Rw7G006850

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000912
Physical Location & Seq
Forward (+)
5869404 .. 5871274
1871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G006850.1

Sequence Viewer

Length: 1194 bp
ATGGGATCATCTTCTAGTGTTATGCCACCCAAATATCAAAGACCAACCTCAAGCTTGTATCTTCATTCACGTGATGCTTTGTCTTCCTTTGGCAGAGGAATTTGTGCCCTAAGGGACACTGATGCTGAAGATTTCAACTTTTCATGGGCGATCGGGAATAGTATGCATCTGCGGACAGACGCAAGTTATGCCAAGGTCATGAAATTCGGTGAACTATCTTCTTCAACGGGTGAATATGCGATGATCTACTACAGAATTCTAGTTCAGGTCAAATGGTTGTTGAAACTTTCAGAGATCCCAGAATTCAGAGACGTACCTAGTTTCAGTGAGCATGCTCAGTGTTACTTACAAGGAATTATTGATCGATTTTGCATGGTGGAAGCCATAGAAATGACAGAGAGGTTGGAGAAAAAAACAAACCATGATCATGTTAAAGCAGTGGAATACTTCTTGAAGCTGAGATGCAGATCAAGACCAGAGATAGCTAAGGTGCTCGATACTTTTCATTTTGCTTGCACATCAGAGGACATCAACAGTCTTGCGCATACGTTAATGTTAAAAGATGCTATGAACAATGTTATATTTCCTGTCATGGACGATCTGATCAAGGCAATGTCTACCATGTCAAGGGACAATGCTTCCATTCCAATTCTTTCTCGCAGGGACGGAAACACGGTCTCACCCACAACTTTGGGGAAGGAAATGGCCAATTTTGCTGTCAGGCTAGGCATACAGAAGCACAAACTTTCTCAAGTGAAGATAATGGGGAAGTTTCCTGGTGCTGTAGGAAGTTCCAATGCTCATTATGTTACATATCCTCATATTAATTGGCCGCAAATTGCTGAGGAATTCGTGACTTCTCTTGGAGTGAGTTTCAATCCTTATGTTACTGAGGCCGAGACTTACGACTACATGCCAAAACTTTTCAATGCTATTAACCGATTCAATAGTATATTGATCGACTTTGATAAAGATATGTGGGACTACTTACATTTTGGTTACTTCAAGCAAAGATTAGTATGTTCTGCTCTTGCCTACAAGAGTACACTTAAGAATATAGCAAAGATTCAGATTGATGAAGCTCTCGTGAGTGAAGAGTTGAATCACTCTTGGGAGGTACTTGCTGAAGCAGTACAAGCTGTAATTCGAAGATATGGTGTTCCTGCGAGACCTATGAGAAGCTTAAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

397

Amino Acids

45.26

Weight (kDa)

8.1

Isoelectric Point (pI)

44.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_1 PF00206 139 - 338 2.4e-21 Lyase
ASL_C PF08328 340 - 388 9.9e-12 Adenylosuccinate lyase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 543
AccI GTMKAC 1 cut(s) 617
AciI CCGC 2 cut(s) 172, 833
AclWI GGATC 2 cut(s) 13, 289
AcoI YGGCCR 2 cut(s) 705, 830
AcsI RAATTY 5 cut(s) 99, 203, 255, 302, 848
AcuI CTGAAG 2 cut(s) 147, 1146
AcvI CACGTG 1 cut(s) 71
AfaI GTAC 4 cut(s) 315, 1045, 1119, 1134
AfiI CCNNNNNNNGG 1 cut(s) 627
AflII CTTAAG 1 cut(s) 1049
AgsI TTSAA 9 cut(s) 136, 225, 283, 454, 877, 928, 946, 1006, 1102
AjnI CCWGG 1 cut(s) 775
AluBI AGCT 6 cut(s) 54, 457, 485, 1082, 1139, 1182
AluI AGCT 6 cut(s) 54, 457, 485, 1082, 1139, 1182
Alw21I GWGCWC 1 cut(s) 495
Alw26I GTCTC 4 cut(s) 303, 682, 893, 1162
AlwI GGATC 2 cut(s) 13, 289
AoxI GGCC 3 cut(s) 705, 830, 894
ApoI RAATTY 5 cut(s) 99, 203, 255, 302, 848
AseI ATTAAT 1 cut(s) 825
AspLEI GCGC 1 cut(s) 544
AsuHPI GGTGA 3 cut(s) 221, 242, 672
AsuII TTCGAA 1 cut(s) 1147
AxyI CCTNAGG 1 cut(s) 110
BaeGI GKGCMC 1 cut(s) 109
BalI TGGCCA 1 cut(s) 707
BauI CACGAG 1 cut(s) 1085
BbrPI CACGTG 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 495
BbvCI CCTCAGC 1 cut(s) 843
BciT130I CCWGG 1 cut(s) 777
BclI TGATCA 2 cut(s) 424, 603
BcoDI GTCTC 4 cut(s) 303, 682, 893, 1162
BfaI CTAG 4 cut(s) 15, 260, 318, 725
BfmI CTRYAG 2 cut(s) 250, 783
BfrI CTTAAG 1 cut(s) 1049
BisI GCNGC 1 cut(s) 833
BlsI GCNGC 1 cut(s) 834
Bme1390I CCNGG 1 cut(s) 777
BmrFI CCNGG 1 cut(s) 777
BmsI GCATC 5 cut(s) 64, 112, 175, 452, 553
BpiI GAAGAC 1 cut(s) 75
Bpu10I CCTNAGC 2 cut(s) 486, 843
Bpu14I TTCGAA 1 cut(s) 1147
BpuEI CTTGAG 2 cut(s) 34, 735
Bsa29I ATCGAT 1 cut(s) 364
BsaAI YACGTR 1 cut(s) 71
BsaBI GATNNNNATC 1 cut(s) 466
BsaI GGTCTC 2 cut(s) 682, 1162
BsaJI CCNNGG 1 cut(s) 192
Bsc4I CCNNNNNNNGG 1 cut(s) 627
Bse21I CCTNAGG 1 cut(s) 110
Bse3DI GCAATG 1 cut(s) 618
Bse8I GATNNNNATC 1 cut(s) 466
BseBI CCWGG 1 cut(s) 777
BseCI ATCGAT 1 cut(s) 364
BseDI CCNNGG 1 cut(s) 192
BseJI GATNNNNATC 1 cut(s) 466
BseLI CCNNNNNNNGG 1 cut(s) 627
BseMI GCAATG 1 cut(s) 618
BseMII CTCAG 4 cut(s) 350, 449, 834, 882
BseSI GKGCMC 1 cut(s) 109
Bsh1285I CGRYCG 1 cut(s) 153
BshFI GGCC 3 cut(s) 707, 832, 896
BshVI ATCGAT 1 cut(s) 364
BsiEI CGRYCG 1 cut(s) 153
BsiHKAI GWGCWC 1 cut(s) 495
BslFI GGGAC 4 cut(s) 128, 644, 677, 995
BslI CCNNNNNNNGG 1 cut(s) 627
BsmAI GTCTC 4 cut(s) 303, 682, 893, 1162
BsmBI CGTCTC 1 cut(s) 303
BsmFI GGGAC 4 cut(s) 128, 644, 677, 995
BsnI GGCC 3 cut(s) 707, 832, 896
Bso31I GGTCTC 2 cut(s) 682, 1162
Bsp119I TTCGAA 1 cut(s) 1147
Bsp1286I GDGCHC 2 cut(s) 109, 495
BspACI CCGC 2 cut(s) 172, 833
BspANI GGCC 3 cut(s) 707, 832, 896
BspCNI CTCAG 4 cut(s) 349, 450, 835, 883
BspDI ATCGAT 1 cut(s) 364
BspHI TCATGA 1 cut(s) 198
BspPI GGATC 2 cut(s) 13, 289
BspT104I TTCGAA 1 cut(s) 1147
BspTI CTTAAG 1 cut(s) 1049
BspTNI GGTCTC 2 cut(s) 682, 1162
BsrDI GCAATG 1 cut(s) 618
BssECI CCNNGG 1 cut(s) 192
BssSI CACGAG 1 cut(s) 1085
BssT1I CCWWGG 1 cut(s) 192
Bst2BI CACGAG 1 cut(s) 1085
Bst2UI CCWGG 1 cut(s) 777
Bst4CI ACNGT 2 cut(s) 536, 676
Bst6I CTCTTC 1 cut(s) 1089
BstAFI CTTAAG 1 cut(s) 1049
BstAPI GCANNNNNTGC 1 cut(s) 188
BstBAI YACGTR 1 cut(s) 71
BstBI TTCGAA 1 cut(s) 1147
BstC8I GCNNGC 2 cut(s) 333, 514
BstDEI CTNAG 6 cut(s) 110, 336, 458, 486, 843, 891
BstHHI GCGC 1 cut(s) 544
BstMAI GTCTC 4 cut(s) 303, 682, 893, 1162
BstMCI CGRYCG 1 cut(s) 153
BstMWI GCNNNNNNNGC 3 cut(s) 188, 713, 1136
BstNI CCWGG 1 cut(s) 777
BstNSI RCATGY 2 cut(s) 335, 916
BstSCI CCNGG 1 cut(s) 775
BstSFI CTRYAG 2 cut(s) 250, 783
BstSLI GKGCMC 1 cut(s) 109
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 294
BstXI CCANNNNNNTGG 1 cut(s) 691
BstYI RGATCY 1 cut(s) 294
Bsu15I ATCGAT 1 cut(s) 364
Bsu36I CCTNAGG 1 cut(s) 110
BsuRI GGCC 3 cut(s) 707, 832, 896
BsuTUI ATCGAT 1 cut(s) 364
BtgZI GCGATG 1 cut(s) 254
BtsI GCAGTG 1 cut(s) 444
BtsIMutI CAGTG 4 cut(s) 117, 331, 344, 444
Cac8I GCNNGC 2 cut(s) 333, 514
CciI TCATGA 1 cut(s) 198
CfoI GCGC 1 cut(s) 544
ClaI ATCGAT 1 cut(s) 364
CseI GACGC 1 cut(s) 188
Csp6I GTAC 4 cut(s) 314, 1044, 1118, 1133
CviAII CATG 9 cut(s) 144, 199, 332, 373, 422, 428, 592, 622, 913
CviQI GTAC 4 cut(s) 314, 1044, 1118, 1133
DdeI CTNAG 6 cut(s) 110, 336, 458, 486, 843, 891
EaeI YGGCCR 2 cut(s) 705, 830
Eam1104I CTCTTC 1 cut(s) 1089
EarI CTCTTC 1 cut(s) 1089
Eco130I CCWWGG 1 cut(s) 192
Eco31I GGTCTC 2 cut(s) 682, 1162
Eco57I CTGAAG 2 cut(s) 147, 1146
Eco72I CACGTG 1 cut(s) 71
Eco81I CCTNAGG 1 cut(s) 110
EcoRI GAATTC 3 cut(s) 255, 302, 848
EcoRII CCWGG 1 cut(s) 775
EcoT14I CCWWGG 1 cut(s) 192
EcoT22I ATGCAT 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 192
Esp3I CGTCTC 1 cut(s) 303
FaeI CATG 9 cut(s) 147, 202, 335, 376, 425, 431, 595, 625, 916
FaqI GGGAC 4 cut(s) 128, 644, 677, 995
FatI CATG 9 cut(s) 143, 198, 331, 372, 421, 427, 591, 621, 912
FbaI TGATCA 2 cut(s) 424, 603
FblI GTMKAC 1 cut(s) 617
Fnu4HI GCNGC 1 cut(s) 833
Fsp4HI GCNGC 1 cut(s) 833
FspBI CTAG 4 cut(s) 15, 260, 318, 725
FspI TGCGCA 1 cut(s) 543
GlaI GCGC 1 cut(s) 543
GluI GCNGC 1 cut(s) 833
HaeIII GGCC 3 cut(s) 707, 832, 896
HgaI GACGC 1 cut(s) 188
HhaI GCGC 1 cut(s) 544
Hin1II CATG 9 cut(s) 147, 202, 335, 376, 425, 431, 595, 625, 916
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HindIII AAGCTT 2 cut(s) 52, 1180
HinfI GANTC 3 cut(s) 942, 1066, 1102
HphI GGTGA 3 cut(s) 221, 242, 672
Hpy166II GTNNAC 3 cut(s) 212, 618, 1046
Hpy188I TCNGA 5 cut(s) 292, 308, 523, 603, 1071
Hpy188III TCNNGA 6 cut(s) 154, 199, 451, 471, 853, 1087
Hpy8I GTNNAC 3 cut(s) 212, 618, 1046
HpyAV CCTTC 1 cut(s) 691
HpyCH4III ACNGT 2 cut(s) 536, 676
HpyCH4IV ACGT 3 cut(s) 70, 312, 548
HpyCH4V TGCA 4 cut(s) 166, 372, 465, 516
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 713, 1136
HpyF3I CTNAG 6 cut(s) 110, 336, 458, 486, 843, 891
HpySE526I ACGT 3 cut(s) 70, 312, 548
Hsp92II CATG 9 cut(s) 147, 202, 335, 376, 425, 431, 595, 625, 916
HspAI GCGC 1 cut(s) 542
Ksp22I TGATCA 2 cut(s) 424, 603
LpnPI CCDG 9 cut(s) 251, 312, 489, 600, 646, 706, 762, 789, 1176
LweI GCATC 5 cut(s) 64, 112, 175, 452, 553
MaeI CTAG 4 cut(s) 15, 260, 318, 725
MaeII ACGT 3 cut(s) 70, 312, 548
MaeIII GTNAC 5 cut(s) 341, 808, 853, 886, 998
MboII GAAGA 9 cut(s) 3, 53, 75, 140, 210, 213, 769, 1106, 1161
MflI RGATCY 1 cut(s) 294
MhlI GDGCHC 2 cut(s) 109, 495
MlsI TGGCCA 1 cut(s) 707
MluNI TGGCCA 1 cut(s) 707
MmeI TCCRAC 1 cut(s) 384
MnlI CCTC 8 cut(s) 58, 89, 393, 517, 828, 838, 886, 1108
Mox20I TGGCCA 1 cut(s) 707
Mph1103I ATGCAT 1 cut(s) 168
MscI TGGCCA 1 cut(s) 707
MseI TTAA 7 cut(s) 432, 551, 557, 825, 936, 1050, 1184
MslI CAYNNNNRTG 3 cut(s) 69, 389, 426
Msp20I TGGCCA 1 cut(s) 707
MspCI CTTAAG 1 cut(s) 1049
MspR9I CCNGG 1 cut(s) 777
MvaI CCWGG 1 cut(s) 777
MwoI GCNNNNNNNGC 3 cut(s) 188, 713, 1136
NlaIII CATG 9 cut(s) 147, 202, 335, 376, 425, 431, 595, 625, 916
NmeAIII GCCGAG 1 cut(s) 922
NmuCI GTSAC 1 cut(s) 853
NsbI TGCGCA 1 cut(s) 543
NsiI ATGCAT 1 cut(s) 168
NspI RCATGY 2 cut(s) 335, 916
NspV TTCGAA 1 cut(s) 1147
PaeI GCATGC 1 cut(s) 335
PagI TCATGA 1 cut(s) 198
PfeI GAWTC 3 cut(s) 942, 1066, 1102
PkrI GCNGC 1 cut(s) 834
Ple19I CGATCG 1 cut(s) 153
PmaCI CACGTG 1 cut(s) 71
PmlI CACGTG 1 cut(s) 71
Ppu21I YACGTR 1 cut(s) 71
PshBI ATTAAT 1 cut(s) 825
Psp6I CCWGG 1 cut(s) 775
PspCI CACGTG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 775
PsuI RGATCY 1 cut(s) 294
PvuI CGATCG 1 cut(s) 153
RsaI GTAC 4 cut(s) 315, 1045, 1119, 1134
RsaNI GTAC 4 cut(s) 314, 1044, 1118, 1133
RseI CAYNNNNRTG 3 cut(s) 69, 389, 426
SaqAI TTAA 7 cut(s) 432, 551, 557, 825, 936, 1050, 1184
SatI GCNGC 1 cut(s) 833
ScrFI CCNGG 1 cut(s) 777
SduI GDGCHC 2 cut(s) 109, 495
SfaNI GCATC 5 cut(s) 64, 112, 175, 452, 553
SfcI CTRYAG 2 cut(s) 250, 783
SfuI TTCGAA 1 cut(s) 1147
SmiMI CAYNNNNRTG 3 cut(s) 69, 389, 426
SmlI CTYRAG 3 cut(s) 49, 750, 1049
SmoI CTYRAG 3 cut(s) 49, 750, 1049
SphI GCATGC 1 cut(s) 335
SsiI CCGC 2 cut(s) 172, 833
SspMI CTAG 4 cut(s) 15, 260, 318, 725
StyD4I CCNGG 1 cut(s) 775
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 2 cut(s) 536, 676
TaiI ACGT 3 cut(s) 73, 315, 551
TaqI TCGA 4 cut(s) 364, 495, 960, 1147
TatI WGTACW 2 cut(s) 1043, 1132
TauI GCSGC 1 cut(s) 835
TfiI GAWTC 3 cut(s) 942, 1066, 1102
Tru1I TTAA 7 cut(s) 432, 551, 557, 825, 936, 1050, 1184
Tru9I TTAA 7 cut(s) 432, 551, 557, 825, 936, 1050, 1184
TscAI CASTG 4 cut(s) 124, 331, 344, 444
TseFI GTSAC 1 cut(s) 853
Tsp45I GTSAC 1 cut(s) 853
TspDTI ATGAA 6 cut(s) 53, 132, 215, 494, 584, 1092
TspGWI ACGGA 1 cut(s) 681
TspRI CASTG 4 cut(s) 124, 331, 344, 444
Vha464I CTTAAG 1 cut(s) 1049
VspI ATTAAT 1 cut(s) 825
XapI RAATTY 5 cut(s) 99, 203, 255, 302, 848
XceI RCATGY 2 cut(s) 335, 916
XmiI GTMKAC 1 cut(s) 617
XspI CTAG 4 cut(s) 15, 260, 318, 725
Zsp2I ATGCAT 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.