Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 227 |
| AcoI | YGGCCR | 1 cut(s) | 224 |
| AcsI | RAATTY | 2 cut(s) | 138, 352 |
| AjnI | CCWGG | 1 cut(s) | 399 |
| AluBI | AGCT | 4 cut(s) | 82, 97, 410, 426 |
| AluI | AGCT | 4 cut(s) | 82, 97, 410, 426 |
| Ama87I | CYCGRG | 1 cut(s) | 144 |
| AoxI | GGCC | 1 cut(s) | 224 |
| ApoI | RAATTY | 2 cut(s) | 138, 352 |
| AspS9I | GGNCC | 1 cut(s) | 173 |
| AsuHPI | GGTGA | 1 cut(s) | 65 |
| AvaI | CYCGRG | 1 cut(s) | 144 |
| AvaII | GGWCC | 1 cut(s) | 173 |
| BciT130I | CCWGG | 1 cut(s) | 401 |
| BfaI | CTAG | 1 cut(s) | 213 |
| BfmI | CTRYAG | 2 cut(s) | 61, 177 |
| BisI | GCNGC | 1 cut(s) | 227 |
| BlsI | GCNGC | 1 cut(s) | 228 |
| Bme1390I | CCNGG | 1 cut(s) | 401 |
| Bme18I | GGWCC | 1 cut(s) | 173 |
| BmeT110I | CYCGRG | 1 cut(s) | 144 |
| BmgT120I | GGNCC | 1 cut(s) | 173 |
| BmrFI | CCNGG | 1 cut(s) | 401 |
| BsaJI | CCNNGG | 1 cut(s) | 400 |
| BseBI | CCWGG | 1 cut(s) | 401 |
| BseDI | CCNNGG | 1 cut(s) | 400 |
| BseMII | CTCAG | 1 cut(s) | 276 |
| BshFI | GGCC | 1 cut(s) | 226 |
| BsiHKCI | CYCGRG | 1 cut(s) | 144 |
| BsnI | GGCC | 1 cut(s) | 226 |
| BsoBI | CYCGRG | 1 cut(s) | 144 |
| BspACI | CCGC | 1 cut(s) | 227 |
| BspANI | GGCC | 1 cut(s) | 226 |
| BspCNI | CTCAG | 1 cut(s) | 277 |
| BssECI | CCNNGG | 1 cut(s) | 400 |
| Bst2UI | CCWGG | 1 cut(s) | 401 |
| Bst4CI | ACNGT | 1 cut(s) | 311 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 107 |
| BstC8I | GCNNGC | 2 cut(s) | 99, 438 |
| BstDEI | CTNAG | 1 cut(s) | 285 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 107, 437 |
| BstNI | CCWGG | 1 cut(s) | 401 |
| BstNSI | RCATGY | 1 cut(s) | 440 |
| BstSCI | CCNGG | 1 cut(s) | 399 |
| BstSFI | CTRYAG | 2 cut(s) | 61, 177 |
| BsuRI | GGCC | 1 cut(s) | 226 |
| BtsIMutI | CAGTG | 1 cut(s) | 282 |
| Cac8I | GCNNGC | 2 cut(s) | 99, 438 |
| Cfr13I | GGNCC | 1 cut(s) | 173 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 66, 101 |
| CviAII | CATG | 1 cut(s) | 437 |
| CviJI | RGCY | 6 cut(s) | 70, 82, 97, 226, 410, 426 |
| CviKI_1 | RGCY | 6 cut(s) | 70, 82, 97, 226, 410, 426 |
| DdeI | CTNAG | 1 cut(s) | 285 |
| DraI | TTTAAA | 1 cut(s) | 358 |
| EaeI | YGGCCR | 1 cut(s) | 224 |
| Eco32I | GATATC | 1 cut(s) | 291 |
| Eco47I | GGWCC | 1 cut(s) | 173 |
| Eco88I | CYCGRG | 1 cut(s) | 144 |
| EcoRII | CCWGG | 1 cut(s) | 399 |
| EcoRV | GATATC | 1 cut(s) | 291 |
| EcoT22I | ATGCAT | 1 cut(s) | 438 |
| FaeI | CATG | 1 cut(s) | 440 |
| FaiI | YATR | 9 cut(s) | 38, 63, 135, 208, 279, 307, 366, 434, 438 |
| FatI | CATG | 1 cut(s) | 436 |
| Fnu4HI | GCNGC | 1 cut(s) | 227 |
| Fsp4HI | GCNGC | 1 cut(s) | 227 |
| FspBI | CTAG | 1 cut(s) | 213 |
| GluI | GCNGC | 1 cut(s) | 227 |
| HaeIII | GGCC | 1 cut(s) | 226 |
| Hin1II | CATG | 1 cut(s) | 440 |
| HinfI | GANTC | 1 cut(s) | 216 |
| HphI | GGTGA | 1 cut(s) | 65 |
| Hpy188I | TCNGA | 3 cut(s) | 116, 351, 416 |
| HpyAV | CCTTC | 2 cut(s) | 30, 85 |
| HpyCH4III | ACNGT | 1 cut(s) | 311 |
| HpyCH4V | TGCA | 3 cut(s) | 110, 206, 436 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 107, 437 |
| HpyF3I | CTNAG | 1 cut(s) | 285 |
| Hsp92II | CATG | 1 cut(s) | 440 |
| LmnI | GCTCC | 1 cut(s) | 94 |
| LpnPI | CCDG | 7 cut(s) | 83, 156, 189, 311, 386, 404, 413 |
| MaeI | CTAG | 1 cut(s) | 213 |
| MaeIII | GTNAC | 2 cut(s) | 247, 280 |
| MboII | GAAGA | 1 cut(s) | 49 |
| MluCI | AATT | 6 cut(s) | 102, 138, 184, 231, 352, 370 |
| MnlI | CCTC | 1 cut(s) | 81 |
| Mph1103I | ATGCAT | 1 cut(s) | 438 |
| MseI | TTAA | 4 cut(s) | 270, 357, 387, 442 |
| MslI | CAYNNNNRTG | 1 cut(s) | 388 |
| MspR9I | CCNGG | 1 cut(s) | 401 |
| MvaI | CCWGG | 1 cut(s) | 401 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 107, 437 |
| NlaIII | CATG | 1 cut(s) | 440 |
| NmuCI | GTSAC | 1 cut(s) | 280 |
| NsiI | ATGCAT | 1 cut(s) | 438 |
| NspI | RCATGY | 1 cut(s) | 440 |
| PaeI | GCATGC | 1 cut(s) | 440 |
| PfeI | GAWTC | 1 cut(s) | 216 |
| PkrI | GCNGC | 1 cut(s) | 228 |
| Psp6I | CCWGG | 1 cut(s) | 399 |
| PspGI | CCWGG | 1 cut(s) | 399 |
| PspPI | GGNCC | 1 cut(s) | 173 |
| RseI | CAYNNNNRTG | 1 cut(s) | 388 |
| SaqAI | TTAA | 4 cut(s) | 270, 357, 387, 442 |
| SatI | GCNGC | 1 cut(s) | 227 |
| Sau96I | GGNCC | 1 cut(s) | 173 |
| ScrFI | CCNGG | 1 cut(s) | 401 |
| SetI | ASST | 5 cut(s) | 22, 84, 99, 412, 428 |
| SfcI | CTRYAG | 2 cut(s) | 61, 177 |
| SinI | GGWCC | 1 cut(s) | 173 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 388 |
| SphI | GCATGC | 1 cut(s) | 440 |
| Sse9I | AATT | 6 cut(s) | 102, 138, 184, 231, 352, 370 |
| SsiI | CCGC | 1 cut(s) | 227 |
| SspMI | CTAG | 1 cut(s) | 213 |
| StyD4I | CCNGG | 1 cut(s) | 399 |
| TaaI | ACNGT | 1 cut(s) | 311 |
| TasI | AATT | 6 cut(s) | 102, 138, 184, 231, 352, 370 |
| TauI | GCSGC | 1 cut(s) | 229 |
| TfiI | GAWTC | 1 cut(s) | 216 |
| Tru1I | TTAA | 4 cut(s) | 270, 357, 387, 442 |
| Tru9I | TTAA | 4 cut(s) | 270, 357, 387, 442 |
| TscAI | CASTG | 1 cut(s) | 289 |
| TseFI | GTSAC | 1 cut(s) | 280 |
| Tsp45I | GTSAC | 1 cut(s) | 280 |
| TspRI | CASTG | 1 cut(s) | 289 |
| VpaK11BI | GGWCC | 1 cut(s) | 173 |
| XapI | RAATTY | 2 cut(s) | 138, 352 |
| XceI | RCATGY | 1 cut(s) | 440 |
| XspI | CTAG | 1 cut(s) | 213 |
| Zsp2I | ATGCAT | 1 cut(s) | 438 |