Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 259 |
| AccB1I | GGYRCC | 1 cut(s) | 400 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1721 |
| AccI | GTMKAC | 1 cut(s) | 3048 |
| AccIII | TCCGGA | 1 cut(s) | 244 |
| AclWI | GGATC | 2 cut(s) | 1579, 2826 |
| AcoI | YGGCCR | 5 cut(s) | 274, 1285, 1605, 1723, 3036 |
| AcsI | RAATTY | 5 cut(s) | 406, 1830, 2027, 3061, 3068 |
| AfaI | GTAC | 8 cut(s) | 14, 527, 742, 1067, 1455, 2149, 2165, 2761 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 272, 1721, 1732, 1860, 2123 |
| AjnI | CCWGG | 1 cut(s) | 3038 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1158, 1190, 2607, 2639 |
| Alw21I | GWGCWC | 2 cut(s) | 8, 1873 |
| Alw26I | GTCTC | 5 cut(s) | 68, 213, 267, 974, 2283 |
| AlwI | GGATC | 2 cut(s) | 1579, 2826 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 647, 1478 |
| Ama87I | CYCGRG | 1 cut(s) | 1685 |
| Aor13HI | TCCGGA | 1 cut(s) | 244 |
| ApeKI | GCWGC | 6 cut(s) | 132, 719, 1234, 1315, 2683, 3023 |
| ApoI | RAATTY | 5 cut(s) | 406, 1830, 2027, 3061, 3068 |
| AseI | ATTAAT | 1 cut(s) | 462 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 547, 2031, 2454 |
| AspLEI | GCGC | 2 cut(s) | 191, 3023 |
| AspS9I | GGNCC | 3 cut(s) | 198, 912, 2361 |
| AsuHPI | GGTGA | 6 cut(s) | 753, 1118, 1663, 2138, 2202, 2567 |
| AvaI | CYCGRG | 1 cut(s) | 1685 |
| BaeGI | GKGCMC | 1 cut(s) | 2143 |
| BalI | TGGCCA | 2 cut(s) | 1607, 3038 |
| BanI | GGYRCC | 1 cut(s) | 400 |
| BanII | GRGCYC | 2 cut(s) | 8, 2886 |
| BauI | CACGAG | 1 cut(s) | 2280 |
| BbsI | GAAGAC | 4 cut(s) | 500, 1110, 1598, 2559 |
| Bbv12I | GWGCWC | 2 cut(s) | 8, 1873 |
| BbvI | GCAGC | 6 cut(s) | 119, 706, 1246, 1302, 2695, 3035 |
| BccI | CCATC | 7 cut(s) | 32, 125, 443, 1651, 1715, 1892, 3037 |
| BceAI | ACGGC | 3 cut(s) | 270, 289, 771 |
| BciT130I | CCWGG | 1 cut(s) | 3040 |
| BciVI | GTATCC | 1 cut(s) | 1073 |
| BcoDI | GTCTC | 5 cut(s) | 68, 213, 267, 974, 2283 |
| BfaI | CTAG | 2 cut(s) | 1185, 1922 |
| BfmI | CTRYAG | 3 cut(s) | 864, 1303, 1479 |
| BfuI | GTATCC | 1 cut(s) | 1073 |
| BglII | AGATCT | 1 cut(s) | 2387 |
| BisI | GCNGC | 9 cut(s) | 133, 201, 204, 207, 720, 1235, 1316, 2684, 3024 |
| BlsI | GCNGC | 9 cut(s) | 134, 202, 205, 208, 721, 1236, 1317, 2685, 3025 |
| BmcAI | AGTACT | 2 cut(s) | 527, 2165 |
| Bme1390I | CCNGG | 1 cut(s) | 3040 |
| BmeT110I | CYCGRG | 1 cut(s) | 1685 |
| BmgT120I | GGNCC | 3 cut(s) | 198, 912, 2361 |
| BmiI | GGNNCC | 4 cut(s) | 306, 402, 1755, 2363 |
| BmrFI | CCNGG | 1 cut(s) | 3040 |
| BmsI | GCATC | 5 cut(s) | 250, 457, 1917, 1982, 2073 |
| BpiI | GAAGAC | 4 cut(s) | 500, 1110, 1598, 2559 |
| Bpu10I | CCTNAGC | 3 cut(s) | 567, 2157, 2880 |
| BpuEI | CTTGAG | 2 cut(s) | 596, 816 |
| BsaAI | YACGTR | 1 cut(s) | 740 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1368, 1996 |
| BsaI | GGTCTC | 1 cut(s) | 68 |
| BsaJI | CCNNGG | 4 cut(s) | 2052, 2151, 2547, 2928 |
| BsaWI | WCCGGW | 2 cut(s) | 244, 2469 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2857, 2887 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 272, 1721, 1732, 1860, 2123 |
| Bse118I | RCCGGY | 2 cut(s) | 276, 1725 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1368, 1996 |
| BseAI | TCCGGA | 1 cut(s) | 244 |
| BseBI | CCWGG | 1 cut(s) | 3040 |
| BseDI | CCNNGG | 4 cut(s) | 2052, 2151, 2547, 2928 |
| BseGI | GGATG | 7 cut(s) | 24, 151, 241, 553, 1726, 2048, 3048 |
| BseJI | GATNNNNATC | 2 cut(s) | 1368, 1996 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 272, 1721, 1732, 1860, 2123 |
| BseRI | GAGGAG | 2 cut(s) | 81, 2878 |
| BseSI | GKGCMC | 1 cut(s) | 2143 |
| BseX3I | CGGCCG | 1 cut(s) | 274 |
| BseXI | GCAGC | 6 cut(s) | 119, 706, 1246, 1302, 2695, 3035 |
| Bsh1285I | CGRYCG | 1 cut(s) | 277 |
| BshNI | GGYRCC | 1 cut(s) | 400 |
| BsiEI | CGRYCG | 1 cut(s) | 277 |
| BsiHKAI | GWGCWC | 2 cut(s) | 8, 1873 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1685 |
| BsiSI | CCGG | 5 cut(s) | 228, 245, 277, 1726, 2470 |
| BslFI | GGGAC | 2 cut(s) | 2525, 2702 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 272, 1721, 1732, 1860, 2123 |
| BsmAI | GTCTC | 5 cut(s) | 68, 213, 267, 974, 2283 |
| BsmFI | GGGAC | 2 cut(s) | 2525, 2702 |
| BsmI | GAATGC | 1 cut(s) | 2737 |
| Bso31I | GGTCTC | 1 cut(s) | 68 |
| BsoBI | CYCGRG | 1 cut(s) | 1685 |
| Bsp1286I | GDGCHC | 4 cut(s) | 8, 1873, 2143, 2886 |
| Bsp13I | TCCGGA | 1 cut(s) | 244 |
| Bsp143I | GATC | 6 cut(s) | 975, 1038, 1571, 1803, 2387, 2818 |
| Bsp19I | CCATGG | 1 cut(s) | 2151 |
| BspEI | TCCGGA | 1 cut(s) | 244 |
| BspHI | TCATGA | 3 cut(s) | 51, 2332, 2434 |
| BspLI | GGNNCC | 4 cut(s) | 306, 402, 1755, 2363 |
| BspMAI | CTGCAG | 1 cut(s) | 1483 |
| BspPI | GGATC | 2 cut(s) | 1579, 2826 |
| BspT107I | GGYRCC | 1 cut(s) | 400 |
| BspTNI | GGTCTC | 1 cut(s) | 68 |
| BsrFI | RCCGGY | 2 cut(s) | 276, 1725 |
| BssAI | RCCGGY | 2 cut(s) | 276, 1725 |
| BssECI | CCNNGG | 4 cut(s) | 2052, 2151, 2547, 2928 |
| BssMI | GATC | 6 cut(s) | 975, 1038, 1571, 1803, 2387, 2818 |
| BssSI | CACGAG | 1 cut(s) | 2280 |
| BssT1I | CCWWGG | 3 cut(s) | 2151, 2547, 2928 |
| Bst2BI | CACGAG | 1 cut(s) | 2280 |
| Bst2UI | CCWGG | 1 cut(s) | 3040 |
| Bst6I | CTCTTC | 4 cut(s) | 73, 921, 1299, 2748 |
| BstBAI | YACGTR | 1 cut(s) | 740 |
| BstC8I | GCNNGC | 9 cut(s) | 595, 717, 855, 1313, 1389, 1704, 2044, 2205, 2800 |
| BstDSI | CCRYGG | 1 cut(s) | 2151 |
| BstEII | GGTNACC | 1 cut(s) | 1849 |
| BstF5I | GGATG | 7 cut(s) | 24, 151, 241, 553, 1726, 2048, 3048 |
| BstHHI | GCGC | 2 cut(s) | 191, 3023 |
| BstKTI | GATC | 6 cut(s) | 978, 1041, 1574, 1806, 2390, 2821 |
| BstMAI | GTCTC | 5 cut(s) | 68, 213, 267, 974, 2283 |
| BstMBI | GATC | 6 cut(s) | 975, 1038, 1571, 1803, 2387, 2818 |
| BstMCI | CGRYCG | 1 cut(s) | 277 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 167, 197, 206, 282, 794, 1613, 2243 |
| BstNI | CCWGG | 1 cut(s) | 3040 |
| BstNSI | RCATGY | 3 cut(s) | 719, 2046, 2078 |
| BstPI | GGTNACC | 1 cut(s) | 1849 |
| BstSCI | CCNGG | 1 cut(s) | 3038 |
| BstSFI | CTRYAG | 3 cut(s) | 864, 1303, 1479 |
| BstSLI | GKGCMC | 1 cut(s) | 2143 |
| BstV1I | GCAGC | 6 cut(s) | 119, 706, 1246, 1302, 2695, 3035 |
| BstV2I | GAAGAC | 4 cut(s) | 500, 1110, 1598, 2559 |
| BstX2I | RGATCY | 2 cut(s) | 1571, 2387 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2959 |
| BstYI | RGATCY | 2 cut(s) | 1571, 2387 |
| BstZI | CGGCCG | 1 cut(s) | 274 |
| BsuI | GTATCC | 1 cut(s) | 1073 |
| BtgI | CCRYGG | 1 cut(s) | 2151 |
| BtsCI | GGATG | 7 cut(s) | 24, 151, 241, 553, 1726, 2048, 3048 |
| BtsIMutI | CAGTG | 4 cut(s) | 418, 661, 2617, 2828 |
| Cac8I | GCNNGC | 9 cut(s) | 595, 717, 855, 1313, 1389, 1704, 2044, 2205, 2800 |
| CaiI | CAGNNNCTG | 2 cut(s) | 647, 1478 |
| CciI | TCATGA | 3 cut(s) | 51, 2332, 2434 |
| CfoI | GCGC | 2 cut(s) | 191, 3023 |
| Cfr10I | RCCGGY | 2 cut(s) | 276, 1725 |
| Cfr13I | GGNCC | 3 cut(s) | 198, 912, 2361 |
| CseI | GACGC | 1 cut(s) | 1925 |
| Csp6I | GTAC | 8 cut(s) | 13, 526, 741, 1066, 1454, 2148, 2164, 2760 |
| CviQI | GTAC | 8 cut(s) | 13, 526, 741, 1066, 1454, 2148, 2164, 2760 |
| DpnI | GATC | 6 cut(s) | 977, 1040, 1573, 1805, 2389, 2820 |
| DpnII | GATC | 6 cut(s) | 975, 1038, 1571, 1803, 2387, 2818 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 259 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 259 |
| EaeI | YGGCCR | 5 cut(s) | 274, 1285, 1605, 1723, 3036 |
| EagI | CGGCCG | 1 cut(s) | 274 |
| Eam1104I | CTCTTC | 4 cut(s) | 73, 921, 1299, 2748 |
| EarI | CTCTTC | 4 cut(s) | 73, 921, 1299, 2748 |
| EciI | GGCGGA | 1 cut(s) | 2462 |
| Ecl136II | GAGCTC | 1 cut(s) | 6 |
| EclXI | CGGCCG | 1 cut(s) | 274 |
| Eco130I | CCWWGG | 3 cut(s) | 2151, 2547, 2928 |
| Eco24I | GRGCYC | 2 cut(s) | 8, 2886 |
| Eco31I | GGTCTC | 1 cut(s) | 68 |
| Eco32I | GATATC | 3 cut(s) | 235, 610, 2775 |
| Eco52I | CGGCCG | 1 cut(s) | 274 |
| Eco53kI | GAGCTC | 1 cut(s) | 6 |
| Eco88I | CYCGRG | 1 cut(s) | 1685 |
| Eco91I | GGTNACC | 1 cut(s) | 1849 |
| EcoICRI | GAGCTC | 1 cut(s) | 6 |
| EcoO65I | GGTNACC | 1 cut(s) | 1849 |
| EcoRI | GAATTC | 1 cut(s) | 3061 |
| EcoRII | CCWGG | 1 cut(s) | 3038 |
| EcoRV | GATATC | 3 cut(s) | 235, 610, 2775 |
| EcoT14I | CCWWGG | 3 cut(s) | 2151, 2547, 2928 |
| EcoT22I | ATGCAT | 1 cut(s) | 2080 |
| EcoT38I | GRGCYC | 2 cut(s) | 8, 2886 |
| ErhI | CCWWGG | 3 cut(s) | 2151, 2547, 2928 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1158, 1190, 2607, 2639 |
| FaqI | GGGAC | 2 cut(s) | 2525, 2702 |
| FauI | CCCGC | 3 cut(s) | 80, 1311, 2679 |
| FauNDI | CATATG | 3 cut(s) | 885, 964, 1438 |
| FblI | GTMKAC | 1 cut(s) | 3048 |
| Fnu4HI | GCNGC | 9 cut(s) | 133, 201, 204, 207, 720, 1235, 1316, 2684, 3024 |
| FokI | GGATG | 7 cut(s) | 11, 138, 228, 540, 1733, 2035, 3055 |
| FriOI | GRGCYC | 2 cut(s) | 8, 2886 |
| Fsp4HI | GCNGC | 9 cut(s) | 133, 201, 204, 207, 720, 1235, 1316, 2684, 3024 |
| FspBI | CTAG | 2 cut(s) | 1185, 1922 |
| GlaI | GCGC | 2 cut(s) | 190, 3022 |
| GluI | GCNGC | 9 cut(s) | 133, 201, 204, 207, 720, 1235, 1316, 2684, 3024 |
| HapII | CCGG | 5 cut(s) | 228, 245, 277, 1726, 2470 |
| HgaI | GACGC | 1 cut(s) | 1925 |
| HhaI | GCGC | 2 cut(s) | 191, 3023 |
| Hin6I | GCGC | 2 cut(s) | 189, 3021 |
| HinP1I | GCGC | 2 cut(s) | 189, 3021 |
| HincII | GTYRAC | 3 cut(s) | 639, 652, 2590 |
| HindII | GTYRAC | 3 cut(s) | 639, 652, 2590 |
| HindIII | AAGCTT | 4 cut(s) | 371, 851, 1331, 2796 |
| HinfI | GANTC | 8 cut(s) | 48, 644, 685, 1023, 1250, 1281, 1369, 2699 |
| HpaII | CCGG | 5 cut(s) | 228, 245, 277, 1726, 2470 |
| HphI | GGTGA | 6 cut(s) | 753, 1118, 1663, 2138, 2202, 2567 |
| HpyCH4IV | ACGT | 4 cut(s) | 739, 1228, 1959, 2677 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 167, 197, 206, 282, 794, 1613, 2243 |
| HpySE526I | ACGT | 4 cut(s) | 739, 1228, 1959, 2677 |
| HspAI | GCGC | 2 cut(s) | 189, 3021 |
| Kpn2I | TCCGGA | 1 cut(s) | 244 |
| Kzo9I | GATC | 6 cut(s) | 975, 1038, 1571, 1803, 2387, 2818 |
| LmnI | GCTCC | 4 cut(s) | 11, 310, 1759, 2093 |
| Lsp1109I | GCAGC | 6 cut(s) | 119, 706, 1246, 1302, 2695, 3035 |
| LweI | GCATC | 5 cut(s) | 250, 457, 1917, 1982, 2073 |
| MaeI | CTAG | 2 cut(s) | 1185, 1922 |
| MaeII | ACGT | 4 cut(s) | 739, 1228, 1959, 2677 |
| MaeIII | GTNAC | 9 cut(s) | 698, 934, 967, 1238, 1474, 1849, 1945, 2416, 2923 |
| MalI | GATC | 6 cut(s) | 977, 1040, 1573, 1805, 2389, 2820 |
| MboI | GATC | 6 cut(s) | 975, 1038, 1571, 1803, 2387, 2818 |
| MflI | RGATCY | 2 cut(s) | 1571, 2387 |
| MhlI | GDGCHC | 4 cut(s) | 8, 1873, 2143, 2886 |
| MlsI | TGGCCA | 2 cut(s) | 1607, 3038 |
| MluNI | TGGCCA | 2 cut(s) | 1607, 3038 |
| MlyI | GAGTC | 2 cut(s) | 653, 2693 |
| MmeI | TCCRAC | 5 cut(s) | 249, 1258, 1549, 2296, 2940 |
| Mox20I | TGGCCA | 2 cut(s) | 1607, 3038 |
| Mph1103I | ATGCAT | 1 cut(s) | 2080 |
| MroI | TCCGGA | 1 cut(s) | 244 |
| MroXI | GAANNNNTTC | 3 cut(s) | 547, 2031, 2454 |
| MscI | TGGCCA | 2 cut(s) | 1607, 3038 |
| MslI | CAYNNNNRTG | 5 cut(s) | 156, 728, 1563, 3012, 3056 |
| Msp20I | TGGCCA | 2 cut(s) | 1607, 3038 |
| MspA1I | CMGCKG | 1 cut(s) | 2686 |
| MspI | CCGG | 5 cut(s) | 228, 245, 277, 1726, 2470 |
| MspR9I | CCNGG | 1 cut(s) | 3040 |
| Mva1269I | GAATGC | 1 cut(s) | 2737 |
| MvaI | CCWGG | 1 cut(s) | 3040 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 167, 197, 206, 282, 794, 1613, 2243 |
| NcoI | CCATGG | 1 cut(s) | 2151 |
| NdeI | CATATG | 3 cut(s) | 885, 964, 1438 |
| NdeII | GATC | 6 cut(s) | 975, 1038, 1571, 1803, 2387, 2818 |
| NlaIV | GGNNCC | 4 cut(s) | 306, 402, 1755, 2363 |
| NmuCI | GTSAC | 5 cut(s) | 698, 934, 1238, 1945, 2416 |
| NsiI | ATGCAT | 1 cut(s) | 2080 |
| NspI | RCATGY | 3 cut(s) | 719, 2046, 2078 |
| PaeI | GCATGC | 2 cut(s) | 719, 2046 |
| PaeR7I | CTCGAG | 1 cut(s) | 1685 |
| PagI | TCATGA | 3 cut(s) | 51, 2332, 2434 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1234 |
| PctI | GAATGC | 1 cut(s) | 2737 |
| PdmI | GAANNNNTTC | 3 cut(s) | 547, 2031, 2454 |
| PfeI | GAWTC | 6 cut(s) | 48, 685, 1023, 1250, 1281, 1369 |
| PflFI | GACNNNGTC | 1 cut(s) | 643 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1721 |
| PkrI | GCNGC | 9 cut(s) | 134, 202, 205, 208, 721, 1236, 1317, 2685, 3025 |
| PleI | GAGTC | 2 cut(s) | 652, 2693 |
| PpsI | GAGTC | 2 cut(s) | 652, 2693 |
| Ppu21I | YACGTR | 1 cut(s) | 740 |
| PshBI | ATTAAT | 1 cut(s) | 462 |
| Psp124BI | GAGCTC | 1 cut(s) | 8 |
| Psp6I | CCWGG | 1 cut(s) | 3038 |
| PspEI | GGTNACC | 1 cut(s) | 1849 |
| PspGI | CCWGG | 1 cut(s) | 3038 |
| PspN4I | GGNNCC | 4 cut(s) | 306, 402, 1755, 2363 |
| PspPI | GGNCC | 3 cut(s) | 198, 912, 2361 |
| PspXI | VCTCGAGB | 1 cut(s) | 1685 |
| PstI | CTGCAG | 1 cut(s) | 1483 |
| PstNI | CAGNNNCTG | 2 cut(s) | 647, 1478 |
| PsuI | RGATCY | 2 cut(s) | 1571, 2387 |
| PsyI | GACNNNGTC | 1 cut(s) | 643 |
| RsaI | GTAC | 8 cut(s) | 14, 527, 742, 1067, 1455, 2149, 2165, 2761 |
| RsaNI | GTAC | 8 cut(s) | 13, 526, 741, 1066, 1454, 2148, 2164, 2760 |
| RseI | CAYNNNNRTG | 5 cut(s) | 156, 728, 1563, 3012, 3056 |
| SacI | GAGCTC | 1 cut(s) | 8 |
| SatI | GCNGC | 9 cut(s) | 133, 201, 204, 207, 720, 1235, 1316, 2684, 3024 |
| Sau3AI | GATC | 6 cut(s) | 975, 1038, 1571, 1803, 2387, 2818 |
| Sau96I | GGNCC | 3 cut(s) | 198, 912, 2361 |
| ScaI | AGTACT | 2 cut(s) | 527, 2165 |
| SchI | GAGTC | 2 cut(s) | 653, 2693 |
| ScrFI | CCNGG | 1 cut(s) | 3040 |
| SduI | GDGCHC | 4 cut(s) | 8, 1873, 2143, 2886 |
| SfaNI | GCATC | 5 cut(s) | 250, 457, 1917, 1982, 2073 |
| SfcI | CTRYAG | 3 cut(s) | 864, 1303, 1479 |
| Sfr274I | CTCGAG | 1 cut(s) | 1685 |
| SlaI | CTCGAG | 1 cut(s) | 1685 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 156, 728, 1563, 3012, 3056 |
| SmlI | CTYRAG | 3 cut(s) | 575, 831, 1685 |
| SmoI | CTYRAG | 3 cut(s) | 575, 831, 1685 |
| SphI | GCATGC | 2 cut(s) | 719, 2046 |
| SspI | AATATT | 3 cut(s) | 619, 904, 1636 |
| SspMI | CTAG | 2 cut(s) | 1185, 1922 |
| SstI | GAGCTC | 1 cut(s) | 8 |
| StyD4I | CCNGG | 1 cut(s) | 3038 |
| StyI | CCWWGG | 3 cut(s) | 2151, 2547, 2928 |
| TaiI | ACGT | 4 cut(s) | 742, 1231, 1962, 2680 |
| TaqI | TCGA | 9 cut(s) | 30, 46, 120, 237, 688, 978, 1041, 1686, 2702 |
| TaqII | GACCGA | 1 cut(s) | 2711 |
| TatI | WGTACW | 4 cut(s) | 12, 525, 2163, 2759 |
| TauI | GCSGC | 3 cut(s) | 203, 206, 209 |
| TfiI | GAWTC | 6 cut(s) | 48, 685, 1023, 1250, 1281, 1369 |
| TscAI | CASTG | 4 cut(s) | 418, 661, 2617, 2828 |
| TseFI | GTSAC | 5 cut(s) | 698, 934, 1238, 1945, 2416 |
| TseI | GCWGC | 6 cut(s) | 132, 719, 1234, 1315, 2683, 3023 |
| Tsp45I | GTSAC | 5 cut(s) | 698, 934, 1238, 1945, 2416 |
| TspGWI | ACGGA | 4 cut(s) | 231, 255, 1778, 2211 |
| TspRI | CASTG | 4 cut(s) | 418, 661, 2617, 2828 |
| Tth111I | GACNNNGTC | 1 cut(s) | 643 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1721 |
| VspI | ATTAAT | 1 cut(s) | 462 |
| XapI | RAATTY | 5 cut(s) | 406, 1830, 2027, 3061, 3068 |
| XceI | RCATGY | 3 cut(s) | 719, 2046, 2078 |
| XhoI | CTCGAG | 1 cut(s) | 1685 |
| XmiI | GTMKAC | 1 cut(s) | 3048 |
| XmnI | GAANNNNTTC | 3 cut(s) | 547, 2031, 2454 |
| XspI | CTAG | 2 cut(s) | 1185, 1922 |
| ZrmI | AGTACT | 2 cut(s) | 527, 2165 |
| Zsp2I | ATGCAT | 1 cut(s) | 2080 |