Rh5CG328300

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000912
Physical Location & Seq
Forward (+)
36688466 .. 36695510
7045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG328300.1

Sequence Viewer

Length: 1038 bp
ATGTCATACTCCATGGATGATTTGATTCAGGCGTTATGTAACATGGCTAAGGACAATGCTCATATTCCTATGGGGTCTCGTACTCATGGCCAGCCTAATGCACCCACAACTGTGAGGAAAGAAATGGCAACTTTTGCTGTGAGATTAAGGAGAGAAAGGAAAGAGATATCTTCGGTGGCGATAATGGGGAAATTTTTTGGTGTTGTTGGAAATTACAACGCTCATCTTGTTGCATATCCTAATATTAATTGGCCCCAAGTTGCTGAACAGTTTGTAGCATCTCTTGGATTGAATTTTCATCCCTATGTTACTTTAAGTAGACGGCAAAGTGAGGCCACTTATATTCGATGGGTTGGTTTGCCGGACCCTCCTTCTTATGCTGAATACCCTATCGCTGCTGTTGATGAGTCTAGTGGTGGTGGTGGTGTTGATGAGTCTGGTGATGAGGAGGAGGAGTCCGTTGGTTGTGATGACGACGCTTTGAGAAGGCACTGTTACTATTCAAGCTTCTCTTATGCAATTTTAGGTAGTCAACTTTTCGTGTCTAATCCACAGCGTCCTGAATATTCATACAAGTGCAACCTTCCCAGCAAGCGTGAAAACTGGCCAGTTGAGTGGAAAGTTTCAAGTTTCCAATTTCAGGGTGTAACTATAGGGGTTCAAACCGCGGCACAAGGGTTTATAGTTAAACTTGAAGATAATCCCTACTTAAATATCAAAGTGCAACTGTTGATAAATGGCTATGATGAACCGGTGCTTTCTCAATCTCTAATGATGCCACAAGAGCTGGAAGGAGTTCAAGACTCCCAGTTTGTTGACCTAGGAGGTCAAAAAGTCTGCTTTCTCTATTCTAGAATGAGAGATTCCAAGTTGCTTGTGACCGCCATCACATTTGGCTTCCAACTCGAAGAGAGAGATAGCTGCTTCAAACTCAAAGCCAAATTTTATCCTACTCGCAACTGGGAATTCCAAAATAAAGCTGGGTCTAAGGCTAAAGTTTGGGGTACGGAACAGATGGTTGGTGCTGTTGTTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130
Pfam Domains
Protein Families
ASL_C Lyase_1

Protein Analysis

345

Amino Acids

38.75

Weight (kDa)

5.67

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_1 PF00206 4 - 96 2.8e-09 Lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 319
AccII CGCG 1 cut(s) 668
AciI CCGC 3 cut(s) 666, 668, 882
AcoI YGGCCR 2 cut(s) 88, 605
AcsI RAATTY 4 cut(s) 191, 292, 941, 965
AfaI GTAC 2 cut(s) 82, 1006
AfiI CCNNNNNNNGG 1 cut(s) 640
AgeI ACCGGT 1 cut(s) 751
AgsI TTSAA 7 cut(s) 292, 504, 627, 662, 695, 800, 928
AjuI GAANNNNNNNTTGG 2 cut(s) 1002, 1034
AleI CACNNNNGTG 1 cut(s) 110
AluBI AGCT 4 cut(s) 507, 787, 921, 980
AluI AGCT 4 cut(s) 507, 787, 921, 980
Alw26I GTCTC 1 cut(s) 81
AoxI GGCC 4 cut(s) 88, 251, 333, 605
ApeKI GCWGC 2 cut(s) 395, 921
ApoI RAATTY 4 cut(s) 191, 292, 941, 965
ArsI GACNNNNNNTTYG 2 cut(s) 794, 826
AseI ATTAAT 1 cut(s) 246
AsiGI ACCGGT 1 cut(s) 751
Asp700I GAANNNNTTC 1 cut(s) 795
AspA2I CCTAGG 1 cut(s) 820
AspS9I GGNCC 2 cut(s) 252, 364
AsuHPI GGTGA 1 cut(s) 452
AvaII GGWCC 1 cut(s) 364
AvrII CCTAGG 1 cut(s) 820
BalI TGGCCA 2 cut(s) 90, 607
BbvI GCAGC 2 cut(s) 382, 908
BccI CCATC 3 cut(s) 342, 893, 1009
BceAI ACGGC 1 cut(s) 338
BcoDI GTCTC 1 cut(s) 81
BfaI CTAG 3 cut(s) 411, 821, 852
BfmI CTRYAG 1 cut(s) 651
BisI GCNGC 3 cut(s) 396, 669, 922
BlnI CCTAGG 1 cut(s) 820
BlsI GCNGC 3 cut(s) 397, 670, 923
Bme18I GGWCC 1 cut(s) 364
BmgT120I GGNCC 2 cut(s) 252, 364
BmiI GGNNCC 2 cut(s) 254, 366
BmrI ACTGGG 2 cut(s) 802, 970
BmsI GCATC 2 cut(s) 287, 765
BmuI ACTGGG 2 cut(s) 802, 970
Bpu10I CCTNAGC 1 cut(s) 48
BsaI GGTCTC 1 cut(s) 81
BsaJI CCNNGG 3 cut(s) 12, 666, 820
BsaWI WCCGGW 1 cut(s) 751
BsaXI ACNNNNNCTCC 2 cut(s) 440, 470
Bsc4I CCNNNNNNNGG 1 cut(s) 640
Bse118I RCCGGY 1 cut(s) 751
Bse1I ACTGG 4 cut(s) 608, 608, 808, 965
BseDI CCNNGG 3 cut(s) 12, 666, 820
BseGI GGATG 2 cut(s) 22, 298
BseLI CCNNNNNNNGG 1 cut(s) 640
BseNI ACTGG 4 cut(s) 608, 608, 808, 965
BseRI GAGGAG 3 cut(s) 461, 464, 467
BseXI GCAGC 2 cut(s) 382, 908
BseYI CCCAGC 2 cut(s) 587, 980
Bsh1236I CGCG 1 cut(s) 668
BshFI GGCC 4 cut(s) 90, 253, 335, 607
BshTI ACCGGT 1 cut(s) 751
BsiSI CCGG 2 cut(s) 362, 752
BslI CCNNNNNNNGG 1 cut(s) 640
BsmAI GTCTC 1 cut(s) 81
BsnI GGCC 4 cut(s) 90, 253, 335, 607
Bso31I GGTCTC 1 cut(s) 81
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 3 cut(s) 666, 668, 882
BspANI GGCC 4 cut(s) 90, 253, 335, 607
BspFNI CGCG 1 cut(s) 668
BspLI GGNNCC 2 cut(s) 254, 366
BspTNI GGTCTC 1 cut(s) 81
BsrFI RCCGGY 1 cut(s) 751
BsrI ACTGG 4 cut(s) 608, 608, 808, 965
BssAI RCCGGY 1 cut(s) 751
BssECI CCNNGG 3 cut(s) 12, 666, 820
BssT1I CCWWGG 2 cut(s) 12, 820
Bst4CI ACNGT 4 cut(s) 112, 270, 494, 729
Bst6I CTCTTC 1 cut(s) 903
BstAPI GCANNNNNTGC 1 cut(s) 134
BstC8I GCNNGC 2 cut(s) 92, 593
BstDEI CTNAG 2 cut(s) 48, 987
BstDSI CCRYGG 2 cut(s) 12, 666
BstF5I GGATG 2 cut(s) 22, 298
BstFNI CGCG 1 cut(s) 668
BstMAI GTCTC 1 cut(s) 81
BstMWI GCNNNNNNNGC 2 cut(s) 134, 784
BstSFI CTRYAG 1 cut(s) 651
BstUI CGCG 1 cut(s) 668
BstV1I GCAGC 2 cut(s) 382, 908
BstXI CCANNNNNNTGG 1 cut(s) 615
BsuRI GGCC 4 cut(s) 90, 253, 335, 607
BtgI CCRYGG 2 cut(s) 12, 666
BtsCI GGATG 2 cut(s) 22, 298
BtsIMutI CAGTG 1 cut(s) 490
Cac8I GCNNGC 2 cut(s) 92, 593
Cfr10I RCCGGY 1 cut(s) 751
Cfr13I GGNCC 2 cut(s) 252, 364
Cfr42I CCGCGG 1 cut(s) 669
CseI GACGC 2 cut(s) 485, 545
Csp6I GTAC 2 cut(s) 81, 1005
CspAI ACCGGT 1 cut(s) 751
CviAII CATG 3 cut(s) 13, 43, 86
CviQI GTAC 2 cut(s) 81, 1005
DdeI CTNAG 2 cut(s) 48, 987
EaeI YGGCCR 2 cut(s) 88, 605
Eam1104I CTCTTC 1 cut(s) 903
EarI CTCTTC 1 cut(s) 903
Eco130I CCWWGG 2 cut(s) 12, 820
Eco31I GGTCTC 1 cut(s) 81
Eco32I GATATC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 364
EcoRI GAATTC 1 cut(s) 965
EcoRV GATATC 1 cut(s) 168
EcoT14I CCWWGG 2 cut(s) 12, 820
ErhI CCWWGG 2 cut(s) 12, 820
FaeI CATG 3 cut(s) 16, 46, 89
FalI AAGNNNNNCTT 2 cut(s) 496, 528
FatI CATG 3 cut(s) 12, 42, 85
FblI GTMKAC 1 cut(s) 319
Fnu4HI GCNGC 3 cut(s) 396, 669, 922
FokI GGATG 2 cut(s) 29, 285
Fsp4HI GCNGC 3 cut(s) 396, 669, 922
FspBI CTAG 3 cut(s) 411, 821, 852
GluI GCNGC 3 cut(s) 396, 669, 922
GsaI CCCAGC 2 cut(s) 591, 984
HaeIII GGCC 4 cut(s) 90, 253, 335, 607
HapII CCGG 2 cut(s) 362, 752
HgaI GACGC 2 cut(s) 485, 545
Hin1II CATG 3 cut(s) 16, 46, 89
HincII GTYRAC 2 cut(s) 533, 817
HindII GTYRAC 2 cut(s) 533, 817
HindIII AAGCTT 1 cut(s) 505
HinfI GANTC 6 cut(s) 25, 407, 434, 455, 803, 863
HpaII CCGG 2 cut(s) 362, 752
HphI GGTGA 1 cut(s) 452
Hpy166II GTNNAC 3 cut(s) 320, 533, 817
Hpy188III TCNNGA 3 cut(s) 560, 800, 852
Hpy8I GTNNAC 3 cut(s) 320, 533, 817
Hpy99I CGWCG 1 cut(s) 479
HpyAV CCTTC 4 cut(s) 381, 480, 593, 785
HpyCH4III ACNGT 4 cut(s) 112, 270, 494, 729
HpyCH4V TGCA 5 cut(s) 101, 233, 518, 579, 724
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 784
HpyF3I CTNAG 2 cut(s) 48, 987
Hsp92II CATG 3 cut(s) 16, 46, 89
KspI CCGCGG 1 cut(s) 669
Lsp1109I GCAGC 2 cut(s) 382, 908
LweI GCATC 2 cut(s) 287, 765
MaeI CTAG 3 cut(s) 411, 821, 852
MaeIII GTNAC 5 cut(s) 38, 307, 494, 646, 877
MboII GAAGA 3 cut(s) 162, 707, 920
MlsI TGGCCA 2 cut(s) 90, 607
MluCI AATT 8 cut(s) 191, 211, 247, 292, 519, 635, 941, 965
MluNI TGGCCA 2 cut(s) 90, 607
MlyI GAGTC 4 cut(s) 416, 443, 464, 797
MmeI TCCRAC 2 cut(s) 187, 925
MnlI CCTC 7 cut(s) 108, 325, 378, 439, 442, 445, 818
Mox20I TGGCCA 2 cut(s) 90, 607
MroXI GAANNNNTTC 1 cut(s) 795
MscI TGGCCA 2 cut(s) 90, 607
MseI TTAA 5 cut(s) 146, 246, 314, 687, 710
MslI CAYNNNNRTG 3 cut(s) 110, 303, 574
Msp20I TGGCCA 2 cut(s) 90, 607
MspA1I CMGCKG 1 cut(s) 668
MspI CCGG 2 cut(s) 362, 752
MvnI CGCG 1 cut(s) 668
MwoI GCNNNNNNNGC 2 cut(s) 134, 784
NcoI CCATGG 1 cut(s) 12
NlaIII CATG 3 cut(s) 16, 46, 89
NlaIV GGNNCC 2 cut(s) 254, 366
NmuCI GTSAC 1 cut(s) 877
OliI CACNNNNGTG 1 cut(s) 110
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 2 cut(s) 25, 863
PinAI ACCGGT 1 cut(s) 751
PkrI GCNGC 3 cut(s) 397, 670, 923
PleI GAGTC 4 cut(s) 415, 442, 463, 797
PpsI GAGTC 4 cut(s) 415, 442, 463, 797
PshBI ATTAAT 1 cut(s) 246
PspFI CCCAGC 2 cut(s) 587, 980
PspN4I GGNNCC 2 cut(s) 254, 366
PspPI GGNCC 2 cut(s) 252, 364
RsaI GTAC 2 cut(s) 82, 1006
RsaNI GTAC 2 cut(s) 81, 1005
RseI CAYNNNNRTG 3 cut(s) 110, 303, 574
SacII CCGCGG 1 cut(s) 669
SaqAI TTAA 5 cut(s) 146, 246, 314, 687, 710
SatI GCNGC 3 cut(s) 396, 669, 922
Sau96I GGNCC 2 cut(s) 252, 364
SchI GAGTC 4 cut(s) 416, 443, 464, 797
SetI ASST 8 cut(s) 509, 529, 585, 789, 822, 829, 923, 982
SfaNI GCATC 2 cut(s) 287, 765
SfcI CTRYAG 1 cut(s) 651
Sfr303I CCGCGG 1 cut(s) 669
SgrBI CCGCGG 1 cut(s) 669
SinI GGWCC 1 cut(s) 364
SmiMI CAYNNNNRTG 3 cut(s) 110, 303, 574
Sse9I AATT 8 cut(s) 191, 211, 247, 292, 519, 635, 941, 965
SsiI CCGC 3 cut(s) 666, 668, 882
SspI AATATT 2 cut(s) 244, 566
SspMI CTAG 3 cut(s) 411, 821, 852
StyI CCWWGG 2 cut(s) 12, 820
TaaI ACNGT 4 cut(s) 112, 270, 494, 729
TaqI TCGA 2 cut(s) 346, 906
TasI AATT 8 cut(s) 191, 211, 247, 292, 519, 635, 941, 965
TauI GCSGC 1 cut(s) 671
TfiI GAWTC 2 cut(s) 25, 863
Tru1I TTAA 5 cut(s) 146, 246, 314, 687, 710
Tru9I TTAA 5 cut(s) 146, 246, 314, 687, 710
TscAI CASTG 1 cut(s) 497
TseFI GTSAC 1 cut(s) 877
TseI GCWGC 2 cut(s) 395, 921
Tsp45I GTSAC 1 cut(s) 877
TspDTI ATGAA 3 cut(s) 287, 558, 762
TspGWI ACGGA 2 cut(s) 448, 1022
TspRI CASTG 1 cut(s) 497
VpaK11BI GGWCC 1 cut(s) 364
VspI ATTAAT 1 cut(s) 246
XapI RAATTY 4 cut(s) 191, 292, 941, 965
XbaI TCTAGA 1 cut(s) 851
XcmI CCANNNNNNNNNTGG 1 cut(s) 977
XmaJI CCTAGG 1 cut(s) 820
XmiI GTMKAC 1 cut(s) 319
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 3 cut(s) 411, 821, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.