RchiOBHm_Chr5g0043801

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000912
Physical Location & Seq
Forward (+)
38867893 .. 38869271
1379 bp
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UTR
Exon/CDS
Intron
PRQ32203

Sequence Viewer

Length: 723 bp
ATGCGAAACAAAATGTTCCTTGTGCTTGAATTTTTCCATTTTGCTTGCACATCTGAGGATATCAACAATCTTGCCCATGCATTAATGCTAGAAGGAGCCATGAACAATGTCGTAATCCATTTCATGGATGATTTGATTCAGGCACTGTGTAACATGGCTAAGGACAATGCTCATATTCCTAGGGGGTCTTGTACTCATGGCCAGATCTCTTCGGTGGCGATAATGGGGAAATTTTCTGGTGCTGTTGGAAATTACAACACTCATCTTGTTGCATATCCTAATATTAATTGGCCCCAAATAAATAAGGACGGTCAGGTTGGTTCATCAACAATGCCTCAGAAAATAAACCCTATTGATTTTGAAAATAGCAAAGGCAATCTTAGTGTGGCTAATGCCAATCTTTATCACCCGAGCATGAAGTTGCCTATTTCACGTTTGCAGCTAAAAAGAACTTCAGTCATCCTTTCAAGTTGTTTCTGTTCCCTGCAACGAGACTTGATCGACTTATCTGTTTTGAGGAACATGGATTTAGGATTAGGGCATTCTCTTCTTGCCTACAAAAGCACACTTCAGGGAATATCAAAGGCTCAAAGCTTCAGGTATGCAGCACGTACAGTATATAACTTTGCAATAAATGAAGGTTGCTTGCTAATATTCTTTGAGGTCAAAATTAGATACAAGCCAAAAAGAGAATACACTTTCTGCACTAGGATATATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

240

Amino Acids

27.06

Weight (kDa)

9.17

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_1 PF00206 10 - 87 7.7e-08 Lyase
ASL_C PF08328 162 - 197 1.7e-07 Adenylosuccinate lyase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 124
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 2 cut(s) 29, 230
AcuI CTGAAG 3 cut(s) 438, 554, 580
AfaI GTAC 2 cut(s) 193, 613
AfiI CCNNNNNNNGG 1 cut(s) 124
AgsI TTSAA 3 cut(s) 29, 362, 468
AluBI AGCT 2 cut(s) 442, 594
AluI AGCT 2 cut(s) 442, 594
Alw26I GTCTC 1 cut(s) 486
AlwNI CAGNNNCTG 1 cut(s) 145
Ama87I CYCGRG 1 cut(s) 409
AoxI GGCC 2 cut(s) 199, 290
ApeKI GCWGC 2 cut(s) 439, 605
ApoI RAATTY 2 cut(s) 29, 230
AseI ATTAAT 2 cut(s) 83, 285
AspA2I CCTAGG 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 291
AsuHPI GGTGA 1 cut(s) 398
AvaI CYCGRG 1 cut(s) 409
AvrII CCTAGG 1 cut(s) 179
BalI TGGCCA 1 cut(s) 201
BbvI GCAGC 2 cut(s) 451, 617
BcoDI GTCTC 1 cut(s) 486
BfaI CTAG 3 cut(s) 89, 180, 708
BglII AGATCT 1 cut(s) 204
BisI GCNGC 2 cut(s) 440, 606
BlnI CCTAGG 1 cut(s) 179
BlsI GCNGC 2 cut(s) 441, 607
BmeT110I CYCGRG 1 cut(s) 409
BmgT120I GGNCC 1 cut(s) 291
BmiI GGNNCC 2 cut(s) 97, 293
Bpu10I CCTNAGC 1 cut(s) 159
BsaAI YACGTR 1 cut(s) 611
BsaJI CCNNGG 1 cut(s) 179
Bsc4I CCNNNNNNNGG 1 cut(s) 124
BseDI CCNNGG 1 cut(s) 179
BseGI GGATG 2 cut(s) 133, 459
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMII CTCAG 2 cut(s) 45, 350
BseXI GCAGC 2 cut(s) 451, 617
BsgI GTGCAG 1 cut(s) 688
BshFI GGCC 2 cut(s) 201, 292
BsiHKCI CYCGRG 1 cut(s) 409
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 1 cut(s) 486
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 2 cut(s) 201, 292
BsoBI CYCGRG 1 cut(s) 409
Bsp143I GATC 2 cut(s) 204, 498
BspANI GGCC 2 cut(s) 201, 292
BspCNI CTCAG 2 cut(s) 46, 349
BspLI GGNNCC 2 cut(s) 97, 293
BssECI CCNNGG 1 cut(s) 179
BssMI GATC 2 cut(s) 204, 498
BssT1I CCWWGG 1 cut(s) 179
Bst4CI ACNGT 3 cut(s) 147, 311, 616
Bst6I CTCTTC 2 cut(s) 214, 552
BstBAI YACGTR 1 cut(s) 611
BstC8I GCNNGC 2 cut(s) 46, 647
BstDEI CTNAG 4 cut(s) 54, 159, 336, 380
BstF5I GGATG 2 cut(s) 133, 459
BstKTI GATC 2 cut(s) 207, 501
BstMAI GTCTC 1 cut(s) 486
BstMBI GATC 2 cut(s) 204, 498
BstV1I GCAGC 2 cut(s) 451, 617
BstX2I RGATCY 1 cut(s) 204
BstYI RGATCY 1 cut(s) 204
BsuRI GGCC 2 cut(s) 201, 292
BtsCI GGATG 2 cut(s) 133, 459
BtsIMutI CAGTG 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 46, 647
CaiI CAGNNNCTG 1 cut(s) 145
Cfr13I GGNCC 1 cut(s) 291
Csp6I GTAC 2 cut(s) 192, 612
CviAII CATG 7 cut(s) 77, 100, 124, 154, 197, 415, 523
CviJI RGCY 9 cut(s) 98, 158, 201, 292, 389, 442, 587, 594, 682
CviKI_1 RGCY 9 cut(s) 98, 158, 201, 292, 389, 442, 587, 594, 682
CviQI GTAC 2 cut(s) 192, 612
DdeI CTNAG 4 cut(s) 54, 159, 336, 380
DpnI GATC 2 cut(s) 206, 500
DpnII GATC 2 cut(s) 204, 498
EaeI YGGCCR 1 cut(s) 199
Eam1104I CTCTTC 2 cut(s) 214, 552
EarI CTCTTC 2 cut(s) 214, 552
Eco130I CCWWGG 1 cut(s) 179
Eco32I GATATC 1 cut(s) 61
Eco57I CTGAAG 3 cut(s) 438, 554, 580
Eco88I CYCGRG 1 cut(s) 409
EcoRV GATATC 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 179
EcoT22I ATGCAT 1 cut(s) 82
ErhI CCWWGG 1 cut(s) 179
FaeI CATG 7 cut(s) 80, 103, 127, 157, 200, 418, 526
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FatI CATG 7 cut(s) 76, 99, 123, 153, 196, 414, 522
Fnu4HI GCNGC 2 cut(s) 440, 606
FokI GGATG 2 cut(s) 140, 446
Fsp4HI GCNGC 2 cut(s) 440, 606
FspBI CTAG 3 cut(s) 89, 180, 708
GluI GCNGC 2 cut(s) 440, 606
HaeIII GGCC 2 cut(s) 201, 292
Hin1II CATG 7 cut(s) 80, 103, 127, 157, 200, 418, 526
HindIII AAGCTT 1 cut(s) 592
HinfI GANTC 1 cut(s) 136
HphI GGTGA 1 cut(s) 398
Hpy188I TCNGA 3 cut(s) 55, 339, 722
HpyAV CCTTC 2 cut(s) 86, 632
HpyCH4III ACNGT 3 cut(s) 147, 311, 616
HpyCH4IV ACGT 2 cut(s) 433, 610
HpyCH4V TGCA 8 cut(s) 48, 80, 272, 439, 487, 605, 629, 705
HpyF3I CTNAG 4 cut(s) 54, 159, 336, 380
HpySE526I ACGT 2 cut(s) 433, 610
Hsp92II CATG 7 cut(s) 80, 103, 127, 157, 200, 418, 526
Kzo9I GATC 2 cut(s) 204, 498
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 7 cut(s) 125, 215, 222, 299, 497, 557, 583
Lsp1109I GCAGC 2 cut(s) 451, 617
MaeI CTAG 3 cut(s) 89, 180, 708
MaeII ACGT 2 cut(s) 433, 610
MaeIII GTNAC 1 cut(s) 149
MalI GATC 2 cut(s) 206, 500
MboI GATC 2 cut(s) 204, 498
MboII GAAGA 2 cut(s) 201, 539
MflI RGATCY 1 cut(s) 204
MlsI TGGCCA 1 cut(s) 201
MluCI AATT 5 cut(s) 29, 230, 250, 286, 669
MluNI TGGCCA 1 cut(s) 201
MmeI TCCRAC 1 cut(s) 226
MnlI CCTC 4 cut(s) 49, 345, 510, 655
Mox20I TGGCCA 1 cut(s) 201
Mph1103I ATGCAT 1 cut(s) 82
MscI TGGCCA 1 cut(s) 201
MseI TTAA 2 cut(s) 83, 285
Msp20I TGGCCA 1 cut(s) 201
Mva1269I GAATGC 1 cut(s) 541
NdeII GATC 2 cut(s) 204, 498
NlaIII CATG 7 cut(s) 80, 103, 127, 157, 200, 418, 526
NlaIV GGNNCC 2 cut(s) 97, 293
NsiI ATGCAT 1 cut(s) 82
PctI GAATGC 1 cut(s) 541
PfeI GAWTC 1 cut(s) 136
PflMI CCANNNNNTGG 1 cut(s) 124
PkrI GCNGC 2 cut(s) 441, 607
Ppu21I YACGTR 1 cut(s) 611
PshBI ATTAAT 2 cut(s) 83, 285
PspN4I GGNNCC 2 cut(s) 97, 293
PspPI GGNCC 1 cut(s) 291
PstNI CAGNNNCTG 1 cut(s) 145
PsuI RGATCY 1 cut(s) 204
RsaI GTAC 2 cut(s) 193, 613
RsaNI GTAC 2 cut(s) 192, 612
SaqAI TTAA 2 cut(s) 83, 285
SatI GCNGC 2 cut(s) 440, 606
Sau3AI GATC 2 cut(s) 204, 498
Sau96I GGNCC 1 cut(s) 291
SetI ASST 8 cut(s) 318, 436, 444, 596, 602, 613, 643, 666
Sse9I AATT 5 cut(s) 29, 230, 250, 286, 669
SspI AATATT 2 cut(s) 283, 654
SspMI CTAG 3 cut(s) 89, 180, 708
StyI CCWWGG 1 cut(s) 179
TaaI ACNGT 3 cut(s) 147, 311, 616
TaiI ACGT 2 cut(s) 436, 613
TaqI TCGA 1 cut(s) 501
TasI AATT 5 cut(s) 29, 230, 250, 286, 669
TatI WGTACW 1 cut(s) 191
TfiI GAWTC 1 cut(s) 136
Tru1I TTAA 2 cut(s) 83, 285
Tru9I TTAA 2 cut(s) 83, 285
TscAI CASTG 1 cut(s) 150
TseI GCWGC 2 cut(s) 439, 605
TspDTI ATGAA 5 cut(s) 112, 116, 312, 431, 651
TspRI CASTG 1 cut(s) 150
Van91I CCANNNNNTGG 1 cut(s) 124
VspI ATTAAT 2 cut(s) 83, 285
XapI RAATTY 2 cut(s) 29, 230
XmaJI CCTAGG 1 cut(s) 179
XspI CTAG 3 cut(s) 89, 180, 708
Zsp2I ATGCAT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.