Rorug06G0475000
BHLH Family

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000912
Physical Location & Seq
Reverse (-)
59750672 .. 59752698
2027 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0475000.1

Sequence Viewer

Length: 714 bp
ATGGAGAAAGAGGTGAACGAAAGTCGAAAGGGCCGAAAGGCTGGTGGTGGTGCGTGGGTTGAGAGAGAGCGAAGGAAGAAGATGAAGGATATGTTCTCTTCCCTTCATGCCTTGCTTCCTCAGCTTCCAGCTAAGGCTGATCATTGTACCATTGTTGATGAGGCAGTGAGATATATCAAATCCCTCGAGCACACTCTACAAACAGCGCAAAAGCAAAGGCTTGCTAAATTGCGGAGTAGCGCTTCTTCATCCATTATTACATCGAAAACAGAAGCTAGGAGTGATGCTGGTGTTACAAGAAAAGCGTTTTTAGCTGATCATTTTCAGGAGGAGGAGGAGGATCGACCATCAAAGAAGTTGGCTTACGGAAACAATCCATCACCTACTTCTCTTGATTCTCAGCAGGCCAGCTGCTTCAAGACGTGGTTTTCCCCTAATATTGTGGTGAACATATGCGGCAATGAGGCTCACGTAAGTGTTTGTTCGCCAAGAAGGCCAGGGCTCCTCAGTACCGTCTTCTACATACTAGAAAAGCATAAACTGGATGTTATATCTGCTCACATTTCCTCGGATCAGCATCGTTGTATGTACATGATCCACGCTCATTATGCTGGTGGAGCTTGTGATGATACTCTTCCAGCTGGGGCGTTATCAGTAGAGGACACTTTCAATCTAGCAGCAGGTGAACTGAACCTGTGCCTCTTTTCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

237

Amino Acids

26.22

Weight (kDa)

8.43

Isoelectric Point (pI)

64.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 20 - 63 1.5e-09 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 147 - 199 3e-07 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 671
Acc36I ACCTGC 1 cut(s) 671
AciI CCGC 2 cut(s) 232, 456
AclWI GGATC 3 cut(s) 348, 579, 589
AfaI GTAC 3 cut(s) 148, 511, 590
AfeI AGCGCT 1 cut(s) 241
AgsI TTSAA 2 cut(s) 418, 670
AjiI CACGTC 1 cut(s) 423
AjnI CCWGG 1 cut(s) 496
AleI CACNNNNGTG 1 cut(s) 474
AluBI AGCT 7 cut(s) 124, 131, 275, 314, 411, 620, 641
AluI AGCT 7 cut(s) 124, 131, 275, 314, 411, 620, 641
Alw21I GWGCWC 1 cut(s) 192
AlwI GGATC 3 cut(s) 348, 579, 589
Ama87I CYCGRG 1 cut(s) 185
Aor51HI AGCGCT 1 cut(s) 241
AoxI GGCC 3 cut(s) 31, 405, 494
ApeKI GCWGC 2 cut(s) 411, 677
AspLEI GCGC 2 cut(s) 208, 242
AspS9I GGNCC 1 cut(s) 31
AsuHPI GGTGA 4 cut(s) 25, 372, 457, 695
AvaI CYCGRG 1 cut(s) 185
BanII GRGCYC 1 cut(s) 504
BarI GAAGNNNNNNTAC 4 cut(s) 229, 261, 347, 379
BbsI GAAGAC 1 cut(s) 508
Bbv12I GWGCWC 1 cut(s) 192
BbvCI CCTCAGC 1 cut(s) 120
BbvI GCAGC 2 cut(s) 398, 689
BccI CCATC 2 cut(s) 355, 385
BciT130I CCWGG 1 cut(s) 498
BclI TGATCA 2 cut(s) 139, 316
BfaI CTAG 3 cut(s) 276, 527, 674
BfoI RGCGCY 1 cut(s) 243
BfuAI ACCTGC 1 cut(s) 671
BglI GCCNNNNNGGC 1 cut(s) 493
BisI GCNGC 3 cut(s) 412, 457, 678
BlsI GCNGC 3 cut(s) 413, 458, 679
Bme1390I CCNGG 1 cut(s) 498
BmeT110I CYCGRG 1 cut(s) 185
BmgBI CACGTC 1 cut(s) 423
BmgT120I GGNCC 1 cut(s) 31
BmiI GGNNCC 1 cut(s) 503
BmrFI CCNGG 1 cut(s) 498
BmsI GCATC 2 cut(s) 274, 586
BpiI GAAGAC 1 cut(s) 508
Bpu10I CCTNAGC 2 cut(s) 120, 132
BsaAI YACGTR 1 cut(s) 472
BsaBI GATNNNNATC 1 cut(s) 576
BsaJI CCNNGG 2 cut(s) 497, 567
Bse1I ACTGG 1 cut(s) 546
Bse3DI GCAATG 1 cut(s) 466
Bse8I GATNNNNATC 1 cut(s) 576
BseBI CCWGG 1 cut(s) 498
BseDI CCNNGG 2 cut(s) 497, 567
BseGI GGATG 2 cut(s) 248, 550
BseJI GATNNNNATC 1 cut(s) 576
BseMI GCAATG 1 cut(s) 466
BseMII CTCAG 3 cut(s) 134, 413, 520
BseNI ACTGG 1 cut(s) 546
BseRI GAGGAG 4 cut(s) 344, 347, 350, 494
BseXI GCAGC 2 cut(s) 398, 689
BseYI CCCAGC 1 cut(s) 641
BshFI GGCC 3 cut(s) 33, 407, 496
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 185
BsnI GGCC 3 cut(s) 33, 407, 496
BsoBI CYCGRG 1 cut(s) 185
Bsp1286I GDGCHC 2 cut(s) 192, 504
Bsp1407I TGTACA 1 cut(s) 588
Bsp143I GATC 5 cut(s) 139, 316, 340, 571, 594
BspACI CCGC 2 cut(s) 232, 456
BspANI GGCC 3 cut(s) 33, 407, 496
BspCNI CTCAG 3 cut(s) 133, 412, 519
BspLI GGNNCC 1 cut(s) 503
BspMI ACCTGC 1 cut(s) 671
BspPI GGATC 3 cut(s) 348, 579, 589
BsrDI GCAATG 1 cut(s) 466
BsrGI TGTACA 1 cut(s) 588
BsrI ACTGG 1 cut(s) 546
BssECI CCNNGG 2 cut(s) 497, 567
BssMI GATC 5 cut(s) 139, 316, 340, 571, 594
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 1 cut(s) 514
Bst6I CTCTTC 2 cut(s) 103, 639
BstAUI TGTACA 1 cut(s) 588
BstBAI YACGTR 1 cut(s) 472
BstC8I GCNNGC 3 cut(s) 222, 405, 409
BstDEI CTNAG 4 cut(s) 120, 132, 399, 506
BstF5I GGATG 2 cut(s) 248, 550
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 2 cut(s) 208, 242
BstKTI GATC 5 cut(s) 142, 319, 343, 574, 597
BstMBI GATC 5 cut(s) 139, 316, 340, 571, 594
BstMWI GCNNNNNNNGC 5 cut(s) 121, 311, 493, 608, 617
BstNI CCWGG 1 cut(s) 498
BstSCI CCNGG 1 cut(s) 496
BstV1I GCAGC 2 cut(s) 398, 689
BstV2I GAAGAC 1 cut(s) 508
BsuRI GGCC 3 cut(s) 33, 407, 496
BtrI CACGTC 1 cut(s) 423
BtsCI GGATG 2 cut(s) 248, 550
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 1 cut(s) 171
BveI ACCTGC 1 cut(s) 671
Cac8I GCNNGC 3 cut(s) 222, 405, 409
CfoI GCGC 2 cut(s) 208, 242
Cfr13I GGNCC 1 cut(s) 31
Csp6I GTAC 3 cut(s) 147, 510, 589
CviAII CATG 2 cut(s) 107, 592
CviQI GTAC 3 cut(s) 147, 510, 589
DdeI CTNAG 4 cut(s) 120, 132, 399, 506
DpnI GATC 5 cut(s) 141, 318, 342, 573, 596
DpnII GATC 5 cut(s) 139, 316, 340, 571, 594
Eam1104I CTCTTC 2 cut(s) 103, 639
EarI CTCTTC 2 cut(s) 103, 639
Eco24I GRGCYC 1 cut(s) 504
Eco47III AGCGCT 1 cut(s) 241
Eco88I CYCGRG 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 496
EcoT38I GRGCYC 1 cut(s) 504
FaeI CATG 2 cut(s) 110, 595
FatI CATG 2 cut(s) 106, 591
FauNDI CATATG 1 cut(s) 452
FbaI TGATCA 2 cut(s) 139, 316
Fnu4HI GCNGC 3 cut(s) 412, 457, 678
FokI GGATG 2 cut(s) 235, 557
FriOI GRGCYC 1 cut(s) 504
Fsp4HI GCNGC 3 cut(s) 412, 457, 678
FspBI CTAG 3 cut(s) 276, 527, 674
GlaI GCGC 2 cut(s) 207, 241
GluI GCNGC 3 cut(s) 412, 457, 678
GsaI CCCAGC 1 cut(s) 645
HaeII RGCGCY 1 cut(s) 243
HaeIII GGCC 3 cut(s) 33, 407, 496
HhaI GCGC 2 cut(s) 208, 242
Hin1II CATG 2 cut(s) 110, 595
Hin6I GCGC 2 cut(s) 206, 240
HinP1I GCGC 2 cut(s) 206, 240
HinfI GANTC 1 cut(s) 395
HphI GGTGA 4 cut(s) 25, 372, 457, 695
Hpy166II GTNNAC 3 cut(s) 16, 448, 686
Hpy188I TCNGA 1 cut(s) 571
Hpy188III TCNNGA 4 cut(s) 326, 392, 418, 711
Hpy8I GTNNAC 3 cut(s) 16, 448, 686
HpyAV CCTTC 4 cut(s) 66, 79, 113, 486
HpyCH4III ACNGT 1 cut(s) 514
HpyCH4IV ACGT 2 cut(s) 422, 471
HpyF10VI GCNNNNNNNGC 5 cut(s) 121, 311, 493, 608, 617
HpyF3I CTNAG 4 cut(s) 120, 132, 399, 506
HpySE526I ACGT 2 cut(s) 422, 471
Hsp92II CATG 2 cut(s) 110, 595
HspAI GCGC 2 cut(s) 206, 240
Ksp22I TGATCA 2 cut(s) 139, 316
Kzo9I GATC 5 cut(s) 139, 316, 340, 571, 594
LmnI GCTCC 2 cut(s) 507, 617
Lsp1109I GCAGC 2 cut(s) 398, 689
LweI GCATC 2 cut(s) 274, 586
MaeI CTAG 3 cut(s) 276, 527, 674
MaeII ACGT 2 cut(s) 422, 471
MaeIII GTNAC 1 cut(s) 292
MalI GATC 5 cut(s) 141, 318, 342, 573, 596
MboI GATC 5 cut(s) 139, 316, 340, 571, 594
MboII GAAGA 6 cut(s) 88, 90, 91, 237, 508, 626
MhlI GDGCHC 2 cut(s) 192, 504
MluCI AATT 1 cut(s) 227
MslI CAYNNNNRTG 1 cut(s) 474
MspA1I CMGCKG 2 cut(s) 411, 641
MspR9I CCNGG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 498
MwoI GCNNNNNNNGC 5 cut(s) 121, 311, 493, 608, 617
NdeI CATATG 1 cut(s) 452
NdeII GATC 5 cut(s) 139, 316, 340, 571, 594
NlaIII CATG 2 cut(s) 110, 595
NlaIV GGNNCC 1 cut(s) 503
OliI CACNNNNGTG 1 cut(s) 474
PaeR7I CTCGAG 1 cut(s) 185
PaqCI CACCTGC 1 cut(s) 671
PcsI WCGNNNNNNNCGW 1 cut(s) 31
PfeI GAWTC 1 cut(s) 395
PkrI GCNGC 3 cut(s) 413, 458, 679
Ppu21I YACGTR 1 cut(s) 472
Psp6I CCWGG 1 cut(s) 496
PspFI CCCAGC 1 cut(s) 641
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 1 cut(s) 503
PspPI GGNCC 1 cut(s) 31
PspXI VCTCGAGB 1 cut(s) 185
PvuII CAGCTG 2 cut(s) 411, 641
RsaI GTAC 3 cut(s) 148, 511, 590
RsaNI GTAC 3 cut(s) 147, 510, 589
RseI CAYNNNNRTG 1 cut(s) 474
SatI GCNGC 3 cut(s) 412, 457, 678
Sau3AI GATC 5 cut(s) 139, 316, 340, 571, 594
Sau96I GGNCC 1 cut(s) 31
ScrFI CCNGG 1 cut(s) 498
SduI GDGCHC 2 cut(s) 192, 504
SfaNI GCATC 2 cut(s) 274, 586
Sfr274I CTCGAG 1 cut(s) 185
SlaI CTCGAG 1 cut(s) 185
SmiMI CAYNNNNRTG 1 cut(s) 474
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 1 cut(s) 227
SsiI CCGC 2 cut(s) 232, 456
SspI AATATT 1 cut(s) 439
SspMI CTAG 3 cut(s) 276, 527, 674
StyD4I CCNGG 1 cut(s) 496
TaaI ACNGT 1 cut(s) 514
TaiI ACGT 2 cut(s) 425, 474
TaqI TCGA 4 cut(s) 25, 186, 263, 343
TasI AATT 1 cut(s) 227
TatI WGTACW 1 cut(s) 588
TauI GCSGC 1 cut(s) 459
TfiI GAWTC 1 cut(s) 395
TscAI CASTG 1 cut(s) 171
TseI GCWGC 2 cut(s) 411, 677
TspDTI ATGAA 4 cut(s) 95, 98, 237, 696
TspGWI ACGGA 1 cut(s) 381
TspRI CASTG 1 cut(s) 171
XhoI CTCGAG 1 cut(s) 185
XspI CTAG 3 cut(s) 276, 527, 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.