Rroxscaffold_1G00037270

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000912
Physical Location & Seq
Reverse (-)
54338233 .. 54342132
3900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00037270.1

Sequence Viewer

Length: 276 bp
ATGGCAACTTTTGTTGTGAGATTAAGGAGAGAAAGGAAAGAGATCTCTTCAGTGGCGATAATAGGGAAATTTTCTGGTGCTGTTGGAAATTACAACACTCATCTTGTTGCATATCCTAATCTTAATTGGCCCCACAAGTTGCTTAAGACCTTAGGCTGGTCCTCCGATAACCTTCCATCTCTTCACATTTGTAGGTTTTTCCATGGAGCTCCGCAAACTCGAGGAGCTGGATGCCCAACGTCGGGTGAAGGATATTGTATGCCCAAGCCGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

91

Amino Acids

10.07

Weight (kDa)

9.66

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 212
AcsI RAATTY 1 cut(s) 68
AcuI CTGAAG 1 cut(s) 33
AfiI CCNNNNNNNGG 3 cut(s) 156, 241, 242
AflII CTTAAG 1 cut(s) 143
AluBI AGCT 2 cut(s) 209, 227
AluI AGCT 2 cut(s) 209, 227
Alw21I GWGCWC 1 cut(s) 211
Ama87I CYCGRG 1 cut(s) 219
AoxI GGCC 1 cut(s) 128
ApoI RAATTY 1 cut(s) 68
AspS9I GGNCC 2 cut(s) 129, 159
AsuHPI GGTGA 1 cut(s) 257
AvaI CYCGRG 1 cut(s) 219
AvaII GGWCC 1 cut(s) 159
AxyI CCTNAGG 1 cut(s) 151
BanII GRGCYC 1 cut(s) 211
Bbv12I GWGCWC 1 cut(s) 211
BccI CCATC 1 cut(s) 184
BfaI CTAG 1 cut(s) 274
BfrI CTTAAG 1 cut(s) 143
BglII AGATCT 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 159
BmeT110I CYCGRG 1 cut(s) 219
BmgT120I GGNCC 2 cut(s) 129, 159
BmiI GGNNCC 1 cut(s) 131
BmsI GCATC 1 cut(s) 221
BsaJI CCNNGG 1 cut(s) 202
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 241, 242
Bse21I CCTNAGG 1 cut(s) 151
BseDI CCNNGG 1 cut(s) 202
BseGI GGATG 1 cut(s) 236
BseLI CCNNNNNNNGG 3 cut(s) 156, 241, 242
BseRI GAGGAG 1 cut(s) 237
BshFI GGCC 1 cut(s) 130
BsiHKAI GWGCWC 1 cut(s) 211
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 1 cut(s) 269
BslI CCNNNNNNNGG 3 cut(s) 156, 241, 242
BsnI GGCC 1 cut(s) 130
BsoBI CYCGRG 1 cut(s) 219
Bsp1286I GDGCHC 1 cut(s) 211
Bsp143I GATC 1 cut(s) 42
Bsp19I CCATGG 1 cut(s) 202
BspACI CCGC 1 cut(s) 212
BspANI GGCC 1 cut(s) 130
BspLI GGNNCC 1 cut(s) 131
BspTI CTTAAG 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 202
BssMI GATC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 202
Bst6I CTCTTC 2 cut(s) 52, 186
BstAFI CTTAAG 1 cut(s) 143
BstDEI CTNAG 1 cut(s) 151
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 1 cut(s) 236
BstKTI GATC 1 cut(s) 45
BstMBI GATC 1 cut(s) 42
BstX2I RGATCY 1 cut(s) 42
BstYI RGATCY 1 cut(s) 42
Bsu36I CCTNAGG 1 cut(s) 151
BsuRI GGCC 1 cut(s) 130
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 1 cut(s) 236
BtsIMutI CAGTG 1 cut(s) 57
Cfr13I GGNCC 2 cut(s) 129, 159
CviAII CATG 1 cut(s) 203
CviJI RGCY 5 cut(s) 130, 156, 209, 227, 268
CviKI_1 RGCY 5 cut(s) 130, 156, 209, 227, 268
DdeI CTNAG 1 cut(s) 151
DpnI GATC 1 cut(s) 44
DpnII GATC 1 cut(s) 42
Eam1104I CTCTTC 2 cut(s) 52, 186
EarI CTCTTC 2 cut(s) 52, 186
Ecl136II GAGCTC 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 202
Eco24I GRGCYC 1 cut(s) 211
Eco47I GGWCC 1 cut(s) 159
Eco53kI GAGCTC 1 cut(s) 209
Eco57I CTGAAG 1 cut(s) 33
Eco81I CCTNAGG 1 cut(s) 151
Eco88I CYCGRG 1 cut(s) 219
EcoICRI GAGCTC 1 cut(s) 209
EcoT14I CCWWGG 1 cut(s) 202
EcoT38I GRGCYC 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 202
FaeI CATG 1 cut(s) 206
FaiI YATR 3 cut(s) 112, 204, 260
FatI CATG 1 cut(s) 202
FokI GGATG 1 cut(s) 243
FriOI GRGCYC 1 cut(s) 211
FspBI CTAG 1 cut(s) 274
HaeIII GGCC 1 cut(s) 130
HapII CCGG 1 cut(s) 269
Hin1II CATG 1 cut(s) 206
HpaII CCGG 1 cut(s) 269
HphI GGTGA 1 cut(s) 257
Hpy188I TCNGA 1 cut(s) 166
Hpy99I CGWCG 1 cut(s) 244
HpyAV CCTTC 2 cut(s) 182, 242
HpyCH4IV ACGT 1 cut(s) 239
HpyCH4V TGCA 1 cut(s) 110
HpyF3I CTNAG 1 cut(s) 151
HpySE526I ACGT 1 cut(s) 239
Hsp92II CATG 1 cut(s) 206
Kzo9I GATC 1 cut(s) 42
LmnI GCTCC 3 cut(s) 206, 214, 224
LpnPI CCDG 3 cut(s) 60, 142, 213
LweI GCATC 1 cut(s) 221
MaeI CTAG 1 cut(s) 274
MaeII ACGT 1 cut(s) 239
MalI GATC 1 cut(s) 44
MboI GATC 1 cut(s) 42
MboII GAAGA 2 cut(s) 39, 173
MflI RGATCY 1 cut(s) 42
MhlI GDGCHC 1 cut(s) 211
MluCI AATT 3 cut(s) 68, 88, 124
MmeI TCCRAC 1 cut(s) 64
MnlI CCTC 2 cut(s) 172, 215
MseI TTAA 3 cut(s) 23, 123, 144
MspCI CTTAAG 1 cut(s) 143
MspI CCGG 1 cut(s) 269
NcoI CCATGG 1 cut(s) 202
NdeII GATC 1 cut(s) 42
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 1 cut(s) 131
PaeR7I CTCGAG 1 cut(s) 219
Psp124BI GAGCTC 1 cut(s) 211
PspN4I GGNNCC 1 cut(s) 131
PspPI GGNCC 2 cut(s) 129, 159
PspXI VCTCGAGB 1 cut(s) 219
PsuI RGATCY 1 cut(s) 42
SacI GAGCTC 1 cut(s) 211
SaqAI TTAA 3 cut(s) 23, 123, 144
Sau3AI GATC 1 cut(s) 42
Sau96I GGNCC 2 cut(s) 129, 159
SduI GDGCHC 1 cut(s) 211
SetI ASST 6 cut(s) 152, 174, 197, 211, 229, 242
SfaNI GCATC 1 cut(s) 221
Sfr274I CTCGAG 1 cut(s) 219
SgeI CNNG 9 cut(s) 87, 116, 148, 169, 215, 231, 233, 240, 255
SinI GGWCC 1 cut(s) 159
SlaI CTCGAG 1 cut(s) 219
SmlI CTYRAG 2 cut(s) 143, 219
SmoI CTYRAG 2 cut(s) 143, 219
Sse9I AATT 3 cut(s) 68, 88, 124
SsiI CCGC 1 cut(s) 212
SspMI CTAG 1 cut(s) 274
SstI GAGCTC 1 cut(s) 211
StyI CCWWGG 1 cut(s) 202
TaiI ACGT 1 cut(s) 242
TaqI TCGA 1 cut(s) 220
TasI AATT 3 cut(s) 68, 88, 124
Tru1I TTAA 3 cut(s) 23, 123, 144
Tru9I TTAA 3 cut(s) 23, 123, 144
TscAI CASTG 1 cut(s) 57
TspRI CASTG 1 cut(s) 57
Vha464I CTTAAG 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 159
XapI RAATTY 1 cut(s) 68
XhoI CTCGAG 1 cut(s) 219
XspI CTAG 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.