC 1 cut(s) 1180
AccI GTMKAC 1 cut(s) 979
AciI CCGC 5 cut(s) 164, 621, 1180, 1706, 2198
AclWI GGATC 4 cut(s) 1033, 1046, 1598, 1611
AcoI YGGCCR 2 cut(s) 1363, 2141
AcsI RAATTY 3 cut(s) 707, 724, 1396
AcvI CACGTG 1 cut(s) 586
AfaI GTAC 4 cut(s) 228, 1079, 1960, 2208
AfeI AGCGCT 1 cut(s) 2248
AfiI CCNNNNNNNGG 3 cut(s) 66, 328, 1994
AgsI TTSAA 8 cut(s) 434, 724, 793, 1157, 1457, 1553, 1878, 2191
AjnI CCWGG 1 cut(s) 116
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 4 cut(s) 95, 118, 227, 1585
AlwI GGATC 4 cut(s) 1033, 1046, 1598, 1611
AlwNI CAGNNNCTG 1 cut(s) 1970
Ama87I CYCGRG 3 cut(s) 31, 217, 880
Aor51HI AGCGCT 1 cut(s) 2248
ApeKI GCWGC 4 cut(s) 278, 509, 623, 1967
ApoI RAATTY 3 cut(s) 707, 724, 1396
ArsI GACNNNNNNTTYG 2 cut(s) 452, 484
AspLEI GCGC 4 cut(s) 283, 321, 1306, 2249
AspS9I GGNCC 7 cut(s) 20, 36, 481, 909, 1137, 1673, 1997
AsuC2I CCSGG 2 cut(s) 96, 564
AsuHPI GGTGA 5 cut(s) 199, 578, 844, 2110, 2180
AsuNHI GCTAGC 2 cut(s) 1310, 1702
AvaI CYCGRG 3 cut(s) 31, 217, 880
AvaII GGWCC 3 cut(s) 909, 1673, 1997
AxyI CCTNAGG 1 cut(s) 873
BalI TGGCCA 1 cut(s) 1365
BamHI GGATCC 2 cut(s) 1038, 1603
BanII GRGCYC 2 cut(s) 90, 563
BauI CACGAG 1 cut(s) 2062
BbrPI CACGTG 1 cut(s) 586
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 4 cut(s) 265, 496, 635, 1979
BccI CCATC 5 cut(s) 797, 1177, 1646, 2069, 2232
BceAI ACGGC 1 cut(s) 2156
BciT130I CCWGG 1 cut(s) 118
BciVI GTATCC 3 cut(s) 1510, 1971, 2130
BcnI CCSGG 2 cut(s) 96, 564
BcoDI GTCTC 4 cut(s) 95, 118, 227, 1585
BfoI RGCGCY 2 cut(s) 322, 2250
BfuAI ACCTGC 1 cut(s) 1213
BfuI GTATCC 3 cut(s) 1510, 1971, 2130
BisI GCNGC 6 cut(s) 279, 510, 621, 624, 1968, 2199
BlpI GCTNAGC 2 cut(s) 1698, 2250
BlsI GCNGC 6 cut(s) 280, 511, 622, 625, 1969, 2200
BmcAI AGTACT 1 cut(s) 1079
Bme1390I CCNGG 3 cut(s) 96, 118, 564
Bme18I GGWCC 3 cut(s) 909, 1673, 1997
BmeT110I CYCGRG 3 cut(s) 31, 217, 880
BmgT120I GGNCC 7 cut(s) 20, 36, 481, 909, 1137, 1673, 1997
BmiI GGNNCC 6 cut(s) 22, 482, 910, 1040, 1537, 1605
BmrFI CCNGG 3 cut(s) 96, 118, 564
BmrI ACTGGG 2 cut(s) 964, 1977
BmsI GCATC 8 cut(s) 400, 799, 889, 907, 929, 1120, 1140, 1752
BmtI GCTAGC 2 cut(s) 1314, 1706
BmuI ACTGGG 2 cut(s) 964, 1977
BoxI GACNNNNGTC 1 cut(s) 2182
BpmI CTGGAG 3 cut(s) 258, 1554, 1587
Bpu10I CCTNAGC 1 cut(s) 1379
Bpu1102I GCTNAGC 2 cut(s) 1698, 2250
BpuEI CTTGAG 1 cut(s) 2015
BpuMI CCSGG 2 cut(s) 96, 564
BsaAI YACGTR 1 cut(s) 586
BsaBI GATNNNNATC 2 cut(s) 354, 375
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 4 cut(s) 562, 684, 1140, 1315
BsaWI WCCGGW 1 cut(s) 731
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 328, 1994
Bse118I RCCGGY 6 cut(s) 17, 59, 66, 902, 1344, 2234
Bse1I ACTGG 3 cut(s) 970, 1537, 1983
Bse21I CCTNAGG 1 cut(s) 873
Bse8I GATNNNNATC 2 cut(s) 354, 375
BseBI CCWGG 1 cut(s) 118
BseDI CCNNGG 4 cut(s) 562, 684, 1140, 1315
BseGI GGATG 7 cut(s) 427, 740, 808, 1188, 1235, 1666, 2131
BseJI GATNNNNATC 2 cut(s) 354, 375
BseLI CCNNNNNNNGG 3 cut(s) 66, 328, 1994
BseMII CTCAG 5 cut(s) 381, 405, 1393, 1689, 2241
BseNI ACTGG 3 cut(s) 970, 1537, 1983
BseRI GAGGAG 2 cut(s) 1834, 1865
BseXI GCAGC 4 cut(s) 265, 496, 635, 1979
BseYI CCCAGC 1 cut(s) 1884
BsgI GTGCAG 1 cut(s) 801
Bsh1285I CGRYCG 1 cut(s) 67
BsiEI CGRYCG 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 90
BsiHKCI CYCGRG 3 cut(s) 31, 217, 880
BsiSI CCGG 9 cut(s) 18, 60, 67, 96, 564, 732, 903, 1345, 2235
BslFI GGGAC 2 cut(s) 149, 654
BslI CCNNNNNNNGG 3 cut(s) 66, 328, 1994
BsmAI GTCTC 4 cut(s) 95, 118, 227, 1585
BsmFI GGGAC 2 cut(s) 149, 654
BsmI GAATGC 1 cut(s) 1222
Bso31I GGTCTC 1 cut(s) 95
BsoBI CYCGRG 3 cut(s) 31, 217, 880
Bsp1286I GDGCHC 2 cut(s) 90, 563
Bsp143I GATC 7 cut(s) 355, 376, 1038, 1461, 1603, 1804, 2113
Bsp1720I GCTNAGC 2 cut(s) 1698, 2250
Bsp19I CCATGG 1 cut(s) 1315
BspACI CCGC 5 cut(s) 164, 621, 1180, 1706, 2198
BspCNI CTCAG 5 cut(s) 382, 406, 1392, 1690, 2242
BspHI TCATGA 1 cut(s) 937
BspLI GGNNCC 6 cut(s) 22, 482, 910, 1040, 1537, 1605
BspMI ACCTGC 1 cut(s) 1213
BspOI GCTAGC 2 cut(s) 1314, 1706
BspPI GGATC 4 cut(s) 1033, 1046, 1598, 1611
BspTNI GGTCTC 1 cut(s) 95
BsrBI CCGCTC 1 cut(s) 1180
BsrFI RCCGGY 6 cut(s) 17, 59, 66, 902, 1344, 2234
BsrI ACTGG 3 cut(s) 970, 1537, 1983
BssAI RCCGGY 6 cut(s) 17, 59, 66, 902, 1344, 2234
BssECI CCNNGG 4 cut(s) 562, 684, 1140, 1315
BssMI GATC 7 cut(s) 355, 376, 1038, 1461, 1603, 1804, 2113
BssSI CACGAG 1 cut(s) 2062
BssT1I CCWWGG 2 cut(s) 1140, 1315
Bst2BI CACGAG 1 cut(s) 2062
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 8 cut(s) 82, 581, 673, 695, 772, 1893, 1951, 1997
Bst6I CTCTTC 1 cut(s) 2096
BstAPI GCANNNNNTGC 1 cut(s) 2078
BstBAI YACGTR 1 cut(s) 586
BstC8I GCNNGC 9 cut(s) 19, 61, 158, 825, 1284, 1308, 1312, 1704, 2196
BstDEI CTNAG 8 cut(s) 390, 414, 852, 873, 1379, 1515, 1698, 2250
BstDSI CCRYGG 2 cut(s) 684, 1315
BstF5I GGATG 7 cut(s) 427, 740, 808, 1188, 1235, 1666, 2131
BstH2I RGCGCY 2 cut(s) 322, 2250
BstHHI GCGC 4 cut(s) 283, 321, 1306, 2249
BstKTI GATC 7 cut(s) 358, 379, 1041, 1464, 1606, 1807, 2116
BstMAI GTCTC 4 cut(s) 95, 118, 227, 1585
BstMBI GATC 7 cut(s) 355, 376, 1038, 1461, 1603, 1804, 2113
BstMCI CGRYCG 1 cut(s) 67
BstMWI GCNNNNNNNGC 7 cut(s) 824, 1292, 1301, 1568, 1703, 2078, 2244
BstNI CCWGG 1 cut(s) 118
BstPAI GACNNNNGTC 1 cut(s) 2182
BstSCI CCNGG 3 cut(s) 94, 116, 562
BstV1I GCAGC 4 cut(s) 265, 496, 635, 1979
BstX2I RGATCY 2 cut(s) 1038, 1603
BstYI RGATCY 2 cut(s) 1038, 1603
Bsu36I CCTNAGG 1 cut(s) 873
BsuI GTATCC 3 cut(s) 1510, 1971, 2130
BtgI CCRYGG 2 cut(s) 684, 1315
BtsCI GGATG 7 cut(s) 427, 740, 808, 1188, 1235, 1666, 2131
BtsI GCAGTG 4 cut(s) 168, 174, 180, 818
BtsIMutI CAGTG 4 cut(s) 168, 174, 180, 818
BveI ACCTGC 1 cut(s) 1213
Cac8I GCNNGC 9 cut(s) 19, 61, 158, 825, 1284, 1308, 1312, 1704, 2196
CaiI CAGNNNCTG 1 cut(s) 1970
CciI TCATGA 1 cut(s) 937
CfoI GCGC 4 cut(s) 283, 321, 1306, 2249
Cfr10I RCCGGY 6 cut(s) 17, 59, 66, 902, 1344, 2234
Cfr13I GGNCC 7 cut(s) 20, 36, 481, 909, 1137, 1673, 1997
CseI GACGC 1 cut(s) 1877
Csp6I GTAC 4 cut(s) 227, 1078, 1959, 2207
CviAII CATG 7 cut(s) 458, 938, 1316, 1600, 1729, 1739, 1931
CviQI GTAC 4 cut(s) 227, 1078, 1959, 2207
DdeI CTNAG 8 cut(s) 390, 414, 852, 873, 1379, 1515, 1698, 2250
DpnI GATC 7 cut(s) 357, 378, 1040, 1463, 1605, 1806, 2115
DpnII GATC 7 cut(s) 355, 376, 1038, 1461, 1603, 1804, 2113
DraI TTTAAA 2 cut(s) 895, 1401
DrdI GACNNNNNNGTC 1 cut(s) 2066
DseDI GACNNNNNNGTC 1 cut(s) 2066
EaeI YGGCCR 2 cut(s) 1363, 2141
Eam1104I CTCTTC 1 cut(s) 2096
EarI CTCTTC 1 cut(s) 2096
EciI GGCGGA 1 cut(s) 153
Ecl136II GAGCTC 1 cut(s) 88
Eco130I CCWWGG 2 cut(s) 1140, 1315
Eco24I GRGCYC 2 cut(s) 90, 563
Eco31I GGTCTC 1 cut(s) 95
Eco47I GGWCC 3 cut(s) 909, 1673, 1997
Eco47III AGCGCT 1 cut(s) 2248
Eco53kI GAGCTC 1 cut(s) 88
Eco72I CACGTG 1 cut(s) 586
Eco81I CCTNAGG 1 cut(s) 873
Eco88I CYCGRG 3 cut(s) 31, 217, 880
EcoICRI GAGCTC 1 cut(s) 88
EcoO109I RGGNCCY 2 cut(s) 36, 909
EcoRI GAATTC 1 cut(s) 724
EcoRII CCWGG 1 cut(s) 116
EcoT14I CCWWGG 2 cut(s) 1140, 1315
EcoT22I ATGCAT 1 cut(s) 922
EcoT38I GRGCYC 2 cut(s) 90, 563
ErhI CCWWGG 2 cut(s) 1140, 1315
FaeI CATG 7 cut(s) 461, 941, 1319, 1603, 1732, 1742, 1934
FalI AAGNNNNNCTT 2 cut(s) 1323, 1355
FaqI GGGAC 2 cut(s) 149, 654
FatI CATG 7 cut(s) 457, 937, 1315, 1599, 1728, 1738, 1930
FauI CCCGC 1 cut(s) 1699
FblI GTMKAC 1 cut(s) 979
Fnu4HI GCNGC 6 cut(s) 279, 510, 621, 624, 1968, 2199
FokI GGATG 7 cut(s) 434, 727, 815, 1195, 1222, 1673, 2138
FriOI GRGCYC 2 cut(s) 90, 563
FseI GGCCGGCC 1 cut(s) 21
Fsp4HI GCNGC 6 cut(s) 279, 510, 621, 624, 1968, 2199
GlaI GCGC 4 cut(s) 282, 320, 1305, 2248
GluI GCNGC 6 cut(s) 279, 510, 621, 624, 1968, 2199
GsaI CCCAGC 1 cut(s) 1888
GsuI CTGGAG 3 cut(s) 258, 1554, 1587
HaeII RGCGCY 2 cut(s) 322, 2250
HapII CCGG 9 cut(s) 18, 60, 67, 96, 564, 732, 903, 1345, 2235
HgaI GACGC 1 cut(s) 1877
HhaI GCGC 4 cut(s) 283, 321, 1306, 2249
Hin1II CATG 7 cut(s) 461, 941, 1319, 1603, 1732, 1742, 1934
Hin6I GCGC 4 cut(s) 281, 319, 1304, 2247
HinP1I GCGC 4 cut(s) 281, 319, 1304, 2247
HincII GTYRAC 4 cut(s) 475, 1046, 1836, 1867
HindII GTYRAC 4 cut(s) 475, 1046, 1836, 1867
HindIII AAGCTT 1 cut(s) 1280
HpaI GTTAAC 1 cut(s) 475
HpaII CCGG 9 cut(s) 18, 60, 67, 96, 564, 732, 903, 1345, 2235
HphI GGTGA 5 cut(s) 199, 578, 844, 2110, 2180
Hpy166II GTNNAC 8 cut(s) 78, 475, 980, 1046, 1676, 1836, 1867, 2207
Hpy188I TCNGA 6 cut(s) 92, 714, 916, 1069, 1543, 2136
Hpy8I GTNNAC 8 cut(s) 78, 475, 980, 1046, 1676, 1836, 1867, 2207
Hpy99I CGWCG 1 cut(s) 350
HpyAV CCTTC 8 cut(s) 7, 243, 257, 792, 829, 1462, 2166, 2249
HpyCH4III ACNGT 8 cut(s) 82, 581, 673, 695, 772, 1893, 1951, 1997
HpyCH4IV ACGT 2 cut(s) 585, 1385
HpyF10VI GCNNNNNNNGC 7 cut(s) 824, 1292, 1301, 1568, 1703, 2078, 2244
HpyF3I CTNAG 8 cut(s) 390, 414, 852, 873, 1379, 1515, 1698, 2250
HpySE526I ACGT 2 cut(s) 585, 1385
Hsp92II CATG 7 cut(s) 461, 941, 1319, 1603, 1732, 1742, 1934
HspAI GCGC 4 cut(s) 281, 319, 1304, 2247
KroI GCCGGC 2 cut(s) 17, 59
KroNI GCCGGC 2 cut(s) 19, 61
KspAI GTTAAC 1 cut(s) 475
Kzo9I GATC 7 cut(s) 355, 376, 1038, 1461, 1603, 1804, 2113
LmnI GCTCC 6 cut(s) 85, 93, 1177, 1535, 1568, 1783
Lsp1109I GCAGC 4 cut(s) 265, 496, 635, 1979
LweI GCATC 8 cut(s) 400, 799, 889, 907, 929, 1120, 1140, 1752
MaeII ACGT 2 cut(s) 585, 1385
MaeIII GTNAC 4 cut(s) 581, 679, 1335, 1628
MalI GATC 7 cut(s) 357, 378, 1040, 1463, 1605, 1806, 2115
MbiI CCGCTC 1 cut(s) 1180
MboI GATC 7 cut(s) 355, 376, 1038, 1461, 1603, 1804, 2113
MboII GAAGA 8 cut(s) 133, 322, 365, 555, 710, 720, 1676, 2113
MflI RGATCY 2 cut(s) 1038, 1603
MhlI GDGCHC 2 cut(s) 90, 563
MlsI TGGCCA 1 cut(s) 1365
MluNI TGGCCA 1 cut(s) 1365
MlyI GAGTC 6 cut(s) 229, 389, 872, 1520, 1831, 1862
MmeI TCCRAC 5 cut(s) 115, 399, 1112, 1155, 2103
Mox20I TGGCCA 1 cut(s) 1365
Mph1103I ATGCAT 1 cut(s) 922
MroNI GCCGGC 2 cut(s) 17, 59
MscI TGGCCA 1 cut(s) 1365
Msp20I TGGCCA 1 cut(s) 1365
MspA1I CMGCKG 1 cut(s) 1970
MspI CCGG 9 cut(s) 18, 60, 67, 96, 564, 732, 903, 1345, 2235
MspR9I CCNGG 3 cut(s) 96, 118, 564
Mva1269I GAATGC 1 cut(s) 1222
MvaI CCWGG 1 cut(s) 118
MwoI GCNNNNNNNGC 7 cut(s) 824, 1292, 1301, 1568, 1703, 2078, 2244
NaeI GCCGGC 2 cut(s) 19, 61
NciI CCSGG 2 cut(s) 96, 564
NcoI CCATGG 1 cut(s) 1315
NdeII GATC 7 cut(s) 355, 376, 1038, 1461, 1603, 1804, 2113
NgoMIV GCCGGC 2 cut(s) 17, 59
NheI GCTAGC 2 cut(s) 1310, 1702
NlaIII CATG 7 cut(s) 461, 941, 1319, 1603, 1732, 1742, 1934
NlaIV GGNNCC 6 cut(s) 22, 482, 910, 1040, 1537, 1605
NmuCI GTSAC 2 cut(s) 581, 1628
NsiI ATGCAT 1 cut(s) 922
PaeR7I CTCGAG 3 cut(s) 31, 217, 880
PagI TCATGA 1 cut(s) 937
PaqCI CACCTGC 1 cut(s) 1213
PcsI WCGNNNNNNNCGW 1 cut(s) 1783
PctI GAATGC 1 cut(s) 1222
PdiI GCCGGC 2 cut(s) 19, 61
PfeI GAWTC 9 cut(s) 26, 286, 465, 715, 761, 778, 861, 1007, 1193
PfoI TCCNGGA 1 cut(s) 116
PkrI GCNGC 6 cut(s) 280, 511, 622, 625, 1969, 2200
PleI GAGTC 6 cut(s) 228, 389, 872, 1519, 1831, 1861
PmaCI CACGTG 1 cut(s) 586
PmlI CACGTG 1 cut(s) 586
PpsI GAGTC 6 cut(s) 228, 389, 872, 1519, 1831, 1861
Ppu21I YACGTR 1 cut(s) 586
PpuMI RGGWCCY 1 cut(s) 909
PshAI GACNNNNGTC 1 cut(s) 2182
Psp124BI GAGCTC 1 cut(s) 90
Psp5II RGGWCCY 1 cut(s) 909
Psp6I CCWGG 1 cut(s) 116
PspCI CACGTG 1 cut(s) 586
PspFI CCCAGC 1 cut(s) 1884
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 6 cut(s) 22, 482, 910, 1040, 1537, 1605
PspPI GGNCC 7 cut(s) 20, 36, 481, 909, 1137, 1673, 1997
PspPPI RGGWCCY 1 cut(s) 909
PspXI VCTCGAGB 3 cut(s) 31, 217, 880
PstNI CAGNNNCTG 1 cut(s) 1970
PsuI RGATCY 2 cut(s) 1038, 1603
PvuII CAGCTG 1 cut(s) 1970
RigI GGCCGGCC 1 cut(s) 21
RsaI GTAC 4 cut(s) 228, 1079, 1960, 2208
RsaNI GTAC 4 cut(s) 227, 1078, 1959, 2207
SacI GAGCTC 1 cut(s) 90
SatI GCNGC 6 cut(s) 279, 510, 621, 624, 1968, 2199
Sau3AI GATC 7 cut(s) 355, 376, 1038, 1461, 1603, 1804, 2113
Sau96I GGNCC 7 cut(s) 20, 36, 481, 909, 1137, 1673, 1997
ScaI AGTACT 1 cut(s) 1079
SchI GAGTC 6 cut(s) 229, 389, 872, 1520, 1831, 1862
ScrFI CCNGG 3 cut(s) 96, 118, 564
SduI GDGCHC 2 cut(s) 90, 563
SfaNI GCATC 8 cut(s) 400, 799, 889, 907, 929, 1120, 1140, 1752
Sfr274I CTCGAG 3 cut(s) 31, 217, 880
SinI GGWCC 3 cut(s) 909, 1673, 1997
SlaI CTCGAG 3 cut(s) 31, 217, 880
SmlI CTYRAG 4 cut(s) 31, 217, 880, 2030
SmoI CTYRAG 4 cut(s) 31, 217, 880, 2030
SsiI CCGC 5 cut(s) 164, 621, 1180, 1706, 2198
SspI AATATT 2 cut(s) 364, 428
SstI GAGCTC 1 cut(s) 90
StyD4I CCNGG 3 cut(s) 94, 116, 562
StyI CCWWGG 2 cut(s) 1140, 1315
TaaI ACNGT 8 cut(s) 82, 581, 673, 695, 772, 1893, 1951, 1997
TaiI ACGT 2 cut(s) 588, 1388
TaqI TCGA 5 cut(s) 32, 218, 881, 1114, 1148
TatI WGTACW 1 cut(s) 1077
TauI GCSGC 2 cut(s) 623, 2201
TfiI GAWTC 9 cut(s) 26, 286, 465, 715, 761, 778, 861, 1007, 1193
TscAI CASTG 4 cut(s) 175, 181, 187, 825
TseFI GTSAC 2 cut(s) 581, 1628
TseI GCWGC 4 cut(s) 278, 509, 623, 1967
Tsp45I GTSAC 2 cut(s) 581, 1628
TspGWI ACGGA 5 cut(s) 477, 508, 701, 1031, 1792
TspRI CASTG 4 cut(s) 175, 181, 187, 825
VpaK11BI GGWCC 3 cut(s) 909, 1673, 1997
XapI RAATTY 3 cut(s) 707, 724, 1396
XhoI CTCGAG 3 cut(s) 31, 217, 880
XmiI GTMKAC 1 cut(s) 979
ZrmI AGTACT 1 cut(s) 1079
Zsp2I ATGCAT 1 cut(s) 922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.