Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 218, 250 |
| AccB1I | GGYRCC | 1 cut(s) | 391 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1712 |
| AccI | GTMKAC | 1 cut(s) | 3039 |
| AccIII | TCCGGA | 1 cut(s) | 235 |
| AclWI | GGATC | 3 cut(s) | 1570, 2817, 3075 |
| AcoI | YGGCCR | 5 cut(s) | 265, 1276, 1596, 1714, 3027 |
| AcsI | RAATTY | 4 cut(s) | 397, 1821, 2018, 3052 |
| AfaI | GTAC | 8 cut(s) | 14, 518, 733, 1058, 1446, 2140, 2156, 2752 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 263, 274, 1712, 1723, 1851, 2114 |
| AjnI | CCWGG | 1 cut(s) | 3029 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1149, 1181, 2598, 2630 |
| Alw21I | GWGCWC | 2 cut(s) | 8, 1864 |
| Alw26I | GTCTC | 4 cut(s) | 68, 258, 965, 2274 |
| AlwI | GGATC | 3 cut(s) | 1570, 2817, 3075 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 638, 1469 |
| Ama87I | CYCGRG | 1 cut(s) | 1676 |
| Aor13HI | TCCGGA | 1 cut(s) | 235 |
| ApeKI | GCWGC | 5 cut(s) | 710, 1225, 1306, 2674, 3014 |
| ApoI | RAATTY | 4 cut(s) | 397, 1821, 2018, 3052 |
| AseI | ATTAAT | 1 cut(s) | 453 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 538, 2022, 2445 |
| AspLEI | GCGC | 1 cut(s) | 3014 |
| AspS9I | GGNCC | 3 cut(s) | 189, 903, 2352 |
| AsuHPI | GGTGA | 6 cut(s) | 744, 1109, 1654, 2129, 2193, 2558 |
| AvaI | CYCGRG | 1 cut(s) | 1676 |
| BalI | TGGCCA | 2 cut(s) | 1278, 3029 |
| BanI | GGYRCC | 1 cut(s) | 391 |
| BanII | GRGCYC | 2 cut(s) | 8, 2877 |
| BauI | CACGAG | 1 cut(s) | 2271 |
| BbsI | GAAGAC | 3 cut(s) | 491, 1101, 2550 |
| Bbv12I | GWGCWC | 2 cut(s) | 8, 1864 |
| BbvI | GCAGC | 5 cut(s) | 697, 1237, 1293, 2686, 3026 |
| BccI | CCATC | 7 cut(s) | 32, 434, 1200, 1642, 1706, 1883, 3028 |
| BceAI | ACGGC | 6 cut(s) | 223, 252, 261, 280, 762, 1583 |
| BciT130I | CCWGG | 1 cut(s) | 3031 |
| BciVI | GTATCC | 1 cut(s) | 1064 |
| BcoDI | GTCTC | 4 cut(s) | 68, 258, 965, 2274 |
| BfaI | CTAG | 2 cut(s) | 1176, 1913 |
| BfmI | CTRYAG | 3 cut(s) | 855, 1294, 1470 |
| BfuI | GTATCC | 1 cut(s) | 1064 |
| BglII | AGATCT | 1 cut(s) | 2378 |
| BisI | GCNGC | 8 cut(s) | 192, 195, 198, 711, 1226, 1307, 2675, 3015 |
| BlsI | GCNGC | 8 cut(s) | 193, 196, 199, 712, 1227, 1308, 2676, 3016 |
| BmcAI | AGTACT | 2 cut(s) | 518, 2156 |
| Bme1390I | CCNGG | 1 cut(s) | 3031 |
| BmeT110I | CYCGRG | 1 cut(s) | 1676 |
| BmgT120I | GGNCC | 3 cut(s) | 189, 903, 2352 |
| BmiI | GGNNCC | 4 cut(s) | 297, 393, 1746, 2354 |
| BmrFI | CCNGG | 1 cut(s) | 3031 |
| BmsI | GCATC | 5 cut(s) | 241, 448, 1908, 1973, 2064 |
| BpiI | GAAGAC | 3 cut(s) | 491, 1101, 2550 |
| Bpu10I | CCTNAGC | 3 cut(s) | 558, 2148, 2871 |
| BpuEI | CTTGAG | 2 cut(s) | 587, 807 |
| BsaAI | YACGTR | 1 cut(s) | 731 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1359, 1987 |
| BsaI | GGTCTC | 1 cut(s) | 68 |
| BsaJI | CCNNGG | 3 cut(s) | 2043, 2142, 2919 |
| BsaWI | WCCGGW | 2 cut(s) | 235, 2460 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2848, 2878 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 263, 274, 1712, 1723, 1851, 2114 |
| Bse118I | RCCGGY | 1 cut(s) | 1716 |
| Bse1I | ACTGG | 2 cut(s) | 2298, 2548 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1359, 1987 |
| BseAI | TCCGGA | 1 cut(s) | 235 |
| BseBI | CCWGG | 1 cut(s) | 3031 |
| BseDI | CCNNGG | 3 cut(s) | 2043, 2142, 2919 |
| BseGI | GGATG | 7 cut(s) | 24, 148, 232, 544, 1717, 2039, 3039 |
| BseJI | GATNNNNATC | 2 cut(s) | 1359, 1987 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 263, 274, 1712, 1723, 1851, 2114 |
| BseMII | CTCAG | 9 cut(s) | 199, 428, 585, 1187, 1244, 1626, 1877, 2426, 2862 |
| BseNI | ACTGG | 2 cut(s) | 2298, 2548 |
| BseRI | GAGGAG | 3 cut(s) | 81, 156, 2869 |
| BseX3I | CGGCCG | 1 cut(s) | 265 |
| BseXI | GCAGC | 5 cut(s) | 697, 1237, 1293, 2686, 3026 |
| Bsh1285I | CGRYCG | 1 cut(s) | 268 |
| BshNI | GGYRCC | 1 cut(s) | 391 |
| BsiEI | CGRYCG | 1 cut(s) | 268 |
| BsiHKAI | GWGCWC | 2 cut(s) | 8, 1864 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1676 |
| BsiSI | CCGG | 3 cut(s) | 236, 1717, 2461 |
| BslFI | GGGAC | 2 cut(s) | 2516, 2693 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 263, 274, 1712, 1723, 1851, 2114 |
| BsmAI | GTCTC | 4 cut(s) | 68, 258, 965, 2274 |
| BsmFI | GGGAC | 2 cut(s) | 2516, 2693 |
| BsmI | GAATGC | 1 cut(s) | 2728 |
| Bso31I | GGTCTC | 1 cut(s) | 68 |
| BsoBI | CYCGRG | 1 cut(s) | 1676 |
| Bsp1286I | GDGCHC | 3 cut(s) | 8, 1864, 2877 |
| Bsp13I | TCCGGA | 1 cut(s) | 235 |
| Bsp143I | GATC | 6 cut(s) | 1029, 1562, 1794, 2378, 2809, 3067 |
| Bsp19I | CCATGG | 1 cut(s) | 2142 |
| BspCNI | CTCAG | 9 cut(s) | 198, 427, 586, 1188, 1245, 1627, 1876, 2425, 2863 |
| BspEI | TCCGGA | 1 cut(s) | 235 |
| BspHI | TCATGA | 3 cut(s) | 51, 2323, 2425 |
| BspLI | GGNNCC | 4 cut(s) | 297, 393, 1746, 2354 |
| BspMAI | CTGCAG | 1 cut(s) | 1474 |
| BspPI | GGATC | 3 cut(s) | 1570, 2817, 3075 |
| BspT107I | GGYRCC | 1 cut(s) | 391 |
| BspTNI | GGTCTC | 1 cut(s) | 68 |
| BsrFI | RCCGGY | 1 cut(s) | 1716 |
| BsrI | ACTGG | 2 cut(s) | 2298, 2548 |
| BssAI | RCCGGY | 1 cut(s) | 1716 |
| BssECI | CCNNGG | 3 cut(s) | 2043, 2142, 2919 |
| BssMI | GATC | 6 cut(s) | 1029, 1562, 1794, 2378, 2809, 3067 |
| BssSI | CACGAG | 1 cut(s) | 2271 |
| BssT1I | CCWWGG | 2 cut(s) | 2142, 2919 |
| Bst2BI | CACGAG | 1 cut(s) | 2271 |
| Bst2UI | CCWGG | 1 cut(s) | 3031 |
| Bst6I | CTCTTC | 5 cut(s) | 73, 459, 912, 1290, 2739 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 177 |
| BstBAI | YACGTR | 1 cut(s) | 731 |
| BstC8I | GCNNGC | 9 cut(s) | 586, 708, 846, 1304, 1380, 1695, 2035, 2196, 2791 |
| BstDSI | CCRYGG | 1 cut(s) | 2142 |
| BstEII | GGTNACC | 1 cut(s) | 1840 |
| BstF5I | GGATG | 7 cut(s) | 24, 148, 232, 544, 1717, 2039, 3039 |
| BstHHI | GCGC | 1 cut(s) | 3014 |
| BstKTI | GATC | 6 cut(s) | 1032, 1565, 1797, 2381, 2812, 3070 |
| BstMAI | GTCTC | 4 cut(s) | 68, 258, 965, 2274 |
| BstMBI | GATC | 6 cut(s) | 1029, 1562, 1794, 2378, 2809, 3067 |
| BstMCI | CGRYCG | 1 cut(s) | 268 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 177, 197, 785, 1604, 2234 |
| BstNI | CCWGG | 1 cut(s) | 3031 |
| BstNSI | RCATGY | 3 cut(s) | 710, 2037, 2069 |
| BstPI | GGTNACC | 1 cut(s) | 1840 |
| BstSCI | CCNGG | 1 cut(s) | 3029 |
| BstSFI | CTRYAG | 3 cut(s) | 855, 1294, 1470 |
| BstV1I | GCAGC | 5 cut(s) | 697, 1237, 1293, 2686, 3026 |
| BstV2I | GAAGAC | 3 cut(s) | 491, 1101, 2550 |
| BstX2I | RGATCY | 3 cut(s) | 1562, 2378, 3067 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2950 |
| BstYI | RGATCY | 3 cut(s) | 1562, 2378, 3067 |
| BstZI | CGGCCG | 1 cut(s) | 265 |
| BsuI | GTATCC | 1 cut(s) | 1064 |
| BtgI | CCRYGG | 1 cut(s) | 2142 |
| BtsCI | GGATG | 7 cut(s) | 24, 148, 232, 544, 1717, 2039, 3039 |
| BtsIMutI | CAGTG | 2 cut(s) | 2608, 2819 |
| Cac8I | GCNNGC | 9 cut(s) | 586, 708, 846, 1304, 1380, 1695, 2035, 2196, 2791 |
| CaiI | CAGNNNCTG | 2 cut(s) | 638, 1469 |
| CciI | TCATGA | 3 cut(s) | 51, 2323, 2425 |
| CfoI | GCGC | 1 cut(s) | 3014 |
| Cfr10I | RCCGGY | 1 cut(s) | 1716 |
| Cfr13I | GGNCC | 3 cut(s) | 189, 903, 2352 |
| CseI | GACGC | 1 cut(s) | 1916 |
| Csp6I | GTAC | 8 cut(s) | 13, 517, 732, 1057, 1445, 2139, 2155, 2751 |
| CviQI | GTAC | 8 cut(s) | 13, 517, 732, 1057, 1445, 2139, 2155, 2751 |
| DpnI | GATC | 6 cut(s) | 1031, 1564, 1796, 2380, 2811, 3069 |
| DpnII | GATC | 6 cut(s) | 1029, 1562, 1794, 2378, 2809, 3067 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 218, 250 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 218, 250 |
| EaeI | YGGCCR | 5 cut(s) | 265, 1276, 1596, 1714, 3027 |
| EagI | CGGCCG | 1 cut(s) | 265 |
| Eam1104I | CTCTTC | 5 cut(s) | 73, 459, 912, 1290, 2739 |
| EarI | CTCTTC | 5 cut(s) | 73, 459, 912, 1290, 2739 |
| EciI | GGCGGA | 1 cut(s) | 2453 |
| Ecl136II | GAGCTC | 1 cut(s) | 6 |
| EclXI | CGGCCG | 1 cut(s) | 265 |
| Eco130I | CCWWGG | 2 cut(s) | 2142, 2919 |
| Eco24I | GRGCYC | 2 cut(s) | 8, 2877 |
| Eco31I | GGTCTC | 1 cut(s) | 68 |
| Eco32I | GATATC | 2 cut(s) | 601, 2766 |
| Eco52I | CGGCCG | 1 cut(s) | 265 |
| Eco53kI | GAGCTC | 1 cut(s) | 6 |
| Eco88I | CYCGRG | 1 cut(s) | 1676 |
| Eco91I | GGTNACC | 1 cut(s) | 1840 |
| EcoICRI | GAGCTC | 1 cut(s) | 6 |
| EcoO65I | GGTNACC | 1 cut(s) | 1840 |
| EcoRI | GAATTC | 1 cut(s) | 3052 |
| EcoRII | CCWGG | 1 cut(s) | 3029 |
| EcoRV | GATATC | 2 cut(s) | 601, 2766 |
| EcoT14I | CCWWGG | 2 cut(s) | 2142, 2919 |
| EcoT22I | ATGCAT | 1 cut(s) | 2071 |
| EcoT38I | GRGCYC | 2 cut(s) | 8, 2877 |
| ErhI | CCWWGG | 2 cut(s) | 2142, 2919 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1149, 1181, 2598, 2630 |
| FaqI | GGGAC | 2 cut(s) | 2516, 2693 |
| FauI | CCCGC | 3 cut(s) | 80, 1302, 2670 |
| FauNDI | CATATG | 3 cut(s) | 876, 955, 1429 |
| FblI | GTMKAC | 1 cut(s) | 3039 |
| Fnu4HI | GCNGC | 8 cut(s) | 192, 195, 198, 711, 1226, 1307, 2675, 3015 |
| FokI | GGATG | 7 cut(s) | 11, 135, 219, 531, 1724, 2026, 3046 |
| FriOI | GRGCYC | 2 cut(s) | 8, 2877 |
| Fsp4HI | GCNGC | 8 cut(s) | 192, 195, 198, 711, 1226, 1307, 2675, 3015 |
| FspBI | CTAG | 2 cut(s) | 1176, 1913 |
| GlaI | GCGC | 1 cut(s) | 3013 |
| GluI | GCNGC | 8 cut(s) | 192, 195, 198, 711, 1226, 1307, 2675, 3015 |
| HapII | CCGG | 3 cut(s) | 236, 1717, 2461 |
| HgaI | GACGC | 1 cut(s) | 1916 |
| HhaI | GCGC | 1 cut(s) | 3014 |
| Hin6I | GCGC | 1 cut(s) | 3012 |
| HinP1I | GCGC | 1 cut(s) | 3012 |
| HincII | GTYRAC | 3 cut(s) | 630, 643, 2581 |
| HindII | GTYRAC | 3 cut(s) | 630, 643, 2581 |
| HindIII | AAGCTT | 5 cut(s) | 130, 362, 842, 1322, 2787 |
| HinfI | GANTC | 7 cut(s) | 48, 635, 676, 1014, 1241, 1360, 2690 |
| HpaII | CCGG | 3 cut(s) | 236, 1717, 2461 |
| HphI | GGTGA | 6 cut(s) | 744, 1109, 1654, 2129, 2193, 2558 |
| Hpy188I | TCNGA | 8 cut(s) | 595, 1013, 1197, 1254, 2044, 2243, 2415, 3058 |
| HpyCH4IV | ACGT | 4 cut(s) | 730, 1219, 1950, 2668 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 177, 197, 785, 1604, 2234 |
| HpySE526I | ACGT | 4 cut(s) | 730, 1219, 1950, 2668 |
| HspAI | GCGC | 1 cut(s) | 3012 |
| Kpn2I | TCCGGA | 1 cut(s) | 235 |
| Kzo9I | GATC | 6 cut(s) | 1029, 1562, 1794, 2378, 2809, 3067 |
| LmnI | GCTCC | 4 cut(s) | 11, 301, 1750, 2084 |
| Lsp1109I | GCAGC | 5 cut(s) | 697, 1237, 1293, 2686, 3026 |
| LweI | GCATC | 5 cut(s) | 241, 448, 1908, 1973, 2064 |
| MaeI | CTAG | 2 cut(s) | 1176, 1913 |
| MaeII | ACGT | 4 cut(s) | 730, 1219, 1950, 2668 |
| MaeIII | GTNAC | 9 cut(s) | 689, 925, 958, 1229, 1465, 1840, 1936, 2407, 2914 |
| MalI | GATC | 6 cut(s) | 1031, 1564, 1796, 2380, 2811, 3069 |
| MboI | GATC | 6 cut(s) | 1029, 1562, 1794, 2378, 2809, 3067 |
| MflI | RGATCY | 3 cut(s) | 1562, 2378, 3067 |
| MhlI | GDGCHC | 3 cut(s) | 8, 1864, 2877 |
| MlsI | TGGCCA | 2 cut(s) | 1278, 3029 |
| MluNI | TGGCCA | 2 cut(s) | 1278, 3029 |
| MlyI | GAGTC | 2 cut(s) | 644, 2684 |
| MmeI | TCCRAC | 5 cut(s) | 240, 1249, 1540, 2287, 2931 |
| Mox20I | TGGCCA | 2 cut(s) | 1278, 3029 |
| Mph1103I | ATGCAT | 1 cut(s) | 2071 |
| MroI | TCCGGA | 1 cut(s) | 235 |
| MroXI | GAANNNNTTC | 3 cut(s) | 538, 2022, 2445 |
| MscI | TGGCCA | 2 cut(s) | 1278, 3029 |
| MslI | CAYNNNNRTG | 5 cut(s) | 719, 1197, 1554, 3003, 3047 |
| Msp20I | TGGCCA | 2 cut(s) | 1278, 3029 |
| MspA1I | CMGCKG | 1 cut(s) | 2677 |
| MspI | CCGG | 3 cut(s) | 236, 1717, 2461 |
| MspR9I | CCNGG | 1 cut(s) | 3031 |
| Mva1269I | GAATGC | 1 cut(s) | 2728 |
| MvaI | CCWGG | 1 cut(s) | 3031 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 177, 197, 785, 1604, 2234 |
| NcoI | CCATGG | 1 cut(s) | 2142 |
| NdeI | CATATG | 3 cut(s) | 876, 955, 1429 |
| NdeII | GATC | 6 cut(s) | 1029, 1562, 1794, 2378, 2809, 3067 |
| NlaIV | GGNNCC | 4 cut(s) | 297, 393, 1746, 2354 |
| NmuCI | GTSAC | 5 cut(s) | 689, 925, 1229, 1936, 2407 |
| NsiI | ATGCAT | 1 cut(s) | 2071 |
| NspI | RCATGY | 3 cut(s) | 710, 2037, 2069 |
| PaeI | GCATGC | 2 cut(s) | 710, 2037 |
| PaeR7I | CTCGAG | 1 cut(s) | 1676 |
| PagI | TCATGA | 3 cut(s) | 51, 2323, 2425 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1225 |
| PctI | GAATGC | 1 cut(s) | 2728 |
| PdmI | GAANNNNTTC | 3 cut(s) | 538, 2022, 2445 |
| PfeI | GAWTC | 5 cut(s) | 48, 676, 1014, 1241, 1360 |
| PflFI | GACNNNGTC | 1 cut(s) | 634 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1712 |
| PkrI | GCNGC | 8 cut(s) | 193, 196, 199, 712, 1227, 1308, 2676, 3016 |
| PleI | GAGTC | 2 cut(s) | 643, 2684 |
| PpsI | GAGTC | 2 cut(s) | 643, 2684 |
| Ppu21I | YACGTR | 1 cut(s) | 731 |
| PshBI | ATTAAT | 1 cut(s) | 453 |
| Psp124BI | GAGCTC | 1 cut(s) | 8 |
| Psp6I | CCWGG | 1 cut(s) | 3029 |
| PspEI | GGTNACC | 1 cut(s) | 1840 |
| PspGI | CCWGG | 1 cut(s) | 3029 |
| PspN4I | GGNNCC | 4 cut(s) | 297, 393, 1746, 2354 |
| PspPI | GGNCC | 3 cut(s) | 189, 903, 2352 |
| PspXI | VCTCGAGB | 1 cut(s) | 1676 |
| PstI | CTGCAG | 1 cut(s) | 1474 |
| PstNI | CAGNNNCTG | 2 cut(s) | 638, 1469 |
| PsuI | RGATCY | 3 cut(s) | 1562, 2378, 3067 |
| PsyI | GACNNNGTC | 1 cut(s) | 634 |
| RsaI | GTAC | 8 cut(s) | 14, 518, 733, 1058, 1446, 2140, 2156, 2752 |
| RsaNI | GTAC | 8 cut(s) | 13, 517, 732, 1057, 1445, 2139, 2155, 2751 |
| RseI | CAYNNNNRTG | 5 cut(s) | 719, 1197, 1554, 3003, 3047 |
| SacI | GAGCTC | 1 cut(s) | 8 |
| SatI | GCNGC | 8 cut(s) | 192, 195, 198, 711, 1226, 1307, 2675, 3015 |
| Sau3AI | GATC | 6 cut(s) | 1029, 1562, 1794, 2378, 2809, 3067 |
| Sau96I | GGNCC | 3 cut(s) | 189, 903, 2352 |
| ScaI | AGTACT | 2 cut(s) | 518, 2156 |
| SchI | GAGTC | 2 cut(s) | 644, 2684 |
| ScrFI | CCNGG | 1 cut(s) | 3031 |
| SduI | GDGCHC | 3 cut(s) | 8, 1864, 2877 |
| SfaNI | GCATC | 5 cut(s) | 241, 448, 1908, 1973, 2064 |
| SfcI | CTRYAG | 3 cut(s) | 855, 1294, 1470 |
| Sfr274I | CTCGAG | 1 cut(s) | 1676 |
| SlaI | CTCGAG | 1 cut(s) | 1676 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 719, 1197, 1554, 3003, 3047 |
| SmlI | CTYRAG | 3 cut(s) | 566, 822, 1676 |
| SmoI | CTYRAG | 3 cut(s) | 566, 822, 1676 |
| SphI | GCATGC | 2 cut(s) | 710, 2037 |
| SspI | AATATT | 2 cut(s) | 610, 895 |
| SspMI | CTAG | 2 cut(s) | 1176, 1913 |
| SstI | GAGCTC | 1 cut(s) | 8 |
| StyD4I | CCNGG | 1 cut(s) | 3029 |
| StyI | CCWWGG | 2 cut(s) | 2142, 2919 |
| TaiI | ACGT | 4 cut(s) | 733, 1222, 1953, 2671 |
| TaqI | TCGA | 6 cut(s) | 46, 228, 679, 1032, 1677, 2693 |
| TaqII | GACCGA | 1 cut(s) | 2702 |
| TatI | WGTACW | 4 cut(s) | 12, 516, 2154, 2750 |
| TauI | GCSGC | 3 cut(s) | 194, 197, 200 |
| TfiI | GAWTC | 5 cut(s) | 48, 676, 1014, 1241, 1360 |
| TscAI | CASTG | 2 cut(s) | 2608, 2819 |
| TseFI | GTSAC | 5 cut(s) | 689, 925, 1229, 1936, 2407 |
| TseI | GCWGC | 5 cut(s) | 710, 1225, 1306, 2674, 3014 |
| Tsp45I | GTSAC | 5 cut(s) | 689, 925, 1229, 1936, 2407 |
| TspGWI | ACGGA | 4 cut(s) | 208, 246, 1769, 2202 |
| TspRI | CASTG | 2 cut(s) | 2608, 2819 |
| Tth111I | GACNNNGTC | 1 cut(s) | 634 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1712 |
| VspI | ATTAAT | 1 cut(s) | 453 |
| XapI | RAATTY | 4 cut(s) | 397, 1821, 2018, 3052 |
| XceI | RCATGY | 3 cut(s) | 710, 2037, 2069 |
| XhoI | CTCGAG | 1 cut(s) | 1676 |
| XmiI | GTMKAC | 1 cut(s) | 3039 |
| XmnI | GAANNNNTTC | 3 cut(s) | 538, 2022, 2445 |
| XspI | CTAG | 2 cut(s) | 1176, 1913 |
| ZrmI | AGTACT | 2 cut(s) | 518, 2156 |
| Zsp2I | ATGCAT | 1 cut(s) | 2071 |