MD04G1043400.v1.1

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000912
Physical Location & Seq
Reverse (-)
5111792 .. 5113197
1406 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1043400.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGACCTTCCGATTCATCGACGTCGGAGCCTCTTCTCCTTCCTCTTCTTTTTCTTCTAGGGCTCCGAACTGCAACCAACTCTCCTGCTTCTTCAGTTCTGTGCCATCCAAAGCCCCATACCTTCATCGTCCTTCCAACACTTCTTCTTTGTCACTCTCTCGGAGCCGCATGGACTGCTCTCTAAGAGCCGCCATCGAAGATACCAATGGTACATGCCACGGCAAAGTCGAAATCGGGCATTCGATTTTGACGGCTTTGTCGCCGTTGGACGGCCGTTATTGGGGTAAAGTCAAGGAATTGGCTCCTTTTATGAGCGAATATGCCCTAATCTACTACAGAGTTTTCGTTGAGATAAAATGGCTGTTGAAACTTTCACAAATCCCTGAGGTCACAGAGGTGCCCAGTTTCAGTGGAGATGCTCAATCTTACTTGCAAGGAATCATCGATGGATTTAGCGTCGATGATGCAGCGGAAATAAAGAAGATTGAGAAAATTACTAATCATGATGTGAAAGCAGTGGAGTATTTTTTGAAACAGAAATGCAGTTCGCATCCAGAGATAGCTAAGGTGCTTGAGTTTTTCCATTTTGCTTGCACATCTGAGGACATCAACAATCTTGCTCATGCGTTGATGTTGAAAGATGCTATCAACAATGTTATGTTTCCCGTCATGGATGAGTTGGTTGAGGCAATTTGTGACATGTCAAAGGATAACGCGTCCGTTCCCATGCTTTCTCGCACGCACGGTCAGACTGCCTCACCCACCACTTTGGGGAAGGAAATGGCAATTTTCGCTGTCAGGTTAAGCATACAAAGGCGCAGAATTTCTCAGGTTGAAATCATGGGGAAGTTTGCTGGTGCAGTAGGAAATTACAATGCTCATCTTGTTGCATATCCTGATGTTGATTGGCCTCAGATTGCTGAAGAGTTTGTAACATCTCTTGGAGTGAGTTTTAATCCTTACGTTACTCAGATTGAGACTCATGACGAGGAGGAGGCGAAGACGCTTGAGAGCTCTCCAATTCCATGTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

345

Amino Acids

38.17

Weight (kDa)

5.5

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_1 PF00206 123 - 330 2e-33 Lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 256
AatII GACGTC 1 cut(s) 24
AccB1I GGYRCC 1 cut(s) 397
AccII CGCG 1 cut(s) 716
AciI CCGC 3 cut(s) 166, 189, 470
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 1 cut(s) 822
AcuI CTGAAG 2 cut(s) 76, 942
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 1 cut(s) 211
AfiI CCNNNNNNNGG 3 cut(s) 269, 280, 771
AflIII ACRYGT 2 cut(s) 699, 714
AgsI TTSAA 4 cut(s) 367, 532, 637, 836
AleI CACNNNNGTG 1 cut(s) 395
AluBI AGCT 2 cut(s) 563, 1014
AluI AGCT 2 cut(s) 563, 1014
Alw21I GWGCWC 1 cut(s) 1016
Alw26I GTCTC 1 cut(s) 971
AoxI GGCC 2 cut(s) 271, 908
ApeKI GCWGC 1 cut(s) 467
ApoI RAATTY 1 cut(s) 822
AspLEI GCGC 1 cut(s) 819
AsuHPI GGTGA 1 cut(s) 750
AxyI CCTNAGG 1 cut(s) 384
BaeGI GKGCMC 1 cut(s) 402
BanI GGYRCC 1 cut(s) 397
BanII GRGCYC 2 cut(s) 64, 1016
BbsI GAAGAC 1 cut(s) 1007
Bbv12I GWGCWC 1 cut(s) 1016
BbvI GCAGC 1 cut(s) 479
BccI CCATC 3 cut(s) 112, 200, 440
BceAI ACGGC 5 cut(s) 235, 247, 258, 267, 286
BcoDI GTCTC 1 cut(s) 971
BfaI CTAG 1 cut(s) 57
BfmI CTRYAG 1 cut(s) 334
BisI GCNGC 3 cut(s) 166, 189, 468
BlsI GCNGC 3 cut(s) 167, 190, 469
BmiI GGNNCC 5 cut(s) 28, 63, 164, 303, 399
BmrI ACTGGG 1 cut(s) 396
BmsI GCATC 4 cut(s) 406, 454, 559, 631
BmuI ACTGGG 1 cut(s) 396
BpiI GAAGAC 1 cut(s) 1007
Bpu10I CCTNAGC 1 cut(s) 564
BpuEI CTTGAG 2 cut(s) 593, 1028
Bsa29I ATCGAT 1 cut(s) 444
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 1 cut(s) 217
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bsc4I CCNNNNNNNGG 3 cut(s) 269, 280, 771
Bse1I ACTGG 1 cut(s) 402
Bse21I CCTNAGG 1 cut(s) 384
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 3 cut(s) 104, 550, 679
BseLI CCNNNNNNNGG 3 cut(s) 269, 280, 771
BseMII CTCAG 5 cut(s) 375, 591, 842, 926, 983
BseNI ACTGG 1 cut(s) 402
BseRI GAGGAG 2 cut(s) 1004, 1007
BseSI GKGCMC 1 cut(s) 402
BseX3I CGGCCG 1 cut(s) 271
BseXI GCAGC 1 cut(s) 479
BsgI GTGCAG 1 cut(s) 879
Bsh1236I CGCG 1 cut(s) 716
Bsh1285I CGRYCG 1 cut(s) 274
BshFI GGCC 2 cut(s) 273, 910
BshNI GGYRCC 1 cut(s) 397
BshVI ATCGAT 1 cut(s) 444
BsiEI CGRYCG 1 cut(s) 274
BsiHKAI GWGCWC 1 cut(s) 1016
BslI CCNNNNNNNGG 3 cut(s) 269, 280, 771
BsmAI GTCTC 1 cut(s) 971
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 2 cut(s) 273, 910
Bsp1286I GDGCHC 3 cut(s) 64, 402, 1016
BspACI CCGC 3 cut(s) 166, 189, 470
BspANI GGCC 2 cut(s) 273, 910
BspCNI CTCAG 5 cut(s) 376, 592, 841, 925, 982
BspDI ATCGAT 1 cut(s) 444
BspFNI CGCG 1 cut(s) 716
BspHI TCATGA 2 cut(s) 502, 982
BspLI GGNNCC 5 cut(s) 28, 63, 164, 303, 399
BspT107I GGYRCC 1 cut(s) 397
BsrI ACTGG 1 cut(s) 402
BssECI CCNNGG 1 cut(s) 217
BssNI GRCGYC 1 cut(s) 21
Bst4CI ACNGT 1 cut(s) 746
Bst6I CTCTTC 3 cut(s) 37, 49, 918
BstACI GRCGYC 1 cut(s) 21
BstAPI GCANNNNNTGC 1 cut(s) 174
BstC8I GCNNGC 2 cut(s) 592, 740
BstDEI CTNAG 7 cut(s) 182, 384, 564, 600, 828, 912, 969
BstDSI CCRYGG 1 cut(s) 217
BstF5I GGATG 3 cut(s) 104, 550, 679
BstFNI CGCG 1 cut(s) 716
BstHHI GCGC 1 cut(s) 819
BstMAI GTCTC 1 cut(s) 971
BstMCI CGRYCG 1 cut(s) 274
BstMWI GCNNNNNNNGC 2 cut(s) 174, 791
BstNSI RCATGY 2 cut(s) 216, 703
BstSFI CTRYAG 1 cut(s) 334
BstSLI GKGCMC 1 cut(s) 402
BstUI CGCG 1 cut(s) 716
BstV1I GCAGC 1 cut(s) 479
BstV2I GAAGAC 1 cut(s) 1007
BstXI CCANNNNNNTGG 1 cut(s) 769
BstZI CGGCCG 1 cut(s) 271
Bsu15I ATCGAT 1 cut(s) 444
Bsu36I CCTNAGG 1 cut(s) 384
BsuRI GGCC 2 cut(s) 273, 910
BsuTUI ATCGAT 1 cut(s) 444
BtgI CCRYGG 1 cut(s) 217
BtsCI GGATG 3 cut(s) 104, 550, 679
BtsI GCAGTG 1 cut(s) 522
BtsIMutI CAGTG 2 cut(s) 415, 522
Cac8I GCNNGC 2 cut(s) 592, 740
CciI TCATGA 2 cut(s) 502, 982
CfoI GCGC 1 cut(s) 819
ClaI ATCGAT 1 cut(s) 444
CseI GACGC 3 cut(s) 445, 705, 1012
Csp6I GTAC 1 cut(s) 210
CviQI GTAC 1 cut(s) 210
DdeI CTNAG 7 cut(s) 182, 384, 564, 600, 828, 912, 969
DrdI GACNNNNNNGTC 1 cut(s) 256
DseDI GACNNNNNNGTC 1 cut(s) 256
EaeI YGGCCR 1 cut(s) 271
EagI CGGCCG 1 cut(s) 271
Eam1104I CTCTTC 3 cut(s) 37, 49, 918
EarI CTCTTC 3 cut(s) 37, 49, 918
Ecl136II GAGCTC 1 cut(s) 1014
EclXI CGGCCG 1 cut(s) 271
Eco24I GRGCYC 2 cut(s) 64, 1016
Eco52I CGGCCG 1 cut(s) 271
Eco53kI GAGCTC 1 cut(s) 1014
Eco57I CTGAAG 2 cut(s) 76, 942
Eco81I CCTNAGG 1 cut(s) 384
EcoICRI GAGCTC 1 cut(s) 1014
EcoT38I GRGCYC 2 cut(s) 64, 1016
Fnu4HI GCNGC 3 cut(s) 166, 189, 468
FokI GGATG 3 cut(s) 91, 537, 686
FriOI GRGCYC 2 cut(s) 64, 1016
Fsp4HI GCNGC 3 cut(s) 166, 189, 468
FspBI CTAG 1 cut(s) 57
GlaI GCGC 1 cut(s) 818
GluI GCNGC 3 cut(s) 166, 189, 468
HaeIII GGCC 2 cut(s) 273, 910
HgaI GACGC 3 cut(s) 445, 705, 1012
HhaI GCGC 1 cut(s) 819
Hin1I GRCGYC 1 cut(s) 21
Hin6I GCGC 1 cut(s) 817
HinP1I GCGC 1 cut(s) 817
HinfI GANTC 3 cut(s) 12, 438, 979
HphI GGTGA 1 cut(s) 750
Hpy188I TCNGA 9 cut(s) 11, 26, 66, 162, 601, 750, 915, 972, 1034
Hpy188III TCNNGA 4 cut(s) 503, 554, 896, 983
Hpy99I CGWCG 3 cut(s) 23, 26, 461
HpyAV CCTTC 5 cut(s) 16, 48, 131, 141, 769
HpyCH4III ACNGT 1 cut(s) 746
HpyCH4IV ACGT 2 cut(s) 21, 963
HpyCH4V TGCA 7 cut(s) 72, 433, 467, 543, 594, 860, 890
HpyF10VI GCNNNNNNNGC 2 cut(s) 174, 791
HpyF3I CTNAG 7 cut(s) 182, 384, 564, 600, 828, 912, 969
HpySE526I ACGT 2 cut(s) 21, 963
Hsp92I GRCGYC 1 cut(s) 21
HspAI GCGC 1 cut(s) 817
LmnI GCTCC 4 cut(s) 26, 67, 162, 307
LpnPI CCDG 8 cut(s) 97, 396, 415, 567, 784, 815, 840, 909
Lsp1109I GCAGC 1 cut(s) 479
LweI GCATC 4 cut(s) 406, 454, 559, 631
MaeI CTAG 1 cut(s) 57
MaeII ACGT 2 cut(s) 21, 963
MaeIII GTNAC 5 cut(s) 150, 388, 695, 931, 964
MboII GAAGA 9 cut(s) 24, 36, 45, 82, 135, 209, 493, 935, 1012
MhlI GDGCHC 3 cut(s) 64, 402, 1016
MluCI AATT 7 cut(s) 296, 492, 690, 786, 822, 868, 1020
MluI ACGCGT 1 cut(s) 714
MlyI GAGTC 1 cut(s) 973
MmeI TCCRAC 3 cut(s) 4, 159, 246
MseI TTAA 2 cut(s) 803, 954
MslI CAYNNNNRTG 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 470
Mva1269I GAATGC 1 cut(s) 238
MvnI CGCG 1 cut(s) 716
MwoI GCNNNNNNNGC 2 cut(s) 174, 791
NlaIV GGNNCC 5 cut(s) 28, 63, 164, 303, 399
NmuCI GTSAC 3 cut(s) 150, 388, 695
NspI RCATGY 2 cut(s) 216, 703
OliI CACNNNNGTG 1 cut(s) 395
PagI TCATGA 2 cut(s) 502, 982
PciI ACATGT 1 cut(s) 699
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 1 cut(s) 238
PfeI GAWTC 2 cut(s) 12, 438
PkrI GCNGC 3 cut(s) 167, 190, 469
PleI GAGTC 1 cut(s) 973
PpsI GAGTC 1 cut(s) 973
PscI ACATGT 1 cut(s) 699
Psp124BI GAGCTC 1 cut(s) 1016
PspN4I GGNNCC 5 cut(s) 28, 63, 164, 303, 399
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
RseI CAYNNNNRTG 1 cut(s) 395
SacI GAGCTC 1 cut(s) 1016
SaqAI TTAA 2 cut(s) 803, 954
SatI GCNGC 3 cut(s) 166, 189, 468
SchI GAGTC 1 cut(s) 973
SduI GDGCHC 3 cut(s) 64, 402, 1016
SfaNI GCATC 4 cut(s) 406, 454, 559, 631
SfcI CTRYAG 1 cut(s) 334
SmiMI CAYNNNNRTG 1 cut(s) 395
SmlI CTYRAG 2 cut(s) 572, 1007
SmoI CTYRAG 2 cut(s) 572, 1007
Sse9I AATT 7 cut(s) 296, 492, 690, 786, 822, 868, 1020
SsiI CCGC 3 cut(s) 166, 189, 470
SspMI CTAG 1 cut(s) 57
SstI GAGCTC 1 cut(s) 1016
TaaI ACNGT 1 cut(s) 746
TaiI ACGT 2 cut(s) 24, 966
TaqI TCGA 6 cut(s) 18, 195, 228, 242, 444, 459
TasI AATT 7 cut(s) 296, 492, 690, 786, 822, 868, 1020
TauI GCSGC 2 cut(s) 168, 191
TfiI GAWTC 2 cut(s) 12, 438
Tru1I TTAA 2 cut(s) 803, 954
Tru9I TTAA 2 cut(s) 803, 954
TscAI CASTG 2 cut(s) 415, 522
TseFI GTSAC 3 cut(s) 150, 388, 695
TseI GCWGC 1 cut(s) 467
Tsp45I GTSAC 3 cut(s) 150, 388, 695
TspDTI ATGAA 2 cut(s) 4, 113
TspGWI ACGGA 1 cut(s) 709
TspRI CASTG 2 cut(s) 415, 522
XapI RAATTY 1 cut(s) 822
XceI RCATGY 2 cut(s) 216, 703
XspI CTAG 1 cut(s) 57
ZraI GACGTC 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.