RLG00000034213

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000912
Physical Location & Seq
Forward (+)
38524727 .. 38526584
1858 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034213

Sequence Viewer

Length: 792 bp
ATGATGGGAAAGTGGTGGAGTATTTCTTCAAATAGAGATGCGAGTTTGCAGAAAGAAATAGCTAAGGTGCTTGAATTTTTCCATTTTGCTTGCACATCTGTGGATATCAACAATCTTGCCCATGCATTAATGCTAGAAGGAGCCATGAACAATGTCGTACTCCCTTTCATGGATGATTTGATTCAGGCTCTATGTAACATGGCCAAGGACAATGCTCATATTCCTAAGGGGTCTCGTACTCATGGCCAGATCTCTTCGGTGGCGATAATGGGGAAATTTTCTGGTGCTGTTGGAAATTACAACACTCATCTTGTTGCATATCCTAATATTAATTGGCCCCAAATAACCAAGGACGGTGAGGTTGGTTCATCAACAATGCCCCAGAAAGTAAACCCTATTGATTTTGAAAACAGCAAAGGTAATCTTGGTGTGGCTATTGCCAATCTTTATCATCCGAGCATGAAAACTTCAGTTTTACTTTCAACTTGTTTATGTTCCCTGCAACGAGACTTGATCGACTCATCTGTTTTGAGGAACATGGATTTAGGATTAAGGCATTCTGTTCTTGCGTACAAAAGCACACTTCAGGGAATATCAAAGCTTCATCTGATGAACAGAGGAATACCAGTTGTCTCGGAAACTATAACAGATTTTATGTGTCTGCTGGACTTACATTACGAACCAAAGATTGAACTACTAGGGTATAGACCGTCCAACACTATTGGAGCAGCTGAAGAATTGGCAAGGAGAATAGACTCGGCTGTGAATGCAAATTTAAGGGAAAAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

264

Amino Acids

29.02

Weight (kDa)

7.11

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_1 PF00206 20 - 102 2.1e-10 Lyase
Lyase_1 PF00206 116 - 142 4.2e-06 Lyase
ASL_C PF08328 167 - 206 2.1e-09 Adenylosuccinate lyase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 201, 244
AcsI RAATTY 3 cut(s) 74, 275, 772
AcuI CTGAAG 3 cut(s) 453, 569, 753
AfaI GTAC 3 cut(s) 159, 238, 572
AfiI CCNNNNNNNGG 1 cut(s) 169
AgsI TTSAA 5 cut(s) 30, 74, 407, 483, 692
AleI CACNNNNGTG 1 cut(s) 98
AluBI AGCT 3 cut(s) 62, 601, 731
AluI AGCT 3 cut(s) 62, 601, 731
Alw26I GTCTC 3 cut(s) 237, 501, 637
AoxI GGCC 3 cut(s) 201, 244, 335
ApeKI GCWGC 1 cut(s) 728
ApoI RAATTY 3 cut(s) 74, 275, 772
AseI ATTAAT 2 cut(s) 128, 330
AspS9I GGNCC 1 cut(s) 336
AsuHPI GGTGA 1 cut(s) 368
AxyI CCTNAGG 1 cut(s) 225
BalI TGGCCA 2 cut(s) 203, 246
BbvI GCAGC 1 cut(s) 740
BcoDI GTCTC 3 cut(s) 237, 501, 637
BfaI CTAG 2 cut(s) 134, 698
BglII AGATCT 1 cut(s) 249
BisI GCNGC 1 cut(s) 729
BlsI GCNGC 1 cut(s) 730
BmgT120I GGNCC 1 cut(s) 336
BmiI GGNNCC 2 cut(s) 142, 338
BmsI GCATC 1 cut(s) 28
Bpu10I CCTNAGC 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 237
BsaJI CCNNGG 2 cut(s) 204, 348
Bsc4I CCNNNNNNNGG 1 cut(s) 169
Bse1I ACTGG 1 cut(s) 626
Bse21I CCTNAGG 1 cut(s) 225
BseDI CCNNGG 2 cut(s) 204, 348
BseGI GGATG 2 cut(s) 178, 451
BseLI CCNNNNNNNGG 1 cut(s) 169
BseNI ACTGG 1 cut(s) 626
BseXI GCAGC 1 cut(s) 740
BshFI GGCC 3 cut(s) 203, 246, 337
BslI CCNNNNNNNGG 1 cut(s) 169
BsmAI GTCTC 3 cut(s) 237, 501, 637
BsmI GAATGC 2 cut(s) 556, 772
BsnI GGCC 3 cut(s) 203, 246, 337
Bso31I GGTCTC 1 cut(s) 237
Bsp143I GATC 2 cut(s) 249, 513
BspANI GGCC 3 cut(s) 203, 246, 337
BspLI GGNNCC 2 cut(s) 142, 338
BspTNI GGTCTC 1 cut(s) 237
BsrI ACTGG 1 cut(s) 626
BssECI CCNNGG 2 cut(s) 204, 348
BssMI GATC 2 cut(s) 249, 513
BssT1I CCWWGG 2 cut(s) 204, 348
Bst4CI ACNGT 2 cut(s) 356, 711
Bst6I CTCTTC 1 cut(s) 259
BstC8I GCNNGC 1 cut(s) 91
BstDEI CTNAG 2 cut(s) 63, 225
BstF5I GGATG 2 cut(s) 178, 451
BstKTI GATC 2 cut(s) 252, 516
BstMAI GTCTC 3 cut(s) 237, 501, 637
BstMBI GATC 2 cut(s) 249, 513
BstMWI GCNNNNNNNGC 1 cut(s) 767
BstV1I GCAGC 1 cut(s) 740
BstX2I RGATCY 1 cut(s) 249
BstYI RGATCY 1 cut(s) 249
Bsu36I CCTNAGG 1 cut(s) 225
BsuRI GGCC 3 cut(s) 203, 246, 337
BtsCI GGATG 2 cut(s) 178, 451
Cac8I GCNNGC 1 cut(s) 91
Cfr13I GGNCC 1 cut(s) 336
Csp6I GTAC 3 cut(s) 158, 237, 571
CviAII CATG 7 cut(s) 122, 145, 169, 199, 242, 460, 538
CviQI GTAC 3 cut(s) 158, 237, 571
DdeI CTNAG 2 cut(s) 63, 225
DpnI GATC 2 cut(s) 251, 515
DpnII GATC 2 cut(s) 249, 513
EaeI YGGCCR 2 cut(s) 201, 244
Eam1104I CTCTTC 1 cut(s) 259
EarI CTCTTC 1 cut(s) 259
Eco130I CCWWGG 2 cut(s) 204, 348
Eco31I GGTCTC 1 cut(s) 237
Eco32I GATATC 1 cut(s) 106
Eco57I CTGAAG 3 cut(s) 453, 569, 753
Eco81I CCTNAGG 1 cut(s) 225
EcoRV GATATC 1 cut(s) 106
EcoT14I CCWWGG 2 cut(s) 204, 348
EcoT22I ATGCAT 1 cut(s) 127
ErhI CCWWGG 2 cut(s) 204, 348
FaeI CATG 7 cut(s) 125, 148, 172, 202, 245, 463, 541
FalI AAGNNNNNCTT 2 cut(s) 408, 440
FatI CATG 7 cut(s) 121, 144, 168, 198, 241, 459, 537
Fnu4HI GCNGC 1 cut(s) 729
FokI GGATG 2 cut(s) 185, 438
Fsp4HI GCNGC 1 cut(s) 729
FspBI CTAG 2 cut(s) 134, 698
GluI GCNGC 1 cut(s) 729
HaeIII GGCC 3 cut(s) 203, 246, 337
Hin1II CATG 7 cut(s) 125, 148, 172, 202, 245, 463, 541
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 3 cut(s) 181, 518, 755
HphI GGTGA 1 cut(s) 368
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 3 cut(s) 456, 609, 637
Hpy8I GTNNAC 1 cut(s) 391
HpyAV CCTTC 1 cut(s) 131
HpyCH4III ACNGT 2 cut(s) 356, 711
HpyCH4V TGCA 6 cut(s) 49, 93, 125, 317, 502, 770
HpyF10VI GCNNNNNNNGC 1 cut(s) 767
HpyF3I CTNAG 2 cut(s) 63, 225
Hsp92II CATG 7 cut(s) 125, 148, 172, 202, 245, 463, 541
Kzo9I GATC 2 cut(s) 249, 513
LmnI GCTCC 2 cut(s) 140, 725
LpnPI CCDG 8 cut(s) 170, 260, 267, 395, 512, 572, 639, 650
Lsp1109I GCAGC 1 cut(s) 740
LweI GCATC 1 cut(s) 28
MaeI CTAG 2 cut(s) 134, 698
MaeIII GTNAC 1 cut(s) 194
MalI GATC 2 cut(s) 251, 515
MboI GATC 2 cut(s) 249, 513
MboII GAAGA 3 cut(s) 18, 246, 746
MflI RGATCY 1 cut(s) 249
MlsI TGGCCA 2 cut(s) 203, 246
MluCI AATT 6 cut(s) 74, 275, 295, 331, 737, 772
MluNI TGGCCA 2 cut(s) 203, 246
MlyI GAGTC 2 cut(s) 512, 749
MmeI TCCRAC 2 cut(s) 271, 738
MnlI CCTC 3 cut(s) 352, 525, 611
Mox20I TGGCCA 2 cut(s) 203, 246
Mph1103I ATGCAT 1 cut(s) 127
MscI TGGCCA 2 cut(s) 203, 246
MseI TTAA 4 cut(s) 128, 330, 551, 776
MslI CAYNNNNRTG 1 cut(s) 98
Msp20I TGGCCA 2 cut(s) 203, 246
MspA1I CMGCKG 1 cut(s) 731
Mva1269I GAATGC 2 cut(s) 556, 772
MwoI GCNNNNNNNGC 1 cut(s) 767
NdeII GATC 2 cut(s) 249, 513
NlaIII CATG 7 cut(s) 125, 148, 172, 202, 245, 463, 541
NlaIV GGNNCC 2 cut(s) 142, 338
NmeAIII GCCGAG 1 cut(s) 737
NsiI ATGCAT 1 cut(s) 127
OliI CACNNNNGTG 1 cut(s) 98
PctI GAATGC 2 cut(s) 556, 772
PfeI GAWTC 1 cut(s) 181
PkrI GCNGC 1 cut(s) 730
PleI GAGTC 2 cut(s) 512, 749
PpsI GAGTC 2 cut(s) 512, 749
PshBI ATTAAT 2 cut(s) 128, 330
PspN4I GGNNCC 2 cut(s) 142, 338
PspPI GGNCC 1 cut(s) 336
PsuI RGATCY 1 cut(s) 249
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 3 cut(s) 159, 238, 572
RsaNI GTAC 3 cut(s) 158, 237, 571
RseI CAYNNNNRTG 1 cut(s) 98
SaqAI TTAA 4 cut(s) 128, 330, 551, 776
SatI GCNGC 1 cut(s) 729
Sau3AI GATC 2 cut(s) 249, 513
Sau96I GGNCC 1 cut(s) 336
SchI GAGTC 2 cut(s) 512, 749
SetI ASST 6 cut(s) 64, 69, 363, 421, 603, 733
SfaNI GCATC 1 cut(s) 28
SmiMI CAYNNNNRTG 1 cut(s) 98
Sse9I AATT 6 cut(s) 74, 275, 295, 331, 737, 772
SspI AATATT 1 cut(s) 328
SspMI CTAG 2 cut(s) 134, 698
StyI CCWWGG 2 cut(s) 204, 348
TaaI ACNGT 2 cut(s) 356, 711
TaqI TCGA 1 cut(s) 516
TasI AATT 6 cut(s) 74, 275, 295, 331, 737, 772
TfiI GAWTC 1 cut(s) 181
Tru1I TTAA 4 cut(s) 128, 330, 551, 776
Tru9I TTAA 4 cut(s) 128, 330, 551, 776
TseI GCWGC 1 cut(s) 728
TspDTI ATGAA 6 cut(s) 157, 161, 357, 476, 593, 626
VspI ATTAAT 2 cut(s) 128, 330
XapI RAATTY 3 cut(s) 74, 275, 772
XspI CTAG 2 cut(s) 134, 698
Zsp2I ATGCAT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.