Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0004477

Physical Location & Seq

Genomic Coordinates Forward (+)

6438416 .. 6443402

Gene Structure 4987 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ16651

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2337 bp

ATGGGTGAAGCTGAGAAAGGTTCAGTGATCAAAGAGAGGCTTAAGGGTCTTTGTGTTACTAATGGGTGGTCTTATGGGGTTTTGTGGCGTTTTGATCAGAGAAATTCCATGTTGTTGACTATGGGAGAAGCCTATTATGAAGAGCACATGGGAGCAGTGATTGAAAATATGCTTCCACAGGTCCACATGCTTGGAGAAGGTGTTATAGGTAAAACTGCTTTCACTGGGAAGCACCAATGGATGCATTCAGATGCTTACAATGGACAGTGGAATTCATGCAATCCCATCGAAAGTTGTCGAGATTTGTTTCAGGATGATGCCAAGTTTCGTTGCCAATTTTCCTCTGGTATAAAGACAATTGCAACAGTCTCTGTGGAATCAGGAGTAATTCAGTTTGGTTCCACTGAAAAGATTATGGAGAGGTTGGAGTTTTTGGATGAAACAAAAAGGGTGTTCCGGGAGATCAAAAATCTTCATGGGCTTATTCCTCTAGCAAGTACACCCTCATGTTTGAACAATGAATCCAATGATCTAAATGAATTGTTTGCTTCCTTAATTTCATCTGCAAACTTTCATGGCGACGTTGCCATCATGCTTGGGGATAAATCGAAAGATGTAGGAGGAAATTGTTTATCTGCGATGAATTTACAGTCTGCAGCATCTGATGACATTGGCTCAGCTGATAAAACCCCGTGGTTTAGTACATGGAACTCTGATTCTTCTTTGTTGGCTTCATATGACTCACATTTGGCATCTGATATTGGGGTTCAGGATTCTCTCAAGACTTGCAGAAATGCAGCTCAAGACTCAACTCTCATTCAAGGCAGCTGCGGATACCAGATGGATAACCAGCAATATCTACATGGATACCCTGTAGAGTTTAACCGAACTGATTGTTTTACAGATGTTTCCAAATCATTCATGGATGATCTTTCTCAGTGGTTTGCTGCTTCACCGGAACAGAACTTCAATGGCATGGCAACCACAATGAATGGTGACCTCTCGCAAGTGTTTGAAAGTACTTCGGTCTCACCTAGTCTGGTTAAAGGTGATACGATTATCAATGTACCAATTGAGCATCCAGCAACTTCTATGCAAAGTTCCATCACGAATGCATTCAGTGCTGATGCTCATGAGAAATCTGTGATCATTCAGAGTGCTGGAAATGATTTGTTTCATGGTCTGGGATCAGATTTCGGATGTGGTCAAGTCAGCGATTGTTGGGAAGAGAGTATAATGCCGGTAGTTAGTGGTGGCTGCTTGGCTGCCGGTACTGATTTGACCAAAGAGCCAGAAGTTGGTTCTGCTGCTGGCCCCCGGAGAGGGTTATTCTCAGAATTAGGACTAGAAGAGCTTTTAAATGGTATGAGTACTACCAGCTCTGTGACTAAATGCAGTGTAGAAGACCAATCATCCACCAGGAAAAGGAAATTAGAAAGTTCATCTGTTGGCAGTAATCAGGTACAGTTAGCAAGGCTTGCTGGCTCTGGAGGGAGCATGCATTTGACGGGACCTTTGTATAACTTGGACAGGACAAACTGTCTTGTACCAAAGAAAGAGGTTCTCCCAAAATCACAGGTGGGCTTATGGATCGATGATAGCTACAGTGTCAATGCTAGAAGTGCGGCTCAAGCAAAACCACAGAAGGCTGAGGATTACACAAAGACCGCCAAGAAGAGGGCAAAGCCTGGAGAGAGTACTCGACCAAGGCCCAAGGATCGGCAGCAGATCCAAGACCGTATGAAAGACTTAAGGGGGATGATTCCCAATGGTGGCAAGTGTAGCATTGATGCTTTGCTGGACCGCACCATCAAGTACATGATTTTCTTACAAAGTGTGACAAAACATGCTGATAAGCTTAAACAACCTCATGAGTCAAAGCTGACTGGAAAGGAGAATGGTGTGGTTCTAAAAGATCAGAGCAGTAGATCTGGTGGTAACAATACTTGGGCGTTAGCAGTTGAGGGTCAAAATGTGGTTTGCCCTATACTCGTCGAGGACCTTAGTACACCTGGCCAAATGCTTATTGAGATCCTCTGTGAAGAGCGTGGTTTCTTTCTCGAGTTAGCAGACGAAATACGGGGCTTCGGGTTGAATATCTTGAAGGGGGTGATGGAGAGTCGGGAGGATAAGATATGGGCACGCTTTATCGTTGAGACAACTAGGCATGTAACCAGAATAGAAGTGTTTTGGTCCCTTGTCCGGCTTCTTCAGCAAAGTGATACCAGTGTGGTTGATCCAACCAGTCGGCCCAGCAATGTAGTAGACAACGGTTTTCCTCCTGTGTTGGATAGTTTCCCGCCACGTAGTTTTCCTCCTCCCATCAGCTTGACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0003006 GO:0003674 GO:0003700 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010033 GO:0010087 GO:0010089 GO:0010154 GO:0010468 GO:0010556 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022622 GO:0023052 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0048232 GO:0048285 GO:0048316 GO:0048364 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051321 GO:0051704 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0090058 GO:0099402 GO:0140013 GO:0140096 GO:0140110 GO:1901564 GO:1903046 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

bHLH-MYC_N

Protein Analysis

778

Amino Acids

85.28

Weight (kDa)

5.34

Isoelectric Point (pI)

40.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH-MYC_N	PF14215	11 - 48	5.6e-06	bHLH-MYC and R2R3-MYB transcription factors N-terminal
bHLH-MYC_N	PF14215	57 - 152	6.3e-17	bHLH-MYC and R2R3-MYB transcription factors N-terminal
bHLH_LHW	PF23176	568 - 628	2.8e-29	Transcription factor LHW bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004477)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64625 AT1G64625 AT1G64625 AT1G64625 AT5G24352 AT5G24352
fragaria_vesca	FvH4_4g16070 FvH4_4g16070 FvH4_4g16070 FvH4_5g11300 FvH4_5g11300 FvH4_5g11300 FvH4_5g11300 FvH4_5g11300
malus_domestica	MD06G1114400.v1.1 MD14G1135600.v1.1
prunus_persica	Prupe.5G127100_v2.0.a1
pyrus_communis	pycom06g10710 pycom14g10880 pycom14g10890
rosa_chinensis	RchiOBHm_Chr7g0186541
rosa_laevigata	RLG00000004836
rosa_multiflora	Rmu_sc0000645.1_g000021
rosa_roxburghii	Rroxscaffold_3G00268060
rosa_rugosa	Rorug06G0475000 Rorug06G0475000
rosa_samantha	Rh7AG066100 Rh7BG082000 Rh7CG081700 Rh7DG082700
rosa_wichuraiana	Rw7G006870 Rw7G007090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1298
AccI	GTMKAC	1 cut(s)	2265
AciI	CCGC	5 cut(s)	831, 1625, 1668, 1804, 2300
AclWI	GGATC	6 cut(s)	1195, 1598, 1723, 1725, 2026, 2231
AcoI	YGGCCR	1 cut(s)	2014
AcsI	RAATTY	3 cut(s)	103, 271, 643
AcuI	CTGAAG	1 cut(s)	2195
AfiI	CCNNNNNNNGG	8 cut(s)	1298, 1322, 1323, 1425, 1677, 1719, 1772, 2202
AflII	CTTAAG	2 cut(s)	41, 1750
AgsI	TTSAA	7 cut(s)	164, 514, 821, 970, 1016, 2095, 2104
AhdI	GACNNNNNGTC	1 cut(s)	1539
AjnI	CCWGG	3 cut(s)	1418, 1687, 2011
AloI	GAACNNNNNNTCC	2 cut(s)	506, 538
Alw21I	GWGCWC	1 cut(s)	147
Alw26I	GTCTC	3 cut(s)	373, 1033, 2150
AlwI	GGATC	6 cut(s)	1195, 1598, 1723, 1725, 2026, 2231
AlwNI	CAGNNNCTG	2 cut(s)	371, 662
Ama87I	CYCGRG	1 cut(s)	2060
AoxI	GGCC	4 cut(s)	1312, 1709, 2014, 2249
ApeKI	GCWGC	9 cut(s)	656, 797, 825, 828, 947, 1257, 1265, 1307, 1723
ApoI	RAATTY	3 cut(s)	103, 271, 643
ArsI	GACNNNNNNTTYG	2 cut(s)	1498, 1530
Asp700I	GAANNNNTTC	1 cut(s)	1115
AspS9I	GGNCC	8 cut(s)	181, 1313, 1511, 1710, 1801, 1999, 2193, 2250
AsuC2I	CCSGG	2 cut(s)	458, 1318
AsuHPI	GGTGA	6 cut(s)	17, 945, 1007, 1023, 1061, 2122
AvaI	CYCGRG	1 cut(s)	2060
AvaII	GGWCC	5 cut(s)	181, 1511, 1801, 1999, 2193
BaeGI	GKGCMC	1 cut(s)	2143
BaeI	ACNNNNGTAYC	2 cut(s)	2214, 2247
BalI	TGGCCA	1 cut(s)	2016
BbsI	GAAGAC	1 cut(s)	1410
Bbv12I	GWGCWC	1 cut(s)	147
BbvCI	CCTCAGC	1 cut(s)	1650
BbvI	GCAGC	9 cut(s)	668, 809, 815, 837, 934, 1244, 1252, 1294, 1735
BccI	CCATC	7 cut(s)	293, 596, 835, 1112, 1817, 2107, 2330
BcgI	CGANNNNNNTGC	2 cut(s)	268, 302
BciT130I	CCWGG	3 cut(s)	1420, 1689, 2013
BciVI	GTATCC	2 cut(s)	827, 860
BclI	TGATCA	3 cut(s)	27, 94, 1146
BcnI	CCSGG	2 cut(s)	458, 1318
BcoDI	GTCTC	3 cut(s)	373, 1033, 2150
BfaI	CTAG	5 cut(s)	491, 1035, 1346, 1617, 2163
BfmI	CTRYAG	3 cut(s)	654, 873, 1603
BfrI	CTTAAG	2 cut(s)	41, 1750
BfuI	GTATCC	2 cut(s)	827, 860
BglII	AGATCT	1 cut(s)	1928
BlpI	GCTNAGC	1 cut(s)	676
BmcAI	AGTACT	3 cut(s)	1021, 1372, 1699
Bme1390I	CCNGG	5 cut(s)	458, 1318, 1420, 1689, 2013
Bme18I	GGWCC	5 cut(s)	181, 1511, 1801, 1999, 2193
BmeRI	GACNNNNNGTC	1 cut(s)	1539
BmeT110I	CYCGRG	1 cut(s)	2060
BmgT120I	GGNCC	8 cut(s)	181, 1313, 1511, 1710, 1801, 1999, 2193, 2250
BmiI	GGNNCC	4 cut(s)	400, 1315, 1512, 2195
BmrFI	CCNGG	5 cut(s)	458, 1318, 1420, 1689, 2013
BmrI	ACTGGG	1 cut(s)	234
BmsI	GCATC	8 cut(s)	231, 241, 307, 668, 761, 1087, 1117, 1780
BmuI	ACTGGG	1 cut(s)	234
BpiI	GAAGAC	1 cut(s)	1410
BpmI	CTGGAG	2 cut(s)	1509, 1710
Bpu10I	CCTNAGC	1 cut(s)	1650
Bpu1102I	GCTNAGC	1 cut(s)	676
BpuEI	CTTGAG	3 cut(s)	764, 786, 1614
BpuMI	CCSGG	2 cut(s)	458, 1318
Bsa29I	ATCGAT	1 cut(s)	1593
BsaAI	YACGTR	1 cut(s)	2306
BsaI	GGTCTC	1 cut(s)	1033
BsaJI	CCNNGG	4 cut(s)	692, 1316, 1706, 1713
BsaWI	WCCGGW	1 cut(s)	955
BsaXI	ACNNNNNCTCC	2 cut(s)	186, 216
Bsc4I	CCNNNNNNNGG	8 cut(s)	1298, 1322, 1323, 1425, 1677, 1719, 1772, 2202
Bse118I	RCCGGY	2 cut(s)	1240, 1268
Bse1I	ACTGG	4 cut(s)	229, 1891, 2226, 2244
Bse3DI	GCAATG	1 cut(s)	2263
BseBI	CCWGG	3 cut(s)	1420, 1689, 2013
BseCI	ATCGAT	1 cut(s)	1593
BseDI	CCNNGG	4 cut(s)	692, 1316, 1706, 1713
BseGI	GGATG	8 cut(s)	246, 319, 442, 931, 1078, 1205, 1412, 1764
BseLI	CCNNNNNNNGG	8 cut(s)	1298, 1322, 1323, 1425, 1677, 1719, 1772, 2202
BseMI	GCAATG	1 cut(s)	2263
BseMII	CTCAG	4 cut(s)	690, 950, 1347, 1641
BseNI	ACTGG	4 cut(s)	229, 1891, 2226, 2244
BseRI	GAGGAG	1 cut(s)	2307
BseSI	GKGCMC	1 cut(s)	2143
BseXI	GCAGC	9 cut(s)	668, 809, 815, 837, 934, 1244, 1252, 1294, 1735
BseYI	CCCAGC	1 cut(s)	2252
BshFI	GGCC	4 cut(s)	1314, 1711, 2016, 2251
BshVI	ATCGAT	1 cut(s)	1593
BsiHKAI	GWGCWC	1 cut(s)	147
BsiHKCI	CYCGRG	1 cut(s)	2060
BsiSI	CCGG	6 cut(s)	457, 956, 1241, 1269, 1318, 2203
BslFI	GGGAC	2 cut(s)	1524, 2179
BslI	CCNNNNNNNGG	8 cut(s)	1298, 1322, 1323, 1425, 1677, 1719, 1772, 2202
BsmAI	GTCTC	3 cut(s)	373, 1033, 2150
BsmFI	GGGAC	2 cut(s)	1524, 2179
BsmI	GAATGC	3 cut(s)	244, 1115, 1117
BsnI	GGCC	4 cut(s)	1314, 1711, 2016, 2251
Bso31I	GGTCTC	1 cut(s)	1033
BsoBI	CYCGRG	1 cut(s)	2060
Bsp1286I	GDGCHC	2 cut(s)	147, 2143
Bsp1720I	GCTNAGC	1 cut(s)	676
BspACI	CCGC	5 cut(s)	831, 1625, 1668, 1804, 2300
BspANI	GGCC	4 cut(s)	1314, 1711, 2016, 2251
BspCNI	CTCAG	5 cut(s)	4, 689, 949, 1346, 1642
BspDI	ATCGAT	1 cut(s)	1593
BspHI	TCATGA	2 cut(s)	1132, 1870
BspLI	GGNNCC	4 cut(s)	400, 1315, 1512, 2195
BspMAI	CTGCAG	1 cut(s)	658
BspPI	GGATC	6 cut(s)	1195, 1598, 1723, 1725, 2026, 2231
BspQI	GCTCTTC	3 cut(s)	135, 1344, 2037
BspTI	CTTAAG	2 cut(s)	41, 1750
BspTNI	GGTCTC	1 cut(s)	1033
BsrDI	GCAATG	1 cut(s)	2263
BsrFI	RCCGGY	2 cut(s)	1240, 1268
BsrI	ACTGG	4 cut(s)	229, 1891, 2226, 2244
BssAI	RCCGGY	2 cut(s)	1240, 1268
BssECI	CCNNGG	4 cut(s)	692, 1316, 1706, 1713
BssT1I	CCWWGG	2 cut(s)	1706, 1713
Bst2UI	CCWGG	3 cut(s)	1420, 1689, 2013
Bst4CI	ACNGT	8 cut(s)	267, 367, 651, 1467, 1541, 1607, 1739, 2273
Bst6I	CTCTTC	5 cut(s)	135, 1221, 1344, 1670, 2037
BstAFI	CTTAAG	2 cut(s)	41, 1750
BstAPI	GCANNNNNTGC	2 cut(s)	1121, 1478
BstBAI	YACGTR	1 cut(s)	2306
BstC8I	GCNNGC	5 cut(s)	1312, 1479, 1483, 1499, 2143
BstDEI	CTNAG	6 cut(s)	12, 676, 936, 1333, 1650, 2003
BstDSI	CCRYGG	1 cut(s)	692
BstEII	GGTNACC	1 cut(s)	995
BstF5I	GGATG	8 cut(s)	246, 319, 442, 931, 1078, 1205, 1412, 1764
BstMAI	GTCTC	3 cut(s)	373, 1033, 2150
BstMWI	GCNNNNNNNGC	6 cut(s)	1121, 1478, 1622, 1631, 1782, 2212
BstNI	CCWGG	3 cut(s)	1420, 1689, 2013
BstNSI	RCATGY	4 cut(s)	190, 1501, 1850, 2171
BstPI	GGTNACC	1 cut(s)	995
BstSCI	CCNGG	5 cut(s)	456, 1316, 1418, 1687, 2011
BstSFI	CTRYAG	3 cut(s)	654, 873, 1603
BstSLI	GKGCMC	1 cut(s)	2143
BstV1I	GCAGC	9 cut(s)	668, 809, 815, 837, 934, 1244, 1252, 1294, 1735
BstV2I	GAAGAC	1 cut(s)	1410
BstX2I	RGATCY	3 cut(s)	1728, 1928, 2031
BstXI	CCANNNNNNTGG	1 cut(s)	191
BstYI	RGATCY	3 cut(s)	1728, 1928, 2031
Bsu15I	ATCGAT	1 cut(s)	1593
BsuI	GTATCC	2 cut(s)	827, 860
BsuRI	GGCC	4 cut(s)	1314, 1711, 2016, 2251
BsuTUI	ATCGAT	1 cut(s)	1593
BtgI	CCRYGG	1 cut(s)	692
BtgZI	GCGATG	1 cut(s)	653
BtsCI	GGATG	8 cut(s)	246, 319, 442, 931, 1078, 1205, 1412, 1764
BtsI	GCAGTG	2 cut(s)	162, 1402
Cac8I	GCNNGC	5 cut(s)	1312, 1479, 1483, 1499, 2143
CaiI	CAGNNNCTG	2 cut(s)	371, 662
CciI	TCATGA	2 cut(s)	1132, 1870
Cfr10I	RCCGGY	2 cut(s)	1240, 1268
Cfr13I	GGNCC	8 cut(s)	181, 1313, 1511, 1710, 1801, 1999, 2193, 2250
ClaI	ATCGAT	1 cut(s)	1593
DdeI	CTNAG	6 cut(s)	12, 676, 936, 1333, 1650, 2003
DraI	TTTAAA	1 cut(s)	1359
DriI	GACNNNNNGTC	1 cut(s)	1539
EaeI	YGGCCR	1 cut(s)	2014
Eam1104I	CTCTTC	5 cut(s)	135, 1221, 1344, 1670, 2037
Eam1105I	GACNNNNNGTC	1 cut(s)	1539
EarI	CTCTTC	5 cut(s)	135, 1221, 1344, 1670, 2037
Eco130I	CCWWGG	2 cut(s)	1706, 1713
Eco31I	GGTCTC	1 cut(s)	1033
Eco47I	GGWCC	5 cut(s)	181, 1511, 1801, 1999, 2193
Eco57I	CTGAAG	1 cut(s)	2195
Eco88I	CYCGRG	1 cut(s)	2060
Eco91I	GGTNACC	1 cut(s)	995
EcoO109I	RGGNCCY	2 cut(s)	1511, 1999
EcoO65I	GGTNACC	1 cut(s)	995
EcoRI	GAATTC	1 cut(s)	271
EcoRII	CCWGG	3 cut(s)	1418, 1687, 2011
EcoT14I	CCWWGG	2 cut(s)	1706, 1713
EcoT22I	ATGCAT	3 cut(s)	246, 1117, 1503
ErhI	CCWWGG	2 cut(s)	1706, 1713
FalI	AAGNNNNNCTT	2 cut(s)	24, 56
FaqI	GGGAC	2 cut(s)	1524, 2179
FauI	CCCGC	1 cut(s)	2307
FauNDI	CATATG	1 cut(s)	736
FbaI	TGATCA	3 cut(s)	27, 94, 1146
FblI	GTMKAC	1 cut(s)	2265
FokI	GGATG	8 cut(s)	253, 326, 449, 938, 1065, 1212, 1399, 1771
FspBI	CTAG	5 cut(s)	491, 1035, 1346, 1617, 2163
GsaI	CCCAGC	1 cut(s)	2256
GsuI	CTGGAG	2 cut(s)	1509, 1710
HaeIII	GGCC	4 cut(s)	1314, 1711, 2016, 2251
HapII	CCGG	6 cut(s)	457, 956, 1241, 1269, 1318, 2203
HincII	GTYRAC	1 cut(s)	117
HindII	GTYRAC	1 cut(s)	117
HindIII	AAGCTT	1 cut(s)	1856
HinfI	GANTC	9 cut(s)	377, 521, 716, 740, 773, 806, 1762, 1874, 2119
HpaII	CCGG	6 cut(s)	457, 956, 1241, 1269, 1318, 2203
HphI	GGTGA	6 cut(s)	17, 945, 1007, 1023, 1061, 2122
Hpy166II	GTNNAC	5 cut(s)	117, 184, 500, 2009, 2266
Hpy8I	GTNNAC	5 cut(s)	117, 184, 500, 2009, 2266
Hpy99I	CGWCG	2 cut(s)	584, 1997
HpyAV	CCTTC	3 cut(s)	191, 1639, 2098
HpyCH4III	ACNGT	8 cut(s)	267, 367, 651, 1467, 1541, 1607, 1739, 2273
HpyCH4IV	ACGT	2 cut(s)	582, 2305
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1121, 1478, 1622, 1631, 1782, 2212
HpyF3I	CTNAG	6 cut(s)	12, 676, 936, 1333, 1650, 2003
HpySE526I	ACGT	2 cut(s)	582, 2305
Ksp22I	TGATCA	3 cut(s)	27, 94, 1146
LguI	GCTCTTC	3 cut(s)	135, 1344, 2037
LmnI	GCTCC	2 cut(s)	152, 1494
Lsp1109I	GCAGC	9 cut(s)	668, 809, 815, 837, 934, 1244, 1252, 1294, 1735
LweI	GCATC	8 cut(s)	231, 241, 307, 668, 761, 1087, 1117, 1780
MaeI	CTAG	5 cut(s)	491, 1035, 1346, 1617, 2163
MaeII	ACGT	2 cut(s)	582, 2305
MaeIII	GTNAC	6 cut(s)	55, 995, 1384, 1837, 1937, 2170
MboII	GAAGA	9 cut(s)	152, 464, 711, 1238, 1361, 1415, 1687, 2054, 2201
MfeI	CAATTG	2 cut(s)	357, 1071
MflI	RGATCY	3 cut(s)	1728, 1928, 2031
MhlI	GDGCHC	2 cut(s)	147, 2143
MlsI	TGGCCA	1 cut(s)	2016
MluNI	TGGCCA	1 cut(s)	2016
MlyI	GAGTC	4 cut(s)	734, 800, 1883, 2128
MmeI	TCCRAC	3 cut(s)	405, 2264, 2268
Mox20I	TGGCCA	1 cut(s)	2016
Mph1103I	ATGCAT	3 cut(s)	246, 1117, 1503
MroXI	GAANNNNTTC	1 cut(s)	1115
MscI	TGGCCA	1 cut(s)	2016
MseI	TTAA	7 cut(s)	42, 554, 882, 1044, 1358, 1751, 1860
MslI	CAYNNNNRTG	2 cut(s)	249, 505
Msp20I	TGGCCA	1 cut(s)	2016
MspA1I	CMGCKG	2 cut(s)	680, 828
MspCI	CTTAAG	2 cut(s)	41, 1750
MspI	CCGG	6 cut(s)	457, 956, 1241, 1269, 1318, 2203
MspR9I	CCNGG	5 cut(s)	458, 1318, 1420, 1689, 2013
MunI	CAATTG	2 cut(s)	357, 1071
Mva1269I	GAATGC	3 cut(s)	244, 1115, 1117
MvaI	CCWGG	3 cut(s)	1420, 1689, 2013
MwoI	GCNNNNNNNGC	6 cut(s)	1121, 1478, 1622, 1631, 1782, 2212
NciI	CCSGG	2 cut(s)	458, 1318
NdeI	CATATG	1 cut(s)	736
NlaIV	GGNNCC	4 cut(s)	400, 1315, 1512, 2195
NmuCI	GTSAC	3 cut(s)	995, 1384, 1837
NsiI	ATGCAT	3 cut(s)	246, 1117, 1503
NspI	RCATGY	4 cut(s)	190, 1501, 1850, 2171
PaeI	GCATGC	1 cut(s)	1501
PaeR7I	CTCGAG	1 cut(s)	2060
PagI	TCATGA	2 cut(s)	1132, 1870
PciSI	GCTCTTC	3 cut(s)	135, 1344, 2037
PctI	GAATGC	3 cut(s)	244, 1115, 1117
PdmI	GAANNNNTTC	1 cut(s)	1115
PfeI	GAWTC	5 cut(s)	377, 521, 716, 773, 1762
PflMI	CCANNNNNTGG	1 cut(s)	1298
PfoI	TCCNGGA	1 cut(s)	456
PleI	GAGTC	4 cut(s)	734, 800, 1882, 2127
PpsI	GAGTC	4 cut(s)	734, 800, 1882, 2127
Ppu21I	YACGTR	1 cut(s)	2306
PpuMI	RGGWCCY	2 cut(s)	1511, 1999
Psp5II	RGGWCCY	2 cut(s)	1511, 1999
Psp6I	CCWGG	3 cut(s)	1418, 1687, 2011
PspEI	GGTNACC	1 cut(s)	995
PspFI	CCCAGC	1 cut(s)	2252
PspGI	CCWGG	3 cut(s)	1418, 1687, 2011
PspN4I	GGNNCC	4 cut(s)	400, 1315, 1512, 2195
PspPI	GGNCC	8 cut(s)	181, 1313, 1511, 1710, 1801, 1999, 2193, 2250
PspPPI	RGGWCCY	2 cut(s)	1511, 1999
PstI	CTGCAG	1 cut(s)	658
PstNI	CAGNNNCTG	2 cut(s)	371, 662
PsuI	RGATCY	3 cut(s)	1728, 1928, 2031
PvuII	CAGCTG	2 cut(s)	680, 828
RseI	CAYNNNNRTG	2 cut(s)	249, 505
SapI	GCTCTTC	3 cut(s)	135, 1344, 2037
SaqAI	TTAA	7 cut(s)	42, 554, 882, 1044, 1358, 1751, 1860
Sau96I	GGNCC	8 cut(s)	181, 1313, 1511, 1710, 1801, 1999, 2193, 2250
ScaI	AGTACT	3 cut(s)	1021, 1372, 1699
SchI	GAGTC	4 cut(s)	734, 800, 1883, 2128
ScrFI	CCNGG	5 cut(s)	458, 1318, 1420, 1689, 2013
SduI	GDGCHC	2 cut(s)	147, 2143
SfaNI	GCATC	8 cut(s)	231, 241, 307, 668, 761, 1087, 1117, 1780
SfcI	CTRYAG	3 cut(s)	654, 873, 1603
Sfr274I	CTCGAG	1 cut(s)	2060
SinI	GGWCC	5 cut(s)	181, 1511, 1801, 1999, 2193
SlaI	CTCGAG	1 cut(s)	2060
SmiMI	CAYNNNNRTG	2 cut(s)	249, 505
SmlI	CTYRAG	6 cut(s)	41, 779, 801, 1629, 1750, 2060
SmoI	CTYRAG	6 cut(s)	41, 779, 801, 1629, 1750, 2060
SphI	GCATGC	1 cut(s)	1501
SsiI	CCGC	5 cut(s)	831, 1625, 1668, 1804, 2300
SspMI	CTAG	5 cut(s)	491, 1035, 1346, 1617, 2163
StyD4I	CCNGG	5 cut(s)	456, 1316, 1418, 1687, 2011
StyI	CCWWGG	2 cut(s)	1706, 1713
TaaI	ACNGT	8 cut(s)	267, 367, 651, 1467, 1541, 1607, 1739, 2273
TaiI	ACGT	2 cut(s)	585, 2308
TaqI	TCGA	7 cut(s)	288, 298, 608, 1593, 1702, 1995, 2061
TaqII	GACCGA	1 cut(s)	1015
TatI	WGTACW	7 cut(s)	497, 701, 1019, 1370, 1697, 1815, 2006
TauI	GCSGC	1 cut(s)	1628
TfiI	GAWTC	5 cut(s)	377, 521, 716, 773, 1762
Tru1I	TTAA	7 cut(s)	42, 554, 882, 1044, 1358, 1751, 1860
Tru9I	TTAA	7 cut(s)	42, 554, 882, 1044, 1358, 1751, 1860
TseFI	GTSAC	3 cut(s)	995, 1384, 1837
TseI	GCWGC	9 cut(s)	656, 797, 825, 828, 947, 1257, 1265, 1307, 1723
Tsp45I	GTSAC	3 cut(s)	995, 1384, 1837
Van91I	CCANNNNNTGG	1 cut(s)	1298
Vha464I	CTTAAG	2 cut(s)	41, 1750
VpaK11BI	GGWCC	5 cut(s)	181, 1511, 1801, 1999, 2193
XapI	RAATTY	3 cut(s)	103, 271, 643
XceI	RCATGY	4 cut(s)	190, 1501, 1850, 2171
XcmI	CCANNNNNNNNNTGG	2 cut(s)	341, 919
XhoI	CTCGAG	1 cut(s)	2060
XmiI	GTMKAC	1 cut(s)	2265
XmnI	GAANNNNTTC	1 cut(s)	1115
XspI	CTAG	5 cut(s)	491, 1035, 1346, 1617, 2163
ZrmI	AGTACT	3 cut(s)	1021, 1372, 1699
Zsp2I	ATGCAT	3 cut(s)	246, 1117, 1503

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.